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Artículos de revistas sobre el tema "Isolation methods"

Autor: Grafiati

Publicado: 4 de junio de 2021

Última modificación: 20 de febrero de 2023

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1

Moťková, P., and J. Vytřasová. "Comparison of methods for isolating fungal DNA." Czech Journal of Food Sciences 29, Special Issue (January 4, 2012): S76—S85. http://dx.doi.org/10.17221/266/2011-cjfs.

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In this study methods of fungal DNA isolation were optimised and compared. The aim of the isolation processes was to obtain DNA of sufficient quality and quantity necessary for its amplification, as most detection techniques require DNA amplification before the proper DNA detection itself. For this purpose, classic methods of DNA extraction were compared and optimised while isolations using commercial kits were also done. The methods were evaluated from several perspectives, with focus especially laid on the isolated DNA not contain PCR inhibitors which would prevent DNA amplification, thus in

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2

Pariset, Eloise, Vincent Agache, and Arnaud Millet. "Extracellular Vesicles: Isolation Methods." Advanced Biosystems 1, no. 5 (April 24, 2017): 1700040. http://dx.doi.org/10.1002/adbi.201700040.

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3

Gilmore, Paul, and Umesh Gandhi. "Development of disc spring stack containment methods for vibration isolation." INTER-NOISE and NOISE-CON Congress and Conference Proceedings 263, no. 2 (August 1, 2021): 4871–79. http://dx.doi.org/10.3397/in-2021-2865.

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Cone disc springs exhibit quasi-zero stiffness behavior that is useful in isolating objects from low frequency vibrations. However, the stroke of a single disc spring is too low for most applications, and springs are stacked to increase the displacement. A method to contain the isolator stack then becomes critical for practical uses. Many challenges in developing these containment methods have been identified and can be collectively described as how to appropriately contain the stack without affecting isolation performance. In this work, three designs are considered: a retaining ring design, t

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4

Kallergi, Galatea, Eleni Politaki, Saad Alkahtani, Christos Stournaras, and Vassilis Georgoulias. "Evaluation of Isolation Methods for Circulating Tumor Cells (CTCs)." Cellular Physiology and Biochemistry 40, no. 3-4 (2016): 411–19. http://dx.doi.org/10.1159/000452556.

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Background: Detection of CTCs is a poor prognostic factor for many cancer types; however, their very low frequency represents an obstacle for their detection. The objective of the current study was to compare the performance of commonly used methods for CTCs isolation. Methods: The evaluated methods using spiking experiments of MCF7, SKBR3 and MDA MB-231 breast cancer cell lines were (i) ficoll density gradient separation (DGS), (ii) red blood cell lysis (Erythrolysis) isolation, (iii) positive immunomagnetic selection (EpCAM Dynal beads), (iv) two different negative immunomagnetic separation

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5

Hosek, J., P. Svastova, M. Moravkova, I. Pavlik, and M. Bartos. "Methods of mycobacterial DNA isolation from different biological material: a review." Veterinární Medicína 51, No. 5 (March 20, 2012): 180–92. http://dx.doi.org/10.17221/5538-vetmed.

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Mycobacteria cause serious infections in animals and human beings. Huge economic losses on farms are caused by selected species of this wide family. A high risk of transmission of infection from animal to human exists. The knowledge of exact pathogen characteristics is an important factor which can improve quick and adequate healing. Cultivation and determination of phenotype is still the &ldquo;gold standard&rdquo;, but has the disadvantage of taking a long time and also low detection limit. Biochemical characterisation of isolates is not exact, and it is expensive. A more popular met

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6

Sadrtdinova, G. R. "K. OXYTOCA BACTERIOPHAGES ISOLATION METHODS IMPROVEMENT." Russian Journal of Infection and Immunity 7, no. 4 (January 1, 2017): 413–18. http://dx.doi.org/10.15789/2220-7619-2017-4-413-418.

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7

Fox, Gary L. "Methods and Apparatus for Isolation System." Journal of the Acoustical Society of America 130, no. 2 (2011): 1083. http://dx.doi.org/10.1121/1.3625660.

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8

Veksler, Naum, Jean-Louis Izbicki, Jean-Marc Conoir, and Pascal Rembert. "Methods of Isolation of Modal Resonances." Applied Mechanics Reviews 51, no. 7 (July 1, 1998): 449–74. http://dx.doi.org/10.1115/1.3099015.

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The scattering problem by immersed targets involves the resonance phenomenon. Different methods of isolation of modal resonances are discussed: the Resonant Scattering Theory (the different backgrounds used in this method are considered), the phase gradient method (which is partly independent of the background choice), and the Argand diagram method (leading to both theoretical and experimental determination of the frequency and the width of resonances) and an exact description of the resonance components of partial modes (involving the determination of the roots of the characteristic equation

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9

Teti, Anna, Anna Taranta, Ida Villanova, Irene Recchia, and Silvia Migliaccio. "Osteoclast Isolation: New Developments and Methods." Journal of Bone and Mineral Research 14, no. 7 (July 1, 1999): 1251–52. http://dx.doi.org/10.1359/jbmr.1999.14.7.1251.

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10

POWELL, T. "Methods of isolation of cardiac myocytes." Journal of Molecular and Cellular Cardiology 18 (1986): 30. http://dx.doi.org/10.1016/s0022-2828(86)80571-5.

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11

Barnes, Ella M. "Isolation methods for anaerobes in foods." International Journal of Food Microbiology 2, no. 1-2 (June 1985): 81–87. http://dx.doi.org/10.1016/0168-1605(85)90060-1.

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12

Price, E. H., and G. H. Hunt. "Aeromonas in hospital—methods of isolation." Journal of Hospital Infection 8, no. 3 (November 1986): 309–11. http://dx.doi.org/10.1016/0195-6701(86)90131-3.

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13

Lopatin, S. A., G. N. Rudenskaya, I. M. Popova, and T. M. Bikbov. "Chromatographic methods of mackerel proteases isolation." Food / Nahrung 35, no. 5 (1991): 415–20. http://dx.doi.org/10.1002/food.19910350502.

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14

Holson, R. R., A. C. Scallet, S. F. Ali, and B. B. Turner. "“Isolation stress” revisited: Isolation-rearing effects depend on animal care methods." Physiology & Behavior 49, no. 6 (June 1991): 1107–18. http://dx.doi.org/10.1016/0031-9384(91)90338-o.

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15

Cronn, Richard, and Jonathan F. Wendel. "Simple methods for isolating homoeologous loci from allopolyploid genomes." Genome 41, no. 6 (December 1, 1998): 756–62. http://dx.doi.org/10.1139/g98-078.

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During allopolyploid formation, divergent sets of chromosomes are combined in a common nucleus. Accordingly, loci that are single-copy in progenitor diploid genomes become duplicated, homoeologous loci in allopolyploids. Although homoeologous loci have been identified using classical genetic and linkage mapping approaches, there are few examples of the isolation and molecular characterization of homoeologous loci from allopolyploids. Here we describe two methods for the isolation of orthologous duplicated loci and demonstrate the feasibility of the techniques using allotetraploid cotton. The m

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16

Vaid, Rajesh Kumar, Taruna Anand, Priyanka Batra, Ram Avtar Legha, and Bhupendra Nath Tripathi. "Evaluation of DNA Extraction Methods of Mule Dung." Defence Life Science Journal 4, no. 3 (July 15, 2019): 170–74. http://dx.doi.org/10.14429/dlsj.4.14269.

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DNA isolation is a critical step in microbial community analysis of animal dung. DNA isolation from mule dung is challenging due to microbial diversity, composition and chemical nature of mule dung. Therefore, selection of an appropriate DNA isolation method is important to analyse the complete microbial diversity. In the current study, we evaluated the DNA isolation from mule dung samples (n=11) using QiAmp Mini stool kit as per manufacturer’s procedure with modifications. The results suggest that modifications in proprietary column based method improved the DNA quality and quantity suitable

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17

Ruebsamen, Dale T. "Vibration Isolation Apparatus and Methods of Manufacture." Journal of the Acoustical Society of America 131, no. 2 (2012): 1672. http://dx.doi.org/10.1121/1.3685657.

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18

Natonek-Wiśniewska, M., and E. Słota. "Methods of DNA isolation from poultry meal." Journal of Animal and Feed Sciences 16, no. 3 (September 6, 2007): 490–96. http://dx.doi.org/10.22358/jafs/66805/2007.

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19

Ponomarenko, E. A., O. V. Makarova, A. M. Kosyreva, M. V. Kondashevskaya, M. A. Diatroptova, I. S. Tsvetkov, A. A. Stepanov, L. P. Mikhailova, and K. A. Artem’eva. "Comparative characteristics of spermatogenic cells isolation methods." CLINICAL AND EXPERIMENTAL MORPHOLOGY 8, no. 3 (2019): 55–63. http://dx.doi.org/10.31088/cem2019.8.3.55-63.

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20

Chih-Yuan Lee, Tung-Sheng Chen, and Chin-Hsing Kao. "Methods for noise isolation in RFCMOS ICs." IEEE Electron Device Letters 24, no. 7 (July 2003): 478–80. http://dx.doi.org/10.1109/led.2003.815001.

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21

Bracho, Julio C., Arístides Rosado, and Jorge A. Pino. "Comparison of Isolation Methods for Propolis Volatiles." Journal of Essential Oil Research 8, no. 6 (November 1996): 665–68. http://dx.doi.org/10.1080/10412905.1996.9701037.

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22

Jones, Leslie P. "Root isolation methods based upon lagrangian interpolation." International Journal of Computer Mathematics 24, no. 3-4 (January 1988): 343–55. http://dx.doi.org/10.1080/00207168808803652.

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23

Schmidt, Stefan R., Fritz Schweikart, and Martin E. Andersson. "Current methods for phosphoprotein isolation and enrichment." Journal of Chromatography B 849, no. 1-2 (April 2007): 154–62. http://dx.doi.org/10.1016/j.jchromb.2006.09.016.

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24

HANKA, LADISLAV J., and RONDA D. SCHAADT. "Methods for isolation of streptoverticillia from soils." Journal of Antibiotics 41, no. 4 (1988): 576–78. http://dx.doi.org/10.7164/antibiotics.41.576.

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25

Sloboda, Roger D. "Isolation of Microtubules by Assembly/Disassembly Methods." Cold Spring Harbor Protocols 2015, no. 1 (January 2015): pdb.prot081182. http://dx.doi.org/10.1101/pdb.prot081182.

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26

Taylor, Harry Owen, Stephanie Herbers, Samuel Talisman, and Nancy Morrow-Howell. "Assessing Social Isolation: Pilot Testing Different Methods." Journal of Gerontological Social Work 59, no. 3 (April 2, 2016): 228–33. http://dx.doi.org/10.1080/01634372.2016.1197354.

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27

Lozhkin, A. V., and E. I. Sakanyan. "Natural coumarins: Methods of isolation and analysis." Pharmaceutical Chemistry Journal 40, no. 6 (June 2006): 337–46. http://dx.doi.org/10.1007/s11094-006-0123-6.

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28

Williamson, Elizabeth M. "Methods in Biotechnology 4. Natural Products Isolation." Phytotherapy Research 13, no. 5 (August 1999): 451. http://dx.doi.org/10.1002/(sici)1099-1573(199908/09)13:5<451::aid-ptr531>3.0.co;2-p.

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29

Otsuru, S., T. Hofmann, P. Raman, T. Olson, and E. Horwitz. "Equivalent MSC preparations using two isolation methods." Cytotherapy 16, no. 4 (April 2014): S75. http://dx.doi.org/10.1016/j.jcyt.2014.01.276.

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30

Shvydkaya, Maria G., A. M. Zatevalov, S. D. Mitrokhin, and D. T. Dzhandarova. "Comparison of culture and isolation methods for Clostridioides difficile and other anaerobes from stool samples in a routine microbiological laboratory practice." Clinical Microbiology and Antimicrobial Chemotherapy 23, no. 2 (2021): 212–16. http://dx.doi.org/10.36488/cmac.2021.2.212-216.

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Objective. To improve stool sample culture and isolation of anaerobic flora, including Clostridioides difficile in the routine microbiological laboratory practice at the children’s oncology hospital. Materials and Methods. A total of 517 stool samples collected from patients in children’s oncology hospital from 2013 to 2015 were studied. All samples were analyzed by ELISA for C. difficile toxins and by culture according to dedicated 5 schemes for isolation of anaerobic bacteria, including C. difficile. Statistical significance of differences in isolation rates between the studied groups (cultu

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31

Ponomareva, N. I., A. P. Kostyusheva, S. A. Brezgin, V. V. Smirnov, V. I. Gegechkory, D. S. Kostyushev, and V. P. Chulanov. "Comparison of exosome isolation methods for biomedical research." Journal Biomed 17, no. 3E (October 26, 2021): 76–79. http://dx.doi.org/10.33647/2713-0428-17-3e-76-79.

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Exosomes are nanoscale membrane vesicles secreted by almost all kinds of cells and stably existing in the body. Due to their unique properties, exosomes can be used in biomedical research as regenerative preparations or drug delivery vehicles. In this study, an anion exchange (AIEX) chromatography-based protocol for isolation of extracellular vesicles from a cell-conditioned medium was developed. The exosome isolation method based on AIEX chromatography overperforms the canonical ultracentrifugation-based isolation method in terms of its capability of large-volume processing.

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32

Et. al., Cici Jennifer Raj J,. "Influence at Mass of the Base Isolation System in Affecting the Higher Modes of Vibration." Turkish Journal of Computer and Mathematics Education (TURCOMAT) 12, no. 2 (April 10, 2021): 1809–15. http://dx.doi.org/10.17762/turcomat.v12i2.1518.

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Base Isolation is a technology of mitigating the effects due to earthquakes with the aspect of dissipating the seismic waves away from the superstructure, by isolating the superstructure from the ground.This concept is widely essential to be implemented in structures(buildings) irrespective of many factors. There are several materials which could be implemented as base isolator, however the need in reduction of the number of the isolators is essential dueto various factors which a developing country finds difficult to implement. In this paper, a three-storey unsymmetrical building to be consid

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33

Agarwal, Anuj, Ashish Kumar, Bhuvnesh Kumar Singh, Neelanchal Trivedi, and K. K. Jha. "A review on extraction and phytochemical screening methods." Research in Pharmacy and Health Sciences 2, no. 2 (May 15, 2016): 130–37. http://dx.doi.org/10.32463/rphs.2016.v02i02.25.

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Plants are the source of different drugs belonging to various therapeutic categories like antidiabetics, antispasmodics, antihypertensive, anticancer, antidepressants, antimicrobials, etc. Plants are used to treat various ailments and these plants have been used by different individuals and tribals worldwide. Use of plants to treat various ailments have also been mentioned in Ayurveda. Along these lines, various researchers are involved in isolating and assessing different bioactive molecules, to be isolated from various plant sources. Isolation of bioactive molecules is not an easy task for r

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34

Tereza, Sovová, Křížová Barbora, and Ovesná Jaroslava. "Determining the optimal method for DNA isolation from fruit jams." Czech Journal of Food Sciences 36, No. 2 (May 7, 2018): 126–32. http://dx.doi.org/10.17221/340/2017-cjfs.

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DNA extraction is a crucial step in PCR analysis especially when analysing food samples that can be degraded and can potentially contain PCR-inhibiting substances. In this study, we compared the suitability of three DNA extraction methods – two kits: DNeasy<sup>®</sup> Plant Mini Kit and NucleoSpin<sup>®</sup> Food, and the CTAB method – for DNA extraction from commercial fruit jams. Fourteen jams with different contents of fruit, sugar and other additives were extracted in triplicate using the above-mentioned methods directly and after a washing step. The concentration

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35

Dijk, Manon van, t. holt, tte Severin, Suzanne Polinder, and M. Vos. "The Daily Direct Costs of Isolating Patients Identified With Highly Resistant Microorganisms." Infection Control & Hospital Epidemiology 41, S1 (October 2020): s404. http://dx.doi.org/10.1017/ice.2020.1053.

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Background: Isolation precautions are recommended when caring for patients identified with highly resistant microorganisms (HRMOs). However, the direct costs of isolating patients are largely unknown. Therefore, we aimed to obtain detailed information on the daily direct costs associated with isolating patients identified with HRMO. Methods: This study was performed from November until December 2017 on a 12-bed surgical ward. This ward contained solely isolation rooms with an anteroom. The daily direct costs of isolation were based on three cost items: (1) additional personal protective equipm

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36

Janowski, Daniel, Robin Wilgan, Tomasz Leski, Leszek Karliński, and Maria Rudawska. "Effective Molecular Identification of Ectomycorrhizal Fungi: Revisiting DNA Isolation Methods." Forests 10, no. 3 (March 1, 2019): 218. http://dx.doi.org/10.3390/f10030218.

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A better understanding of ectomycorrhizal symbiosis leads to numerous advancements in forest management and environmental protection. The morphological identification of the ectomycorrhizae often proves to be misleading. For this reason, in order to study the ectomycorrhizal fungi communities, a number of molecular methods that require the isolation of nucleic acids are being used. However, ectomycorrhizal root tips, low mass heterogenic material rich in inhibitors, are a recalcitrant substrate in DNA isolation. It is common for published studies to include some number of unidentified root tip

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37

Mercadal, Marina, Carolina Herrero, Olga López-Rodrigo, Manel Castells, Alexandre de la Fuente, Francesc Vigués, Lluís Bassas, and Sara Larriba. "Impact of Extracellular Vesicle Isolation Methods on Downstream miRNA Analysis in Semen: A Comparative Study." International Journal of Molecular Sciences 21, no. 17 (August 19, 2020): 5949. http://dx.doi.org/10.3390/ijms21175949.

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Seminal plasma (SP) contains a unique concentration of miRNA, mostly contained in small extracellular vesicles (sEVs) such as exosomes, some of which could be clinically useful for diagnosis and/or prognosis of urogenital diseases such as prostate cancer (PCa). We optimized several exosome-EV isolation technologies for their use in semen, evaluating EV purifying effectiveness and impact on the downstream analysis of miRNAs against results from the standard ultracentrifugation (UC) method to implement the use of SP sEV_miRNAs as noninvasive biomarkers for PCa. Our results evidenced that commerc

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38

Merga, J. Y., A. J. H. Leatherbarrow, C. Winstanley, M. Bennett, C. A. Hart, W. G. Miller, and N. J. Williams. "Comparison ofArcobacterIsolation Methods, and Diversity ofArcobacterspp. in Cheshire, United Kingdom." Applied and Environmental Microbiology 77, no. 5 (December 30, 2010): 1646–50. http://dx.doi.org/10.1128/aem.01964-10.

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ABSTRACTThe aims of this study were, firstly, to compare five published methods for the isolation ofArcobacterspp. from animal feces in order to determine the most sensitive and specific method. Second, we analyzed the resulting isolates by multilocus sequence typing (MLST) in order to investigate the diversity of the isolates recovered. Third, we investigated the ability to recoverArcobacterspp. from frozen fecal samples. Seventy-seven fecal samples from cattle, sheep, and badgers were subjected to five isolation methods, based on published methods for the isolation ofArcobacterandCampylobact

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39

Vernerova, Andrea, Luiz Felipe Garcia-Souza, Ondrej Soucek, Milan Kostal, Vit Rehacek, Lenka Kujovska Krcmova, Erich Gnaiger, and Ondrej Sobotka. "Mitochondrial Respiration of Platelets: Comparison of Isolation Methods." Biomedicines 9, no. 12 (December 8, 2021): 1859. http://dx.doi.org/10.3390/biomedicines9121859.

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Multiple non-aggregatory functions of human platelets (PLT) are widely acknowledged, yet their functional examination is limited mainly due to a lack of standardized isolation and analytic methods. Platelet apheresis (PA) is an established clinical method for PLT isolation aiming at the treatment of bleeding diathesis in severe thrombocytopenia. On the other hand, density gradient centrifugation (DC) is an isolation method applied in research for the analysis of the mitochondrial metabolic profile of oxidative phosphorylation (OXPHOS) in PLT obtained from small samples of human blood. We studi

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40

Aziz, Mohammad A., Benedict Seo, Haizal M. Hussaini, Merilyn Hibma, and Alison M. Rich. "Comparing Two Methods for the Isolation of Exosomes." Journal of Nucleic Acids 2022 (October 25, 2022): 1–6. http://dx.doi.org/10.1155/2022/8648373.

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Exosomes are membrane-bound nanovesicles released by cells into their extracellular environment. They carry different types of RNA including mRNA which may be useful in the diagnosis of various diseases. Exosome isolation has been a challenge because of their small size; therefore, two exosome isolation methods were compared in this study. The Exoquick-TC PLUS™ exosome isolation kit (kit) was compared with the classic ultracentrifugation (UC) method for exosome isolation. In samples obtained using both methods, cryo-electron microscopy showed round or slightly elongated vesicles with diameters

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41

Bonvin, Debora, Diego Chiappe, Marc Moniatte, Heinrich Hofmann, and Marijana Mionić Ebersold. "Methods of protein corona isolation for magnetic nanoparticles." Analyst 142, no. 20 (2017): 3805–15. http://dx.doi.org/10.1039/c7an00646b.

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42

Stam, Janine, Sabine Bartel, Rainer Bischoff, and Justina C. Wolters. "Isolation of extracellular vesicles with combined enrichment methods." Journal of Chromatography B 1169 (April 2021): 122604. http://dx.doi.org/10.1016/j.jchromb.2021.122604.

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43

KOMATSU, Tsunehiko, and Toshiro NAGASAWA. "Isolation Methods of Megakaryocytes from Human Bone Marrow." Japanese Journal of Thrombosis and Hemostasis 4, no. 1 (1993): 36–39. http://dx.doi.org/10.2491/jjsth.4.36.

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44

Paget, Michelle, Hilary Murray, Clifford J. Bailey, and Richard Downing. "Human islet isolation: semi-automated and manual methods." Diabetes and Vascular Disease Research 4, no. 1 (March 2007): 7–12. http://dx.doi.org/10.3132/dvdr.2007.010.

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45

Shao-jun, Shou, Liu Jing-li, and Guo Xin-sheng. "Methods for testing disturbance isolation of EO system." Journal of Applied Optics 36, no. 4 (2015): 504–8. http://dx.doi.org/10.5768/jao201536.0401002.

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46

Feng, Jie, Yan Li, and Yu Nie. "Methods of mouse cardiomyocyte isolation from postnatal heart." Journal of Molecular and Cellular Cardiology 168 (July 2022): 35–43. http://dx.doi.org/10.1016/j.yjmcc.2022.04.007.

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47

Dundar, Tolga Turan, Mustafa Aziz Hatiboglu, Zehragul Ergul, Mehmet Hakan Seyithanoglu, Elif Sozen, Saffet Tuzgen, Mehmet Yasar Kaynar, and Erdal Karaoz. "Glioblastoma Stem Cells and Comparison of Isolation Methods." Journal of Clinical Medicine Research 11, no. 6 (2019): 415–21. http://dx.doi.org/10.14740/jocmr3781.

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48

BOLTON, F. J. (Eric). "Campylobacter Infections: Food-borne Sources and Isolation Methods." Japanese Journal of Food Microbiology 24, no. 4 (2007): 151–56. http://dx.doi.org/10.5803/jsfm.24.151.

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49

Veksler, Naum, Jean‐Mark Conoir, Jean‐Louis Izbicki, and Pascal Rembert. "Methods of isolation of modal resonances (a review)." Journal of the Acoustical Society of America 99, no. 4 (April 1996): 2599–603. http://dx.doi.org/10.1121/1.415302.

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50

Hayakawa, Masayuki. "Selective isolation methods and distribution of soil actinomycetes." Actinomycetologica 4, no. 2 (1990): 103–12. http://dx.doi.org/10.3209/saj.4_103.

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