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Artículos de revistas sobre el tema "Edna"

Autor: Grafiati

Publicado: 4 de junio de 2021

Última modificación: 25 de julio de 2025

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1

Nakamichi, Tomoki, Masahiro Ono, Masatoshi Hayashi, Takahiko Okamura, Toshihiro Wada, and Kenji Saitoh. "Environmental DNA Analysis in a River Detected a Possible Distribution of Fish Species Difficult to Capture." Fishes 8, no. 10 (2023): 496. http://dx.doi.org/10.3390/fishes8100496.

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Environmental DNA (eDNA) analysis is a biological survey method that has drawn much attention in recent years. However, the results of eDNA analysis and capture surveys often do not completely match, and the validity of the eDNA analysis needs to be verified. Verification of eDNA metabarcoding was conducted in a river in Fukushima Prefecture, Japan, in comparison with capture survey data. Most of the captured species were detected, and 13 uncaptured lineages (two genera and 11 species) were detected in the eDNAs. Some rare species detected in the eDNAs were also identified, including exotic ee

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Mori, Kensuke, Akio Imamura, Itsuki Hirayama, and Toshifumi Minamoto. "Detection of Echinococcus multilocularis in repurposed environmental DNA samples from river water." PeerJ 11 (June 14, 2023): e15431. http://dx.doi.org/10.7717/peerj.15431.

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Environmental DNA (eDNA) is an increasingly popular tool in biological and ecological studies. As a biproduct of its increasing use, large number of eDNA samples are being collected and stored, that potentially contain information of many non-target species. One potential use for these eDNA samples is a surveillance and early detection of pathogens and parasites that are otherwise difficult to detect. Echinococcus multilocularis is such a parasite with serious zoonotic concern, and whose range has been expanding. If eDNA samples from various studies can be repurposed in detecting the parasite,

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3

Nigliazzo, Stacy. "Edna." Annals of Internal Medicine 152, no. 2 (2010): 130. http://dx.doi.org/10.7326/0003-4819-152-2-201001190-00017.

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Zhang, Jing, Yu Zheng, and Jian Hua. "Effect of microplastics on the transport of extracellular DNA in an agricultural soil." E3S Web of Conferences 536 (2024): 03015. http://dx.doi.org/10.1051/e3sconf/202453603015.

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The transport of eDNA is one of the key environmental behaviors for its spreading and dispersal. Microplastics (MPs) are widely present in the soil environment and directly affect the environmental behavior of co-coexisting soil pollutants. However, the effect of MPs on eDNA transport and its mechanism remain unclear. In this study, we systematically investigated the effect of MPs types and functional groups on eDNA transport. The results showed that different kinds of MPs promoted eDNA transport, but there was no significant difference between these two MPs types. MPs with two different funct

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5

Paulson, C. "Edna Ashy." Insight - the Journal of the American Society of Ophthalmic Registered Nurses 26, no. 2 (2001): 51–52. http://dx.doi.org/10.1067/min.2001.115708.

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6

Hawkes, Mary Q. "Edna Mahan." Women & Criminal Justice 9, no. 3 (1998): 1–21. http://dx.doi.org/10.1300/j012v09n03_01.

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7

York, Ashley. "Electrified eDNA." Nature Reviews Microbiology 18, no. 10 (2020): 543. http://dx.doi.org/10.1038/s41579-020-0436-6.

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Lewenza, Shawn, Lori Johnson, Laetitia Charron-Mazenod, Mia Hong, and Heidi Mulcahy-O'Grady. "Extracellular DNA controls expression of Pseudomonas aeruginosa genes involved in nutrient utilization, metal homeostasis, acid pH tolerance and virulence." Journal of Medical Microbiology 69, no. 6 (2020): 895–905. http://dx.doi.org/10.1099/jmm.0.001184.

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Introduction. Pseudomonas aeruginosa grows in extracellular DNA (eDNA)-enriched biofilms and infection sites. eDNA is generally considered to be a structural biofilm polymer required for aggregation and biofilm maturation. In addition, eDNA can sequester divalent metal cations, acidify growth media and serve as a nutrient source. Aim. We wanted to determine the genome-wide influence on the transcriptome of planktonic P. aeruginosa PAO1 grown in the presence of eDNA. Methodology. RNA-seq analysis was performed to determine the genome-wide effects on gene expression of PAO1 grown with eDNA. Tran

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9

Ely, Taylor, Paul H. Barber, Lauren Man, and Zachary Gold. "Short-lived detection of an introduced vertebrate eDNA signal in a nearshore rocky reef environment." PLOS ONE 16, no. 6 (2021): e0245314. http://dx.doi.org/10.1371/journal.pone.0245314.

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Environmental DNA (eDNA) is increasingly used to measure biodiversity of marine ecosystems, yet key aspects of the temporal dynamics of eDNA remain unknown. Of particular interest is in situ persistence of eDNA signals in dynamic marine environments, as eDNA degradation rates have predominantly been quantified through mesocosm studies. To determine in situ eDNA residence times, we introduced an eDNA signal from a non-native fish into a protected bay of a Southern California rocky reef ecosystem, and then measured changes in both introduced and background eDNA signals across a fixed transect ov

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Adams, Clare, Luke Hoekstra, Morgan Muell, and Fredric Janzen. "A Brief Review of Non-Avian Reptile Environmental DNA (eDNA), with a Case Study of Painted Turtle (Chrysemys picta) eDNA Under Field Conditions." Diversity 11, no. 4 (2019): 50. http://dx.doi.org/10.3390/d11040050.

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Environmental DNA (eDNA) is an increasingly used non-invasive molecular tool for detecting species presence and monitoring populations. In this article, we review the current state of non-avian reptile eDNA work in aquatic systems, and present a field experiment on detecting the presence of painted turtle (Chrysemys picta) eDNA. Thus far, turtle and snake eDNA studies have shown mixed results in detecting the presence of these animals under field conditions. However, some instances of low detection rates and non-detection occur for these non-avian reptiles, especially for squamates. We explore

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11

Sansom, Brandon J., Dannise V. Ruiz-Ramos, Nathan L. Thompson, et al. "Detection and transport of environmental DNA from two federally endangered mussels." PLOS ONE 19, no. 10 (2024): e0304323. http://dx.doi.org/10.1371/journal.pone.0304323.

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Environmental DNA (eDNA) offers a novel approach to supplement traditional surveys and provide increased spatial and temporal information on species detection, and it can be especially beneficial for detecting at risk or threatened species with minimal impact on the target species. The transport of eDNA in lotic environments is an important component in providing more informed descriptions of where and when a species is present, but eDNA transport phenomena are not well understood. In this study, we used species-specific assays to detect eDNA from two federally endangered mussels in two geogra

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12

Pilliod, David S., Caren S. Goldberg, Robert S. Arkle, and Lisette P. Waits. "Estimating occupancy and abundance of stream amphibians using environmental DNA from filtered water samples." Canadian Journal of Fisheries and Aquatic Sciences 70, no. 8 (2013): 1123–30. http://dx.doi.org/10.1139/cjfas-2013-0047.

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Environmental DNA (eDNA) methods for detecting aquatic species are advancing rapidly, but with little evaluation of field protocols or precision of resulting estimates. We compared sampling results from traditional field methods with eDNA methods for two amphibians in 13 streams in central Idaho, USA. We also evaluated three water collection protocols and the influence of sampling location, time of day, and distance from animals on eDNA concentration in the water. We found no difference in detection or amount of eDNA among water collection protocols. eDNA methods had slightly higher detection

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13

Xin, Yi, Yu Guo, Meijing Sun, et al. "Optimal Conditions to Quantify the Relationship between eDNA Concentration and Biomass in Acanthopagrus latus." Water 14, no. 21 (2022): 3521. http://dx.doi.org/10.3390/w14213521.

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Environmental DNA (eDNA) analysis is a useful tool for monitoring the distributions of aquatic species. eDNA can produce quantitative estimates of fish abundance, but its accuracy depends on the species and system. Therefore, its performance must be evaluated and an investigation of how fish biomass affects eDNA dynamics must be conducted on a case-by-case basis. This study evaluates how the biomass of an ecologically and socioeconomically important fish, Acanthopagrus latus, relates to the eDNA concentration in aquariums. We conducted experiments using juvenile individuals and evaluated eDNA

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14

Park, Seongsik, Seokjin Yoon, and Kyunghoi Kim. "Numerical Study on Evaluation of Environmental DNA Approach for Estimating Fish Abundance and Distribution in Semi-Enclosed Bay." Journal of Marine Science and Engineering 12, no. 10 (2024): 1891. http://dx.doi.org/10.3390/jmse12101891.

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Despite efforts to use environmental DNA (eDNA), accurately quantifying fish populations remains a challenge. A recent eDNA approach provided reliable estimates of coastal fish population abundance, but it was not as effective for assessing spatial distribution due to a lack of eDNA samples relative to the study area. Therefore, we conducted a numerical case study to evaluate the ability of the eDNA approach to estimate fish (Jack mackerel) abundance and distribution based on the number of eDNA samples in a semi-enclosed bay (Jinhae Bay). Our study revealed that the eDNA approach can provide r

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15

Takahashi, Sayaka, Shingo Takada, Hiroki Yamanaka, Reiji Masuda, and Akihide Kasai. "Intraspecific genetic variability and diurnal activity affect environmental DNA detection in Japanese eel." PLOS ONE 16, no. 9 (2021): e0255576. http://dx.doi.org/10.1371/journal.pone.0255576.

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Environmental DNA (eDNA) analysis with species-specific primer/probe sets is promising as a tool to quantify fish abundance and distribution. Nevertheless, several factors could reduce the accuracy of this method. Here, we aimed to analyze whether intraspecific variability and diel activity rhythm affect eDNA detection in Japanese eels (Anguilla japonica). For this purpose, we performed tank experiments focusing on two points. First, we assessed the effects of base pair sequences with probe region polymorphism on eDNA detection. Next, we evaluated the influences of diel rhythm, activity, and i

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16

Thomas, Austen, and Caren Goldberg. "Evolution of a high-throughput environmental DNA sampling platform." ARPHA Conference Abstracts 4 (March 4, 2021): e65412. https://doi.org/10.3897/aca.4.e65412.

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With the rise of environmental DNA as a surveillance tool for aquatic species, a need has also arisen for professionally engineered research tools specifically designed for eDNA applications. We created the first portable, purpose-built eDNA sampling system in the form of a backpack smart-pump filtration apparatus and custom made eDNA filter packets for each sample. The eDNA-Sampler (previously ANDe) enables both point-location sampling and mobile sampling over a spatial distance, with the ability to standardize filtration parameters (e.g. flow rate, pressure, water volume, etc.). In this pres

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17

Kakuda, Aozora, Hideyuki Doi, Rio Souma, Mariko Nagano, Toshifumi Minamoto, and Izumi Katano. "Environmental DNA detection and quantification of invasive red-eared sliders, Trachemy scripta elegans, in ponds and the influence of water quality." PeerJ 7 (December 6, 2019): e8155. http://dx.doi.org/10.7717/peerj.8155.

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Environmental DNA (eDNA) is a powerful tool for monitoring the distribution of aquatic macro-organisms. However, environmental factors, including the water temperature and water quality, can affect the inhibition and/or degradation of eDNA, which complicates accurate estimations of eDNA concentrations and the detection of the presence/absence of species in natural habitats. Further very few eDNA studies have been conducted for reptiles, especially with respect to estimating their biomass and/or abundances. Here we examined the relationship between the visually-observed number of red-eared slid

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Klobucar, Stephen L., Torrey W. Rodgers, and Phaedra Budy. "At the forefront: evidence of the applicability of using environmental DNA to quantify the abundance of fish populations in natural lentic waters with additional sampling considerations." Canadian Journal of Fisheries and Aquatic Sciences 74, no. 12 (2017): 2030–34. http://dx.doi.org/10.1139/cjfas-2017-0114.

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Environmental DNA (eDNA) sampling has proven to be a valuable tool for detecting species in aquatic ecosystems. Within this rapidly evolving field, a promising application is the ability to obtain quantitative estimates of relative species abundance based on eDNA concentration rather than traditionally labor-intensive methods. We investigated the relationship between eDNA concentration and Arctic char (Salvelinus alpinus) abundance in five well-studied natural lakes; additionally, we examined the effects of different temporal (e.g., season) and spatial (e.g., depth) scales on eDNA concentratio

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19

Wang, Zhaoyue, Jiahui Xiao, Fengjie Hu, Qiao Yu, Taiping Zhang, and Shaoqi Zhou. "Adsorption of extracellular DNA to biochar derived from Chinese herbal medicine residues and impact on DNA degradation by DNase I." AIP Advances 12, no. 7 (2022): 075304. http://dx.doi.org/10.1063/5.0095208.

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The relationship between biochar physicochemical characteristics and the adsorption and the degradation of extracellular DNA (eDNA) was studied to assess controls on the fate and transport of eDNA in the environment. Biochar samples were generated by pyrolysis of Chinese herbal medicine residues of sweet wormwood ( Artemisia annua L.) at 500, 600, and 700 °C. Selected physicochemical properties of the biochar were characterized. Adsorption dynamics (adsorption capacity and kinetics) of eDNA to biochar were quantified using several adsorption kinetic and isotherm models. Furthermore, gel electr

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20

Havermans, Charlotte, Annkathrin Dischereit, Dmitrii Pantiukhin, Madlen Friedrich, and Ayla Murray. "ENVIRONMENTAL DNA IN AN OCEAN OF CHANGE: STATUS, CHALLENGES AND PROSPECTS." Arquivos de Ciências do Mar 55, Especial (2022): 298–337. http://dx.doi.org/10.32360/acmar.v55iespecial.78188.

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Environmental DNA (eDNA) studies have burgeoned over the last two decades and the application of eDNA has increased exponentially since 2010, albeit at a slower pace in the marine system. We provide a literature overview on marine metazoan eDNA studies and assess recent achievements in answering questions related to species distributions, biodiversity and biomass. We investigate which are the better studied taxonomic groups, geographic regions and the genetic markers used. We evaluate the use of eDNA for addressing ecological and environmental issues through food web, ecotoxicological, surveil

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Shogren, Arial J., Jennifer L. Tank, Elizabeth A. Andruszkiewicz, Brett Olds, Christopher Jerde, and Diogo Bolster. "Modelling the transport of environmental DNA through a porous substrate using continuous flow-through column experiments." Journal of The Royal Society Interface 13, no. 119 (2016): 20160290. http://dx.doi.org/10.1098/rsif.2016.0290.

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Detecting environmental DNA (eDNA) in water samples is a powerful tool in determining the presence of rare aquatic species. However, many open questions remain as to how biological and physical conditions in flowing waters influence eDNA. Motivated by what one might find in a stream/river benthos we conducted experiments in continuous flow columns packed with porous substrates to explore eDNA transport and ask whether substrate type and the presence of colonized biofilms plays an important role for eDNA retention. To interpret our data, and for modelling purposes, we began with the assumption

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Pinakhina, Daria V., and Elena M. Chekunova. "Environmental DNA: history of studies, current and perspective applications in fundamental and applied research." Ecological genetics 18, no. 4 (2020): 493–509. http://dx.doi.org/10.17816/ecogen25900.

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This review article is dedicated to a relatively young, actively developing approach to biodiversity assessment analysis of environmental DNA (or eDNA). Current views on the nature of eDNA, a brief overview of the history of this approach and methods of eDNA analysis are presented. Major research directions, utilizing eDNA techniques, and perspectives of their application to the study of biodiversity are described. Key issues in development of eDNA approach, its advantages and drawbacks are outlined.

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23

Shu, Lu, Arne Ludwig, and Zuogang Peng. "Standards for Methods Utilizing Environmental DNA for Detection of Fish Species." Genes 11, no. 3 (2020): 296. http://dx.doi.org/10.3390/genes11030296.

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Environmental DNA (eDNA) techniques are gaining attention as cost-effective, non-invasive strategies for acquiring information on fish and other aquatic organisms from water samples. Currently, eDNA approaches are used to detect specific fish species and determine fish community diversity. Various protocols used with eDNA methods for aquatic organism detection have been reported in different eDNA studies, but there are no general recommendations for fish detection. Herein, we reviewed 168 papers to supplement and highlight the key criteria for each step of eDNA technology in fish detection and

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Gu, Siyu, Peng Zhang, Shuai Luo, et al. "Microbial Community Colonization Process Unveiled through eDNA-PFU Technology in Mesocosm Ecosystems." Microorganisms 11, no. 10 (2023): 2498. http://dx.doi.org/10.3390/microorganisms11102498.

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Microbial communities are essential components of aquatic ecosystems and are widely employed for the detection, protection, and restoration of water ecosystems. The polyurethane foam unit (PFU) method, an effective and widely used environmental monitoring technique, has been improved with the eDNA-PFU method, offering efficiency, rapidity, and standardization advantages. This research aimed to explore the colonization process of microbial communities within PFUs using eDNA-PFU technology. To achieve this, we conducted ten-day monitoring and sequencing of microbial communities within PFUs in a

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Millard-Martin, Ben, Kate Sheridan, Evan Morien, et al. "Effect of environmental DNA sampling resolution in detecting nearshore fish biodiversity compared to capture surveys." PeerJ 12 (October 14, 2024): e17967. http://dx.doi.org/10.7717/peerj.17967.

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Sampling and sequencing marine environmental DNA (eDNA) provides a tool that can increase our ability to monitor biodiversity, but movement and mixing of eDNA after release from organisms before collection could affect our inference of species distributions. To assess how conditions at differing spatial scales influence the inferred species richness and compositional turnover, we conducted a paired eDNA metabarcoding and capture (beach seining) survey of fishes on the coast of British Columbia. We found more taxa were typically detected using eDNA compared to beach seining. eDNA identified mor

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Saito, Tatsuya, and Hideyuki Doi. "Degradation factors of environmental DNA evaluated by experiments and meta-analysis." ARPHA Conference Abstracts 4 (March 8, 2021): e65552. https://doi.org/10.3897/aca.4.e65552.

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Environmental DNA (eDNA) methods have been developed to detect organisms' distributions and abundance/biomass in various environments. eDNA degradation is critical for eDNA evaluation, but, the dynamics and mechanisms of eDNA degradation are largely unknown, especially when considering different eDNA sources, e.g., cell-derived and fragmental DNA. In this study, we conducted the degradation experiments (Saito and Doi 2020a) and a meta-analysis (Saito and Doi 2020b). Firstly, we experimentally evaluated the degradation rates of eDNA derived from multiple sources, including fragmental DNA (the D

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Sakata, Masayuki K., Megumi Sato, Marcello Otake Sato, et al. "Detection and persistence of environmental DNA (eDNA) of the different developmental stages of a vector mosquito, Culex pipiens pallens." PLOS ONE 17, no. 8 (2022): e0272653. http://dx.doi.org/10.1371/journal.pone.0272653.

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Preventing mosquito-borne infectious diseases requires that vector mosquitoes are monitored and controlled. Targeting immature mosquitoes (eggs, larvae, and pupae), which have less mobility than adults, is an effective management approach. However, conducting these surveys is often difficult due to the limitations of morphological classification and survey costs. The application of environmental DNA (eDNA) analysis can solve these issues because it allows easy estimation of species distribution and morphology-independent species identification. Although a few previous studies have reported mos

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Kim, Keonhee, Hyeonjin Cho, Jeong-Hui Kim, et al. "Environmental DNA in a Biofilm Can Be Used to Assess Diatom Ecological Health in Stream Water Ecology." Diversity 16, no. 1 (2023): 8. http://dx.doi.org/10.3390/d16010008.

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In urban and agricultural streams, assessing aquatic ecosystem health is critical due to widespread pollution. Traditional methods for evaluating attached diatoms crucial for ecosystem monitoring face limitations such as species misidentification and sample damage. This study was conducted in the Miho River within the Geum River system and highlights the effectiveness of environmental DNA (eDNA) techniques for more accurate and efficient genetic-based analysis than conventional microscopic analysis methods. When eDNA-based assessments were compared with traditional microscopic methods, this st

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Doi, Hideyuki. "Environmental DNA methods for the analysis of macroorganismal populations." ARPHA Conference Abstracts 4 (March 8, 2021): e65549. https://doi.org/10.3897/aca.4.e65549.

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Environmental DNA (eDNA) methods have been widely used to investigate the distribution and abundance/biomass of macroorganisms. eDNA methods analyze DNA collected directly from the environment, such as from water, soil, and air. The techniques have been applied to many taxa inhabiting various aquatic and terrestrial ecosystems. The recent development of eDNA methods has revolutionized the way we assess macroorganisms in natural environments.In this talk, I will present current developments of eDNA methodology, especially with regard to population analysis using various DNA measurement methods.

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Hervé, Alix, Isabelle Domaizon, Jean-Marc Baudoin, et al. "Spatio-temporal variability of eDNA signal and its implication for fish monitoring in lakes." PLOS ONE 17, no. 8 (2022): e0272660. http://dx.doi.org/10.1371/journal.pone.0272660.

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Environmental DNA (eDNA) metabarcoding is revolutionizing the monitoring of aquatic biodiversity. The use of eDNA has the potential to enable non-invasive, cost-effective, time-efficient and high-sensitivity monitoring of fish assemblages. Although the capacity of eDNA metabarcoding to describe fish assemblages is recognised, research efforts are still needed to better assess the spatial and temporal variability of the eDNA signal and to ultimately design an optimal sampling strategy for eDNA monitoring. In this context, we sampled three different lakes (a dam reservoir, a shallow eutrophic la

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Das, Theerthankar, Prashant K. Sharma, Henk J. Busscher, Henny C. van der Mei, and Bastiaan P. Krom. "Role of Extracellular DNA in Initial Bacterial Adhesion and Surface Aggregation." Applied and Environmental Microbiology 76, no. 10 (2010): 3405–8. http://dx.doi.org/10.1128/aem.03119-09.

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ABSTRACT Extracellular DNA (eDNA) is an important component of the biofilm matrix. We show that removal of eDNA from Gram-positive bacteria reduces initial adhesion to and aggregation of bacteria on surfaces. Thermodynamic analyses indicated that eDNA introduces favorable acid-base interactions, explaining the effect of eDNA on aggregation and adhesion to the surface.

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Li, Wen-Pan, Zi-Fang Liu, Tong Guo, He Chen, and Xin Xie. "Using Optimal Environmental DNA Method to Improve the Fish Diversity Survey—From Laboratory to Aquatic Life Reserve." Water 13, no. 11 (2021): 1468. http://dx.doi.org/10.3390/w13111468.

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Conserving aquatic ecosystems requires efficient tools to accurately assess the biodiversity of aquatic species. However, existing knowledge is insufficient in terms of the reliability and the comparability of methods measuring fish diversity. Environmental DNA (eDNA), as a promising method, was used to detect fish taxa in this study. We optimized the eDNA method in the laboratory, and applied the optimal eDNA method to survey fish diversity in a natural aquatic life reserve. We simulated necessary steps of the eDNA method in the lab to increase the confidence of the field survey. Specifically

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Jones, Rhys Aled, Chelsea N. Davis, Dewi Llyr Jones, et al. "Temporal dynamics of trematode intermediate snail host environmental DNA in small water body habitats." Parasitology 148, no. 12 (2021): 1490–96. http://dx.doi.org/10.1017/s0031182021001104.

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AbstractEnvironmental DNA (eDNA) surveying has potential to become a powerful tool for sustainable parasite control. As trematode parasites require an intermediate snail host that is often aquatic or amphibious to fulfil their lifecycle, water-based eDNA analyses can be used to screen habitats for the presence of snail hosts and identify trematode infection risk areas. The aim of this study was to identify climatic and environmental factors associated with the detection of Galba truncatula eDNA. Fourteen potential G. truncatula habitats on two farms were surveyed over a 9-month period, with eD

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Iwai, Noriko, Kiyomi Yasumiba, and Teruhiko Takahara. "Efficacy of environmental DNA to detect and quantify stream tadpoles of Odorrana splendida." Royal Society Open Science 6, no. 1 (2019): 181798. http://dx.doi.org/10.1098/rsos.181798.

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Environmental DNA (eDNA) can be used to detect and estimate the density of rare or secretive species, especially in aquatic systems. However, the efficacy of eDNA method has not been validated in lotic systems. We examined the efficacy of the eDNA method to detect and estimate abundance and biomass of a stream-dwelling frog species, Odorrana splendida . We conducted eight field surveys over 2 years and obtained 53 water samples from 10 streams with known distribution of O. splendida tadpoles. The eDNA method accurately detected the presence of O. splendida in 79.2% of survey samples. The amoun

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Uchida, Noriko, Kengo Kubota, Shunsuke Aita, and So Kazama. "Aquatic insect community structure revealed by eDNA metabarcoding derives indices for environmental assessment." PeerJ 8 (June 11, 2020): e9176. http://dx.doi.org/10.7717/peerj.9176.

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Environmental DNA (eDNA) analysis provides an efficient and objective approach for monitoring and assessing ecological status; however, studies on the eDNA of aquatic insects, such as Ephemeroptera, Plecoptera, and Trichoptera (EPT), are limited despite its potential as a useful indicator of river health. Here, we investigated the community structures of aquatic insects using eDNA and evaluated the applicability of eDNA data for calculating assessment indices. Field surveys were conducted to sample river water for eDNA at six locations from upstream to downstream of two rivers in Japan in July

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Shao, Yun, Shuping Wang, Pengyuan Wang, et al. "Environmental DNA in different media reveals distribution characteristics and assembly mechanisms of fish assemblages in a complex river–lake system." Web Ecology 24, no. 2 (2024): 59–70. http://dx.doi.org/10.5194/we-24-59-2024.

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Abstract. Capture-based methods are commonly used for biomonitoring fish assemblages in freshwater. The recent advancement in environmental DNA (eDNA) metabarcoding provides a sensitive, cost-effective, and non-intrusive alternative to traditional methods. Nevertheless, the effectiveness of this approach in river–lake systems has yet to be assessed, and there is ongoing debate regarding the selection of sampling media. In this study, we investigated fish assemblages based on traditional approaches and the eDNA metabarcoding method by analyzing water and sediment from 30 locations along the Bai

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DeHart, Hayley M., Mark T. Gasser, Jarret Dixon, and Peter Thielen. "An aquatic environmental DNA filtration system to maximize recovery potential and promote filtration approach standardization." PeerJ 11 (July 12, 2023): e15360. http://dx.doi.org/10.7717/peerj.15360.

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Background Aquatic environmental DNA (eDNA) has emerged as a promising approach to identify organisms in freshwater and marine environments. While the recovery of eDNA from water most commonly involves capture of biological debris on a filter matrix, practitioners are yet to converge on standardized approaches for filtration, particularly in the field. This lack of standardization has resulted in inconsistent handling of samples following collection, limiting interpretation of results across studies and burdening groups with inconvenient storage and transport logistics that may compromise eDNA

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Wilton, Mike, Tyler W. R. Halverson, Laetitia Charron-Mazenod, Michael D. Parkins, and Shawn Lewenza. "Secreted Phosphatase and Deoxyribonuclease Are Required by Pseudomonas aeruginosa To Defend against Neutrophil Extracellular Traps." Infection and Immunity 86, no. 9 (2018). http://dx.doi.org/10.1128/iai.00403-18.

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ABSTRACT Neutrophil extracellular traps (NETs) are produced by neutrophils as an innate immune defense mechanism to trap and kill microbial pathogens. NETs are comprised of ejected chromatin that forms a lattice structure enmeshed with numerous antimicrobial proteins. In addition to forming the structural backbone of NETs, extracellular DNA (eDNA) has membrane-disrupting antimicrobial activity that contributes to NET killing. Many pathogens produce secreted extracellular DNases to evade the antimicrobial activity of NETs. Pseudomonas aeruginosa encodes an operon of two secreted enzymes, a pred

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Sato, Masaaki, Nariaki Inoue, Ryogen Nambu, Naoki Furuichi, Tomohito Imaizumi, and Masayuki Ushio. "Quantitative assessment of multiple fish species around artificial reefs combining environmental DNA metabarcoding and acoustic survey." Scientific Reports 11, no. 1 (2021). http://dx.doi.org/10.1038/s41598-021-98926-5.

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AbstractSince the early 1970s, many artificial reefs (ARs) have been deployed in Japanese coastal waters to create fisheries grounds. Recently, researchers began to use environmental DNA (eDNA) methods for biodiversity monitoring of aquatic species. A metabarcoding approach using internal standard DNAs [i.e., quantitative MiSeq sequencing (qMiSeq)] makes it possible to monitor eDNA concentrations of multiple species simultaneously. This method can improve the efficiency of monitoring AR effects on fishes. Our study investigated distributions of marine fishes at ARs and surrounding stations in

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Rose, Sasha J., and Luiz E. Bermudez. "Identification of Bicarbonate as a Trigger and Genes Involved with Extracellular DNA Export in Mycobacterial Biofilms." mBio 7, no. 6 (2016). http://dx.doi.org/10.1128/mbio.01597-16.

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ABSTRACTExtracellular DNA (eDNA) is an integral biofilm matrix component of numerous pathogens, including nontuberculous mycobacteria (NTM). Cell lysis is the source of eDNA in certain bacteria, but the source of eDNA remains unidentified for NTM, as well as for other eDNA-containing bacterial species. In this study, conditions affecting eDNA export were examined, and genes involved with the eDNA export mechanism were identified. After a method for monitoring eDNA in real time in undisturbed biofilms was established, different conditions affecting eDNA were investigated. Bicarbonate positively

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Cherny, Kathryn E., and Karin Sauer. "Pseudomonas aeruginosa Requires the DNA-Specific Endonuclease EndA To Degrade Extracellular Genomic DNA To Disperse from the Biofilm." Journal of Bacteriology 201, no. 18 (2019). http://dx.doi.org/10.1128/jb.00059-19.

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ABSTRACT The dispersion of biofilms is an active process resulting in the release of planktonic cells from the biofilm structure. While much is known about the process of dispersion cue perception and the subsequent modulation of the c-di-GMP pool, little is known about subsequent events resulting in the release of cells from the biofilm. Given that dispersion coincides with void formation and an overall erosion of the biofilm structure, we asked whether dispersion involves degradation of the biofilm matrix. Here, we focused on extracellular genomic DNA (eDNA) due to its almost universal prese

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Liu, Yan, Mengyi Zhang, Liangming Wang, et al. "Experimental assessment of Acanthopagrus schlegelii biomass based on environmental DNA technology." Scientific Reports 14, no. 1 (2024). https://doi.org/10.1038/s41598-024-83590-2.

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AbstractThe Environmental DNA (eDNA) technology has attracted significant attention due to its convenience and high sensitivity. However, the variations of eDNA across diverse environments and biological species remain complex. Therefore, a detailed exploration of the release patterns of eDNA for specific species under different environments is crucial for the scientific utilization of eDNA detection techniques. This study conducted an experiment involving the aquaculture of Acanthopagrus schlegelii to explore the release and degradation mechanisms of eDNA. It also analyzed the influence of sa

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Brandão‐Dias, Pedro F. P., Elise Snyder, Jennifer L. Tank, et al. "Comparing the Fate of eDNA by Particle Sizes and Molecule Lengths in Recirculating Streams." Environmental DNA 7, no. 2 (2025). https://doi.org/10.1002/edn3.70066.

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ABSTRACTThe detection of environmental DNA (eDNA) has revolutionized aquatic species monitoring, yet interpreting eDNA data remains challenging due to gaps in our understanding of eDNA ecology (i.e., origin, state, transport, and fate) and variability in how eDNA methods are applied across the literature. A crucial aspect of the complexity of eDNA ecology is that eDNA is a heterogeneous mix of components that vary in size and other properties, thereby influencing interactions with the environment in diverse ways. In this study, we explore the interplay between three eDNA particle sizes (the ph

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Hirohara, Takaya, Kenji Tsuri, Koichi Miyagawa, Robert T. R. Paine, and Hiroki Yamanaka. "The Application of PMA (Propidium Monoazide) to Different Target Sequence Lengths of Zebrafish eDNA: A New Approach Aimed Toward Improving Environmental DNA Ecology and Biological Surveillance." Frontiers in Ecology and Evolution 9 (June 4, 2021). http://dx.doi.org/10.3389/fevo.2021.632973.

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Environmental DNA (eDNA) analysis has enabled more sensitive and efficient biological monitoring than traditional methods. However, since the target species is not directly observed, interpretation of results cannot preclude process Type I errors. Specifically, there may be a spatial or temporal gap between the target eDNA and the eDNA source in the sampled area. Moreover, eDNA surveillance lacks the ability to distinguish whether eDNA originated from a living or non-living source. This kind of Type I error is difficult to control for, in part, because the relationship between the state of eDN

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Bonk, Eric A., Robert H. Hanner, Adrienne J. Bartlett, and Gerald R. Tetreault. "Environmental DNA Metabarcoding Detects Predators at Higher Rates Than Electrofishing." Environmental DNA 6, no. 5 (2024). http://dx.doi.org/10.1002/edn3.70019.

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ABSTRACTThere are numerous downsides and risks associated with electrofishing; hence, environmental DNA (eDNA) metabarcoding is becoming increasingly common in aquatic ecological studies. Generally, researchers agree that eDNA metabarcoding is more sensitive than electrofishing, and that eDNA metabarcoding is better at detecting rare species. As predatory species tend to be rarer than prey species, eDNA metabarcoding should hypothetically detect more predator species than electrofishing. Instead of supporting the notion that eDNA must replace electrofishing, or that eDNA and electrofishing mus

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Allan, Elizabeth Andruszkiewicz, Michelle H. DiBenedetto, Andone C. Lavery, Annette F. Govindarajan, and Weifeng G. Zhang. "Modeling characterization of the vertical and temporal variability of environmental DNA in the mesopelagic ocean." Scientific Reports 11, no. 1 (2021). http://dx.doi.org/10.1038/s41598-021-00288-5.

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AbstractIncreasingly, researchers are using innovative methods to census marine life, including identification of environmental DNA (eDNA) left behind by organisms in the water column. However, little is understood about how eDNA is distributed in the ocean, given that organisms are mobile and that physical and biological processes can transport eDNA after release from a host. Particularly in the vast mesopelagic ocean where many species vertically migrate hundreds of meters diurnally, it is important to link the location at which eDNA was shed by a host organism to the location at which eDNA

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West, Katrina M., Tyson R. Jones, Lara Denis‐Roy, et al. "Continual Day–Night eDNA Detectability Amidst Diel Reef Species Fluctuations on Diver Transects." Environmental DNA 6, no. 5 (2024). http://dx.doi.org/10.1002/edn3.70018.

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ABSTRACTRecent research into the spatiotemporal dynamics of eDNA in marine environments indicates that eDNA signals are highly localized and may dissipate beyond detection levels within a few hours of production. This affects whether single‐timepoint eDNA sampling, which generally occurs during daylight hours, or cyclic (day/night) interval eDNA sampling is necessary to detect both diurnal and nocturnal marine species. Our study investigated short‐term variability in eDNA derived from fishes and macroinvertebrates across three temperate reef sites in eastern Tasmania, Australia. Simultaneous e

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Sauseng, Gabriele, and Tamara Schenekar. "Water Interaction Type Affects Environmental DNA Shedding Rates of Terrestrial Mammal eDNA Into Surface Water Bodies." Environmental DNA 7, no. 3 (2025). https://doi.org/10.1002/edn3.70120.

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ABSTRACTThe analysis of environmental DNA (eDNA) has become a non‐invasive, cost‐efficient, and universal biomonitoring tool, widely applied across the globe. Most eDNA research focuses on aquatic organisms in freshwater and marine environments. eDNA shedding rates are key to interpreting eDNA‐based results, such as for abundance estimations or detection probabilities. Shedding rates have been estimated for several species and life stages; however, virtually all of them are aquatic. As eDNA‐based biomonitoring expands to terrestrial systems, waterborne eDNA from freshwater is increasingly used

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Pathan, Shamina Imran, Paola Arfaioli, Eren Taskin, Maria Teresa Ceccherini, Edoardo Puglisi, and Giacomo Pietramellara. "The extracellular DNA can baffle the assessment of soil bacterial community, but the effect varies with microscale spatial distribution." FEMS Microbiology Letters 368, no. 12 (2021). http://dx.doi.org/10.1093/femsle/fnab074.

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ABSTRACT Environmental DNA is made-up of intracellular (iDNA) and extracellular (eDNA) pools. In soils, eDNA can be present up to 40% and could distort the assessment of living microorganisms. Distribution of microbial community is inconsistent among different size-aggregates, and the persistence and turnover of eDNA are thus uneven. Uneven persistence and distribution of eDNA could lead to heterogeneity in community analysis biases that arise due to eDNA sequences at micro-scale distribution. Here, we investigated the diversity and structure of eDNA and iDNA bacterial communities in bulk soil

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Parsley, Meghan B., Erica J. Crespi, Tracy A. G. Rittenhouse, Jesse L. Brunner, and Caren S. Goldberg. "Environmental DNA concentrations vary greatly across productive and degradative conditions, with implications for the precision of population estimates." Scientific Reports 14, no. 1 (2024). http://dx.doi.org/10.1038/s41598-024-66732-4.

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AbstractPopulation size is an important metric to inform the conservation and management of species. For aquatic species, environmental DNA (eDNA) concentration has been suggested for non-invasively estimating population size. However, many biotic and abiotic factors simultaneously influence the production and degradation of eDNA which can alter the relationship between population size and eDNA concentration. We investigated the influence of temperature, salinity, and ranavirus infection on eDNA concentrations using tadpole mesocosms. Using linear regression models, we tested the influence of

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