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Artículos de revistas sobre el tema "Microsporea"

Autor: Grafiati

Publicado: 26 de julio de 2024

Última modificación: 31 de julio de 2025

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1

Devi Bunga Pagalla. "Stages of Microspore Development in Eggplant (Solanum melongena L.)." BIOEDUSCIENCE 7, no. 1 (2023): 68–72. http://dx.doi.org/10.22236/jbes/7111357.

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Background: Microspores are small haploid spores that develop into the male gametophyte. Microsporocytes undergo meiotic division to form microspores. Microspores can be found in seedless and seed plants. The microspores in each flowering plant are different. This study aims to observe microspores on eggplant flowers. Method: Microspore observations were carried out on different flower bud sizes until the flower buds bloomed. Result: The results showed microspores in eggplant had different stages of development for each flower bud size. The stages of microspore development observed were Early

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MANSOUR, Lamjed, Aouatef THABET, Abdel Halim HARRATH, et al. "New Microsporidia, Glugea sardinellensis n. sp. (Microsporea, Glugeida) Found in Sardinella aurita Valenciennes, 1847, Collected off Tunisian coasts." Acta Protozoologica 55, no. 4 (2016): 281–90. https://doi.org/10.4467/16890027AP.16.028.6097.

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MANSOUR, Lamjed, THABET, Aouatef, HARRATH, Abdel Halim, Y. AL OMAR, Suliman, MUKHTAR, Ahmed, R. SAYED, Shaban, ABDEL-BAKI, Abdel-Azeem Sh. (2016): New Microsporidia, Glugea sardinellensis n. sp. (Microsporea, Glugeida) Found in Sardinella aurita Valenciennes, 1847, Collected off Tunisian coasts. Acta Protozoologica 55 (4): 281-290, DOI: 10.4467/16890027AP.16.028.6097, URL: http://dx.doi.org/10.4467/16890027ap.16.028.6097

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3

Weidner, E., and A. Findley. "Extracellular Survival of an Intracellular Parasite (Spraguea lophii, Microsporea)." Biological Bulletin 197, no. 2 (1999): 270–71. http://dx.doi.org/10.2307/1542645.

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4

A.A., Atnagulova, and Ilyasova Z.Z. "STUDYING THE PREVALENCE OF ZOOANTHROPONOTIC MICROSPORIA AND DIAGNOSTIC METHODS." Russian Electronic Scientific Journal 50, no. 4 (2023): 421–33. https://doi.org/10.31563/2308-9644-2023-50-4-421-433.

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According to the study, children aged 3 to 10 years are more likely to suffer from microsporia. There were no significant differences in gender composition; the number of affected boys and girls was the same. In most cases, several family members are affected by microspores, i.e. Cases of infection predominate in families. The main source of microsporia infection was kittens, not adult animals. In most cases, parents sought medical help on time, but did not follow the rules for preventing fungal diseases - the source of infection was either left at home or thrown outside, which can infect othe

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5

Kisera, Y. V., Y. V. Martyniv та B. V. Gutyj. "Dynamics of morphological, immunological and histological changes in microsporіа in guinea pigs". Regulatory Mechanisms in Biosystems 12, № 2 (2021): 206–11. http://dx.doi.org/10.15421/022129.

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Microsporіа affect different species of animals and humans. The high contagiousness of the pathogen determines the relevance of research into this disease. Microsporum canis is the pathogen that most often causes microsporia. Weakened functions of the immune system and violation of the epithelial barrier of the skin are a favourable factor that causes microspores. The main source of infection is cats, which are involved in the storage and transmission of the pathogen. To clarify the dynamics of morphological, immunological and histological changes in microsporia, blood and skin studies of guin

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6

Pagalla, Devi Bunga, Ari Indrianto, Maryani Maryani, and Endang Semiarti. "Induction of Microspore Embryogenesis of Eggplant (Solanum melongena L.) ‘Gelatik’." Journal of Tropical Biodiversity and Biotechnology 5, no. 2 (2020): 124. http://dx.doi.org/10.22146/jtbb.53677.

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The haploid or double haploid plant of eggplants could be produced from microspore culture (embryogenesis of microspores). In the breeding programs, microspore can be developed into an embryo directly after exposure to stress treatment during cultured. Stress (temperature and starvation medium) is an important factor in the induction of embryogenesis microspore. This study aims to induced embryogenic microspores from eggplant CV. Gelatik. The stage late-uninucleate microspore (Vacuolate Microspore/VM) and early binucleate (Young Bicellular Pollen/YBP) are the suitable stages to induce multinuc

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7

Shaw, R. W., M. L. Kent, M. F. Docker, A. M. V. Brown, R. H. Devlin, and M. L. Adamson. "A New Species of Loma (Microsporea) in Shiner Perch (Cymatogaster aggregata)." Journal of Parasitology 83, no. 2 (1997): 296. http://dx.doi.org/10.2307/3284459.

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8

Lavrushko, S. I., and V. I. Stepanenko. "Modern diagnostics of microsporia." Ukrainian Journal of Dermatology, Venerology, Cosmetology, no. 2 (June 29, 2021): 16–24. http://dx.doi.org/10.30978/ujdvk2021-2-16.

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Objective — to develop a method of modern molecular genetic diagnosis of microsporia in children based on polymerase chain reaction (PCR), which will allow identification of the pathogen of Microsporum canis at the DNA level. Materials and methods. The study included 40 patients with microsporia of smooth skin, scalp, scalp and smooth skin. The biological materials for the research were scales from the smooth skin and scalp, hair from the scalp of patients with microsporia. A study of 40 samples of biological material was carried out in patients with microsporia of smooth skin, microspor

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9

Zhao, Z. Y., and D. F. Weber. "Analysis of Nondisjunction Induced by the R-X1 Deficiency during Microsporogenesis in Zea Mays L." Genetics 119, no. 4 (1988): 975–80. http://dx.doi.org/10.1093/genetics/119.4.975.

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Abstract The r-X1 deficiency in maize induces nondisjunction at the second mitotic division during embryo sac formation. However, it was not known if this deficiency also induces nondisjunction during the microspore divisions. Microsporogenesis in plants lacking or containing this deficiency was compared using two approaches. First, chromosome numbers were determined in generative nuclei. Many (8.3%) of the generative nuclei in r-X1-containing plants were aneuploid; however, those from control plants were all haploid. Thus, this deficiency induces nondisjunction during the first microspore div

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10

Nguyen, Minh Ly, and Ton Nu Bao Tien Huyen. "Effects of culture conditions on isolated microspore culture of melon (Cucumis melo L.)." Ministry of Science and Technology, Vietnam 65, no. 2 (2023): 30–36. http://dx.doi.org/10.31276/vjste.65(2).30-36.

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Isolated microspore culture is a useful tool to produce pure, fully homozygous parental lines in a short time. This study evaluated factors including the microspore developmental stage, cell culture density, and heat treatment influencing callus formation from melon microspores (Cucumis melo L.). The results showed that the obtained number of calli induced was highest (11.00±1.02) when cultivating flower buds with sizes 7.0-7.9 mm that contained microspores at middle-to-late uninucleate stages. The optimal microspore density for culture is4×104 cells. The cultured medium was NLN, containing 13

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11

Li, Zelin, Yuan Tang, Shengrong Chen, et al. "The correlation between the developmental stages of pea microspores and the morphology of flower buds." BIO Web of Conferences 142 (2024): 02008. https://doi.org/10.1051/bioconf/202414202008.

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To investigate the correlation between microspore development stage and the appearance and morphology of flower organs in Pisum sativum, the novel pea materials YP58 and YW90 were employed as experimental subjects. Flower buds exhibiting varying diametral ranges were chosen for cytological examination, and the saffron solid green staining method was employed to discern the diverse developmental stages and morphological attributes of microspores. Furthermore, the association between flower bud size and microspore development stage was examined. The findings revealed that the microspore developm

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12

Martyniv, Yu V., and Ia V. Kisera. "Changes of hematological parameters of in blood in cats ill with microsporium." Scientific Messenger of LNU of Veterinary Medicine and Biotechnology 21, no. 93 (2019): 70–73. http://dx.doi.org/10.32718/nvlvet9313.

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There has been a massive tendency for cats to be kept as pets in Ukraine in recent years. The frequency of their diseases has also increased at the same time. Cats most often come into the homes of people from the street, from volunteers, rarely from nurseries. Due to this, Doctors often receive cats ill for microsporia, which is caused by fungi of the genus Microsporum and is one of the most common anthropozoonous diseases. The treatment process is carried out by a complex method. Analysis of the recommendations of various authors on the treatment of microsporia indicates the lack of immunost

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13

Desser, Sherwin S., and John R. Barta. "Nosema jirivavrai n.sp. (Microsporea; Protozoa) from the leech Batracobdella picta in Ontario." Canadian Journal of Zoology 67, no. 11 (1989): 2640–45. http://dx.doi.org/10.1139/z89-373.

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A new microsporidian species was described from the muscle and connective tissue of the glossophoniid leech Batracobdella picta. Subepithelial xenomas contained sporonts, sporoblasts, and spores. Merogonic stages were not observed. All sporogonic stages were diplokaryotic. Mature spores were ovoid, 3.0–3.6 × 1.8–2.2 μm. The spore wall was 190–240 nm thick, with a distinct exospore, endospore, and underlying plasmalemma. The polar filament had 12–13 coils and measured 105 nm in diameter. The angle of tilt of the anterior coils of the filament to the vertical axis of the spore was about 63°. Thi

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14

Hause, G., B. Hause, and A. A. M. Van Lammeren. "Microtubular and actin filament configurations during microspore and pollen development in Brassica napus cv. Topas." Canadian Journal of Botany 70, no. 7 (1992): 1369–76. http://dx.doi.org/10.1139/b92-172.

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The structures of the microtubular and microfilamental cytoskeletons were investigated during the development of microspores and pollen grains of Brassica napus L. cv. Topas. Microfilaments were observed directly with rhodamine–phalloidin and microtubules with FITC by indirect immunofluorescent staining and transmission electron microscopy. We observed microtubules in all developmental stages and noted several changes in the configuration of the microtubular cytoskeleton during microspore development, microspore mitosis, and pollen development. A preprophase band before microspore mitosis was

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15

CRISTEA, Tina Oana. "The Influence of pH on Microspore Embryogenesis of White Cabbage (Brassica oleracea L.)." Notulae Scientia Biologicae 5, no. 4 (2013): 485–89. http://dx.doi.org/10.15835/nsb549122.

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In vitro microspore culture is one of the top techniques utilised now-a-days for the obtaining of double haploid plants in many plant species, including Brassica. The pH of the medium is a critical factor for the success of In vitro microspore culture as it influences the invertase enzyme activity, translated at cellular level through an acceleration or reduction of sucrose cleavage. The results published until now shows rather contradictory findings, as the response of microspores have been proved to be highly depending on genotypes, most of them being focused on Brassica napus. Thus, in the

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16

Pérez-Pérez, Yolanda, María Teresa Solís, Alfonso Albacete, and Pilar S. Testillano. "Opposite Auxin Dynamics Determine the Gametophytic and Embryogenic Fates of the Microspore." International Journal of Molecular Sciences 24, no. 13 (2023): 11177. http://dx.doi.org/10.3390/ijms241311177.

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The microspore can follow two different developmental pathways. In vivo microspores follow the gametophytic program to produce pollen grains. In vitro, isolated microspores can be reprogrammed by stress treatments and follow the embryogenic program, producing doubled-haploid embryos. In the present study, we analyzed the dynamics and role of endogenous auxin in microspore development during these two different scenarios, in Brassica napus. We analyzed auxin concentration, cellular accumulation, the expression of the TAA1 auxin biosynthesis gene, and the PIN1-like efflux carrier gene, as well a

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17

Indrianto, Ari, Chairani Siregar, Sutikno Linuhung, Mekartinita _, and Tri Sartikoningsih. "INDUCTION OF SPOROPHYTIC DIVISION IN ORCHIDS MICROSPORES BY STRESS." KnE Life Sciences 2, no. 1 (2015): 390. http://dx.doi.org/10.18502/kls.v2i1.181.

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<p>Orchid is one of the important ornamental plants in Indonesia this plant generally propagated by seed. Enhancing quality of this plant through breeding technology by various plant tissue culture methods and biotechnology, including doubled haploid technology are necessary. The most efficient method in creating doubled haploids plant is via microspore embryogenesis. We have develop new, innovative doubled haploid technology using the technique of isolated microspore culture. The goals are to obtain data on the male gametophyte development, viable embryogenic microspores, microspores de

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18

Földesiné Füredi, P., H. Ambrus, and B. Barnabás. "Development of cultured microspores of maize in the presence of n-butanol and 2-aminoethanol." Acta Agronomica Hungarica 60, no. 3 (2012): 183–89. http://dx.doi.org/10.1556/aagr.60.2012.3.1.

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The aim of the present study was to examine whether the induction of maize microspore embryogenesis could be triggered by the application of biogenic alcohols, as was reported earlier in wheat. A single cross hybrid (A 18) raised in the phytotron was used as anther donor for shed microspore cultures after cold pretreatment. At the onset of culturing, anthers in liquid YP medium were treated with 0.2 or 0.4% n-butanol or with 2 mM aminoethanol (2-AE) for 6 or 18 hours.The treatments caused a drastic (approx. 50%) decrease in the viability of the microspores. After a few days of culture in mediu

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19

Yao, Qing A., та Ken J. Kasha. "Potential off biolistic transformation of barley microspores based on viability and transient β-glucuronidase activity". Genome 40, № 5 (1997): 639–43. http://dx.doi.org/10.1139/g97-084.

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Microspores could be an excellent target for plant transformation, owing to their haploid nature, the availability of a large population of fairly synchronous single cells, and their potential to regenerate into plants through embryogenesis. Therefore, the potential for microspore transformation by biolistic procedures was examined cytologically, based on the viability and β-glucuronidase (GUS) activity of bombarded microspores. The microspores were bombarded with gold particles coated with the plasmid pAHC25. On average, 10.7% of the total number of microspores bombarded contained particles.

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Sumarmi, Sumarmi. "SELECTION OF SOYBEAN CULTIVARS: PREPARATION THE PLANT BREEDING WITH MICROSPORE CULTURE METHOD." Agric 30, no. 2 (2019): 125–33. http://dx.doi.org/10.24246/agric.2018.v30.i2.p125-133.

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Microspore culture method can be used as plant breeding program. The preparation of cultivars selection is an important step. The research starts with cultivated five cultivars of soybean i.e: Argomulyo, Grobogan, Wilis, Anjasmoro and Black Malika. The appearence of soybean plant was observed until flowering. Selection of plants based on: sum of flower bud every plant, anther midline, total and diameter of microspore every flower bud was measured by the ‘Optilab’ software. The development of microspore done with grouping of flower bud according long 2.02.5 mm, 2.6-3.0 mm, 3.1- 3.6 mm and 3.7-4

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Docker, MF, RH Devlin, J. Richard, J. Khattra, and ML Kent. "Sensitive and specific polymerase chain reaction assay for detection of Loma salmonae (Microsporea)." Diseases of Aquatic Organisms 29 (1997): 41–48. http://dx.doi.org/10.3354/dao029041.

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Kozar, Elena V., Elena G. Kozar, and Elena A. Domblides. "Effect of the Method of Microspore Isolation on the Efficiency of Isolated Microspore Culture In Vitro for Brassicaceae Family." Horticulturae 8, no. 10 (2022): 864. http://dx.doi.org/10.3390/horticulturae8100864.

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Isolated microspore culture in vitro (IMC) is an advanced technique for producing doubled haploids. We developed a modified microspore isolation method for the Brassicaceae family, which exceeds the results obtained by a standard microspore isolation method. We found that the new method allows an increase in the percentage of microspores at the embryogenic stage of development in the culture. In the spring rapeseed ‘Ratnik’ culture the percentage of microspores increases from 66.7% to 73%, and in the European radish ‘RBK’ from 34% to 61.9%. Moreover, the new method of microspore isolation made

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23

Austin, Publishing Group. "'FT', A Spontaneous Doubled Haploid Recipient of Transient Transformation in Chinese Cabbage." Annals of Agricultural & Crop Sciences 8, no. 2 (2023): 1132. https://doi.org/10.26420/annagriccropsci.2023.1132.

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Abstract Chinese cabbage is relatively recalcitrant to genetic transformation. Microspore is an ideal haploid single cell recipient for genetic m anipulation, whereby homozygous Doubled Haploid (DH) transformants can be obtained to accelerate the breeding process. In this study, Microspore Derived Embryo (MDE) productive material DH ‘FT’ is screened out from 10 candidates; microspore culture parameters were optimized: bud size ranged from 2.4mm to 2.7mm and microspore density 20000~40000/mL shows highest MDE yield. Antibiotic (chemical) sensitivity assay indicates that 100mg/L Carb

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Cristea, Tina Oana, Gabriel-Alin Iosob, Creola Brezeanu, and Petre Marian Brezeanu. "Effect of Chemical Composition of Nutritive Medium and Explant Size Over Androgenetic Response in Microspore Culture of Brassica oleracea L." Revista de Chimie 71, no. 10 (2020): 131–36. http://dx.doi.org/10.37358/rc.20.10.8357.

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The dimension of the bud is a key factor for the orientation of microspore culture and the success of obtaining double haploid plants as it is a strong correlation between bud size and the developmental stages of microspores, and it is specific for each plant species and genotype. Our study was focused to determine the correlation between morphological characteristics, namely floral bud size and specific microspore developmental stages in order to determine the proper size, suitable for a successful protocol of obtaining double haploid plants in Brassica oleracea var. italica. Thus, we tested

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Żur, Iwona, Ewa Dubas, Monika Krzewska, et al. "Triticale and barley microspore embryogenesis induction requires both reactive oxygen species generation and efficient system of antioxidative defence." Plant Cell, Tissue and Organ Culture (PCTOC) 145, no. 2 (2021): 347–66. http://dx.doi.org/10.1007/s11240-021-02012-7.

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AbstractThe effectiveness of microspore embryogenesis (ME) is determined by a complex network of internal and environmental factors. In the present study on triticale and barley, strong positive correlation (r = 0.85) between the generation of hydrogen peroxide (H2O2) and ME effectiveness confirmed the important role of reactive oxygen species in microspore reprogramming. However, for high effectiveness of ME induction, intensive H2O2 generation had to be associated with high activity of antioxidative enzymes, superoxide dismutase and catalase. The strong seasonal effect on the physiological s

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Shumilina, Daria, Dmitry Kornyukhin, Elena Domblides, Alexey Soldatenko, and Anna Artemyeva. "Effects of Genotype and Culture Conditions on Microspore Embryogenesis and Plant Regeneration in Brassica Rapa ssp. Rapa L." Plants 9, no. 2 (2020): 278. http://dx.doi.org/10.3390/plants9020278.

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Turnip is a biennial crop and, consequently, the creation of pure lines for breeding is a time-consuming process. The production of pure turnip lines using doubled haploids produced in isolated microspore culture has not been sufficiently developed. The aim of the present work was to determine some key factors inducing embryogenesis in the isolated microspore culture of turnip, as well as investigating the manners of embryo development. It was shown that the acidity of the medium is an important factor in embryo production; different optimal pH levels ranging from 6.2 to 6.6 corresponded to in

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Kisera, Ia V., and Yu V. Martyniv. "The selection of the concentration of clotrimazole and povidone-iodineas the main active substances of the “Micromar” antifungal agent." Scientific Messenger of LNU of Veterinary Medicine and Biotechnologies 21, no. 95 (2019): 27–31. http://dx.doi.org/10.32718/nvlvet9505.

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Microsporia is one of the most common skin diseases of cats, most often provoked by the pathogen Microsporum canis. The pathogen of microsporium has highly contagious properties and for a long time remains capable of infection in the environment. It is important for the veterinary doctor not only to carry out complex therapy, but also to prevent the spread of the pathogen in the environment and to prevent the occurrence of secondary pyoderma during the course of microspores. Due to the tendency to the growth of fungal diseases, the development of the pathogen’s resistance existing medicines, t

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Rodrigues, Lia Rosane, Bianca de Camargo Forte, and Maria Helena Bodanese-Zanettini. "Isolation and culture of soybean (Glycine max L. Merrill) microspores and pollen grains." Brazilian Archives of Biology and Technology 49, no. 4 (2006): 537–45. http://dx.doi.org/10.1590/s1516-89132006000500002.

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In the last three decades, research on soybean microspore embryogenesis was restricted to anther culture, which presents limitations such as the small number of responsive microspores and the high embryogenic potential of sporophytic tissues. Therefore, a sequence of studies was performed to establish appropriate conditions for the isolation and culture of soybean microspores and pollen grains as an alternative to anther culture. First, a pollen and microspore isolation technique was developed using floral buds from four soybean cultivars (Bragg, IAS 5, MG/BR-46 Conquista and BRSMT Uirapuru).

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Djatchouk, T. I., O. V. Khomyakova, V. N. Akinina, I. A. Kibkalo, and A. V. Pominov. "Microspore embryogenesis in vitro: the role of stresses." Vavilov Journal of Genetics and Breeding 23, no. 1 (2019): 86–94. http://dx.doi.org/10.18699/vj19.466.

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Gametic embryogenesis is one form of totipotency of plant cells, in which either male or female gametes are induced to form embryoids (sporophytes). Regeneration of haploid plants from embryoids and subsequent chromosome duplication result in doubled haploids and DH-lines. The production of haploids and doubled haploids (DHs) through gametic embryogenesis allows a single-stage development of complete homozygous lines from heterozygous plants. The development of effective haploid protocols to produce homozygous plants has a significant impact on plant breeding, shorting the time and costs requi

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Charzyńska, Maria, and Joanna Maleszka. "3H-thymidyne incorporation into the microspores and pollen grains nuclei in excised Tradescantia stamens." Acta Societatis Botanicorum Poloniae 47, no. 1–2 (2015): 163–72. http://dx.doi.org/10.5586/asbp.1978.014.

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The development of microspores and pollen grains lasts in <i>Tradescantia bracteata in vivo</i> from the tetrad stage to pollen shedding about 14 days. This including 7 days of the microspore life cycle. In stamens excised and placed on a medium the microspores and pollen grains develop normally for at least 3 days. <sup>3</sup>H-thymidine is added into medium culture. DNA synthesis m the microspore nucleus is demonstrated 6 days after tetrad formation so at the end of microspore interphase. During synthesis the nucleus lies at one end of the long axis of the vacuolated

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Wei, Dongmei, Huimin Xu, and Ruili Li. "Dynamics of Polysaccharides and Neutral Lipids during Anther Development in Castor (Ricinus communis)." Journal of the American Society for Horticultural Science 140, no. 4 (2015): 356–61. http://dx.doi.org/10.21273/jashs.140.4.356.

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Anthers contain starch and neutral lipids, which have key roles in microspore ontogeny and gametophyte development. In this study, we observed the dynamic changes in starch and neutral lipids in the anther developmental processes of castor (Ricinus communis) by cytochemical methods. Starch grains and neutral lipids presented a regular dynamic distribution during anther development. In young anthers, some neutral lipids accumulated in sporogenous cells, whereas neutral lipids disappeared with microspore growth. At the late microspore stage, starch grains began to accumulate in microspores, and

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Picard-Sánchez, Amparo, M. Carla Piazzon, Itziar Estensoro, et al. "Experimental Horizontal Transmission of Enterospora nucleophila (Microsporea: Enterocytozoonidae) in Gilthead Sea Bream (Sparus aurata)." Animals 11, no. 2 (2021): 362. http://dx.doi.org/10.3390/ani11020362.

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Enterospora nucleophila is a microsporidian enteroparasite that infects mainly the intestine of gilthead sea bream (Sparus aurata), leading to an emaciative syndrome. Thus far, the only available information about this infection comes from natural outbreaks in farmed fish. The aim of the present study was to determine whether E. nucleophila could be transmitted horizontally using naturally infected fish as donors, and to establish an experimental in vivo procedure to study this host–parasite model without depending on natural infections. Naïve fish were exposed to the infection by cohabitation

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Giełwanowska, Irena, Anna Bochenek, and Ewa Szczuka. "Development of the pollen in the antarctic flowering plant Colobanthus quitensis (Kunth) Bartl." Acta Agrobotanica 60, no. 2 (2012): 3–8. http://dx.doi.org/10.5586/aa.2007.023.

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<i>Colobanthus quitensis</i> (Kunth) Bartl. produced two types very small bisexual fl owers. In the Antarctic natural conditions chasmogamic and cleistogamic fl owers most often form fi ve stamina with short fi laments. Two microsporangia with a three-layer wall form in the anther. Microspore mother cells, which develop into microspores after meiosis, form inside the microsporangium. Microsporocytes of <i>Colobanthus quitensis</i> are surrounded with a thick callose layer, the special wall. After meiosis, the callose wall is dissolved and microspores are released from t

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Fuchs, K., and K. P. Pauls. "Flow cytometric characterization of microspore development in Brassica napus." Canadian Journal of Botany 70, no. 4 (1992): 802–9. http://dx.doi.org/10.1139/b92-102.

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Flow cytometry was found to be sensitive to change in the physical characteristics of developing Brassica napus microspores. By measuring forward angle (10° – 19°) light scatter (FALS) and log 90° light scatter (L90LS) several stages of microspore development were characterized in small (1.5 mm to 2.5 mm), medium (2.5 mm to 3.5 mm), and large (3.5 mm to 4.5 mm) buds. Cell sorting was used to identify the types of cells that made up the subpopulations in two parameter plots of FALS versus L90LS including tetrad, translucent, trilobate, round, and oval microspores. Microspore development was fou

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35

Bakos, F., A. Fábián, and B. Barnabás. "Isolated microspore cultures of a Hungarian durum wheat ( Triticum turgidum L.) cultivar, Martondur 1." Acta Agronomica Hungarica 55, no. 2 (2007): 157–64. http://dx.doi.org/10.1556/aagr.55.2007.2.3.

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A number of sporophytically induced microspores and embryo-like structures (ELS) were obtained from isolated microspore cultures of durum wheat ( Triticum turgidum L. cv. Martondur 1). Various pre-treatments were screened, involving spike treatment at 4°C for 2, 7 or 14 days; anther treatment in 0.4 M mannitol containing macroelements at 33°C for 3 days, and various combinations of these. The frequency of embryogenic (star-like) microspores and the number of ELS showed a very high positive correlation in the cultures. Starvation at high temperature was necessary to achieve a reasonable frequen

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36

Kurmann, Marie H. "Pollen wall formation in Abies concolor and a discussion on wall layer homologies." Canadian Journal of Botany 67, no. 8 (1989): 2489–504. http://dx.doi.org/10.1139/b89-319.

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Development of the pollen wall in Abies concolor (Gord. & Glen.) Lindl. is documented using ultrastructural techniques. Pollen wall formation is initiated by the deposition of a microspore surface coat and the formation of callosic protrusions in the proximal and lateral areas, outside the plasma membrane of the microspores enclosed in tetrads. The alveolar ektexine is then elaborated on these structures. Endexine formation starts with the appearance of tripartite lamellae to the inside of the microspore surface coat around the entire microspore. Both the ektexine and endexine are elaborat

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37

Bal, Ugur, Sebnem Ellialtioglu, and Kazim Abak. "Induction of symmetrical nucleus division and multi-nucleate structures in microspores of eggplant (Solanum melongena L.) cultured in vitro." Scientia Agricola 66, no. 4 (2009): 535–39. http://dx.doi.org/10.1590/s0103-90162009000400016.

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A modification of a protocol used to induce tobacco microspore embryogenesis was tested in eggplant (Solanum melongena L.). In tobacco, uninucleate microspores are subjected to stress treatment by culturing in mannitol containing "B" medium at 33ºC for six days. The microspores are then transferred to maltose containing AT3 medium for further development. In the experiment presented here late uninucleate and bi-nucleate microspores of the eggplant cultivar Bambino were pre-cultured in B medium and then incubated at +4ºC, 25ºC and 33ºC, respectively, for two days. After the pre-treatments, micr

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38

Gao, Yue, Gaoyang Qu, Shengnan Huang, et al. "Comparison between Germinated Seed and Isolated Microspore EMS Mutagenesis in Chinese Cabbage (Brassica rapa L. ssp. pekinensis)." Horticulturae 8, no. 3 (2022): 232. http://dx.doi.org/10.3390/horticulturae8030232.

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Mutagenesis is an important tool for breeding and genomic research. In this study, the germinated seeds and isolated microspores of a double haploid line ‘FT’ were treated with EMS, respectively, with the aim of comparing the effects of the two approaches on generating mutants in Chinese cabbage. For microspore EMS mutagenesis, the isolated microspores were treated with 0.12% EMS for 20 min, a total of 1268 plantlets were obtained, and 15 M1 mutants were screened with a mutation frequency of 1.2%. For seed EMS mutagenesis, 7800 germinated seeds were treated with 0.8% EMS for 12 h, and a total

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39

Hu, Tianci, and Ken J. Kasha. "A cytological study of pretreatments used to improve isolated microspore cultures of wheat (Triticum aestivum L.) cv. Chris." Genome 42, no. 3 (1999): 432–41. http://dx.doi.org/10.1139/g99-002.

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Isolated microspores of wheat can be induced in vitro to switch their development from the gametophytic pollen pathway to a sporophytic pathway, resulting in embryoid or callus formation. The influence of cold or mannitol pretreatment on karyokinesis and cytokinesis in isolated microspore culture responses were investigated. Anthers were pretreated in mannitol for 7 d at 28°C; spikes at 4°C for 28 d. Microspores often completed the 1st mitotic nuclear division during pretreatment while cytokinesis was delayed. During mannitol pretreatments, the 1st mitotic nuclear division was mostly symmetric

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40

Dilcher, D. L., R. K. Kar, and M. E. Dettmann. "The functional biology of Devonian spores with bifurcate processes-a hypothesis." Journal of Palaeosciences 41 (December 31, 1992): 67–74. http://dx.doi.org/10.54991/jop.1992.1107.

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Aquatic heterosporous ferns may have grapnel like glochidia, e.g., Azolla, specialized for anchoring a microspore mass to a megaspore. Thus, in an aquatic system, the free floating microspore mass (glochidia) and megaspore are held in close proximity when the sperm cells are released. Similar structures are known from the Cretaceous and Tertiary megaspores and microspores such as Azollopsis and Ariadnaesporites and are considered to have functioned in fertilization. This demonstrate that, as part of the evolution of the aquatic heterosporous habit in the ferns during the Cretaceous, functional

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41

Rowley, John R., John J. Skvarla, and Gamal El-Ghazaly. "Transfer of material through the microspore exine from the loculus into the cytoplasm." Canadian Journal of Botany 81, no. 11 (2003): 1070–82. http://dx.doi.org/10.1139/b03-095.

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Our results and those we review indicate that the exine has a great capacity for modifications that enable nutrients to pass through from the anther loculus to the microspore cytoplasm. Avenues of passage include strands, some of which are viscin threads, from the tapetum to microspores in, for example, Betula, Fuchsia, and Epilobium. Micro channels in Lopezia, Gaura, and Gelsemium extend through the ectexine, endexine, and intine to the cytoplasm. The bulge regions in Epilobium represent portions of the endexine that become very greatly enlarged, forming conducting channels that transport mat

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42

Lavrushko, S. I., and V. I. Stepanenko. "Research of efficiency of microsporia diagnostics methods." Ukrainian Journal of Dermatology, Venerology, Cosmetology, no. 3 (October 1, 2021): 21–26. http://dx.doi.org/10.30978/ujdvk2021-3-21.

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Objective — to investigate the effectiveness of different methods of microsporia diagnostics in children. Materials and methods. 50 children aged 2 to 16 (24 boys and 26 girls) were under survey. Depending on the clinical course, diagnosis and research results, all patients were divided into two groups: the 1st group included 40 children with microsporia (19 — with smooth skin microsporia, 13 — with scalp microsporia, 8 — with scalp and smooth skin microsporia); and the 2nd group consisted of 10 children in whom microsporia was not detected. The clinical diagnosis of all patients of the

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43

Herd, Y. R., E. G. Cutter, and I. Watanabe. "A light and electron microscopic study of microsporogenesis in Azolla microphylla." Proceedings of the Royal Society of Edinburgh. Section B. Biological Sciences 86 (1985): 53–58. http://dx.doi.org/10.1017/s0269727000007958.

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SynopsisMicrosporogenesis in cultured material of Azolla microphylla was studied with the light and transmission electron microscopes. The first formed sporangium, a megasporangium, aborts and several microsporangia develop below. Initially, a single sporogenous cell is present, surrounded by a single layered tapetum and the microsporangial wall. Subsequently, several sporogenous cells are connected by plasmodesmata. The microspore mother cells are less densely cytoplasmic than the tapetal cells. Callose-like material is deposited around the microspore mother cells, but disappears before meios

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44

Polowick, P. L., and V. K. Sawhney. "An ultrastructural study of pollen development in tomato (Lycopersicon esculentum). I. Tetrad to early binucleate microspore stage." Canadian Journal of Botany 71, no. 8 (1993): 1039–47. http://dx.doi.org/10.1139/b93-120.

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Microspores undergo considerable ultrastructural changes between the tetrad and early binucleate microspore stages of microsporogenesis in tomato (Lycopersicon esculentum). Pollen wall deposition began late in the tetrad stage, and by the early microspore stage a lamellar foot layer and tectum were deposited. Sculpturing of the tectum was evident by the early binucleate microspore stage. Dictyosomes and vesicles were abundant during the period of pollen wall formation. Plastids were associated with the endoplasmic reticulum (ER) to form plastid–ER complexes, from the late tetrad to the vacuola

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45

Berenguer, Eduardo, María-Teresa Solís, Yolanda Pérez-Pérez, and Pilar S. Testillano. "Proteases with caspase 3-like activity participate in cell death during stress-induced microspore embryogenesis of Brassica napus." EuroBiotech Journal 3, no. 3 (2019): 152–59. http://dx.doi.org/10.2478/ebtj-2019-0018.

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Abstract Microspore embryogenesis is a model system of plant cell reprogramming, totipotency acquisition, stress response and embryogenesis initiation. This in vitro system constitutes an important biotechnological tool for haploid and doubled-haploid plant production, very useful for crop breeding. In this process, microspores (cells that produce pollen grains in planta) are reprogrammed toward embryogenesis by specific stress treatment, but many microspores die after the stress. The occurrence of cell death is a serious limiting problem that greatly reduces microspore embryogenesis yield. In

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46

Huang, Bin. "Genetic manipulation of microspores and microspore-derived embryos." In Vitro Cellular & Developmental Biology - Plant 28, no. 2 (1992): 53–58. http://dx.doi.org/10.1007/bf02823018.

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47

Han, Yang, Xue Ling Ye, and Hui Feng. "Improved Efficiency of Microspore Culture of Brassica campestris Ssp. pekinensis (Chinese Cabbage)." Applied Mechanics and Materials 675-677 (October 2014): 1091–96. http://dx.doi.org/10.4028/www.scientific.net/amm.675-677.1091.

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[Objective](1) to investigate the factors that influence microspore embryogenesis and plantlet regeneration;(2) to discuss some protocols for the induction culture of microspore-derived embryos and for rooting and transplantation of microspore-derived plantlets. [Proposed Methods] B. campestris ssp. pekinensis ‘Futian 50’ and B. campestris ssp. pekinensis ‘Changkuai’ were used as the experimental materials, then their microspores were cultured in NLN media. [Results] NAA and 2,4-D inhibits the formation of microspore-derived embryos. Low concentrations of cytokinins facilitate embryogenesis, w

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48

Lauxen, Mozart da Silva, Eliane Kaltchuk- Santos, Ching yeh Hu, Sidia Maria Callegari- Jacques, and Maria Helena Bodanese-Zanettini. "Association between floral bud size and developmental stage in soybean microspores." Brazilian Archives of Biology and Technology 46, no. 4 (2003): 515–20. http://dx.doi.org/10.1590/s1516-89132003000400004.

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This study was carried out to establish the association between floral bud size and the corresponding microspore developmental stages for Brazilian soybean cultivars. Microspore developmental stage distributions were examined in young buds from cv Década, IAS5 and RS7. The data indicated that for a given bud-size group, the microspores of different cultivars were at different developmental stages, with cv RS7 and Década distributed at the youngest and cv IAS5 at the most advanced stages. Microspore stages distribution were also compared among the ten anthers of the same bud of the above cultiv

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49

Ziauddin, Asma, Mingsheng Peng, and David J. Wolyn. "Improved Nuclear Staining of Asparagus Microspores for Cytological Analysis." HortScience 32, no. 4 (1997): 735–36. http://dx.doi.org/10.21273/hortsci.32.4.735.

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Clear visualization of asparagus (Asparagus officinalis L.) microspore nuclei with common stains such as acetocarmine or DAPI is difficult, hindering cytological analyses. The addition of saturated aqueous ferric chloride solution to Carnoy's I fixative (30 μL·mL-1) resulted in clear visualization of nuclei. A distinct nucleus was observed in uninucleate cells and the vegetative and generative nuclei were clearly visible in binucleate microspores. This method can be used reliably for determination of asparagus microspore developmental stage. Chemical name used: 4′,6-diamidino-2-phenylindole-2H

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50

Kent, ML, and SC Dawe. "Efficacy of Fumagillin DCH against experimentally induced Loma salmonae (Microsporea) infections in chinook salmon Oncorhynchus tshawytscha." Diseases of Aquatic Organisms 20 (1995): 231–33. http://dx.doi.org/10.3354/dao020231.

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