Tesis: "Mice as laboratory animals"

1

Agarwal, Rajat. "A model for minimizing cost for housing laboratory mice." [Gainesville, Fla.] : University of Florida, 2003. http://purl.fcla.edu/fcla/etd/UFE0001241.

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2

Hsu, Charlie Chun. "Isolation, characterization, and diagnosis of murine noroviruses, a newly recognized pathogen of mice." Diss., Columbia, Mo. : University of Missouri-Columbia, 2007. http://hdl.handle.net/10355/4790.

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Thesis (Ph. D.)--University of Missouri-Columbia, 2007. "December 2007" The entire dissertation/thesis text is included in the research.pdf file; the official abstract appears in the short.pdf file (which also appears in the research.pdf); a non-technical general description, or public abstract, appears in the public.pdf file. Vita. Includes bibliographical references.

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3

Filipovska-Naumovska, Emilija. "Development of methods for detection and eradication of mouse parvovirus from a laboratory mouse colony." Thesis, Filipovska-Naumovska, Emilija (2007) Development of methods for detection and eradication of mouse parvovirus from a laboratory mouse colony. PhD thesis, Murdoch University, 2007. https://researchrepository.murdoch.edu.au/id/eprint/676/.

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The mouse parvovirus designated MPV can infect laboratory mice and affect the humoral and cellular immune response of infected mice, reducing their value for biomedical and medical research. The development and maintenance of MPV-free mouse colonies for biomedical research is therefore essential and requires routine monitoring of the infection status of mice, using serological surveillance procedures. Recent experience in the Animal Resources Centre (ARC), a major supplier of mice to the medical research community in Australia, was that MPV infection was present but was not detectable with the serological tests that were then in routine use. This thesis reports the development of a polymerase chain reaction (PCR) assay for the detection of the MPV in the ARC mouse colonies, the genetic characteristics of the strain of MPV detected, the development of a recombinant virus protein that provided a suitable antigen for enzyme-linked immunosorbent assay (ELISA) and a Western immunoblot (WIB) assay for the detection of MPV antibodies, and use of these various assays to determine aspects of the epidemiology and pathogenicity of the infection that were critical to the eradication of virus infection and future immunological surveillance to ensure the absence of infection. The recombinant protein produced as an antigen was a biotinylated fusion protein, a truncated capsid protein of the strain of MPV detected in the ARC, and was produced using the PinpointTM vector and with expression in Escherichia coli. The protein was produced as an insoluble intracellular product within inclusion bodies and was solubilised using urea and purified. The purified protein was utilised as an antigen for ELISA and the WIB assays to detect virus antibody in infected mice. The outbreak of MPV infection in the ARC was used as an unique opportunity for assessment of the seroprevalence of MPV-1 infection in a large laboratory mouse colony and to utilise this data to determine the sampling size needed to reliably detect MPV-1 infection within such large laboratory mouse colonies. An overall seroprevalence of 16.5% was detected using the developed serological tests, but considerable variation in prevalence was detected in different mouse strains. The response to MPV infection of 4 different but common strains of mice was determined as a basis for developing appropriate surveillance procedures and the selection of appropriate sentinel animals. The effect of infection of these strains at different ages was also investigated. Virus replication was detected in tissues of all the mice strains infected (outbred ARC(s) and inbred C57BL/6JArc, BALB/c and BALB/c-Foxn1nu/Arc) as juveniles and adults, with the exception of C57BL/6JArc inoculated as adults. However, while seroconversion in mice inoculated as juveniles and adults was detected in ARC(s) and C57BL/6JArc mice, it was not detected in BALB/c mice. The high rate of seroconversion to MPV, the early and prolonged development of an immune response, and the lack of age differences in their susceptibility indicated that ARC(s) mice would provide reliable sentinels for the detection of MPV. The genomic nucleotide sequence of the ARC strain, excluding the terminal palindromic regions and the predicted amino acid sequences of the non-structural and structural proteins was determined. This strain was very similar (98-99% nucleotide identity) to the previously described MPV strains MPV-1a, MPV-1b and MPV -1c. The similarity suggested there were unlikely to be significant antigenic differences in the proteins of the ARC strain and those strains of MPV reported previously.

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4

Filipovska-Naumovska, Emilija. "Development of methods for detection and eradication of mouse parvovirus from a laboratory mouse colony." Filipovska-Naumovska, Emilija (2007) Development of methods for detection and eradication of mouse parvovirus from a laboratory mouse colony. PhD thesis, Murdoch University, 2007. http://researchrepository.murdoch.edu.au/676/.

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The mouse parvovirus designated MPV can infect laboratory mice and affect the humoral and cellular immune response of infected mice, reducing their value for biomedical and medical research. The development and maintenance of MPV-free mouse colonies for biomedical research is therefore essential and requires routine monitoring of the infection status of mice, using serological surveillance procedures. Recent experience in the Animal Resources Centre (ARC), a major supplier of mice to the medical research community in Australia, was that MPV infection was present but was not detectable with the serological tests that were then in routine use. This thesis reports the development of a polymerase chain reaction (PCR) assay for the detection of the MPV in the ARC mouse colonies, the genetic characteristics of the strain of MPV detected, the development of a recombinant virus protein that provided a suitable antigen for enzyme-linked immunosorbent assay (ELISA) and a Western immunoblot (WIB) assay for the detection of MPV antibodies, and use of these various assays to determine aspects of the epidemiology and pathogenicity of the infection that were critical to the eradication of virus infection and future immunological surveillance to ensure the absence of infection. The recombinant protein produced as an antigen was a biotinylated fusion protein, a truncated capsid protein of the strain of MPV detected in the ARC, and was produced using the PinpointTM vector and with expression in Escherichia coli. The protein was produced as an insoluble intracellular product within inclusion bodies and was solubilised using urea and purified. The purified protein was utilised as an antigen for ELISA and the WIB assays to detect virus antibody in infected mice. The outbreak of MPV infection in the ARC was used as an unique opportunity for assessment of the seroprevalence of MPV-1 infection in a large laboratory mouse colony and to utilise this data to determine the sampling size needed to reliably detect MPV-1 infection within such large laboratory mouse colonies. An overall seroprevalence of 16.5% was detected using the developed serological tests, but considerable variation in prevalence was detected in different mouse strains. The response to MPV infection of 4 different but common strains of mice was determined as a basis for developing appropriate surveillance procedures and the selection of appropriate sentinel animals. The effect of infection of these strains at different ages was also investigated. Virus replication was detected in tissues of all the mice strains infected (outbred ARC(s) and inbred C57BL/6JArc, BALB/c and BALB/c-Foxn1nu/Arc) as juveniles and adults, with the exception of C57BL/6JArc inoculated as adults. However, while seroconversion in mice inoculated as juveniles and adults was detected in ARC(s) and C57BL/6JArc mice, it was not detected in BALB/c mice. The high rate of seroconversion to MPV, the early and prolonged development of an immune response, and the lack of age differences in their susceptibility indicated that ARC(s) mice would provide reliable sentinels for the detection of MPV. The genomic nucleotide sequence of the ARC strain, excluding the terminal palindromic regions and the predicted amino acid sequences of the non-structural and structural proteins was determined. This strain was very similar (98-99% nucleotide identity) to the previously described MPV strains MPV-1a, MPV-1b and MPV -1c. The similarity suggested there were unlikely to be significant antigenic differences in the proteins of the ARC strain and those strains of MPV reported previously.

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5

Moreira, Virgínia Barreto [UNESP]. "Eficiência reprodutiva e comportamento parental de camundongos isogênicos e heterogênicos produzidos em ambiente modificado." Universidade Estadual Paulista (UNESP), 2015. http://hdl.handle.net/11449/126631.

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Made available in DSpace on 2015-08-20T17:10:17Z (GMT). No. of bitstreams: 0 Previous issue date: 2015-05-25. Added 1 bitstream(s) on 2015-08-20T17:25:37Z : No. of bitstreams: 1 000841528.pdf: 469659 bytes, checksum: 2dd38d332ff2b50df196e07151da31a3 (MD5) O objetivo deste estudo foi avaliar a preferência e efeito do fornecimento de materiais de nidificação sobre o desempenho reprodutivo de camundongos isogênicos da linhagem BALB/c e da linhagem heterogênica Swiss em sistema de acasalamento intensivo monogâmico. Primeiro foi realizado um estudo para avaliar a preferência dos camundongos pelo material oferecido para nidificação. Utilizou-se um sistema composto de quatro gaiolas, com livre acesso a água e ração, interligados por tubos de PVC que permitiam que os animais se locomovessem entre todas as gaiolas. Quatro tipos de materiais foram oferecidos para a construção do ninho: algodão, gaze, rolinho de papelão, e touca de polipropileno descartável. Cada um dos quatro materiais foi oferecido simultaneamente em uma das quatro gaiolas que compunham o sistema. Foram usados 10 sistemas iguais e cada um abrigou um casal da linhagem BALB/c, desde os 28 dias de idade até o terceiro ciclo reprodutivo. Os mais encontrados na confecção do ninho, em ordem decrescente, foram a touca, rolinho, algodão e gaze (P< 0,0083). Com base nestes resultados foram selecionados dois tipos de materiais para fornecimento aos animais no experimento 2. Embora o rolinho tenha sido o segundo material mais utilizado optou-se pelo algodão devido a inviabilidade de fornecimento do item durante todo o período de duração do experimento 2. O segundo experimento avaliou a eficiência reprodutiva em cinco ciclos reprodutivos do nascimento até o desmame. Usou-se 60 casais de irmãos completos da linhagem BALB/c (isogênica) e 60 casais formados por acasalamentos aleatórios da linhagem Swiss (heterogênica) de padrão sanitário controlado, criados e mantidos em ambiente padronizado. Eles foram distribuídos, num delineamento inteiramente casualizado, em arranjo fatorial 2x2 (duas linhagens em alojamento com ou sem material para nidificação). Como forma de ... The objective of this study was to evaluate the preference and effect of nesting materials provision on performance of inbred mice of the BALB/c strain and of heterogenic Swiss in intensive monogamous mating system. Firstly, a study was conducted to evaluate the preference of mice for the material offered for nesting. A system composed of four cages was used, with free access to water and food, connected by PVC tubing, which allowed animal displacement among all cages. Four types of materials were available for construction of the nest: cotton, gauze, cardboard rolls, and disposable polypropylene cap. Each of the four materials was offered simultaneously in one of four cages that formed the system. Ten identical system were used and each one housed a BALB/c couple, from 28 days of age up to the third reproductive cycle. The most commonly found materials in nest making were cap, roll, cotton and gauze (P <0.0083). Based on these results, two types of materials were selected to be offered to the animals in experiment 2. Although the roll was the second most used material, we chose cotton due to unfeasibility supplying of the item throughout the duration of the second experiment. The second experiment evaluated the reproductive efficiency in five reproductive cycles from birth to weaning. Sixty BALB / c full siblings pairs (inbred) and 60 pairs formed by random mating of Swiss strain (outbred), bred and maintained in a standardized environment were used. They were distributed in a completely randomized design in a 2x2 factorial arrangement (two strains in housing with or without nesting material). As a way of cage enrichment, polypropylene disposable cap cut into 8 pieces of approximately 1 cm each and one piece of cotton about 3 g were used after being previously packaged and autoclaved . The following characteristics were evaluated: age at first parturition, litter intervals, pre-weaning mortality and ...

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6

Moreira, Virgínia Barreto 1974. "Eficiência reprodutiva e comportamento parental de camundongos isogênicos e heterogênicos produzidos em ambiente modificado /." Botucatu, 2015. http://hdl.handle.net/11449/126631.

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Orientador: Ana Silvia Alves Meira Tavares Moura Banca: Simone Fernandes Banca: Valderez Bastos Valero-Lapckick Banca: Denose Rangel da Silva Sartori Banca: Luiz Edivaldo Pezzato Resumo: O objetivo deste estudo foi avaliar a preferência e efeito do fornecimento de materiais de nidificação sobre o desempenho reprodutivo de camundongos isogênicos da linhagem BALB/c e da linhagem heterogênica Swiss em sistema de acasalamento intensivo monogâmico. Primeiro foi realizado um estudo para avaliar a preferência dos camundongos pelo material oferecido para nidificação. Utilizou-se um sistema composto de quatro gaiolas, com livre acesso a água e ração, interligados por tubos de PVC que permitiam que os animais se locomovessem entre todas as gaiolas. Quatro tipos de materiais foram oferecidos para a construção do ninho: algodão, gaze, rolinho de papelão, e touca de polipropileno descartável. Cada um dos quatro materiais foi oferecido simultaneamente em uma das quatro gaiolas que compunham o sistema. Foram usados 10 sistemas iguais e cada um abrigou um casal da linhagem BALB/c, desde os 28 dias de idade até o terceiro ciclo reprodutivo. Os mais encontrados na confecção do ninho, em ordem decrescente, foram a touca, rolinho, algodão e gaze (P< 0,0083). Com base nestes resultados foram selecionados dois tipos de materiais para fornecimento aos animais no experimento 2. Embora o rolinho tenha sido o segundo material mais utilizado optou-se pelo algodão devido a inviabilidade de fornecimento do item durante todo o período de duração do experimento 2. O segundo experimento avaliou a eficiência reprodutiva em cinco ciclos reprodutivos do nascimento até o desmame. Usou-se 60 casais de irmãos completos da linhagem BALB/c (isogênica) e 60 casais formados por acasalamentos aleatórios da linhagem Swiss (heterogênica) de padrão sanitário controlado, criados e mantidos em ambiente padronizado. Eles foram distribuídos, num delineamento inteiramente casualizado, em arranjo fatorial 2x2 (duas linhagens em alojamento com ou sem material para nidificação). Como forma de ... Abstract: The objective of this study was to evaluate the preference and effect of nesting materials provision on performance of inbred mice of the BALB/c strain and of heterogenic Swiss in intensive monogamous mating system. Firstly, a study was conducted to evaluate the preference of mice for the material offered for nesting. A system composed of four cages was used, with free access to water and food, connected by PVC tubing, which allowed animal displacement among all cages. Four types of materials were available for construction of the nest: cotton, gauze, cardboard rolls, and disposable polypropylene cap. Each of the four materials was offered simultaneously in one of four cages that formed the system. Ten identical system were used and each one housed a BALB/c couple, from 28 days of age up to the third reproductive cycle. The most commonly found materials in nest making were cap, roll, cotton and gauze (P <0.0083). Based on these results, two types of materials were selected to be offered to the animals in experiment 2. Although the roll was the second most used material, we chose cotton due to unfeasibility supplying of the item throughout the duration of the second experiment. The second experiment evaluated the reproductive efficiency in five reproductive cycles from birth to weaning. Sixty BALB / c full siblings pairs (inbred) and 60 pairs formed by random mating of Swiss strain (outbred), bred and maintained in a standardized environment were used. They were distributed in a completely randomized design in a 2x2 factorial arrangement (two strains in housing with or without nesting material). As a way of cage enrichment, polypropylene disposable cap cut into 8 pieces of approximately 1 cm each and one piece of cotton about 3 g were used after being previously packaged and autoclaved . The following characteristics were evaluated: age at first parturition, litter intervals, pre-weaning mortality and ... Doutor

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7

Iñiguez, Sergio Diaz. "The effects of acute posttraining injections of cocaine on spatial memory in C57BL/6 mice." CSUSB ScholarWorks, 2007. https://scholarworks.lib.csusb.edu/etd-project/3244.

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The purpose of this study was to investigate the effects of cocaine on spatial memory consolidation using the Morris water maze. Specifically, male and female C57BL/6 mice were trained on a spatial water task, and then administered a single posttraining injection of saline or cocaine (1.25, 2.5, 5.0, or 20.0 mg/kg).

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8

Berting, Jennifer Irene. "Inbreeding effects on physiological responses to chronic hypoxia in mice (Mus musculus) /." Electronic version (PDF), 2007. http://dl.uncw.edu/etd/2007-3/bertingj/jenniferberting.pdf.

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Jyotika, Jigyasa. "Deletion of the Bax gene severely impairs sexual behavior and modestly impairs motor function in mice." Connect to this title, 2008. http://scholarworks.umass.edu/theses/158/.

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10

Migdalska, Anna Marta. "Modelling human genetic disorders in mice." Thesis, University of Cambridge, 2012. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.610341.

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11

Augustsson, Hanna. "Ethoexperimental studies of behaviour in wild and laboratory mice : risk assessment, emotional reactivity and animal welfare /." Uppsala : Dept. of Large Animal Clinical Sciences, Swedish Univ. of Agricultural Sciences, 2004. http://epsilon.slu.se/v174.pdf.

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12

Wang, Bin. "Functional studies of QRF-1 /." Digital version accessible at:, 2000. http://wwwlib.umi.com/cr/utexas/main.

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13

Xiang, Li. "Metabolomics study of regulatory effects of exercise training on db/db type 2 diabetic mice." HKBU Institutional Repository, 2018. https://repository.hkbu.edu.hk/etd_oa/489.

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Type 2 diabetes mellitus (T2DM) is mainly caused by genetic modifications and inappropriate life styles. The complexity of T2DM has brought us challenges for a comprehensive understanding of altered metabolic pathways that contributing to the development of T2DM. Therefore, a comprehensive metabolic analysis is needed. To date, taking regular exercise is a common and effective therapeutic way known to antagonize the metabolic disorders of T2DM. However, the regulatory effects of exercise on T2DM or T2DM induced complications have not been clearly characterized. Here, we present the effect of physical activity on biochemical changes in diabetic db/db mice in plasma, urine, skeletal muscle and kidney samples. Based on liquid chromatography coupled with high resolution Orbitrap mass spectrometry (LC-MS) and gas chromatography coupled with mass spectrometry (GC-MS), two major approaches, untargeted and targeted metabolomics studies, have been developed to delineate metabolic signatures in various kinds of biofluid and tissue samples. Targeted quantification methods on acylcarnitines and acyl-CoA have been developed. Untargeted metabolomics analysis by GC-MS and LC-MS have also been developed to draw a more comprehensive view of the metabolic changes in response to T2DM and exercise on db/db diabetic mice. The transcript expressions of mRNA in pathways of interest have also been measured to confirm the hypothesis. Firstly, a targeted quantification method of acylcarnitines by using high resolution parallel reaction monitoring (PRM) on LC-MS platform has been developed. A total of 117 acylcarnitines were detected from plasma and urine samples. The application of targeted profiling of acylcarnitines in db/m+ control and db/db diabetic mice indicated incomplete amino acid and fatty acid oxidation in diabetic mice. Interestingly, the reduction of medium odd-numbered chain acylcarnitines in urine samples was firstly observed between db/m+ and db/db mice. The high resolution PRM method makes it possible to monitor the widespread metabolic changes of the acylcarnitines in response to stimuli. Besides, the accurate MS and MS/MS spectra data of the 117 acylcarnitines could be used as mass spectrometric resources for the identification of acylcarnitines. In addition to targeted metabolomics analysis, untargeted metabolomics profiling analysis in plasma samples indicated that db/db diabetic mice may be more susceptible to exercise for energy expenditure. Interestingly, all the results from plasma, skeletal muscle and kidney samples may demonstrate that physical activity could mitigate insulin resistance in T2DM mice through improving fatty acid β-oxidation (FAO) and eliminating overloaded intermediate which contribute to insulin resistance. Specifically, the results from kidney samples demonstrated that exercise exhibit beneficial effect in reducing hyperlipidemia, expression levels of inflammatory markers (TNFα, IL-6 and COX2) and fibrosis markers (Collagen 1), and alleviating diabetic nephropathy (DN) induced mesangial expansion in kidneys of diabetic mice. The results of metabolic changes in kidney of db/db mice revealed that the accumulation of acyl-CoA, phospholipids and hydroxylated acylcarnitines were substantially ameliorated by exercise, and the reduction of important enzymes CTP1α and Acadl in FAO were partially reversed. In addition, branched-chain amino acids (BCAA) metabolism which positively related to inflammation (TNFα) was down-regulated in DN mice by exercise. What’s more, the accumulation of uric acid, which contributes to inflammation and tubulointestitial fibrosis in kidney disease, together with its six precursors have also been substantially reduced. The results in kidney samples demonstrated that in addition to beneficial effect in alleviating lipotoxicity through improving FAO efficiency, exercise also ameliorated diabetic induced inflammation and fibrosis via promoting BCAA catabolism and accelerating the elimination of uric acid. Together, the mass spectrometry-based metabolomics study is a powerful tool to investigate the regulatory effect of exercise on complex metabolic diseases. The results may provide informative insights into the underlying the mechanism of exercise on T2DM and T2DM induced complications.

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14

Buonincontri, Guido. "Advanced MRI for cardiac assessment in mice." Thesis, University of Cambridge, 2014. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.648679.

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15

Brown, Steven J. "Immunological studies of a glycosylation based mouse model of colitis /." Connect to thesis, 2004. http://eprints.unimelb.edu.au/archive/00000788.

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Thesis (Ph.D.)--University of Melbourne, Dept. of Gastroenterology and the Immunology Research Centre St. Vincents Hospital & Dept of Medicine, 2004. Typescript (photocopy). Includes bibliographical references (leaves 309-343).

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16

歐穗欣 and Sui-yan Au. "Characterization of the mouse myosin va cargo-binding domain." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2002. http://hub.hku.hk/bib/B31227107.

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17

Lai, Yeuk-yu. "Characterization of lymphocyte development in young and aged mice." Click to view the E-thesis via HKUTO, 2003. http://sunzi.lib.hku.hk/hkuto/record/B31971076.

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18

周燕華 and Yin-wah Eva Chow. "A study of spontaneously developing malignant lymphoma in SJL/N mice by immunoenzymatic methods." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 1986. http://hub.hku.hk/bib/B31969549.

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19

Chow, Yin-wah Eva. "A study of spontaneously developing malignant lymphoma in SJL/N mice by immunoenzymatic methods." [Hong Kong : University of Hong Kong], 1986. http://sunzi.lib.hku.hk/hkuto/record.jsp?B12324541.

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20

Kwan, Tin-fu. "Lymphocyte development in collagen-induced arthritis mice." Click to view the E-thesis via HKUTO, 2003. http://sunzi.lib.hku.hk/hkuto/record/B31971064.

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21

關天富 and Tin-fu Kwan. "Lymphocyte development in collagen-induced arthritis mice." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2003. http://hub.hku.hk/bib/B31971064.

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22

Gliddon, Briony Lee. "Enzyme replacement therapy in a murine model of mucopolysaccharidosis type IIIA /." Title page, contents and abstract only, 2002. http://web4.library.adelaide.edu.au/theses/09PH/09phg5595.pdf.

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23

Stepp, Phillip W. "The effects of hypotyroidism, the acute inflammatory response, and caloric restriction on neurogensis and behavior in mice /." free to MU campus, to others for purchase, 2004. http://wwwlib.umi.com/cr/mo/fullcit?p3164543.

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24

Tan, Ju Chiat Graduate School of Biomedical Engineering Faculty of Engineering UNSW. "Investigation of abnormal cardiac function in murine models of hypocontractility and hypercontractility." Awarded by:University of New South Wales. Graduate School of Biomedical Engineering, 2006. http://handle.unsw.edu.au/1959.4/28879.

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Heart failure has a significant impact on mortality and morbidity. Dilated cardiomyopathy (DCM) is the third most common cause of heart failure and the most common reason for heart transplantation. Familial DCM is known to be caused by mutations in the LMNA gene encoding lamins A and C. New methods to enhance cardiac contractility would be beneficial in the treatment or prevention of heart failure. The focus of this thesis was to evaluate the mechanisms of altered contractility in two mouse models: the LMNA knockout model (homozygous, Lmna-/-; heterozygous, Lmna+/-) generated by targeted deletion of the lmna gene, and the model of enhanced contractility due to cardiac alpha1A-adrenergic receptor (???1A-AR) overexpression (A1A1). Previous studies have found altered nuclear-desmin connections in lamin A/C deficient mice. It was proposed that these alterations result in ???defective force transmission???, which leads to DCM. Studies in this thesis have supported this hypothesis. Studies of isolated single cardiomyocytes from mice aged 4-6 weeks demonstrated abnormal cell morphology and contractile dysfunction in Lmna-/- cardiomyocytes, while Lmna+/- cells showed no overt phenotype. Excitation-contraction coupling experiments and forcecalcium studies in skinned fibers excluded altered calcium kinetics as a primary cause of DCM in this model, but there was evidence of reduced sarcomere numbers and reduced sarcomere lengths as a contributor to reduce force generation in Lmna-/- and Lmna+/- mice. Previous in vivo studies showed that A1A1 mice had enhanced contractility with the absence of hypertrophy. Studies on isolated single cardiomyocytes from A1A1 mice aged 8-12 weeks showed reduced contractility in the absence of ???1A-AR stimulation, but an exaggerated response to ???1A-AR stimulation. In contrast isolated isovolumic Langendorff perfused A1A1 hearts without ???1A-AR stimulation replicated the enhanced contractility observed in vivo. These studies are consistent with down-regulation of contractility due to the hyperactivity of the overexpressed ???1A-AR in vivo, which only becomes evident in isolated cells without ???1A-AR stimulation due to the loss of functional receptor numbers during isolation. Sufficient spontaneously active ???1A-ARs are preserved in the isolated Langendorff heart preparation to ensure maximum contractility driven by increase calcium release.

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25

Naik, Shalin Hemant. "Distinct precursors of the dendritic cell subtypes /." Connect to thesis, 2006. http://eprints.unimelb.edu.au/archive/00001885.

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26

Tsang, Hon-man. "Studies of Mll-Een fusion gene in a conditional mouse model of human leukemia." Click to view the E-thesis via HKUTO, 2007. http://sunzi.lib.hku.hk/HKUTO/record/B39558034.

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27

McEwen, Kirsten Rose. "Epigenetic regulation of imprinted loci in the mouse." Thesis, University of Cambridge, 2011. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.609297.

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28

Wong, Ka-yan Karen, and 黃嘉欣. "The functional interaction of mouse secretin and angiotensin II receptors." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2010. http://hub.hku.hk/bib/B46087187.

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29

黎若愚 and Yeuk-yu Lai. "Characterization of lymphocyte development in young and aged mice." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2003. http://hub.hku.hk/bib/B31971076.

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30

楊可儀 and Ho-yee Yeung. "Study on the function and regulation of stanniocalcin in mouse neuroblastoma cells." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2003. http://hub.hku.hk/bib/B31245043.

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31

Torres, Cano Alejo. "WT1 in heart and gonad development: a crucial gene for cell plasticity and differentiation." Doctoral thesis, Universitat de Barcelona, 2021. http://hdl.handle.net/10803/673447.

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Wt1 is a complex gene that encodes a protein whose best described function is to act as a transcription factor. Wt1 plays a crucial role in several organ developments, including kidneys, liver, heart or gonads. Moreover, Wt1 is implicated in the etiology of a set of syndromes and diseases ranging from cancer to disorders of sex development (DSD). Additionally, Wt1 has been described to play a role in adult homeostasis. In this thesis, I focused on Wt1 role in development, specifically in gonad and heart morphogenesis. For this purpose, I used two previously described Cre mouse models to generate two different Wt1KOs. The heart is the first organ being formed and it is primarily composed of three layers: the endocardium, the myocardium and the epicardium. The epicardium is a mesothelial layer of cells covering the vertebrate heart surface. Epicardial cells give rise to epicardial derived cells (EPDCs), progenitors of crucial cell types in heart development like vascular smooth muscle cells and cardiac fibroblasts. Moreover, the epicardium contributes to heart morphogenesis by secreting paracrine factors. After myocardial infarction, the epicardium embryonic genetic signature is reactivated. Thus, understanding the mechanisms behind epicardial development constitutes a topic of general interest. The epicardial genetic program has been previously characterized and the Wt1 role in the regulation of this program has been established. One of the pathways modulated during epicardium development is the BMP4 pathway. The results of this thesis demonstrate that WT1 directly regulates Bmp4 expression. Additionally, exposed results demonstrate how the BMP4 pathway is crucial for epicardial cell maturation, through the regulation of cell morphology, from a cuboidal to a squamous cell shape, epicardial proliferation, and transcriptomic changes. Finally, the data shown in this work indicated that changes and mechanisms described for epicardium may be a phenomenon extrapolated to other mesotheliums like the lung mesothelium. To better study Wt1 role in the development of other organs, we have characterized the recombination activity of a Wt1Cre mouse model using two different reporter mouse models, the R26RmTmG and the R26RtdRFP. Results demonstrated that Wt1Cre is efficiently activated in hearts and gonads but not in other organs generated from the genital ridge, like the kidney or the adrenal gland. Taking advantage of this, the Wt1Cre mouse model was used to generate a new Wt1KO: the Wt1Cre; Wt1Loxp/GFP. Wt1Cre; Wt1Loxp/GFP mice show a partial embryonic lethality, potentially caused by defects in heart development, but some of them reach adulthood, becoming an ideal model to investigate Wt1 role from embryonic to adult stages. Therefore, we decided to study Wt1role in gonad development in Wt1Cre; Wt1Loxp/GFP mice. Sex development is a complex and coordinated process that starts with the differentiation of the bipotential gonads. WT1 mutations or haploinsufficiency have been reported in different syndromes and conditions linked to DSD. The study of WT1 role in embryonic development and the impact on adult sex development has been hampered by the complete gonadal agenesis or embryonic lethality presented by other Wt1KO mouse models. The results presented demonstrate a sharp reduction in Wt1 levels in Wt1Cre; Wt1Loxp/GFP gonads since the bipotential stage. This causes, in the adult, the formation of small, atrophic gonads, a hermaphroditism of the genital tract and external ambiguous genitalia. Besides, the data reported in this thesis demonstrates that Wt1Cre; Wt1Loxp/GFP mice present an impaired gonad embryonic development due to the lack of differentiation of the main cell lineages responsible for gonad development and function: supportive cells, steroidogenic cells and primordial germ cells (PGCs). Finally, the observed sub-lethality in Wt1Cre; Wt1Loxp/GFP mice is explained by the observation of a severe heart developmental impairment in a portion of Wt1Cre; Wt1Loxp/GFP embryos, whereas others show no apparent heart defects at embryonic stages. Equally, an histological study performed on Wt1Cre; Wt1Loxp/GFP mice also described changes in the spleen and brown adipose tissue. In summary, our results indicate the importance of morphological changes epicardial cells undergo during development, a process regulated by Wt1 modulation of the BMP4 pathway. In addition, the generation and characterization of the Wt1Cre; Wt1Loxp/GFP mouse renders an informative and useful tool to study Wt1 role beyond embryonic stages and provide a more accurate frame for the understanding of results previously obtained when using the Wt1Cre mouse model.

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32

Li, Siu-ming Ian. "A study of cyclophosphamide on dextran sulfate sodium-induced ulcerative colitis in mice." Click to view the E-thesis via HKUTO, 2004. http://sunzi.lib.hku.hk/hkuto/record/B31971994.

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Yu, Chun-I. Palucka Karolina Banchereau Jacques. "Humanized mice to test vaccination against influenza virus via dendritic cells." Waco, Tex. : Baylor University, 2008. http://hdl.handle.net/2104/5184.

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Thesis (Ph.D.)--Baylor University, 2008. In abstract the '2' and '-/-' in NOD-SCID-[beta]2m-/- is superscript. In abstract the '+' after CD34 and CD8 is superscript. In abstract the '-' and '+' in CD45RA-CD27+CD4+ are superscript. Includes bibliographical references (p. 103-123).

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Li, Yuk-yin. "Adrenomedullin in the rat reproductive systems and the changes of the gene expression of adrenomedullin and its receptor components during ageing." Click to view the E-thesis via HKUTO, 2008. http://sunzi.lib.hku.hk/HKUTO/record/B3955692X.

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35

Liu, Yan. "The immunosuppressive effects of Triptolide and Rapamycin on mouse model of cardiac transplantation." Click to view the E-thesis via HKUTO, 2007. http://sunzi.lib.hku.hk/hkuto/record/B39793904.

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36

陳卓榮 and Cheuk-wing Wilson Chan. "Molecular basis for increased bone formation in a mouse expressing mutant collagen X." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2003. http://hub.hku.hk/bib/B31227132.

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Fisher, Rosie. "Utrophin in therapy of Duchenne muscular distrophy." Thesis, University of Oxford, 2001. http://ora.ox.ac.uk/objects/uuid:192fbccd-d037-4ce8-b1cd-0315afe1860d.

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Clifford, Adrianne Brown. "Tumor Associated Macrophages in a MaFIA Mouse Model." Diss., CLICK HERE for online access, 2006. http://contentdm.lib.byu.edu/ETD/image/etd1427.pdf.

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Li, Siu-ming Ian, and 李紹銘. "A study of cyclophosphamide on dextran sulfate sodium-induced ulcerative colitis in mice." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2004. http://hub.hku.hk/bib/B31971994.

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40

Slobedman, Barry. "Molecular analysis of herpes simplex virus type 1 latency in experimentally infected mice /." Title page, contents and abstract only, 1994. http://web4.library.adelaide.edu.au/theses/09PH/09phs634.pdf.

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Thesis (Ph. D.)--University of Adelaide, Dept. of Microbiology and Immunology, 1995? Copies of author's previously published articles inserted inside back cover. Includes bibliographical references (leaves 137-179).

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41

McLean, Fiona Hamilton. "The impact of a high-fat diet on memory in mice." Thesis, University of Aberdeen, 2016. http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?pid=231763.

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Obesity and type II diabetes are associated with dementia and Alzheimer's disease. A high-fat diet induces memory deficits in rodents, however, complex episodic-like memory, has not been tested. Episodic memory is the recollection of events using a “what-where-when/which” experience and is the first memory to be compromised in Alzheimer's disease. To identify a link between a high-fat diet and episodic memory, 12 week old, male, C57Bl/6 mice, were fed a semi purified high-fat or low-fat diet ad libitum and tested with object-place-context (episodic-like), novel object recognition, object-place (spatial) and object-context (contextual) memory tasks for up to 2 weeks. A separate group of animals were fed a high-fat diet for 1 week followed by a low-fat diet for 1 week. Animals were killed after 3 days, 1 week or 2 weeks on diet. Brains were kept frozen until the hippocampus was dissected and proteomics performed. Further studies were carried out in rat primary hippocampal cell cultures to investigate the impact of different fatty acids on neuronal dendritic morphology. We found that episodic-like memory is compromised after only one day of a high fat diet together with spatial and contextual tasks. The ability to carry out the novel object recognition test remained intact. Proteomic analysis of hippocampal tissue revealed changes in a number of proteins associated with metabolism, cell stress, cell signalling, inflammation and the cytoskeleton. High-fat diet induced changes were reversed by a low-fat diet. Hippocampal neuron cultures showed that long chain saturated fatty acid palmitic acid, a component of the high-fat diet used in the behavioural and proteomic studies, caused reduced dendritic arborisation whist n-3 polyunsaturated fat docosahexaenoic acid negated these effects. These data link high-fat diet to indices of hippocampal neuronal damage and memory deficits and have implications for the link between diet, obesity and cognitive decline.

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42

Ríos, Kristjánsson Juan Gabriel. "The Effect of intermittent hypobaric hypoxia and exercise on the recovery of induced skeletal muscle damage in trained laboratory rats: performance evaluation, plasma markers and M. Soleus differential gene expression = El efecto de hipoxia hipobárica intermitente y ejercicio de la recuperación del daño muscular esquelético inducido en ratas de laboratorio entrenadas: evaluación de rendimiento, marcadores plasmáticos y expresión génica diferencial del M. Soleus." Doctoral thesis, Universitat de Barcelona, 2016. http://hdl.handle.net/10803/402579.

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One of the key players in the regenerating injured skeletal muscle fibers are their surrounding satellite cells (specific stem cells), arranged between the basal lamina and the sarcolemma, in relatively few numbers per fiber. The satellite cells, which are myogenic precursor cells are activated during injury to fuse and mature into the injured fiber. Some studies have demonstrated that exercise and hypoxia facilitate the activation and migration into the blood circulation, moving more stem cells to an injured or activated area which in turn aids the repairing of the injury. The aim of this thesis was to study the regeneration profile over fourteen days after an eccentric-exercise induction of skeletal muscle damage in treadmill-trained laboratory rat, submitting them to series of daily (4 h) intermittent hypobaric hypoxia (with the simulated altitude of 4000 m) with or without the combination of light exercise as part of their rehabilitation. The rats could potentially react differently to the exercise training prior and post the induction of muscle damage, and furthermore, be differently susceptible to the injury-induction protocol, influenced by their training. This premise led to the formation of the rat AEY performance score, throughout the study, as a complementary tool for the data acquired during the injury regeneration. The regeneration was profiled four times over the recovery fourteen days from the injury induction via the concentration of myoglobin and creatine kinase (CK-MM) plasma markers and differential gene expression analysis in m. soleus, which is generally considered to be one of the most susceptible muscle to eccentric-exercise injury when running downhill. The plasma marker results indicate that the effect of the injury-induction protocol was very short lived, as after the significantly different peak concentrations for the control (passive recovery) and they hypoxia groups (sessions of intermittent hypoxia during recovery) 1 day post injury, had dropped down to basal level again 3 days post injury. However, the hypoxia+exercise group (sessions of intermittent hypoxia followed by light exercise during recovery) had a distinct profile with its slowly rising peak 3 days post injury and then dropping down again. Still, this profile was only significant for the CK-MM measurements, whilst myoglobin did not show any significant change following injury. It is clear that the hypoxia-exercise conditions cause different physiological reactions to the injury. However, the level of the injury would need to be greater in order to obtain a more significant pattern and to be able to interpret if the pattern is reflecting potentially beneficial effects or not. The differential gene expression analysis also indicates that the injury induction needed to have been greater to achieve differential expression. The hypoxia and hypoxia+exercise profiles are not parallel to each other, still the data also suggest that the hypoxia sessions could have been more effective. Therefore, it is difficult to give a general conclusion to which hypoxia or hypoxia+exercise facilitate the injury regeneration to a higher degree. The certain fact is that there are ethical and humane factors that need to be respected, but limit the margin of how the injury-induction can be carried out in the way it was done in this study. Uno de los actores clave en la regeneración de fibras musculares esqueléticas dañadas son las células satélite circundantes (células madre específicas), dispuestos entre la lámina basal y el sarcolema, en relativamente pocos números por fibra. Las células satélite, que son células precursoras miogénicas se activan durante lesión para fusionar y madurar en la fibra heridos. Algunos estudios han demostrado que el ejercicio y la hipoxia facilitan la activación y la migración en la circulación sanguínea, moviéndose más células a un área lesionada o activados que a su vez ayuda a la reparación de la lesión del tallo. El objetivo de esta tesis fue estudiar el perfil de regeneración más de catorce días después de una inducción excéntrico ejercicio de daño muscular esquelético en ratas de laboratorio caminadora entrenados, sometiéndolos a la serie de diario (4 h) la hipoxia hipobárica intermitente (con la altitud simulada de 4000 m), con o sin la combinación de ejercicio ligero, como parte de su rehabilitación. Las ratas podrían potencialmente reaccionan de manera diferente a la práctica de ejercicio, previa y la inducción de daño muscular, y además, ser diferente susceptible al protocolo de inducción de lesiones, influenciado por su formación. Esta premisa conducía a la creación de la puntuación de rendimiento AEY de las ratas a lo largo del estudio, como una herramienta complementaria para los datos adquiridos durante la regeneración de la lesión. La regeneración se perfila cuatro veces en los catorce días de recuperación de la inducción de lesiones a través de la concentración de mioglobina y la creatina quinasa (CK-MM) marcadores de plasma y análisis de la expresión diferencial de genes en m. sóleo, que generalmente se considera ser uno de el músculo más susceptibles a las lesiones excéntrica-ejercicio cuando se ejecuta cuesta abajo. Los resultados de los marcadores de plasma indican que el efecto del protocolo de la lesión de la inducción fue muy corta duración, ya que después de las muy diferentes concentraciones máximas para el control (recuperación pasiva) y la hipoxia grupos (sesiones de hipoxia intermitente durante la recuperación) después de la lesión de 1 día, había caído hasta el nivel basal de nuevo 3 días después de la lesión. Sin embargo, el grupo de hipoxia + ejercicio (sesiones de hipoxia intermitente seguido de ejercicio ligero durante la recuperación) tenía un perfil distinto con su pico lento aumento de 3 días después de la lesión y luego de descender de nuevo. Sin embargo, este perfil sólo fue significativa para las mediciones de CK-MM, mientras que la mioglobina no mostró ningún cambio significativo después de una lesión. Es evidente que las condiciones de hipoxia de ejercicio causan diferentes reacciones fisiológicas a la lesión. Sin embargo, el nivel de la lesión tendría que ser mayor con el fin de obtener un patrón más significativa y para ser capaz de interpretar si el patrón está reflejando efectos potencialmente beneficiosos o no. El análisis de la expresión diferencial de genes también indica que la inducción de lesiones necesario haber sido mayor para lograr la expresión diferencial. Los perfiles de la hipoxia y la hipoxia + ejercicio no son paralelas entre sí, siendo los datos también sugieren que las sesiones de hipoxia podría haber sido más eficaz. Por lo tanto, es difícil dar una conclusión general a la que la hipoxia o hipoxia + ejercicio facilitan la regeneración lesión a un grado superior. El hecho cierto es que hay factores éticos y humanos que necesitan ser respetado, pero limitan el margen de cómo la lesión de la inducción puede llevarse a cabo en la forma en que se llevó a cabo en este estudio.

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43

Ross, Shelley 1973. "Generation and characterization of mice lacking the α4 nicotinic receptor subunit". Monash University, Dept. of Medicine, 2001. http://arrow.monash.edu.au/hdl/1959.1/9180.

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Wong, Chun-wai. "The fate of undifferentiated murine embryonic stem cells in a mouse model with acute myocardial infarction." Click to view the E-thesis via HKUTO, 2005. http://sunzi.lib.hku.hk/hkuto/record/B31927634.

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Masters, Seth Lucian. "The role of the SPRY domain in the SPRY domain containing SOCS box proteins (SSBs) /." Connect to thesis, 2005. http://eprints.unimelb.edu.au/archive/00001571.

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Thesis (Ph.D.)--University of Melbourne, The Walter and Eliza Hall Institute of Medical Research, Division of Cancer and Haematology, Dept. of Medical Biology,Faculty of Medicine,Dentistry and Health Sciences, 2006. Typescript. Includes bibliographical references (leaves 192-210).

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46

Cheung, Kwok-ho Alvin. "Genetic and pharmacological approaches to study the role of the polyol pathway enzymes in diabetic and ischemic retinopathy." Click to view the E-thesis via HKUTO, 2007. http://sunzi.lib.hku.hk/HKUTO/record/B39558617.

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Yang, Fan. "Intervertebral disc regeneration using mesenchymal stem cells a mouse model study /." Click to view the E-thesis via HKUTO, 2007. http://sunzi.lib.hku.hk/HKUTO/record/B39556979.

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48

Tang, Wai-ho Jack. "Polyol pathway contributes to iron-induced oxidative damage in ischemia-reperfused rat hearts." Click to view the E-thesis via HKUTO, 2007. http://sunzi.lib.hku.hk/HKUTO/record/B39558022.

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49

曾漢文 and Hon-man Tsang. "Studies of Mll-Een fusion gene in a conditional mouse model of human leukemia." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2007. http://hub.hku.hk/bib/B39558034.

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Liu, Yan, and 劉艷. "The immunosuppressive effects of Triptolide and Rapamycin on mouse model of cardiac transplantation." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2007. http://hub.hku.hk/bib/B39793904.

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Tesis sobre el tema "Mice as laboratory animals"

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