Bibliografías temáticas / Glycerin – Biotechnology

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Literatura académica sobre el tema "Glycerin – Biotechnology"

Autor: Grafiati
Publicado: 4 de junio de 2021
Última modificación: 10 de febrero de 2022

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Artículos de revistas sobre el tema "Glycerin – Biotechnology"

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Coombs, Amy. "Glycerin bioprocessing goes green". Nature Biotechnology 25, n.º 9 (septiembre de 2007): 953–54. http://dx.doi.org/10.1038/nbt0907-953.

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Santos, Willian Pereira, Beatriz Ferreira Carvalho, Carla Luiza Silva Ávila, Gilson Sebastião Dias Júnior, Marcos Neves Pereira y Rosane Freitas Schwan. "Glycerin as an additive for sugarcane silage". Annals of Microbiology 65, n.º 3 (29 de octubre de 2014): 1547–56. http://dx.doi.org/10.1007/s13213-014-0993-x.

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Sousa, Marylane, Juliana Rabelo Sousa, Vânia Maria Maciel Melo y Luciana Rocha Barros Gonçalves. "Screening of bacillus strains for biosurfactants production using glycerin as carbon source". Journal of Biotechnology 150 (noviembre de 2010): 288. http://dx.doi.org/10.1016/j.jbiotec.2010.09.229.

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da Silva, Sabrina Roberta Santana, Thayza Christina Montenegro Stamford, Wendell Wagner Campos Albuquerque, Esteban Espinosa Vidal y Tânia Lúcia Montenegro Stamford. "Reutilization of residual glycerin for the produce β-carotene by Rhodotorula minuta". Biotechnology Letters 42, n.º 3 (14 de enero de 2020): 437–43. http://dx.doi.org/10.1007/s10529-020-02790-8.

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Walter, Erhard y Peter Kohlerz. "Ringversuch f�r die enzymatische Bestimmung von Glycerin". Zeitschrift f�r Lebensmittel-Untersuchung und -Forschung 180, n.º 2 (febrero de 1985): 121–25. http://dx.doi.org/10.1007/bf01042635.

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Sousa, J. R., J. A. C. Correa, J. J. L. Martins, V. M. M. Melo, A. J. G. Cruz y L. R. B. Gonçalves. "A Kinetics Studies of the Production Rhamnolipids by Pseudomonas aeruginosa LAMI from Glycerin". Journal of Biotechnology 150 (noviembre de 2010): 395–96. http://dx.doi.org/10.1016/j.jbiotec.2010.09.510.

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Chiu, Chuang-Wei, Michael J. Goff y Galen J. Suppes. "Distribution of methanol and catalysts between biodiesel and glycerin phases". AIChE Journal 51, n.º 4 (2005): 1274–78. http://dx.doi.org/10.1002/aic.10385.

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Santo, Alessandra Argolo Espirito, Bethania Felix Ramos, Leila Cristiane De Souza, Marcelo Embiruçu De Souza, Milton Abreu Roque, José Assunção Ribeiro, Diene Oliveira Novais, Elias Ramos De Souza y Paulo Fernando Almeida. "Production of xanthan using sucrose, glycerin, vegetables leftovers in oil water effluent". Current Opinion in Biotechnology 22 (septiembre de 2011): S60—S61. http://dx.doi.org/10.1016/j.copbio.2011.05.169.

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Sattler, K. "Studien zur Intensivierung der Umwandlung von Glycerin in Dihydroxyaceton durch Acetobacter suboxydans". Zeitschrift für allgemeine Mikrobiologie 5, n.º 2 (24 de enero de 2007): 136–46. http://dx.doi.org/10.1002/jobm.19650050208.

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Kijeński, Jacek. "Biorefineries - from biofuels to the chemicalization of agricultural products". Polish Journal of Chemical Technology 9, n.º 3 (1 de enero de 2007): 42–45. http://dx.doi.org/10.2478/v10026-007-0051-6.

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Biorefineries - from biofuels to the chemicalization of agricultural products The recent research into the viable economy in sustainable energy from renewable sources has prompted a review into the potentials of Polish-oriented raw material sources as a catalyst for technological advance, product diversification and consumer satisfaction. The elongation of the process chain in vegetable (rape seed, potato), alcohol additives and glycerin processing has been found to drastically improve energy balances for the short processing methods adopted presently and can make Poland self sustainable in the future.
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Tesis sobre el tema "Glycerin – Biotechnology"

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Mafu, Lubabalo Rowan. "Benefication of glycerol from algae and vegetable oil". Thesis, Nelson Mandela Metropolitan University, 2011. http://hdl.handle.net/10948/d1011503.

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This research has been directed at furthering the utilization of crude glycerol oversupply formed as a by-product from the biodiesel manufacturing process. Phosphorylation of hydroxyl groups is a synthetic route that was investigated for the conversion of glycerol into a glycerol-phosphate (GPE) ester mixture. The process investigated for the synthesis of a GPE product was based on phosphorylation reaction procedures that were previously reported in the literature. The reaction to convert glycerol into a GPE mixture has been thoroughly investigated and the hydrogen chloride gas formed as a reaction by-product has been optimized. The chemical properties of GPE have been studied and discussed together with a mass balance of the overall glycerol phosphorylation process. The phosphate groups contained in polyhydric phosphate molecules have a potential chelating effect on cations. There are several cations that may be chelated by the phosphate ester group of polyhydric phosphate molecules. These cations include ammonium (NH4+), Potassium (K+), Calcium (Ca2+) etc, which are essential as nutrients in plant fertilizer formulations. This research has investigated the use of a GPE synthesized from glycerol in the laboratory and the use thereof as a phosphorus containing base in the formulation and evaluation of Nitrogen, Phosphorus and Potassium (NPK) containing fertilizer solution, Ammonium-Potassium-Glycerol-Phosphate (APGP) fertilizer solution. The APGP fertilizer solution has further been evaluated by growing two week old tomato seedlings under controlled conditions. The performance of the APGP fertiliser solution has been evaluated using design of experiments by comparison with traditionally used liquidAmmonium-Potassium-Phosphate inorganic fertilizer. This fertilizer solution has been prepared in similar manner as APGP formulation with the difference between them being the source of phosphorus. The results have been evaluated using statistic analysis where a significant difference between the evaluated fertilizer formulations was found. The comparative study of these formulations was monitored by the observed plant weights. A blank treatment was used as a control to determine if a significant difference among these formulations was observed. Anova single factor and t-Test methods (Two-Samples assumed of equal variances) are statistical models that were applied to interpret the observed experimental data with respect to wet and dry weighed masses of tomato seedlings. These methods indicated a confirmed conclusion that there was a significant difference between APPO4 solution and APGP solution. The observed data have shown that the APPO4 solution provided significantly better fertigation performance than APGP solution. Consequently, further investigation has been conducted to determine the cause of the poorer performance of the APGP solution. The further study of the APGP fertilizer solution included nutrient stability testing, biological analysis and other observed physical changes of the APGP solution over time. Biological results have revealed the presence of a Fusarium fungus species that has grown and is suspended in APGP fertilizer solution. This microbe species has been observed to play a vital role in consuming fertilizer nutrients. In addition, the observed abnormal plant growth and nutrient decomposition of the APGP formulation has been proposed to be mostly a result of the pathogenicity of the fusarium fungi species that was suspended in the APGP solution. Further work has been proposed in which the effect of such biological contamination is eliminated through adequate sterilization procedures and the APGP formulation re-evaluated.
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Andrade, Cristiano José de 1984. "Estudo da utilização do glicerol oriundo da cadeia do biodiesel por Bacillus subtilis para a produção de biossurfactantes, enzimas e aromas". [s.n.], 2011. http://repositorio.unicamp.br/jspui/handle/REPOSIP/256666.

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Orientador: Glaucia Maria Pastore
Dissertação (mestrado) - Universidade Estadual de Campinas, Faculdade de Engenharia de Alimentos
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Resumo: Os parâmetros ótimos de desodorização (temperatura, porcentagem de vapor e pressão de operação) são determinados de acordo com o tipo de óleo e o processo de refino selecionado. O refino do óleo de palma é realizado preferencialmente através do refino físico, pois sua elevada acidez pode provocar uma perda excessiva de óleo neutro no caso da utilização do refino químico. O refino físico de óleos vegetais consiste num processo de esgotamento ou stripping, no qual sob pressões reduzidas e elevadas temperaturas os compostos voláteis são removidos do óleo neutro através da utilização de um agente de arraste, que pode ser um gás ou vapor. No entanto, estas condições de processo também facilitam a ocorrência de volatilização de valiosos compostos minoritários como esqualeno, tocoferóis, tocotrienóis, fitoesteróis, bem como uma pequena porção dos acilgliceróis. Desta forma, este trabalho apresenta a aplicação da metodologia de superfície de resposta (MSR) no estudo dos efeitos de três variáveis de processo (temperatura, porcentagem de vapor e fração estearina/oleína), sobre a perda/transferência de compostos graxos durante a desacidificação física. As respostas de interesse foram acidez final do óleo e perda de óleo neutro (PON). Os resultados revelaram que a composição do óleo é um fator importante e estatisticamente significante a ser considerado na seleção de variáveis de processo mais adequadas, além da temperatura e da porcentagem de vapor. Na segunda etapa, foi realizado um estudo detalhado da degradação térmica dos carotenóides no óleo de palma considerando-se temperaturas variando na faixa de 170 ºC a 230 ºC. O processo de aquecimento foi realizado com injeção de nitrogênio e a coleta de amostra foi feita a cada 20 min durante um período de aquecimento total de 140 min. Os dados experimentais foram então comparados com dados da literatura relacionados à degradação de carotenóides. A degradação térmica dos carotenóides mostrou uma melhor adequação a uma ordem de reação superior a 1. Em uma etapa posterior, a metodologia de superfície de resposta (MSR) foi aplicada para o refino físico de amostras de óleo de palma de diferentes qualidades, medidas através de sua acidez inicial. Foram consideradas a influência de duas condições de operação e um terceiro parâmetro de qualidade, sendo estes: temperatura, porcentagem de vapor e acidez inicial das amostras. As respostas de interesse foram acidez final do óleo, retenção de tocóis, perda de óleo neutro (PON) e retenção de carotenóides. Os resultados mostraram que a acidez inicial é um parâmetro estatisticamente importante para a obtenção de valores aceitáveis de acidez final. Este parâmetro está diretamente relacionado à concentração de componentes minoritários no óleo, pois caso o valor de acidez final não tenha atendido aos padrões pré-determinados, o óleo deve ser submetido a tratamentos subseqüentes utilizando-se temperaturas mais elevadas ou maiores tempo de processo, portanto, acarretando a redução dos compostos minoritários. Para finalizar este trabalho foi avaliada a ocorrência da reação de hidrólise de óleo neutro durante o processo de desacidificação física. Os resultados revelaram que as maiores concentrações de ácidos graxos livres (AGL) no óleo e as menores concentrações de AGL no destilado foram obtidas para as maiores vazões de vapor e menores tempos de processo. Observou-se também, que a geração de AGL aumenta com o aumento da porcentagem de vapor e da temperatura do processo. Através da análise da cinética da hidrólise de óleo neutro, foi possível verificar uma boa adequação dos dados à equação de primeira-ordem
Abstract: Optimal deodorizing parameters (temperature, steam percentage, and operating pressure) are determined by the type of oil and the selected refining process. Refining of palm oil is preferably performed by physical means since its high acidity can lead to excessive losses of neutral oil in case of the caustic refining process. Physical refining of vegetable oils is a stripping process in which, under low absolute pressure and high temperatures, the accompanying lower boiling compounds are distilled off from neutral oil, by using gas or steam, as effective stripping agent. However, these processing conditions also increase the occurrence of volatilization of the valuable minor components present in the oil such as squalene, tocopherols, phytosterols, as well as some portion of acylglycerols. Thus, this work presents the application of response surface methodology (RSM) to the study of the effects of three factors (temperature, percentage of stripping steam and the stearin/olein ratio) on the losses/transfer of fatty compounds during the steam deacidification. The responses of interest were the final oil acidity and the loss of neutral oil (NOL). The results revealed that the oil composition was an important and statistically significant factor in the selection of more suitable processing variables, besides temperature and steam. In the second step, a detailed study was performed for carotene thermal degradation in palm oil at four temperatures ranging from 170 ºC to 230 ºC. The heating process was carried out with injection of nitrogen and the samples were collected every 20 min during a total heating period of 140 min. The experimental data were then compared to literature data concerning carotenoids thermal degradation. The thermal degradation kinetics of carotenoids in palm oil followed an order superior to 1. In the next step of this work, response surface methodology (RSM) was applied to study the physical refining process of palm oil samples of different qualities, measured by their initial acidity. The influence of two operative conditions and a third quality parameter are considered: the temperature, the steam percentage, and the initial FFA. Their effect on the final FFA, tocols retention, neutral oil loss (NOL), and carotene retention was investigated. The results revealed that the initial FFA is a statistically important parameter to obtain acceptable values for final FFA, which has directly relation with the concentration of minor components in the oil by the submission to higher temperatures or longer refining periods, in order to reach the standard values. To end this work, it was evaluated the occurrence of hydrolysis of neutral oil during the steam deacidification. The results revealed that the highest percentages of FFA into the oil and the lowest percentages of FFA on the distillate were obtained when applying high steam flow rates associated with reduced stripping times. It was also observed that the generation of free fatty acids increases with the increasing of the percent of steam injected into the oil as well as with the increasing of the process temperature. The kinetic investigation revealed that the hydrolysis of neutral oil follows a first-order reaction
Mestrado
Ciência de Alimentos
Mestre em Ciência de Alimentos
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Coêlho, Dayana de Gusmão 1986. "Modelagem e otimização do processo de síntese do ácido propanóico via fermentação do glicerol". [s.n.], 2011. http://repositorio.unicamp.br/jspui/handle/REPOSIP/266877.

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Orientador: Rubens Maciel Filho
Dissertação (mestrado) - Universidade Estadual de Campinas, Faculdade de Engenharia Química
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Resumo: Atualmente, a grande parcela de produção do ácido propanóico ocorre a partir da síntese química, tendo como matéria-prima o petróleo. O conhecimento das limitações das fontes não-renováveis, sobretudo o esgotamento das matérias derivadas do petróleo, constitui o grande desafio na busca de fontes alternativas, competitivas e sustentáveis, em face do intento na preservação ambiental. O presente trabalho versa acerca do estudo de processo fermentativo, visando a minimizar a exploração desenfreada dos recursos naturais, comandada atualmente pelas fontes petroquímicas, bem como propiciar caminhos paralelos e alternativos por meio de fontes renováveis. Desta forma, este projeto tem como objetivo investigar a produção do ácido propanóico, por meio de processo biotecnológico, utilizando como matéria-prima o glicerol e o microrganismo propionibacterium acidipropionici. Neste âmbito, para a análise do sistema em batelada, será realizada a modelagem matemática do processo, por intermédio de modelos não estruturados, e a simulação do referido processo pelo método de Runge Kutta. Os parâmetros operacionais e cinéticos foram otimizados por intermédio da aplicação da técnica de planejamentos de experimentos por Metodologia de Superfície de Resposta e pelo Algoritmo Genético. Deste modo, por meio de desenvolvimento dos modelos, otimização dos parâmetros e das condições de operação podemos, determinar as condições, limitações e viabilidade do processo constituindo ferramentas fundamentais na investigação do comportamento do processo a fim de maximizar a produção do ácido propanóico possibilitando uma rota alternativa para este processo
Abstract: Currently, a large proportion of propionic acid production occurs from chemical synthesis, with the raw oil. Knowing the limitations of non-renewable sources, especially the depletion of petroleum derived feedstock, is the great challenge in finding alternative sources, competitive and sustainable in the face of intent on environmental preservation. The present paper is about the study of the fermentation process, in order to minimize the uncontrolled exploitation of natural resources, currently led by petrochemical sources, as well as provide alternative and parallel paths through renewable sources. Thus, this project aims to investigate the production of propionic acid by biotechnology process using as raw glycerol and microorganism Propionibacterium acidipropionici. In this context, to analyze the batch system will be the mathematical model of the process through unstructured models, and simulation of this process by the Runge Kutta. The operational parameters and kinetics were optimized through the application of the technique of planning of experiments by Response Surface Methodology and the Genetic Algorithm. Thus, through the development of models, optimization of parameters and operating conditions can determine the conditions, limitations and feasibility of the process constitutes the fundamental tools for investigating the behavior of the process in order to maximize production of propionic acid providing an alternative route for this process
Mestrado
Desenvolvimento de Processos Químicos
Mestre em Engenharia Química
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Powtongsook, Sorawit. "Physiology and biotechnology of glycerol production using the green microalga Dunaliella". Thesis, University of Sheffield, 1998. http://etheses.whiterose.ac.uk/15086/.

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Sun, Chenhao. "Dynamic metabolic studies of C. necator producing PHB from glycerol". Thesis, University of Manchester, 2018. https://www.research.manchester.ac.uk/portal/en/theses/dynamic-metabolic-studies-of-c-necator-producing-phb-from-glycerol(4b627cd4-c6c9-4f2c-9a2a-2e3da8d4f68a).html.

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The development of human society, which is highly dependent on fossil fuels, is now facing a range of global issues, such as rising energy prices, energy security and climate changes. To successfully tackle the resultant issues, the energy transition from fossil fuels to renewable energy sources, such as solar energy, tide energy, hydroelectric power, geothermal heat and biofuels, is under way. Biodiesel, as an important type of biofuels, has been increasingly produced from vegetable oil or used cooking oil, especially in Europe. Nevertheless, considering the high production cost of biodiesel, there is still much to be done to improve the economics of biodiesel industry. Utilisation of crude glycerol, the main by-product of the biodiesel industry, to produce value-added products appears to be a promising solution. Poly(3-hydroxybutyric acid) (PHB), a biodegradable plastic, can be converted from glycerol by Cupriavidus necator DSM 545 under unbalanced growth conditions, such as nitrogen limitation. One way to enhance the batch production of PHB is to genetically engineer the strain of C. necator, which requires insights of the dynamic impact of extracellular environment on cell phenotypes. Hence in this thesis, we aim to perform metabolic modelling based on experimental measurements to gain a better understanding of the behaviour of the metabolic network of Cupriavidus necator DSM 545 and identify potential bottlenecks of the process. Initially, C. necator DSM 545 is a strain that hardly grows on glycerol, so in a preliminary study, we investigate the process by which the strain was adapted to consume glycerol through serial subcultivation. It is found that the adaptation can be achieved within 15 cell generations over three passages in basal mineral medium, and the acquired phenotype is sufficiently stable upon further passage. The study of metabolism started with the reconstruction of the cell's metabolic network, followed by a thermodynamic analysis to check the feasibility and reversibility of all the biochemical reactions included. Then the static flux balance analysis was extended and applied to analyse the shift of metabolic states during the microbial fermentation in different batch conditions. The resulting patterns of flux distribution reveal the TCA cycle to be the major competitor for PHB synthesis at the ACCoA node. Cells have the potential to enter an efficient PHB-production phase that features minimal TCA/PHB flux split ratio, and the length of the phase can be manipulated by aeration. Although low aeration rate favours optimal flux split ratio, such condition that limits respiration also limits nutrient uptake, leading to low PHB productivity overall. To identify the actual limiting factors of PHB synthesis in the system, we further performed metabolic control analysis based on the calculated flux distributions. The analysis demonstrated how the distribution of the metabolic control can vary widely, depending on the aeration conditions used and the flux split ratios. Glycerolipid pathway, glycolysis, PHB metabolism, as well as the electron transport chain are revealed to be potential engineering targets as they contribute to the great majority of the positive control of PHB flux.
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Bian, Lei. "An in vitro antimicrobial and safety study of Lactobacillus reuteri DPC16 for validation of probiotic concept : a thesis presented in partial fulfilment of the requirements for the degree of Master of Technology in Biotechnology at Massey University, Auckland, New Zealand". Massey University, 2008. http://hdl.handle.net/10179/830.

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Based on previous studies of the novel Lactobacillus reuteri DPC16 strain, an in vitro investigation on the supernatant antimicrobial activity and the culture safety against normal gastrointestinal microflora and gastric mucus was done in this thesis. DPC16 cell-free supernatants (fresh and freeze-dried, designated as MRSc and FZMRSc) from anaerobic incubations in pre-reduced MRS broth, have shown significant inhibitory effects against selected pathogens, including Salmonella Typhimurium, E. coli O157:H7, Staphylococcus aureus, and Listeria monocytogenes. These effects were mainly due to the acid production during incubation as evidenced by the negation of such activity from their pH-neutral counterparts, and this acidic effect was shown to reduce the pathogen growth rate and decrease the total number of pathogen cells. By incubation of concentrated (11 g/L) resting cells in glycerol-supplemented MRS broth, another DPC16 cell-free supernatant (designated as MRSg) has shown very strong antimicrobial effect against all target pathogens. As indicated by a kinetic profile, this activity developed in a sigmoidal fashion as incubation proceeded, reaching to maximum activity after 6-8h and maintained at the same level thereafter. Further study has shown that the antimicrobial activity of this supernatant was pH-independent, effective across a pH range of 4.6 to 6.5, and acted on both Gram-negative and Gram-positive pathogens. Using the minimum effective dose, a time course investigation has provided evidence that this supernatant affected the growth of the target pathogens by elongating the lag phase and lowering the total cell number at the end of the incubation. Lastly, it was found that the strong antimicrobial effect of MRSg was bactericidal at high concentrations and bacteriostatic at low concentrations. However, it also found that the viability of DPC16 cells also decreased as incubation prolonged, which suggests that this glycerol-derived supernatant had a lethal effect to its own cells. Nevertheless, this lethal effect was exerted to a much lesser extent compared with that to the pathogens. The last DPC16 cell-free supernatant was designated as SGF, which was produced from secondary fermentation of the same resting cells in glycerol-water. SGF did not show a significant antimicrobial activity, which suggests that this specific strain is not capable of utilising glycerol in the absence of fermentable carbohydrates. The antimicrobial activity found in MRSg matched with previously published characteristics of reuterin, which is a unique antimicrobial substance synthesised by L. reuteri when incubated with glycerol. Therefore, a study on the production kinetics of reuterin by DPC16 was carried out. Supernatants of both MRSg and SGF were studied. Results showed that glycerol utilisation occurred in both MRSg and SGF, whereas the bioconversion of glycerol into reuterin was different. In MRSg, glycerol was constantly utilised by DPC16 resting cells, and by the end of an 18h incubation 85.8 mM of glycerol was utilised, where 72.8% was transformed into reuterin. The formation of reuterin initiated with an inclining production and reached the maximum rate of 10.9 mM/h after 6h of incubation, with the total production of 64 mM of reuterin at the end of the 18h incubation. This reuterin production in MRSg followed a similar pattern to that of its antimicrobial activity, which suggests a certain correlation between reuterin formation and the increase of antimicrobial activity in MRSg. Therefore, the major antimicrobial component in MRSg that accounted for its potent antimicrobial activity was very much likely to be reuterin. In SGF, however, detectable reuterin was negligible even though some glycerol may have been absorbed into the highly concentrated DPC16 resting cells. This has responded to the antimicrobial activity assay in that due to the lack of essential carbohydrate nutrient for normal cell metabolism, there was no glycerol utilisation and hence no reuterin synthesis. Having studied the antimicrobial activity of L. reuteri DPC16 and the production of antimicrobial-competent reuterin, two safety issues (the impact on growth on other normal commensal probiotics and mucin degradation activity) of this strain were assessed to further evaluate its probiotic potential. By using similar in vitro assays as in the antimicrobial test, the same set of DPC16 supernatants have demonstrated no adverse effect on the growth of either Lactobacillus acidophilus, Lactobacillus plantarum, Pediococcus acidilactici, or Bifidobacterium lactis DR10. No stimulatory effect was found either. By incorporating purified porcine gastric mucin into classic mucin-degradation assays in both liquid and agar media, DPC16 has not exhibited the same mucinolytic activity as that of the faecal flora cultures. Thus, it can be concluded that L. reuteri DPC16 is as safe to the host as normal commensal microflora.
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Ingfeldt, Isac. "Evaluation of Carbon Source Addition on Denitrification Efficiency : A study in a continuous biological leachate water treatment system". Thesis, KTH, Skolan för kemi, bioteknologi och hälsa (CBH), 2020. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-278587.

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In 2014 SÖRAB constructed a continuous biological treatment system (KBR) to handle leachate waterfrom the landfill at the facility in Löt, north of Stockholm. The KBR is mainly focused on removal ofammonium nitrogen which would otherwise be released in to the recipient and contribute toeutrophication and damage to the environment. This project has focused on replacing the currentcarbon source in the process Brenntaplus VP1 and evaluating the efficiency of denitrification andeconomy of transitioning to a new carbon source. The carbon sources glycerol and ethanol wereevaluated and compared to Brenntaplus VP1 for the denitrification efficiency and microbial profile.The experiments were performed in laboratory conditions and in pilot scale using leachate water fromLöt. The reduction of ammonia was evaluated by chemical precipitation, addition of carbon sources bymeasuring ammonia-N and nitrate-N under aerobic (nitrification) and anaerobic (denitrification)conditions. The combination of ethanol and glycerol showed an enhanced denitrification and increasedmicrobial community both in lab and pilot scale studies with reduced hydraulic retention time. Therate of nitrate reduction was 0.23 mgNO3-N 1 -1 h -1 for ethanol/glycerol compared to 0.12-0.17mgNO 3- -N 1 -1 h -1 for Brenntaplus VP1 in pilot scale. The results indicate that using ethanol, glycerolor a mix of the two as a substitute for Brenntaplus VP1 is viable. This has been based on laboratoryand pilot scale studies. Each of the carbon sources examined during this project have showed a uniqueimpact on the process and its parameters such as: denitrification rate, microbial density and microbialcomposition. The carbon sources had an impact with temperature fluctuation and faster denitrificationcompared to the conventional KBR system. This implies that the carbon sources tested in this projectcan be advantageous and beneficial for Sörab depending on the carbon source availability and theseasonal variations.
Under 2014 konstruerade SÖRAB ett kontinuerligt biologiskt reningsverk (KBR) för att hanteralakvatten från deponin för ickefarligt avfall vid anläggningen i Löt, norr om Stockholm. KBR ärfrämst konstruerad för rening av ammoniumkväve som annars skulle släppas ut till recipienten ochbidra till övergödning och skador på miljön i området. Detta projekt har fokuserat på att ersätta dennuvarande kolkällan Brenntaplus VP1 som används i processen och utvärdera effektiviteten idenitrifieringen samt ekonomin vid övergång till en ny kolkälla. Kolkällorna glycerol och etanol varde kolkällor som valdes för utvärdering i detta projekt, dessa jämfördes med Brenntaplus VP1 i desseffekt på denitrifikationseffektivitet och mikrobiell sammansättning under laboratorieförhållanden ochi pilotskala. Möjligheten att reducera ammoniumkoncentrationen i lakvattnet utvärderades genomkemisk fällning och genom mätning av ammoniumkväve och nitratkväve under aeroba (nitrifikation)och anaeroba (denitrifikation) förhållanden. Kombinationen av etanol och glycerol indikerade enförbättrad denitrifikation och ökad mikrobiell densitet både i laboratorie- och pilotskala med reduceradhydraulisk retentionstid. Nitratreduktionshastigheten var 0,23 mgNO 3- -N 1 -1 h -1 för blandningen avetanol/glycerol jämfört med 0,12 - 0,17 mgNO 3- -N 1 -1 h -1 för Brenntaplus VP1 i pilotskala. Resultatenindikerar att användning av etanol, glycerol eller en blandning av de två har goda förutsättningar föratt ersätta Brenntaplus VP1. Var och en av de tre kolkällorna som undersöktes under detta projekt harvisat en unik inverkan på processen och dess parametrar såsom: denitrifikationshastighet, mikrobielldensitet och mikrobiell sammansättning. Genom att byta kolkälla i KBR kan prestandan ökas genomatt minska den hydrauliska retentionstiden samtidigt som systemet tycks bli mindre känsligt förtemperatursvängningar. Kolkällorna som utvärderats i detta projekt kan därför vara fördelaktiga för SÖRAB beroende på dess tillgänglighet och pris.
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PRADINES, ANTOINE. "Phosphorylation enzymatique de polyols par la phosphatase alcaline optimisation de la synthese de glycerol-1-phosphate". Toulouse 3, 1989. http://www.theses.fr/1989TOU30015.

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Mise au point et optimisation d'une nouvelle methode de phosphorylation enzymatique, utilisant la phosphatase alcaline. Cette methode permet la monophosphorylation de nombreux derives mono ou polyhydroxyles avec des rendements et une regioselectivite eleves. Pour ce faire, un bioreacteur enzymatique a ete utilise contenant l'enzyme immobilise sur support
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El-Shetehy, Mohamed H. "Molecular and Biochemical Signaling Underlying Arabidopsis-Bacterial/Virus/Fungal Interactions". UKnowledge, 2016. http://uknowledge.uky.edu/plantpath_etds/19.

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Systemic acquired resistance (SAR) is a form of inducible defense response triggered upon localized infection that confers broad-spectrum disease resistance against secondary infections. Several factors are known to regulate SAR and these include phenolic phytohormone salicylic acid (SA), phosphorylated sugar glycerol-3-phosphate (G3P), and dicarboxylic acid azelaic acid (AzA). This study evaluated a role for free radicals nitric oxide (NO) and reactive oxygen species (ROS) in SAR. Normal accumulation of both NO and ROS was required for normal SAR and mutations preventing NO/ROS accumulation and/or biosynthesis compromised SAR. A role for NO and ROS was further established using pharmacological approaches. Notably, both NO and ROS conferred SAR in a concentration dependent manner. This was further established using genetic mutants that accumulated high levels of NO. NO/ROS acted upstream of G3P and in parallel to SA. Collectively, these results suggest that NO and ROS are essential components of the SAR pathway.
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Bianchi, Ivan. "Congelamento de sêmen suíno: estudo de crioprotetores intra e extracelulares, metodologias de congelamento e marcador molecular de congelabilidade". Universidade Federal de Pelotas, 2007. http://guaiaca.ufpel.edu.br/handle/123456789/1229.

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Made available in DSpace on 2014-08-20T13:32:49Z (GMT). No. of bitstreams: 1 tese_ivan_bianchi.pdf: 495717 bytes, checksum: 66978c47d9c193a45f85b5cad5be6c3f (MD5) Previous issue date: 2007-02-27
Although boar semen is available in the frozen format since 1975, its use has been restricted to very specific situations for many reasons: it requires spermatozoa concentration per dose twice to three times higher than that for cooled semen; semen processing is labor intensive; and farrowing rate and litter size are reduced. Thus, most of the artificial inseminations in swine are performed at the day of semen collection or at most at the following day, using liquid semen conditioned between 15 and 18 ºC. The aims of this thesis were: to evaluate distinct extenders for cooling semen, methods for freezing, centrifugation temperatures, the use of amides as penetrating cryoprotectants and of low density lipoprotein as nonpenetrating cryoprotectants, and the presence of specific protein factors in seminal plasma in the results that could be associated with boar semen freezability. Five experiments have been executed: four with evaluations in vitro (motility and cell membrane integrity); and one with fertilization in vivo. The control treatment was the freezing with glycerol at 3% concentration. The semen processing method that provide more efficient results consisted of semen cooling during 120 min up to a centrifugation temperature of 15 °C. We also identified a 26 kDa factor in the seminal plasma that is associated with maintenance of the integrity of the sperm cell membrane post-thawing. Additionally, parameters of semen quality in vitro with the use of dimetilacetamide at 5% were better than those observed with the most used penetrating cryoprotectant (glycerol 3%) as control, although no difference between those cryoprotectants was observed in vivo. This study demonstrated the association of components in the seminal plasma with the sperm quality, and presenting an alternative protocol of semen freezing-thawed boar semen based in the use of dimetilacetamide at 5%.
Apesar do sêmen suíno congelado estar disponível comercialmente deste 1975, o seu uso tem ocorrido somente em ocasiões específicas, pois, em relação ao uso de sêmen refrigerado, requer duas a três vezes maior número de espermatozóides por dose, o processamento do sêmen é trabalhoso, o tamanho da leitegada é diminuído em um a três leitões por parto e a taxa de parição é menor. Dessa forma, a grande maioria das inseminações artificiais nessa espécie utiliza sêmen diluído e acondicionado no estado líquido entre 15 e 18 ºC. Os objetivos desta tese foram: avaliar diferentes diluidores de resfriamento, metodologias de congelamento, temperaturas de centrifugação, uso de crioprotetores intracelulares a base de amidas e extracelulares baseados em lipoproteína de baixa densidade, além do estudo de fatores protéicos presentes no plasma seminal nos resultados de avaliações in vitro e in vivo de sêmen suíno congelado-descongelado. Foram executados cinco experimentos com avaliações da qualidade do sêmen congeladodescongelado, quatro experimentos com avaliações in vitro (motilidade e integridade de membrana) e um experimento com fertilização in vivo. O tratamento utilizado como controle foi o congelamento com glicerol na concentração de 3%. A melhor curva de resfriamento após a coleta até 15 °C, foi atingida com o tempo de 120 min. Foi identificado um fator de 26 kDa no plasma seminal, cuja ausência proporcionou melhores resultados de integridade de membrana plasmática após o descongelamento. O uso do crioprotetor intracelular dimetilacetamida na concentração de 5%, proporcionou nas avaliações in vitro, resultados superiores ao tratamento controle (glicerol 3%), não diferindo na avaliação in vivo. O presente estudo demonstrou a relação de componentes do plasma seminal com a qualidade espermática, além de apresentar um novo protocolo de congelamentodescongelamento de sêmen suíno baseado no uso de dimetilacetamida 5%.
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Libros sobre el tema "Glycerin – Biotechnology"

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George, Aggelis, ed. Microbial conversions of raw glycerol. New York: Nova Biomedical Books, 2009.

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George, Aggelis, ed. Microbial conversions of raw glycerol. New York: Nova Biomedical Books, 2009.

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George, Aggelis, ed. Microbial conversions of raw glycerol. New York: Nova Biomedical Books, 2009.

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George, Aggelis, ed. Microbial conversions of raw glycerol. New York: Nova Biomedical Books, 2009.

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Capítulos de libros sobre el tema "Glycerin – Biotechnology"

1

Semkiv, Marta, Kostyantyn Dmytruk, Charles Abbas y Andriy Sibirny. "Biotechnology of Glycerol Production and Conversion in Yeasts". En Biotechnology of Yeasts and Filamentous Fungi, 117–48. Cham: Springer International Publishing, 2017. http://dx.doi.org/10.1007/978-3-319-58829-2_5.

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Gong, Cheng S., Jiau X. Du, Ning J. Cao y George T. Tsao. "Coproduction of Ethanol and Glycerol". En Twenty-First Symposium on Biotechnology for Fuels and Chemicals, 543–59. Totowa, NJ: Humana Press, 2000. http://dx.doi.org/10.1007/978-1-4612-1392-5_43.

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Huang, Wei-Cho, Cheng S. Gong y George T. Tsao. "Enhancement of Oxygen Transfer by Pressure Pulsation in Aqueous Glycerol Fermentation". En Biotechnology for Fuels and Chemicals, 909–20. Totowa, NJ: Humana Press, 2002. http://dx.doi.org/10.1007/978-1-4612-0119-9_74.

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Kumar, Prasun, Sanjeet Mehariya, Subhasree Ray, Anjali Mishra y Vipin Chandra Kalia. "Biotechnology in Aid of Biodiesel Industry Effluent (Glycerol): Biofuels and Bioplastics". En Microbial Factories, 105–19. New Delhi: Springer India, 2015. http://dx.doi.org/10.1007/978-81-322-2598-0_7.

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Ryu, Hwa-Won, Kui-Hyun Kang y Jong-Sun Yun. "Bioconversion of Fumarate to Succinate Using Glycerol as a Carbon Source". En Twentieth Symposium on Biotechnology for Fuels and Chemicals, 511–20. Totowa, NJ: Humana Press, 1999. http://dx.doi.org/10.1007/978-1-4612-1604-9_46.

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Ciapina, Elisa M. P., Walber C. Melo, Lidia M. M. Santa Anna, Alexandre S. Santos, Denise M. G. Freire y Nei Pereira. "Biosurfactant Production by Rhodococcus erythropolis Grown on Glycerol As Sole Carbon Source". En Twenty-Seventh Symposium on Biotechnology for Fuels and Chemicals, 880–86. Totowa, NJ: Humana Press, 2006. http://dx.doi.org/10.1007/978-1-59745-268-7_72.

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Wei, Yonghua, Cong Wang, Xiaohong Cao y Lihua Hou. "Effect of Gene FPS1 on Accumulation of Glycerol in Zygosaccharomyces rouxii". En Proceedings of the 2012 International Conference on Applied Biotechnology (ICAB 2012), 251–57. Berlin, Heidelberg: Springer Berlin Heidelberg, 2013. http://dx.doi.org/10.1007/978-3-642-37916-1_26.

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Ochoa, Jose Luis, Martin Ramírez-Orozco y Facundo Márquez. "Glycerol and Glucose Dissimilation by Debaryomyces hansenii: Substrate Influence on Growth Rate and Biomass Yield of Yeast". En Non-Conventional Yeasts in Genetics, Biochemistry and Biotechnology, 51–57. Berlin, Heidelberg: Springer Berlin Heidelberg, 2003. http://dx.doi.org/10.1007/978-3-642-55758-3_9.

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Petrovič, Uroš y Ana Plemenitaš. "Determination of Intra- and Extracellular Concentration of Glycerol in the Halophilic Black Yeast Hortaea werneckii Grown at Different Environmental Salinities". En Non-Conventional Yeasts in Genetics, Biochemistry and Biotechnology, 127–30. Berlin, Heidelberg: Springer Berlin Heidelberg, 2003. http://dx.doi.org/10.1007/978-3-642-55758-3_19.

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Yu, Jingjing, Jian Dong, Cuiying Zhang, Junxia Li y Dongguang Xiao. "Effect of GPD1 and GPD2 Deletion on the Production of Glycerol and Ethanol in the Yeast Saccharomyces cerevisiae". En Proceedings of the 2012 International Conference on Applied Biotechnology (ICAB 2012), 171–79. Berlin, Heidelberg: Springer Berlin Heidelberg, 2013. http://dx.doi.org/10.1007/978-3-642-37916-1_18.

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Actas de conferencias sobre el tema "Glycerin – Biotechnology"

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Rywińska, Anita, Ludwika Tomaszewska, Monika Bąk, Aleksandra Mirończuk, Krzysztof Cybulski y Waldemar Rymowicz. "Erythritol production from glycerol by Yarrowia lipolytica in batch, fed-batch and repeated-batch regimes". En Annual International Conference on Advances in Biotechnology. Global Science & Technology Forum (GSTF), 2013. http://dx.doi.org/10.5176/2251-2489_biotech13.62.

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Rymowicz, Waldemar, Agnieszka Kita, Anita Rywińska y Aleksandra Mirończuk. "Enhanced erythritol biosynthesis from glycerol by Yarrowia lipolytica in fed-batch processes". En 3rd Annual International Conference on Advances in Biotechnology (BioTech 2013). Global Science and Technology Forum, 2013. http://dx.doi.org/10.5176/2251-2489_biotech13.64.

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Tomaszewska, Ludwika, Anita Rywińska y Waldemar Rymowicz. "The effect of Zn, Mn and Cu ions on erythritol production from glycerol by Yarrowia lipolytica yeast". En Annual International Conference on Advances in Biotechnology. Global Science & Technology Forum (GSTF), 2013. http://dx.doi.org/10.5176/2251-2489_biotech13.61.

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Xue, Dan, Wei Lv y Heng Zhang. "Telechelic diols from polyhydroxybutyrate via alcoholysis with ethylene glycol or glycerol". En 2015 2nd International Conference on Machinery, Materials Engineering, Chemical Engineering and Biotechnology. Paris, France: Atlantis Press, 2016. http://dx.doi.org/10.2991/mmeceb-15.2016.51.

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Jacome, Alfredo Diaz y Marco Sanjuan Mejia. "A General Methodology for Optimized Takagi-Sugeno Fuzzy Modeling of Nonlinear Continuous Fermenter for Biofuels (Ethanol) Production Using Golden Section Search Approach". En ASME 2014 8th International Conference on Energy Sustainability collocated with the ASME 2014 12th International Conference on Fuel Cell Science, Engineering and Technology. American Society of Mechanical Engineers, 2014. http://dx.doi.org/10.1115/es2014-6720.

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The overcoming inclusion of biotechnology in biofuels industry involves several challenges among which are found the variety of operational cycles, the highly nonlinear behavior of the processes and the need for measurement of intermediate variables. In order to reproduce biological conversion of biodiesel production discharge products into other biofuels, experimental data from ethanol production from glycerol/glucose mixture was analyzed implementing fuzzy techniques to investigate and model the nonlinear behavior of the process. This paper presents a general methodology for TS fuzzy modeling based on a novel approach on data structured regression which consists on combination of fuzzy c-regression model and clustering using a golden search algorithm approach to adjust the proper number of membership functions to fit the model and minimize the statistic difference among the experimental data, simulated data and the Fuzzy Inference System results.
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