Literatura académica sobre el tema "Patch-Clamp Techniken"
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Artículos de revistas sobre el tema "Patch-Clamp Techniken":
Lübke, Joachim y Dirk Feldmeyer. "Morphologie und synaptische Interaktion von Neuronen einer kortikalen Kolumne". e-Neuroforum 10, n.º 3 (1 de enero de 2004). http://dx.doi.org/10.1515/nf-2004-0302.
Tesis sobre el tema "Patch-Clamp Techniken":
Tredt, Christian. "Die Kinetik des heteromeren 5-HT3-Rezeptors: Untersuchungen mit Patch-Clamp-Technik und Computersimulationen". Diss., lmu, 2004. http://nbn-resolving.de/urn:nbn:de:bvb:19-24269.
Röder, Alexander. "Blockadeeffekte von halogenierten Strukturanaloga des Anästhetikums Propofol an spannungskontrollierten Natriumkanälen erarbeitet mit der Hilfe der Patch-Clamp-Technik". Hannover Bibliothek der Medizinischen Hochschule Hannover, 2010. http://d-nb.info/1002368987/34.
Haseneder, Rainer. "Wie verstärken Lachgas, Xenon und Isofluran die GABAA-Rezeptor-vermittelte zentrale Hemmung? Untersuchungen mit der Patch-Clamp-Technik /". [S.l.] : [s.n.], 2003. http://deposit.ddb.de/cgi-bin/dokserv?idn=968919332.
Eder, Claudia. "Ionenkanäle in deaktivierter Mikroglia". Doctoral thesis, Humboldt-Universität zu Berlin, Medizinische Fakultät - Universitätsklinikum Charité, 2001. http://dx.doi.org/10.18452/13736.
The current work describes patch clamp recordings in cultured murine microglial cells, which had been deactivated following exposure to astrocyte-conditioned medium. Deactivated microglial cells expressed inwardly rectifying, outwardly rectifying and calcium-activated potassium channels. The outwardly rectifying potassium channels were upregulated following treatment of microglial cells with the astrocyte-conditioned medium. The anti-inflammatory cytokine transforming growth factor was released by astrocytes and appeared to be responsible for the observed upregulation of outwardly rectifying potassium channels in deactivated microglia. In addition, deactivated microglial cells expressed chloride channels, which were found to be involved in microglial ramification, i.e., in the transformation of microglial cells from ameboid into ramified morphology. Voltage-gated proton channels, which are involved in processes of oxygen radical generation, were downregulated in deactivated microglial cells.
Brünker, Sandra. "Differentielle pharmakologische Sensitivität von humanen 5-HT3-Rezeptor-Subtypen". Doctoral thesis, Universitätsbibliothek Leipzig, 2010. http://nbn-resolving.de/urn:nbn:de:bsz:15-qucosa-62204.
The aim of this doctor thesis was the electrophysiological characterization of the human 5-HT3A+E receptor which was recently cloned for the first time by NIESLER et al. (2003). Since the expression of this receptor subtype takes place exclusively in gastrointestinal tract, an influence on nausea and emesis is very likely. The question is if functional differences exist between homomeric 5-HT3A receptors and heteromeric 5-HT3A+B receptors, and whether different effects from emetic and antiemetic drugs can be detected at the molecular level. To assess the mechanisms and the interactions of a drug with the 5-HT3 receptor subtypes, knowledge of the biophysical characteristics and the pharmacological sensitivity of the 5-HT3 receptor subunit is required. The experiments were developed in-vitro on heterologous expressed receptors in HEK293-cells, whereat only the 5-HT3A subunit is able to form functional homopentameric receptors. The 5-HT3E and the 5-HT3B subunit can only be expressed on the cell surface and build functional heteropentameric receptors in combination with the 5-HT3A subunit. In the course of the investigations it became obvious that during transfection the 5-HT3E subunit and the 5-HT3B subunit are significantly lesser expressed than the 5-HT3A subunit. Using the patch-clamp technique in the excised-patch (outside-out) and whole-cell configuration it was possible to analyse the pharmacological and biophysical properties of the heteromeric 5-HT3A+E receptor compared with the homomeric 5-HT3A-receptor and the heteromeric 5-HT3A+B receptor. During the characterisation of the human 5-HT3A+E receptor subtype, the agonist concentration-response curve with the hillslope of 1,0 showed a significant flatter course than the graph of the 5-HT3A receptor subtype with a hillslope of 1,5. This indicates a diminished agonist binding-cooperativeness of the 5-HT3A+E receptor. No difference could be detected in the affinity to 5-HT, since the EC50 values of both receptor-subtypes were at the range of 7 µM. The biphasic course of the graph showed that by transfection of the heteromeric 5-HT3A+E receptor the homomeric 5-HT3A-receptor is expressed parallel. The same properties were described also for the 5-HT3A+B receptor (WALSTAB et al. 2008). Therefore it is difficult to distinguish the properties of a homomeric receptor by characterisation of a heteromeric receptor subtype. Furthermore, a faster desensitization of the heteromeric 5-HT3A+E-receptor could be demonstrated in comparison to homomeric 5-HT3A-receptor. Overall, the results described above indicate an increased sensitivity to the receptor for serotonin. As the 5-HT3A+E receptor is expressed exclusively in the gastro-intestinal tract, this could be an indication of involvement of this receptor in the mediation of emesis. During the pharmacological characterisation the partial 5-HT3 receptor agonist tryptamine, the full 5-HT3 receptor antagonist tropisetron as well as the partial 5-HT3 receptor antagonists metoclopramide, tubocurarin, mirtazapin and the cannabinoid receptor agonist anandamide, which has an emetic effect, were examined. The agonist concentration-response curve of metoclopramide (5-HT3A+E receptor: hillslope = -0,8; 5-HT3A receptor: hillslope = -1,2) and of mirtazapin (5-HT3A+E receptor: hillslope = -0,9; 5-HT3A receptor: hillslope = -1,3) showed a significant flatter course at the 5-HT3A+E receptor. Mirtazapin has an IC50 value of 8,4 nM at the 5-HT3A+E receptor in comparison to 25,4 nM at the 5-HT3A receptor. This significant higher potency of mirtazapin at the heteromeric 5-HT3 receptor subtype and the decreased binding-cooperativeness of mirtazapin and meteclopramide at the 5-HT3A+E receptor represent interesting approaches for an effective pharmacotherapy for gastrointestinal diseases. For the first time the results of this thesis showed the electrophysiological properties of the human 5-HT3A+E receptors and their interference by emetic and antiemetic drugs on the molecular level. Due to the decreased expression of 5-HT3E subunit, the goal for the future is to find an alternative way of transfection which increases the rate of yield for the 5-HT3A+E receptors
Wolf, Markus [Verfasser]. "Charakterisierung neuer Hypokaliämische Periodische Paralysen verursachender Mutationen in Domäne III des spannungsabhängigen Natriumkanals 1.4 mit Hilfe der Patch-clamp Technik / Markus Wolf". Ulm : Universität Ulm. Medizinische Fakultät, 2013. http://d-nb.info/1035700263/34.
Pippel, Anja [Verfasser], Fritz [Akademischer Betreuer] Markwardt, Milton Theodor [Akademischer Betreuer] Stubbs y Klaus [Akademischer Betreuer] Benndorf. "Struktur-Funktionsbeziehungen des humanen P2X7-Rezeptors Untersuchungen mittels Cysteinmutagenese und Patch-clamp-Technik / Anja Pippel ; Fritz Markwardt, Milton Theodor Stubbs, Klaus Benndorf". Halle, 2016. http://d-nb.info/1116950510/34.
Fidzinski, Pawel. "Elektrophysiologische Charakterisierung künstlicher Ionenkanäle in lebenden Zellen". Doctoral thesis, Humboldt-Universität zu Berlin, Medizinische Fakultät - Universitätsklinikum Charité, 2006. http://dx.doi.org/10.18452/15472.
Ion channels play a pivotal role for regular cell function. To better understand their structure and function, investigation of both natural and artificial ion channels is being performed to date. Investigation of artificial channels in living cells hides a big potential, however, little attention has been paid to this field so far. In this work, the effect of the artificial ion channels THF-gram, THF-gram-TBDPS and linked-gram-TBDPS on electrophysiological properties of bovine trabecular meshwork cells was investigated with the patch-clamp-technique. Following results were obtained: 1. Incorporation of all three compounds was successful, which was proven by increase of current density and cell depolarisation. 2. The cells survived after incorporation of THF-gram and THF-gram-TBDPS but not after linked-gram-TBDPS, which resulted in cell death at very low concentrations. 3. Larger outward currents were observed with THF-gram and, at a lower extent, with THF-gram-TBDPS. Linked-gram-TBDPS did not show such an asymmetry. 4. With Cs+ as charge carrier all three compunds showed a stronger increase of current density than under physiological conditions (Na+/K+). 5. The decribed results are a first step towards therapeutic application of artificial ion channels, however, further development towards higher selectivity and better control is as necessary as clarification of clinical feasibility.
Scherer, Claudia [Verfasser], Joachim [Akademischer Betreuer] Ehrlich y Rüdiger [Akademischer Betreuer] Hilker-Roggendorf. "Untersuchung des IK,PO und seiner zugrundeliegenden Untereinheiten in primären Zellkulturen atrialer Kardiomyozyten des Schweines mittels Patch-Clamp-Technik und molekularbiologischer Untersuchungen / Claudia Scherer. Gutachter: Joachim Ehrlich ; Rüdiger Hilker-Roggendorf". Frankfurt am Main : Univ.-Bibliothek Frankfurt am Main, 2015. http://d-nb.info/107936157X/34.
Kratzer, Stephan Alexander [Verfasser], Gerhard K. [Akademischer Betreuer] Rammes, Arthur [Akademischer Betreuer] Konnerth y Stefan [Akademischer Betreuer] Engelhardt. "Der Einfluss des Inhalationsanästhetikums Xenon auf die synaptische Transmission : Untersuchungen mit der Patch-Clamp-Technik in vitalen ZNS-Schnittpräparaten / Stephan Alexander Kratzer. Gutachter: Arthur Konnerth ; Stefan Engelhardt. Betreuer: Gerhard K. Rammes". München : Universitätsbibliothek der TU München, 2012. http://d-nb.info/102335070X/34.
Capítulos de libros sobre el tema "Patch-Clamp Techniken":
Vogel, Sebastian. "Die Patch-Clamp-Technik". En Zellbiologie 2, 37–39. Berlin, Heidelberg: Springer Berlin Heidelberg, 2017. http://dx.doi.org/10.1007/978-3-662-55070-0_10.
Eickhorn, R. "Messung des schnellen Natriumeinstroms mit der „Loose-patch-clamp“-Technik — Einfluß von Prajmalin". En Aspekte der medikamentösen Behandlung von Herzrhythmusstörungen, 25–31. Berlin, Heidelberg: Springer Berlin Heidelberg, 1989. http://dx.doi.org/10.1007/978-3-642-93403-2_3.