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Artículos de revistas sobre el tema "PHA productivity"

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Publicado: 22 de febrero de 2023
Última modificación: 23 de febrero de 2023

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1

Jin, Huanan, and Basil J. Nikolau. "Evaluating PHA Productivity of Bioengineered Rhodosprillum rubrum." PLoS ONE 9, no. 5 (2014): e96621. http://dx.doi.org/10.1371/journal.pone.0096621.

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2

Ruiz, Carolina, Shane T. Kenny, Ramesh Babu P, Meg Walsh, Tanja Narancic, and Kevin E. O’Connor. "High Cell Density Conversion of Hydrolysed Waste Cooking Oil Fatty Acids Into Medium Chain Length Polyhydroxyalkanoate Using Pseudomonas putida KT2440." Catalysts 9, no. 5 (2019): 468. http://dx.doi.org/10.3390/catal9050468.

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Waste cooking oil (WCO) is a major pollutant, primarily managed through incineration. The high cell density bioprocess developed here allows for better use of this valuable resource since it allows the conversion of WCO into biodegradable polymer polyhydroxyalkanoate (PHA). WCO was chemically hydrolysed to give rise to a mixture of fatty acids identical to the fatty acid composition of waste cooking oil. A feed strategy was developed to delay the stationary phase, and therefore achieve higher final biomass and biopolymer (PHA) productivity. In fed batch (pulse feeding) experiments Pseudomonas
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3

Blunt, Warren, David Levin, and Nazim Cicek. "Bioreactor Operating Strategies for Improved Polyhydroxyalkanoate (PHA) Productivity." Polymers 10, no. 11 (2018): 1197. http://dx.doi.org/10.3390/polym10111197.

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Microbial polyhydroxyalkanoates (PHAs) are promising biodegradable polymers that may alleviate some of the environmental burden of petroleum-derived polymers. The requirements for carbon substrates and energy for bioreactor operations are major factors contributing to the high production costs and environmental impact of PHAs. Improving the process productivity is an important aspect of cost reduction, which has been attempted using a variety of fed-batch, continuous, and semi-continuous bioreactor systems, with variable results. The purpose of this review is to summarize the bioreactor operat
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4

Maclean, Heather, Zhiyong Sun, Juliana Ramsay, and Bruce Ramsay. "Decaying exponential feeding of nonanoic acid for the production of medium-chain-length poly(3-hydroxyalkanoates) by Pseudomonas putida KT2440." Canadian Journal of Chemistry 86, no. 6 (2008): 564–69. http://dx.doi.org/10.1139/v08-062.

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Decaying exponential feeding strategies for the production of medium-chain-length poly(3-hydroxyalkanoates) by Pseudomonas putida KT2440 have been developed as a method of delaying oxygen limitation that is typically responsible for the termination of high cell density aerobic fermentations. Two feeding strategies were evaluated to control the biomass generation rate and consequently control the oxygen uptake rate of the cells. A linear decaying strategy allowed the production of 90 g L–1 dry biomass containing 65% PHA in 31 h with a cumulative productivity of 1.9 g L–1 h–1, while a quadratic
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5

Choi, Jong-il, Sang Yup Lee, and Kyuboem Han. "Cloning of the Alcaligenes latus Polyhydroxyalkanoate Biosynthesis Genes and Use of These Genes for Enhanced Production of Poly(3-hydroxybutyrate) in Escherichia coli." Applied and Environmental Microbiology 64, no. 12 (1998): 4897–903. http://dx.doi.org/10.1128/aem.64.12.4897-4903.1998.

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ABSTRACT Polyhydroxyalkanoates (PHAs) are microbial polyesters that can be used as completely biodegradable polymers, but the high production cost prevents their use in a wide range of applications. RecombinantEscherichia coli strains harboring the Ralstonia eutropha PHA biosynthesis genes have been reported to have several advantages as PHA producers compared with wild-type PHA-producing bacteria. However, the PHA productivity (amount of PHA produced per unit volume per unit time) obtained with these recombinantE. coli strains has been lower than that obtained with the wild-type bacterium Alc
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6

Takabatake, H., H. Satoh, T. Mino, and T. Matsuo. "Recovery of biodegradable plastics from activated sludge process." Water Science and Technology 42, no. 3-4 (2000): 351–56. http://dx.doi.org/10.2166/wst.2000.0402.

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In this research, PHA (polyhydroxyalkanoate) production system by activated sludge was studied. PHA behaves as carbon and energy storage material in bacteria. And PHA is a biodegradable plastic when extracted from bacteria. In this paper, the investigations from 3 aspects were reported; control of PHA composition, PHA production under coexistence of nitrogenous compounds, and influence of enrichment condition on PHA productivity. As results, it was possible to regulate PHA composition by utilizing acetate and propionate as carbon source and by regulating its composition. Nitrogenous compounds
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7

Chakraborty, Panchali, Kasiviswanathan Muthukumarappan, and William R. Gibbons. "PHA Productivity and Yield ofRalstonia eutrophaWhen Intermittently or Continuously Fed a Mixture of Short Chain Fatty Acids." Journal of Biomedicine and Biotechnology 2012 (2012): 1–8. http://dx.doi.org/10.1155/2012/506153.

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The research described in this present study was part of a larger effort focused on developing a dual substrate, dual fermentation process to produce Polyhydroxyalkanoate (PHA). The focus of this study was developing and optimizing a strategy for feeding a mixture of SCFAs (simulated ARF) and maximizing PHA production in a cost-effective way. Three different feeding strategies were examined in this study. The substrate evaluated in this study for the growth phase ofR. eutrophawas condensed corn solubles, a low-value byproduct of the dry-mill, corn ethanol industry. The culture was grown to hig
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8

Blunt, Warren, Christopher Dartiailh, Richard Sparling, Daniel J. Gapes, David B. Levin, and Nazim Cicek. "Development of High Cell Density Cultivation Strategies for Improved Medium Chain Length Polyhydroxyalkanoate Productivity Using Pseudomonas putida LS46." Bioengineering 6, no. 4 (2019): 89. http://dx.doi.org/10.3390/bioengineering6040089.

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High cell density (HCD) fed-batch cultures are widely perceived as a requisite for high-productivity polyhydroxyalkanoate (PHA) cultivation processes. In this work, a reactive pulse feed strategy (based on real-time CO2 or dissolved oxygen (DO) measurements as feedback variables) was used to control an oxygen-limited fed-batch process for improved productivity of medium chain length (mcl-) PHAs synthesized by Pseudomonas putida LS46. Despite the onset of oxygen limitation half-way through the process (14 h post inoculation), 28.8 ± 3.9 g L−1 total biomass (with PHA content up to 61 ± 8% cell d
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9

Carvalho, João M., Bruno C. Marreiros, and Maria A. M. Reis. "Polyhydroxyalkanoates Production by Mixed Microbial Culture under High Salinity." Sustainability 14, no. 3 (2022): 1346. http://dx.doi.org/10.3390/su14031346.

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The fishing industry produces vast amounts of saline organic side streams that require adequate treatment and disposal. The bioconversion of saline resources into value-added products, such as biodegradable polyhydroxyalkanoates (PHAs), has not yet been fully explored. This study investigated PHA production by mixed microbial cultures under 30 gNaCl/L, the highest NaCl concentration reported for the acclimatization of a PHA-accumulating mixed microbial culture (MMC). The operational conditions used during the culture-selection stage resulted in an enriched PHA-accumulating culture dominated by
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10

Palmeiro-Sánchez, Tania, José Luis Campos, and Anuska Mosquera-Corral. "Bioconversion of Organic Pollutants in Fish-Canning Wastewater into Volatile Fatty Acids and Polyhydroxyalkanoate." International Journal of Environmental Research and Public Health 18, no. 19 (2021): 10176. http://dx.doi.org/10.3390/ijerph181910176.

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The wastewater from the cookers of a tuna-canning plant was used as feedstock for the process. It was acidified in a continuous stirred tank reactor (CSTR) of 1.5 L to produce a mixture of volatile fatty acids (VFAs). The effluent contained 28.3 ± 8.7 g CODS/L and 25.0 ± 4.6 g CODVFA/L, 4.4 ± 1.6 g NH4+/L, and 10.9 ± 4.0 g Na+/L, which corresponds to about 28 g NaCl/L approximately. This was used to feed a PHA production system. The enriched MMC presented a capacity to accumulate PHAs from the fermented tuna wastewater. The maximum PHA content of the biomass in the fed-batch (8.35 wt% PHA) see
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11

Mifune, Jun, Satoshi Nakamura, and Toshiaki Fukui. "Targeted engineering of Cupriavidus necator chromosome for biosynthesis of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) from vegetable oil." Canadian Journal of Chemistry 86, no. 6 (2008): 621–27. http://dx.doi.org/10.1139/v08-047.

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Previous studies have demonstrated that heterologous expression of PHA synthase from Aeromonas caviae (PhaCAc), capable of accepting (R)-3-hydroxyacyl-CoA of C4–C7 as substrates, could confer the ability to PHA-negative mutant of Cupriavidus necator PHB-4 to synthesize poly((R)-3-hydroxybutyrate-co-(R)-3-hydroxyhexanoate) [P(3HB-co-3HHx)] from vegetable oils. The mutation point within pha operon in PHB-4 was determined to be a single nonsense mutation within the PHA synthase gene (phaCCn), suggesting the much lower β-ketothiolase and NADPH-dependent acetoacetyl-CoA reductase activities observe
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12

Mok, Pei-Shze, Jo-Ann Chuah, Nazalan Najimudin, Pauline-Woan-Ying Liew, Bor-Chyan Jong, and Kumar Sudesh. "In Vivo Characterization and Application of the PHA Synthase from Azotobacter vinelandii for the Biosynthesis of Polyhydroxyalkanoate Containing 4-Hydroxybutyrate." Polymers 13, no. 10 (2021): 1576. http://dx.doi.org/10.3390/polym13101576.

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Polyhydroxyalkanoate (PHA) is a biodegradable thermoplastic naturally synthesized by many microorganisms, and the PHA synthase (PhaC) is known to be the key enzyme involved in determining the material properties and monomer composition of the produced PHA. The ability to exploit widely distributed, commonly found soil microorganisms such as Azotobacter vinelandii to synthesize PHA containing the lipase-degradable 4-hydroxybutyrate (4HB) monomer will allow for convenient production of biocompatible and flexible PHA. Comparisons between the A. vinelandii wild type and mutant strains, with and wi
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13

Setiadi, Tjandra, Martha Aznury, Azis Trianto, and Adi Pancoro. "Production of polyhydroxyalkanoate (PHA) by Ralstonia eutropha JMP 134 with volatile fatty acids from palm oil mill effluent as precursors." Water Science and Technology 72, no. 11 (2015): 1889–95. http://dx.doi.org/10.2166/wst.2015.391.

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The highest volatile fatty acids (VFAs) concentration from palm oil mill effluent (POME) treated by anaerobic fermentation was achieved for a 1-day process when the main acids used were acetic, propionic and butyric acids. Polyhydroxyalkanoate (PHA) production with VFAs from POME as precursors in the fed-batch mode has advantages over batch mode, both in terms of its productivity and 3HV (3-hydroxyvalerate) composition in the produced polymer. With the fed batch, the productivity increased to 343% and contained more 3HV than those of the batch. The structures of the PHA were identified by diff
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14

Tu, Wei-Lin, Hsiao-Kai Chu, Chun-Mei Huang, Chia-Hsin Chen, Chung-Mao Ou, and Gia-Luen Guo. "Polyhydroxyalkanoate production by Cupriavidus necator with inedible rice." BioResources 17, no. 2 (2022): 2202–13. http://dx.doi.org/10.15376/biores.17.2.2202-2213.

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Because of their lower environmental impact, biodegradable polymers such as polyhydroxyalkanoates (PHAs) produced within cultured bacteria represent promising alternatives to petroleum-based plastics. PHA production in flasks yielded optimal results with a carbon-to-nitrogen ratio of 22. The 5-L scale experimental results revealed that when glucose was used as the carbon source, Cupriavidus necator could produce 4.74 g/L PHA with 77.6% of PHA content in the microorganism 72 h after the initiation of the experiment. When the hydrolysis liquid from inedible rice was used as the carbon source, th
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15

Rodiño, Ana P., Manuel Riveiro, and Antonio M. De Ron. "Implications of the Symbiotic Nitrogen Fixation in Common Bean under Seasonal Water Stress." Agronomy 11, no. 1 (2020): 70. http://dx.doi.org/10.3390/agronomy11010070.

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The objective of this research was to study the performance of 10 common bean genotypes under water deficit stress and how it affects to their symbiotic relationship with 10 Rhizobium strains in both greenhouse and field conditions. PHA-0471, a small seeded genotype had the best yield under irrigation and under water stress. Other genotypes with tolerance to drought were the large-seeded PHA-0432 and PHA-0683. In the Rhizobium inoculation tests it was observed that the increase of dry nodular weight produced less seed yield in beans. PHA-0683 genotype presented a great uniformity on nodule siz
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16

Cabrera, Francisco, Álvaro Torres, José Luis Campos, and David Jeison. "Effect of Operational Conditions on the Behaviour and Associated Costs of Mixed Microbial Cultures for PHA Production." Polymers 11, no. 2 (2019): 191. http://dx.doi.org/10.3390/polym11020191.

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Massive production and disposal of petrochemical derived plastics represent relevant environmental problems. Polyhydroxyalkanoates (PHA) are a renewable alternative that can even be produced from wastes. The production of PHA from acetate using mixed microbial cultures was studied. The effect of two key operational conditions was evaluated, i.e., substrate concentration and cycle length. The effects of these factors on several responses were studied using a surface response methodology. Several reactors were operated under selected conditions for at least 10 solids retention times to ensure st
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17

Panaksri, Anuchan, and Nuttapol Tanadchangsaeng. "Evaluation of 3D-Printing Scaffold Fabrication on Biosynthetic Medium-Chain-Length Polyhydroxyalkanoate Terpolyester as Biomaterial-Ink." Polymers 13, no. 14 (2021): 2222. http://dx.doi.org/10.3390/polym13142222.

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Currently, the selection of materials for tissue engineering scaffolds is still limited because some tissues require flexible and compatible materials with human cells. Medium-chain-length polyhydroxyalkanoate (MCL-PHA) synthesized in microorganisms is an interesting polymer for use in this area and has elastomeric properties compatible with the human body. MCL-PHAs are elastomers with biodegradability and cellular compatibility, making them an attractive material for fabricating soft tissue that requires high elasticity. In this research, MCL-PHA was produced by fed-batch fermentation that Ps
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18

Chinwetkitvanich, S., C. W. Randall, and T. Panswad. "Effects of phosphorus limitation and temperature on PHA production in activated sludge." Water Science and Technology 50, no. 8 (2004): 135–43. http://dx.doi.org/10.2166/wst.2004.0507.

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The study was designed to investigate the effects of temperature and phosphorus limitation on polyhydroxyalkanoate (PHA) production and storage by activated sludge biomass. The two-stage operation approach, i.e. a growth phase followed by a nutrient limitation phase, was applied to induce PHA accumulation. The pre-selected temperatures of 10, 20 and 30°C were investigated under phosphorus limitation conditions using three four-litre fully aerobic SBR systems operated at an SRT of 10 days with cycle time and HRT of 6 and 10 hours. PHA production was greater in the 10°C system than in the 20°C a
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19

Oliveira, Catarina, Marisa Silva, Carlos E. Silva, Gilda Carvalho, and Maria A. M. Reis. "Assessment of Protein-Rich Cheese Whey Waste Stream as a Nutrients Source for Low-Cost Mixed Microbial PHA Production." Applied Sciences 8, no. 10 (2018): 1817. http://dx.doi.org/10.3390/app8101817.

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The critical step in the mixed microbial cultures (MMC) polyhydroxyalkanoates (PHA) production process is the selection of an MMC enriched in an efficient PHA-accumulating organism, usually requiring growth-nutrients supplementation. This study aimed at assessing cheese whey (CW) proteins as a source of nitrogen for PHA-producing MMC, thus eliminating or reducing the need for nutrients supplementation. The selection reactor, operated with fermented CW, under a feast–famine regime, was initially supplemented with ammonia–nitrogen in a C/N ratio of 100/15 (Cmol/Nmmol), which was gradually reduce
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20

Ahn, Woo Suk, Si Jae Park, and Sang Yup Lee. "Production of Poly(3-Hydroxybutyrate) by Fed-Batch Culture of Recombinant Escherichia coliwith a Highly Concentrated Whey Solution." Applied and Environmental Microbiology 66, no. 8 (2000): 3624–27. http://dx.doi.org/10.1128/aem.66.8.3624-3627.2000.

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ABSTRACT Fermentation strategies for the production of poly(3-hydroxybutyrate) (PHB) from whey by recombinantEscherichia coli strain CGSC 4401 harboring theAlcaligenes latus polyhydroxyalkanoate (PHA) biosynthesis genes were developed. The pH-stat fed-batch cultures of E. coli CGSC 4401 harboring pJC4, a stable plasmid containing theA. latus PHA biosynthesis genes, were carried out with a concentrated whey solution containing 280 g of lactose equivalent per liter. Final cell and PHB concentrations of 119.5 and 96.2 g/liter, respectively, were obtained in 37.5 h, which resulted in PHB productiv
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Lascu, Irina, Ana Maria Tănase, Piotr Jablonski, et al. "Revealing the Phenotypic and Genomic Background for PHA Production from Rapeseed-Biodiesel Crude Glycerol Using Photobacterium ganghwense C2.2." International Journal of Molecular Sciences 23, no. 22 (2022): 13754. http://dx.doi.org/10.3390/ijms232213754.

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Polyhydroxyalkanoates (PHA) are promising biodegradable and biocompatible bioplastics, and extensive knowledge of the employed bacterial strain’s metabolic capabilities is necessary in choosing economically feasible production conditions. This study aimed to create an in-depth view of the utilization of Photobacterium ganghwense C2.2 for PHA production by linking a wide array of characterization methods: metabolic pathway annotation from the strain’s complete genome, high-throughput phenotypic tests, and biomass analyses through plate-based assays and flask and bioreactor cultivations. We conf
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22

Szacherska, Karolina, Krzysztof Moraczewski, Sylwester Czaplicki, Piotr Oleskowicz-Popiel, and Justyna Mozejko-Ciesielska. "Conversion of Short and Medium Chain Fatty Acids into Novel Polyhydroxyalkanoates Copolymers by Aeromonas sp. AC_01." Materials 15, no. 13 (2022): 4482. http://dx.doi.org/10.3390/ma15134482.

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Polyhydroxyalkanoates (PHAs) production by Aeromonas sp. AC_01 was investigated using synthetic and waste derived short and medium chain fatty acids (SMCFAs). The obtained results revealed that the analyzed bacterial strain was able to grow and synthesize PHAs using SMCFAs. The highest PHA productivity was observed in the cultivation supplemented with a mixture of acetic acid and butyric acid (3.89 mg/L·h). Furthermore, SMCFAs-rich stream, derived from acidogenic mixed culture fermentation of acid whey, was found to be less beneficial for PHA productivity than its synthetic mixture, however th
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Kamravamanesh, Donya, Maximilian Lackner, and Christoph Herwig. "Bioprocess Engineering Aspects of Sustainable Polyhydroxyalkanoate Production in Cyanobacteria." Bioengineering 5, no. 4 (2018): 111. http://dx.doi.org/10.3390/bioengineering5040111.

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Polyhydroxyalkanoates (PHAs) are a group of biopolymers produced in various microorganisms as carbon and energy reserve when the main nutrient, necessary for growth, is limited. PHAs are attractive substitutes for conventional petrochemical plastics, as they possess similar material properties, along with biocompatibility and complete biodegradability. The use of PHAs is restricted, mainly due to the high production costs associated with the carbon source used for bacterial fermentation. Cyanobacteria can accumulate PHAs under photoautotrophic growth conditions using CO2 and sunlight. However,
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Bosco, Francesca, Simona Cirrincione, Riccardo Carletto, et al. "PHA Production from Cheese Whey and “Scotta”: Comparison between a Consortium and a Pure Culture of Leuconostoc mesenteroides." Microorganisms 9, no. 12 (2021): 2426. http://dx.doi.org/10.3390/microorganisms9122426.

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It is urgent to expand the market of biodegradable alternatives to oil-derived plastics owing to (i) increasingly limited oil availability/accessibility, and (ii) the dramatic impact of traditional plastics on aquatic life, the food chain, all Earth ecosystems, and ultimately, human health. Polyhydroxyalkanoates (PHAs) are promising biodegradable polymers that can be obtained through microbial fermentation of agro-industrial byproducts, e.g., milk and cheese whey. Here, the PHA-accumulating efficiency of a mixed microbial culture (MMC, derived from activated sludges) grown on dairy byproducts
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Vermeer, Chris M., Larissa J. Bons, and Robbert Kleerebezem. "Production of a newly discovered PHA family member with an isobutyrate-fed enrichment culture." Applied Microbiology and Biotechnology 106, no. 2 (2022): 605–18. http://dx.doi.org/10.1007/s00253-021-11742-9.

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Abstract Using microbial enrichment cultures for the production of waste-derived polyhydroxyalkanoates (PHAs) is a promising technology to recover secondary resources. Volatile fatty acids (VFAs) form the preferred substrate for PHA production. Isobutyrate is a VFA appearing in multiple waste valorization routes, such as anaerobic fermentation, chain elongation, and microbial electrosynthesis, but has never been assessed individually on its PHA production potential. This research investigates isobutyrate as sole carbon source for a microbial enrichment culture in comparison to its structural i
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Goto, Saki, Yuki Miyahara, Seiichi Taguchi, Takeharu Tsuge, and Ayaka Hiroe. "Enhanced Production of (R)-3-Hydroxybutyrate Oligomers by Coexpression of Molecular Chaperones in Recombinant Escherichia coli Harboring a Polyhydroxyalkanoate Synthase Derived from Bacillus cereus YB-4." Microorganisms 10, no. 2 (2022): 458. http://dx.doi.org/10.3390/microorganisms10020458.

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The biodegradable polyester poly-(R)-3-hydroxybutyrate [P(3HB)] is synthesized by a polymerizing enzyme called polyhydroxyalkanoate (PHA) synthase and accumulates in a wide variety of bacterial cells. Recently, we demonstrated the secretory production of a (R)-3HB oligomer (3HBO), a low-molecular-weight P(3HB), by using recombinant Escherichia coli expressing PHA synthases. The 3HBO has potential value as an antibacterial substance and as a building block for various polymers. In this study, to construct an efficient 3HBO production system, the coexpression of molecular chaperones and a PHA sy
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Hermann-Krauss, Carmen, Martin Koller, Alexander Muhr, Hubert Fasl, Franz Stelzer, and Gerhart Braunegg. "Archaeal Production of Polyhydroxyalkanoate (PHA) Co- and Terpolyesters from Biodiesel Industry-Derived By-Products." Archaea 2013 (2013): 1–10. http://dx.doi.org/10.1155/2013/129268.

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The archaeonHaloferax mediterraneiwas selected for production of PHA co- and terpolyesters using inexpensive crude glycerol phase (CGP) from biodiesel production as carbon source. CGP was assessed by comparison with the application of pure glycerol. Applying pure glycerol, a copolyester with a molar fraction of 3-hydroxybutyrate (3HB) of 0.90 mol/mol and 3-hydroxyvalerate (3HV) of 0.10 mol/mol, was produced at a volumetric productivity of 0.12 g/Lh and an intracellular PHA content of 75.4 wt.-% in the sum of biomass protein plus PHA. Application of CGP resulted in the same polyester compositio
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Werker, Alan, Laura Lorini, Marianna Villano, Francesco Valentino, and Mauro Majone. "Modelling Mixed Microbial Culture Polyhydroxyalkanoate Accumulation Bioprocess towards Novel Methods for Polymer Production Using Dilute Volatile Fatty Acid Rich Feedstocks." Bioengineering 9, no. 3 (2022): 125. http://dx.doi.org/10.3390/bioengineering9030125.

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Volatile fatty acid (VFA) rich streams from fermentation of organic residuals and wastewater are suitable feedstocks for mixed microbial culture (MMC) Polyhydroxyalkanoate (PHA) production. However, many such streams have low total VFA concentration (1–10 gCOD/L). PHA accumulation requires a flow-through bioprocess if the VFAs are not concentrated. A flow through bioprocess must balance goals of productivity (highest possible influent flow rates) with goals of substrate utilization efficiency (lowest possible effluent VFA concentration). Towards these goals, dynamics of upshift and downshift r
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Abdelmalek, Fady, Alexander Steinbüchel, and Marian Rofeal. "The Hyperproduction of Polyhydroxybutyrate Using Bacillus mycoides ICRI89 through Enzymatic Hydrolysis of Affordable Cardboard." Polymers 14, no. 14 (2022): 2810. http://dx.doi.org/10.3390/polym14142810.

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Bioplastics are contemplated as remarkable substitutes for conventional plastics to accommodate green technological advancements. However, their industrial production has not been fully implemented owing to the cost of carbon resources. From another perspective, valorizing different paper mill wastes has become a prominent research topic. These materials may serve as an affording sustainable feedstock for bioplastic production. Adjustment of cardboard waste hydrolysate as suitable fermentation media for production of bacterial polyhydroxyalkanoates (PHAs) has been investigated. Cardboard sampl
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Calero, Rolando, and Manuel Martínez. "Effect of the solid retention time in the obtention of polyhydroxyalkanoates." F1000Research 10 (August 27, 2021): 864. http://dx.doi.org/10.12688/f1000research.28852.1.

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Background: The effect of solid retention time (SRT) over cheese whey substrates in a fermentation process drives changes in the composition of polyhydroxyalkanoates (PHAs) obtained. Volatile fatty acids produced in the first step of an anaerobic sequencing batch reactor were used as substrates to produce PHA using mixed microbial cultures under aerobic dynamic feeding conditions. Methods: Analytical methods were used for the standard analysis of parameters of interest including measuring the amount of ammonium and phosphate, chemical oxygen demand, among others. Results: The SRT increasing fr
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Berezina, Nathalie. "Novel approach for productivity enhancement of polyhydroxyalkanoates (PHA) production by Cupriavidus necator DSM 545." New Biotechnology 30, no. 2 (2013): 192–95. http://dx.doi.org/10.1016/j.nbt.2012.05.002.

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Koller, Martin, Denis Vadlja, Gerhart Braunegg, Aid Atlić, and Predrag Horvat. "Formal- and high-structured kinetic process modelling and footprint area analysis of binary imaged cells: Tools to understand and optimize multistage-continuous PHA biosynthesis." EuroBiotech Journal 1, no. 3 (2017): 203–11. http://dx.doi.org/10.24190/issn2564-615x/2017/03.01.

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Abstract Competitive polyhydroxyalkanoate (PHAs) production requires progress in microbial strain performance, feedstock selection, downstream processing, and more importantly according to the process design with process kinetics of the microbial growth phase and the phase of product formation. The multistage continuous production in a bioreactor cascade was described for the first time in a continuously operated, flexible five-stage bioreactor cascade that mimics the characteristics involved in the engineering process of tubular plug flow reactors. This process was developed and used for Cupr
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Melanie, Susiana, and James Winterburn. "Productivity of Marine Archaea Haloferax mediterranei Culture in Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) Production with Yeast Extract as Nutrient Source." Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology 12, no. 3 (2017): 117. http://dx.doi.org/10.15578/squalen.v12i3.318.

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Currently, most of household goods are made from polymer or plastic. The polymer manufacturing industry has been relying on fossil fuel-based raw materials which is non-biodegradable. Plastics derived from these non-biodegradable sources will be difficult to degrade in nature, which in turn will pollute the environment and harm the ecosystem. One way to reduce the ecosystem damages caused by plastic made from petroleum-based plastic is to use biodegradable materials as plastic industry raw materials, such as Polyhydroxyalkanoates (PHA). Poly (3-hydroxybutyrate-co-3-hydroxyvalerate) or PHBV is
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34

Tanadchangsaeng, Nuttapol, and Sittiruk Roytrakul. "Proteomic Examination for Gluconeogenesis Pathway-Shift during Polyhydroxyalkanoate Formation in Cupriavidus necator Grown on Glycerol." Bioengineering 7, no. 4 (2020): 154. http://dx.doi.org/10.3390/bioengineering7040154.

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Because of availability and inexpensiveness, glycerol can be considered as a suitable raw material for polyhydroxyalkanoate (PHA) production with bacterial fermentation. Nevertheless, compared to the production of glucose as a raw precursor, PHA produced from glycerol by Cupriavidus necator was found to produce lower PHA with low bacterial growth rates. According to our study, C. necator was able to synthesize glucose-like intermediates from glycerol via gluconeogenesis. This resulted in a decrease of the cell dry weight and the yield of PHA polymers, especially in the active cell growth phase
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35

Albuquerque, M. G. E., V. Martino, E. Pollet, L. Avérous, and M. A. M. Reis. "Mixed culture polyhydroxyalkanoate (PHA) production from volatile fatty acid (VFA)-rich streams: Effect of substrate composition and feeding regime on PHA productivity, composition and properties." Journal of Biotechnology 151, no. 1 (2011): 66–76. http://dx.doi.org/10.1016/j.jbiotec.2010.10.070.

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36

Koller, Martin. "Switching from petro-plastics to microbial polyhydroxyalkanoates (PHA): the biotechnological escape route of choice out of the plastic predicament?" EuroBiotech Journal 3, no. 1 (2019): 32–44. http://dx.doi.org/10.2478/ebtj-2019-0004.

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Abstract The benefit of biodegradable “green plastics” over established synthetic plastics from petro-chemistry, namely their complete degradation and safe disposal, makes them attractive for use in various fields, including agriculture, food packaging, and the biomedical and pharmaceutical sector. In this context, microbial polyhydroxyalkanoates (PHA) are auspicious biodegradable plastic-like polyesters that are considered to exert less environmental burden if compared to polymers derived from fossil resources. The question of environmental and economic superiority of bio-plastics has inspire
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37

Gutschmann, Björn, Thomas Schiewe, Manon T. H. Weiske, Peter Neubauer, Roland Hass, and Sebastian L. Riedel. "In-Line Monitoring of Polyhydroxyalkanoate (PHA) Production during High-Cell-Density Plant Oil Cultivations Using Photon Density Wave Spectroscopy." Bioengineering 6, no. 3 (2019): 85. http://dx.doi.org/10.3390/bioengineering6030085.

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Polyhydroxyalkanoates (PHAs) are biodegradable plastic-like materials with versatile properties. Plant oils are excellent carbon sources for a cost-effective PHA production, due to their high carbon content, large availability, and comparatively low prices. Additionally, efficient process development and control is required for competitive PHA production, which can be facilitated by on-line or in-line monitoring devices. To this end, we have evaluated photon density wave (PDW) spectroscopy as a new process analytical technology for Ralstonia eutropha (Cupriavidus necator) H16 plant oil cultiva
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38

Botturi, Alice, Federico Battista, Marco Andreolli, et al. "Polyhydroxyalkanoated-Rich Microbial Cells from Bio-Based Volatile Fatty Acids as Potential Ingredient for Aquaculture Feed." Energies 14, no. 1 (2020): 38. http://dx.doi.org/10.3390/en14010038.

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In this study, the production of polyhydroxyalkanoated PHA-rich microbial biomass as a novel feed additive in aquaculture was investigated at a lab-scale. Bio-based volatile fatty acids (VFAs), obtained from the acidogenic fermentation of agricultural residues in existing anaerobic digestion plants, were used as carbon and energy to cultivate the PHA-rich microbial biomass. The experimental activities were carried out using Thauera sp. Sel9 as pure strain, which was grown in a continuous stirred-tank reactor (CSTR) operated at three different hydraulic retention times (HRT). The highest produc
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39

Md Din, M. F., Z. Ujang, M. C. M. van Loosdrecht, A. Ahmad, and M. F. Sairan. "Optimization of nitrogen and phosphorus limitation for better biodegradable plastic production and organic removal using single fed-batch mixed cultures and renewable resources." Water Science and Technology 53, no. 6 (2006): 15–20. http://dx.doi.org/10.2166/wst.2006.164.

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The process for the production of biodegradable plastic material (polyhydroxyalkanoates, PHAs) from microbial cells by mixed-bacterial cultivation using readily available waste (renewable resources) is the main consideration nowadays. These observations have shown impressive results typically under high carbon fraction, COD/N and COD/P (usually described as nutrient-limiting conditions) and warmest temperature (moderate condition). Therefore, the aim of this work is predominantly to select mixed cultures under high storage responded by cultivation on a substrate – non limited in a single batch
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40

Oehmen, Adrian, Fátima V. Pinto, Vera Silva, Maria G. E. Albuquerque, and Maria A. M. Reis. "The impact of pH control on the volumetric productivity of mixed culture PHA production from fermented molasses." Engineering in Life Sciences 14, no. 2 (2013): 143–52. http://dx.doi.org/10.1002/elsc.201200220.

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41

Cabrera, Francisco, Álvaro Torres-Aravena, Fernanda Pinto-Ibieta, José Luis Campos, and David Jeison. "On-Line Control of Feast/Famine Cycles to Improve PHB Accumulation during Cultivation of Mixed Microbial Cultures in Sequential Batch Reactors." International Journal of Environmental Research and Public Health 18, no. 23 (2021): 12611. http://dx.doi.org/10.3390/ijerph182312611.

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Production of polyhydroxyalkanoates (PHA) has generated great interest as building blocks for bioplastic production. Their production using mixed microbial cultures represents an interesting alternative, since it enables the use of organic wastes as a carbon source. Feast/famine strategy is a common way to promote selection of microorganisms with PHA accumulation capacity. However, when using waste sources, changes in substrate concentration are expected, that may affect performance and efficiency of the process. This study showed how the dissolved oxygen level can be used for online control o
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42

Dhakal, Nirpesh, and Bishnu Acharya. "Syngas Fermentation for the Production of Bio-Based Polymers: A Review." Polymers 13, no. 22 (2021): 3917. http://dx.doi.org/10.3390/polym13223917.

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Increasing environmental awareness among the general public and legislators has driven this modern era to seek alternatives to fossil-derived products such as fuel and plastics. Addressing environmental issues through bio-based products driven from microbial fermentation of synthetic gas (syngas) could be a future endeavor, as this could result in both fuel and plastic in the form of bioethanol and polyhydroxyalkanoates (PHA). Abundant availability in the form of cellulosic, lignocellulosic, and other organic and inorganic wastes presents syngas catalysis as an interesting topic for commercial
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43

Sahilatua, Rivaldo W., Feky R. Mantiri, and Marhaenus J. Rumondor. "KAJIAN ETHYLENE TRIPLE RESPONSE TERHADAP KECAMBAH BEBERAPA VARIETAS KACANG BUNCIS (Phaseolus vulgaris L.)." PHARMACON 8, no. 3 (2019): 734. http://dx.doi.org/10.35799/pha.8.2019.29399.

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ABSTRACTOne of the appropriate cultivation techniques to increase land productivity, is intercropping cultivation. Intercropping is causing the plant to grow in shelter. This sheltered situation can increase ethylene production, giving rise to a triple response (inhibits stem lengthening, thickening of the stem, and the appearance of the habit of making hooks that cause the stem to grow horizontally or horizontally) that could affect plant growth and development. The aim of this study was to look for varieties of snaps (Phaseolus vulgaris) that were less sensitive to the increasing of ethylene
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44

Możejko-Ciesielska, Justyna, and Tomasz Pokoj. "Exploring nutrient limitation for polyhydroxyalkanoates synthesis by newly isolated strains ofAeromonassp. using biodiesel-derived glycerol as a substrate." PeerJ 6 (October 23, 2018): e5838. http://dx.doi.org/10.7717/peerj.5838.

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Aeromonasspp. strains isolated from activated sludge in a municipal wastewater treatment plant were found to be able to synthesize polyhydroxyalkanoates (PHA) utilizing pure and crude glycerol. The 16S rRNA gene sequence of the isolates exhibited similarity toAeromonas hydrophila,A. aquatica, andA. salmonicida. Our results confirmed that the adequate supply of nitrogen and phosphorus during culture in 250-ml shake flasks did not stimulate the synthesis of PHAs. The results indicate that the PHA content of cells was higher under a phosphorus-limiting environment compared to nitrogen starvation.
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45

Blunt, Warren, Marc Gaugler, Christophe Collet, et al. "Rheological Behavior of High Cell Density Pseudomonas putida LS46 Cultures during Production of Medium Chain Length Polyhydroxyalkanoate (PHA) Polymers." Bioengineering 6, no. 4 (2019): 93. http://dx.doi.org/10.3390/bioengineering6040093.

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The rheology of high-cell density (HCD) cultures is an important parameter for its impact on mixing and sparging, process scale-up, and downstream unit operations in bioprocess development. In this work, time-dependent rheological properties of HCD Pseudomonas putida LS46 cultures were monitored for microbial polyhydroxyalkanoate (PHA) production. As the cell density of the fed-batch cultivation increased (0 to 25 g·L−1 cell dry mass, CDM), the apparent viscosity increased nearly nine-fold throughout the fed-batch process. The medium behaved as a nearly Newtonian fluid at lower cell densities,
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46

Huu Phong, Truong, Dang Minh Khuong, Duong Van Hop, and Doan Van Thuoc. "DIFFERENT FRUCTOSE FEEDING STRATEGIES FOR POLY(3-HYDROXYBUTYRATE) PRODUCTION BY Yangia sp. ND199." Vietnam Journal of Science and Technology 55, no. 2 (2017): 195. http://dx.doi.org/10.15625/0866-708x/55/2/8677.

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Yangia sp. ND199 is a halophilic bacterium isolated from mangrove soil sample. This strain was able to produce polyhydroxyalkanoate (PHA) from different carbon sources. Only homopolymer poly(3-hydroxybutyrate) (PHB) was synthesized when fructose was used as carbon source. The bacterium can accumulate high PHB content during exponential phase. Maximum cell dry weight (CDW) of 7.8 g/l and PHB content of 49 wt% were obtained after 27 h of cultivation in batch fermentation. High CDW and PHB content were achieved by using fed-batch fermentation with different fructose feeding strategies. The highes
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47

Melanie, Susiana, and James Winterburn. "Production of Poly(3-Hydroxybutyrate-Co-3-Hydroxyvalerate) by Marine Archaea Haloferax mediterranei Dsm 1411 with Yeast Extract As Nutrient Source." Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology 12, no. 3 (2017): 117. http://dx.doi.org/10.15578/squalen.v12i3.314.

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In many countries, most of household goods are made from polymer or plastic. The polymer manufacturing industry has been relying on fossil fuel-based raw materials which are non-biodegradable. Plastics derived from these non-biodegradable sources will be difficult to degrade in nature, which in turn will pollute the environment and harm the ecosystem. One way to reduce the ecosystem damages caused by petroleum-based plastic is by using biodegradable materials for plastic industry, such as Polyhydroxyalkanoates (PHA). Poly (3-hydroxybutyrate-co-3-hydroxyvalerate) or PHBV is an example of PHA th
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48

Nikel, Pablo I., Alejandra de Almeida, Evelia C. Melillo, Miguel A. Galvagno, and M. Julia Pettinari. "New Recombinant Escherichia coli Strain Tailored for the Production of Poly(3-Hydroxybutyrate) from Agroindustrial By-Products." Applied and Environmental Microbiology 72, no. 6 (2006): 3949–54. http://dx.doi.org/10.1128/aem.00044-06.

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ABSTRACT A recombinant E. coli strain (K24K) was constructed and evaluated for poly(3-hydroxybutyrate) (PHB) production from whey and corn steep liquor as main carbon and nitrogen sources. This strain bears the pha biosynthetic genes from Azotobacter sp. strain FA8 expressed from a T5 promoter under the control of the lactose operator. K24K does not produce the lactose repressor, ensuring constitutive expression of genes involved in lactose transport and utilization. PHB was efficiently produced by the recombinant strain grown aerobically in fed-batch cultures in a laboratory scale bioreactor
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49

Abbas, Ahmed O., Abdulaziz A. Alaqil, Hossam S. El-Beltagi, Hanaa K. Abd El-Atty, and Nancy N. Kamel. "Modulating Laying Hens Productivity and Immune Performance in Response to Oxidative Stress Induced by E. coli Challenge Using Dietary Propolis Supplementation." Antioxidants 9, no. 9 (2020): 893. http://dx.doi.org/10.3390/antiox9090893.

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Propolis (PR) is a resin product of bee colonies that has rich bioactive antioxidant and bactericidal compounds. Endotoxin, a byproduct of bacterial growth, is reported to cause progressive induction of endogenous oxidative stress and has negative impacts on individual health and wellbeing. Hereby, we investigated the ability of PR to alleviate the oxidative stress and immunosuppression imposed by avian pathogenic Escherichia coli using laying hen as a based model. In this study, PR was dietary supplemented to hens for 4 weeks at a concentration of 0.1%. At the beginning of the 4th week of the
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50

Smith, Hunter Jackson. "A Burden and Prevalence Analysis of Chronic Pain by Distinct Case Definitions among Active Duty U.S. Military Service Members, 2018." Pain Physician 5;23, no. 9;5 (2020): E429—E439. http://dx.doi.org/10.36076/ppj.2020/23/e429.

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Background: Chronic pain is a growing problem in the military, and the methods by which we have to perform epidemiologic surveillance are insufficient. It represents both a public health and military readiness concern, as those who suffer from it experience adverse impacts on work productivity, physiological health, and quality of life. Objectives: This study was designed to assess the prevalence of chronic pain among active component military service members utilizing 2 distinct, published case definitions. It sought to describe the demographics and military characteristics of those receiving
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