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Tesis sobre el tema "DNA"

Autor: Grafiati

Publicado: 4 de junio de 2021

Última modificación: 25 de julio de 2025

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1

Lo, Allen Tak Yiu. "Protein dynamics on the lagging strand during DNA synthesis." Thesis, School of Chemistry, 2012. https://ro.uow.edu.au/theses/3684.

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DNA replication is one of the vital processes in the cell; it duplicates chromosomal DNA before a cell divides. In all organisms, DNA synthesis on the leading-strand template occurs continuously, whereas on the lagging strand a different mechanism is required. Due to the anti-parallel structure of double-stranded DNA, lagging-strand synthesis requires repeated RNA priming by a specialist primase and synthesis of short Okazaki fragments. How proteins carry out this dynamic process is still unknown. For Escherichia coli DNA replication, a lagging-strand three-point switch was proposed in 1999 to

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2

Martin, Eleyna. "Initiation of DNA replication in Bacillus subtilis : structural studies of the DnaA-DnaD interaction." Thesis, University of Nottingham, 2018. http://eprints.nottingham.ac.uk/53443/.

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Replication of genetic information is a vital process across all domains of life. Bacillus subtilis is considered the gram-positive model bacterium for studying DNA replication (Escherichia coli has been studied extensively as the gram-negative model) and is most representative of the ancestral phylum of prokaryotes. DNA replication has three distinct stages; initiation, elongation and termination. Replication initiation is the focus of this research and this process occurs at a single origin conserved throughout bacteria, termed oriC. B. subtilis primosomal machinery is formed of replication

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3

Komori, Hirofumi. "Structural studies on DNA-binding proteins : DNA replication initiator and DNA photolyase." 京都大学 (Kyoto University), 2002. http://hdl.handle.net/2433/150005.

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4

Bandholtz, Lisa Charlotta. "DNA vaccines and bacterial DNA in immunity /." Stockholm, 2002. http://diss.kib.ki.se/2002/91-7349-340-6/.

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5

Richardson, James Alistair. "Novel DNA probes for sensitive DNA detection." Thesis, University of Southampton, 2010. https://eprints.soton.ac.uk/173981/.

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The ability to detect and interrogate DNA sequences allows further understanding and diagnosis of genetic disease. The ability to perform such analysis of genetic material requires highly selective and reliable technologies. Furthermore techniques which can use simple and cheap equipment allow the use of such technologies for point of care analysis. Described in this thesis are two novel DNA probe systems designed for mutation discrimination and sequence recognition of PCR products. A homogenous PCR system using HyBeacons® which utilise FRET to produce a three probe multiplex system and surfac

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6

Boal, Amie Kathleen Parker Carl Stevens Barton Jacqueline K. "DNA-mediated charge transport in DNA repair /." Diss., Pasadena, Calif. : California Institute of Technology, 2008. http://resolver.caltech.edu/CaltechETD:etd-06022008-092549.

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7

Lo, Pik Kwan Peggy. "Supramolecular DNA chemistry: assembly of DNA nanotubes and templated synthesis of DNA-mimetic polymers." Thesis, McGill University, 2010. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=95152.

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DNA has emerged as a promising template for the programmable assembly of structures on the nanometer scale. In particular, DNA nanotubes hold promise for a number of biological and materials applications, because of their high aspect ratio and encapsulation potential. Current construction methods result in symmetrical and cylindrical assemblies that are totally double-stranded, and are long and polydisperse. In order to investigate DNA nanotubes for applications as well-defined molecular hosts and one-dimensional templates, better control over their geometry, stiffness and porosity, ability to

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8

Araki, Kasumi. "Dual roles for DNA polymerase η in homologous DNA recombination and translesion DNA synthesis". Kyoto University, 2006. http://hdl.handle.net/2433/143860.

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9

CRISTOFALO, MATTEO. "Nanomechanics of DNA and DNA-ligand interactions: focus on structural polymorphism and DNA condensation." Doctoral thesis, Università degli Studi di Milano-Bicocca, 2019. http://hdl.handle.net/10281/241313.

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In the last few decades, the constant development of novel microscopy techniques have created the basis for a new paradigm in the field of biophysics. Single-molecule techniques enabled to carry out experiments providing new information: the nanomanipulation of individual biomolecules revealed unknown insights into the elasticity and mechanics of molecules, improving the understanding of the fundamental relation between structural properties and biological functions. In particular, an AFM and mostly a MT setup were used during this thesis work, both located in biophysics laboratory of Prof.

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10

Grundström, Malin. "DNA : Att tillvarata DNA på ett rättssäkert sätt." Thesis, Umeå University, Basic training programme for Police Officers, 2004. http://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-27188.

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<p>Syftet med arbetet är att belysa problematiken kring DNA som ett bevisinstrument, hur de olika analysformerna går till samt att påvisa vikten av att även polispersonal i yttre tjänst förstår hur och varför det är så viktigt att det tillvaratas på rätt sätt. Det finns mycket litteratur om DNA och jag har försökt begränsa mitt material till de delar som kan intressera poliser. Jag har även försökt att förklara DNA på ett så lätt sätt som möjligt för att den som inte är så bevandrad i biologi skall kunna förstå ett ändå mycket komplext ämne. Företrädelsevis har jag använt mig av skriftlig lit

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11

Pearson, Christopher Edmund. "DNA cruciforms and mammalian origins of DNA replication." Thesis, McGill University, 1994. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=28503.

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The objective of the research in this thesis is to investigate, at the molecular level, the sequences and/or structures involved in the initiation of mammalian DNA replication and to investigate the protein interactions with DNA cruciforms which have been implicated in the process of replication initiation. Four plasmids containing monkey (CV-1) early replicating nascent origin enriched sequences (ors), which had been shown previously to replicate autonomously in transfected CV-1, COS-7 and HeLa cells, were used in the establishment of an in vitro DNA replication system that uses HeLa cell ext

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12

Morant, Nick. "Novel thermostable DNA polymerases for isothermal DNA amplification." Thesis, University of Bath, 2015. https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.667735.

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DNA polymerases play a fundamental role in the transmission and maintenance of genetic information and have become an important in vitro diagnostic and analytical tool. The Loop-mediated isothermal DNA amplification (LAMP) method has major applications for disease and pathogen detection and utilises the unique strand-displacement activity of a small group of thermostable DNA polymerases. The Large (Klenow-like) Fragment of Geobacillus stearothermophilus DNA polymerase I (B.st LF Pol I) currently serves as the enzyme of choice for the majority of these isothermal reactions, with few alternative

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13

Carson, Christian Tyler. "DNA viruses and the cellular DNA repair machinery /." Diss., Connect to a 24 p. preview or request complete full text in PDF format. Access restricted to UC campuses, 2005. http://wwwlib.umi.com/cr/ucsd/fullcit?p3175282.

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14

Leavitt, Markley Carl. "Bacteriophage T5 DNA polymerase relationships of DNA polymerases." Diss., The University of Arizona, 1990. http://hdl.handle.net/10150/185335.

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T5 DNA polymerase, a highly processive single polypeptide enzyme, and PRD1 DNA polymerase, a protein-primed DNA polymerase, have been analyzed for their primary structural features. The amino acid sequence of T5 DNA polymerase reveals a high degree of homology with DNA polymerase I (Pol I) of Escherichia coli and retains many of the amino acid residues which have been implicated in the 3'-5' exonuclease and DNA polymerase activities of that enzyme. Alignment with sequences of polymerase I and T7 DNA polymerase (family A polymerases) was used to identify regions possibly involved in the high pr

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15

Maul, Melanie. "DNA Photolyasen." Diss., lmu, 2009. http://nbn-resolving.de/urn:nbn:de:bvb:19-109219.

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16

Günthner-Biller, Maria Margarete. "Immunstimulatorische DNA." Diss., lmu, 2011. http://nbn-resolving.de/urn:nbn:de:bvb:19-128724.

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17

Mitchell, James Christopher. "DNA nanostructures." Thesis, University of Oxford, 2003. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.400206.

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18

Goodman, Russell Paul. "DNA tetrahedra." Thesis, University of Oxford, 2006. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.433479.

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19

Green, Simon. "DNA nanomachines." Thesis, University of Oxford, 2006. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.432563.

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20

Amos, Martyn. "DNA computation." Thesis, University of Warwick, 1997. http://wrap.warwick.ac.uk/4238/.

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This is the first ever doctoral thesis in the field of DNA computation. The field has its roots in the late 1950s, when the Nobel laureate Richard Feynman first introduced the concept of computing at a molecular level. Feynman's visionary idea was only realised in 1994, when Leonard Adleman performed the first ever truly molecular-level computation using DNA combined with the tools and techniques of molecular biology. Since Adleman reported the results of his seminal experiment, there has been a flurry of interest in the idea of using DNA to perform computations. The potential benefits of usin

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21

Chang, Hung Yuan. "Bridge DNA." Thesis, Rochester Institute of Technology, 2018. http://pqdtopen.proquest.com/#viewpdf?dispub=10822889.

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<p> This thesis work centers on the relationship between bridge structure, construction methods and timberwork, coming from the processes of design and manufacturing. In this thesis, I intend to discover and develop different characteristics of different structures and materials and create works that are visually powerful using different visual languages. I began to learn about bridge design after I started working full time after graduating from university. During that period I was involved in the creation of several bridge designs and obtained experience as a builder. The bridge that I helpe

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22

Zwolak, Michael Philip. "DNA Electronics." Thesis, Virginia Tech, 2003. http://hdl.handle.net/10919/78135.

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DNA is a potential component in molecular electronics. To explore this end, there has been an incredible amount of research on how well DNA conducts and by what mechanism. There has also been a tremendous amount of research to find new uses for it in nanoscale electronics. DNA's self-assembly and recognition properties have found a unique place in this area. We predict, using a tight-binding model, that spin-dependent transport can be observed in short DNA molecules sandwiched between ferromagnetic contacts. In particular, we show that a DNA spin-valve can be realized with magnetoresistance va

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23

Aparício, James Monteiro. "DNA symmetry." Master's thesis, Universidade de Aveiro, 2011. http://hdl.handle.net/10773/8627.

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Mestrado em Engenharia de Computadores e Telemática<br>A investigação do DNA tem sido uma das áreas de investigação mais exploradas no último século. Desde a sua primeira descrição até à primeira sequência completa do genoma humano muito foi descoberto, mas ainda estamos longe de o compreender completamente. Neste trabalho tentámos explorar a ordem até à qual se verifica a existência de simetria relevante em genomas, e para esse fim, usámos um conjunto de genomas de vários organismos. Tentámos encontar relação entre os vários genomas através das características de simetria. Foram analisados

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24

Nucci, André Luis Carrilho. "DNA arquitetônico." reponame:Repositório Institucional da UFSC, 2012. http://repositorio.ufsc.br/xmlui/handle/123456789/100891.

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Dissertação (mestrado) - Universidade Federal de Santa Catarina, Centro Tecnológico, Programa de Pós-graduação em Arquitetura e Urbanismo<br>Made available in DSpace on 2013-06-25T23:38:38Z (GMT). No. of bitstreams: 1 313280.pdf: 11205896 bytes, checksum: 0930b6dcc0221ca4f7b13aed4dddc372 (MD5)<br>Ao longo do tempo se produziu um estoque de artefatos arquitetônicos, e para a preservação dos mesmos já existem diretrizes, porém ficam aos cuidados dessas, somente poucos exemplares responsáveis por manter a memoria cultural, mais do que ser essa representação histórica, a arquitetura deve cumprir u

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25

Pospiech, H. (Helmut). "The role of DNA polymerases, in particular DNA polymerase ε in DNA repair and replication". Doctoral thesis, University of Oulu, 2002. http://urn.fi/urn:isbn:9514266692.

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Abstract Analysis of the primary structure of DNA polymerase ε B subunit defined similarities to B subunits of eukaryotic DNA polymerases α, δ and ε as well as the small subunits of DNA polymerase DI of Euryarchaeota. Multiple sequence alignment of these proteins revealed the presence of 12 conserved motifs and defined a novel protein superfamily. The members of the B subunit family share a common domain architecture, suggesting a similar fold, and arguing for a conserved function among these proteins. The contribution of human DNA polymerase ε to nuclear DNA replication was studied u

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26

Boccongelli, Marina. "Etude expérimentale de la stabilité, sélectivité d'appariement et dynamique d'oligonucléotides DNA-DNA et LNA-DNA." Doctoral thesis, Universite Libre de Bruxelles, 2008. http://hdl.handle.net/2013/ULB-DIPOT:oai:dipot.ulb.ac.be:2013/210549.

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Le traitement et le diagnostic de maladies d'origine génétique suscite un grand intérêt à l'heure actuelle. De par leur spécificité d'appariement avec les acides nucléiques, les oligonucléotides possèdent un grand potentiel dans ce domaine. Ils se heurtent toutefois à des limitations majeures, dont leur faible stabilité en milieu physiologique et la difficulté qu'ils ont à franchir les membranes biologiques. De nombreuses équipes de recherche s'intéressent, afin de pallier ces limitations, à la conception et à la synthèse d'oligonucléotides chimiquement modifiés. Parmi ceux-ci, les Locked Nucl

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27

Aldaye, Faisal A. 1979. "Supramolecular DNA nanotechnology : discrete nanoparticle organization, three-dimensional DNA construction, and molecule templated DNA assembly." Thesis, McGill University, 2008. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=115668.

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The field of structural DNA nanotechnology utilizes DNA's powerful base-pairing molecular recognition criteria to help solve real challenges facing researchers in material science and nanotechnology, some of which include synthesis, sensing, catalysis, delivery, storage, optics, electronics, and scaffolding. In it, DNA is stripped away from any of its preconceived biological roles, and is treated as a powerful synthetic polymer. A subarea of research that our group has recently termed supramolecular DNA nanotechnology is emerging, and is proving to be a powerful complement to some of the alrea

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28

Zhang, Jianhua. "Restriction fragment length polymorphism analysis of chloroplast DNA, mitochondrial DNA, and ribosomal DNA in turfgrasses." Diss., This resource online, 1994. http://scholar.lib.vt.edu/theses/available/etd-06062008-170748/.

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29

Rebelo, Adriana. "Probing Mitochondrial DNA Structure with Mitochondria-Targeted DNA Methyltransferases." Scholarly Repository, 2009. http://scholarlyrepository.miami.edu/oa_dissertations/344.

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The mitochondria contain their own genome, which is organized in a dynamic high-order nucleoid structure consisting of several copies of mitochondrial DNA (mtDNA) molecules associated with proteins. The mitochondrial nucleoids are the units of mtDNA inheritance, and are the sites of mtDNA transcription, replication and maintenance. Therefore, the integrity of mitochondrial nucleoids is a key determinant of mitochondrial metabolism and the bioenergetic state of the cell. Deciphering the interaction of mtDNA with proteins in nucleoprotein complexes is fundamental to understand the mechanisms of

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30

Sando, Shinsuke. "RATIONAL DESIGN OF DNA-BINDING MOLECULES AND DNA PHOTOCLEAVERS." 京都大学 (Kyoto University), 2001. http://hdl.handle.net/2433/150700.

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31

Protozanova, Ekaterina. "Physicochemical characterization of multistranded DNA assemblies, DNA frayed wires." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 2000. http://www.collectionscanada.ca/obj/s4/f2/dsk3/ftp04/nq53831.pdf.

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32

Peeva, Viktoriya [Verfasser]. "Rearranged DNA in mitochondrial DNA maintenance disorders / Viktoriya Peeva." Bonn : Universitäts- und Landesbibliothek Bonn, 2015. http://d-nb.info/1077289669/34.

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33

Rungsardthong, Uracha. "Physicochemical evaluation of polymer-DNA complexes for DNA delivery." Thesis, University of Nottingham, 2002. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.394855.

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34

Lever, Catherine. "The study of DNA-DNA interactions using modified oligonucleotides." Thesis, University of Liverpool, 1993. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.317140.

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35

Mackenney, Victoria Jane. "Human DNA ligase I in DNA replication and repair." Thesis, King's College London (University of London), 1997. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.267515.

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36

Minchell, Nicola E. "DNA topological stress during DNA replication in Saccharomyces cerevisiae." Thesis, University of Sussex, 2019. http://sro.sussex.ac.uk/id/eprint/81222/.

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DNA topological stress impedes normal DNA replication. If topological stress is allowed to build up in front of the replication fork, the fork rotates to overcome the stress, leading to formation of DNA pre-catenanes. The formation of DNA pre-catenanes is therefore a marker of DNA topological stress. In this study, I have examined how transcription linked DNA topological stress impacts on fork rotation and on endogenous DNA damage. Transcription, similar to replication, affects the topology of the DNA; and collision between the two machineries is likely to lead to high levels of DNA topologica

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37

Gould, Poppy Aeron. "The role of DNA repair in DNA methylation dynamics." Thesis, University of Cambridge, 2018. https://www.repository.cam.ac.uk/handle/1810/274360.

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The mammalian epigenome is globally reprogrammed at two stages of development; this involves the erasure and re-establishment of DNA methylation by both passive and active mechanisms, including DNA repair pathways, and occurs concurrently with an increase in developmental potency. In addition to Uhrf1 and the Tet enzymes, the interplay between activation induced cytidine deaminase (AID) and the DNA repair machinery has been implicated in epigenetic reprogramming of various in vivo and in vitro systems including mouse primordial germ cells, zygotes and induced pluripotent stem cells. AID deamin

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38

Neaves, Kelly Jane. "Atomic force microscopy of DNA and DNA-protein constructs." Thesis, University of Cambridge, 2010. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.608615.

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39

Palm, Nathalie. "DNA-bevisning : om värdering av DNA-spår i brottmål." Thesis, Stockholms universitet, Juridiska institutionen, 2016. http://urn.kb.se/resolve?urn=urn:nbn:se:su:diva-127740.

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40

Ouldridge, Thomas E. "Coarse-grained modelling of DNA and DNA self-assembly." Thesis, University of Oxford, 2011. http://ora.ox.ac.uk/objects/uuid:b2415bb2-7975-4f59-b5e2-8c022b4a3719.

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In this thesis I present a novel coarse-grained model of deoxyribonucleic acid (DNA). The model represents single-stranded DNA as a chain of rigid nucleotides, and includes potentials to represent chain connectivity, excluded volume, hydrogen-bonding and base stacking interactions. The parameterization of these interactions is justified by comparing the model's representation of a range of physical phenomena to experimental data. In particular, the geometrical structure and elastic moduli of duplex DNA, and the flexibility of single-stranded DNA, are shown to be physically reasonable. Addition

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41

Isoz, Isabelle. "Role of yeast DNA polymerase epsilon during DNA replication." Doctoral thesis, Umeå : Umeå University, 2008. http://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-1932.

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42

Punchihewa, Chandanamalie. "DNA and DNA-Interacting Proteins as Anticancer Drug Targets." Diss., The University of Arizona, 2006. http://hdl.handle.net/10150/194379.

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DNA is both the oldest and newest of targets for cancer therapy. While it is already being targeted by many anticancer drugs in the clinic, the development of sequence-specific DNA binders has brought it back to the limelight as a valuable anticancer drug target.My studies on DNA interacting agents was initiated with the DNA intercalator campotothecin, and also included topoisomerase I enzyme. I have evaluated the structure of topoisomerase I C-terminal domain that consists of the active site tyrosine. My data indicate that this domain exists in a molten globule conformation with a fluctuating

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43

Lückerath, Thorsten [Verfasser]. "RAFT polymerization from DNA for DNA-polymer conjugates and higher-ordered DNA-polymer architectures / Thorsten Lückerath." Ulm : Universität Ulm, 2021. http://d-nb.info/1229993983/34.

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44

Li, Jian. "Mechanism of DNA Homologous Recombination through Studies of DNA Sliding Clamps, Clamp Loaders, and DNA Polymerases." Ohio University / OhioLINK, 2013. http://rave.ohiolink.edu/etdc/view?acc_num=ohiou1374835449.

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45

Manetto, Antonio. "I-Modified Nucleosides as DNA-Sugar Centered Radical Precursors II-DNA Excess Electron Transfer Studies III-A new Direct DNA Detection Method: DNA Photography." Diss., lmu, 2007. http://nbn-resolving.de/urn:nbn:de:bvb:19-83988.

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46

Tsusaka, Takeshi. "Methylation of DNA Ligase 1 by G9a/GLP Recruits UHRF1 to Replicating DNA and Regulates DNA Methylation." Kyoto University, 2018. http://hdl.handle.net/2433/232305.

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47

Amato, Nicholas J. "Impact of DNA Structure and Aeropyrum pernix Single-Strand DNA Binding Protein on Oxidative Damage to DNA." University of Toledo / OhioLINK, 2013. http://rave.ohiolink.edu/etdc/view?acc_num=toledo1372296254.

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48

Luo, Dan. "Novel crosslinking technologies to assess protein-DNA binding and DNA-DNA complexes for gene delivery and expression." The Ohio State University, 1997. http://rave.ohiolink.edu/etdc/view?acc_num=osu1114436532.

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49

Sherrer, Shanen Michelle. "Mutagenic and Kinetic Effects of Various DNA Lesions on DNA Polymerization Catalyzed by Y-Family DNA Polymerases." The Ohio State University, 2011. http://rave.ohiolink.edu/etdc/view?acc_num=osu1313178275.

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50

Luo, Dan. "Novel crosslinking technologies to assess protein-DNA binding and DNA-DNA complexes for gene delivery and expression /." Connect to resource, 1998. http://rave.ohiolink.edu/etdc/view?acc%5Fnum=osu1114436532.

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