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Tesis sobre el tema "Enzyme"

Autor: Grafiati

Publicado: 4 de junio de 2021

Última modificación: 25 de julio de 2025

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1

Ekici, Ozlem Dogan. "Design, synthesis, and evaluation of novel irreversible inhibitors for caspases." Diss., Georgia Institute of Technology, 2003. http://hdl.handle.net/1853/5333.

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2

Finnigan, William John Andrew. "The exploitation of thermophiles and their enzymes for the construction of multistep enzyme reactions from characterised enzyme parts." Thesis, University of Exeter, 2016. http://hdl.handle.net/10871/27323.

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Biocatalysis is a field rapidly expanding to meet a demand for green and sustainable chemical processes. As the use of enzymes for synthetic chemistry becomes more common, the construction of multistep enzyme reactions is likely to become more prominent providing excellent cost and productivity benefits. However, the design and optimisation of multistep reactions can be challenging. An enzyme toolbox of well-characterised enzyme parts is critical for the design of novel multistep reactions. Furthermore, while whole-cell biocatalysis offers an excellent platform for multistep reactions, we are

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3

Müller, Roger. "Artificial enzymes: from catalytic antibodies toward de novo enzyme design /." Zürich : ETH, 2008. http://e-collection.ethbib.ethz.ch/show?type=diss&nr=17897.

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4

Reichstädter, Marek. "Imobilizace vybraných glykanohydroláz." Master's thesis, Vysoké učení technické v Brně. Fakulta chemická, 2015. http://www.nusl.cz/ntk/nusl-217152.

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The theoretical part of this thesis deals with cellulolytic enzymes, their microbial producers, the possibilities of using such enzymes in the industry and how can be enzymes - not only cellulolytic - immobilized. Experimental part examines the preparations created by immobilizing various amounts of the commercially used cellulolytic complex Cellulast 1.5L onto various synthetic carriers made of polyethylene terephthalate - commercially used Sorsilen, PET carrier and glutaraldehyde-treated PET carrier. Enzyme activity of these preparations was determined by Somogyi - Nelson method by spectroph

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5

Ekici, Özlem Doğan. "Design, synthesis, and evaluation of novel irreversible inhibitors for caspases." Available online, Georgia Institute of Technology, 2004:, 2003. http://etd.gatech.edu/theses/available/etd-04062004-164633/unrestricted/ekici%5Fozlem%5Fd%5F200312%5Fphd.pdf.

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6

Obrecht, Lorenz. "Artificial metalloenzymes in catalysis." Thesis, University of St Andrews, 2015. http://hdl.handle.net/10023/7248.

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This thesis describes the synthesis, characterisation and application of artificial metalloenzymes as catalysts. The focus was on two mutants of SCP-2L (SCP-2L A100C and SCP-2L V83C) both of which possess a hydrophobic tunnel in which apolar substrates can accumulate. The crystal structure of SCP-2L A100C was determined and discussed with a special emphasis on its hydrophobic tunnel. The SCP-2L mutants were covalently modified at their unique cysteine with two different N-ligands (phenanthroline or dipicolylamine based) or three different phosphine ligands (all based on triphenylphosphine) in

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7

Bodhe, A. M. "Enzyme inhibitors." Thesis(Ph.D.), CSIR-National Chemical Laboratory, Pune, 1988. http://dspace.ncl.res.in:8080/xmlui/handle/20.500.12252/3302.

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8

Qian, Yuhui. "Study of Basic Wood Decay Mechanisms and Their Biotechnological Applications." Fogler Library, University of Maine, 2008. http://www.library.umaine.edu/theses/pdf/QianY2008.pdf.

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9

Zhao, Xueyan. "Nanoscale biocatalysts for bioelectrochemical applications." Akron, OH : University of Akron, 2006. http://rave.ohiolink.edu/etdc/view?acc%5Fnum=akron1164149161.

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Thesis (M.S.)--University of Akron, Dept. of Chemical Engineering, 2006.<br>"December, 2006." Title from electronic thesis title page (viewed 06/27/2007) Advisor, Ping Wang; Committee members, Lu-Kwang Ju, Steven S. C. Chuang; Department Chair, Lu-Kwang Ju; Dean of the College, George K. Haritos; Dean of the Graduate School, George R. Newkome. Includes bibliographical references.

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10

Moore, Robert Goodwin Douglas C. "Towards the understanding of complex biochemical systems the significance of global protein structure and thorough parametric analysis /." Auburn, Ala, 2009. http://hdl.handle.net/10415/1766.

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11

Astier, Yann. "Enzyme kinetics and electrochemical polymer transistor detection of enzyme reactions." Thesis, University of Southampton, 2001. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.273800.

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12

Ewing, Erin. "In vacuo glycation of enzymes: A novel approach for increasing enzyme stability." Thesis, University of Ottawa (Canada), 2006. http://hdl.handle.net/10393/27129.

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A novel approach for the thermostabilization of proteins was investigated. It is well established that proteins that are naturally highly glycosylated show an increased resistance to inactivation at high temperatures. However, non-enzymatic attachment of carbohydrate to proteins, otherwise known as protein glycation, under aqueous conditions is very difficult and has not been used as a general approach for increasing the thermostability of proteins. In the present study, advantage was taken of the in vacuo protein glycation procedure recently developed by Kaplan and his co-workers by which pro

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13

Mao, Wei. "Etude biochimique et sélection d'inhibiteurs spécifiques d'une cible thérapeutique leishmanienne : la GDP-Mannose-Pyrophosphorylase." Thesis, Université Paris-Saclay (ComUE), 2016. http://www.theses.fr/2016SACLS481.

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Les leishmanioses sont des maladies tropicales négligées provoquées par un protozoaire parasite du genre Leishmania, et transmises par un insecte vecteur, le phlébotome. Les leishmanioses menacent 310 millions de personnes dans 98 pays à travers le monde. Les traitements antileishmaniens actuels sont limités et présentent des problèmes majeurs de toxicité et d'émergence de chimiorésistance. Dans ce contexte, il est nécessaire de développer de nouveaux agents antileishmaniens spécifiquement dirigés contre une cible thérapeutique chez le parasite. La GDP-Mannose Pyrophosphorylase (GDP-MP) est un

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14

Robertson, Graeme. "Enzyme pesticide biosensors." Thesis, University of Strathclyde, 2001. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.366815.

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15

Birkin, Peter Robert. "Microelectrochemical enzyme transistors." Thesis, University of Southampton, 1994. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.240628.

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16

Wilson, Robert. "Enzyme amplified immunoassays." Thesis, Cranfield University, 1989. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.237805.

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17

Al-Lolage, Firas Ahmed Thanon. "Amperometric enzyme electrodes." Thesis, University of Southampton, 2018. https://eprints.soton.ac.uk/419053/.

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This thesis studies the conditions required to achieve direct electron transfer and the experimental tests needed to unequivocally demonstrate that it occurs. Many publications claim to observe direct electron transfer to redox enzymes (for example in the case of glucose oxidase) but the evidence presented is often incomplete and unconvincing. The first part of this thesis argues that the vast majority, if not all, of these claims of DET for GOx are incorrect. It presents results for glucose oxidase (GOx) adsorbed on multi‐walled carbon nanotubes (MWCNTs), a typical nanostructured GOx electrod

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18

Bolon, Daniel N. Goddard William A. "Computational enzyme design /." Diss., Pasadena, Calif. : California Institute of Technology, 2002. http://resolver.caltech.edu/CaltechETD:etd-01252002-100801.

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19

SILVA, JOSE A. A. da. "Aspectos de atividade biologica da giroxina (enzima trombina simile) isolada do veneno da cascavel brasileira, Crotalus durissus terrificus." reponame:Repositório Institucional do IPEN, 2004. http://repositorio.ipen.br:8080/xmlui/handle/123456789/11191.

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Made available in DSpace on 2014-10-09T12:49:21Z (GMT). No. of bitstreams: 0<br>Made available in DSpace on 2014-10-09T14:02:30Z (GMT). No. of bitstreams: 1 09994.pdf: 3905779 bytes, checksum: 7cdc8d8ae9585729a6a818ed202c59c8 (MD5)<br>Dissertacao (Mestrado)<br>IPEN/D<br>Instituto de Pesquisas Energeticas e Nucleares - IPEN/CNEN-SP

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20

Epstein, Todd Matthew. "Structural and kinetic studies of two enzymes catalyzing phospholipase A2 activity." Access to citation, abstract and download form provided by ProQuest Information and Learning Company; downloadable PDF file 2.39 Mb., 186 p, 2006. http://gateway.proquest.com/openurl?url_ver=Z39.88-2004&res_dat=xri:pqdiss&rft_val_fmt=info:ofi/fmt:kev:mtx:dissertation&rft_dat=xri:pqdiss:3200538.

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21

Snider, Catherine E. "Synthesis and biochemical evaluation of irreversible inhibitors of aromatase /." The Ohio State University, 1986. http://rave.ohiolink.edu/etdc/view?acc_num=osu1487266362338344.

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22

Haileselassie, Seble Sereke Berhan. "Production of enzyme-modified cheese and bioactive peptides by Lactobacillus and commercial enzymes." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1999. http://www.collectionscanada.ca/obj/s4/f2/dsk2/ftp03/MQ50782.pdf.

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23

Dong, Liang-Chang. "Thermally reversible hydrogels for controlled drug delivery and enzyme immobilization /." Thesis, Connect to this title online; UW restricted, 1990. http://hdl.handle.net/1773/8009.

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24

O'Neil, Crystal L. "Enzyme Exploitation: Manipulating Enzyme Function for Therapy, Synthesis and Natural Product Modification." The Ohio State University, 2011. http://rave.ohiolink.edu/etdc/view?acc_num=osu1293722936.

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25

Friemann, Rosmarie. "Structure-function studies of iron-sulfur enzyme systems /." Uppsala : Dept. of Molecular Biology, Swedish Univ. of Agricultural Sciences, 2005. http://epsilon.slu.se/a504.pdf.

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26

Williams, Simon-Peter. "Studies of enzyme kinetics and aspects of enzyme structure in vivo using NMR and molecular genetics." Thesis, University of Oxford, 1992. http://ora.ox.ac.uk/objects/uuid:d8baa574-a5d4-45a2-95a2-c141fbf8d277.

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A quantitative understanding of metabolic control depends on a knowledge of the enzymes involved. The extrapolation of studies in vitro to the intact cell is controversial because the intracellular environment is relatively poorly characterised, particularly with respect to the interactions between weakly-associated enzymes. There is a clear need to study enzymes directly in the cell, yet there are few suitable techniques. Metabolites have been very successfully studied in cells by the non-invasive technique of nuclear magnetic resonance (NMR). NMR studies of enzymes in the cell have, however,

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27

Lee, Charles Kai-Wu. "Eurythermalism of a deep-sea symbiosis system from an enzymological aspect." The University of Waikato, 2007. http://hdl.handle.net/10289/2588.

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The recently proposed and experimentally validated Equilibrium Model provides the most detailed description of temperature's effect on enzyme catalytic activity to date. By introducing an equilibrium between Eact, the active form of enzyme, and Einact, a reversibly inactivated form of enzyme, the Equilibrium Model explains apparent enzyme activity loss at high temperatures that cannot be accounted for by irreversible thermal denaturation. The Equilibrium Model describes enzyme behavior in the presence of substrates and under assay conditions; thus its associated parameters, deltaHeq and Teq,

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28

Gavva, Sandhya Reddy. "Alternate Substrates and Isotope Effects as a Probe of the Malic Enzyme Reaction." Thesis, University of North Texas, 1988. https://digital.library.unt.edu/ark:/67531/metadc330840/.

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Dissociation constants for alternate dirmcleotide substrates and competitive inhibitors suggest that the dinucleotide binding site of the Ascaris suum NAD-malic enzyme is hydrophobic in the vicinity of the nicotinamide ring. Changes in the divalent metal ion activator from Mg^2+ to Mn^2+ or Cd^2+ results in a decrease in the dinucleotide affinity and an increase in the affinity for malate. Primary deuterium and 13-C isotope effects obtained with the different metal ions suggest either a change in the transition state structure for the hydride transfer or decarboxylation steps or both. Deuteriu

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29

Peters, Thilo. "Extrazelluläre Enzyme aus Basidiomyceten." [S.l. : s.n.], 2004. http://deposit.ddb.de/cgi-bin/dokserv?idn=97236191X.

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30

YAMANE, Tsuneo. "Enzyme Engineering for Lipids." 名古屋大学農学国際教育協力研究センター, 2004. http://hdl.handle.net/2237/8930.

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31

Williams, Richard James. "Enzyme assisted self-assembly." Thesis, University of Manchester, 2008. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.496231.

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Self-assembling peptide systems provide a pathway for the formation of complex molecular assemblies from relatively simple designed molecules. Stimuli which have been used to trigger the self-assembly (SA) process in aqueous conditions include temperature, pH, ionic strength, and solvent exchange. Additionally, enzymes may be used to selectively control the self-assembly process; enzymes are uniquely chemo-, regio-, and enantioselective, and work naturally under mild conditions without disrupting biological Interactions.

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32

Beh, Seng Kee. "Membranes for enzyme electrodes." Thesis, Cardiff University, 1990. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.305091.

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33

Zaman, Flora. "Kinetics of enzyme models." Thesis, University of Kent, 1998. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.263701.

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34

Parratt, Julian Simon. "Enzyme catalysed nitrile hydrolysis." Thesis, University of Exeter, 1993. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.357898.

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35

Dennison, Manus. "Gas-phase enzyme biosensors." Thesis, Cranfield University, 1995. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.309584.

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36

Hall, Geoffrey F. "Organic phase enzyme electrodes." Thesis, Cranfield University, 1990. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.278720.

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37

Saini, S. "Organic phase enzyme electrodes." Thesis, Cranfield University, 1993. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.332925.

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38

Kalia, Yogeshvar Nath. "Development of enzyme electrodes." Thesis, Imperial College London, 1991. http://hdl.handle.net/10044/1/46856.

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39

Coote, Alexander Stuart. "Polyurethanes for enzyme immobilisation." Thesis, Imperial College London, 1989. http://hdl.handle.net/10044/1/47386.

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40

Sutar, I. I. "Studies on proteolytic enzyme." Thesis(Ph.D.), CSIR-National Chemical Laboratory, Pune, 1987. http://dspace.ncl.res.in:8080/xmlui/handle/20.500.12252/3285.

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41

Oudshoorn, Matthew Leslie. "Tests of predictions made by the Equilibrium Model for the effect of temperature on enzyme activity." The University of Waikato, 2008. http://hdl.handle.net/10289/2418.

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The Classical Model describing the effects of temperature on enzyme activity consists of two processes: the catalytic reaction defined by ΔG cat and irreversible inactivation defined by ΔG inact, this model however, does not account for the observed temperature- dependant behaviour of enzymes. The recent development of the Equilibrium Model is governed not only by ΔG cat and ΔG inact but also by two new intrinsic parameters ΔHeq and Teq, which describe the enthalpy and the temperature of the midpoint, respectively, of a active and reversibly inactive enzyme transition. Teq is central to the ph

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42

Silveira, Alvito J. "Synthesis of 6-guanidinobenzoxazinones as potential inhibitors of trypsin-like enzymes." Thesis, Georgia Institute of Technology, 1989. http://hdl.handle.net/1853/26914.

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43

Garrett, Mark Denis. "A study on selectivity in microbial biotransformations of substituted arenes." Thesis, Queen's University Belfast, 1999. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.287620.

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44

Sha, Zhou. "A Chemical Approach to Detect and Characterize The Activities of Mitochondrial ATP-dependent Protease Lon and ClpXP." Case Western Reserve University School of Graduate Studies / OhioLINK, 2020. http://rave.ohiolink.edu/etdc/view?acc_num=case1595239191845497.

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45

Fisher, Oriana. "Subcloning, enzymatic characterization, and in silico docking of transglutaminase 2." Waltham, Mass. : Brandeis University, 2009. http://dcoll.brandeis.edu/handle/10192/23253.

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46

Vögeli, Bastian [Verfasser], and Tobias [Akademischer Betreuer] Erb. "Control of reactive intermediates in enzymes and enzyme complexes / Bastian Vögeli ; Betreuer: Tobias Erb." Marburg : Philipps-Universität Marburg, 2019. http://d-nb.info/1185068716/34.

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47

Maheshwari, Sweta. "Caractérisation biochimique et cellulaire des enzymes clés du métabolisme des phospholipides chez Plasmodium falciparum." Thesis, Montpellier 2, 2012. http://www.theses.fr/2012MON20004.

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Le développement du parasite Plasmodium falciparum, responsable du paludisme, nécessite la synthèse de phospholipides et plus particulièrement de phosphatidylcholine (PC) et phosphaditylethanolamine (PE) qui représentent environ 85% de la totalité des phospholidipes du parasite. Leur synthèse s'effectue principalement par les voies métaboliques de novo, voies de Kennedy, en trois étapes enzymatiques. Les enzymes CTP: phosphoethanolamine cytidylyltransferase (ECT) et CTP: phosphocholine cytidylyltransferase (CCT) catalysent les étapes limitantes des deux voies de biosynthèse de la PE et de la P

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48

Chiu, May Leung. "Investigation and characterization of analyte-reporter enzyme conjugates for the enzyme-multiplied immunoassay technique." Diss., Restricted to subscribing institutions, 2009. http://proquest.umi.com/pqdweb?did=1930910331&sid=1&Fmt=2&clientId=1564&RQT=309&VName=PQD.

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49

Fedatto, Luciana Maria. "Caracterização de proteases extracelulares produzidas por Xylella fastidiosa de citros e videira." Universidade de São Paulo, 2005. http://www.teses.usp.br/teses/disponiveis/91/91131/tde-21062005-134055/.

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Xylella fastidiosa é uma bactéria patogênica encontrada em várias plantas. Esta bactéria secreta proteases extracelulares detectadas em gel de eletroforese, sendo a gelatina usada como substrato co-polimerizado. Três principais bandas protéicas foram detectadas com massa molar (MM) de 122, 84 e 65 kDa produzidas pelo isolado de citros (X0) e duas bandas de aproximadamente 84 e 65 kDa de isolado de videira (9713). Estas bactérias produziram zonas de hidrólise em meio sólido contendo gelatina, caseína e hemoglobina. Os resultados usando a gelatina como substrato foram os melhores para a atividad

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50

Preneta, A. Z. "Studies on lactate oxidising enzymes and their application to ferrocene-based enzyme electrodes for lactate." Thesis, Cranfield University, 1987. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.376191.

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