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Artículos de revistas sobre el tema "Direct bioautography"

Autor: Grafiati
Publicado: 7 de junio de 2025
Última modificación: 2 de agosto de 2025

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1

Anuar, Nadia, Sadia Sultan, and Kamran Ashraf. "An Overview of Antimicrobial and Antioxidant Bioautography Method Analysis: Cosmos caudatus and Orthosiphon stamineus." International Journal of Pharmaceuticals, Nutraceuticals and Cosmetic Science 5, no. 1 (2022): 1–12. http://dx.doi.org/10.24191/ijpnacs.v5i1.01.

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Medicinal herbs such as Cosmos caudatus and Orthosiphon stamineus are sources of a huge number of bioactive compounds with antioxidant and antimicrobial properties. C. caudatus plant has bioactive compounds such as phenolics, flavonoids, and terpenoids. On the other hand, phytochemicals from O. stamineus have been shown to have a wide range of traditional applications due to their antioxidant and antibacterial characteristics. The bioautography method is used to isolate active molecules on chromatogram followed by the chemical and biological detection system. In antimicrobial bioautography, ag
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2

Vishwa, Patel1* Niyati Acharya2. "Application of TLC Bioautography for Natural Bioactive Screening." International Journal of Pharmaceutical Sciences 3, no. 5 (2025): 1376–86. https://doi.org/10.5281/zenodo.15378848.

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Bioautography is a mean of targeted-directed isolation of active molecules on chromatogram. Bioautography is technique to detect substances affecting the growth rates to test organisms in complex mixtures and matrices. The antimicrobial, antioxidant and enzyme inhibitory activities can be performed on TLC bioautography. Bioautography methods are divided into three categories. 1)Agar diffusion or contact bioautography 2) Immersion or agar-overlay bioautography 3) Direct bioautography. High throughput method enabling analyses of many samples in parallel and the comparison of their activity. Both
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3

Móricz, Ágnes M., and Péter G. Ott. "Direct Bioautography as a High-Throughput Screening Method for the Detection of Antibacterial Components from Plant Sources." Journal of AOAC INTERNATIONAL 98, no. 4 (2015): 850–56. http://dx.doi.org/10.5740/jaoacint.sge1-moricz.

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Abstract The applicability of direct bioautography, the combination of planar chromatography with antimicrobial assay, is demonstrated with special emphasis on its recent developments such as BioArena and the use of genetically modified luminescent bacteria. Its methodological advancement is put into a historical perspective. In comparison with other commonly used antimicrobial susceptibility tests, the main advantage of direct bioautography resides in its simplicity, rapidity, and ability to detect separated individual matrix components exhibiting antimicrobial activity in situ. It is confirm
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4

Zang, Yichao, Zhihong Cheng, and Tao Wu. "TLC Bioautography on Screening of Bioactive Natural Products: An Update Review." Current Analytical Chemistry 16, no. 5 (2020): 545–56. http://dx.doi.org/10.2174/1573411015666181224145346.

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Background: TLC bioautography is a hyphenated technique combining planar chromatographic separation and in situ biological activity detection. This coupled method has been receiving much attention in screening bio-active natural products because of its properties of being simple, rapid, inexpensive, and effective. Methods: The recent progress in the development of method of TLC bioautography for detecting antimicrobial and enzyme inhibitory activities dating between 2012 and early 2018 has been reviewed. The applications of this method in biological screening of natural products were also pres
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5

Horváth, Györgyi, Noémi Jámbor, Erika Kocsis, et al. "Role of Direct Bioautographic Method for Detection of Antistaphylococcal Activity of Essential Oils." Natural Product Communications 6, no. 9 (2011): 1934578X1100600. http://dx.doi.org/10.1177/1934578x1100600939.

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The aim of the present study was the chemical characterization of some traditionally used and therapeutically relevant essential oils (thyme, eucalyptus, cinnamon bark, clove, and tea tree) and the optimized microbiological investigation of the effect of these oils on clinical isolates of methicillin-resistant Staphylococcus aureus (MRSA) and methicillin-susceptible S. aureus (MSSA). The chemical composition of the oils was analyzed by TLC, and controlled by gas chromatography (GC) and gas chromatography/mass spectrometry (GC/MS). The antibacterial effect was investigated using a TLC-bioautogr
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6

Grzelak, Edyta M., Wioleta Jesionek, Barbara Majer-Dziedzic, and Irena M. Choma. "Applications of Novel Direct Bioautography Tests for Analysis of Antimicrobials: A Review." Journal of AOAC INTERNATIONAL 96, no. 6 (2013): 1167–74. http://dx.doi.org/10.5740/jaoacint.sgegrzelak.

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Abstract TLC coupled to direct bioautography detection can be applied to the analysis of various antimicrobial agents in complex matrixes. Because of the lack of commercially available microbiological detection methods, two direct bioautography tests were developed in our laboratory to be used after TLC separation. One method was based on Gram-positive bacteria, Bacillus subtilis, and the other on Gram-negative bacteria, Escherichia coli. These tests can be used for detection and determination of wide spectrum of antimicrobials as well as for other, nontypical purposes, such as choosing the be
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7

Horváth, Györgyi, Kamilla Ács, and Béla Kocsis. "TLC-Direct Bioautography for Determination of Antibacterial Activity of Artemisia adamsii Essential Oil." Journal of AOAC INTERNATIONAL 96, no. 6 (2013): 1209–13. http://dx.doi.org/10.5740/jaoacint.sgehorvath.

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Abstract The aim of the present study was the chemical characterization of the essential oil of a Mongolian medicinal plant, Artemisia adamsii Besser, and the investigation of the antibacterial effect of its oil on different human pathogenic bacteria (Staphylococcus aureus, methicillin-resistant S. aureus, and S. epidermidis). The chemical composition of the oil was established by GC and GC/MS. Direct bioautography was used for detecting the antibacterial activity of the essential oil. The result of GC experiments showed that α-thujone was the main component (64.4%) of the oil, while the amoun
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8

Patil, Anita Surendra, Hariprasad Madhukarrao Paikrao, Ankit Subhash Kale, and Surendra Raghoba Manik. "A TLC-Direct Bioautography Method for Detection of Antiurolithiatic Metabolites." Journal of Chromatographic Science 55, no. 5 (2017): 578–85. http://dx.doi.org/10.1093/chromsci/bmx002.

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9

Choma, I. M. "Thin‐Layer Chromatography‐Direct Bioautography of Flumequine Residues in Milk." Journal of Liquid Chromatography & Related Technologies 29, no. 14 (2006): 2083–93. http://dx.doi.org/10.1080/10826070600759942.

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10

Choma, Irena, Adam Choma, and Krystyna Staszczuk. "Direct bioautography-thin-layer chromatography of flumequine and doxycycline in milk." Journal of Planar Chromatography – Modern TLC 15, no. 3 (2002): 187–91. http://dx.doi.org/10.1556/jpc.15.2002.3.5.

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11

Tock, Margaux Lim Ah, Sandra Combrinck, Guy Kamatou, Weiyang Chen, Sandy Van Vuuren, and Alvaro Viljoen. "Antibacterial Screening, Biochemometric and Bioautographic Evaluation of the Non-Volatile Bioactive Components of Three Indigenous South African Salvia Species." Antibiotics 11, no. 7 (2022): 901. http://dx.doi.org/10.3390/antibiotics11070901.

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Salvia africana-lutea L., S. lanceolata L., and S. chamelaeagnea L. are used in South Africa as traditional medicines to treat infections. This paper describes an in-depth investigation into their antibacterial activities to identify bioactive compounds. Methanol extracts from 81 samples were screened against seven bacterial pathogens, using the microdilution assay. Biochemometric models were constructed using data derived from minimum inhibitory concentration (MIC) and ultra-performance liquid chromatography-mass spectrometry data. Active molecules in selected extracts were tentatively identi
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12

Balázs, Viktória Lilla, Barbara Horváth, Erika Kerekes, et al. "Anti-Haemophilus Activity of Selected Essential Oils Detected by TLC-Direct Bioautography and Biofilm Inhibition." Molecules 24, no. 18 (2019): 3301. http://dx.doi.org/10.3390/molecules24183301.

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Essential oils (EOs) are becoming increasingly popular in medical applications because of their antimicrobial effect. Direct bioautography (DB) combined with thin layer chromatography (TLC) is a screening method for the detection of antimicrobial compounds in plant extracts, for example, in EOs. Due to their lipophilic character, the common microbiological assays (etc. disk diffusion) could not provide reliable results. The aim of this study was the evaluation of antibacterial and anti-biofilm properties of the EO of cinnamon bark, clove, peppermint, thyme, and their main components against Ha
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13

Choma, I. M., A. Choma, I. Komaniecka, K. Pilorz, and K. Staszczuk. "Semiquantitative Estimation of Enrofloxacin and Ciprofloxacin by Thin‐Layer Chromatography–Direct Bioautography." Journal of Liquid Chromatography & Related Technologies 27, no. 13 (2004): 2071–85. http://dx.doi.org/10.1081/jlc-120039419.

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14

Wanschura, Regina, Matthias Baumgartner, Claudia U. Linder, Elisabeth Windeisen, J. Philipp Benz, and Klaus Richter. "Direct bioautography for the screening of selected tropical wood extracts against basidiomycetes." Holzforschung 74, no. 8 (2020): 733–43. http://dx.doi.org/10.1515/hf-2019-0153.

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AbstractTo understand the reasons for the high durability of tropical wood species, the chemistry of the extractives needs to be elucidated. As these extractives consist of a great variety of components differing in quantity and composition, the analysis is often time-consuming. To focus on the key bioactive substances, bioassay-guided fractionation is helpful, but the established bioassay methods cannot be readily adapted to basidiomycete fungi that are commonly used for the respective durability tests, because they do not sporulate easily in laboratory settings. The research therefore aims a
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15

Grzelak, Edyta M., Barbara Majer-Dziedzic, and Irena M. Choma. "Development of a Novel Direct Bioautography–Thin-Layer Chromatography Test: Optimization of Growth Conditions for Gram-Negative Bacteria, Escherichia coli." Journal of AOAC INTERNATIONAL 94, no. 5 (2011): 1567–72. http://dx.doi.org/10.5740/jaoac.10-385.

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Abstract With the aim of developing a TLC-direct bioautography assay using Escherichia coli as test bacteria, various parameters influencing the viability of microorganisms on TLC plates were examined and checked for flumequine standards. The optimal times for preincubation and incubation of bacterial broth were 20 h at 37°C and 2 h at 37°C, respectively. The optimal viscosity of the broth was obtained for 0.05% agarose solution in Mueller-Hinton broth. Various incubation times of the seeded TLC plates were also tested (5 h proved to be optimal). After incubation, the plates were sprayed with
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16

Shafique, Irsa, Saiqa Andleeb, Shaukat Ali, Rozina Ghulam Mustafa, and Anum Naseer. "In Vitro Qualitative Phytochemical Screening, Tlc-Bioautography and Spot Screening of Bistorta Amplexicaulis (D.DON) Greene Extracts." Bangladesh Journal of Botany 50, no. 3 (2021): 613–22. http://dx.doi.org/10.3329/bjb.v50i3.55841.

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The biological activities of Bistorta amplexicaulis (D.Don) Greene rhizome and leaves extracts were evaluated. In vitro antibacterial activity, direct bioautography, and spot screening of TLC developed bands were investigated against seven bacterial pathogens. Screening of phytochemical constituents was also done through both qualitative and thin layer chromatographic methods. DMSO extracts of rhizome indicated high sensitivity (12.33 ± 1.52 and 11.33 ± 0.57 mm) against Escherichia coli. Whereas methanolic and acetonic extracts of rhizome indicated significant inhibition (11.66 ± 1.15 and 11.3
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17

Horváth, Gy, L. Botz, B. Kocsis, É. Lemberkovics, and L. Gy Szabó. "Antimicrobial natural products and antibiotics detected by direct bioautography using plant pathogenic bacteria." Acta Botanica Hungarica 46, no. 1-2 (2004): 153–65. http://dx.doi.org/10.1556/abot.46.2004.1-2.11.

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18

Orłowska, Marta, Teresa Kowalska, Mieczysław Sajewicz, et al. "A Comparison of Antibacterial Activity of Selected Thyme (Thymus) Species by Means of the Dot Blot Test with Direct Bioautographic Detection." Journal of AOAC INTERNATIONAL 98, no. 4 (2015): 871–75. http://dx.doi.org/10.5740/jaoacint.sge5-orlowska.

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Abstract Bioautography carried out with the aid of thin-layer chromatographic adsorbents can be used to assess antibacterial activity in samples of different origin. It can either be used as a simple and cost-effective detection method applied to a developed chromatogram, or to the dot blot test performed on a chromatographic plate, where total antibacterial activity of a sample is scrutinized. It was an aim of this study to compare antibacterial activity of 18 thyme (Thymus) specimens and species (originating from the same gardening plot and harvested in the same period of time) by means of a
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19

Jesionek, Wioleta, Edyta Grzelak, Barbara Majer-Dziedzic, and Irena Choma. "Thin-layer chromatography — Direct bioautography for the screening of antimicrobial properties of plant extracts." Journal of Planar Chromatography – Modern TLC 26, no. 2 (2013): 109–13. http://dx.doi.org/10.1556/jpc.26.2013.2.1.

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Choma, Irena, and Wioleta Jesionek. "TLC-Direct Bioautography as a High Throughput Method for Detection of Antimicrobials in Plants." Chromatography 2, no. 2 (2015): 225–38. http://dx.doi.org/10.3390/chromatography2020225.

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21

Guleria, Sanjay, Ashok Kumar, and Ashok Kumar Tiku. "Chemical Composition and Fungitoxic Activity of Essential Oil of Thuja orientalis L. Grown in the North-Western Himalaya." Zeitschrift für Naturforschung C 63, no. 3-4 (2008): 211–14. http://dx.doi.org/10.1515/znc-2008-3-409.

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The essential oil from fresh leaves of Thuja orientalis L. grown in the north-western Himalaya was isolated by means of hydrodistillation and analyzed by GC and GC/MS. Twentytwo compounds representing 94.0% of the total oil were identified. The leaf oil contained α- pinene (29.2%), Δ-3-carene (20.1%), α-cedrol (9.8%), caryophyllene (7.5%), α-humulene (5.6%), limonene (5.4%), α-terpinolene (3.8%) and α-terpinyl acetate (3.5%) as major constituents. The essential oil showed antifungal activity against Alternaria alternata in a direct bioautography assay. Two main bioactive compounds named as b1
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22

Rakotoniriana, Erick Francisco, Gabrielle Chataigné, Guy Raoelison, et al. "Characterization of an endophytic whorl-forming Streptomyces from Catharanthus roseus stems producing polyene macrolide antibiotic." Canadian Journal of Microbiology 58, no. 5 (2012): 617–27. http://dx.doi.org/10.1139/w2012-034.

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An endophytic whorl-forming Streptomyces sp. designated as TS3RO having antifungal activity against a large number of fungal pathogens, including Sclerotinia sclerotiorum , Rhizoctonia solani , Colletotrichum gloeosporioides , Cryphonectria parasitica , Fusarium oxysporum , Pyrenophora tritici-repentis , Epidermophyton floccosum , and Trichophyton rubrum , was isolated from surface-sterilized Catharanthus roseus stems. Preliminary identification showed that Streptomyces cinnamoneus subsp. sparsus was its closest related species. However, strain TS3RO could readily be distinguished from this sp
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23

Sherma, Joseph. "Review of the Determination of the Antioxidant Activity of Foods, Food Ingredients, and Dietary Supplements by Thin Layer Chromatography-Direct Bioautography, Spectrometry, and the Dot-Blot Procedure." Journal of AOAC INTERNATIONAL 101, no. 5 (2018): 1285–94. http://dx.doi.org/10.5740/jaoacint.18-0116.

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Abstract This paper reviews a selection of the most important studies on antioxidants in foods (including beverages), food ingredients, and dietary supplements by effect-directed analysis using TLC with DPPH*, ABTS*+, and β-carotene direct bioautography. Total antioxidant activity by visible mode spectrometry (colorimetry) with TLC used offline to obtain additional analytical results, mostly for identification and quantification of phenolic compounds in samples, is also discussed. Finally, dot-blot assay for total antioxidant activity, carried out on a TLC plate without mobile-phase developmen
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dos Santos, Ruilan Alves, Clécio Souza Ramos, Maria Cláudia M. Young, et al. "Antifungal Constituents from the Roots ofPiper dilatatumRich." Journal of Chemistry 2013 (2013): 1–5. http://dx.doi.org/10.1155/2013/160165.

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The compounds (+)-(7S,8R)-epoxy-5,6-didehydrokavain (1), flavokavain B (2),β-sitosterol (3), and stigmasterol (4) are reported here as chemical constituents ofPiper dilatatumRich. (Piperaceae). Their structures were determined on the basis of their spectroscopic data (1H and13C NMR, MS, and IR). The antifungal activities of pyrone1(1 μg) and chalcone2(100 μg) were determined by means of direct bioautography againstCladosporium cladosporioidesandC. sphaerospermum. Results indicateP. dilatatumas a candidate for the development of novel antifungal phytotherapic products as well as point out pyron
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25

Móricz, Ágnes M., Péter G. Ott, Ágnes Alberti, et al. "Applicability of Preparative Overpressured Layer Chromatography and Direct Bioautography in Search of Antibacterial Chamomile Compounds." Journal of AOAC INTERNATIONAL 96, no. 6 (2013): 1214–21. http://dx.doi.org/10.5740/jaoacint.sgemoricz.

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Abstract In situ sample preparation and preparative overpressured layer chromatography (OPLC) fractionation on a 0.5 mm thick adsorbent layer of chamomile flower methanol extract prepurified by conventional gravitation accelerated column chromatography were applied in searching for bioactive components. Sample cleanup in situ on the adsorbent layer subsequent to sample application was performed using mobile phase flow in the opposite direction (the input and output of the eluent was exchanged). The antibacterial effect of the fractions obtained from the stepwise gradient OPLC separation with t
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Móricz, Ágnes, Szabolcs Szarka, Péter Ott, et al. "Application of direct bioautography and SPME-GC-MS for the study of antibacterial chamomile ingredients." Journal of Planar Chromatography – Modern TLC 25, no. 3 (2012): 220–24. http://dx.doi.org/10.1556/jpc.25.2012.3.6.

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Klingelhöfer, Ines, and Gertrud E. Morlock. "Bioprofiling of Surface/Wastewater and Bioquantitation of Discovered Endocrine-Active Compounds by Streamlined Direct Bioautography." Analytical Chemistry 87, no. 21 (2015): 11098–104. http://dx.doi.org/10.1021/acs.analchem.5b03233.

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Grzelak, Edyta M., Barbara Majer-Dziedzic, Irena M. Choma, and Karol M. Pilorz. "Development of a Novel Direct Bioautography-Thin-Layer Chromatography Test: Optimization of Growth Conditions for Gram-Positive Bacteria, Bacillus subtilis." Journal of AOAC INTERNATIONAL 96, no. 2 (2013): 386–91. http://dx.doi.org/10.5740/jaoacint.11-466.

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Abstract A TLC-direct bioautography (DB) assay using Bacillus subtilis as test bacteria was developed. Various factors affecting the microorganism's viability on the TLC plates were studied and verified for the flumequine standards. The Dhenasar's method called "direct sample determination" was used for TLC; the antibiotic samples were spotted on the TLC plates and subjected to bioautography without developing with a mobile phase. The best preincubation and incubation times of bacterial broth were found to be 1 h at 37°C and 6 h at 37°C. The optimal viscosity of broth was obtained by the addit
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Móricz, Ágnes M., Györgyi Horváth, Andrea Böszörményi, and Péter G. Ott. "Detection and Identification of Antibacterial and Antioxidant Components of Essential Oils by TLC-Biodetection and GC-MS." Natural Product Communications 11, no. 11 (2016): 1934578X1601101. http://dx.doi.org/10.1177/1934578x1601101120.

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Components of cinnamon bark, rosemary, clove and thyme essential oils were screened for antioxidant and antibacterial activity utilizing thin-layer chromatography (TLC) coupled with the DPPH• test and direct bioautography using Bacillus subtilis cells. The compounds in the active chromatographic zones were identified by solid-phase microextraction-gas chromatography-mass spectrometry (SPME-GC-MS) after their elution. Seven antibacterial components were found: cinnamaldehyde and eugenol in cinnamon bark oil, 1,8-cineole, camphor, borneol and α-terpineol in rosemary oil, eugenol in clove oil and
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Mincsovics, Emil, and Ernő Tyihák. "Overpressured Layer Chromatography (OPLC) – A Flexible Tool of Analysis and Isolation." Natural Product Communications 6, no. 5 (2011): 1934578X1100600. http://dx.doi.org/10.1177/1934578x1100600528.

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This review briefly summarizes the overpressured layer chromatography (OPLC) technique and its progress from the beginning until today. Some theoretical aspects, important technical and methodological solutions, as well as analytical separations and isolations are demonstrated covering the last 30 years. The infusion and transfusion OPLC operations, as well as their combination, and their off-line and online processes, and off-line and online hyphenations for detection and structure elucidation are presented. The combination of OPLC separation with biological detection by direct bioautography
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Isnaeni, Isnaeni, Andri Astuti, and Muhammad Yuwono. "Validation of Thin-Layer Chromatography-Bioautographic Method for Determination of Streptomycin." JURNAL FARMASI DAN ILMU KEFARMASIAN INDONESIA 4, no. 1 (2018): 34. http://dx.doi.org/10.20473/jfiki.v4i12017.34-38.

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Background: A simple bio-assay for determination of streptomycin hyphenated with planar chromatography techniques was developed. Objective: This study aims to validate the method for identification and determination of streptomycin in injection preparations with TLC-bioautography. Methods: Thin Layer Chromatography (TLC) was performed on the silica Gel GF-254 using KH2PO4 solution as mobile solvent. The visualization was performed by spraying 2% resorcinol. Direct bi autography was developed using Escherichia coli ATCC 25922 as a bacterial test, grown on the nutrient agar medium at 37oC for 24
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Yüce, Imanuel, and Gertrud E. Morlock. "Nanomole-scaled high-throughput chemistry plus direct bioautography on the same chromatography plate for drug discovery." Analytica Chimica Acta 1182 (October 2021): 338950. http://dx.doi.org/10.1016/j.aca.2021.338950.

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Choma, Irena. "Screening of enrofloxacin and ciprofloxacin residues in milk by HPLC and by TLC with direct bioautography." Journal of Planar Chromatography – Modern TLC 19, no. 108 (2006): 104–8. http://dx.doi.org/10.1556/jpc.19.2006.2.3.

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Jesionek, Wioleta, Irena Maria Choma, Barbara Majer-Dziedzic, and Irena Malinowska. "SCREENING BACTERIAL AND RADICAL SCAVENGING PROPERTIES OF CHOSEN PLANT EXTRACTS USING THIN-LAYER CHROMATOGRAPHY–DIRECT BIOAUTOGRAPHY." Journal of Liquid Chromatography & Related Technologies 37, no. 20 (2014): 2882–91. http://dx.doi.org/10.1080/10826076.2014.907103.

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Krüzselyi, Dániel, János Vetter, Péter G. Ott, and Ágnes M. Móricz. "Investigation of antibacterial components of button mushroom (Agaricus bisporus) by direct bioautography and HPLC–DAD–MS." Journal of Liquid Chromatography & Related Technologies 39, no. 5-6 (2016): 298–302. http://dx.doi.org/10.1080/10826076.2016.1163469.

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Kusumawati, Pipin, Yosi Bayu Murti, and Nastiti Wijayanti. "Screening of anti-Candida albicans metabolites produced by marine sponge-associated bacteria." Marine Research in Indonesia 45, no. 2 (2020): 47–58. http://dx.doi.org/10.14203/mri.v45i2.575.

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This study selected bacteria with high anti-Candida albicans (CA) activity among ten bacteria isolated from marine sponges. Bacteria were cultivated using the basal medium to produce the extract. Minimum Inhibitory Concentration (MIC) microdilution broth was used as an anti-CA assay followed by TLC-direct bioautography to characterize their active compound with spray reagents. The bacteria determination was done by molecular approaches using Repetitive-Element Sequences-based-PCR (rep-PCR) and amplification of 16S rDNA partial gene sequences, continued with BLAST analysis. The four out of ten
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Jesionek, Wioleta, Ágnes M. Móricz, Ágnes Alberti, et al. "TLC-Direct Bioautography as a Bioassay Guided Method for Investigation of Antibacterial Compounds in Hypericum perforatum L." Journal of AOAC INTERNATIONAL 98, no. 4 (2015): 1013–20. http://dx.doi.org/10.5740/jaoacint.14-233.

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Abstract Fast high-throughput TLC-direct bioautography (DB) is an effect-directed analysis method that enables searching for biologically active (e.g., antimicrobial) substances in complex mixtures like plant extracts. The principle of the method is that separation and detection of biological properties of given mixture components is performed directly on a TLC plate. In searching for antibacterial activity, the developed plate is immersed in a bacterial broth, and bacteria grow directly on its layer during a proper incubation time. Inhibition zones are formed in places where antimicrobial com
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Jóźwiak, Grzegorz W., Barbara Majer-Dziedzic, Wioleta Jesionek, W. Zieliński, and Monika Waksmundzka-Hajnos. "Thin-layer chromatography: Direct bioautography as a method of examination of antimicrobial activity of selected Potentilla species." Journal of Liquid Chromatography & Related Technologies 39, no. 5-6 (2016): 281–85. http://dx.doi.org/10.1080/10826076.2016.1163466.

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Boulenouar, N., A. Marouf, and A. Cheriti. "EFFECT OF EXTRACTS FROM PHOENIX DACTYLIFERA L. RACHIS ON FUSARIUM OXYSPORUM F. SP. ALBEDINIS USING DIRECT BIOAUTOGRAPHY." Acta Horticulturae, no. 994 (June 2013): 385–88. http://dx.doi.org/10.17660/actahortic.2013.994.40.

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40

Oberle, M., P. Lewits, J. Engemann, I. Klingelhöfer, and G. Morlock. "Modern direct bioautography for fast screening and characterization of active compounds in plant extracts used in cosmetics." Planta Medica 81, S 01 (2016): S1—S381. http://dx.doi.org/10.1055/s-0036-1596279.

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41

Piasecki, Bartłomiej, Anna Biernasiuk, Adrianna Skiba, Krystyna Skalicka-Woźniak, and Agnieszka Ludwiczuk. "Composition, Anti-MRSA Activity and Toxicity of Essential Oils from Cymbopogon Species." Molecules 26, no. 24 (2021): 7542. http://dx.doi.org/10.3390/molecules26247542.

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Many of the essential oils obtained from medicinal plants possess proven antimicrobial activity and are suitable for medicinal purposes and applications in the food industry. The aim of the present work was the chemical analysis of 19 essential oils (EOs) from seven different Cymbopogon species (C. nardus, C. citratus, C winterianus, C. flexuosus, C. schoenanthus, C. martinii, C. giganteus). Five different chemotypes were established by GC/MS and TLC assay. The EOs, as well as some reference compounds, i.e., citronellol, geraniol and citral (neral + geranial), were also tested for their antimi
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42

Häbe, Tim T., Maryam Jamshidi-Aidji, Jennifer Macho, and Gertrud E. Morlock. "Direct bioautography hyphenated to direct analysis in real time mass spectrometry: Chromatographic separation, bioassay and mass spectra, all in the same sample run." Journal of Chromatography A 1568 (September 2018): 188–96. http://dx.doi.org/10.1016/j.chroma.2018.07.002.

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43

Stankovic, Jovana, Miroslav Novakovic, Vele Tesevic, et al. "HPTLC-direct bioautography-guided isolation of isogeranic acid as the main antibacterial constituent of Artemisia santonicum essential oil." Journal of the Serbian Chemical Society 84, no. 12 (2019): 1355–65. http://dx.doi.org/10.2298/jsc190513106s.

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This study was performed to determine the main antibacterial compounds of the essential oil (??) of saltmarsh plant Artemisia santonicum (Asteraceae). The combination of HPTLC and direct bioautography was used for the activity guided isolation of isogeranic acid as the main antibacterial constituent with remarkable antimicrobial activity, although it was the minor component of the EO, present only in 0.2 %, as calculated from GC/FID. Its structure was determined by 1D- and 2D-NMR and GC?MS techniques. Antibacterial activity of isogeranic acid against all tested bacteria was significantly highe
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44

Mustafa, Rozina Ghulam, Andleeb Dr Saiqa, Jorge Domínguez, et al. "Therapeutic Values of Earthworm Species Extract from Azad Kashmir as Anticoagulant, Antibacterial, and Antioxidant Agents." Canadian Journal of Infectious Diseases and Medical Microbiology 2022 (February 7, 2022): 1–20. http://dx.doi.org/10.1155/2022/6949117.

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Aims. Current research aimed to explore the therapeutic values of different earthworms as antibacterial, anticoagulant, and antioxidant agents. Methods. Ten different earthworms, i.e., Amynthas corticis, Amynthas gracilis, Pheretima posthuma, Eisenia fetida, Aporrectodea rosea, Allolobophora chlorotica, Aporrectodea trapezoides, Polypheretima elongata, Aporrectodea caliginosa, and Pheretima hawayana, were collected and screened for biological activities. Antibacterial effect analysis of earthworm species was done against fourteen bacterial pathogens, i.e., Escherichia coli, Serratia marcescens
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45

Ramírez-Rueda, Román Yesid, Jane Marinho, and Marcos José Salvador. "Bioguided identification of antimicrobial compounds from Chrysopogon zizaniodes (L.) Roberty root essential oil." Future Microbiology 14, no. 14 (2019): 1179–89. http://dx.doi.org/10.2217/fmb-2019-0167.

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Aim: To determine the group of compounds from Chrysopogon zizaniodes root essential oil that have antimicrobial activity. Materials & methods: Thin-layer chromatography coupled to direct bioautography was used to determinate the fraction(s) having antimicrobial activity against methicillin-resistant Staphylococcus aureus (MRSA) and vancomycin-resistant Enterococcus faecalis (VREF). Through GC-MS identification, the fractions with the greatest similarity to the active thin-layer chromatography fraction were used to determinate the MIC. Results: The subfraction 8 from column chromatography w
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46

Tabanca, Nurhayat, David E. Wedge, Xiaoning Wang, et al. "Chemical Composition and Antifungal Activity of Angelica sinensis Essential Oil Against three Colletotrichum Species." Natural Product Communications 3, no. 7 (2008): 1934578X0800300. http://dx.doi.org/10.1177/1934578x0800300708.

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Chemical fungicides are an important component in disease management for most crops. As part of a program to discover natural product-based fungicides, several sensitive assay systems have been developed for the evaluation of naturally occurring antifungal agents. In this study, we focused on the discovery and evaluation of new natural product-based fungicides from Angelica sinensis (Dong Quai) for use in control of strawberry anthracnose caused by Colletotrichum species. A. sinensis oil was evaluated for antifungal activity against three Colletotrichum species using direct-bioautography assay
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47

Pyka, Alina. "Detection Progress of Selected Drugs in TLC." BioMed Research International 2014 (2014): 1–19. http://dx.doi.org/10.1155/2014/732078.

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This entry describes applications of known indicators and dyes as new visualizing reagents and various visualizing systems as well as photocatalytic reactions and bioautography method for the detection of bioactive compounds including drugs and compounds isolated from herbal extracts. Broadening index, detection index, characteristics of densitometric band, modified contrast index, limit of detection, densitometric visualizing index, and linearity range of detected compounds were used for the evaluation of visualizing effects of applied visualizing reagents. It was shown that visualizing effec
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48

Piech, Tomasz, Barbara Majer-Dziedzic, Anna Kostruba, Edyta M. Grzelak, and Irena M. Choma. "Thin-layer chromatography—direct bioautography as an alternative method for screening of antibiotic residues in milk: A comparative study." Journal of Liquid Chromatography & Related Technologies 39, no. 5-6 (2016): 292–97. http://dx.doi.org/10.1080/10826076.2016.1163468.

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49

Jóźwiak, Grzegorz, Barbara Majer-Dziedzic, Justyna Kwiecińska, and Monika Waksmundzka-Hajnos. "Comparison of the microbiological activities of different varieties of hop (Humulus lupulus) extracts by thin-layer chromatography—direct bioautography." JPC - Journal of Planar Chromatography - Modern TLC 30, no. 2 (2017): 126–30. http://dx.doi.org/10.1556/1006.2017.30.2.6.

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50

Móricz, Ágnes M., Tim T. Häbe, Péter G. Ott, and Gertrud E. Morlock. "Comparison of high-performance thin-layer with overpressured layer chromatography combined with direct bioautography and direct analysis in real time mass spectrometry for tansy root." Journal of Chromatography A 1603 (October 2019): 355–60. http://dx.doi.org/10.1016/j.chroma.2019.03.068.

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