Bibliografías temáticas / Integrity-PCR

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Literatura académica sobre el tema "Integrity-PCR"

Autor: Grafiati
Publicado: 9 de noviembre de 2024
Última modificación: 31 de julio de 2025

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Artículos de revistas sobre el tema "Integrity-PCR"

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Perkin, L. C., B. Oppert, S. Duke, and C. P.-C. Suh. "Assessment of DNA Integrity From Trap-Captured Boll Weevil (Coleoptera: Curculionidae) for Use in a New PCR-Based Diagnostic Tool." Journal of Economic Entomology 114, no. 3 (2021): 1321–28. http://dx.doi.org/10.1093/jee/toab073.

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Abstract The boll weevil, Anthonomus grandis grandis Boheman (Coleoptera: Curculionidae), is a major pest of commercial cotton (Gossypium hirsutum) in the southern United States and throughout Central and South America. Efforts are underway to develop a PCR-based diagnostic tool that can be used to rapidly and accurately differentiate boll weevils from other weevil species that are commonly captured in pheromone traps. However, the quantity and integrity of weevil DNA must be sufficient for a successful PCR assay. Currently, active eradication programs service traps weekly, but post-eradicatio
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Bergerová, E., Z. Godálová, and P. Siekel. "Combined effects of temperature, pressure and low pH on the amplification of DNA of plant derived foods." Czech Journal of Food Sciences 29, No. 4 (2011): 337–45. http://dx.doi.org/10.17221/217/2010-cjfs.

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The effect of food processing on the DNA integrity was studied by means of PCR amplification of soybean, transgenic MON 810 and non-transgenic maize, bean, and pea. The degree of DNA degradation was checked by PCR and visualised by agarose gel electrophoresis. The conditions of technological treatment such as temperature, pH, pressure, and their combination may negatively influence the integrity of DNA in processed foods and hence PCR detection of food components. The DNA over 300 bp was amplifiable when mild processing parameters up to 100°C were performed at approximately neutral or
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Du, Xiongwei, Hongjie An, Bo Jin, Liangyu Meng, and Qingdai Liu. "Carbon Nanotubes Altering Specificity of Repeated PCR and DNA Integrity Properties." Journal of Nanoscience and Nanotechnology 14, no. 7 (2014): 5547–51. http://dx.doi.org/10.1166/jnn.2014.8874.

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Frasquilho, Sonia G., Ignacio Sanchez, Changyoung Yoo, Laurent Antunes, Camille Bellora, and William Mathieson. "Do Tissues Fixed in a Non-crosslinking Fixative Require a Dedicated Formalin-free Processor?" Journal of Histochemistry & Cytochemistry 69, no. 6 (2021): 389–405. http://dx.doi.org/10.1369/00221554211017859.

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We evaluate the consequences of processing alcohol-fixed tissue in a processor previously used for formalin-fixed tissue. Biospecimens fixed in PAXgene Tissue Fixative were cut into three pieces then processed in a flushed tissue processor previously used for formalin-fixed, paraffin-embedded (FFPE) blocks (neutral buffered formalin [NBF]+ve), a formalin-free system (NBF−ve), or left unprocessed. Histomorphology and immunohistochemistry were compared using hematoxylin/eosin staining and antibodies for MLH-1, Ki-67, and CK-7. Nucleic acid was extracted using the PAXgene Tissue RNA/DNA kits and
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Raja, Siva, Jesus Ching, Liqiang Xi, et al. "Technology for Automated, Rapid, and Quantitative PCR or Reverse Transcription-PCR Clinical Testing." Clinical Chemistry 51, no. 5 (2005): 882–90. http://dx.doi.org/10.1373/clinchem.2004.046474.

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Abstract Background: PCR-based assays can improve clinical care, but they remain technically demanding and labor-intensive. We describe a new instrument, the GeneXpert®, that performs automated nucleic acid isolation, reverse transcription, and fluorescence-based quantitative PCR in ∼35 min. Methods: Yield and integrity of RNA isolated on the GeneXpert were compared with Qiagen-based extraction for parallel samples (5-μm frozen tissue sections). The reproducibility of automated RNA isolation, reverse transcription, and quantitative PCR was determined by replicate (n = 10) analysis of 10 tissue
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Fleige, Simone, and Michael W. Pfaffl. "RNA integrity and the effect on the real-time qRT-PCR performance." Molecular Aspects of Medicine 27, no. 2-3 (2006): 126–39. http://dx.doi.org/10.1016/j.mam.2005.12.003.

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Gorzelak-Pabis, Paulina, Emilia Luczak, Katarzyna Wojdan, et al. "Endothelial integrity may be regulated by a specific antigen via an IgE-mediated mechanism." Postępy Higieny i Medycyny Doświadczalnej 71, no. 1 (2017): 0. http://dx.doi.org/10.5604/01.3001.0010.3800.

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Background: Human vascular endothelial function and integrity may be regulated by many non-specific factors. However, the potential influence of specific antigens via an IgE-mediated mechanism remains unknown. The aim of the study was to determine the expression of the IgE receptors FcεRI and FcεRII in the human vascular endothelium and to assess their relevance in the IgE-mediated regulation of endothelial integrity.Material/Methods: FcεRI and FcεRII expression in human umbilical vein endothelial cells (HUVEC) was genetically assessed by PCR with respective primers and sequencing. HUVEC were
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Oakey, H. Jane. "A Universal Test to Determine the Integrity of RNA, and its Suitability for Reverse Transcription, in Animal Tissue Laboratory Specimens." Journal of Veterinary Diagnostic Investigation 19, no. 5 (2007): 459–64. http://dx.doi.org/10.1177/104063870701900501.

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Degradation of RNA in diagnostic specimens can cause false-negative test results and potential misdiagnosis when tests rely on the detection of specific RNA sequence. Current molecular methods of checking RNA integrity tend to be host species or group specific, necessitating libraries of primers and reaction conditions. The objective here was to develop a universal (multi-species) quality assurance tool for determining the integrity of RNA in animal tissues submitted to a laboratory for analyses. Ribosomal RNA (16S rRNA) transcribed from the mitochondrial 16S rDNA was used as template material
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Cédile, Oriane, Sólja Remisdóttir Veyhe, Marcus Høy Hansen, Kjell Titlestad, and Charlotte Guldborg Nyvold. "Investigation of circulating DNA integrity after blood collection." BioTechniques 71, no. 5 (2021): 550–55. http://dx.doi.org/10.2144/btn-2020-0167.

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Method summary Concentrations of circulating DNA in blood plasma were compared using NanoDrop, Qubit, quantitative PCR and Bioanalyzer, and DNA integrity was evaluated with the Bioanalyzer according to the time of plasma preparation.
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Didelot, Audrey, Steve K. Kotsopoulos, Audrey Lupo, et al. "Multiplex Picoliter-Droplet Digital PCR for Quantitative Assessment of DNA Integrity in Clinical Samples." Clinical Chemistry 59, no. 5 (2013): 815–23. http://dx.doi.org/10.1373/clinchem.2012.193409.

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BACKGROUND Assessment of DNA integrity and quantity remains a bottleneck for high-throughput molecular genotyping technologies, including next-generation sequencing. In particular, DNA extracted from paraffin-embedded tissues, a major potential source of tumor DNA, varies widely in quality, leading to unpredictable sequencing data. We describe a picoliter droplet–based digital PCR method that enables simultaneous detection of DNA integrity and the quantity of amplifiable DNA. METHODS Using a multiplex assay, we detected 4 different target lengths (78, 159, 197, and 550 bp). Assays were validat
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Tesis sobre el tema "Integrity-PCR"

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Garlan, Fanny. "Nouvelles méthodes de détection de l'ADN tumoral circulant par PCR digitale en gouttelettes : application au suivi des patients." Thesis, Sorbonne Paris Cité, 2016. http://www.theses.fr/2016USPCB108/document.

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L’ADN tumoral circulant (ADNtc) porte des altérations spécifiques de la tumeur des patients, qui sont détectables par un acte minimalement invasif. L’ADNtc représente donc un biomarqueur d’intérêt pour le suivi de l’évolution du cancer. Sa détection requière une technique hautement sensible et quantitative. Dans ce contexte, ce travail de thèse a porté sur la quantification et le suivi de l’ADNtc par PCR digitale en gouttelettes (PCRdg). Cet outil permet la détection d’altérations à l’échelle d’un ADN unique, offrant ainsi une sensibilité allant jusqu’à 0.001%. La détection de cet ADNtc a été
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Normand, Camille. "La rhinοpneumοnie équine : caractérisatiοn mοléculaire et cellulaire de l'herpèsvirus équin 4, un mοdèle d'étude pοur l'appοrt de cοnnaissances dans la pathοgénie de la maladie, la survie et l'intégrité du virus ainsi que l'identificatiοn de mοlécules antivirales". Electronic Thesis or Diss., Normandie, 2024. http://www.theses.fr/2024NORMC405.

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Jusqu’en 1981, l’herpèsvirus équin 4 (HVE-4) et l’HVE-1, responsables de la rhinopneumonie, étaient considérés comme deux sous-types d’un même virus. Il a depuis été démontré que l’HVE-4 était très peu impliqué dans les avortements et son implication dans la forme nerveuse de la maladie n’est pas démontrée à ce jour. C’est probablement la raison qui fait que ce virus est moins étudié que l’HVE-1. Ce travail de thèse a porté sur trois aspects importants de la rhinopneumonie chez le cheval afin d’apporter de la connaissance sur l’HVE-4 et d’évaluer l’intérêt d’utiliser ce virus comme modèle. Nou
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Capítulos de libros sobre el tema "Integrity-PCR"

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Pinz, Sophia, Eva Doskocil, and Wolfgang Seufert. "Thermofluor-Based Analysis of Protein Integrity and Ligand Interactions." In Ribosome Biogenesis. Springer US, 2022. http://dx.doi.org/10.1007/978-1-0716-2501-9_15.

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AbstractThermofluor is a fluorescence-based thermal shift assay, which measures temperature-induced protein unfolding and thereby yields valuable information about the integrity of a purified recombinant protein. Analysis of ligand binding to a protein is another popular application of this assay. Thermofluor requires neither protein labeling nor highly specialized equipment, and can be performed in a regular real-time PCR instrument. Thus, for a typical molecular biology laboratory, Thermofluor is a convenient method for the routine assessment of protein quality. Here, we provide Thermofluor
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"Trusted Cloud Computing." In Detection and Mitigation of Insider Attacks in a Cloud Infrastructure. IGI Global, 2019. http://dx.doi.org/10.4018/978-1-5225-7924-3.ch002.

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This chapter introduces various ideas to deal with insider attacks using the research directions, which are discussed in earlier chapters such as remote attestation, sealed storage, and integrity measurement. Trusted computing dependent on hardware root of trust has been produced by industry to secure computing frameworks and billions of end points. Remote attestation provides a facility to attestation the required platforms using platform configuration registers (PCR), and sealed storage is used to encrypt the consumer sensitive data using cryptographic operations. Integrity measurements are
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Embleton, M. Jim. "Reverse transcriptase in situ PCR for RNA detection." In PCR3. Oxford University PressOxford, 1997. http://dx.doi.org/10.1093/oso/9780199636327.003.0005.

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Abstract Most published applications of in situ PCR for amplification of specific nucleic acid sequences in cells or tissues have been for the detection of genes or viruses which are present in low copy numbers or in a minority of the cell population. Detection may be either indirect, for example by hybridizing a labelled oligonucleotide probe to the amplified DNA, or direct, where the product is amplified with labelled oligonucleotide primers. A variety of labels for oligonucleotides have been used in different situations, including fluorescent dyes for direct microscopic visualization (1,2),
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Krzyściak, Wirginia, Marta Szwajca, Paulina Karcz, et al. "Myo-inositol’s Role in Understanding the Pain Perception in Patients with Schizophrenia." In New Approaches to the Management and Diagnosis of Schizophrenia [Working Title]. IntechOpen, 2024. http://dx.doi.org/10.5772/intechopen.1005244.

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The molecular explanation for the changes in pain perception in schizophrenia lies in nerve inflammation. The decrease in inositol, mainly localized in glial cells, can support these changes. There are also significant alterations in the viability and functioning of neurons, which are linked to a significant reduction of N-acetyl-aspartate (NAA). Our study demonstrates significantly increased myo-inositol levels in the anterior and posterior cingulate cortex. An increase in the myo-inositol/sum of the creatinine and phosphocreatinine (Cr + PCr) ratio and NAA levels additionally supports the no
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Panwar, Gautam, Ankur Vashishtha, Navdeep Singh, and Salender Singh. "BACTERIAL LABORATORY DIAGNOSIS OF CLINICAL SAMPLES." In COMPENDIUM OF MEDICAL DIAGNOSTIC TECHNOLOGY. NOBLE SCIENCE PRESS, 2023. http://dx.doi.org/10.52458/9789388996846.nsp2023.eb.ch-07.

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Bacterial infections encompass a diverse array of diseases, each with unique clinical presentations and implications. Accurate and timely diagnosis of these infections is essential for effective patient management, infection control, and antimicrobial stewardship. This chapter provides a comprehensive exploration of the intricate process of bacterial laboratory diagnosis, highlighting critical steps and methodologies. Starting with the collection and transportation of clinical samples, we underscore the significance of maintaining sample integrity to minimize contamination risks. Precise sampl
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Actas de conferencias sobre el tema "Integrity-PCR"

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Manna, Kathleen, Joseph Moore, Kenneth Wunch, et al. "Relative Performance of Various 16S iTag Amplicon Sequencing Primer Pairs in Profiling Microbial Communities Relevant to Oil & Gas Operations." In CORROSION 2019. NACE International, 2019. https://doi.org/10.5006/c2019-13442.

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Abstract The oil and gas industry's efforts to characterize microbial communities in oilfield process fluids has shed increasing light on the influence of bacteria and archaea on hydrocarbon production. Notably, topside or downhole water contamination by microorganisms such as sulfate-reducing bacteria, acid-producing bacteria, and/or other halophiles can negatively impact asset integrity and reduce the quality and quantity of produced hydrocarbons. Molecular microbiology methods have contributed to our understanding of these complex processes by providing unprecedented resolution of resident
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Gao, Y., J. Wang, D. Willberg, C. Lai, and S. Makarychev-Mikhailov. "Leveraging Machine Learning and X-Ray Fluorescence Spectrometry for Elemental Analysis of Pozzolanic Materials." In SPE International Conference on Oilfield Chemistry. SPE, 2025. https://doi.org/10.2118/224272-ms.

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Abstract Pozzolanic materials, known for their superior performance in enhancing the strength and integrity of well cements along with their significantly reduced carbon footprint, have been receiving much attention. The elemental characterization of pozzolanic materials is crucial because it affects the reactivity of cementitious fluids and thus the overall efficiency of a cementing job. This study investigates the elemental analysis correlation between inductively coupled plasma optical emission spectroscopy (ICP-OES) and X-ray fluorescence (XRF) spectrometry techniques using machine learnin
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Informes sobre el tema "Integrity-PCR"

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Qamhia, Issam, Erol Tutumluer, and Han Wang. Aggregate Subgrade Improvements Using Quarry By-products: A Field Investigation. Illinois Center for Transportation, 2021. http://dx.doi.org/10.36501/0197-9191/21-017.

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This report presents a case study for constructing aggregate subgrade improvement (ASI) layers using quarry by-product aggregates (QBA), a quarry mix of large primary crushed rocks (PCR) and sand-sized quarry fines. The construction took place at Larry Power Road in Bourbonnais Township in Kankakee County, Illinois, where the Illinois Department of Transportation placed two QBA mixes. The first mix (QBA_M1) consisted of 45% quarry by-products and 55% railroad ballast–sized 3×1 PCR. The second mix (QBA_M2) consisted of 31% and 69% quarry by-products and PCR, respectively. Two conventional ASI s
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Cahaner, Avigdor, Sacit F. Bilgili, Orna Halevy, Roger J. Lien, and Kellye S. Joiner. effects of enhanced hypertrophy, reduced oxygen supply and heat load on breast meat yield and quality in broilers. United States Department of Agriculture, 2014. http://dx.doi.org/10.32747/2014.7699855.bard.

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Original objectivesThe objectives of this project were to evaluate the growth performance, meat yield and quality attributes of broiler strains widely differing in their genetic potential under normal temperature vs. warm temperature (short and long-term) conditions. Strain differences in breast muscle accretion rate, metabolic responses under heat load and, gross and histopathological changes in breast muscle under thermal load was also to be characterized. BackgroundTremendous genetic progress has been made in broiler chicken growth rate and meat yield since the 1950s. Higher growth rate is
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