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1
Lester, Larry G. "Parental satisfaction with phycho-educational consultation". Huntington, WV : [Marshall University Libraries], 2006. http://www.marshall.edu/etd/descript.asp?ref=705.
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White, Richard Lloyd. "Nomos and physis, callicles and Euripides' Cyclops". Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1997. http://www.collectionscanada.ca/obj/s4/f2/dsk3/ftp04/mq24943.pdf.
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Patzer, Harald. "Physis : Grundlegung zu einer Geschichte des Wortes /". Stuttgart : F. Steiner, 1993. http://catalogue.bnf.fr/ark:/12148/cb370179779.
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Jarvis, Stephen C. "Phytal meiofauna as biomonitors of environmental water quality". Thesis, Bangor University, 1994. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.283173.
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Stahlman, Michael McCann Laura. "Phytase anatomy of an invisible win-win technology /". Diss., Columbia, Mo. : University of Missouri--Columbia, 2009. http://hdl.handle.net/10355/6474.
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Title from PDF of title page (University of Missouri--Columbia, viewed on Feb 18, 2010). The entire thesis text is included in the research.pdf file; the official abstract appears in the short.pdf file; a non-technical public abstract appears in the public.pdf file. Thesis advisor: Dr. Laura McCann. Includes bibliographical references.
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Yong, Kwon-Joong. "Wheat bran phytase : distribution, purification and biochemical characterization /". Search for this dissertation online, 2004. http://wwwlib.umi.com/cr/ksu/main.
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Faba, Rodriguez Raquel. "Structure-function studies of a purple acid phytase". Thesis, University of East Anglia, 2018. https://ueaeprints.uea.ac.uk/69554/.
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The enzymatic cleavage of phosphate monoesters of myo-inositol hexakisphosphate (InsP6) or phytate is the property of a group of enzymes collectively known as phytases. These enzymes adopt a variety of protein folds and utilise a number of different reaction mechanisms and may be classified accordingly. Among these, the purple acid phytases (PAPhy), a subclass of the purple acid phosphatases (PAP), are the least well characterised. The aim of this thesis is a biochemical and structural characterisation of cereal PAPhy with the additional purpose of the identification of structural features that distinguish PAPhy from PAP. In this project, the partial enzymatic deglycosylation of a recombinant PAPhy from wheat yielded high quality crystals that allowed the solution of the high-resolution X-ray crystallographic structure of the first PAPhy, with inorganic phosphate bound in different poses and in complex with the inhibitor myo-inositol hexakissulfate. Molecular dynamics simulations of the enzyme-substrate complex allowed the identification of key protein-substrate interactions, leading to the proposal of six phytate specificity pockets for the wheat PAPhy isoform b2 (TaPAPhy_b2). A characterisation of TaPAPhy_b2 allowed the estimation of its kinetic parameters, revealed optimum phytase activity at pH 5.5 and 37°C, with denaturation and subsequent inactivation over 50°C, and the determination of the D-4/6-phosphate as preferred initiation site of InsP6 hydrolysis. A conservation of the pathway of phytate hydrolysis identified in TaPAPhy_b2 was observed in other cereal PAPhy, while the soybean PAPhy displayed higher positional promiscuity. Structure-function relationships of TaPAPhy_b2 were elucidated by site-directed mutagenesis and mutant characterisation alongside the wild type enzyme. Two amino acid residues critical for phytase activity were identified, His229 and Lys410, while a third, Lys348, was shown to influence substrate affinity more subtly. The work described in this thesis provides novel insights into the structure and phytase activity of the purple acid phytases.
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Kleist, Sophia. "Optimierung eines fermentativen Verfahrens zur Herstellung einer bakteriellen Phytase". [S.l. : s.n.], 2002. http://deposit.ddb.de/cgi-bin/dokserv?idn=965755134.
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Yi, Zhixiong. "Supplementing microbial phytase to diets for swine and poultry". Diss., Virginia Tech, 1995. http://hdl.handle.net/10919/40217.
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Dietel, Kristin. "Expression bakterieller Phytasen in Pflanzen". Doctoral thesis, Humboldt-Universität zu Berlin, Mathematisch-Naturwissenschaftliche Fakultät I, 2010. http://dx.doi.org/10.18452/16156.
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Die Verfügbarkeit des Makroelementes Phosphor ist für Lebewesen eingeschränkt. Besonders bei der Pflanzenproduktion und der Tierernährung spielt die Phosphorverfügbarkeit eine wichtige Rolle bei der ökonomischen Nutzung der Ressourcen. In den Fokus der Wissenschaft zur Lösung des Phosphorproblems gerieten die Phytasen, da monogastrische Tiere nicht in der Lage sind das in den Pflanzensamen gespeicherte Phytat zu nutzen. Die gentechnische Veränderung von Pflanzen stellt eine effiziente Möglichkeit zur Produktion von phosphatfreisetzenden Enzymen, zur Erhöhung der Biomasseproduktion und zur Veränderung der Inhaltstoffe dar. In dieser Arbeit wurden erfolgreich transgene Pflanzen der Arten Nicotiana tabacum L. cv. Samsun und Hordeum vulgare L. cv. Golden Promise erzeugt, die in der Lage waren die Phytase aus Klebsiella sp. ASR1 bzw. aus Bacillus amyloliquefaciens FZB45 zu produzieren. Es wurde für jedes Protein eine Strategie zur Reinigung des aktiven Enzyms aus den verschiedenen Wirtsorganismen entwickelt und seine biochemischen Eigenschaften charakterisiert. Es wurde gezeigt, dass die β-Propeller-Phytase aus Bacillus im Gegensatz zur sauren Phytase aus Klebsiella durch die posttranslationale Modifikation teilweise ihre Eigenschaften ändert. Die Auswirkungen der heterologen Expression der Phytasen auf die Veränderung der Anteile von Phytinsäure und anorganischem Phosphor, in Relation zum gesamten Phosphor, in den Gerstensamen wurden untersucht. Es wurde eine Reduktion des Phytinsäuregehaltes um 19 % und eine Erhöhung des Gehalts anorganischem Phosphor zwischen 27 % und 78 nachgewiesen. Mit Hilfe von spezifischen Signalsequenzen gelang es die Phytaseproteine aus der Wurzel in das umgebende Medium zu sekretieren. Die Sekretion der Bacillus Phytase führte zu einer Steigerung der Biomasseproduktion von Nicotiana tabacum L. unter unsterilen Wachstumsbedingungen mit Phytat als einziger P-Quelle um 34 %.Due to the bad availability of phosphorus in natural habitats the improvement of phosphorus accessibility to organisms became an important topic of research, particularly for agriculture and animal nutrition. In plant seeds phosphorus is bound to D-myo-inositol to form phytic acid that is indigestible for mono gastric animals. Therefore the use of phytases to hydrolyze the phytic acid and to mobilize the anorganic phosphorus came in focus to science. Genetic engineering gave the opportunity to improve the phosphorus availability. Genetic manipulation of plants is a suitable tool to produce phosphorus releasing enzymes, thereby increasing the biomass and decreasing the content of phytic acid in plants. In this work I generated transgenic plants of Nicotiana tabacum and Hordeum vulgare vulgare which successfully express the phytase gene phyK from Klebsiella pneumonia ASR1 and phyC from Bacillus amyloliquefaciens FZB42. Chromatographically purification strategies were developed and biochemical properties were characterized for all phytase proteins. All enzymes were active and PhyC was posttranslational modified. The effect of the recombinant phytase activity to total phosphorus, phytic acid and anorganic phosphorus content of barley seeds were elucidated. The phyK-expression in barley seeds yield a reduction of phytic acid content of about 19 % and an increase of anorganic phosphorus of about 78 %. Transgenic barley seeds with phyC gene expression show an increase of anorganic phosphorus content from 27 % to 48 % but no decrease in phytic acid content. In growth experiments no special phenotypes of plant containing the recombinant protein were visible. The insertion of apoplastic signal sequences in front of the phytase genes resulted in secretion of the proteins into the rhizosphere. The secretion of PhyC led to an improved growth of Nicotiana tabacum under unsterile conditions with sodium phytate as single phosphorus source increasing the biomass up to 34 %.
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Liu, Peng. "Functional studies of type II heterodimeric phytochromes and end-modified type I phyAs in arabidopsis". Diss., Montana State University, 2011. http://etd.lib.montana.edu/etd/2011/liu/LiuP0811.pdf.
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Phytochromes (phys) are a family of dimeric chromoprotein photoreceptors that modulate plant physiological and developmental processes in response to red (R) and far-red (FR) light. In Arabidopsis thaliana, these fall into two functional groups, type I phyA and type II phyB-E. Previous findings have shown that heterodimerization occurs in type II phytochromes and suggest that diverse dimer forms may have specific functions. The first objective of this study was to characterize the activities of individual phytochrome dimer combinations by developing a novel in vivo protein engineering system. Either obligate homodimers or heterodimers of phytochrome N-terminal regions were produced in phyB mutant plants. With this system, a highly active phyB/D heterodimeric form was shown to rescue the phyB mutant phenotype. Dimers of phyB/achromo-phyB, phyB/C, and phyB/E mediated organ-specific growth in de-etiolation by functioning differentially in cotyledons but not in hypocotyls. Light labile phyA is critical in the plant transition from skotomorphogenic to photomorphogenic growth. To investigate possible in vivo phyA heterodimerization with type II phys and the relationship between phy quaternary structure and signaling mechanisms, transgenic plants were generated that express different myc- tagged N- or C-terminal end fusion phyA proteins in a Landsberg erecta (Ler) phyA mutant or a wild-type background. Co-immunoprecipitation showed that phyA only forms homodimers with itself. Compared with fully active one myc epitope (myc1)-tagged phyAs, six myc epitopes attached to the ends of the N- or C- terminus of phyA impaired phyA-mediated far-red high irradiance (FR-HIR) signaling and also attenuated degradation in the light, indicating that alteration of phyA architecture may damage protein-protein interaction both in phyA downstream signaling and in its protein turnover. Overall, these findings have expanded the structurally complex R/FR sensing systems in plants and have implications for how plant growth and development may be fine-tuned through phy heterodimer-mediated tissue-specific growth or phy-modified activity.
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Van, Staden Jason. "Identification and characterisation of a Cryptococcus laurentii Abo 510 Phytase". Thesis, Stellenbosch : Stellenbosch University, 2004. http://hdl.handle.net/10019.1/49982.
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Thesis (MSc)--University of Stellenbosch, 2004.ENGLISH ABSTRACT: Phosphorus is vital for growth of all life forms and is a fundamental component of nucleic acids, ATP and several other biological compounds. Oilseeds and cereal grains, two major constituents of the diet of animals, contain phytic acid, which is the main storage form of phosphorus in plant cells. Monogastric animals, such as poultry and pigs, are not capable of utilising the bound phosphorus in phytic acid since they do not produce phytase, the essential hydrolysing enzyme. Microbial phytase is therefore added to the animal feed to enhance the availability of phosphorus and thus minimise phosphorus pollution and phosphorus supplementation in diets. For a phytase to be effective in the poultry and swine industry, it needs to be able to release phytic acid phosphorus in the digestive tract, it must be thermostable to resist feed processing and must be inexpensive to produce. One approach for developing an efficient phytase for the animal feed industry is by identifying new phytases from microorganisms, plants and animals. In this study, 11 strains of the genus Cryptococcus were screened for 'phytase activity. Initially, a differential agar plate screening method was employed to determine if any Cryptococcus species were able to express phytase, after which production was confirmed in different liquid media. Cryptococcus laurentii Abo 510 was identified as a strain with significant phytase activity. The C. laurentii Abo 510 strain showed clear zones on the differentialmedia agar plates and the production of phytase at high levels was observed when using wet cells grown in liquid media. The C. laurentii Abo 510 strain produced maximal phytase activity at a relatively high temperature (62°C) and in an acidic pH range (pH 5.0). This phytase also showed a broad substrate specificity that may assist in the release of other phosphate compounds captured in feedstuff. Although the phytase did not require any metal ions for its activity, several metal ions caused inhibition of the phytase activity. The enzyme was stable when exposed to 70°C for up to 180 minutes with only 40% loss in activity. Phosphorus addition to the culture media and enzyme assay solution at concentrations exceeding 500 f.!Minhibited the phytase activity completely. Different carbon sources in the culture media also influenced the phytase activity. The enzyme was determined to be a cell wall-associated phytase with little intracellularactivity.
AFRIKAANSE OPSOMMING: Lewende organismes benodig fosfaat vir groei en oorlewing en fosfaat vorm 'n fundamentele komponent van nukleïensure, ATP en verskeie ander biologiese verbindings. Veevoer bestaan meestal uit twee groot bestanddele, naamlik oliesade en graansoorte wat fitiensuur bevat. Fitiensuur is die vernaamste vorm waarin fosfaat in veevoer gestoor word. Enkelmaagdiere soos pluimvee en varke is nie in staat om die fosfaat van die fitiensuur te benut nie, aangesien hierdie diere nie die geskikte hidrolitiese ensiem, fitase, vir die vrystelling van fosfaat besit nie. 'n Mikrobiese fitase-ensiem word derhalwe by veevoer gevoeg om die fosfaatbeskikbaarheid te verhoog. Sodoende word fosfaatbesoedeling en fosfaataanvullings tot die dieet van diere ook verminder. Vir 'n fitase om effektief in die pluimvee en vark-industrie te wees, moet dit fosfaat vanaf fitiensuur in die spysverteringskanaal vrystel, dit moet behandeling by hoë temperature tydens die veevoervervaardiging oorleef en die ensiem moet goedkoop geproduseer kan word. Een van die benaderings om 'n effektiewe fitase vir die dierevoer-industrie te ontwikkel, is om nuwe fitases in mikroërganismes, plante of diere te identifiseer. In hierdie studie is die fitase-aktiwiteit van 11 stamme van die Cryptococcus genus bepaal. Die seleksie vir die produksie van fitase deur die verskillende Cryptococcus stamme was aanvanklik op differensiële agar plate gedoen en in verskillende vloeisto:finedia bevestig. 'n Cryptococcus laurentii Abo SlOstam is geïdentifiseer as 'n goeie fitase produseerder. Die C. laurentii Abo SlOstam het helder sones op die differensiële media agar plate getoon en die produksie van hoë fitase-aktiwiteit is in nat selle waargeneem na opkweking in vloeisto:finedia. Die C. laurentii Abo 510ras produseer maksimum fitase-aktiwieit by 'n redelike hoë temperatuur (62°C) en in 'n suur pH reeks (pH 5.0). Die fitase het ook 'n wye substraatspesifisiteit wat tot die vrystelling van fosfaat vanaf ander komponente in die veevoer mag bydra. Die fitase het geen metaalione vir sy aktiwiteit benodig nie, maar sekere metaalione het die fitase-aktiwiteit onderdruk. Die ensiem was redelik stabiel by 70°C en het na 180 minute blootstelling slegs 'n 40% verlies in aktiwiteit getoon. Die byvoeging van fosfaat in die kultuurmedium en in die ensiem reaksiemengsel teen konsentrasies bo 500 f.lM, het die fitase aktiwiteit heeltemalonderdruk. Verskeie koolstofbronne het ook 'n effek op die optimale fitase-aktiwiteit getoon. Die fitase ensiem is met die selwand geassosieer en het baie min intrasellulêre aktiwiteit getoon.
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Khan, Naheeda. "Effect of phytase on availability of phosphorus to growing pigs". Thesis, University of Nottingham, 1995. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.294729.
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Dorje, Gyurme. "Guhyagarbhatantra and its XIVth century commentary phyogs-bcu mun-sel". Thesis, Online version, 1987. http://ethos.bl.uk/OrderDetails.do?did=1&uin=uk.bl.ethos.283769.
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Li, Jia. "Secretion of active recombinant phytase from stably transformed soybean cells". Diss., This resource online, 1995. http://scholar.lib.vt.edu/theses/available/etd-10042006-143901/.
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Samat, Noraini. "Improvement of phytase efficacy in poultry through dietary fat supplementation". Thesis, University of Leeds, 2015. http://etheses.whiterose.ac.uk/18032/.
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Phosphorus in phytate is largely unavailable to chickens unless they are provided with dietary phytase. Phytase was shown to increase phytate degradation in the crop and proventriculus-gizzard and very little phytate degradation occurred in the duodenum-jejunum or ileum. These previous investigations were conducted on chickens fed corn based diets but not with wheat based diet. Increase in digesta passage or mean retention time (MRT) along the gastrointestinal tract could enhance phytase efficacy as the prolonged reaction time between substrates and phytase may further facilitate phytate dephosphorylation. Dietary fat and fibre supplementation have been shown to influence intestinal MRT in chickens therefore it is expected that inclusion of both dietary fat and fibre could be manipulated to further improve phytase efficacy in broiler chickens. Three experiments and preliminary studies were conducted: 1) to assess the effect of methodology on estimation of phytate hydrolysis and P digestibility in young broilers and 2) to investigate whether or not dietary fat and fibre could further improve phytase efficacy in broilers fed diets containing wheat by delaying digesta MRT. In the preliminary study, in order to facilitate analysis of limited digesta samples, a sample preparation protocol involving H2SO4 digestion for sequential analysis of titanium and other minerals in feed and digesta was established. It was also found that blood protein interfered with the colorimetric analysis of P and myo-inositol, thus deproteinization is required. Continuous feeding prior to sampling was recommended to obtain adequate amount of digesta for digestibility related analyses. In the first study, feeding duration (1 h or 5 h) and 1 h feeding followed by feed withdrawal and refeeding prior to sampling significantly affected the concentration of phytate, inositol phosphates and measured degradation and digestibility of phytate-P in different segments of gastrointestinal tract, which could lead to overestimation or underestimation of degradation and digestibility values. Therefore, it is also recommended to collect digesta samples at least 3 h after the start of photoperiod and avoid sampling 4 h prior to dark period when lighting program is applied. In the second study, 5% fat inclusion had no significant effect on growth performance, while phytase supplementation at 1500 FTU/kg improved feed intake (FI) and body weight gain (BWG). However, interactions between both factors additively increased FI from 804g to 1,221g, BWG from 630 g to 904 g, ileal phytate degradation by 44% and ileal P digestibility by 17%. Adding cellulose as filler in pellet-crumbled diet has diluted the nutrient concentration in 5% fat diet that eventually led to poorer FCR. However, phytase supplementation eliminated the negative effect of nutrient dilution by improving performance of chickens as good as those fed those fed 5% fat diet without cellulose addition. In the third study, a combination of 1500 FTU/kg phytase supplementation with 5% fat increased crop digesta MRT and improved phytate-P degradation compared to with 1% fat, providing evidence for the role of fat in improving phytase efficacy in broilers. This thesis has shown the importance of methodology standardization in estimating phytate hydrolysis and P digestibility and the role of dietary fat and fibre in improving phytase efficacy in broilers.
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Laird, Steven. "The effects of super-dosing phytase in the growing pig". Thesis, University of Leeds, 2016. http://etheses.whiterose.ac.uk/16576/.
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Microbial phytase enzymes are commonly added to monogastric diets at 500 FTU/kg feed (as fed) to improve phytate phosphorus (P) bioavailability and reduce P excretion. The use of super-doses of phytase ( > 1,500 FTU/kg as fed) is currently generating much interest, as recent studies have demonstrated that such high doses can improve the growth performance of monogastrics. However, the underlying mechanism for this response remains unclear. At present, there is a dearth of information available on the effects of super-dosing in the pig. Therefore, this research set out to determine the effects of super-dosing phytase on the growing pig, with a view to shedding light on underlying mechanism, particularly in relation to the possible involvement of myo-inositol (MYO). Through three separate feeding trials, this work found that super-dosing phytase improved the growth performance of weaner pigs, but had no influence on the growth performance of grower or finisher pigs. Ileal digesta were collected and analysed for inositol phosphate content by HPIC. Standard doses of phytase resulted in a small accumulation of InsP4 and InsP3 phytate esters in the ileal digesta of weaner and finisher pigs. This build-up was effectively diminished when supplemented with a phytase super-dose, resulting in more complete phytate degradation. High doses of phytase consistently increased circulating levels of MYO in both the portal and peripheral blood in weaner, grower and finisher pigs. Super-doses of phytase were consistently associated with increases in Ca, P and Mg bioavailability across all stages of production. Moreover, there were also indications of improved trace mineral (Zn, Cu, Fe, Mn) digestibility; however, this effect was less consistent. From this thesis it can be concluded that super-doses of phytase are associated with an increase in phytate hydrolysis and a concomitant increase MYO absorption. However, these effects do not always translate into improved pig performance. Further research is needed to elucidate the factors responsible for the inconsistencies observed.
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Fullat, i. Genis Octavi. "El anthropos es naturaleza physis, historia polis y desmesura ruah". Pontificia Universidad Católica del Perú, 2014. http://repositorio.pucp.edu.pe/index/handle/123456789/116898.
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Röck, Tina [Verfasser]. "Physis als bewegte Existenz. : Eine Ontologie des Konkreten. / Tina Röck". Berlin : Duncker & Humblot, 2016. http://d-nb.info/1238436773/34.
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Pace, Steven Colin. "The effect of high phytase inclusion in laying hen diets". Thesis, University of Leeds, 2014. http://etheses.whiterose.ac.uk/8991/.
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Supplemental phytase is routinely added to monogastric diets to increase the availability of phytate phosphours. Higher phytase inclusion levels (>2,500 FTU kg-1) are attracting interest as extra-phosphoric benefits are seen with increasing phytic acid hydrolysis. Phytic acid hydrolysis has been shown to increase protein, mineral and energy utilisation, reduce endogenous losses and increase the availability of myo-inositol. It is through these improvements that phytase has been shown to benefit broilers and pigs, but not layers. Four experiments were conducted to assess the effect of high phytase inclusion in the laying hen. In the first experiment, phytase was shown to increase the solubility of the diet within the gastrointestinal tract, and in particular the solubility of numerous minerals. Notably, calcium and phosphorus solubility were increased from 4 and 7.4 to 12.3 and 15.9 g soluble 100 g-1 total respectively within the duodenum. Phytase also increased the concentration of calcium, magnesium, zinc and manganese but not phosphorus within the blood plasma of laying hens. In the second experiment, phytase significantly improved the FCR of laying hens from 2.08 to 2.02 and had an additive effect with glucanase in terms of weight gained. The third experiment showed that higher phytase inclusion could increase egg mass in comparison to a positive control, negative control and standard phytase inclusion diet. Chickens fed a diet containing 4,000 FTU kg-1 produced 61.07g/d egg mass whereas birds fed a 250 FTU kg-1 produced just 58.62g/d. In the final experiment, phytase reduced laying hen FCR from 2.11 to 2.03 in the absence of betaine, but with betaine included in the diet phytase had a negative effect increasing FCR from 1.99 to 2.07. Throughout the four experiments phytase was shown to inconsistently benefit shell quality, which may be due to elevated dietary calcium levels in the second and third experiments. Finally, in the third and fourth experiments phytase was shown to increase the concentration of hepatic and circulating myo-inositol respectively. Birds fed diets containing 16,000 FTU kg-1 had hepatic concentration of myo-inositol greater than double those fed a diet with 250 FTU kg-1. This thesis has shown that high phytase inclusion can benefit laying hen performance which may allow dietary nutrient dilutions.
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Comyns, Bruce Henry. "Identification and Distribution of Urophycis (Gill) and Phycis (Artedi) Larvae and Pelagic Juveniles in the Middle Atlantic Bight". W&M ScholarWorks, 1987. https://scholarworks.wm.edu/etd/1539617573.
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Ziaei, Nemat. "Optimisation of diet composition for phytase application strategies in broiler chickens". Thesis, University of Newcastle Upon Tyne, 2009. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.505837.
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Kovačić, Franjo. "Der Begriff der Physis bei Galen vor dem Hintergrund seiner Vorgänger /". Stuttgart : F. Steiner, 2001. http://catalogue.bnf.fr/ark:/12148/cb38803752r.
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Kerovuo, Janne. "A novel phytase from Bacillus : characterization and production of the enzyme". Helsinki : University of Helsinki, 2000. http://ethesis.helsinki.fi/julkaisut/mat/bioti/vk/kerovuo/.
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Le, Xuan Thinh, Xuan Hien Dang y Ngoc Trinh Truong. "Effect of phytase on the water quality of Vietnamese pangasius ponds". Technische Universität Dresden, 2018. https://tud.qucosa.de/id/qucosa%3A33314.
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Striped catfish (Pangasianodon hypophthalmus) is freshwater fish that is raising mainly in the Mekong Delta. The research was implemented at the Model Farm (College of Aquaculture and Fisheries), Can Tho University during 6 months from May to November of 2016 and aimed to evaluate the water quality between two treatments of feed in the pangasius production for proposing a better environmental management method. The treatment 1 (control treatment, CT) has two ponds used feed without adding phytase and treatment 2 (experiment treatment, ET) includes two ponds used feed with adding phytase. The results showed that the temperature, pH, DO, TSS, COD, BOD, N-NO2-, N-NO3-, TKN, TAN were not significantly different (p>0.05) between the CT and ET ponds. At the experiment pond (EP), the factors (TP: 2.28 mg/L; P-PO4 3-: 1.24 mg/L) which has P release to the environment are less than control pond (CP) (TP: 2.62 mg/L; P-PO4 3-: 2.13 mg/L). These results suggested that the addition of phytase in feed could contribute to reducing the nutrient to the water body of pangasius pond.Cá tra (Pangasianodon hypophthalmus) là loài thủy sản nước ngọt được nuôi chủ yếu ở các tỉnh Đồng bằng sông Cửu Long. Nghiên cứu được thực hiện trong 6 tháng từ tháng 05 – 11/2016 tại Trang trại mẫu đặt tại Khoa Thủy sản, Trường Đại học Cần Thơ nhằm đánh giá chất lượng nước giữa 2 nghiệm thức thức ăn trong giai đoạn nuôi cá thịt để đề xuất biện pháp quản lý chất lượng nước ao nuôi tốt hơn. Nghiệm thức 1 (nghiệm thức đối chứng, CT) gồm 2 ao nuôi sử dụng thức ăn không bổ sung enzyme phytase, nghiệm thức 2 (nghiệm thức thí nghiệm, ET) gồm 2 ao nuôi sử dụng thức ăn có bổ sung enzyme phytase. Kết quả thí nghiệm cho thấy giá trị nhiệt độ, pH, DO, TSS, COD, BOD, H2S, N-NO2-, N-NO3-, TKN, TAN ở mức phù hợp cho ao đối chứng (CT). Tại ao thí nghiệm (ET) (TP:2,28 mg/L; P-PO4 3-: 1,24 mg/L) lượng P thải ra ít hơn so với ao CT (TP: 2,62 mg/L; P-PO4 3-: 2,13 mg/L) chứng tỏ việc bổ sung enzyme phytase trong thức ăn đã góp phần làm giảm lượng lân thải ra môi trường nước trong nuôi cá tra.
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Ridley, Timothy J. "A Psychology of Complementarity| Toward a Synochi of Psyche and Physis". Thesis, Pacifica Graduate Institute, 2018. http://pqdtopen.proquest.com/#viewpdf?dispub=10790614.
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This hermeneutic research attempts to address the mind and body problem using complementarity from quantum physics and dual aspect monism from philosophy. Quantum mechanics and dual-aspect monism offer ways to explain complex phenomena that include aspects that are seemingly contradictory. In quantum physics, wave–particle complementarity describes how an atom is both a particle and a wave. In dual-aspect monism, the underlying domain of the universe is neither physical nor mental, but these are both aspects of the ontology. Applying these ideas from quantum mechanics and dual-aspect monism to the existing paradigms within psychology provides different perspectives on the mind-body problem. To begin the process of applying these theories, the physis is imagined to be a particle and the psyche is imagined to be a wave. Complementarity is then used to explore the psyche and the physis. Psyche and physis are also surveyed using dual aspect monism. As the psyche and physis are explored as two different aspects of one ontology, this research attempts to examine how this would manifest within our existence, and what the theories would mean for the splits within the field of psychology. This research found that the frame used to approach psychology (psyche or physis) impacts the results, and trying to approach psychology without using one aspect or the other is difficult to symbolize, and or practice. For depth psychology, this research has shown that retreating entirely to the imaginal or the unconscious may be an unbalanced approach. Keywords: psychology, quantum physics, complementarity, dual-aspect monism
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Mousley, A. "Cross-phyla studies of helminth and arthropod neuropeptides". Thesis, Queen's University Belfast, 2001. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.368480.
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Sebastian, Sylvester. "The effects of supplemental microbial phytase on nutrient utilization in broiler chickens /". Thesis, McGill University, 1996. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=34448.
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The influence of microbial phytase on growth performance, availability of macro and trace minerals, apparent ileal digestibility (AID) and apparent "fecal" digestibility (AFD) of amino acids (AA) and CP were investigated. The optimum level of dietary Ca and P for the maximum efficacy of supplemental phytase in broiler chickens was also studied. Phytase supplementation (600 U/kg) to a low P diet increased $(P 0.05)$ on feed efficiency in broiler chickens at 21 d. The efficacy of phytase, particularly in stimulating growth, was higher in male than female chickens. The relative retention of Ca, P, Cu, Zn and N increased by addition of phytase to a low P diet but phytase had no effect $(P >0.05)$ on the retention of Mg, Mn and Fe. Phytase supplementation increased $(P 0.05)$ on plasma Zn, Cu, and Mg. Phytase increased $(P 0.05)$ on mineral proportions in the tibia ash; however, it increased $(P 0.05)$ on AID of any of the AA in male chickens. Addition of phytase did not have any effect $(P > 0.05)$ on AFD of any of the AA in male chickens but increased $(P 0.05)$ on either AID or AFD of CP and AA at 21-d. In summary, phytase supplementation increased the growth performance, availability of P, Ca, Cu, Zn and N, plasma P, and tibia ash and reduced plasma Ca; it also increased the AID and AFD of most of the AA, particularly in female chickens at 28-d. The efficacy of microbial phytase was high when dietary P and Ca levels were low.
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Chan, Wing-lee. "A study of the expression of beta-propeller phytase in transgenic plants". Click to view the E-thesis via HKUTO, 2004. http://sunzi.lib.hku.hk/hkuto/record/B31049291.
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Sebastian, Sylvester. "The effects of supplemental microbial phytase on nutrient utilization in broiler chickens". Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1997. http://www.collectionscanada.ca/obj/s4/f2/dsk2/tape16/PQDD_0012/NQ30380.pdf.
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Chan, Wing-lee y 陳永利. "A study of the expression of beta-propeller phytase in transgenic plants". Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2004. http://hub.hku.hk/bib/B31049291.
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Walk, Carrie. "Evaluation of the efficacy of high levels of microbial phytase in broilers". Diss., Columbia, Mo. : University of Missouri-Columbia, 2006. http://hdl.handle.net/10355/4630.
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Thesis (M.S.)--University of Missouri-Columbia, 2006.The entire dissertation/thesis text is included in the research.pdf file; the official abstract appears in the short.pdf file (which also appears in the research.pdf); a non-technical general description, or public abstract, appears in the public.pdf file. Title from title screen of research.pdf file (viewed on April 22, 2009) Includes bibliographical references.
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Ragon, Mélanie. "La phytase de Debaryomyces castellii CBS 2923 : surexpression, propriétés catalytiques et structure". Montpellier 2, 2006. http://www.theses.fr/2006MON20130.
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Vidal, Marion. "Sortir des phytos en viticulture : pratiques professionnelles, action collective et normalisation environnementale". Thesis, Toulouse 2, 2015. http://www.theses.fr/2015TOU20058/document.
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Les pesticides utilisés en agriculture posent aujourd’hui des problèmes avérés sur le plan sanitaire et environnemental. Malgré l’implication du monde agricole et l’apport de financements publics significatifs, les politiques publiques déployées depuis 40 ans en matière d’agri-environnement peinent à atteindre leurs objectifs, là où les systèmes de production durables développés en réaction au modèle productiviste s’avèrent probants mais marginaux. La transition vers des systèmes de production plus respectueux de l’environnement demeure ainsi un défi pour les pouvoirs publics et l’agriculture conventionnelle : comment s’organiser collectivement pour « sortir des phytos » ? A partir de l’exemple d’un dispositif expérimental visant la suppression des herbicides en viticulture et initié par des professionnels de la filière, la thèse propose de réinterroger, par le prisme d’une sociologie de l’action, la capacité d’innovation du monde agricole et de réaction des pouvoirs publics en matière d’agri-environnement, en prenant comme clé de lecture l’analyse de l’action collective professionnelle se développant en-deçà des dispositifs institués d’action publique. La thèse montre que, dans de telles conditions, les objets, les mobiles et les collectifs de l’action environnementale ont pour propriétés d’être hétérogènes, équivoques, variables et flexibles, tout au long de leur mise en débat. Qu’il s’agisse des solutions alternatives mises en œuvre ou de leurs capacités de transfert, la pluralité et la variation des situations soulignent une impossibilité de standardisation, susceptible d’appeler des réponses institutionnelles différenciées pour un même problème d’environnementPesticides used in agriculture are a known environmental problematic fact. Despite the involvement of agriculture and the contribution of significant public funding, agri-environmental public policies deployed for over 40 years struggle to achieve their goals, where sustainable production systems remain convincing but marginal. Faced with this context, designing and spreading alternative techniques to chemicals remains a challenge for government and professionals. From case of a professional project aiming at removing herbicides in the South West of France vineyards, this thesis proposes to re-examine, through the prism of a sociology that pay attention to action (less than actors), the innovation capacity of agriculture and the responsiveness of public authorities about agri-environment, focusing on the analysis of professional collective action as a way of regulating environmental public issues, and considering its development out, in parallel, upstream or below established public policies offer. The research shows that in such conditions, objects, mobiles and collectives comprised by environmental action are heterogeneous, ambiguous, variable and flexible, throughout their discussions. Plurality and change situations emphasize as much unpredictability of collective action that impossibility of standardization, that are likely to call (on the contrary) differentiated institutional responses to the same environmental problem
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Wolff, Gabriella Hannah. "Genealogical Correspondence of Learning and Memory Centers across Phyla". Diss., The University of Arizona, 2015. http://hdl.handle.net/10150/556847.
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Across bilaterian phyla, learning and memory allows animals to benefit from central-place foraging, return to ideal food sources, choose mates and avoid dangerous or harmful external stimuli. Although these behaviors are comparable in both vertebrate and invertebrate animals, it is unknown whether or not they are mediated by homologous brain structures. In insects, paired, lobate forebrain structures called mushroom bodies receive input from primary sensory neuropils and are necessary for learning and memory, whereas in crustaceans, this behavior is mediated by paired, compact forebrain structures called hemiellipsoid bodies. Mammalian learning and memory is mediated by the paired, horn-shaped hippocampi, which also receive sensory input and are likewise situated in the forebrain. Did these structures evolve independently along with the ability for animals to learn and remember associations and places? Alternatively, the hypothesis posited in this dissertation is that the last bilaterian ancestor already possessed the ability to learn and adapt to its environment, behavior mediated by paired forebrain structures that evolved divergently into the elaborated forms we observe in extant, crown-group taxa. This hypothesis is investigated and discussed in the following reports: 1) a review of insect brain anatomy and functional connectivity, including a description of mushroom bodies, in the context of arthropod evolution; 2) a comparison of neuroanatomy, circuitry, and protein expression between insect mushroom bodies and Malacostracan crustacean hemiellipsoid bodies, using cockroaches and Caribbean hermit crabs as representatives of their classes; 3) a deeper investigation of the fine structure of neuronal organization in the hemiellipsoid body of the Caribbean hermit crab, focusing on electron microscopical observations and comparisons to the ultrastructure of the fruit fly mushroom body; 4) a survey of four invertebrate Phyla, employing the strategy of comparing neuroanatomy and protein expression to investigate whether higher order forebrain structures in these animals were inherited from a common ancestor; 5) a comparison of neuroanatomy, connectivity, and protein expression in insect mushroom bodies and mammalian hippocampus, including a survey of PKA-Cα in these and corresponding structures across the Chordata. The total evidence suggests that a common Bilaterian ancestor possessed a center that evolved to become mushroom bodies in invertebrates and hippocampus in vertebrates.
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Knobloch, Eberhard. "Nomos und physis : Alexander von Humboldt und die Tradition antiker Denkweisen und Vorstellungen". Universität Potsdam, 2010. http://opus.kobv.de/ubp/volltexte/2011/5175/.
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Alexander von Humboldt’s world view, philosophy of science, and scientific practice were dominated by the notions of measure and harmony. This matter of fact does not leave any doubt about Humboldt’s Pythagoreanism. Already the choice of the title of his Kosmos was led by Pythagorean thought. In 1846 he explicitly wrote to Jacobi that the whole first book is based on the idea that the first Hellenic seeds to all further progresses of mathematical natural knowledge are owed to the Pythagoreans and their emphasis on measure, number, and weight. The paper consists of four parts. The first part deals with Humboldt’s acquaintance with the fragments of the Pythagorean Philolaus. The second part compares the Aristotelean tradition with Philolaus’s informations on Pythagoreanism. The third part relying on the two foregoing parts explains Humboldt’s philosophy of science as a tranformation of Pythagoreanism. The last part discusses three examples taken from Humboldt’s scientific practice in order to illustrate the results deduced up to then.
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Leung, Miu-fun. "Persistence of [beta]-propeller phytase in soil and its implication in phosphorus mobilization". Click to view the E-thesis via HKUTO, 2004. http://sunzi.lib.hku.hk/hkuto/record/B30736511.
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Steiner, Tobias [Verfasser]. "Factors Affecting the Efficacy of Microbial and Plant Phytase in Pigs / Tobias Steiner". Aachen : Shaker, 2006. http://d-nb.info/1170532489/34.
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Wendt, Peter [Verfasser]. "Beiträge zur Standardisierung der Bewertung von Phosphorquellen und Phytase mit Enten / Peter Wendt". Aachen : Shaker, 2004. http://d-nb.info/1181602866/34.
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Leung, Miu-fun y 梁妙芬. "Persistence of {221}-propeller phytase in soil and its implication in phosphorus mobilization". Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2004. http://hub.hku.hk/bib/B30736511.
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Qian, Hao. "Application of microbial phytase and its influencing factors in vivo and in vitro". Diss., This resource online, 1995. http://scholar.lib.vt.edu/theses/available/etd-06062008-155447/.
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Liu, Jiazhong. "Phytate phosphorus hydrolysis by microbial phytase in corn-soybean mean diets for pigs /". free to MU campus, to others for purchase, 1996. http://wwwlib.umi.com/cr/mo/fullcit?p9821350.
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Silva, Marcos AntÃnio Nunes da. "MÃsica cearense atravÃs do canto coral: estudo de caso com o grupo Phylos". Universidade Federal do CearÃ, 2016. http://www.teses.ufc.br/tde_busca/arquivo.php?codArquivo=17796.
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nÃo hÃNa presente pesquisa, trabalhamos com o coral juvenil de uma escola profissionalizante pertencente à rede estadual de ensino de Fortaleza, que utiliza exclusivamente mÃsica cearense em seu repertÃrio atual. Adotando a praxiologia de Pierre Bourdieu, analisamos a formaÃÃo e o desenvolvimento de um coral cÃnico intitulado Phylos, verificando se ocorreu mudanÃa do habitus em seus integrantes durante o perÃodo em que estes participaram do grupo. Para dar suporte a nossa pesquisa, usamos, alÃm de Bourdieu, autores como Theodor Adorno, que aborda a indÃstria cultural como elemento de influÃncia na formaÃÃo do gosto musical dos jovens, e o educador Paulo Freire, que valorizou o conhecimento prÃvio dos alunos em seus estudos e procurou despertar o senso crÃtico dos aprendizes. Ao lado da praxiologia, utilizamos o estudo de caso tambÃm como caminho metodolÃgico. Para a coleta de dados, valemo-nos de entrevistas semiestruturadas, alÃm da observaÃÃo dos ensaios e das aulas de campo que os alunos do grupo citado participaram.
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Spier, Michele Rigon, Adenise Lorenci Woiciechowski, Carlos Ricardo 1953 Soccol y Universidade Federal do Paraná Setor de Tecnologia Programa de Pós-Graduaçao em Processos Biotecnológicos. "Development of a bioprocess for production of a new A. niger FS3 Phytase". reponame:Repositório Institucional da UFPR, 2009. http://hdl.handle.net/1884/18314.
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Silva, Marcos Antônio Nunes da. "Música cearense através do canto coral: estudo de caso com o grupo Phylos". reponame:Repositório Institucional da UFC, 2016. http://www.repositorio.ufc.br/handle/riufc/19718.
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SILVA, Marcos Antônio Nunes da. Música cearense através do canto coral: estudo de caso com o grupo Phylos. 2016. 103f. – Dissertação (Mestrado) – Universidade Federal do Ceará, Programa de Pós-graduação Profissional em Artes, Fortaleza (CE), 2016.Submitted by Gustavo Daher (gdaherufc@hotmail.com) on 2016-09-23T14:18:13Z No. of bitstreams: 1 2016_dis_mansilva.pdf: 2856765 bytes, checksum: 287f181f76a31ec71740e6cffba6d459 (MD5)
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In this present research, we work with the youth choir of a full time vocational school belonging to the State in Fortaleza, which uses exclusively cearense music in its current repertoire. Using a praxiology of Pierre Bourdieu we analyzed the formation and development of a scenic coral entitled Phylos, checking whether there was a change of habitus in its members during the period that they participated in the group.Using to support our research, besides Bourdieu, authors such as Theodor Adorno, that addresses the cultural industry as element that influences the formation of musical taste of young people and the educator Paulo Freire,who in his studies appreciated the students prior knowledge and sought, awaken the critical sense of the learners. Aside of praxiology we also used as a methodological way the case of study.To collect data we used semistructured interviews and the observation of trials and field classes that students of that group participated.
Na presente pesquisa, trabalhamos com o coral juvenil de uma escola profissionalizante pertencente à rede estadual de ensino de Fortaleza, que utiliza exclusivamente música cearense em seu repertório atual. Adotando a praxiologia de Pierre Bourdieu, analisamos a formação e o desenvolvimento de um coral cênico intitulado Phylos, verificando se ocorreu mudança do habitus em seus integrantes durante o período em que estes participaram do grupo. Para dar suporte a nossa pesquisa, usamos, além de Bourdieu, autores como Theodor Adorno, que aborda a indústria cultural como elemento de influência na formação do gosto musical dos jovens, e o educador Paulo Freire, que valorizou o conhecimento prévio dos alunos em seus estudos e procurou despertar o senso crítico dos aprendizes. Ao lado da praxiologia, utilizamos o estudo de caso também como caminho metodológico. Para a coleta de dados, valemo-nos de entrevistas semiestruturadas, além da observação dos ensaios e das aulas de campo que os alunos do grupo citado participaram.
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Wagner, Ashley Leigh. "Phytase Impacts Various Non-Starch Polysaccharidase Activities on Distillers Dried Grains with Solubles". Thesis, Virginia Tech, 2008. http://hdl.handle.net/10919/35343.
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As environmental concerns increase in the U.S., there is a push to go â greenâ . This has fueled the development of ethanol production in the U.S. as a renewable source of energy. In the U.S., corn is used to produce ethanol. Therefore, displacement of corn used in the animal industry to ethanol production has, inevitably caused an increase in the price of corn. Replacing conventional ingredients, such as corn, in monogastric diets with lower cost by products can be attractive economically. The objective of this study was to determine if exogenous enzymes that free reducing sugars from fiber will increase the dispensability of bound nutrients in the economically favorable by-product of ethanol production, distillers dried grains with solubles (DDGS). Effects of non-starch polysaccharidase (NSPase) inclusion rates and combinations with phytase on the ability to release reducing sugars were investigated. Distillers dried grains with solubles was tested in vitro in combination with hemicellulase (0, 0.1, 1, and 10 U/g of DDGS), cellulase (0, 1.5, 15, and 150 U/g of DDGS), xylanase (0, 2.5, 25, and 250 U/g of DDGS), ï ¢ glucanase (0, 0.006, 0.06, and 0.6 U/g of DDGS), and phytase (0, 250, 500, and 1,000 FTU/kg of DDGS). Results indicated an inhibitory effect of phytase on the ability of NSPases to release reducing sugars from DDGS (P = 0.0004). A second study was conducted to determine individual enzyme kinetics of NSPase on pure substrates when combined with increasing concentrations of phytase. Cellulase (P = 0.03), xylanase (P = 0.09), and ï ¢-glucanase (P = 0.06) combined with increasing concentrations of phytase showed a reduction in the velocity of reducing sugars release from pure substrate. However, we speculate that due to the inability to reach Vmax, the Lineweaver-Burk results were inconclusive. The 1/Km was calculated for all enzymes, and the presence of phytase increased 1/Km for xylanase (P = 0.006). However, -1/Km did not change for cellulase (P = 0.62), ï ¢-glucanase (P = 0.20), and hemicellulase (P = 0.13). In addition, the 1/Vmax was calculated for all enzymes, and the presence of phytase decreased 1/Vmax for cellulase (P = 0.03) and ï ¢ glucanase (P = 0.01). However, the presence of phytase resulted in no change for the 1/Vmax for xylanase (P = 0.81) and hemicellulase (P = 0.14). The slopes of the regression lines for the Lineweaver-Burk plots showed no effect of the presence of phytase for cellulase (P = 0.40) and hemicellulase (P = 0.27). However, the presence of phytase decreased the linear slope for xylanase (P = 0.006), and increased the linear slope for ï ¢ glucanase (P = 0.006). In summary, phytase appears to act as an inhibitor of NSPase activity in the in vitro digestion on DDGS; however, inconsistent results from the inhibition curves preclude us from determining the type of inhibition.Master of Science
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Huang, Zirou. "Measurement of Phytase Activity in a Clymer Forest Soil Using the TInsP5 Probe". Thesis, Virginia Tech, 2009. http://hdl.handle.net/10919/43877.
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Measurement of soil phytase activity (PA) and delineation of the impact of this important phosphomonoesterase on the P-cycling process in soil and sediments suffer from the lack of a reliable assay. A method for measuring PA in soil that promises to be accurate and reliable has been recently published. The method involves the use of a novel chromophoric analog of phytic acid, referred to as T(tethered)InsP5 (5-O-[6-(benzoylamino)hexyl]-D-myo-inositol-1,2,3,4,6-pentakisphosphate). This study was conducted to measure PA in a Clymer forest soil, which contained over twice the amount of soil organic C as previously tested soils, using the TInsP5 PA assay. This investigation specifically addresses: (1) the development of a soil dilution technique for determining maximal PA, (2) identification of previously unsubstantiated soil-produced dephosphorylated intermediate probe species, (3) the impact of increasing assay buffer pH on soil PA and (4) testing stability of the probeâ s amide bond in a highly (bio)active forest soil. PA assays were conducted by measuring dephosphorylation of TInsP5 in citrate-acetate buffered (pH 4.2) active and autoclaved (Control) soil suspensions. Phosphorylated probe intermediates (i.e., TInsP4, TInsP3, TInsP2 and TInsP1) and T-myo-inositol were extracted from samples of soil suspension following incubation. Probe species were quantified using reversed phase high-performance liquid chromatography (RPHPLC) with UV detection. PA was calculated based on a mass balance approach. A soil dilution technique was developed to address the challenge of determining maximal PA in soils containing higher organic matter content. In the initial report on use of the TInsP5 method for measuring PA in soil, two â soil-generatedâ UV-adsorbing compounds (designated Y and Z) were observed, but never confirmed as probe species. The experimental evidence presented in this report supports inclusion of compound Y as a phosphorylated probe intermediate species (i.e. TInsPy), based primarily on its UV adsorption spectra (diode-array detection analysis). Compound Z could not be substantiated as a probe species based on the evidence presented in this study. PA of Claymer forest soil decreased with an increase in assay buffer pH. Further, the probeâ s amide bond linkage was stable in a forest soil exhibiting high PA.Master of Science
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Kumar, Kris. "P-type ATPases in the proteobacterial, bacteroidetes and fusobacterial phyla". Diss., [La Jolla] : University of California, San Diego, 2009. http://wwwlib.umi.com/cr/ucsd/fullcit?p1465079.
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Thesis (M.S.)--University of California, San Diego, 2009.Title from first page of PDF file (viewed June 22, 2009). Available via ProQuest Digital Dissertations. Includes bibliographical references (p. 93-94).
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Kelley, John Forad. "Expanding Metabolic Diversity of Two Archaeal Phyla: Nanoarchaeota and Korarchaeota". PDXScholar, 2017. https://pdxscholar.library.pdx.edu/open_access_etds/3835.
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Culture independent studies have revealed a greater diversity of Archaea than the two kingdoms initially defined by Carl Woese. Culturing species from the newly discovered archaeal lineages, as with the majority of microbes, has been difficult. To overcome the culturing dilemma, metagenomics is being used to reconstruct environmental genomes. Two metagenomic studies are presented in this thesis, with the aim of recovering newly proposed archaeal genomes from the Nanoarchaeota and Korarchaeota.
In the first study, a sediment sample, NZ13, was collected from a terrestrial New Zealand hot spring. Along with the sediment sample, two complex enrichments were sequenced on an Illumina MiSeq platform. Assembly and differential binning recovered two nearly complete genomes of a nanoarchaeote and a korarchaeote. The NZ13 nanoarchaeote is similar to other terrestrial nanoarchaeotes, which lack an ATP synthase and encode genes for glycolysis/gluconeogenesis and archaella. One notable difference is the NZ13 nanoarchaeote contains CRISPR genes, which are absent in other terrestrial nanoarchaeotes, although present in a marine nanoarchaeote, Nanoarchaeum equitans. The NZ13 korarchaeote mirrors Candidatus Korarchaeum cryptofilum, lacking genes for de novo synthesis of purines and several cofactors, while containing an abundance of peptide transporters and amino acid fermentation pathways.
The second study focused on sulfide samples collected from deep-sea hydrothermal vent fields in southwestern Pacific Ocean along the Eastern Lau Spreading Center. Ten sulfide samples were sequenced on an Illumina HiSeq platform. Small subunit ribosomal RNA genes were extracted from the metagenome reads and aligned against the SILVA Ref NR 99 123 database. The preliminary results identified which samples could be prioritized for genome reconstruction of uncultured bacterial and archaeal lineages. Three uncultured bacteria, candidate division SR1, Gracilibacteria (GN02), and Parcubacteria (OD1) were identified in several samples. Many uncultured deep-sea hydrothermal archaeal lineages were identified in all samples. In particular, korarchaeotal sequences were in high relative archaeal abundances in two samples, ABE 1 and Vai Lili-2, while few nanoarchaeotal reads were classified.
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Kasano, Soto Gladys Thais, Passano Ariana Isabel Luna, Guerra Diggi Becky Romero, Caro Rodrigo Alfonso Tuesta y Torres Milay Aileen Wong. "Proyecto Phyra. Línea para el cuidado del hogar 100% natural". Bachelor's thesis, Universidad Peruana de Ciencias Aplicadas (UPC), 2019. http://hdl.handle.net/10757/626532.
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El trabajo de investigación se centra en el proyecto Phyra, el cual consiste en la venta online de productos 100% naturales para el cuidado del hogar, los cuales son: repelente, resinas aromatizadoras y antihumedad. Estos productos están dirigidos a Hombres y mujeres del NSE A, B, C1, entre las edades de 25 a 55 años y que residen en Lima Metropolitana; asimismo, que tengan un interés en el cuidado de su salud y sean ecorresponsables.
Los experimentos realizados permitieron que se valide que el público objetivo tenía un problema con los productos actuales y estaban dispuestos a probar nuevas cosas, que finalmente le brinden una solución. Ante esto, se iniciaron las primeras ventas en la página de Facebook y se logró comprobar que Phyra era aceptado por el público. Con el resultado obtenido se buscó renovar el repelente en cuanto a su empaque y desarrollar dos nuevos productos. Todo esto fue también validado a través de las ventas.
El contenido del trabajo se divide principalmente en la descripción del modelo de negocio, luego en la validación del mismo y finalmente, el desarrollo del plan de negocio, que incluye operaciones, RRHH, marketing, RSE y finanzas. Con todo esto se puede justificar la viabilidad del negocio.
Finalmente se pudo concluir que el proyecto es rentable gracias al concierge realizado y al análisis de los estados financieros. Donde se obtuvo que la inversión inicial es de S/. 7,040.30 y se espera recuperarla en el primer año.The research focuses on Phyra project, which consists of the online sale of 100% natural products for home care, which are: repellent, room odorizer resins and anti-humidity. These products are aimed at men and women of NSE A, B, C1, between the ages of 25 to 55 years and who reside in Metropolitan Lima; likewise, that they have an interest in health care and eco-responsible. The experiments carried out allowed the validation that the target audience had a problem with the current products and were willing to try new things, that finally provide a solution. Before this, the first sales on the Facebook page began and it was found that Phyra was accepted by the public. With the obtained result it was sought to renew the repellent in terms of its packaging and develop two new products. All this was also validated through sales. The content of the work is divided mainly in the description of the business model, then in the validation of the same and finally, the development of the business plan, which includes operations, HR, marketing, CSR and finance. With all this you can justify the viability of the business. Finally, it was concluded that the project is profitable thanks to the concierge carried out and the analysis of the financial statements. Where it was obtained that the initial investment is S /. 7,040.30 and it is expected to recover in the first year.
Trabajo de investigación