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Artículos de revistas sobre el tema "Glycerin – Biotechnology"

Autor: Grafiati
Publicado: 4 de junio de 2021
Última modificación: 10 de febrero de 2022

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1

Coombs, Amy. "Glycerin bioprocessing goes green." Nature Biotechnology 25, no. 9 (2007): 953–54. http://dx.doi.org/10.1038/nbt0907-953.

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Santos, Willian Pereira, Beatriz Ferreira Carvalho, Carla Luiza Silva Ávila, Gilson Sebastião Dias Júnior, Marcos Neves Pereira, and Rosane Freitas Schwan. "Glycerin as an additive for sugarcane silage." Annals of Microbiology 65, no. 3 (2014): 1547–56. http://dx.doi.org/10.1007/s13213-014-0993-x.

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3

Sousa, Marylane, Juliana Rabelo Sousa, Vânia Maria Maciel Melo, and Luciana Rocha Barros Gonçalves. "Screening of bacillus strains for biosurfactants production using glycerin as carbon source." Journal of Biotechnology 150 (November 2010): 288. http://dx.doi.org/10.1016/j.jbiotec.2010.09.229.

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4

da Silva, Sabrina Roberta Santana, Thayza Christina Montenegro Stamford, Wendell Wagner Campos Albuquerque, Esteban Espinosa Vidal та Tânia Lúcia Montenegro Stamford. "Reutilization of residual glycerin for the produce β-carotene by Rhodotorula minuta". Biotechnology Letters 42, № 3 (2020): 437–43. http://dx.doi.org/10.1007/s10529-020-02790-8.

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5

Walter, Erhard, and Peter Kohlerz. "Ringversuch f�r die enzymatische Bestimmung von Glycerin." Zeitschrift f�r Lebensmittel-Untersuchung und -Forschung 180, no. 2 (1985): 121–25. http://dx.doi.org/10.1007/bf01042635.

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6

Sousa, J. R., J. A. C. Correa, J. J. L. Martins, V. M. M. Melo, A. J. G. Cruz, and L. R. B. Gonçalves. "A Kinetics Studies of the Production Rhamnolipids by Pseudomonas aeruginosa LAMI from Glycerin." Journal of Biotechnology 150 (November 2010): 395–96. http://dx.doi.org/10.1016/j.jbiotec.2010.09.510.

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7

Chiu, Chuang-Wei, Michael J. Goff, and Galen J. Suppes. "Distribution of methanol and catalysts between biodiesel and glycerin phases." AIChE Journal 51, no. 4 (2005): 1274–78. http://dx.doi.org/10.1002/aic.10385.

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8

Santo, Alessandra Argolo Espirito, Bethania Felix Ramos, Leila Cristiane De Souza, et al. "Production of xanthan using sucrose, glycerin, vegetables leftovers in oil water effluent." Current Opinion in Biotechnology 22 (September 2011): S60—S61. http://dx.doi.org/10.1016/j.copbio.2011.05.169.

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9

Sattler, K. "Studien zur Intensivierung der Umwandlung von Glycerin in Dihydroxyaceton durch Acetobacter suboxydans." Zeitschrift für allgemeine Mikrobiologie 5, no. 2 (2007): 136–46. http://dx.doi.org/10.1002/jobm.19650050208.

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10

Kijeński, Jacek. "Biorefineries - from biofuels to the chemicalization of agricultural products." Polish Journal of Chemical Technology 9, no. 3 (2007): 42–45. http://dx.doi.org/10.2478/v10026-007-0051-6.

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Biorefineries - from biofuels to the chemicalization of agricultural products The recent research into the viable economy in sustainable energy from renewable sources has prompted a review into the potentials of Polish-oriented raw material sources as a catalyst for technological advance, product diversification and consumer satisfaction. The elongation of the process chain in vegetable (rape seed, potato), alcohol additives and glycerin processing has been found to drastically improve energy balances for the short processing methods adopted presently and can make Poland self sustainable in th
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11

Lovato, Giovanna, Roberta Albanez, Lucas Stracieri, Letícia Scudeler Ruggero, Suzana Maria Ratusznei, and José Alberto Domingues Rodrigues. "Hydrogen production by co-digesting cheese whey and glycerin in an AnSBBR: Temperature effect." Biochemical Engineering Journal 138 (October 2018): 81–90. http://dx.doi.org/10.1016/j.bej.2018.07.007.

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12

Wang, Xinyu, Shuguang Li, Hailiang Chen, Qiang Liu, Guangyao Wang, and Yunyan Zhao. "Compatibility of Temperature Sensor and Polarization Filter Based on Au Film and Glycerin Selectively Infilling Photonic Crystal Fibers." Plasmonics 11, no. 5 (2016): 1265–71. http://dx.doi.org/10.1007/s11468-015-0170-5.

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13

Triyono, Joko, Raasyid Alfiansyah, Heru Sukanto, Dody Ariawan, and Yohanes Nugroho. "Fabrication and characterization of porous bone scaffold of bovine hydroxyapatite-glycerin by 3D printing technology." Bioprinting 18 (June 2020): e00078. http://dx.doi.org/10.1016/j.bprint.2020.e00078.

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14

Yanagiya, Shin-ichiro, Aiko Takahashi, and Nobuo Goto. "Mechanical Properties of Stratum Corneum in Glycerin Solution by Atomic Force Microscopy." e-Journal of Surface Science and Nanotechnology 13 (2015): 461–64. http://dx.doi.org/10.1380/ejssnt.2015.461.

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15

Rodríguez-Abalde, Á., M. Guivernau, F. X. Prenafeta-Boldú, X. Flotats, and B. Fernández. "Characterization of microbial community dynamics during the anaerobic co-digestion of thermally pre-treated slaughterhouse wastes with glycerin addition." Bioprocess and Biosystems Engineering 42, no. 7 (2019): 1175–84. http://dx.doi.org/10.1007/s00449-019-02115-8.

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16

Hirokado, Rina, Seiji Noma, Nobuaki Soh, Noriyuki Igura, Mitsuya Shimoda, and Nobuyuki Hayashi. "Inactivation of Bacillus subtilis Spores by Carbonation with Glycerin Fatty Acid Esters." Food Science and Technology Research 24, no. 3 (2018): 455–63. http://dx.doi.org/10.3136/fstr.24.455.

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17

Lovato, Giovanna, Lia P. P. Batista, Marina B. Preite, et al. "Viability of Using Glycerin as a Co-substrate in Anaerobic Digestion of Sugarcane Stillage (Vinasse): Effect of Diversified Operational Strategies." Applied Biochemistry and Biotechnology 188, no. 3 (2019): 720–40. http://dx.doi.org/10.1007/s12010-019-02950-1.

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18

Murakami, Takahide, Ichiro Hijikuro, Kota Yamashita, et al. "Antiadhesion effect of the C17 glycerin ester of isoprenoid-type lipid forming a nonlamellar liquid crystal." Acta Biomaterialia 84 (January 2019): 257–67. http://dx.doi.org/10.1016/j.actbio.2018.12.009.

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19

Lovato, Giovanna, Roberta Albanez, Letícia Scudeler Ruggero, Lucas Stracieri, Suzana Maria Ratusznei, and José Alberto Domingues Rodrigues. "Energetic feasibility of a two-stage anaerobic digestion system compared to a single-stage system treating whey and glycerin." Biochemical Engineering Journal 161 (September 2020): 107653. http://dx.doi.org/10.1016/j.bej.2020.107653.

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20

Lovato, G., C. Z. Lazaro, M. Zaiat, S. M. Ratusznei, and J. A. D. Rodrigues. "Biohydrogen production by co-digesting whey and glycerin in an AnSBBR: Performance optimization, metabolic pathway kinetic modeling and phylogenetic characterization." Biochemical Engineering Journal 128 (December 2017): 93–105. http://dx.doi.org/10.1016/j.bej.2017.09.011.

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21

Glasauer, S., S. Langley, and T. J. Beveridge. "Sorption of Fe (Hydr)Oxides to the Surface of Shewanella putrefaciens: Cell-Bound Fine-Grained Minerals Are Not Always Formed De Novo." Applied and Environmental Microbiology 67, no. 12 (2001): 5544–50. http://dx.doi.org/10.1128/aem.67.12.5544-5550.2001.

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ABSTRACT Shewanella putrefaciens, a gram-negative, facultative anaerobe, is active in the cycling of iron through its interaction with Fe (hydr)oxides in natural environments. Fine-grained Fe precipitates that are attached to the outer membranes of many gram-negative bacteria have most often been attributed to precipitation and growth of the mineral at the cell surface. Our study of the sorption of nonbiogenic Fe (hydr)oxides revealed, however, that large quantities of nanometer-scale ferrihydrite (hydrous ferric oxide), goethite (α-FeOOH), and hematite (α-Fe2O3) adhered to the cell surface. A
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22

Pfeiffer, Peter, and Ferdinand Radler. "Hochleistungsfl�ssigchromatographische Bestimmung von organischen S�uren, Zuckern, Glycerin und Alkohol im Wein an einer Kationenaustauschers�ule." Zeitschrift f�r Lebensmittel-Untersuchung und -Forschung 181, no. 1 (1985): 24–27. http://dx.doi.org/10.1007/bf01124802.

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23

Uchimoto, Takeaki, Yasunori Iwao, Kana Takahashi, et al. "A comparative study of glycerin fatty acid ester and magnesium stearate on the dissolution of acetaminophen tablets using the analysis of available surface area." European Journal of Pharmaceutics and Biopharmaceutics 78, no. 3 (2011): 492–98. http://dx.doi.org/10.1016/j.ejpb.2011.01.014.

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24

Fujikawa, T., S. Imamura, M. Tokumaru, et al. "43 Cryopreservation of Bovine Sperm Using Antifreeze Polyamino-Acid." Reproduction, Fertility and Development 30, no. 1 (2018): 161. http://dx.doi.org/10.1071/rdv30n1ab43.

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Carboxylated poly-l-lysine (CPLL), an ampholytic polymer compound, is reported to have a cryoprotective property similar to that of antifreeze proteins. We previously reported the effectiveness of CPLL as cryoprotective material for bovine sperm (43rd Annual Conference of International Embryo Technology Society, Austin, TX, USA; http://www.iets.org/2017/IETS_2017_Program_Book_FINAL.pdf). In this research, we investigated additional aspects of CPLL for bovine sperm. The conventional cryopreservation medium used for Control group consisted of 6.5% (v/v) glycerin, and the cryopreservation medium
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25

de Albuquerque, Juliane Nogueira, Ana Paula Paulinetti, Giovanna Lovato, Roberta Albanez, Suzana Maria Ratusznei, and José Aberto Domingues Rodrigues. "Anaerobic Sequencing Batch Reactors Co-digesting Whey and Glycerin as a Possible Solution for Small and Mid-size Dairy Industries: Environmental Compliance and Methane Production." Applied Biochemistry and Biotechnology 192, no. 3 (2020): 979–98. http://dx.doi.org/10.1007/s12010-020-03372-0.

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26

Imran, Muhammad, Muhammad Irfan, Muhammad Yaseen, and Naser Rasheed. "Application of Glycerin and Polymer Coated Diammonium Phosphate in Alkaline Calcareous Soil for Improving Wheat Growth, Grain Yield and Phosphorus Use Efficiency." Journal of Crop Science and Biotechnology 21, no. 5 (2018): 425–34. http://dx.doi.org/10.1007/s12892-018-0126-0.

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27

Gao, Haidi, Jia-Hui Liu, Victoria Arantza León Anchustegui, Yulin Chang, Jichuan Zhang, and Yiyang Dong. "The Protective Effects of Graphene Oxide Against the Stress from Organic Solvent by Covering Hela Cells." Current Nanoscience 15, no. 4 (2019): 412–19. http://dx.doi.org/10.2174/1573413714666180821112731.

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Background: In recent years, new nanomaterials have received great attention due to their widespread use in agriculture, food safety and pharmacy. Among them, graphene and graphene oxide (GO) are emerging as promising nanomaterials, which may have far-reaching effects on pharmacy and health. </P><P> Objective: In this paper, the living Hela cells were covered by GO (Hela@GO) and the cell viability, reactive oxygen species, membrane integrity and apoptosis of them were compared with the control Hela cells, especially under the stress from four kinds of organic solvent, including dim
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28

de Oliveira, Marcelo Firmino, Andressa Tironi Vieira, Antônio Carlos Ferreira Batista, Hugo de Souza Rodrigues, and Nelson Ramos Stradiotto. "A Simple and Fast Method for the Production and Characterization of Methylic and Ethylic Biodiesels from Tucum Oil via an Alkaline Route." Journal of Biomedicine and Biotechnology 2011 (2011): 1–4. http://dx.doi.org/10.1155/2011/238474.

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A simple, fast, and complete route for the production of methylic and ethylic biodiesel from tucum oil is described. Aliquots of the oil obtained directly from pressed tucum (pulp and almonds) were treated with potassium methoxide or ethoxide at 40°C for 40 min. The biodiesel form was removed from the reactor and washed with 0.1 M HCl aqueous solution. A simple distillation at 100°C was carried out in order to remove water and alcohol species from the biodiesel. The oxidative stability index was obtained for the tucum oil as well as the methylic and ethylic biodiesel at 6.13, 2.90, and 2.80 h,
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29

Habe, Hiroshi, Tokuma Fukuoka, Dai Kitamoto, and Keiji Sakaki. "Biotransformation of glycerol to d-glyceric acid by Acetobacter tropicalis." Applied Microbiology and Biotechnology 81, no. 6 (2009): 1033–39. http://dx.doi.org/10.1007/s00253-008-1737-2.

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30

HABE, Hiroshi, Yuko SHIMADA, Tokuma FUKUOKA, et al. "Production of Glyceric Acid byGluconobactersp. NBRC3259 Using Raw Glycerol." Bioscience, Biotechnology, and Biochemistry 73, no. 8 (2009): 1799–805. http://dx.doi.org/10.1271/bbb.90163.

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31

Kumar, Ashish, Mamta Belwal, Varun Mohan, Radha Raman Maurya, and Venkataraman Vishwanathan. "Catalytic Vapor Phase Oxidation of Glycerol to Glyceric Acid Over Activated Carbon Supported Gold Nanocatalysts." International Journal of Nanoscience 19, no. 06 (2020): 2050007. http://dx.doi.org/10.1142/s0219581x20500076.

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A series of activated carbon (AC) supported Au nanocatalysts with different loadings of Au were prepared by using the homogeneous deposition–precipitation (HDP) method. The samples were characterised with myriad techniques such as X-ray diffraction (XRD), CO-chemisorption, N2 adsorption–desorption measurements, transmission electron microscopy (TEM), inductively coupled plasma-optical emission spectrometer (ICP-OES) and X-ray photoelectron spectroscopy (XPS) to understand the structural and textural properties in detail. The catalysts were tested for the vapour phase oxidation of glycerol to g
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32

Habe, Hiroshi, Yuko Shimada, Toshiharu Yakushi, et al. "Microbial Production of Glyceric Acid, an Organic Acid That Can Be Mass Produced from Glycerol." Applied and Environmental Microbiology 75, no. 24 (2009): 7760–66. http://dx.doi.org/10.1128/aem.01535-09.

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ABSTRACT Glyceric acid (GA), an unfamiliar biotechnological product, is currently produced as a small by-product of dihydroxyacetone production from glycerol by Gluconobacter oxydans. We developed a method for the efficient biotechnological production of GA as a target compound for new surplus glycerol applications in the biodiesel and oleochemical industries. We investigated the ability of 162 acetic acid bacterial strains to produce GA from glycerol and found that the patterns of productivity and enantiomeric GA compositions obtained from several strains differed significantly. The growth pa
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33

Benito, Santiago. "Combined Use of Lachancea thermotolerans and Schizosaccharomyces pombe in Winemaking: A Review." Microorganisms 8, no. 5 (2020): 655. http://dx.doi.org/10.3390/microorganisms8050655.

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The combined use of Lachancea thermotolerans and Schizosaccharomyces pombe is a new winemaking biotechnology that aims to solve some modern industrial oenology problems related to warm viticulture regions. These areas are characterized for producing musts with high levels of sugar that can potentially be converted into wines with elevated ethanol contents, which are usually associated with high pH levels. This biotechnology was reported for the first time in 2015, and since then, several scientific articles have been published regarding this topic. These reported scientific studies follow an e
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34

Mitchell, Daniel E., Alice E. R. Fayter, Robert C. Deller, Muhammad Hasan, Jose Gutierrez-Marcos, and Matthew I. Gibson. "Ice-recrystallization inhibiting polymers protect proteins against freeze-stress and enable glycerol-free cryostorage." Materials Horizons 6, no. 2 (2019): 364–68. http://dx.doi.org/10.1039/c8mh00727f.

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35

HABE, Hiroshi, Yuko SHIMADA, Tokuma FUKUOKA, et al. "Use of aGluconobacter frateuriiMutant to Prevent Dihydroxyacetone Accumulation during Glyceric Acid Production from Glycerol." Bioscience, Biotechnology, and Biochemistry 74, no. 11 (2010): 2330–32. http://dx.doi.org/10.1271/bbb.100406.

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36

Poblete‐Castro, Ignacio, Christoph Wittmann, and Pablo I. Nikel. "Biochemistry, genetics and biotechnology of glycerol utilization in Pseudomonas species." Microbial Biotechnology 13, no. 1 (2020): 32–53. http://dx.doi.org/10.1111/1751-7915.13400.

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37

Schuch, Ricardo, and Kumar D. Mukherjee. "Radiochemical Methods for Studying Lipase-Catalyzed Interesterification of Lipids." Zeitschrift für Naturforschung C 42, no. 11-12 (1987): 1285–90. http://dx.doi.org/10.1515/znc-1987-11-1223.

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Reactions involving lipase-catalyzed interesterification of lipids, which are of commendable interest in biotechnology, have been monitored and assayed by radiochemical methods using 14C-labeled substrates. Medium chain (C12 plus Cu) triacylglycerols were reacted in the presence of an immobilized lipase from Mucor miehei and hexane at 45 °C with methyl [1-14C]oleate. [1-14C]oleic acid, [carboxyl-14C]trioleoylglycerol. [1-14C)octadecenyl alcohol, and [U-14C)glycerol, each of known specific activity. The reactions were monitored and the rate of interesterification determined by radio thin layer
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38

Heinrich, Daniel, Björn Andreessen, Mohamed H. Madkour, Mansour A. Al-Ghamdi, Ibrahim I. Shabbaj, and Alexander Steinbüchel. "From Waste to Plastic: Synthesis of Poly(3-Hydroxypropionate) in Shimwellia blattae." Applied and Environmental Microbiology 79, no. 12 (2013): 3582–89. http://dx.doi.org/10.1128/aem.00161-13.

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ABSTRACTIn recent years, glycerol has become an attractive carbon source for microbial processes, as it accumulates massively as a by-product of biodiesel production, also resulting in a decline of its price. A potential use of glycerol in biotechnology is the synthesis of poly(3-hydroxypropionate) [poly(3HP)], a biopolymer with promising properties which is not synthesized by any known wild-type organism. In this study, the genes for 1,3-propanediol dehydrogenase (dhaT) and aldehyde dehydrogenase (aldD) ofPseudomonas putidaKT2442, propionate-coenzyme A (propionate-CoA) transferase (pct) ofClo
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39

Poladyan, A. A. "ETHANOL SUPPLEMENTATION AS A NEW APPROACH TO REGULATE GROWTH AND HYDROGEN PRODUCTION OF $ \textit{ESCHERICHIA COLI} $ UPON GLYCEROL FERMENTATION." Proceedings of the YSU B: Chemical and Biological Sciences 54, no. 2 (252) (2020): 138–46. http://dx.doi.org/10.46991/pysu:b/2020.54.2.138.

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Molecular hydrogen (H2) and ethanol are the main by-products of glycerol fermentation by Escherichia coli. In this study, the growth of E. coli BW25113 was investigated with the addition of small amounts (0.05 to 2 %) of ethanol alone and in a combination with glycerol The bacterial growth, the kinetic of the redox potential, and the H2 production in peptone medium, pH 7.5, were investigated upon various amounts of ethanol supplementation. In the presence of any amount of ethanol, but upon the absence of other sources of carbon, no H2 production was observed. Whereas ethanol (0.3 to 1 %) with
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40

Villanova, Valeria, Antonio Emidio Fortunato, Dipali Singh, et al. "Investigating mixotrophic metabolism in the model diatom Phaeodactylum tricornutum." Philosophical Transactions of the Royal Society B: Biological Sciences 372, no. 1728 (2017): 20160404. http://dx.doi.org/10.1098/rstb.2016.0404.

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Diatoms are prominent marine microalgae, interesting not only from an ecological point of view, but also for their possible use in biotechnology applications. They can be cultivated in phototrophic conditions, using sunlight as the sole energy source. Some diatoms, however, can also grow in a mixotrophic mode, wherein both light and external reduced carbon contribute to biomass accumulation. In this study, we investigated the consequences of mixotrophy on the growth and metabolism of the pennate diatom Phaeodactylum tricornutum , using glycerol as the source of reduced carbon. Transcriptomics,
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41

Mooranian, Armin, Corina Mihaela Ionescu, Susbin Raj Wagle, et al. "Chenodeoxycholic Acid Pharmacology in Biotechnology and Transplantable Pharmaceutical Applications for Tissue Delivery: An Acute Preclinical Study." Cells 10, no. 9 (2021): 2437. http://dx.doi.org/10.3390/cells10092437.

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Introduction. Primary bile acids (PBAs) are produced and released into human gut as a result of cholesterol catabolism in the liver. A predominant PBA is chenodeoxycholic acid (CDCA), which in a recent study in our laboratory, showed significant excipient-stabilizing effects on microcapsules carrying insulinoma β-cells, in vitro, resulting in improved cell functions and insulin release, in the hyperglycemic state. Hence, this study aimed to investigate the applications of CDCA in bio-encapsulation and transplantation of primary healthy viable islets, preclinically, in type 1 diabetes. Methods.
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42

Medina, Víctor Guadalupe, Marinka J. H. Almering, Antonius J. A. van Maris, and Jack T. Pronk. "Elimination of Glycerol Production in Anaerobic Cultures of a Saccharomyces cerevisiae Strain Engineered To Use Acetic Acid as an Electron Acceptor." Applied and Environmental Microbiology 76, no. 1 (2009): 190–95. http://dx.doi.org/10.1128/aem.01772-09.

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ABSTRACT In anaerobic cultures of wild-type Saccharomyces cerevisiae, glycerol production is essential to reoxidize NADH produced in biosynthetic processes. Consequently, glycerol is a major by-product during anaerobic production of ethanol by S. cerevisiae, the single largest fermentation process in industrial biotechnology. The present study investigates the possibility of completely eliminating glycerol production by engineering S. cerevisiae such that it can reoxidize NADH by the reduction of acetic acid to ethanol via NADH-dependent reactions. Acetic acid is available at significant amoun
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43

Janek, Tomasz, Eduardo J. Gudiña, Xymena Połomska, et al. "Sustainable Surfactin Production by Bacillus subtilis Using Crude Glycerol from Different Wastes." Molecules 26, no. 12 (2021): 3488. http://dx.doi.org/10.3390/molecules26123488.

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Most biosurfactants are obtained using costly culture media and purification processes, which limits their wider industrial use. Sustainability of their production processes can be achieved, in part, by using cheap substrates found among agricultural and food wastes or byproducts. In the present study, crude glycerol, a raw material obtained from several industrial processes, was evaluated as a potential low-cost carbon source to reduce the costs of surfactin production by Bacillus subtilis #309. The culture medium containing soap-derived waste glycerol led to the best surfactin production, re
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44

Sato, Shun, Naoki Morita, Dai Kitamoto, Toshiharu Yakushi, Kazunobu Matsushita, and Hiroshi Habe. "Change in product selectivity during the production of glyceric acid from glycerol by Gluconobacter strains in the presence of methanol." AMB Express 3, no. 1 (2013): 20. http://dx.doi.org/10.1186/2191-0855-3-20.

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45

Remize, F., L. Barnavon, and S. Dequin. "Glycerol Export and Glycerol-3-phosphate Dehydrogenase, but Not Glycerol Phosphatase, Are Rate Limiting for Glycerol Production in Saccharomyces cerevisiae." Metabolic Engineering 3, no. 4 (2001): 301–12. http://dx.doi.org/10.1006/mben.2001.0197.

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46

Sun, Shangde, Fei Qin, Yanlan Bi, Jingnan Chen, Guolong Yang, and Wei Liu. "Enhanced transesterification of ethyl ferulate with glycerol for preparing glyceryl diferulate using a lipase in ionic liquids as reaction medium." Biotechnology Letters 35, no. 9 (2013): 1449–54. http://dx.doi.org/10.1007/s10529-013-1222-6.

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Heistinger, Lina, Brigitte Gasser, and Diethard Mattanovich. "Microbe Profile: Komagataella phaffii: a methanol devouring biotech yeast formerly known as Pichia pastoris." Microbiology 166, no. 7 (2020): 614–16. http://dx.doi.org/10.1099/mic.0.000958.

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Methylotrophic yeasts of the genus Komagataella are abundantly found in tree exudates. Their ability to utilize methanol as carbon and energy source relies on an assimilation pathway localized in largely expanded peroxisomes, and a cytosolic methanol dissimilation pathway. Other substrates like glucose or glycerol are readily utilized as well. Komagataella yeasts usually grow as haploid cells and are secondary homothallic as they can switch mating type. Upon mating diploid cells sporulate readily, forming asci with four haploid spores. Their ability to secrete high amounts of heterologous prot
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Zheng, Yu, Li Zhao, Jianguo Zhang, Haiyi Zhang, Xingyuan Ma, and Dongzhi Wei. "Production of glycerol from glucose by coexpressing glycerol-3-phosphate dehydrogenase and glycerol-3-phosphatase in Klebsiella pneumoniae." Journal of Bioscience and Bioengineering 105, no. 5 (2008): 508–12. http://dx.doi.org/10.1263/jbb.105.508.

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49

Rittmann, Doris, Steffen N. Lindner, and Volker F. Wendisch. "Engineering of a Glycerol Utilization Pathway for Amino Acid Production by Corynebacterium glutamicum." Applied and Environmental Microbiology 74, no. 20 (2008): 6216–22. http://dx.doi.org/10.1128/aem.00963-08.

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ABSTRACT The amino acid-producing organism Corynebacterium glutamicum cannot utilize glycerol, a stoichiometric by-product of biodiesel production. By heterologous expression of Escherichia coli glycerol utilization genes, C. glutamicum was engineered to grow on glycerol. While expression of the E. coli genes for glycerol kinase (glpK) and glycerol 3-phosphate dehydrogenase (glpD) was sufficient for growth on glycerol as the sole carbon and energy source, additional expression of the aquaglyceroporin gene glpF from E. coli increased growth rate and biomass formation. Glutamate production from
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50

Wang, Zhengxiang, Jian Zhuge, Huiying Fang, and Bernard A. Prior. "Glycerol production by microbial fermentation." Biotechnology Advances 19, no. 3 (2001): 201–23. http://dx.doi.org/10.1016/s0734-9750(01)00060-x.

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