Bibliografías temáticas / Motilität, Adsorption

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Literatura académica sobre el tema "Motilität, Adsorption"

Autor: Grafiati

Publicado: 4 de junio de 2021

Última modificación: 18 de febrero de 2022

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Artículos de revistas sobre el tema "Motilität, Adsorption"

Röttgermann, Peter J. F., Samira Hertrich, Ida Berts, Max Albert, Felix J. Segerer, Jean-François Moulin, Bert Nickel y Joachim O. Rädler. "Cell Motility on Polyethylene Glycol Block Copolymers Correlates to Fibronectin Surface Adsorption". Macromolecular Bioscience 14, n.º 12 (10 de septiembre de 2014): 1755–63. http://dx.doi.org/10.1002/mabi.201400246.

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Liu, Wei, Yu Sun, Rimin Shen, Xiaoxiao Dang, Xiaolin Liu, Fu Sui, Yan Li et al. "A Chemotaxis-Like Pathway of Azorhizobium caulinodans Controls Flagella-Driven Motility, Which Regulates Biofilm Formation, Exopolysaccharide Biosynthesis, and Competitive Nodulation". Molecular Plant-Microbe Interactions® 31, n.º 7 (julio de 2018): 737–49. http://dx.doi.org/10.1094/mpmi-12-17-0290-r.

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The genome of the Azorhizobium caulinodans ORS571 contains a unique chemotaxis gene cluster (che) including five chemotaxis genes: cheA, cheW, cheY1, cheB, and cheR. Analysis of the role of the chemotaxis cluster of A. caulinodans using deletion mutant strains revealed that CheA or the Che signaling pathway controls chemotaxis behavior and flagella-driven motility and plays important roles in formation of biofilms and production of extracellular polysaccharides (EPS). Furthermore, the deletion mutants (ΔcheA and ΔcheA-R) were defective in competitive adsorption and colonization on the root surface of host plants. In addition, a functional CheA or Che pathway promoted competitive nodulation on roots and stems. Interestingly, a nonflagellated mutant, ΔfliM, displayed a phenotype highly similar to that of the ΔcheA or ΔcheA-R mutant strains. These findings suggest that through controlling flagella-driven motility behavior, the chemotaxis signaling pathway in A. caulinodans coordinates biofilm formation, EPS, and competitive colonization and nodulation.

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Wolkin, R. H. y J. L. Pate. "Phage Adsorption and Cell Adherence Are Motility-dependent Characteristics of the Gliding Bacterium Cytophaga johnsonae". Microbiology 132, n.º 2 (1 de febrero de 1986): 355–67. http://dx.doi.org/10.1099/00221287-132-2-355.

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Camper, Anne K., Jason T. Hayes, Paul J. Sturman, Warren L. Jones y Alfred B. Cunningham. "Effects of Motility and Adsorption Rate Coefficient on Transport of Bacteria through Saturated Porous Media". Applied and Environmental Microbiology 59, n.º 10 (1993): 3455–62. http://dx.doi.org/10.1128/aem.59.10.3455-3462.1993.

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Baldvinsson, Signe Berg, Martine C. Holst Sørensen, Christina S. Vegge, Martha R. J. Clokie y Lone Brøndsted. "Campylobacter jejuni Motility Is Required for Infection of the Flagellotropic Bacteriophage F341". Applied and Environmental Microbiology 80, n.º 22 (26 de septiembre de 2014): 7096–106. http://dx.doi.org/10.1128/aem.02057-14.

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ABSTRACTPrevious studies have identified a specific modification of the capsular polysaccharide as receptor for phages that infectCampylobacter jejuni. Using acapsularkpsMmutants ofC. jejunistrains NCTC11168 and NCTC12658, we found that bacteriophage F341 infectsC. jejuniindependently of the capsule. In contrast, phage F341 does not infectC. jejuniNCTC11168 mutants that either lack the flagellar filaments (ΔflaAB) or that have paralyzed, i.e., nonrotating, flagella (ΔmotAand ΔflgP). ComplementingflgPconfirmed that phage F341 requires rotating flagella for successful infection. Furthermore, adsorption assays demonstrated that phage F341 does not adsorb to these nonmotileC. jejuniNCTC11168 mutants. Taken together, we propose that phage F341 uses the flagellum as a receptor. Phage-host interactions were investigated using fluorescence confocal and transmission electron microscopy. These data demonstrate that F341 binds to the flagellum by perpendicular attachment with visible phage tail fibers interacting directly with the flagellum. Our data are consistent with the movement of theC. jejuniflagellum being required for F341 to travel along the filament to reach the basal body of the bacterium. The initial binding to the flagellum may cause a conformational change of the phage tail that enables DNA injection after binding to a secondary receptor.

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Bosakova, Tereza, Antonin Tockstein, Natasa Sebkova, Ondrej Simonik, Hana Adamusova, Jana Albrechtova, Tomas Albrecht, Zuzana Bosakova y Katerina Dvorakova-Hortova. "New Insight into Sperm Capacitation: A Novel Mechanism of 17β-Estradiol Signalling". International Journal of Molecular Sciences 19, n.º 12 (12 de diciembre de 2018): 4011. http://dx.doi.org/10.3390/ijms19124011.

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17β-estradiol (estradiol) is a natural estrogen regulating reproduction including sperm and egg development, sperm maturation—called capacitation—and sperm–egg communication. High doses can increase germ cell apoptosis and decrease sperm count. Our aim was to answer the biological relevance of estradiol in sperm capacitation and its effect on motility and acrosome reaction to quantify its interaction with estrogen receptors and propose a model of estradiol action during capacitation using kinetic analysis. Estradiol increased protein tyrosine phosphorylation, elevated rate of spontaneous acrosome reaction, and altered motility parameters measured Hamilton-Thorne Computer Assisted Semen Analyzer (CASA) in capacitating sperm. To monitor time and concentration dependent binding dynamics of extracellular estradiol, high-performance liquid chromatography with tandem mass spectrometry was used to measure sperm response and data was subjected to kinetic analysis. The kinetic model of estradiol action during sperm maturation shows that estradiol adsorption onto a plasma membrane surface is controlled by Langmuir isotherm. After, when estradiol passes into the cytoplasm, it forms an unstable adduct with cytoplasmic receptors, which display a signalling autocatalytic pattern. This autocatalytic reaction suggests crosstalk between receptor and non-receptor pathways utilized by sperm prior to fertilization.

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Klos, Antoine, Xxx Sedao, Tatiana E. Itina, Clémentine Helfenstein-Didier, Christophe Donnet, Sylvie Peyroche, Laurence Vico, Alain Guignandon y Virginie Dumas. "Ultrafast Laser Processing of Nanostructured Patterns for the Control of Cell Adhesion and Migration on Titanium Alloy". Nanomaterials 10, n.º 5 (30 de abril de 2020): 864. http://dx.doi.org/10.3390/nano10050864.

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Femtosecond laser texturing is a promising surface functionalization technology to improve the integration and durability of dental and orthopedic implants. Four different surface topographies were obtained on titanium-6aluminum-4vanadium plates by varying laser processing parameters and strategies: surfaces presenting nanostructures such as laser-induced periodic surface structures (LIPSS) and ‘spikes’, associated or not with more complex multiscale geometries combining micro-pits, nanostructures and stretches of polished areas. After sterilization by heat treatment, LIPSS and spikes were characterized to be highly hydrophobic, whereas the original polished surfaces remained hydrophilic. Human mesenchymal stem cells (hMSCs) grown on simple nanostructured surfaces were found to spread less with an increased motility (velocity, acceleration, tortuosity), while on the complex surfaces, hMSCs decreased their migration when approaching the micro-pits and preferentially positioned their nucleus inside them. Moreover, focal adhesions of hMSCs were notably located on polished zones rather than on neighboring nanostructured areas where the protein adsorption was lower. All these observations indicated that hMSCs were spatially controlled and mechanically strained by the laser-induced topographies. The nanoscale structures influence surface wettability and protein adsorption and thus influence focal adhesions formation and finally induce shape-based mechanical constraints on cells, known to promote osteogenic differentiation.

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Ollero, M., N. García-López, R. Pérez-Pé, J. A. Cebrián-Pérez y T. Muiño-Blanco. "Surface changes of ram spermatozoa by adsorption of homologous and heterologous seminal plasma proteins revealed by partition in an aqueous two-phase system". Reproduction, Fertility and Development 9, n.º 4 (1997): 381. http://dx.doi.org/10.1071/r96037.

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Ram spermatozoa freed from seminal plasma by a dextran ‘swim-up’ procedure were incubated with Tween 20 and fractionated into motile (PEG-rich) and stationary (dextran-rich) fractions by centrifugal countercurrent distribution (CCCD) in an aqueous dextran–Ficoll– polyethylene glycol (PEG) two-phase system. Increasing concentrations of Tween 20 resulted in greater amounts of extracted protein and lower cell viability. Addition of bull seminal plasma increased the proportion of live cells, whereas ram seminal plasma increased the proportion of stationary cells. Proteins isolated from each type of seminal plasma restored the CCCD profile of treated spermatozoa to the right, this effect being reduced when proteins were thermally denatured. Bovine serum albumin induced a slight displacement to the left. No restoration of profile was achieved when ram spermatozoa were exposed to proteins from bull seminal plasma in the presence of protein-free ram seminal plasma. Adsorption of seminal plasma proteins by spermatozoa previously stripped of surface proteins by exposure to detergent reversed the detergent effect on motility. The findings are consistent with the concept that ram seminal plasma contains a factor that interferes with protein adsorption on the cell surface and prevents the protective effect of seminal plasma proteins on maintenance of cell viability

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Gu, Yian, Yugang Hou, Dapeng Huang, Zhexia Hao, Xiaofang Wang, Zhong Wei, Alexandre Jousset et al. "Application of biochar reduces Ralstonia solanacearum infection via effects on pathogen chemotaxis, swarming motility, and root exudate adsorption". Plant and Soil 415, n.º 1-2 (29 de diciembre de 2016): 269–81. http://dx.doi.org/10.1007/s11104-016-3159-8.

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Zhang, Yue, Tai Li Sun, Qi Dong Li y Xi Chuan Zhang. "A Capillary Model for Gasesas Coolant and Lubricant in Metal Cutting". Key Engineering Materials 458 (diciembre de 2010): 167–72. http://dx.doi.org/10.4028/www.scientific.net/kem.458.167.

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The gases of nitrogen, oxygen, argon and carbon dioxide were employed as coolant and lubricant in metal cutting. Therefore, the smaller kinetic diameter of gas, the lower cutting forces can be obtained. A conical capillary model is proposed, based on the experimental results and the theory analysis of stress distributions at the tool-chip interface. According to the boundary-layer theory, the kinetics equations of gas flow were solute. The velocity and flux of gas molecule are presented. In the capillary, the adsorption of tool-chip interface results in boundary lubricating film; the conical shape of capillary limits the depth of coolant and lubricant penetrating; and the negative press is the motility for coolant and lubricant penetrating. The study results show the molecule size of the coolant and lubricant could effect on its performances, and the coolant and lubricant with a relatively small molecule may have the particularly effective nature, in metal cutting.

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Más fuentes

Tesis sobre el tema "Motilität, Adsorption"

Kraikivski, Pavel. "Non-equilibrium dynamics of adsorbed polymers and filaments". Phd thesis, Universität Potsdam, 2005. http://opus.kobv.de/ubp/volltexte/2005/597/.

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In the present work, we discuss two subjects related to the nonequilibrium dynamics of polymers or biological filaments adsorbed to two-dimensional substrates.

The first part is dedicated to thermally activated dynamics of polymers on structured substrates in the presence or absence of a driving force. The structured substrate is represented by double-well or periodic potentials. We consider both homogeneous and point driving forces. Point-like driving forces can be realized in single molecule manipulation by atomic force microscopy tips. Uniform driving forces can be generated by hydrodynamic flow or by electric fields for charged polymers.

In the second part, we consider collective filament motion in motility assays for motor proteins, where filaments glide over a motor-coated substrate. The model for the simulation of the filament dynamics contains interactive deformable filaments that move under the influence of forces from molecular motors and thermal noise. Motor tails are attached to the substrate and modeled as flexible polymers (entropic springs), motor heads perform a directed walk with a given force-velocity relation. We study the collective filament dynamics and pattern formation as a function of the motor and filament density, the force-velocity characteristics, the detachment rate of motor proteins and the filament interaction. In particular, the formation and statistics of filament patterns such as nematic ordering due to motor activity or clusters due to blocking effects are investigated. Our results are experimentally accessible and possible experimental realizations are discussed.
In der vorliegenden Arbeit behandeln wir zwei Probleme aus dem Gebiet der Nichtgleichgewichtsdynamik von Polymeren oder biologischen Filamenten, die an zweidimensionale Substrate adsorbieren.

Der erste Teil befasst sich mit der thermisch aktivierten Dynamik von Polymeren auf strukturierten Substraten in An- oder Abwesenheit einer treibenden Kraft. Das strukturierte Substrat wird durch Doppelmulden- oder periodische Potentiale dargestellt. Wir betrachten sowohl homogene treibende Kräfte als auch Punktkräfte. Punktkräfte können bei der Manipulation einzelner Moleküle mit die Spitze eines Rasterkraftmikroskops realisiert werden. Homogene Kräfte können durch einen hydrodynamischen Fluss oder ein elektrisches Feld im Falle geladener Polymere erzeugt werden.

Im zweiten Teil betrachten wir die kollektive Bewegung von Filamenten in Motility-Assays, in denen Filamente über ein mit molekularen Motoren überzogenes Substrat gleiten. Das Modell zur Simulation der Filamentdynamik beinhaltet wechselwirkende, deformierbare Filamente, die sich unter dem Einfluss von Kräften, die durch molekulare Motoren erzeugt werden, sowie thermischem Rauschen bewegen. Die Schaftdomänen der Motoren sind am Substrat angeheftet und werden als flexible Polymere (entropische Federn) modelliert. Die Kopfregionen der Motoren vollführen eine gerichtete Schrittbewegung mit einer gegebenen Kraft-Geschwindigkeitsbeziehung. Wir untersuchen die kollektive Filamentdynamik und die Ausbildung von Mustern als Funktion der Motor- und der Filamentdichte, der Kraft-Geschwindigkeitscharakteristik, der Ablöserate der Motorproteine und der Filamentwechselwirkung. Insbesondere wird die Bildung und die Statistik der Filamentmuster, wie etwa die nematische Anordnung aufgrund der Motoraktivität oder die Clusterbildung aufgrund von Blockadeeffekten, untersucht. Unsere Ergebnisse sind experimentell zugänglich und mögliche experimentelle Realisierungen werden diskutiert.

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Persson, Malin. "Characterization and optimization of the in vitro motility assay for fundamental studies of myosin II". Doctoral thesis, Linnéuniversitetet, Institutionen för kemi och biomedicin (KOB), 2013. http://urn.kb.se/resolve?urn=urn:nbn:se:lnu:diva-25241.

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Myosin II is the molecular motor responsible for muscle contraction. It transforms the chemical energy in ATP into mechanical work while interacting with actin filaments in so called cross-bridge cycles. Myosin II or its proteolytic fragments e.g., heavy meromyosin (HMM) can be adsorbed to moderately hydrophobic surfaces in vitro, while maintaining their ability to translocate actin filaments. This enables observation of myosin-induced actin filament sliding in a microscope. This “in vitro motility assay” (IVMA) is readily used in fundamental studies of actomyosin, including studies of muscle contraction. The degree of correlation of the myosin II function in the IVMA with its function in muscle depends on how the myosin molecules are arranged on the surface. Therefore a multi-technique approach, including total internal reflection spectroscopy, fluorescence interference contrast microscopy and quartz crystal microbalance with dissipation, was applied to characterize the HMM surface configurations. Several configurations with varying distributions were identified depending on the surface property. The most favorable HMM configurations for actin binding were observed on moderately hydrophobic surfaces. The effects on actomyosin function of different cargo sizes and amount of cargo loaded on an actin filament were also investigated. No difference in sliding velocities could be observed, independent of cargo size indicating that diffusional processive runs of myosin II along an actin filament are not crucial for actomyosin function in muscle. Furthermore, a tool for accurate velocity measurements appropriate for IVMAs at low [MgATP] was developed by utilizing the actin filament capping protein CapZ. These improvements allowed an investigation of the [MgATP]-velocity relationship to study possible processivity in fast skeletal muscle myosin II. It is shown that the [MgATP]–velocity relationship is well described by a Michaelis-Menten hyperbola. In addition, statistical cross-bridge modeling showed that the experimental results are in good agreement with recent findings of actomyosin cross-bridge properties, e.g., non-linear cross-bridge elasticity. However, no effect of inter-head cooperativity could be observed. In conclusion, the described results have contributed to in-depth understanding of the actomyosin cross-bridge cycle in muscle contraction.

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Actas de conferencias sobre el tema "Motilität, Adsorption"

Regis, Shawn, Manisha Jassal, Sina Youssefian, Nima Rahbar y Sankha Bhowmick. "Quantitative Studies of Fibronectin Adsorption on Submicron Scaffolds". En ASME 2012 Summer Bioengineering Conference. American Society of Mechanical Engineers, 2012. http://dx.doi.org/10.1115/sbc2012-80633.

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Fibronectin plays a crucial role in adhesion of several cell types, mainly due to the fact that it is recognized by at least ten different integrin receptors. Since most cell types can bind to fibronectin, it becomes involved in many various biological processes. The interaction of cells with ECM proteins such as fibronectin provides the signals affecting morphology, motility, gene expression, and survival of cells [1]. Fibronectin exists in both soluble and insoluble forms; soluble fibronectin is secreted by cells and exits in cell media or body fluids, whereas insoluble fibronectin exists in tissues or the extracellular matrix of cultured cells [2]. The ability to control adsorption of fibronectin on tissue engineering scaffolds would therefore play a huge role in controlling cell attachment and survival in vivo. This can be achieved through surface functionalization of the scaffolds. The goal of these studies is to use molecular dynamics (MD) simulations to mechanistically understand how fibronectin adsorption is enhanced by surface functionalization of submicron scaffolds.

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