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Artículos de revistas sobre el tema "Culture hampers"

Autor: Grafiati
Publicado: 4 de junio de 2021
Última modificación: 1 de febrero de 2022

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1

Gu, Xiaoming. "Advances in Epidemiological Studies of Herpes Zoster." Infection International 4, no. 4 (2015): 112–15. http://dx.doi.org/10.1515/ii-2017-0118.

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Abstract Mycoplasma genitalium (Mg) commonly causes nongonococcal urethritis and cervicitis. Mg is a fastidious bacterium that poses difficulty in time-consuming isolation and culture. Lack of specificity for serological tests also hampers clinical research of Mg. With development of molecular biology, polymerase chain reaction tests, which exhibit high sensitivities and specificities, became primary tools for foundational and clinical studies of Mg.
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2

Mullenders, Jasper, Evelien de Jongh, Anneta Brousali, et al. "Mouse and human urothelial cancer organoids: A tool for bladder cancer research." Proceedings of the National Academy of Sciences 116, no. 10 (2019): 4567–74. http://dx.doi.org/10.1073/pnas.1803595116.

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Bladder cancer is a common malignancy that has a relatively poor outcome. Lack of culture models for the bladder epithelium (urothelium) hampers the development of new therapeutics. Here we present a long-term culture system of the normal mouse urothelium and an efficient culture system of human bladder cancer cells. These so-called bladder (cancer) organoids consist of 3D structures of epithelial cells that recapitulate many aspects of the urothelium. Mouse bladder organoids can be cultured efficiently and genetically manipulated with ease, which was exemplified by creating genetic knockouts
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3

Dodig, Dejan, Miroslav Zorić, Nevena Mitić, et al. "Morphogenetic responses of embryo culture of wheat related to environment culture conditions of the explant donor plant." Scientia Agricola 67, no. 3 (2010): 295–300. http://dx.doi.org/10.1590/s0103-90162010000300007.

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Availability of immature embryos as explants to establish wheat (Triticum aestivum L.) by tissue culture can be limited by climatic factors and the lack of high quality embryos frequently hampers experimentation. This study evaluates the effects of rainfall, various temperature-based variables and sunshine duration on tissue culture response (TCR) traits including callus formation (CF), regenerating calli (RC), and number of plants per embryo (PPE) for 96 wheat genotypes of worldwide origin. The objectives of this study were to evaluate the significance of a particular climatic factor on TCR t
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4

Nakamura, Ren, Daisuke Nakajima, Hironori Sato, Yusuke Endo, Osamu Ohara, and Yusuke Kawashima. "A Simple Method for In-Depth Proteome Analysis of Mammalian Cell Culture Conditioned Media Containing Fetal Bovine Serum." International Journal of Molecular Sciences 22, no. 5 (2021): 2565. http://dx.doi.org/10.3390/ijms22052565.

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A conditioned medium of a cell culture is widely used for various biological applications and frequently analyzed to characterize the functional proteins responsible for observed biological functions. However, a large number of abundant proteins in fetal bovine serum (FBS), usually included in the conditioned medium of a mammalian cell culture medium, hampers in-depth proteomic analysis by liquid chromatography–tandem mass spectrometry (LC-MS/MS). For a deep proteomic analysis of a conditioned medium by LC-MS/MS, we developed a simple albumin depletion approach coupled with data-independent ac
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5

Roobol, Kouwenberg, Denkova, Kanaar, and Essers. "Large Field Alpha Irradiation Setup for Radiobiological Experiments." Methods and Protocols 2, no. 3 (2019): 75. http://dx.doi.org/10.3390/mps2030075.

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The use of alpha particles irradiation in clinical practice has gained interest in the past years, for example with the advance of radionuclide therapy. The lack of affordable and easily accessible irradiation systems to study the cell biological impact of alpha particles hampers broad investigation. Here we present a novel alpha particle irradiation set-up for uniform irradiation of cell cultures. By combining a small alpha emitting source and a computer-directed movement stage, we established a new alpha particle irradiation method allowing more advanced biological assays, including large-fi
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6

Feng, Jing Betty, Leigh Anne Liu, and Chunyan Jiang. "Parochialism and Implications for Chinese Firms’ Globalization." Management and Organization Review 15, no. 4 (2019): 705–36. http://dx.doi.org/10.1017/mor.2019.12.

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ABSTRACTWe explore the meaning of parochialism (xiao nong yi shi, 小农意识) to explain certain paradoxical Chinese managerial behaviors. We discuss how cultural, political, and economic traditions in China formed a salient context to cultivate parochialism. Qualitative data from Chinese and American managers reveal that the conceptual framework of parochialism includes a cognitive dimension of closed-mindedness, a behavioral dimension of self-protection, and a relational dimension of in-group focused social relationship. Parochialism hampers effective globalization of Chinese firms because it nega
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7

Gorpenchenko, Tatiana, Valeria Grigorchuk, Dmitry Bulgakov, Galina Tchernoded, and Victor Bulgakov. "Tempo-Spatial Pattern of Stepharine Accumulation in Stephania Glabra Morphogenic Tissues." International Journal of Molecular Sciences 20, no. 4 (2019): 808. http://dx.doi.org/10.3390/ijms20040808.

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Alkaloids attract great attention due to their valuable therapeutic properties. Stepharine, an aporphine alkaloid of Stephania glabra plants, exhibits anti-aging, anti-hypertensive, and anti-viral effects. The distribution of aporphine alkaloids in cell cultures, as well as whole plants is unknown, which hampers the development of bioengineering strategies toward enhancing their production. The spatial distribution of stepharine in cell culture models, plantlets, and mature micropropagated plants was investigated at the cellular and organ levels. Stepharine biosynthesis was found to be highly
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8

Kirshner, Julia, Kyle J. Thulien, Lorri D. Martin, et al. "A Unique Pre-Clinical Model Providing Access to a Drug Resistant Population within the Multiple Myeloma Stem Cell Niche in a Novel 3-D Culture System for Bone Marrow." Blood 110, no. 11 (2007): 547. http://dx.doi.org/10.1182/blood.v110.11.547.547.

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Abstract Bone marrow (BM), a site of hematopoiesis, is a multicellular tissue with a complex architecture. Multiple myeloma (MM) is an incurable plasma cell malignancy where even patients in remission succumb to an inevitable relapse. While considerable progress has been made towards understanding and treating MM, to date, there is no culture system which can recapitulate the complex interactions within the BM microenvironment. Current failure to grow the MM clone within the context of human microenvironment hampers progress into the understanding of the biology of MM and design of biologicall
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9

Alevra Sarika, Niki, Valéry L. Payen, Maximilien Fléron, et al. "Human Liver-Derived Extracellular Matrix for the Culture of Distinct Human Primary Liver Cells." Cells 9, no. 6 (2020): 1357. http://dx.doi.org/10.3390/cells9061357.

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The lack of robust methods to preserve, purify and in vitro maintain the phenotype of the human liver’s highly specialized parenchymal and non-parenchymal cell types importantly hampers their exploitation for the development of research and clinical applications. There is in this regard a growing interest in the use of tissue-specific extracellular matrix (ECM) to provide cells with an in vitro environment that more closely resembles that of the native tissue. In the present study, we have developed a method that allows for the isolation and downstream application of the human liver’s main cel
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10

Kowalczyk, Maciej Wojciech. "Architectural design contest with social participation as a part of building culture in Europe." Journal of Education Culture and Society 9, no. 2 (2018): 195–200. http://dx.doi.org/10.15503/jecs20182.195.200.

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Thesis. The culture of organising architectural competitions has a long tradition in Europe. The architectural desgin contest is used as a democratic tool for selecting the best design solution. In today’s European building culture there is a constantly growing need for the social input into the design process. In effect, architectural competitions are facing changes. On the one hand, an architectural design contest needs to fulfill the EU’s legal frames. On the other hand, the traditional forms of competions are questioned by society, that is searching for a more transprent selection process.
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11

Alanio, Alexandre, and Stéphane Bretagne. "Challenges in microbiological diagnosis of invasive Aspergillus infections." F1000Research 6 (February 17, 2017): 157. http://dx.doi.org/10.12688/f1000research.10216.1.

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Invasive aspergillosis (IA) has been increasingly reported in populations other than the historical hematology patients and there are new questions about the performance of microbiological tools. Microscopy and culture have been completed by biomarkers, either antigens or DNA, and in blood or respiratory specimens or both. First studied in hematology, the antigen galactomannan performance in serum is low in other patient populations where the pathophysiology of the infection can be different and the prevalence of IA is much lower. DNA detection with polymerase chain reaction (PCR) in blood or
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12

Saraswat, R., M. Kouthanker, S. Kurtarkar, R. Nigam, and V. N. Linshy. "Effect of salinity induced pH changes on benthic foraminifera: a laboratory culture experiment." Biogeosciences Discussions 8, no. 4 (2011): 8423–50. http://dx.doi.org/10.5194/bgd-8-8423-2011.

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Abstract. The coastal water pH varies with salinity. Therefore, to study the effect of salinity induced pH variations on benthic foraminifera, live specimens of Rosalina globularis were subjected to different salinities (10, 15, 20, 25, 30, 35 and 40 ‰) with pH varying from 7.2 to 8.2. A total of 210 specimens were used and the experiment was conducted in replicates. It was observed that the salinity induced pH changes affect the calcification of foraminifera. However the response is not linear. The maximum growth is reported in the specimens kept at 35 ‰ salinity (pH 8.0) while the rest of th
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13

Melzer, Catharina, Roland Jacobs, Thomas Dittmar, et al. "Reversible Growth-Arrest of a Spontaneously-Derived Human MSC-Like Cell Line." International Journal of Molecular Sciences 21, no. 13 (2020): 4752. http://dx.doi.org/10.3390/ijms21134752.

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Life cycle limitation hampers the production of high amounts of primary human mesenchymal stroma-/stem-like cells (MSC) and limits cell source reproducibility for clinical applications. The characterization of permanently growing MSC544 revealed some differentiation capacity and the simultaneous presence of known MSC markers CD73, CD90, and CD105 even after continuous long-term culture for more than one year and 32 passages. The expression of CD13, CD29, CD44, and CD166 were identified as further surface proteins, all of which were also simultaneously detectable in various other types of prima
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14

Kong, David S., Todd A. Thorsen, Jonathan Babb, et al. "Open-source, community-driven microfluidics with Metafluidics." Nature Biotechnology 35, no. 6 (2017): 523–29. http://dx.doi.org/10.1038/nbt.3873.

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Abstract Microfluidic devices have the potential to automate and miniaturize biological experiments, but open-source sharing of device designs has lagged behind sharing of other resources such as software. Synthetic biologists have used microfluidics for DNA assembly, cell-free expression, and cell culture, but a combination of expense, device complexity, and reliance on custom set-ups hampers their widespread adoption. We present Metafluidics, an open-source, community-driven repository that hosts digital design files, assembly specifications, and open-source software to enable users to build
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15

Zappasodi, Roberta, Alessandra Cavanè, Monica Tortoreto, et al. "HSP105 Inhibition Counteracts Key Oncogenic Pathways and Hampers the Growth of Human Aggressive B-Cell Non-Hodgkin Lymphoma." Blood 120, no. 21 (2012): 1562. http://dx.doi.org/10.1182/blood.v120.21.1562.1562.

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Abstract Abstract 1562 Our previous findings have made it clear that the significant clinical efficacy attained by dendritic cell-based vaccination in relapsed B-cell non-Hodgkin lymphoma (B-NHL) patients is firmly associated with multifaceted immunologic responses, including the development of anti-heat shock protein (HSP)105 humoral immunity (Di Nicola et al., Blood 2009 113:18–27; Zappasodi et al., Cancer Res. 2010 70:9062–9072; Zappasodi et al., Blood 2011 118:4421–4430). Human HSP105 is a high-molecular-weight chaperone constitutively expressed at low levels within the cytoplasm, and can
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16

Vaughan, Beverley R., Mike A. Scott, and Elizabeth P. Nachva. "The Application of Growth Factor Stimulants to Improve Ctogenetic Analysis of Patients with Myeloproliferative and Myelodysplastic Syndromes." Blood 104, no. 11 (2004): 2003. http://dx.doi.org/10.1182/blood.v104.11.2003.2003.

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Abstract Conventional cytogenetic methods rely on culturing bone marrow aspirates to obtain suitable and sufficient mitotic figures for G banded analysis. Samples from Myeloproliferative (MPD) and Myelodysplastic (MDS) patients often have increased failure rates due to reduced growth and poor morphology all of which hampers the conventional karyotyping investigation . The aim of this study was to investigate whether; The quality and quantity of metaphase cells from MPD and MDS patients could be improved. To assess whether the applied method(s) led to clonal selection. Three culture systems usi
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17

Paul, Nibedita, Nadia Nusrat, Md Rafiqul Islam, Farhana Rahman, and Neshwa Rahman. "Clinico-Laboratory Profile and Drug Sensitivity Pattern in Urinary Tract Infection of Children in a Tertiary Care Hospital." Bangladesh Journal of Child Health 43, no. 2 (2019): 74–79. http://dx.doi.org/10.3329/bjch.v43i2.42549.

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Background: Urinary tract infection (UTI) is common in children. About 7% girls and 2% of boys suffer from symptomatic, culture positive UTI by 6 years of age. These children present with poor feeding, irritability, vomiting, fever, abdominal pain or failure to thrive. Now a days antibiotic resistance is a global problem which hampers appropriate treatment of urinary tract infection in children. This study was done to see clinical profile, common pathogens and their drug sensitivity pattern in UTI.
 Methodology: It is a prospective study conducted in Delta Medical College & Hospital,
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18

Stahr, Pascal-L., Rekha Grewal, Gunter P. Eckert, and Cornelia M. Keck. "Investigating hesperetin nanocrystals with tailor-made sizes for the prevention and treatment of Alzheimer’s disease." Drug Delivery and Translational Research 11, no. 2 (2021): 659–74. http://dx.doi.org/10.1007/s13346-020-00888-0.

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Abstract Poor aqueous solubility of drug substances is associated with poor bioavailability and thus hampers the effective use of many potent active pharmaceutical ingredients. Various strategies to overcome poor solubility are available, whereby drug nanocrystals represent one of the most powerful formulation strategies to enhance the kinetic solubility and dissolution rate of poorly soluble drugs. Nanocrystals are simply obtained by milling large-sized drug powders to sizes < 1 µm. The so obtained nanocrystals possess an increased dissolution rate and kinetic solubility when compared with
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19

Dao, Mo A., Ami J. Shah, Gay M. Crooks, and Jan A. Nolta. "Engraftment and Retroviral Marking of CD34+ and CD34+CD38− Human Hematopoietic Progenitors Assessed in Immune-Deficient Mice." Blood 91, no. 4 (1998): 1243–55. http://dx.doi.org/10.1182/blood.v91.4.1243.

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Abstract Retroviral-mediated transduction of human hematopoietic stem cells to provide a lifelong supply of corrected progeny remains the most daunting challenge to the success of human gene therapy. The paucity of assays to examine transduction of pluripotent human stem cells hampers progress toward this goal. By using the beige/nude/xid (bnx)/hu immune-deficient mouse xenograft system, we compared the transduction and engraftment of human CD34+progenitors with that of a more primitive and quiescent subpopulation, the CD34+CD38− cells. Comparable extents of human engraftment and lineage devel
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20

Leroy, Frédéric, and Luc De Vuyst. "A Combined Model To Predict the Functionality of the Bacteriocin-Producing Lactobacillus sakei Strain CTC 494." Applied and Environmental Microbiology 69, no. 2 (2003): 1093–99. http://dx.doi.org/10.1128/aem.69.2.1093-1099.2003.

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ABSTRACT The use of bacteriocin-producing lactic acid bacteria for improved food fermentation processes seems promising. However, lack of fundamental knowledge about the functionality of bacteriocin-producing strains under food fermentation conditions hampers their industrial use. Predictive microbiology or a mathematical estimation of microbial behavior in food ecosystems may help to overcome this problem. In this study, a combined model was developed that was able to estimate, from a given initial situation of temperature, pH, and nutrient availability, the growth and self-inhibition dynamic
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21

Tay, A. K., A. Riley, R. Islam, et al. "The culture, mental health and psychosocial wellbeing of Rohingya refugees: a systematic review." Epidemiology and Psychiatric Sciences 28, no. 5 (2019): 489–94. http://dx.doi.org/10.1017/s2045796019000192.

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AbstractAimsDespite the magnitude and protracted nature of the Rohingya refugee situation, there is limited information on the culture, mental health and psychosocial wellbeing of this group. This paper, drawing on a report commissioned by the United Nations High Commissioner for Refugees (UNHCR), aims to provide a comprehensive synthesis of the literature on mental health and psychosocial wellbeing of Rohingya refugees, including an examination of associated cultural factors. The ultimate objective is to assist humanitarian actors and agencies in providing culturally relevant Mental Health an
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Foti, Chrysanthi, and Ourania Pavli. "High-Efficiency Agrobacterium rhizogenes-Mediated Transgenic Hairy Root Induction of Lens culinaris." Agronomy 10, no. 8 (2020): 1170. http://dx.doi.org/10.3390/agronomy10081170.

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Previous efforts to transform lentil have been considerably hampered by the crop’s recalcitrant nature, giving rise to particularly low transformation and regeneration frequencies. This study aimed at optimizing an Agrobacterium rhizogenes-mediated transformation protocol for the generation of composite lentil plantlets, comprised of transgenic hairy roots and wild-type shoots. Transformation was performed by inoculating the cut hypocotyl of young lentil seedlings, while optimization involved the use of different bacterial strains, namely R1000, K599 and Arqua, and protocols differing in media
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23

Verhoeyen, Els, Maciej Wiznerowicz, Delphine Olivier, et al. "Novel lentiviral vectors displaying “early-acting cytokines” selectively promote survival and transduction of NOD/SCID repopulating human hematopoietic stem cells." Blood 106, no. 10 (2005): 3386–95. http://dx.doi.org/10.1182/blood-2004-12-4736.

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AbstractA major limitation of current lentiviral vectors (LVs) is their inability to govern efficient gene transfer into quiescent cells, such as human CD34+ cells, that reside in the G0 phase of the cell cycle and that are highly enriched in hematopoietic stem cells. This hampers their application for gene therapy of hematopoietic cells. Here, we designed novel LVs that overcome this restriction by displaying “early-acting cytokines” on their surface. Display of thrombopoietin, stem cell factor, or both cytokines on the LV surface allowed efficient gene delivery into quiescent cord blood CD34
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24

Spiegelberg, T., and J. O. Bishop. "Tissue-specific gene expression in mouse hepatocytes cultured in growth-restricting medium." Molecular and Cellular Biology 8, no. 8 (1988): 3338–44. http://dx.doi.org/10.1128/mcb.8.8.3338.

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Culture conditions which maintain hepatocytes in their in vivo state are not known. This hampers the study of liver gene expression and of direct responses of liver genes to hormonal stimulation. We argued that hepatocytes that were unable to divide might retain in vivo characteristics. We therefore plated mouse (BALB/c) hepatocytes on plastic dishes in medium lacking arginine and measured the levels and transcription rates of six tissue-specific mRNAs over a period of days. Alpha-fetoprotein mRNA began to accumulate at about 48 h of culture, and transcription could sometimes be detected after
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25

Spiegelberg, T., and J. O. Bishop. "Tissue-specific gene expression in mouse hepatocytes cultured in growth-restricting medium." Molecular and Cellular Biology 8, no. 8 (1988): 3338–44. http://dx.doi.org/10.1128/mcb.8.8.3338-3344.1988.

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Culture conditions which maintain hepatocytes in their in vivo state are not known. This hampers the study of liver gene expression and of direct responses of liver genes to hormonal stimulation. We argued that hepatocytes that were unable to divide might retain in vivo characteristics. We therefore plated mouse (BALB/c) hepatocytes on plastic dishes in medium lacking arginine and measured the levels and transcription rates of six tissue-specific mRNAs over a period of days. Alpha-fetoprotein mRNA began to accumulate at about 48 h of culture, and transcription could sometimes be detected after
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26

Hu, Wei, Qiqi Lu, Guohua Zhong, Meiying Hu, and Xin Yi. "Biodegradation of Pyrethroids by a Hydrolyzing Carboxylesterase EstA from Bacillus cereus BCC01." Applied Sciences 9, no. 3 (2019): 477. http://dx.doi.org/10.3390/app9030477.

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Microbial degradation has been considered as a rapid, green, and cost-effective technique to reduce insecticide pollutions in a contaminated environment. However, the instability and low efficacy of non-indigenous microorganisms hampers their further exploitation when being introduced into a real environmental matrix. In order to overcome the restriction that these functional microorganisms are under, we investigated the optimal conditions to improve the pyrethroid-degrading ability of one previously isolated bacterium Bacillus cereus BCC01, where 9.6% of the culture suspension (with cell dens
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Körholz, Katharina, Johannes Ridinger, Damir Krunic, et al. "Broad-Spectrum HDAC Inhibitors Promote Autophagy through FOXO Transcription Factors in Neuroblastoma." Cells 10, no. 5 (2021): 1001. http://dx.doi.org/10.3390/cells10051001.

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Depending on context and tumor stage, deregulation of autophagy can either suppress tumorigenesis or promote chemoresistance and tumor survival. Histone deacetylases (HDACs) can modulate autophagy; however, the exact mechanisms are not fully understood. Here, we analyze the effects of the broad-spectrum HDAC inhibitors (HDACi) panobinostat and vorinostat on the transcriptional regulation of autophagy with respect to autophagy transcription factor activity (Transcription factor EB—TFEB, forkhead boxO—FOXO) and autophagic flux in neuroblastoma cells. In combination with the late-stage autophagic
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28

Singh, Anand Narain, Ram Chand, and Amandeep Kaur. "Little seed canary grass (Phalaris minor Retz.) promoting own growth functional traits but suppressing of wheat crop (Triticum aestivum L.) at vegetative stage: An ecological assessment." Annals of Plant Sciences 6, no. 11 (2017): 1742. http://dx.doi.org/10.21746/aps.2017.6.11.5.

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Weed-crop interaction in the agro-ecosystems is a serious biological concern which hampers production and yield of the crops. Present study conducted to assess growth functional traits of selected weed and crop species under weed-crop interaction experiment that was established in a Latin square matrix model (LSM) in the backside of Department of Botany in a fenced dome. We investigated the ability of little seed canary grass that how it suppresses wheat crop growth at vegetative stage under mono- and mixed culture combinations. Data sampling for selected growth traits (Shoot height, Leaf area
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Wishnia, Jodi, and Jane Goudge. "Impact of financial management centralisation in a health system under austerity: a qualitative study from South Africa." BMJ Global Health 5, no. 10 (2020): e003524. http://dx.doi.org/10.1136/bmjgh-2020-003524.

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IntroductionInternational calls for universal health coverage (UHC) have led many countries to implement health sector reforms, however, since the 2008 global recession, economic growth has slowed in many lower-income and middle-income countries. In a renewed interest in public financial management (PFM), international organisations have emphasised the importance of giving spending control to those responsible for healthcare. However, centralisation is a common response when there is a need to cut expenditure due to a reduced budget; yet failure to decentralise often hampers the achievement of
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Mao, Hanwen, Wenli Liu, Zhe Gen, et al. "Cytotoxic Effects of CML28 Specific T Cells on Acute Leukemia Cells In Vitro." Blood 110, no. 11 (2007): 4886. http://dx.doi.org/10.1182/blood.v110.11.4886.4886.

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Abstract The antigen-specific cytotoxic T lymphocyte activated by antigen presenting cell is widely used in cell immunotherapy recently. CML28, which was screened from chronic myelogenous leukemia (CML) patients, was reported to be a specific tumour antigen and over-expressed on CML cells and acute leukemia cells. Therefore, CML28 could be a potential target for leukemia treatment. Dendritic cells (DC) are the most important antigen present cells, but it is hard to isolate and culture DCs for clinical use, which hampers the specific cell immunotherapy. Our investigation aimed to study the cyto
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31

van de Garde, Martijn D. B., Suzan D. Pas, Guido van der Net, et al. "Hepatitis E Virus (HEV) Genotype 3 Infection of Human Liver Chimeric Mice as a Model for Chronic HEV Infection." Journal of Virology 90, no. 9 (2016): 4394–401. http://dx.doi.org/10.1128/jvi.00114-16.

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ABSTRACTGenotype 3 (gt3) hepatitis E virus (HEV) infections are emerging in Western countries. Immunosuppressed patients are at risk of chronic HEV infection and progressive liver damage, but no adequate model system currently mimics this disease course. Here we explore the possibilities ofin vivoHEV studies in a human liver chimeric mouse model (uPA+/+Nod-SCID-IL2Rγ−/−) next to the A549 cell culture system, using HEV RNA-positive EDTA-plasma, feces, or liver biopsy specimens from 8 immunocompromised patients with chronic gt3 HEV. HEV from feces- or liver-derived inocula showed clear virus pro
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32

Ruan, Jiwei, Guoxian Wang, Gongwei Ning, et al. "Longer Duration of Short-day Treatment Is Required to Advance Flowering and Fruiting of Decaploid Strawberry ‘Tokun’." HortScience 55, no. 1 (2020): 30–34. http://dx.doi.org/10.21273/hortsci14507-19.

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Strawberry ‘Tokun’ (2n = 10x =70) is a unique cultivar with special flavors, but its late maturity hampers its extension. To advance flowering and fruiting of this decaploid strawberry, the effects of short-day combined with extra nitrogen (N) nutrition treatments on strawberry ‘Tokun’ plants were studied. Runner plantlets of strawberry ‘Tokun’ were harvested and rooted in tray plugs in June 2016, 2017, and 2018, and established plants were conditioned with short-day (SD; 10 hours) and extra N nutrition. The conditioned plants were transplanted into a tabletop substrate culture system in a pla
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33

OKURA, Sae. "The Political Underrepresentation of People with Disabilities in the Japanese Diet." Social Science Japan Journal 24, no. 2 (2021): 369–96. http://dx.doi.org/10.1093/ssjj/jyab024.

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Abstract This paper examines the political underrepresentation of people with disabilities in the Japanese Diet and the conditions that allow their participation in the body. The paper addresses the following three research questions: (a) how are people with disabilities represented in contemporary Japan and the EU member states, and what are the differences between them?; (b) why have people with disabilities been underrepresented in Japan? and (c) how do candidates with disabilities but lacking typical political careers become members of the Diet? This paper further discusses the electoral s
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34

Franceschini, Natasja, Bas Verbruggen, Marianna A. Tryfonidou, et al. "Transformed Canine and Murine Mesenchymal Stem Cells as a Model for Sarcoma with Complex Genomics." Cancers 13, no. 5 (2021): 1126. http://dx.doi.org/10.3390/cancers13051126.

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Sarcomas are rare mesenchymal tumors with a broad histological spectrum, but they can be divided into two groups based on molecular pathology: sarcomas with simple or complex genomics. Tumors with complex genomics can have aneuploidy and copy number gains and losses, which hampers the detection of early, initiating events in tumorigenesis. Often, no benign precursors are known, which is why good models are essential. The mesenchymal stem cell (MSC) is the presumed cell of origin of sarcoma. In this study, MSCs of murine and canine origin are used as a model to identify driver events for sarcom
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HØEGH-GULDBERG, Olga, and Sabrina SEELER. "National Identity as Driver of Tourism Development — the Study of Norway." Arctic and North, no. 42 (March 23, 2021): 32–59. http://dx.doi.org/10.37482/issn2221-2698.2021.42.32.

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The urgent global need to decrease the dependence on natural resource extraction and find solutions for a sustainable future is also reflected in policies prioritized by the Norwegian government. Among others, tourism has been defined as a promising alternative for future economic development. Tourism in Norway has not remained unaffected by the global growth in international tourist arrivals. This growth is often neither geographically nor temporally equally apportioned, which hampers tourism’s transformative power of generating year-round and well-distributed income. Further, tourists are no
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36

Yang, Chongguang, Yangyi Zhang, Yuan Jiang, et al. "82125 Multiple epidemics of multidrug-resistant tuberculosis revealed by spatial disease mapping and whole-genome sequencing analysis in urban China." Journal of Clinical and Translational Science 5, s1 (2021): 5–6. http://dx.doi.org/10.1017/cts.2021.416.

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ABSTRACT IMPACT: Our study will integrate state-of-the-art methods in pathogen genomics, epidemiology, and geospatial analysis to identify both host- and pathogen-factors driving the MDR-TB transmission and the study outcome can inform the design of targeted interventions OBJECTIVES/GOALS: The emergence of multidrug-resistant tuberculosis (MDR-TB) poses serious challenges for the global eradication of tuberculosis. Recent research has shown that transmission is now the dominant driver of MDR-TB. However, our limited understanding of where and among whom MDR-TB is transmitted hampers efforts to
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37

Ferner, Robin E., and Sarah E. McDowell. "Criminal proceedings will hamper calls for open culture." BMJ 331, no. 7527 (2005): 1272.3. http://dx.doi.org/10.1136/bmj.331.7527.1272-b.

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38

Brito, B., J. M. Palacios, J. Imperial, and T. Ruiz-Argüeso. "Engineering the Rhizobium leguminosarum bv. viciae Hydrogenase System for Expression in Free-Living Microaerobic Cells and Increased Symbiotic Hydrogenase Activity." Applied and Environmental Microbiology 68, no. 5 (2002): 2461–67. http://dx.doi.org/10.1128/aem.68.5.2461-2467.2002.

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ABSTRACT Rhizobium leguminosarum bv. viciae UPM791 induces hydrogenase activity in pea (Pisum sativum L.) bacteroids but not in free-living cells. The symbiotic induction of hydrogenase structural genes (hupSL) is mediated by NifA, the general regulator of the nitrogen fixation process. So far, no culture conditions have been found to induce NifA-dependent promoters in vegetative cells of this bacterium. This hampers the study of the R. leguminosarum hydrogenase system. We have replaced the native NifA-dependent hupSL promoter with the FnrN-dependent fixN promoter, generating strain SPF25, whi
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39

Murín, R., K. Mészáros, P. Nemeček, R. Kuna, and J. Faragó. "Regeneration of immature and mature embryos from diverse sets of wheat genotypes using media containing different auxins." Acta Agronomica Hungarica 60, no. 2 (2012): 97–108. http://dx.doi.org/10.1556/aagr.60.2012.2.2.

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The effect of explant type (immature vs. mature embryos) and two auxin types (2,4-dichlorophenoxyacetic acid vs. Dicamba) on the callogenesis and plant regeneration ability of 26 wheat cultivars was studied. In general, the callus induction, plant regeneration and shoot formation frequencies were higher in mature embryo-derived cultures as compared to immature ones on media originally developed for mature wheat embryo cultures. In both culture types, the auxin Dicamba was found to be more efficient, especially when mature embryos were cultured. The separation of means using Duncan’s multiple r
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40

Dobbs, L. G., M. S. Pian, M. Maglio, S. Dumars, and L. Allen. "Maintenance of the differentiated type II cell phenotype by culture with an apical air surface." American Journal of Physiology-Lung Cellular and Molecular Physiology 273, no. 2 (1997): L347—L354. http://dx.doi.org/10.1152/ajplung.1997.273.2.l347.

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The study of differentiated functions of alveolar type II cells has been hampered because of the lack of good in vitro systems. We report that culture of type II cells on collagen gels with an apical surface exposed to air promotes expression of differentiated type II cell characteristics. Cells cultured in this manner are cuboidal, contain lamellar bodies, and produce tubular myelin; in addition, they secrete phosphatidylcholine in response to exogenous ATP. Cultures contain mRNA for surfactant proteins A, B, and C and surfactant proteins A, B, and D. In contrast, when type II cells are cultu
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41

Ferré, L., C. Fresno, M. Kjelland, and P. Ross. "50 SURVIVAL OF SEXED IVF-DERIVED BOVINE EMBRYOS FROZEN AT DIFFERENT PREIMPLANTATION STAGES OF DEVELOPMENT." Reproduction, Fertility and Development 29, no. 1 (2017): 132. http://dx.doi.org/10.1071/rdv29n1ab50.

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The ability to freeze in vitro-produced bovine embryos with a high post-thaw viability is still problematic and hampers logistics of on-farm embryo transfer. The objectives of this experiment were to compare different stages of development, freezing methods, and addition of cytoskeletal stabilisers (cytochalasin-B) before freezing. Ovaries were collected from an abattoir and oocytes aspirated from 2- to 6-mm follicles. Cumulus-oocyte complexes containing compact and complete cumulus cell layers were selected and matured in groups of 50 in 400 µL of M199 medium supplemented with ALA-glutamine (
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42

Hopfer, Helmut, Günter Vollmer, Clifford A. Rinehart Jr., and David G. Kaufman. "Basement membrane induced differentiation of HEC-1B(L) endometrial adenocarcinoma cells affects both morphology and gene expression." Biochemistry and Cell Biology 74, no. 2 (1996): 165–77. http://dx.doi.org/10.1139/o96-017.

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In vitro studies of endometrial carcinogenesis have been hampered by dedifferentiation of the cells in culture. Using the endometrial carcinoma cell line HEC-1B(L), we aimed to establish and characterize culture conditions mat preserve a more differentiated state of the tumor cells. HEC-1B(L) cells grown in a serum-free defined medium on plastic (PL/SFDM) on top of a reconstituted basement membrane (Matrigel™, MG/SFDM) or in a thick layer of Matrigel showed pronounced morphological differentiation as compared with HEC-1B(L) cells cultured on plastic in a medium containing serum (PL/10% FCS). F
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43

Medvinsky, Alexander. "Development of Hematopoietic Stem Cells in the Human Embryo." Blood 132, Supplement 1 (2018): SCI—13—SCI—13. http://dx.doi.org/10.1182/blood-2018-99-109423.

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Abstract Haematopoietic stem cells (HSCs) develop intra-embryonically in the aorta-gonad-mesonephros (AGM) region, more specifically in the ventral domain of the embryonic dorsal aorta. This process is highly conserved across vertebrate species. Key transcription factors and signalling pathways involved in HSC development have been identified using various model organisms. As a mammal, the mouse represents an excellent model for understanding HSC development in the human embryo. However, in utero development significantly reduces accessibility of the embryo for experimentation, which hampers a
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44

Gastens, Martin H., Kristin Goltry, Wolfgang Prohaska, et al. "Good Manufacturing Practice-Compliant Expansion of Marrow-Derived Stem and Progenitor Cells for Cell Therapy." Cell Transplantation 16, no. 7 (2007): 685–96. http://dx.doi.org/10.3727/000000007783465172.

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Ex vivo expansion is being used to increase the number of stem and progenitor cells for autologous cell therapy. Initiation of pivotal clinical trials testing the efficacy of these cells for tissue repair has been hampered by the challenge of assuring safe and high-quality cell production. A strategy is described here for clinical-scale expansion of bone marrow (BM)-derived stem cells within a mixed cell population in a completely closed process from cell collection through postculture processing using sterile connectable devices. Human BM mononuclear cells (BMMNC) were isolated, cultured for
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45

Weiss, T., H. Meister, M. Weller, C. Sentman, and P. Roth. "PL2.1 Exploiting the DNAM-1 system for chimeric antigen receptor (CAR) T cell therapy of glioblastoma." Neuro-Oncology 21, Supplement_3 (2019): iii2. http://dx.doi.org/10.1093/neuonc/noz126.002.

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Abstract BACKGROUND Cancer immunotherapy with genetically engineered T cells that express a chimeric antigen receptor (CAR) has led to impressive responses in extracranial malignancies and is also explored against glioblastoma. However, CAR T cell strategies that are currently being explored against glioblastoma target single tumor antigens, which are non-homogeneously expressed and are prone to antigen escape. Furthermore, the immunosuppressive brain tumor microenvironment hampers anti-tumor efficacy. METHODS By immunohistochemistry and flow cytometry, we investigated the expression of CD155
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Kaur, Pavinder, Brenda Ward, Baisakhi Saha, et al. "Human Breast Cancer Histoid." Journal of Histochemistry & Cytochemistry 59, no. 12 (2011): 1087–100. http://dx.doi.org/10.1369/0022155411423680.

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Progress in our understanding of heterotypic cellular interaction in the tumor microenvironment, which is recognized to play major roles in cancer progression, has been hampered due to unavailability of an appropriate in vitro co-culture model. The aim of this study was to generate an in vitro 3-dimensional human breast cancer model, which consists of cancer cells and fibroblasts. Breast cancer cells (UACC-893) and fibroblasts at various densities were co-cultured in a rotating suspension culture system to establish co-culture parameters. Subsequently, UACC-893, BT.20, or MDA.MB.453 were co-cu
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Mateos, Rosa María, Gema Jiménez, Carmen Álvarez-Gil, et al. "Excess Hydrocortisone Hampers Placental Nutrient Uptake Disrupting Cellular Metabolism." BioMed Research International 2018 (October 9, 2018): 1–11. http://dx.doi.org/10.1155/2018/5106174.

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Low birth weight increases neonatal morbidity and mortality, and surviving infants have increased risk of metabolic and cardiovascular disturbances later in life, as well as other neurological, psychiatric, and immune complications. A gestational excess of glucocorticoids (GCs) is a well-known cause for fetal growth retardation, but the biological basis for this association remains elusive. Placental growth is closely related to fetal growth. The placenta is the main regulator of nutrient transport to the fetus, resulting from the difference between placental nutrient uptake and the placenta’s
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48

Brotodjojo, R. R. Rukmowati, Chimayatus Solichah, Anindita Widyaningtyas, and Danar Wicaksono. "Effects of Culture Media on Viability of Beauveria Bassiana and Its Pathogenicity Against Coffee Bean Borer (Hyphotenemus Hampei)." Proceeding International Conference on Science and Engineering 3 (April 30, 2020): 49–53. http://dx.doi.org/10.14421/icse.v3.467.

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Coffee bean borer (Hypothenemus hampei) attacks coffee when it is still in the plantation until it is in the storage that reduce the quality of coffee. To avoid the negative impact of chemical insecticides, biological control is carried out by using entomopathogen Beauveria bassiana. The aims of this research were to study the effects of culture media on viability of B. bassiana and its pathogenicity in controlling H. hampei. Laboratory experiment was arranged in Completely Random Design (CRD). This research used 8 (eight) treatments namely KO: Distilled water, Kk: Lamda Sihalothrin 25 EC (Che
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Borel Rinkes, Inne H. M., Mehmet Toner, Ronald G. Tompkins, and Martin L. Yarmush. "An Extracorporeal Microscopy Perfusion Chamber for On-Line Studies of Environmental Effects on Cultured Hepatocytes." Journal of Biomechanical Engineering 116, no. 2 (1994): 135–39. http://dx.doi.org/10.1115/1.2895711.

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The development of bioartificial-hybrid organ support systems is hampered by the lack of knowledge on the effects of different (in vivo) environments on cells during extracorporeal perfusion. In the present study, a perfusion chamber was designed for continuous monitoring of cultured cells during perfusion with media, as well as during plasma perfusion in an extracorporeal circuit. Chamber characterization showed satisfactory thermal and perfusion profiles and no major pH fluctuations. Further testing was performed with hepatocytes that were cultured in between two collagen layers, a configura
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50

Somfai, T., N. T. Hiep, K. Kikuchi, and Y. Hirao. "29 The effect of vitrification at the immature stage on DNA methylation in porcine oocytes and its relevance to subsequent embryo development." Reproduction, Fertility and Development 33, no. 2 (2021): 122. http://dx.doi.org/10.1071/rdv33n2ab29.

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Oocyte vitrification is an important approach for invitro gene banking of female germplasm; however, in pigs, it hampers embryo development. In cattle, vitrification at the MII stage was reported to alter epigenetic status in oocytes and even in subsequently developing embryos (Chen et al. 2016 Theriogenology 86, 868-878). The present study investigated the effect of vitrification at the immature stage of porcine oocytes on DNA methylation status and its relevance to subsequent embryo development. Immature cumulus–oocyte complexes were vitrified in microdrops and warmed (vitrified group) or tr
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