Literatura académica sobre el tema "Mini-cuttings"

The mini-cuttings productivity in clonal mini-garden using the semi-hydroponic system is a promising technique for the olive propagation, to obtaining a satisfactory amount of cuttings and with good quality. The objective was to evaluate the mini-stumps productivity and rooting of olive mini-cuttings in successive collections at different times of year in a clonal mini-garden in a semi-hydroponic system. In each collection the productivity of the clonal mini-garden was evaluated counting the collected cuttings. The mini-cuttings were prepared with 3-5 cm long with leaf area reduced by 50% and placed in plastic boxes with vermiculite for a period of 80 days. The evaluated variables were: mini-stumps productivity, mini-stumps and mini-cuttings percentage of survival, rooting percentage and number and length of mini-cuttings root. The olive mini-cuttings presented higher productivity during the spring of 2014. The mini-cuttings rooting percentage collected during the evaluated periods demonstrated that mini-cutting is a viable technique for olive propagation when grown in clonal mini-garden using a semi-hydroponic system.

ABSTRACT This study presents an efficient system with high productivity and quality for Acer palmatum propagation. We evaluated the efficiency of mini-cuttings technique, management of mini-stumps, production of shoots, rooting and root vigor of mini-cuttings on two seasons. The mini-stumps were planted in pots of two liters, put up in two environments (full sunlight area and shade house) and submitted to weekly fertigation. Over six collections, we evaluated the survival and productivity of clonal mini-garden (Experiment I). From these, we performed two experiments with mini-cuttings: Experiment II - mini-cuttings originated from two environments, with 8±1 cm, put to root in two seasons (Spring and Summer); Experiment III - mini-cuttings originated from full sunlight area, with 4±1, 6±1, 8±1, 10±1 cm, put to root in Summer. The mini-cuttings planting was made in plastic tubes of 55 cm³, filled with fine vermiculite and carbonized rice hulls (1:1 v/v), in a greenhouse with intermittent irrigation system. The high rooting (95.0%), mini-stumps survival (100%) and mini-cuttings production (217.5 mini-cuttings m-2 month-1) in clonal mini-garden conducted in full sunlight area shown the technique viability for the species. Mini-cuttings of 8±1 cm favored rooting (96.3%). The mini-cuttings technique is viable for Acer palmatum propagation.

Abstract: The objective of this work was to define an early selection strategy to identify Cabralea canjerana (Meliaceae) clones with high multiplication rate. A mini-garden of 109 clones of canjerana seedlings was established in a completely randomized design, in an acclimatized greenhouse. From seedlings, the mini-stumps and mini-cuttings were obtained. Mini-cuttings were collected at five different times, and the number of mini-cuttings per mini-stump, rooting percentage, and number of rooted mini-cuttings were quantified. The number of rooted mini-cuttings per mini-stump was the only trait that showed high correlation with the others. Five groups of clones based on the number of rooted mini-cuttings per mini-stump were separated using k-means clustering, and the genetic gain from selection and Pearson correlation were estimated. The selection of the two best groups in each evaluation period resulted in high genetic gains from selection for all evaluated traits. Early selection for the number of rooted mini-cuttings discarded 65% of the evaluated clones, which increases experimental precision in evaluations of traits associated with plantlet growth and quality. Early selection for the number of rooted mini-cuttings per mini-stump at different times allows the identification of Cabralea canjerana clones with high multiplication rate.

ABSTRACTWe aimed to evaluate the technical efficiency of mini-cuttings technique on vegetative propagation of Paulownia fortunei (Seem.) Hemsl. var. Mikado, as well as the possible existence of anatomical barriers to its rooting. Therefore, plants originated from cuttings formed the mini-stumps and, consequently the clonal mini-garden, which was conducted in semi-hydroponic system. We evaluated the survival of mini-stumps and sprouts production for five successive collects, the percentage of mini-cuttings rooting and their anatomical description. The mini-cuttings were prepared with about 6 to 8 cm in length and two leaves reduced by about 50% in the upper third, being remained an area of, approximately 78 cm2 (10 cm diameter). The mini-cuttings were placed in tubes of 53 cm3, with substrate formed with fine vermiculite and carbonized rice hulls (1:1 v/v) and rooted in acclimatized greenhouse. After 30 days we evaluated the percentage of rooted mini-cuttings, radicial vigor (number and length of roots / mini-cutting), callus formation, emission of new shoots and maintenance of the original leaves. The mini-stumps showed 100% survival after five collects and an average production of 76-114 mini-cuttings/m2/month and rooting ranged from 70 to 90%. Mini-cuttings technique is efficient in to propagate adult propagules of the species and there are not anatomical barriers preventing roots emission.

This study aimed to evaluate the production of cordia (Cordia trichotoma) ministumps of seminal and asexual origin, as well as the effect of the naphthaleneacetic acid (NAA) on the rooting of mini-cutitings. Seedlings and plantlets from rooted cuttings were pruned to form a clonal mini-hedge. The ministumps were fertirrigated with three concentrations of nutrient solution and they were evaluated for survival and production of mini-cuttings. The mini-cuttings were or not treated with 1000mg L-1 of NAA and planted in substrate for rooting. The mini-cuttings were evaluated for the percentages of survival and rooting, and number and total length of roots after 30 days of cultivation. There was no difference in survival and number of mini-cuttings obtained from seminal or asexual ministumps. Mini-cuttings from ministumps of asexual origin showed higher rooting capability. The use of 1000mg L-1 NAA did not increase the rooting percentage of cordia mini-cuttings.

With the need of a large-scale seedling production, the application of purple nutsedge extract may represent a promising alternative, contributing to the fruit species propagation. The aim of this study was to evaluate the effects of nutsedge extract on the survival rate, rooting and biomass of acerola’s mini-cuttings. The experimental design was a completely randomized block, with a 5x2 factorial arrangement (five aqueous purple nutsedge extracts of nutsedge (0%, 25%, 50%, 75% and 100%) and two types of mini-cuttings, a pair of entire leaves and a pair of half leaves) resulting in 10 treatments with 5 repetitions and 8 mini-cuttings per repetition. The evaluations were performed at 75 days after planting, when destructive analysis were carried out for morphological characteristics. The evaluates variables were mini-cuttings survival percentage, percentage of mini-cuttings with sprouts, percentage of mini-cuttings with callus, percentage of minicuttings with roots, root system length, shoot dry mass, root dry mass, total dry biomass and the ratio between shoot and root dry mass. For the experiment conditions, the nutsedge extract positively influenced the survival percentage and percentage of sprouting of acerola’s mini-cuttings, however the extract application did not influence the mini-cuttings biomass of, being the results observed with or without the cut on mini-cuttings leaves.

Drimys brasiliensis, popularly known as cataia or casca-d'anta, is a tree species native to the Atlantic Forest and belonging to the family Winteraceae. Due to its seeds have dormancy due to embryonic immaturity, the present work aimed to evaluate the viability of the mini-cutting technique in D. brasiliensis in different seasons of collection of the propagules. Mini-cuttings from seedlings sprouts previously produced by conventional cutting were collected at six different times (December/2015, February/2016, April/2016, June/2016, August/2016 and October/2016), made 6-8 cm long, with bevel cut at the base and rectum at the apex, keeping two leaves with their area halved. The experiment was carried out in a completely randomized design with 4 replicates and 14 mini-cuttings per experimental unit. The mini-cuttings were planted in tubes with substrate containing vermiculite and carbonized rice husk (1:1) and kept in a heated greenhouse. After 120 days, the percentage of rooted mini-cuttings, number of roots per mini-cuttings, length of the three largest roots by mini-cuttings, percentage of mini-cuttings with callus, live, dead, with shoots and leaves that maintained their original leaves were evaluated. Rooting percentages of mini-cuttings were greater than 75% regardless of the season of the year in which the propagules were collected. The adventitious root formation of D. brasiliensis occurs indirectly, from the callus tissue formed at the base of the mini-cuttings. The mini-cuttings technique is recommended for the production of seedlings of the species, at any seasons of the year.

ABSTRACT The Australian species Melaleuca alternifolia Cheel. has a strong commercial importance due to the extraction of essential oils from its leaves used in the cosmetic and pharmaceutical industry. In order to obtain an efficient plant production system of M. alternifolia the mini-cuttings technique and the clonal mini-garden management in the productivity and rooting of mini-cuttings and different substrate compositions were analyzed during all the seasons. Mini-stumps derived from cuttings and grown in pots (2 L), were submitted to successive harvesting of their sprouts during the four seasons (september/2013 to august/2014). From the mini-stumps sprouts mini-cuttings were produced, wich were were planted in plastic tubes and kept in a greenhouse for 45 days. Six substrates were used for planting the mini-cuttings: commercial substrate (S1); substrate composed of 100% carbonized rice husk (CRH) (S2); substrate composed of 100% coconut fiber (CF) (S3); substrate composed of 50% CF and 50% CRH (S4); substrate composed of 30% CF and 70% CRH (S5); substrate composed of 70% FC and 30% CRH (S6). The high survival of mini-stumps (over 90%) and the mini-cuttings production (282 mini-cuttings.m-2.month-1) in the shade house demonstrate the technical feasibility for the species, being summer the most appropriate time to collect propagules. The substrate composed by 70% CF + 30% CRH (S6) shows superior results for vegetative propagation of M. alternifolia (91.7% of rooted mini-cuttings), as the single one to contemplate simultaneously all parameters. Summer is recommended as the best time for rooting of mini-cuttings.

ABSTRACT: This study aimed to evaluate the rooting capability of apical and nodal mini-cuttings of ipe-roxo ( Handroanthus heptaphyllus ) in different substrates. Shoots produced from mini-stumps were fractioned into mini-cuttings 2-cm long apical or nodal mini-cuttings and treated with 1000 mg L-1 of indolebutyric acid (IBA). Four substrate combinations were evaluated: commercial substrate composed of pine bark and vermiculite in proportions of 1:1, 2:1 and 1:2 (v /v) and commercial substrate, vermiculite and sand in the proportion of 1:1:1 (v/v). The factorial experiment utilized a completely random design, with five replicates of four mini-cuttings. Rooting, shooting and survival percentage, number and length of shoots and roots were evaluated at 60 and 90 days of growth. Commercial substrate and vermiculite are a good combination for rooting mini-cuttings of ipe-roxo. Apical and nodal mini-cuttings exhibit similar rooting behavior and both are feasible for production of ipe- roxo plantlets.

AbstractIlex paraguariensis, commonly known as yerba mate, is a tree species native to South America. Its commercial value is due to the manufacturing of teas, with potential also in the pharmacological and cosmetic industries. Vegetative propagation of yerba mate is considered an innovation to the traditional production systems based on sexual propagation. The present study aimed to evaluate the rhizogenic potential and chemical attributes of mini-cuttings from 15 yerba mate genotypes, as well as to verify the correlation between phytochemical and rooting-related variables. Mini-cuttings were collected from a pre-existing mini-clonal hedge and the experimental design was completely randomized, with 15 treatments (genotypes), four replications and 10 mini-cuttings per plot. After 120 days, mini-cuttings were assessed regarding rooting, mortality, callogenesis and leaf retention percentages, percentage of mini-cuttings with both calluses and roots, number of roots and average root length. At the time of collection, subsamples from each plot were used for phytochemical analyses including total phenolic compounds, protein, caffeine and theobromine contents and antioxidant activity. Rooting percentages ranged from 5 to 72.5%, with significant variation among genotypes. Adventitious rooting and phytochemical profile of yerba mate mini-cuttings are genotype-dependent. Leaf retention is a relevant factor in the rooting of yerba mate mini-cuttings and the levels of total phenolic compounds, antioxidants and theobromine present in mini-cuttings are negatively correlated components to Ilex paraguariensis adventitious rooting.

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Micro-propagation is a common technique to produce disease free potato materials. Mini-cuttings of just acclimatized plantlets or older plants associated with micro-propagation may reduce production costs of potato mini-tubers. This work was carried out to study the micro-propagation and mini-cutting techniques to maximize the production of mini-tubers and to minimize the costs of potato seeds. Two experiments were conducted, being in the first evaluated the micro-propagation and acclimatization of plantlets and in the second evaluated the mini-cutting of potato plants. The first experiment was conducted in two steps. In vitro growth of potato explants were evaluated in two concentrations of MS medium (½ MS and MS), three sucrose doses (30, 45 and 60 g L-1), and three clones (Asterix, Macaca e SMINIA793101-3). The same treatment combinations were evaluated during plantlet acclimatization in two systems (floating and sand). The mini-cutting was evaluated in three experiments. The effect of irrigation solution (tap water, nutrient solution and the combination) and the physiological age of stock plant (just acclimatized and in mini-tuber production) were evaluated in the mini-cutting rooting of Asterix, Macaca and SMINIA793101-3 clones. A third experiment was carried out to evaluate the effect of indol butyric acid (IBA) (0; 300; 600; 900 ppm) in mini-cutting rooting of Asterix clone. The MS medium with 50% of the salt concentration increases the plantlet survival during acclimatization. The sucrose has no effect on plantlet acclimatization. Both floating and substrate (sand) are suitable systems for acclimatization of potato plantlets. Mini-cuttings root in sand as substrate irrigated with tap water or nutrient solution. Apical mini-cuttings are suitable for the production of potato plantlets. Rooting potential of mini-cuttings is affected by the advance of the physiological age of the stock plant.
A micropropagação é uma técnica comumente utilizada para a produção de material propagativo de batata livre de doenças. Associada a essa técnica, a miniestaquia de plântulas recém aclimatizadas ou em produção pode ser uma forma de reduzir os custos de produção de minitubérculos de batata. Este trabalho foi conduzido com o objetivo de estudar a micropropagação e a miniestaquia, para maximizar a produção de mudas e minimizar os custos da batata-semente. Dois experimentos foram conduzidos, sendo no primeiro avaliado a micropropagação e aclimatização de plântulas e, no segundo, avaliado a miniestaquia de plantas batata. O primeiro experimento foi conduzido em duas etapas. Em laboratório foi avaliado o crescimento dos explantes em função da combinação de concentrações do meio MS (½ MS e MS completo), com doses de sacarose (30, 45 e 60 g L-1) em três clones (Asterix, Macaca e SMINIA793101-3). Em telado foi avaliado o efeito dos tratamentos aplicados na micropropagação sobre a aclimatização de plântulas em dois sistemas (flutuação e areia). A miniestaquia foi estudada em três experimentos. Um experimento foi conduzido para avaliar o efeito da solução de irrigação (água, solução nutritiva e a combinação de ambas) e outro a idade da planta matriz (recém aclimatizada e em produção) sobre o enraizamento de miniestacas dos clones Asterix, Macaca e SMINIA793101-3. Um terceiro experimento foi conduzido para avaliar o efeito do ácido indol-butírico (AIB) (0, 300, 600 e 900 mg L-1) no enraizamento das miniestacas do clone Asterix. O meio ½ MS, independente da dose de sacarose, aumenta a sobrevivência das plântulas de batata durante a aclimatização. Tanto o sistema com flutuação, quanto com substrato (areia) podem ser utilizados para a aclimatização de plântulas de batata. Miniestacas apicais podem ser utilizadas para a produção de mudas de batata, no sistema de enraizamento com substrato (areia), irrigadas com água de torneira ou solução nutritiva. O potencial de enraizamento das miniestacas é negativamente afetado pelo avanço da idade fisiológica da planta matriz.

A maioria das fruteiras nativas pertencem a família Myrtaceae, que apresentam como principal potencialidade a comercialização seus frutos. Apesar da grande aceitação dos frutos destas fruteiras, ainda é necessário a criação de pomares comerciais, pois prevalece-se o extrativismo. Para iniciar qualquer pomar, o primeiro ponto está na escolha da planta matriz, devendo esta apresentar características de superioridade quando comparadas aos outros genótipos. A partir disso, deve-se proceder na escolha do método que possibilite produzir quantidade de mudas satisfatória e de preferência sem perder as características da planta mãe. Para isso adota-se técnicas assexuadas, com opção para enxertia, estaquia e alporquia. Estas técnicas quando testadas com fruteiras nativas, mostraram-se limitantes em diversos aspectos, devendo-se testar outra que poderá ser recomendada para uso, principalmente naquelas de maior potencialidade como jabuticabeiras, pitangueiras, sete capoteiro e araçazeiro amarelo. O objetivo deste trabalho foi testar o uso da propagação assexuada por mini-estaquia nestas fruteiras, de acordo com época de coleta, comprimento da mini-estaca e concentração de AIB, bem como, relacionar tais resultados de rizogênese com triptofano extraído em determinados períodos. O trabalho foi realizado na Universidade Tecnológica Federal do Paraná - Câmpus Dois Vizinhos. As coletas foram realizadas bimestralmente. Foram preparadas mini-estacas com 6 ou 8 cm, com par de folhas reduzido a 25% do tamanho original. As mini-estacas tiveram sua base imersa em solução líquida de ácido indol-butírico (AIB), nas concentrações de 0, 3000 e 6000 mg L-1 e em seguida foram colocadas em tubetes contendo substrato comercial. O delineamento experimental foi inteiramente casualizado com fatorial 2 x 3 x 6 (comprimento de estaca x concentração de AIB x época do ano), com quatro repetições, sendo a unidade experimental variou de acordo com a quantidade de brotações obtidas em tais períodos. Aos 120 dias da implantação de cada coleta foram avaliados o enraizamento e calogênese (%), número médio de raízes por mini-estaca e comprimento médio das raízes. Após 60 dias destas análises avaliou-se a sobrevivência das mini-estacas enraizadas pós-plantio. Também avaliou-se a produção de mini-estacas de cada tamanho em cada época do ano. Ao final do experimento avaliou-se o percentual de sobrevivência das mudas matrizes. Para análise de triptofano utilizou-se materiais de ramos, folhas e ramos com folhas, retirados dos materiais utilizados para produção de mini-estacas. Recomenda-se para jabuticabeira híbrida o uso de mini-estacas com oito centímetros, tratadas com 6000 mg L-1 de AIB e coletadas em junho. Para jabuticabeira de cabinho e araçazeiro o período de coleta para propagação por mini-estaquia destas deve ser em agosto, independente da concentração de AIB e comprimento da mini-estaca. Com a jabuticabeira sabará e sete capoteiro é importante para obtenção de resultados mais satisfatórios a coleta sendo realizada tanto em outubro quanto em dezembro, com mesma independência dos outros níveis testados nos outros fatores. Contudo, para sete capoteiro deve-se testar outras técnicas para aumentar a eficiência da propagação. E com pitangueira recomenda-se coletar em junho, porém nas de 6 cm aplicação de 3000 mg L-1 de AIB e de 8 cm com 6000 mg L-1 de AIB.
The most native fruit trees are belonging to Myrtaceae family, which it have as main marketing potential their fruit. Despite the wide acceptance of the fruits of these native fruit cultura, the establishment of commercial orchards is still necessary, because if it prevails extraction in the forest. To start the cultivo in the orchard, the first point is on the mother plant choice, which should provide superior characteristics when compared to other genotypes. Then, it is necessary to choose the method to can produce satisfactory amount of seedlings and preferably without it to lose the mother plant characteristics. For this, it adopts the asexual thechniques, with option for grafting, cuttings and air layering. These techniques when tested with native fruits tree, it proved limiting in theses results, with this, it should to test other it to recommend its use, especially, those fruit native of higher potential as jabuticaba tree, pitanga tree, sete capote tree and araça amarelo tree. The aim of this study was to test the use of asexual propagation through mini-cuttings in these native fruit trees, according to the time of collection, the mini-cutting length and concentration of IBA, as well as, it to relate the results of rooting with tryptophan extracted at certain times. The work was carried out at Universidade Tecnológica Federal do Paraná – Câmpus Dois Vizinhos, Brazil. The samples were collected each two months. The mini-cutting were prepared with 6 or 8 cm, with a pair of leaves reduced to 25% of the original size. The mini-cuttings had their base immersed in liquid solution of indole-butyric acid (IBA) in the concentrations of 0, 3000 and 6000 mg L-1 and then were placed in tubes containing commercial substrate. The experimental design was completely randomized with factorial 2 x 3 x 6 (mini-cutting length x IBA concentration x time of collection), with four replications, it being each plot varied according to the amount of shoots obtained by period time. After 120 days, the rooting and callus formation (%), average number of roots per mini-cutting and the average length of the roots were evaluated. After 60 days of these evaluations, the survival of mini-cuttings rooted after transplant was evaluated. It was evaluated also the production of mini-cuttings of each size in each period time. At the end of the experiment it was evaluated the percentage of survival of mother plantlets. For analysis of tryptophan was used materials branches, leaves and twigs with leaves, taken from the materials used for the production of mini-cutting. It was recommended for hybrid jabuticaba tree the use mini-cutting with eight cm, treated with 6000 mg L-1 of IBA and collected in June. For jabuticaba tree of cabinho and araça amarelo tree the period for propagation by mini-cuttings should be in August, regardless of IBA concentration and length of the mini-cutting. In the jabuticaba tree sabara and sete capote tree is important to obtain more satisfactory results realized the collect in October or December, with the same independence of other levels tested in other factors. However, for sete capote tree should test other techniques to increase the efficiency of propagation. And with pitanga tree recommended to the collection in June, but with 6cm the application of 3000 mg L-1 of IBA and 8 cm with 6000 mg L-1 of IBA.