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Artículos de revistas sobre el tema ""grapevine transformation""

Autor: Grafiati

Publicado: 11 de marzo de 2023

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1

Baribault, T. J., K. G. M. Skene, and N. Steele Scott. "Genetic transformation of grapevine cells." Plant Cell Reports 8, no. 3 (1989): 137–40. http://dx.doi.org/10.1007/bf00716825.

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2

Li, Z. T., S. Dhekney, M. Dutt, et al. "Optimizing Agrobacterium-mediated transformation of grapevine." In Vitro Cellular & Developmental Biology - Plant 42, no. 3 (2006): 220–27. http://dx.doi.org/10.1079/ivp2006770.

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3

Dutt, Manjul, Dennis J. Gray, Zhijian T. Li, Sadanand Dhekney, and Marilyn M. Van Aman. "Micropropagation Cultures for Genetic Transformation of Grapevine." HortScience 41, no. 4 (2006): 972C—972. http://dx.doi.org/10.21273/hortsci.41.4.972c.

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A major drawback to the use of embryogenic cultures for transformation of grapevine is that their ability to undergo genetic transformation is cultivar-dependent. Also, depending on cultivar, embryogenic cultures are difficult to impossible to maintain over time, reducing their utility for use in genetic transformation. An alternative to the use of embryogenic cultures for transformation of grapevine is the use of micropropagation cultures, which are easier to initiate from a wide range of grapevine cultivars and can be maintained over time without loss of function. Vitis vinifera `Thompson Se

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4

Cutanda, M. C., P. Chatelet, A. Bouquet, et al. "GENETIC TRANSFORMATION OF 'MACABEO' AND 'TEMPRANILLO' GRAPEVINE CULTIVARS." Acta Horticulturae, no. 827 (May 2009): 641–45. http://dx.doi.org/10.17660/actahortic.2009.827.113.

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5

KOVALENKO, P., and A. GALKIN. "Transformation of Grapevine caber net sauvignon by agrobacterium." Cell Biology International Reports 14 (September 1990): 189. http://dx.doi.org/10.1016/0309-1651(90)90855-s.

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6

Kikkert, J. R., J. R. Vidal, and B. I. Reisch. "APPLICATION OF THE BIOLISTIC METHOD FOR GRAPEVINE GENETIC TRANSFORMATION." Acta Horticulturae, no. 689 (August 2005): 459–62. http://dx.doi.org/10.17660/actahortic.2005.689.54.

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7

Guellec, Véronique, Chantal David, Michel Branchard, and Jacques Tempé. "Agrobacterium rhizogenes mediated transformation of grapevine (Vitis vinifera L.)." Plant Cell Tissue and Organ Culture (PCTOC) 20, no. 3 (1990): 211–15. http://dx.doi.org/10.1007/bf00041883.

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8

Vidal, Jose R., Julie R. Kikkert, Bruno D. Donzelli, Patricia G. Wallace, and Bruce I. Reisch. "Biolistic transformation of grapevine using minimal gene cassette technology." Plant Cell Reports 25, no. 8 (2006): 807–14. http://dx.doi.org/10.1007/s00299-006-0132-7.

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9

Verdugo-Vásquez, Nicolás, Gastón Gutiérrez-Gamboa, Emilio Villalobos-Soublett, and Andrés Zurita-Silva. "Effects of Rootstocks on Blade Nutritional Content of Two Minority Grapevine Varieties Cultivated under Hyper-Arid Conditions in Northern Chile." Agronomy 11, no. 2 (2021): 327. http://dx.doi.org/10.3390/agronomy11020327.

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In the 90s, as in other countries, transformation of Chilean viticulture brought about the introduction and spread of European grapevine varieties which has resulted in a massive loss of minor local and autochthonous grapevine varieties traditionally grown in several wine growing regions. Fortunately, in recent years, autochthonous and minority varieties have been revalued due to their high tolerance to pests and diseases and because of their adaptation to thermal and water stress triggered by global warming. In this study, we assessed the nutritional status of two autochthonous grapevines gra

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10

Deák, Tamás, Tünde Kupi, Róbert Oláh, et al. "Candidate plant gene homologues in grapevine involved in Agrobacterium transformation." Open Life Sciences 8, no. 10 (2013): 1001–9. http://dx.doi.org/10.2478/s11535-013-0218-5.

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AbstractThe grapevine (Vitis vinifera) genome was analyzed in silico for homologues of plant genes involved in Agrobacterium transformation in Arabidopsis thaliana and Nicotiana spp. Grapevine homologues of the glucomannan 4-betamannosyltransferase 9 gene CslA-09 involved in bacterial attachment to the cell wall, homologues of reticulon-like proteins BTI1, 2, 3 and RAB8 GTPases, both involved in T-DNA transfer to the host cell, homologues of VirE2 interacting protein VIP1 that contributes to the targeting of T-DNA into the nucleus and to its integration, and homologues of the histone protein H

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11

Butiuc-Keul, Anca, and Ana Coste. "Biotechnologies and Strategies for Grapevine Improvement." Horticulturae 9, no. 1 (2023): 62. http://dx.doi.org/10.3390/horticulturae9010062.

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Grapevine (Vitis vinifera subsp. Vinifera) is one of the most widespread and economically important perennial fruit crops in the world. Viticulture has changed over the years in response to changing environmental conditions and market demands, triggering the development of new and improved varieties to ensure the crop’s sustainability. The aim of this review is to provide a perspective on the recent developments in biotechnology and molecular biology and to establish the potential of these technologies for the genetic improvement of grapevine. The following aspects are discussed: (i) the impor

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12

ZHANG, Xiu-ming, Yi-fei WU, Zhi LI, Chang-bing SONG, and Xi-ping WANG. "Advancements in plant regeneration and genetic transformation of grapevine (Vitis spp.)." Journal of Integrative Agriculture 20, no. 6 (2021): 1407–34. http://dx.doi.org/10.1016/s2095-3119(20)63586-9.

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13

Repka, V., and I. Baumgartnerová. "Grapevine habituation: Understanding of factors that contribute to neoplastic transformation and somaclonal variation." Acta Agronomica Hungarica 56, no. 4 (2008): 399–408. http://dx.doi.org/10.1556/aagr.56.2008.4.4.

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Two-dimensional gel electrophoresis coupled to protein microarray analysis was used to examine for the first time the molecular mechanisms of grapevine habituation ( Vitis vinifera L., cv. Limberger) at both the proteome and the interactome level. The examination of 2-D maps derived from control and habituated cell cultures revealed the presence of 55 protein spots displaying a differential expression pattern. Using computational prediction methods, fundamental differences were found between eukaryotic interactomes. It was confirmed that all the predicted protein family interactomes (the full

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14

Dutt, Manjul, Zhijian T. Li, Sadanand Dhekney, and Dennis J. Gray. "(285) Characterization of a Composite Promoter from Genomic Sequences of Grapevine." HortScience 41, no. 4 (2006): 1053C—1053. http://dx.doi.org/10.21273/hortsci.41.4.1053c.

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Genetic transformation of plants necessitates the use of promoters to control transgene expression. Numerous promoters have been isolated from a wide range of organisms for use in plants. However, many of these natural promoters exhibit relatively low activity and/or have limited use. To provide an alternative, we constructed a composite promoter (EP) using a genomic DNA sequence and a 35 bp TATA-containing fragment from the 2S albumin (VvAlb1) gene core promoter of grapevine. The 0.9-kb genomic sequence was identified after TAIL-PCR, based on the presence of several unique cis-acting elements

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15

Habili, Nuredin, and Forrest W. Nutter. "Temporal and Spatial Analysis of Grapevine Leafroll-Associated Virus 3 in Pinot Noir Grapevines in Australia." Plant Disease 81, no. 6 (1997): 625–28. http://dx.doi.org/10.1094/pdis.1997.81.6.625.

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An epidemic of grapevine leafroll disease (GLD), caused by grapevine leafroll-associated virus 3 (GLRaV-3), was monitored over an 11-year period in Nuriootpa, South Australia. Inoculum originated from infected budwood, and initial GLD incidence at the time of transplanting in 1986 was 23.1%. Infected vines were planted in a random spatial pattern. Change in disease incidence was not observed until 8 years after planting, when disease incidence increased to 27.9%. Disease incidence increased to 51.9% by 1996. Disease progress and rate curves (dy/dt versus time) indicated that the logistic (R2 =

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16

Li, Zhijian T., S. A. Dhekney, M. Dutt, and D. J. Gray. "An improved protocol for Agrobacterium-mediated transformation of grapevine (Vitis vinifera L.)." Plant Cell, Tissue and Organ Culture 93, no. 3 (2008): 311–21. http://dx.doi.org/10.1007/s11240-008-9378-9.

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17

Martinelli, L., and G. Mandolino. "Genetic transformation and regeneration of transgenic plants in grapevine (Vitis rupestris S.)." Theoretical and Applied Genetics 88, no. 6-7 (1994): 621–28. http://dx.doi.org/10.1007/bf01253963.

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18

Noronha, Henrique, Angélica Silva, Namiki Mitani-Ueno, et al. "The grapevine NIP2;1 aquaporin is a silicon channel." Journal of Experimental Botany 71, no. 21 (2020): 6789–98. http://dx.doi.org/10.1093/jxb/eraa294.

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Abstract Silicon (Si) supplementation has been shown to improve plant tolerance to different stresses, and its accumulation in the aerial organs is mediated by NIP2;1 aquaporins (Lsi channels) and Lsi2-type exporters in roots. In the present study, we tested the hypothesis that grapevine expresses a functional NIP2;1 that accounts for root Si uptake and, eventually, Si accumulation in leaves. Own-rooted grapevine cuttings of the cultivar Vinhão accumulated &gt;0.2% Si (DW) in leaves when irrigated with 1.5 mM Si for 1 month, while Si was undetected in control leaves. Real-time PCR showed t

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19

Nakano, M., Y. Hoshino, and M. Mii. "Regeneration of transgenic plants of grapevine (Vitis viniferaL.) viaAgrobacteriumrhizogenesmediated transformation of embryogenic calli." Journal of Experimental Botany 45, no. 5 (1994): 649–56. http://dx.doi.org/10.1093/jxb/45.5.649.

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20

Sabbadini, S., L. Capriotti, C. Limera, O. Navacchi, G. Tempesta, and B. Mezzetti. "A plant regeneration platform to apply new breeding techniques for improving disease resistance in grapevine rootstocks and cultivars." BIO Web of Conferences 12 (2019): 01019. http://dx.doi.org/10.1051/bioconf/20191201019.

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Worldwide grapevine cultivation is based on the use of elite cultivars, in many cases strictly linked to local important wine brands. Most of Vitis viniferacultivars have high susceptibility to fungal and viral diseases therefore, new breeding techniques (e.g. Cisgenesis, RNAi and gene editing) offer the possibility to introduce new clones of the main cultivars with increased diseases resistance, in order to reduce environmental impact and improve quality in the intensive wine grape industry. This study is finalized to develop efficient in vitro regeneration and transformation protocols to ext

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21

Le Gall, O., L. Torregrosa, Y. Danglot, T. Candresse, and A. Bouquet. "Agrobacterium-mediated genetic transformation of grapevine somatic embryos and regeneration of transgenic plants expressing the coat protein of grapevine chrome mosaic nepovirus (GCMV)." Plant Science 102, no. 2 (1994): 161–70. http://dx.doi.org/10.1016/0168-9452(94)90034-5.

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22

Jardak, Rahma, Ahmed Mliki, Abdelwahed Ghorbel, and Götz M. Reustle. "Transient expression of uidA gene in grapevine protoplasts after PEG-mediated transformation." OENO One 36, no. 2 (2002): 93. http://dx.doi.org/10.20870/oeno-one.2002.36.2.975.

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<p style="text-align: justify;">Leaf protoplasts, isolated from <em>Vitis vinifera</em> «Sakasly» and «Muscat d’Alexandrie» and protoplasts from embryogenic tissue of <em>Vitis</em> sp. «Seyval blanc» were incubated in the presence of PEG in a transformation solution containing the plasmid pBI426 which carries the β-glucuronidase (<em>gus</em>) and the neomycin phosphotransferase II (<em>npt</em>II) genes. The treated protoplasts were cultivated in CPW13 medium without kanamycin selection. 48h after the PEG transformation, transient express

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23

Gray, D. J., Z. T. Li, D. L. Hopkins, et al. "Transgenic Grapevines Resistant to Pierce's Disease." HortScience 40, no. 4 (2005): 1104D—1105. http://dx.doi.org/10.21273/hortsci.40.4.1104d.

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Pierce's disease (PD), caused by the xylem-limited bacterium Xylella fastidiosa, is endemic to the coastal plain of the southeastern United States. Although native southern grapevines are tolerant to X. fastidiosa, all varieties of Vitisvinifera grown in the region will succumb to PD. Genetic transformation to add disease resistance genes, while not disturbing desirable phenotypic characters, holds promise for expanding the southeastern U.S. grape industry by allowing use of established fruit and wine varieties. We utilize embryogenic cell cultures and Agrobacterium strain EHA105 to refine tra

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24

Kikkert, Julie R., Dominique Hébert-Soulé, Patricia G. Wallace, Michael J. Striem, and Bruce I. Reisch. "Transgenic plantlets of ‘Chancellor’ grapevine (Vitis sp.) from biolistic transformation of embryogenic cell suspensions." Plant Cell Reports 15, no. 5 (1996): 311–16. http://dx.doi.org/10.1007/bf00232362.

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25

Jardak-Jamoussi, Rahma, Patrick Winterhagen, Badra Bouamama, et al. "Development and evaluation of a GFLV inverted repeat construct for genetic transformation of grapevine." Plant Cell, Tissue and Organ Culture (PCTOC) 97, no. 2 (2009): 187–96. http://dx.doi.org/10.1007/s11240-009-9514-1.

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26

Kikkert, Julie R., Dominique H�bert-Soul�, Patricia G. Wallace, Michael J. Striem, and Bruce I. Reisch. "Transgenic plantlets of 'Chancellor' grapevine ( Vitis sp.) from biolistic transformation of embryogenic cell suspensions." Plant Cell Reports 15, no. 5 (1996): 311–16. http://dx.doi.org/10.1007/s002990050023.

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27

Shin, Hye Young, Gi Hoon Kim, Sang Jae Kang, Jeung-Sul Han, and Cheol Choi. "Optimization of Agrobacterium-mediated transformation procedure for grapevine ‘Kyoho’ with carrot antifreeze protein gene." Journal of Plant Biotechnology 44, no. 4 (2017): 388–93. http://dx.doi.org/10.5010/jpb.2017.44.4.388.

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28

Müller, Carsten, Kristina Ullmann, and Pablo Steinberg. "The Grapevine-Shoot Extract Vineatrol30 Inhibits the Chemically Induced Malignant Transformation of BALB/c-3T3 Cells." Journal of Medicinal Food 14, no. 1-2 (2011): 34–39. http://dx.doi.org/10.1089/jmf.2010.0022.

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29

Sanjurjo, Laura, José Ramón Vidal, Antonio Segura, and Francisco de la Torre. "Genetic transformation of grapevine cells using the minimal cassette technology: The need of 3′-end protection." Journal of Biotechnology 163, no. 4 (2013): 386–90. http://dx.doi.org/10.1016/j.jbiotec.2012.11.014.

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30

Creasap, J. E., C. L. Reid, M. C. Goffinet, R. Aloni, C. Ullrich, and T. J. Burr. "Effect of Wound Position, Auxin, and Agrobacterium vitis Strain F2/5 on Wound Healing and Crown Gall in Grapevine." Phytopathology® 95, no. 4 (2005): 362–67. http://dx.doi.org/10.1094/phyto-95-0362.

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Agrobacterium vitis is the causal agent of crown gall disease in grapevine, which can be severe in many regions worldwide. Vitis vinifera cultivars are highly susceptible to freeze injury, providing the wounds necessary for infection by A. vitis. Wound position in relation to the uppermost bud of cuttings was determined to be important in tumor development. Inoculated wounds below buds developed tumors, whereas wounds opposite the bud did not, implying that indole-3-aectic acid flow contributes to tumor formation. If auxin was applied to wounds prior to inoculation with a tumorigenic A. vitis

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31

Gribaudo, Ivana, Valentina Scariot, Giorgio Gambino, Andrea Schubert, Richard Göller, and Margit Laimer. "TRANSFORMATION OF VITIS VINIFERA L. CV NEBBIOLO WITH THE COAT PROTEIN GENE OF GRAPEVINE FANLEAF VIRUS (GFLV)." Acta Horticulturae, no. 603 (April 2003): 309–14. http://dx.doi.org/10.17660/actahortic.2003.603.40.

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32

Martinelli, L., D. Costa, V. Poletti, et al. "GENETIC TRANSFORMATION OF TOBACCO AND GRAPEVINE FOR RESISTANCE TO VIRUSES RELATED TO THE RUGOSE WOOD DISEASE COMPLEX." Acta Horticulturae, no. 528 (May 2000): 323–30. http://dx.doi.org/10.17660/actahortic.2000.528.44.

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33

Khan, Nadeem, Fizza Fatima, Muhammad Salman Haider, et al. "Genome-Wide Identification and Expression Profiling of the Polygalacturonase (PG) and Pectin Methylesterase (PME) Genes in Grapevine (Vitis vinifera L.)." International Journal of Molecular Sciences 20, no. 13 (2019): 3180. http://dx.doi.org/10.3390/ijms20133180.

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In pectin regulation, polygalacturonases (PGs) and pectin methylesterases (PMEs) are critical components in the transformation, disassembly network, and remodeling of plant primary cell walls. In the current study, we identified 36 PG and 47 PME genes using the available genomic resources of grapevine. Herein, we provide a comprehensive overview of PGs and PMEs, including phylogenetic and collinearity relationships, motif and gene structure compositions, gene duplications, principal component analysis, and expression profiling during developmental stages. Phylogenetic analysis of PGs and PMEs

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34

Landi, Lucia, Sergio Murolo, and Gianfranco Romanazzi. "Colonization of Vitis spp. Wood by sGFP-Transformed Phaeomoniella chlamydospora, a Tracheomycotic Fungus Involved in Esca Disease." Phytopathology® 102, no. 3 (2012): 290–97. http://dx.doi.org/10.1094/phyto-06-11-0165.

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To evaluate wood colonization and interactions with Vitis spp. of Phaeomoniella chlamydospora, a fungal agent involved in Esca disease, isolate CBS 229.95 was transformed using a pCT74 construct which contained the genetic markers for synthetic green fluorescent protein (sGFP) and hygromycin B phosphotransferase. Nine stable P. chlamydospora fungal transformants (Pch-sGFP lines) were obtained using polyethylene-glycol-mediated transformation of protoplasts. These were characterized for sgfp and hygromycin B phosphotransferase (hph) genome insertions and for sGFP fluorescence emission, using qu

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35

Felber, Aretusa Cristina, Julio Cesar Polonio, Ravely Casarotti Orlandelli, et al. "Agrobacterium-Mediated Transformation of Diaporthe schini Endophytes Associated with Vitis labrusca L. and Its Antagonistic Activity Against Grapevine Phytopathogens." Indian Journal of Microbiology 59, no. 2 (2019): 217–24. http://dx.doi.org/10.1007/s12088-019-00787-0.

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36

Aleynova, Olga A., Andrey R. Suprun, Alexey A. Ananev, et al. "Effect of Calmodulin-like Gene (CML) Overexpression on Stilbene Biosynthesis in Cell Cultures of Vitis amurensis Rupr." Plants 11, no. 2 (2022): 171. http://dx.doi.org/10.3390/plants11020171.

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Stilbenes are plant phenolics known to rapidly accumulate in grapevine and other plants in response to injury or pathogen attack and to exhibit a great variety of healing beneficial effects. It has previously been shown that several calmodulin-like protein (CML) genes were highly up-regulated in cell cultures of wild-growing grapevine Vitis amurensis Rupr. in response to stilbene-modulating conditions, such as stress hormones, UV-C, and stilbene precursors. Both CML functions and stilbene biosynthesis regulation are still poorly understood. In this study, we investigated the effect of overexpr

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37

López-Pérez, A. J., J. Carreño, and M. Dabauza. "TRANSFORMATION OF EMBRYOGENIC CALLUS AND TRANSGENIC PLANT REGENERATION IN TABLE GRAPEVINE 'SUGRAONE' (VITIS VINIFERA L.): EFFECT OF AGROBACTERIUM TUMEFACIENS STRAIN." Acta Horticulturae, no. 827 (May 2009): 415–20. http://dx.doi.org/10.17660/actahortic.2009.827.71.

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38

Fan, Chaohong, Ni Pu, Xiping Wang, et al. "Agrobacterium-mediated genetic transformation of grapevine (Vitis vinifera L.) with a novel stilbene synthase gene from Chinese wild Vitis pseudoreticulata." Plant Cell, Tissue and Organ Culture 92, no. 2 (2007): 197–206. http://dx.doi.org/10.1007/s11240-007-9324-2.

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39

Bouamama, Badra, Asma Ben Salem-Fnayou, Hatem Ben Jouira, Abdelwahed Ghorbel, and Ahmed Mliki. "Influence of the flower stage and culture medium on the induction of somatic embryogenesis from anther culture in Tunisian grapevine cultivars." OENO One 41, no. 4 (2007): 185. http://dx.doi.org/10.20870/oeno-one.2007.41.4.835.

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<p style="text-align: justify;"><strong>Aims</strong>: The aim of this research is to identify the optimal flower developmental stage, in order to establish an efficient regeneration protocol via somatic embryogenesis in several local grapevine (Vitis vinifera L.) cultivars, using anther culture.</p><p style="text-align: justify;"><strong>Methods and results</strong>: Immature anthers sampled on fruity cuttings, at three flower developmental stages were cultured on three media [MS (1962), NN (1969) and CP (1987)] to which several phytohormonal combinat

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40

Monteiro, Patrı́cia B., Diva C. Teixeira, Renê R. Palma, Monique Garnier, Joseph-Marie Bové, and Joël Renaudin. "Stable Transformation of the Xylella fastidiosa Citrus Variegated Chlorosis Strain withoriC Plasmids." Applied and Environmental Microbiology 67, no. 5 (2001): 2263–69. http://dx.doi.org/10.1128/aem.67.5.2263-2269.2001.

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ABSTRACT Xylella fastidiosa is a gram-negative, xylem-limited bacterium affecting economically important crops (e.g., grapevine, citrus, and coffee). The citrus variegated chlorosis (CVC) strain ofX. fastidiosa is the causal agent of this severe disease of citrus in Brazil and represents the first plant-pathogenic bacterium for which the genome sequence was determined. Plasmids for the CVC strain of X. fastidiosa were constructed by combining the chromosomal replication origin (oriC) of X. fastidiosa with a gene which confers resistance to kanamycin (Kanr). In plasmid p16KdAori, the oriCfragme

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41

Martínez-Márquez, Ascensión, Jaime Morante-Carriel, Karla Ramírez-Estrada, et al. "A reliable protocol for the stable transformation of non-embryogenic cells cultures of grapevine (Vitis vinifera L.) and Taxus x media." Journal of Biological Methods 2, no. 2 (2015): 21. http://dx.doi.org/10.14440/jbm.2015.51.

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42

Lizamore, Darrell, and Chris Winefield. "The addition of an organosilicone surfactant to Agrobacterium suspensions enables efficient transient transformation of in vitro grapevine leaf tissue at ambient pressure." Plant Cell, Tissue and Organ Culture (PCTOC) 120, no. 2 (2014): 607–15. http://dx.doi.org/10.1007/s11240-014-0627-9.

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43

Esterio, M., J. Auger, C. Ramos, and H. García. "First Report of Fenhexamid Resistant Isolates of Botrytis cinerea on Grapevine in Chile." Plant Disease 91, no. 6 (2007): 768. http://dx.doi.org/10.1094/pdis-91-6-0768c.

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Botrytis cinerea Pers. (teleomorph Botryotinia fuckeliana (de Bary) Whetzel) is a haploid, filamentous ascomycete that causes gray mold on many economically important crops in temperate regions, especially grapevine. The management of gray mold on table grape in Chile involves cultural and chemical methods. Currently, protection programs are based on several fungicide families (dicarboximides, anilinopyrimidines, mixture of anilinopyrimidines and phenylpyrroles, and hydroxyanilides [fenhexamid]). During the last 25 years, B. cinerea developed resistance to virtually all specific fungicides use

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44

Szankowski, I., K. Briviba, J. Fleschhut, J. Sch�nherr, H.-J. Jacobsen, and H. Kiesecker. "Transformation of apple ( Malus domestica Borkh.) with the stilbene synthase gene from grapevine ( Vitis vinifera L.) and a PGIP gene from kiwi ( Actinidia deliciosa )." Plant Cell Reports 22, no. 2 (2003): 141–49. http://dx.doi.org/10.1007/s00299-003-0668-8.

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45

Chen, Qiuju, Bohan Deng, Jie Gao, et al. "Comparative Analysis of miRNA Abundance Revealed the Function of Vvi-miR828 in Fruit Coloring in Root Restriction Cultivation Grapevine (Vitis vinifera L.)." International Journal of Molecular Sciences 20, no. 16 (2019): 4058. http://dx.doi.org/10.3390/ijms20164058.

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Root restriction cultivation leads to early maturation and quality improvement, especially in the anthocyanin content in grapevine. However, the molecular mechanisms that underlie these changes have not been thoroughly elucidated. In this study, four small RNA libraries were constructed, which included the green soft stage (GS) and ripe stage (RS) of ‘Muscat’ (Vitis vinifera L.) grape berries that were grown under root restriction (RR) and in traditional cultivation (no root restriction, CK). A total of 162 known miRNAs and 14 putative novel miRNAs were detected from the four small RNA librari

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Zhang, Xiaowei, Fangxiu Xue, Zepeng Wang, et al. "A Novel Method of Hyperbola Recognition in Ground Penetrating Radar (GPR) B-Scan Image for Tree Roots Detection." Forests 12, no. 8 (2021): 1019. http://dx.doi.org/10.3390/f12081019.

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Ground penetrating radar (GPR), as a newly nondestructive testing technology (NDT), has been adopted to explore the spatial position and the structure of the tree roots. Due to the complexity of soil distribution and the randomness of the root position in the natural environment, it is difficult to locate the root in the GPR B-Scan image. In this study, a novel method for root detection in the B-scan image by considering both multidirectional features and symmetry of hyperbola was proposed. Firstly, a mixed dataset B-Scan images were employed to train Faster RCNN (Regions with CNN features) to

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Sedlo, Jiří, and Pavel Tomšík. "Strategic development of varietal vineyards in the Czech Republic." Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis 60, no. 2 (2012): 325–34. http://dx.doi.org/10.11118/actaun201260020325.

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The paper describes strategic changes in the structure of grapevine (Vitis vinifera L.) varieties grown in the Czech Republic. In 2004–2005, (i.e. after the admission of the Czech Republic into the EU) expenditures associated with restructuralisation and transformation of vineyards amounted for CZK 25,423 thous. The authors examine the development taking place in this domain within the last 50 years (i.e. from 1960 to 2010) and pay detailed attention to the period of 1989 to 2010. The paper analyses reasons of these changes and tries to describe the future development expected after 2010. The

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Biasi, Rita, Roberta Farina, and Elena Brunori. "Family Farming Plays an Essential Role in Preserving Soil Functionality: A Study on Active Managed and Abandoned Traditional Tree Crop-Based Systems." Sustainability 13, no. 7 (2021): 3967. http://dx.doi.org/10.3390/su13073967.

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In traditional agricultural areas, where traditional crops (TCs) are cultivated, small farms are still highly represented. Located prevalently in marginal and sensitive areas, agricultural areas have undergone deep transformation. Smallholders have maintained the traditional asset of cultivation (extensive and low input requirement management) only to some extent. In some cases they have adapted traditional orchards into more intensive planting systems. Frequently, they have abandoned agriculture. The land use and management influence soil functions, i.e., the capability of a specific soil to

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Bai, Yunhe, Xiaowen Zhang, Xuxian Xuan, et al. "miR3633a-GA3ox2 Module Conducts Grape Seed-Embryo Abortion in Response to Gibberellin." International Journal of Molecular Sciences 23, no. 15 (2022): 8767. http://dx.doi.org/10.3390/ijms23158767.

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Seedlessness is one of the important quality and economic traits favored by grapevine consumers, which are mainly affected by phytohormones, especially gibberellin (GA). GA is widely utilized in seedless berry production and could effectively induce grape seed embryo abortion. However, the molecular mechanism underlying this process, like the role of RNA silencing in the biosynthesis pathway of GA remains elusive. Here, Gibberellin 3-β dioxygenase2 (GA3ox2) as the last key enzyme in GA biosynthesis was predicated as a potential target gene for miR3633a, and two of them were identified as a GA

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Scorza, R., J. M. Cordts, D. J. Gray, D. W. Ramming, and R. L. Emershad. "Transformation of Grape (Vitis vinifera L.)." HortScience 30, no. 4 (1995): 876F—876. http://dx.doi.org/10.21273/hortsci.30.4.876f.

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Transgenic grapevines were regenerated from somatic embryos produced from immature zygotic embryos of two seedless grape selections and from leaves of in vitro-grown plants of `Thompson Seedless'. Somatic embryos were bombarded with gold microparticles using the Biolistic PDS-1000/He device (Bio-Rad Labs) and then exposed to engineered A. tumefaciens EHA101 (E. Hood, WSU). Alternately, somatic embryos were exposed to A. tumefaciens without bombardment. Following cocultivation, secondary embryos multiplied on Emershad and Ramming proliferation medium under kan selection. Transgenic embryos were

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