Artículos de revistas sobre el tema "Lipid membranes, mechanical properties, atomic force microscopy, scattering"

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1

Domingues, Marco M., Bárbara Gomes, Axel Hollmann, and Nuno C. Santos. "25-Hydroxycholesterol Effect on Membrane Structure and Mechanical Properties." International Journal of Molecular Sciences 22, no. 5 (2021): 2574. http://dx.doi.org/10.3390/ijms22052574.

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Cholesterol is responsible for the plasticity of plasma membranes and is involved in physiological and pathophysiological responses. Cholesterol homeostasis is regulated by oxysterols, such as 25-hydroxycholesterol. The presence of 25-hydroxycholesterol at the membrane level has been shown to interfere with several viruses’ entry into their target cells. We used atomic force microscopy to assess the effect of 25-hydroxycholesterol on different properties of supported lipid bilayers with controlled lipid compositions. In particular, we showed that 25-hydroxycholesterol inhibits the lipid-conden
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2

Cosentino, Katia, Edward Hermann, Nicolai von Kügelgen, Joseph D. Unsay, Uris Ros, and Ana J. García-Sáez. "Force Mapping Study of Actinoporin Effect in Membranes Presenting Phase Domains." Toxins 13, no. 9 (2021): 669. http://dx.doi.org/10.3390/toxins13090669.

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Equinatoxin II (EqtII) and Fragaceatoxin C (FraC) are pore-forming toxins (PFTs) from the actinoporin family that have enhanced membrane affinity in the presence of sphingomyelin (SM) and phase coexistence in the membrane. However, little is known about the effect of these proteins on the nanoscopic properties of membrane domains. Here, we used combined confocal microscopy and force mapping by atomic force microscopy to study the effect of EqtII and FraC on the organization of phase-separated phosphatidylcholine/SM/cholesterol membranes. To this aim, we developed a fast, high-throughput proces
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3

Sadžak, Anja, Janez Mravljak, Nadica Maltar-Strmečki, et al. "The Structural Integrity of the Model Lipid Membrane during Induced Lipid Peroxidation: The Role of Flavonols in the Inhibition of Lipid Peroxidation." Antioxidants 9, no. 5 (2020): 430. http://dx.doi.org/10.3390/antiox9050430.

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The structural integrity, elasticity, and fluidity of lipid membranes are critical for cellular activities such as communication between cells, exocytosis, and endocytosis. Unsaturated lipids, the main components of biological membranes, are particularly susceptible to the oxidative attack of reactive oxygen species. The peroxidation of unsaturated lipids, in our case 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC), induces the structural reorganization of the membrane. We have employed a multi-technique approach to analyze typical properties of lipid bilayers, i.e., roughness, thickness, elas
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4

Efremov, Yuri M., Liubov Shimolina, Alexander Gulin, et al. "Correlation of Plasma Membrane Microviscosity and Cell Stiffness Revealed via Fluorescence-Lifetime Imaging and Atomic Force Microscopy." Cells 12, no. 21 (2023): 2583. http://dx.doi.org/10.3390/cells12212583.

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The biophysical properties of cells described at the level of whole cells or their membranes have many consequences for their biological behavior. However, our understanding of the relationships between mechanical parameters at the level of cell (stiffness, viscoelasticity) and at the level of the plasma membrane (fluidity) remains quite limited, especially in the context of pathologies, such as cancer. Here, we investigated the correlations between cells’ stiffness and viscoelastic parameters, mainly determined via the actin cortex, and plasma membrane microviscosity, mainly determined via it
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5

Basu, Amrita, Pabitra Maity, Prasanta Karmakar, and Sanat Karmakar. "Preparation of Giant Unilamellar Vesicles and Solid Supported Bilayer from Large Unilamellar Vesicles: Model Biological Membranes." Journal of Surface Science and Technology 32, no. 3-4 (2017): 85. http://dx.doi.org/10.18311/jsst/2016/7753.

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Giant Unilamellar Vesicles (GUV) and supported planar membranes are excellent model biological systems for studying the structure and functions of membranes. We have prepared GUV from Large Unilamellar Vesicles (LUV) using electroformation and Supported planar Lipid Bilayer (SLB) by vesicle fusion method. LUV was prepared using an extrusion method and was characterized using Dynamic Light Scattering (DLS) and zeta potential measurements. The techniques for obtaining GUV as well as SLB from LUV have been demonstrated. We have directly observed the formation of GUV under phase contrast microscop
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6

Sumarokova, Maria, Rais Pavlov, Tatiana Lavushchenko, et al. "SARS-CoV-2 FP1 Destabilizes Lipid Membranes and Facilitates Pore Formation." International Journal of Molecular Sciences 26, no. 2 (2025): 686. https://doi.org/10.3390/ijms26020686.

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SARS-CoV-2 viral entry requires membrane fusion, which is facilitated by the fusion peptides within its spike protein. These predominantly hydrophobic peptides insert into target membranes; however, their precise mechanistic role in membrane fusion remains incompletely understood. Here, we investigate how FP1 (SFIEDLLFNKVTLADAGFIK), the N-terminal fusion peptide, modulates membrane stability and barrier function across various model membrane systems. Through a complementary suite of biophysical techniques—including electrophysiology, fluorescence spectroscopy, and atomic force microscopy—we de
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7

Blessing, Bailey, Cory Trout, Abneris Morales, et al. "The Impact of Composition and Morphology on Ionic Conductivity of Silk/Cellulose Bio-Composites Fabricated from Ionic Liquid and Varying Percentages of Coagulation Agents." International Journal of Molecular Sciences 21, no. 13 (2020): 4695. http://dx.doi.org/10.3390/ijms21134695.

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Blended biocomposites created from the electrostatic and hydrophobic interactions between polysaccharides and structural proteins exhibit useful and unique properties. However, engineering these biopolymers into applicable forms is challenging due to the coupling of the material’s physicochemical properties to its morphology, and the undertaking that comes with controlling this. In this particular study, numerous properties of the Bombyx mori silk and microcrystalline cellulose biocomposites blended using ionic liquid and regenerated with various coagulation agents were investigated. Specifica
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8

Lee, Dongjun, Daeun Kim, Seungbin Kim, and Insung Bae. "Cellulose-Based Crosslinked and Reinforced Sulfonated Poly(ether ether ketone) Membranes for Robust Proton Exchange Membranes in Fuel Cells." ECS Meeting Abstracts MA2024-02, no. 43 (2024): 2924. https://doi.org/10.1149/ma2024-02432924mtgabs.

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Proton exchange membrane fuel cells (PEMFCs) are a promising clean energy solution due to their high efficiency and eco-friendly operation. Central to their function is the proton exchange membrane (PEM), typically composed of perfluorosulfonic acid (PFSA) ionomers like Nafion and Aquivion. However, challenges such as cost, gas crossover, and limited temperature range persist. To address these, researchers explore hydrocarbon (HC)-based polymers like sulfonated poly(ether ether ketone) (SPEEK), prized for cost-effectiveness and stability. However, HC-based polymers suffer from issues like unst
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9

Holmbäck, Jan, Vibhu Rinwa, Tobias Halthur, Puneet Rinwa, Anders Carlsson, and Bengt Herslöf. "AKVANO®: A Novel Lipid Formulation System for Topical Drug Delivery—In Vitro Studies." Pharmaceutics 14, no. 4 (2022): 794. http://dx.doi.org/10.3390/pharmaceutics14040794.

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A novel formulation technology called AKVANO® has been developed with the aim to provide a tuneable and versatile drug delivery system for topical administration. The vehicle is based on a water-free lipid formulation where selected lipids, mainly phospholipids rich in phosphatidylcholine, are dissolved in a volatile solvent, such as ethanol. With the aim of describing the basic properties of the system, the following physicochemical methods were used: viscometry, dynamic light scattering, NMR diffusometry, and atomic force microscopy. AKVANO formulations are non-viscous, with virtually no or
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10

Zhou, Jun, Xiaoqing Cao, Linlin Li, Xingcheng Cui, and Yu Fu. "A Novel Strategy for Fabricating a Strong Nanoparticle Monolayer and Its Enhanced Mechanism." Nanomaterials 9, no. 10 (2019): 1468. http://dx.doi.org/10.3390/nano9101468.

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This work presents the preparation of cross-linking Au nanoparticle (NP) monolayer membranes by the thiol exchange reaction and their enhanced mechanical properties. Dithiol molecules were used as a cross-linking mediator to connect the adjacent nanoparticles by replacing the original alkanethiol ligand in the monolayer. After cross-linking, the membrane integrity was maintained and no significant fracture was observed, which is crucial for the membrane serving as a nanodevice. TEM (Transmission Electron Microscopy), UV–Vis absorption spectrum, and GISAXS (grazing incidence small angle X-ray s
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11

Foti, Alice, Luana Calì, Salvatore Petralia, and Cristina Satriano. "Green Nanoformulations of Polyvinylpyrrolidone-Capped Metal Nanoparticles: A Study at the Hybrid Interface with Biomimetic Cell Membranes and In Vitro Cell Models." Nanomaterials 13, no. 10 (2023): 1624. http://dx.doi.org/10.3390/nano13101624.

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Noble metal nanoparticles (NP) with intrinsic antiangiogenic, antibacterial, and anti-inflammatory properties have great potential as potent chemotherapeutics, due to their unique features, including plasmonic properties for application in photothermal therapy, and their capability to slow down the migration/invasion speed of cancer cells and then suppress metastasis. In this work, gold (Au), silver (Ag), and palladium (Pd) NP were synthesized by a green redox chemistry method with the reduction of the metal salt precursor with glucose in the presence of polyvinylpyrrolidone (PVP) as stabilizi
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12

Kirmizitaş, Fatma Ceren, Hanife Sevgi Varli, Murat Demirbilek, and Nelisa Türkoğlu. "Effect of Surfactant on controlling the Size of Octadecylamine-based Solid Lipid Nanoparticles and Toxicity." Research Journal of Biotechnology 18, no. 7 (2023): 131–41. http://dx.doi.org/10.25303/1807rjbt1310141.

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Surfactants play a major role in solid lipid nanoparticles’ (SLNs) composition and properties, especially in terms of particle size and zeta potential. Not only the type of the surfactants but also their amounts and chemical compatibility with the lipid core within the formulation cause good changes. In this study, our goal was to determine the effect of tween 80 (a non-ionic surfactant) on octadecylamine (cationic lipid)-based SLN formulation and its influence on SLNs properties. Also, we examined the effect of sonication and filtration as mechanical forces on the particle size, zeta potentia
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13

Alsadiq, Hussain, and Zahra Alhay. "Mechanical Properties of Medical Microbubbles and Echogenic Liposomes—A Review." Micromachines 16, no. 5 (2025): 588. https://doi.org/10.3390/mi16050588.

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Lipid-shelled microbubbles (MBs) and echogenic liposomes (ELIPs) have been proposed as acoustofluidic theranostic agents after having been proven to be efficient in diagnostics as ultrasonic contrast agents. Their mechanical properties—such as shell stiffness, friction, and resonance frequency—are critical to their performance, stability, oscillatory dynamics, and response to sonication. A precise characterization of these properties is essential for optimizing their biomedical applications, however the current methods vary significantly in their sensitivity and accuracy. This review examines
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14

Retana Moreira, Lissette, Alexa Prescilla-Ledezma, Alberto Cornet-Gomez, et al. "Biophysical and Biochemical Comparison of Extracellular Vesicles Produced by Infective and Non-Infective Stages of Trypanosoma cruzi." International Journal of Molecular Sciences 22, no. 10 (2021): 5183. http://dx.doi.org/10.3390/ijms22105183.

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Extracellular vesicles (EVs) are small lipid vesicles released by either any prokaryotic or eukaryotic cell, or both, with a biological role in cell-to-cell communication. In this work, we characterize the proteomes and nanomechanical properties of EVs released by tissue-culture cell-derived trypomastigotes (mammalian infective stage; (TCT)) and epimastigotes (insect stage; (E)) of Trypanosoma cruzi, the etiologic agent of Chagas disease. EVs of each stage were isolated by differential centrifugation and analyzed using liquid chromatography with tandem mass spectrometry (LC-MS/MS), dynamic lig
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15

Lee, Iksu, Seungbin Kim, Dongjun Lee, Daeun Kim, and Insung Bae. "Dipole Polarization-Induced High Proton Conduction and Aligned Molecular Structures of Nafion Blend Membranes with PVDF and PVDF-Trfe for Enhanced Pemfcs." ECS Meeting Abstracts MA2024-02, no. 43 (2024): 2916. https://doi.org/10.1149/ma2024-02432916mtgabs.

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Proton exchange membrane fuel cells (PEMFCs) have emerged as promising energy generation solutions in various applications, owing to their eco-friendly operation. Perfluorosulfonated polymers like Nafion and Aquivion are preferred choices for PEMs due to their high proton conductivity and excellent stability. However, the bicontinuous structure of Nafion membranes limits proton conductivity, necessitating strategies for enhancement. Methods such as mechanical stretching and magnetic field alignment have been explored to align PEM nanostructures. Mechanically stretched Nafion membranes exhibit
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16

Benedetto, Antonio. "Ionic liquids meet lipid bilayers: a state-of-the-art review." Biophysical Reviews, January 2, 2024. http://dx.doi.org/10.1007/s12551-023-01173-3.

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AbstractIn the past 25 years, a vast family of complex organic salts known as room-temperature ionic liquids (ILs) has received increasing attention due to their potential applications. ILs are composed by an organic cation and either an organic or inorganic anion, and possess several intriguing properties such as low vapor pressure and being liquid around room temperature. Several biological studies flagged their moderate-to-high (cyto)-toxicity. Toxicity is, however, also a synonym of affinity, and this boosted a series of biophysical and chemical-physical investigations aimed at exploiting
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17

Gabbutt, Calum, Wuyi Shen, Jacob Seifert, and Sonia Contera. "AFM nanoindentation reveals decrease of elastic modulus of lipid bilayers near freezing point of water." Scientific Reports 9, no. 1 (2019). http://dx.doi.org/10.1038/s41598-019-55519-7.

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AbstractCell lipid membranes are the primary site of irreversible injury during freezing/thawing and cryopreservation of cells, but the underlying causes remain unknown. Here, we probe the effect of cooling from 20 °C to 0 °C on the structure and mechanical properties of 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) bilayers using atomic force microscopy (AFM) imaging and AFM-based nanoindentation in a liquid environment. The Young’s modulus of elasticity (E) at each temperature for DPPC was obtained at different ionic strengths. Both at 20 mM and 150 mM NaCl, E of DPPC bilayers increases
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18

Nehls, Christian, Marcel Schröder, Thomas Haubenthal, Albert Haas, and Thomas Gutsmann. "The mechanistic basis of the membrane‐permeabilizing activities of the virulence‐associated protein A (VapA) from Rhodococcus equi." Molecular Microbiology, February 3, 2024. http://dx.doi.org/10.1111/mmi.15233.

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AbstractPathogenic Rhodococcus equi release the virulence‐associated protein A (VapA) within macrophage phagosomes. VapA permeabilizes phagosome and lysosome membranes and reduces acidification of both compartments. Using biophysical techniques, we found that VapA interacts with model membranes in four steps: (i) binding, change of mechanical properties, (ii) formation of specific membrane domains, (iii) permeabilization within the domains, and (iv) pH‐specific transformation of domains. Biosensor data revealed that VapA binds to membranes in one step at pH 6.5 and in two steps at pH 4.5 and d
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19

Yan, Bo, Juan Ren, Yue Liu, Huarong Huang, Xi Zheng, and Qingze Zou. "Study of Cholesterol Repletion Effect on Nanomechanical Properties of Human Umbilical Vein Endothelial Cell Via Rapid Broadband Atomic Force Microscopy." Journal of Biomechanical Engineering 139, no. 3 (2017). http://dx.doi.org/10.1115/1.4035260.

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Abnormalities of blood cholesterol concentration are associated with increased risks for vascular disease, especially heart attacks and strokes. As one of the main lipid components of plasma membrane in all mammalian cells, cholesterol has a major impact on the mechanical properties of the membrane of endothelial cells. Although the effect of cholesterol depletion on cell mechanical properties has been studied, no results yet have been reported on quantitative investigation of cholesterol repletion effect. In this study, the cholesterol repletion effect on the nanomechanical properties of huma
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20

Mei, Nanqin, Jingwen Liang, Danielle M. McRae, and Zoya Leonenko. "Localized surface plasmon resonance and atomic force microscopy study of model lipid membranes and their interactions with amyloid and melatonin." Nanotechnology, April 18, 2024. http://dx.doi.org/10.1088/1361-6528/ad403b.

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Abstract Alzheimer’s disease (AD) is a progressive neurodegenerative disorder characterized by the accumulation of amyloid plaques in the brain. The toxicity of amyloid to neuronal cell surfaces arises from interactions between small intermediate aggregates, namely amyloid oligomers, and the cell membrane. The nature of these interactions changes with age and disease progression. In our previous work, we demonstrated that both membrane composition and nanoscale structure play crucial roles in amyloid-membrane interactions. In our previous work, we demonstrated that both membrane composition an
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21

Ruskamo, Salla, Arne Raasakka, Jan Skov Pedersen, et al. "Human myelin proteolipid protein structure and lipid bilayer stacking." Cellular and Molecular Life Sciences 79, no. 8 (2022). http://dx.doi.org/10.1007/s00018-022-04428-6.

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AbstractThe myelin sheath is an essential, multilayered membrane structure that insulates axons, enabling the rapid transmission of nerve impulses. The tetraspan myelin proteolipid protein (PLP) is the most abundant protein of compact myelin in the central nervous system (CNS). The integral membrane protein PLP adheres myelin membranes together and enhances the compaction of myelin, having a fundamental role in myelin stability and axonal support. PLP is linked to severe CNS neuropathies, including inherited Pelizaeus-Merzbacher disease and spastic paraplegia type 2, as well as multiple sclero
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22

Cantero, Miguel, Virginija Cvirkaite-Krupovic, Mart Krupovic, and Pedro J. de Pablo. "Mechanical tomography of an archaeal lemon-shaped virus reveals membrane-like fluidity of the capsid and liquid nucleoprotein cargo." Proceedings of the National Academy of Sciences 120, no. 42 (2023). http://dx.doi.org/10.1073/pnas.2307717120.

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Archaeal lemon-shaped viruses have unique helical capsids composed of highly hydrophobic protein strands which can slide past each other resulting in remarkable morphological reorganization. Here, using atomic force microscopy, we explore the biomechanical properties of the lemon-shaped virions of Sulfolobus monocaudavirus 1 (SMV1), a double-stranded DNA virus which infects hyperthermophilic (~80 °C) and acidophilic (pH ~ 2) archaea. Our results reveal that SMV1 virions are extremely soft and withstand repeated extensive deformations, reaching remarkable strains of 80% during multiple cycles o
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23

Aleksanyan, Mina, Andrea Grafmüller, Fucsia Crea, et al. "Photomanipulation of Minimal Synthetic Cells: Area Increase, Softening, and Interleaflet Coupling of Membrane Models Doped with Azobenzene‐Lipid Photoswitches." Advanced Science, August 31, 2023. http://dx.doi.org/10.1002/advs.202304336.

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AbstractLight can effectively interrogate biological systems in a reversible and physiologically compatible manner with high spatiotemporal precision. Understanding the biophysics of photo‐induced processes in bio‐systems is crucial for achieving relevant clinical applications. Employing membranes doped with the photolipid azobenzene‐phosphatidylcholine (azo‐PC), a holistic picture of light‐triggered changes in membrane kinetics, morphology, and material properties obtained from correlative studies on cell‐sized vesicles, Langmuir monolayers, supported lipid bilayers, and molecular dynamics si
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24

Shtykova, E. V., M. V. Petoukhov, L. A. Dadinova, et al. "Solution Structure, Self-Assembly, and Membrane Interactions of the Matrix Protein from Newcastle Disease Virus at Neutral and Acidic pH." Journal of Virology 93, no. 6 (2018). http://dx.doi.org/10.1128/jvi.01450-18.

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ABSTRACTNewcastle disease virus (NDV) is an enveloped paramyxovirus. The matrix protein of the virus (M-NDV) has an innate propensity to produce virus-like particles budding from the plasma membrane of the expressing cell without recruiting other viral proteins. The virus predominantly infects the host cell via fusion with the host plasma membrane or, alternatively, can use receptor-mediated endocytic pathways. The question arises as to what are the mechanisms supporting such diversity, especially concerning the assembling and membrane binding properties of the virus protein scaffold under bot
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25

sprotocols. "Preparation of tools for lithographically controlled wetting and soft lithography." January 8, 2015. https://doi.org/10.5281/zenodo.13838.

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Authors: Massmiliano Cavallini, Denis Gentili, Pierpaolo Greco, Francesco Valle & Fabio Biscarini ### Abstract This protocol provides the instructions for designing and fabricating stamping tools with features ranging from nanometer to micrometer scale, including the fabrication using commercial tools such as compact disks or digital video disks. In particular the reported procedures are oriented towards the tools fabrication for lithographically controlled wetting and soft lithography. Because the versatility of these methods that has almost no restrictions concerning the materials used f
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