Bibliografías temáticas / Molecular gender determination

Índice

Artículos de revistas
Tesis
Libros
Capítulos de libros
Actas de conferencias
Informes

Literatura académica sobre el tema "Molecular gender determination"

Autor: Grafiati

Publicado: 4 de junio de 2021

Última modificación: 10 de febrero de 2022

Crea una cita precisa en los estilos APA, MLA, Chicago, Harvard y otros

Elija tipo de fuente:

Consulte las listas temáticas de artículos, libros, tesis, actas de conferencias y otras fuentes académicas sobre el tema "Molecular gender determination".

Junto a cada fuente en la lista de referencias hay un botón "Agregar a la bibliografía". Pulsa este botón, y generaremos automáticamente la referencia bibliográfica para la obra elegida en el estilo de cita que necesites: APA, MLA, Harvard, Vancouver, Chicago, etc.

También puede descargar el texto completo de la publicación académica en formato pdf y leer en línea su resumen siempre que esté disponible en los metadatos.

Artículos de revistas sobre el tema "Molecular gender determination"

Shaw, Carla N., Paul J. Wilson y Bradley N. White. "A RELIABLE MOLECULAR METHOD OF GENDER DETERMINATION FOR MAMMALS". Journal of Mammalogy 84, n.º 1 (febrero de 2003): 123–28. http://dx.doi.org/10.1644/1545-1542(2003)084<0123:armmog>2.0.co;2.

Texto completo

Los estilos APA, Harvard, Vancouver, ISO, etc.

Shahrul Hi, Z. A., S. Sahidan, M. A. W. Rohaya, M. Y. Siti Afeefah, Z. A. Intan Zari, J. A. N. Nor Hidaya, R. M. A. Nadiah y Z. A. Zaidah. "Molecular Gender Determination of Ancient Human from Malay Peninsular". American Journal of Applied Sciences 6, n.º 10 (1 de octubre de 2009): 1770–75. http://dx.doi.org/10.3844/ajassp.2009.1770.1775.

Texto completo

Los estilos APA, Harvard, Vancouver, ISO, etc.

Horng, Yan-Ming, Chean-Ping Wu, Yng-Chyu Wang y Mu-Chiou Huang. "A novel molecular genetic marker for gender determination of pigeons". Theriogenology 65, n.º 9 (junio de 2006): 1759–68. http://dx.doi.org/10.1016/j.theriogenology.2005.10.011.

Texto completo

Los estilos APA, Harvard, Vancouver, ISO, etc.

Hedges, Dale J., Jerilyn A. Walker, Pauline A. Callinan, Jaiprakash G. Shewale, Sudhir K. Sinha y Mark A. Batzer. "Mobile element-based assay for human gender determination". Analytical Biochemistry 312, n.º 1 (enero de 2003): 77–79. http://dx.doi.org/10.1016/s0003-2697(02)00430-x.

Texto completo

Los estilos APA, Harvard, Vancouver, ISO, etc.

Hutchison, J. B., A. Wozniak, C. Beyer, M. Karolczak y R. E. Hutchison. "Steroid metabolising enzymes in the determination of brain gender". Journal of Steroid Biochemistry and Molecular Biology 69, n.º 1-6 (abril de 1999): 85–96. http://dx.doi.org/10.1016/s0960-0760(99)00057-6.

Texto completo

Los estilos APA, Harvard, Vancouver, ISO, etc.

Filipovic, Gordana, Julija Radojicic, Maja Stosic, P. Janosevic y Zorica Ajdukovic. "Odontometric analysis of permanent canines in gender determination". Archives of Biological Sciences 65, n.º 4 (2013): 1279–83. http://dx.doi.org/10.2298/abs1304279f.

Texto completo

Los estilos APA, Harvard, Vancouver, ISO, etc.

Vainer, Olga B., Aleksey V. Katokhin, Sergey M. Kustov, Valentin V. Vlassov y Pavel P. Laktionov. "A New Y Chromosome Marker for Noninvasive Fetal Gender Determination". Annals of the New York Academy of Sciences 1137, n.º 1 (agosto de 2008): 157–61. http://dx.doi.org/10.1196/annals.1448.041.

Texto completo

Los estilos APA, Harvard, Vancouver, ISO, etc.

Khandka, Deepak K., Ali Nejidat, Moshe Tal y Avi Golan-Goldhirsh. "RAPD Marker for Sex Determination of Dioecious Plants". HortScience 30, n.º 4 (julio de 1995): 878F—878. http://dx.doi.org/10.21273/hortsci.30.4.878f.

Texto completo

Resumen

Several horticulturally important species are dioecious (e.g., pistachio, date palm, poplar, and others). It would be advantageous if the gender of a seedling could be determined at the vegetative stage. In this report, we present results of our search for molecular markers for sex differentiation in dioecious species. The method used was bulked segregant analysis of random amplified polymorphic DNA (RAPD) for sex. A male-specific marker fragment OPB01-1470 was obtained in Mercurialis annua. The sex linkage and characterization of this marker will be discussed.

Los estilos APA, Harvard, Vancouver, ISO, etc.

Bleher, R., W. Erwin, A. M. Paprocki, C. M. Syverson, R. Koppang y B. A. Didion. "259 A RAPID, NON-PCR-BASED BOVINE EMBRYO BIOPSY SEXING ASSAY". Reproduction, Fertility and Development 21, n.º 1 (2009): 227. http://dx.doi.org/10.1071/rdv21n1ab259.

Texto completo

Resumen

Sex determination of bovine embryos in conjunction with embryo transfer is an established method to maximize efficiency by producing offspring with the preferred gender. Present methodology for sexing embryos is based on PCR, which requires molecular laboratory equipment, trained personnel, and several hours before a result becomes known. We developed a simple, rapid, non-PCR procedure for identification of Y-chromosomes in bovine blastomeres recovered via biopsy. A biopsy (n = 5 to 8 blastomeres) was taken from a blastocyst-stage embryo and transferred onto a plastic slide. After drying and fixation, the cells were denatured and incubated with a peptide nucleic acid probe designed to target unique Y-chromosome specific sequence. The probe was conjugated to a fluorescent dye (CY-3) which enables Y-chromosome detection as a bright spot within blastomere nuclei when using a fluorescent microscope. The absence of signal indicates female embryonic DNA. From placement of the biopsy onto a plastic slide, the methodology required approximately 75 minutes to determine embryo gender. The accuracy of the biopsy sexing procedure was demonstrated by parallel gender determination of the same embryo using an established PCR method designed for the bovine amelogenin locus. Based on 18 in vitro-produced bovine embryos generating a result for both assays, there was a 94.4% match (17/18) of gender assignment. The present technology represents a simple alternative to PCR-based embryo sexing technology. Research is ongoing for future development of live embryo sexing determination.

Los estilos APA, Harvard, Vancouver, ISO, etc.

Allen, Caitilyn. "It's a Boy! Gender Expectations Intrude on the Study of Sex Determination". DNA and Cell Biology 26, n.º 10 (octubre de 2007): 699–705. http://dx.doi.org/10.1089/dna.2007.1501.

Texto completo

Los estilos APA, Harvard, Vancouver, ISO, etc.

Más fuentes

Tesis sobre el tema "Molecular gender determination"

Chapman, Alexandra. "Development of Novel High-Resolution Melting (HRM) Assays for Gender Identification of Caribbean Flamingo (Phoenicopterus ruber ruber) and other Birds". Thesis, 2012. http://hdl.handle.net/1969.1/148342.

Texto completo

Resumen

Unambiguous gender identification (ID) is needed to assess parameters in studies of population dynamics, behavior, and evolutionary biology of Caribbean Flamingo (Phoenicopterus ruber ruber) and other birds. Due to its importance for management and conservation, molecular (DNA-based) avian gender ID assays targeting intron-size differences of the Chromosome Helicase ATPase DNA Binding (CHD) gene of males (CHD-Z) and females (CHD-W) have been developed. Male (ZZ) and female (WZ) genotypes are usually scored as size polymorphisms through agarose or acrylamide gels. For certain species, W-specific restriction sites or multiplex polymerase chain-reaction (PCR) involving CHD-W specific primers are needed. These approaches involve a minimum of three steps following DNA isolation: PCR, gel electrophoresis, and photo-documentation, which limit high throughput scoring and automation potential. In here, a short amplicon (SA) High-resolution Melting Analysis (HRMA) assay for avian gender ID is developed. SA-HRMA of an 81-Base Pair (bp) segment differentiates heteroduplex female (WZ) from homoduplex male (ZZ) genotypes by targeting Single-nucleotide Polymorphisms (SNPs) instead of intron-size differences between CHD-Z and CHD-W genes. To demonstrate the utility of the approach, the gender of Caribbean Flamingo (P. ruber ruber) (17 captive from the Dallas Zoo and 359 wild from Ria Lagartos, Yucatan, Mexico) was determined. The assay was also tested on specimens of Lesser Flamingo (P. minor), Chilean Flamingo (P. chilensis), Saddle-billed Stork (Ephippiorhynchus senegalensis), Scarlet Ibis (Eudocimus ruber), White-bellied Stork (Ciconia abdimii), Roseate Spoonbill (Platalea ajaja), Marabou Stork (Leptoptilos crumeniferus), Greater Roadrunner (Geococcyx californianus), and Attwater's Prairie Chicken (Tympanuchus cupido attwateri). Although the orthologous 81 bp segments of Z and W are highly conserved, sequence alignments with 50 avian species across 15 families revealed mismatches affecting one or more nucleotides within the SA-HRMA forward or reverse primers. Most mismatches were located along the CHD-Z gene that may generate heteroduplex curves and thus gender ID errors. For such cases, taxon and species-specific primer sets were designed. The SA-HRMA gender ID assay can be used in studies of avian ecology and behavior, to assess sex-associated demographics and migratory patterns, and as a proxy to determine the health of the flock and the degree by which conservation and captive breeding programs are functioning.

Los estilos APA, Harvard, Vancouver, ISO, etc.

Libros sobre el tema "Molecular gender determination"

Browder, Leon W. The molecular biology of cell determination and cell differentiation. New York: Plenum Press, 1988.

Buscar texto completo

Los estilos APA, Harvard, Vancouver, ISO, etc.

Sex in fungi: Molecular determination and evolutionary implications. Washington, DC: ASM Press, 2007.

Buscar texto completo

Los estilos APA, Harvard, Vancouver, ISO, etc.

(Editor), Joseph Heitman, James Warren Kronstad (Editor), John W. Taylor (Editor) y Lorna A. Casselton (Editor), eds. Sex in Fungi: Molecular Determination and Evolutionary Implications. ASM Press, 2007.

Buscar texto completo

Los estilos APA, Harvard, Vancouver, ISO, etc.

Capítulos de libros sobre el tema "Molecular gender determination"

Hu, Dong Gui, Xin Yuan Guan y Nicole Hussey. "Gender Determination and Detection of Aneuploidy in Single Cells Using DNA Array-Based Comparative Genomic Hybridization". En Methods in Molecular Medicine™, 135–51. Totowa, NJ: Humana Press, 2007. http://dx.doi.org/10.1007/978-1-59745-298-4_12.

Texto completo

Los estilos APA, Harvard, Vancouver, ISO, etc.

Paro, Renato, Ueli Grossniklaus, Raffaella Santoro y Anton Wutz. "Dosage Compensation Systems". En Introduction to Epigenetics, 67–89. Cham: Springer International Publishing, 2021. http://dx.doi.org/10.1007/978-3-030-68670-3_4.

Texto completo

Resumen

AbstractThis chapter provides an introduction to chromosome-wide dosage compensation systems. We will examine the evolution of dosage compensation, which is thought to be driven by the appearance of differentiated sex chromosomes. In a subset of species with X chromosomal sex determination or XY sex chromosome systems, expression of X-linked genes is regulated by chromosome-wide modifications that equalize gene expression differences between males and females. The molecular mechanisms of X chromosome-wide dosage compensation have been studied in flies, worms, and mammals. Each of these species uses a distinct dosage compensation strategy with a different molecular mechanism. In the wormCaenorhabditis elegans, gene expression on the two X chromosomes of hermaphrodites is reduced to a level that approximates a single X chromosome in males. The fruit flyDrosophila melanogasterachieves dosage compensation by increased transcription of the single X chromosome in males to a level that is similar to the two X chromosomes in females. Lastly, in mammals, one of the two X chromosomes in female cells is transcriptionally inactive and a single X chromosome is transcribed in both sexes. Studies of dosage compensation systems provide insights into how epigenetic regulation controls gene expression and chromatin organization differentially within a cell.

Los estilos APA, Harvard, Vancouver, ISO, etc.

Ross, John, Igor Schreiber y Marcel O. Vlad. "Mini-Introduction to Bioinformatics". En Determination of Complex Reaction Mechanisms. Oxford University Press, 2006. http://dx.doi.org/10.1093/oso/9780195178685.003.0015.

Texto completo

Resumen

There is enormous interest in the biology of complex reaction systems, be it in metabolism, signal transduction, gene regulatory networks, protein synthesis, and many others. The field of the interpretation of experiments on such systems by application of the methods of information science, computer science, and biostatistics is called bioinformatics (see for a presentation of this subject). Part of it is an extension of the chemical approaches that we have discussed for obtaining information on the reaction mechanisms of complex chemical systems to complex biological and genetic systems. We present here a very brief introduction to this field, which is exploding with scientific and technical activity. No review is intended, only an indication of several approaches on the subject of our book, with apologies for the omission of vast numbers of publications. A few reminders: The entire complement of DNA molecules constitute the genome, which consists of many genes. RNA is generated from DNA in a process called transcription; the RNA that codes for proteins is known as messenger RNA, abbreviated tomRNA. Other RNAs code for functional molecules such as transfer RNAs, ribosomal components, and regulatory molecules, or even have enzymatic function. Protein synthesis is regulated by many mechanisms, including that for transcription initiation, RNA splicing (in eukaryotes), mRNA transport, translation initiation, post-translational modifications, and degradation of mRNA. Proteins perform perhaps most cellular functions. Advances in microarray technology, with the use of cDNA or oligonucleotides immobilized in a predefined organization on a solid phase, have led to measurements of mRNA expression levels on a genome-wide scale (see chapter 3). The results of the measurements can be displayed on a plot on which a row represents one gene at various times, a column the whole set of genes, and the time of gene expression is plotted along the axis of rows. The changes in expression levels, as measured by fluorescence, are indicated by colors, for example green for decreased expression, black for no change in expression, and red for increased expression. Responses in expression levels have been measured for various biochemical and physiological conditions. We turn now to a few methods of obtaining information on genomic networks from microarray measurements.

Los estilos APA, Harvard, Vancouver, ISO, etc.

"Analyzing Gene Expression through Real Time PCR while Neo-tissue Regeneration using Developed Tissue Constructs". En Protocols used in Molecular Biology, editado por Divakar Singh, Tarun Minocha, Satyavrat Tripathi, Rupika Sinha, Shubhankar Anand, Hareram Birla, Vivek Kumar Pandey et al., 15–34. BENTHAM SCIENCE PUBLISHERS, 2020. http://dx.doi.org/10.2174/9789811439315120010006.

Texto completo

Resumen

Real-time PCR offers a wide area of application to analyze the role of gene activity in various biological aspects at the molecular level with higher specificity, sensitivity and the potential to troubleshoot with post-PCR processing and difficulties. With the recent advancement in the development of functional tissue graft for the regeneration of damaged/diseased tissue, it is effective to analyze the cell behaviour and differentiation over tissue construct toward specific lineage through analyzing the expression of an array of specific genes. With the ability to collect data in the exponential phase, the application of Real-Time PCR has been expanded into various fields such as tissue engineering ranging from absolute quantification of gene expression to determine neo-tissue regeneration and its maturation. In addition to its usage as a research tool, numerous advancements in molecular diagnostics have been achieved, including microbial quantification, determination of gene dose and cancer research. Also, in order to consistently quantify mRNA levels, Northern blotting and in situ hybridization (ISH) methods are less preferred due to low sensitivity, poor precision in detecting gene expression at a low level. An amplification step is thus frequently required to quantify mRNA amounts from engineered tissues of limited size. When analyzing tissue-engineered constructs or studying biomaterials–cells interactions, it is pertinent to quantify the performance of such constructs in terms of extracellular matrix formation while in vitro and in vivo examination, provide clues regarding the performance of various tissue constructs at the molecular level. In this chapter, our focus is on Basics of qPCR, an overview of technical aspects of Real-time PCR; recent Protocol used in the lab, primer designing, detection methods and troubleshooting of the experimental problems.

Los estilos APA, Harvard, Vancouver, ISO, etc.

Michael Egbuche, Chukwudi. "Prompt and Accurate Diagnosis, A Veritable Tool in Malaria Elimination Efforts". En Current Topics and Emerging Issues in Malaria Elimination. IntechOpen, 2021. http://dx.doi.org/10.5772/intechopen.96582.

Texto completo

Resumen

The concept of malaria elimination is to get rid of local transmission of malaria parasites in a defined geographical area. Among the measures required for malaria elimination is prompt and accurate diagnosis. Malaria diagnostic tools currently in use: clinical diagnosis, Malaria Rapid Diagnostic Tests (mRDT) and molecular diagnosis, have limitations. Clinical diagnosis can be used as first step in making prompt malaria diagnosis, but cannot confirm cases. Malaria RDTs satisfies the need for prompt diagnosis but has low accuracy in confirming cases. Accuracy of microscopy depends on making good blood films, and accurate film interpretation. Molecular diagnosis required for species-specific diagnosis of malaria parasites, and determination of genes that confers drug resistance to Plasmodium species is not available for routine use. As part of elimination efforts, there is development of mRDT kits that utilize urine or saliva instead of blood specimen, microscopy digital image recognition and different technologies for molecular diagnosis. So far, none of these diagnostic tools has satisfied the need for prompt and accurate diagnosis. It is therefore recommended that more than one diagnostic tool is needed for malaria elimination to be achieved in a given area. This will ensure early detection and treatment of cases, as well as prevent the re-establishment of transmission.

Los estilos APA, Harvard, Vancouver, ISO, etc.

Rebić, Velma, Mufida Aljičević, Sajra Vinčević-Smajlović y Damir Rebić. "Distribution and Molecular Detection of Methicilin-Resistant Staphylococcus aureus". En Infectious Diseases and Sepsis [Working Title]. IntechOpen, 2021. http://dx.doi.org/10.5772/intechopen.98655.

Texto completo

Resumen

Isolation of Staphylococcus aureus is quite common in both the general population and hospital environment. The heterogeneity of the disease and the unique ability of S. aureus to develop resistance to the most recently discovered antibacterial drugs points to its ability to adapt and survive in different conditions. CA-MRSA is different from hospital strains of MRSA by its epidemiological, phenotypic and genotypic characteristics. The emergence of MRSA in the community suggests the need for a new approach to managing the indications and the certification of staphylococcal infections, with special emphasis on the selection of empiric antibiotic therapy. In the study, we analised of MRSA from 4341 samples taken from patients from the general population of Sarajevo Canton in the six-month period of follow-up processed at the Public Health Institute of Sarajevo Canton. We determined the epidemiological characteristics of the isolated strains. Methicillin resistance was determined by phenotypic methods. The following molecular methods were used for the confirmation of methicillin resistance: determination of the mecA gene, PFGE profile, genetic type of MRSA being determined by spa typing, the distribution of SCCmec types being examined, and the detected gene for PVL. The study stresses the need for national monitoring of spreading of the existing epidemic strains, as well as the monitoring of emergence of new strains which would enable the inclusion of our country in the international network of monitoring bacterial resistance.

Los estilos APA, Harvard, Vancouver, ISO, etc.

Masood, Shahla, Roberto Salgado, Peter Regitnig y Rudi Pauwels. "The Status of Breast Pathology around the Globe". En Breast cancer: Global quality care, editado por Hans Junkermann, Wolfgang Buchberger, Sylvia Heywang-Köbrunner, Michael Michell, Alexander Mundinger, Carol Benn y Sophia Zackrisson, 126–43. Oxford University Press, 2019. http://dx.doi.org/10.1093/med/9780198839248.003.0012.

Texto completo

Resumen

Abstract: Breast cancer has received remarkable attention at the global level, and as a result, substantial progress has been made over the past several years in the diagnosis and management of this disease. Emphasis on breast cancer screening and advances in breast imaging have led to increased early breast cancer detection. Minimally invasive sampling procedures such as fine needle aspiration biopsy and core needle biopsy have almost replaced open surgical biopsy. Breast conserving therapy and sentinel lymph node biopsy have become attractive alternatives to mastectomy and total axillary dissection. Advances in molecular diagnostics and targeted therapies have opened more effective options for individualised breast cancer therapy. In addition, discovery of breast cancer genes and recognition of breast cancer risk factors have provided opportunities for introducing various risk reduction modalities. Similarly, enhanced public awareness and the efforts of patient advocates have resulted in increased funding for breast cancer research. More importantly, integrated breast cancer care via a multidisciplinary approach has provided the foundation for the establishment of breast centres around the globe. The above-mentioned efforts have been complemented by the role that pathologists have played in the realization of these advancements. Pathologists have been central in the development, validation, implementation, and appropriateness of providing diagnostic and predictive/prognostic information. Throughout the years, pathologists have evolved from being a morphologist into becoming clinicians/scientists with in-depth understanding of integrated breast cancer care, research, and education. Currently, pathologists make the ultimate determination about the nature of a disease and help design the course of therapy for individualized patients.

Los estilos APA, Harvard, Vancouver, ISO, etc.

Aycan, Murat, Muhammet Cagri Oguz, Yasin Ozgen, Burak Onol y Mustafa Yildiz. "Gamma Radiation Effect on Agrobacterium tumefaciens-Mediated Gene Transfer in Potato (Solanum tuberosum L.)". En Solanum tuberosum - a Promising Crop for Starvation Problem [Working Title]. IntechOpen, 2021. http://dx.doi.org/10.5772/intechopen.99878.

Texto completo

Resumen

Potato (Solanum tuberosum L.) is one of the major crops of the world. Significant improvements can be achieved in terms of yield and quality by the determination of efficient transformation methods. On the other hand, low transformation frequency seriously limits the application of molecular techniques in obtaining transgenic crops. In the present study, the effect of gamma radiation on Agrobacterium tumefaciens-mediated transformation to the potato was firstly investigated. Sterile seedlings of potato cv. ‘Marabel’, which was grown on Gamborg’s B5 medium in Magenta vessels, were irradiated with different gamma radiation doses (0-control, 40, 80, 120 Gy 60Co). Stem parts having axillary meristems were excised from irradiated seedlings and inoculated by A. tumefaciens (GV2260), which harbors the binary plasmid p35S GUS-INT contains and GUS (β-glucuronidase) gene controlled by 35S promoter (CaMV) and nptII (neomycin phosphotransferase II) gene driven by NOS (nopaline synthase) promoter). Inoculated stem parts having axillary meristems explants were then directly transported to a selection medium containing duocid (500 mg l−1), and kanamycin (100 mg l−1), 4 mg l−1 gibberellic acid, 1 mg l−1 BAP and 0.1 mg l−1 NAA. The adult transgenic plants were detected by polymerase chain reaction (PCR) analysis. According to the number of transgenic plants determined by PCR analysis, results obtained from explants treated with 40 Gy gamma gave the best results compared to the control (0 Gy) application. The doses over 40 Gy were also found statistically significant compared to the control (0 Gy). It is expected that the protocol described in this study make the transformation studies easier by skipping the stages of ‘co-cultivation’, ‘culturing explants on selection medium’ and ‘recovery of transgenic shoots on selection medium’ not only for potato but also for other crop plants. This study was supported by a grant from the Scientific and Technological Research Council of Turkey (TUBİTAK) (Grant number 113O280 to Prof. Dr. Mustafa YILDIZ).

Los estilos APA, Harvard, Vancouver, ISO, etc.

Actas de conferencias sobre el tema "Molecular gender determination"

Cardenas-Manriquez, G., D. A. Robles-Bustos, I. Vega-Munoz, A. L. Villagomez-Aranda, I. Torres-Pacheco, R. G. Guevara-Olvera, A. Hernandez-Cruz y M. M. Gonzalez-Chavira. "Determination of molecular comunication network in transgenic tobbaco expressing CchGLP gene". En 2017 XIII International Engineering Congress (CONIIN). IEEE, 2017. http://dx.doi.org/10.1109/coniin.2017.7968197.

Texto completo

Los estilos APA, Harvard, Vancouver, ISO, etc.

Pannekoek, H., M. Linders, J. Keijer, H. Veerman, H. Van Heerikhuizen y D. J. Loskutoff. "THE STRUCTURE OF THE HUMAN ENDOTHELIAL PLASMINOGEN ACTIVATOR INHIBITOR (PAI-1) GENE: NON-RANDOM POSITIONING OF INTRONS". En XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1644767.

Texto completo

Resumen

The endothelium plays a crucial role in the regulation of the fibrinolytic process, since it synthesizes and secretes tissue-type plasminogen activator (t-PA) as well as the fast-acting plasminogen activator inhibitor (PAI-1). Molecular cloning of full-length PAI-1 cDNA, employing a human endothelial cDNA expression library, and a subsequent determination of the complete nucleotide sequence, allowed a prediction of the amino-acid sequence of the PAI-1 glycoprotein. It was observed that the amino-acid sequence is significantly homologous to those of members of the serine protease inhibitor ("Serpin") family, e.g. αl-antitrypsin and antithrombin III. Serpins are regulators of various processes, such as coagulation, inflammatory reactions, complement activation and share a common functional principle and a similar structure, indicative for a common primordial gene. The intron-exon arrangement of Serpin genes may provide a record for the structure of a primordial gene. A comparison of the location of introns among members of the Serpin family reveals that some introns are indeed present at identical or almost identical positions, however in many other cases there is no correspondence between the intron positions among different Serpin genes.Obviously, more data on the chromosomal gene structure of members of this family are required to formulate a scheme for the evolutionary creation of the Serpins. To that end, we have established the number and the precise location of the introns in the PAI-1 gene and have compared these data with those reported on other Serpin genes. For that purpose a human genomic cosmid DNA library of about 340.000 independent colonies was screened with radiolabelled full-length PAI-1 cDNA as probe. Two clones were found which contain the entire PAI-1 gene. Restriction site mapping, electron microscopic inspection of heteroduplexes and nucleotide sequence analysis demonstrate that the PAI-1 gene comprises about 12.2kilo basepairs and consists of nine exons and eight introns. Intron-exon boundaries are all in accord with the "GT-AG" rule, including a cryptic acceptor splice site found in intron 7. Furthermore, it is observed that intron 3 of the PAI-1 gene occupies an identical position as intron E of chicken ovalbumin and intron E of the ovalbumin-related gene Y. The location of the other seven introns is unrelated to the known location of introns in the genes encoding the Serpins, rat angiotensin, chicken ovalbumin (and gene Y), human antithrombin III and human al-antitrypsin. The 3' untranslated region of the PAI-1 gene is devoid of introns, indicating that the two mRNA species detected in cultured endothelial cells which share an identical 5' untranslated segment and codogenic region, but differ in the length of the 3' untranslated region, arise by alternative polyadenylation. An extrapolation of the position of the introns to the amino-acid sequence of PAI-1, and adaption of the view that the subdomain structure of the Serpins is analogous, shows that the introns of PAI-1 are non-randomly distributed. Except for intron 7, the position of the other seven introns corresponds with randon-coil regions of the protein or with the borders of β-sheets and a-helices. Extrapolation of the position of introns in the genes of other Serpins to their respective amino-acid sequences and subdomain structures also reveals a preference for random-coil regions and borders of subdomains. These observations are reminiscent of an evolutionary model, called "intron sliding", that accounts for variations in surface loops of the same protein in different species by aberrant splicing (Craik et al., Science 220 (1983) 1125). The preferential presence of introns in gene segments, encoding these variable regions, and absence in regions determining the general folding of these proteins would explain conservation of the structure during the evolution of those genes.

Los estilos APA, Harvard, Vancouver, ISO, etc.

"Molecular markers based on SNPs in FAD3 genes for determination of linolenic acid content in flax seed". En Bioinformatics of Genome Regulation and Structure/ Systems Biology. institute of cytology and genetics siberian branch of the russian academy of science, Novosibirsk State University, 2020. http://dx.doi.org/10.18699/bgrs/sb-2020-211.

Texto completo

Los estilos APA, Harvard, Vancouver, ISO, etc.

KAYA, Yalcin, Caglar COLAK, Veli PEKCAN, Mehmet Ibrahim YILMAZ y Goksel EVCI. "THE DETERMINATION OF OLEIC ACID CONTENTS IN SUNFLOWER GENOTYPES". En RURAL DEVELOPMENT. Aleksandras Stulginskis University, 2018. http://dx.doi.org/10.15544/rd.2017.060.

Texto completo

Resumen

High oleic sunflower is new trend both in Turkey and also in the world due to that it present healthy vegetable oil and also higher standing ability for frying. Higher oleic acid also affects from environment especially nigh temperatures during the grain filling period but genetic contribution is also important. High oleic acid content comes from Pervenent mutation in sunflower and it controlling Ol genes. However, because of being a seed trait which is determining after harvest, it is so difficult and unnecessary works (waiting even low oleic ones until seed treshing, etc) to select high oleic sunflower genetic materials. Therefore, selection utilization of molecular markers for determining of higher oleic types help breeders a lot to select accurately high oleic ones and also reduce costs both workers, isolation material, etc… The study covers determining of higher oleic type sunflower genetic materials developed in National Sunflower Hybrid Breeding Project conducted by Trakya Agricultural Research Institute. To screen of high oleic acid genotypes, around 400 sunflower F2 and F3 individuals obtained from crosses between high oleic acid and low oleic acid lines were used in TUBITAK (The Scientific and Technological Research Council of Turkey) Project 1003-114O971. Fatty acids of sunflower genotypes were determined by Agilent 6850 Gas Chromatography in Trakya University Lab. Based on the study results, oleic acid contents of sunflower genotypes were changed between 21.9-91.8 %, linoleic acid contents of them between 1.1-66.5 %, palmitic acid contents of them were between 3.4-8.0 % and stearic acid contents of genotypes were changed between 1.1-9.7 %. The higher oleic types were selected based on the study results for further generations.

Los estilos APA, Harvard, Vancouver, ISO, etc.

Chen, Hsiu-hung Simon, Zhiquan Shu, Lei Cheng y Dayong Gao. "Development of a Microfluidic Injection and Perfusion Device for Single Cell Study". En ASME 2010 First Global Congress on NanoEngineering for Medicine and Biology. ASMEDC, 2010. http://dx.doi.org/10.1115/nemb2010-13317.

Texto completo

Resumen

The cell membrane, composed primarily of proteins and lipids, is a selectively permeable lipid bilayer in the scale of 10 nm or so. Molecules permeating through cell membranes play critical roles in the applications of drug delivery, cell therapy, and cryopreservation. Cryopreservation and banking of cells, such as umbilical cord bloods, female eggs, etc., are critical to facilitate practical and effective in vitro fertilization (IVF). The determination of molecule transport properties of cells, such as water and cryoprotectants (CPAs), is indispensable for developing optimal conditions for cryopreserving them. On the other hand, injection of material of interests, such as sperms and DNA segments, to female eggs or blastocysts, so-called intracytoplasmic sperm injection (ICSI) technique, are playing important roles on IVF and advanced gene knock-out. In this study, a novel micro-nano-fluidic system that allows perfusion and injection in nano-liter scale has been developed and fabricated by soft lithographic methods. A single cell in the microfluidic system is able to be trapped on site and then either be perfused by various solutions or injected with plain solutions or solutions with genetic materials. Our ongoing study will demonstrate that the micro-nano-fluidic system allows us to: 1) confine cells in a channel; 2) deliver drugs by perfusing the cell; 3) monitor osmotic behaviors of the cell by replacing its extracellular environment; and 4) perform ICSI with sperms or genetic materials.

Los estilos APA, Harvard, Vancouver, ISO, etc.

Segura Moreno, Yenifer Yamile, María Carolina SANABRIA SALAS, Jorge Andrés MESA LÓPEZ DE MESA, Rodolfo VARELA RAMIREZ, Natalia Lizeth ACOSTA VEGA y Martha Lucia SERRANO. "Abstract 3142: Determination of molecular patterns associated with the expression of the TMPRSS2-ERG fusion and ERG, EZH2, NKX3.1 and SPINK-1 genes to evaluate the clonal origin of multifocal prostate cancer and its association with disease progression". En Proceedings: AACR Annual Meeting 2021; April 10-15, 2021 and May 17-21, 2021; Philadelphia, PA. American Association for Cancer Research, 2021. http://dx.doi.org/10.1158/1538-7445.am2021-3142.

Texto completo

Los estilos APA, Harvard, Vancouver, ISO, etc.

Handa, M., K. Titani, K. Takio y Z. M. Ruggeri. "CHARACTERIZATION OF THE VON WILLEBRAND FACTOR-BINDING DOMAIN OF PLATELET MEMBRANE GLYCOPROTEIN Ib". En XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1642925.

Texto completo

Resumen

We have previously obtained immunochemical evidence that the von Willebrand factor (vWF)-binding domain of the platelet membrane glycoprotein (GP) Ib is located near the amino terminus of the a subunit (Journal of Biological Chemistry 261: 12579-12585, 1986). We have now determined the complete amino acid sequence of the 45 kDa tryptic fragment of glycocalicin that contains this domain. Purified glycocalicin was subjected to limited digestion with trypsin and the proteolytic fragments were separated by size-exclusion high-pressure liquid chromatography. Two fragments of 45 kDa and 84 kDa, respectively, were obtained under nonreducing conditions. After reduction and S-carboxymethylation, the 84 kDa fragment was unchanged, while the 45 kDa fragment yielded two new fragments, one of 35 kDa and the other of 7 kDa. This finding proves the existence of a trypsin cleavage site within a disulfide loop. Two primary sets of overlapping fragments were obtained by cleavage of the carboxymethylated protein at methionyl and lysyl bonds following treatment with cyanogen bromide and Achromobacter protease I, respectively. Additional fragments were obtained by treatment of glycocalicin with Staphylococcus aureus V8 protease and Serratia marcescens protease. Analysis of all these fragments provided data that allowed determination of the sequence of the amino terminal 299 residues of the GP Ib a-chain. This includes the 45 kDa tryptic fragment containing the vWF-binding domain. This 299-residue sequence, corresponding approximately to two thirds of the α-chain polypeptide, is largely hydrophobic and contains only two N-linked and one O-linked carbohydrate chains. A hydrophilic region exists between residues 215-299, with a cluster of ten negatively charged residues at 269-287. This area is likely to attract positively charged molecules. The hydrophilic, highly glycosylated (at Ser/Thr residues) region corresponding to the previously described "macroglycopeptide" begins at residue 292. The determined sequence of glycocalicin contains a region with seven repeats, indicative of gene duplication, and is highly homologous to human leucine-rich α2-glycoprotein.

Los estilos APA, Harvard, Vancouver, ISO, etc.

Informes sobre el tema "Molecular gender determination"

Gaul, Stephen B., Isabel T. Harris y D. L. Hank Harris. Molecular Characterization of Multidrug Resistant Salmonella Isolates From a Single Finisher Building for Determination of Horizontal Transmission of Resistance Genes. Ames (Iowa): Iowa State University, enero de 2005. http://dx.doi.org/10.31274/ans_air-180814-1094.

Texto completo

Los estilos APA, Harvard, Vancouver, ISO, etc.

Ofrecemos descuentos en todos los planes premium para autores cuyas obras están incluidas en selecciones literarias temáticas. ¡Contáctenos para obtener un código promocional único!