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Artículos de revistas sobre el tema "Real-time cell analysis"

Autor: Grafiati

Publicado: 9 de noviembre de 2024

Última modificación: 31 de julio de 2025

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1

Vogt, Nina. "Real-time behavioral analysis." Nature Methods 18, no. 2 (2021): 123. http://dx.doi.org/10.1038/s41592-021-01072-z.

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2

Jaiswal, Devina, Armin Tahmasbi Rad, Mu-Ping Nieh, Kevin P. Claffey, and Kazunori Hoshino. "Micromagnetic Cancer Cell Immobilization and Release for Real-Time Single Cell Analysis." Journal of Magnetism and Magnetic Materials 427 (April 2017): 7–13. http://dx.doi.org/10.1016/j.jmmm.2016.11.002.

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3

Pranada, Albert L., Silke Metz, Andreas Herrmann, Peter C. Heinrich, and Gerhard Müller-Newen. "Real Time Analysis of STAT3 Nucleocytoplasmic Shuttling." Journal of Biological Chemistry 279, no. 15 (2003): 15114–23. http://dx.doi.org/10.1074/jbc.m312530200.

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4

Cerignoli, Fabio, Yama A. Abassi, Brandon J. Lamarche, et al. "In vitro immunotherapy potency assays using real-time cell analysis." PLOS ONE 13, no. 3 (2018): e0193498. http://dx.doi.org/10.1371/journal.pone.0193498.

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5

Rappoport, J. Z. "Real-time analysis of clathrin-mediated endocytosis during cell migration." Journal of Cell Science 116, no. 5 (2003): 847–55. http://dx.doi.org/10.1242/jcs.00289.

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6

Ersöz, M., S. Malkoç, EB Küçük, BS Bozkurt, and SS Hakki. "Biocompatibility evaluation of orthodontic composite by real-time cell analysis." Human & Experimental Toxicology 35, no. 8 (2016): 833–38. http://dx.doi.org/10.1177/0960327115607944.

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Introduction: The aim of this study was to evaluate the cytotoxic effects of three different light-cured orthodontic composites. Material and methods: Light Bond (Reliance orthodontic products), Grengloo (Ormco corporation), and Kurasper F (Kuraray Europe GmbH) were selected for the experiment. Specimens were prepared according to the manufacturers’ instructions, measuring 5 mm in diameter and 2 mm in thickness. Fibroblast cells were obtained from healthy gingival connective tissues. The composite cylinders were incubated in Dulbecco’s modified Eagle’s culture medium for 72 h according to ISO

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7

Simons, Peter C., Sean M. Biggs, Anna Waller, et al. "Real-time Analysis of Ternary Complex on Particles." Journal of Biological Chemistry 279, no. 14 (2004): 13514–21. http://dx.doi.org/10.1074/jbc.m310306200.

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8

Mazhar, Muhammad Waqar. "Molecular Analysis of Covid-19 Patient Real Time PCR and their Medicational Clinical Trials." Virology & Immunology Journal 5, no. 3 (2021): 1–4. http://dx.doi.org/10.23880/vij-16000284.

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The corona name derived from their crown like spike proteins attach with cell receptors. It belongs to corona viradae family and nideo virales order, envelop virus, size range 65-125nm and positive single standard RNA between 26.4 to 31.7 kb and contain7096 amino acid. There are four subtypes that have been detected these are alpha, beta, gamma and delta. The 267 covid -19 blood and nasopharyngeal samples were collected from Multan region. RNA extraction from nasopharyngeal samples and run the PCR. The blood samples use for clinical tests, Lactate dehydrogenase, serum ferritin level, D-Dimer,

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9

Aoyama, Tadayoshi, Amalka De Zoysa, Qingyi Gu, Takeshi Takaki, and Idaku Ishii. "Vision-Based Real-Time Microflow-Rate Control System for Cell Analysis." Journal of Robotics and Mechatronics 28, no. 6 (2016): 854–61. http://dx.doi.org/10.20965/jrm.2016.p0854.

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[abstFig src='/00280006/09.jpg' width='300' text='Snapshots of particle sorting experiment using our system' ] On-chip cell analysis is an important issue for microtechnology research, and microfluidic devices are frequently used in on-chip cell analysis systems. One approach to controlling the fluid flow in microfluidic devices for cell analysis is to use a suitable pumps. However, it is difficult to control the actual flow-rate in a microfluidic device because of the difficulty in placing flow-rate sensors in the device. In this study, we developed a real-time flow-rate control system that u

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10

Akere, Millicent T., Kelsee K. Zajac, James D. Bretz, Anvitha R. Madhavaram, Austin C. Horton, and Isaac T. Schiefer. "Real-Time Analysis of Neuronal Cell Cultures for CNS Drug Discovery." Brain Sciences 14, no. 8 (2024): 770. http://dx.doi.org/10.3390/brainsci14080770.

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The ability to screen for agents that can promote the development and/or maintenance of neuronal networks creates opportunities for the discovery of novel agents for the treatment of central nervous system (CNS) disorders. Over the past 10 years, advances in robotics, artificial intelligence, and machine learning have paved the way for the improved implementation of live-cell imaging systems for drug discovery. These instruments have revolutionized our ability to quickly and accurately acquire large standardized datasets when studying complex cellular phenomena in real-time. This is particular

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11

Barbee, K. A., S. Hong, E. Ergezen, S. Kwoun, and R. Lec. "Real time analysis of cell-surface adhesive interactions using TSM sensor." Journal of Biomechanics 39 (January 2006): S242. http://dx.doi.org/10.1016/s0021-9290(06)83911-4.

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12

Slanina, H., A. König, H. Claus, M. Frosch, and A. Schubert-Unkmeir. "Real-time impedance analysis of host cell response to meningococcal infection." Journal of Microbiological Methods 84, no. 1 (2011): 101–8. http://dx.doi.org/10.1016/j.mimet.2010.11.004.

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13

Rehm, Markus, Heiko Düßmann, and Jochen H. M. Prehn. "Real-time single cell analysis of Smac/DIABLO release during apoptosis." Journal of Cell Biology 162, no. 6 (2003): 1031–43. http://dx.doi.org/10.1083/jcb.200303123.

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We examined the temporal and causal relationship between Smac/DIABLO release, cytochrome c (cyt-c) release, and caspase activation at the single cell level during apoptosis. Cells treated with the broad-spectrum caspase inhibitor z-VAD-fmk, caspase-3 (Casp-3)–deficient MCF-7 cells, as well as Bax-deficient DU-145 cells released Smac/DIABLO and cyt-c in response to proapoptotic agents. Real-time confocal imaging of MCF-7 cells stably expressing Smac/DIABLO-yellow fluorescent protein (YFP) revealed that the average duration of Smac/DIABLO-YFP release was greater than that of cyt-c-green fluoresc

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14

Ramis, G., L. Martínez-Alarcón, J. J. Quereda, et al. "Optimization of cytotoxicity assay by real-time, impedance-based cell analysis." Biomedical Microdevices 15, no. 6 (2013): 985–95. http://dx.doi.org/10.1007/s10544-013-9790-8.

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15

Santonastaso, Alice, and Claudia Scotti. "Real Time Cell Analysis of Model Target Cell Lines Exposed to Purified Lipoprotein (a)." British Journal of Medicine and Medical Research 16, no. 4 (2016): 1–12. http://dx.doi.org/10.9734/bjmmr/2016/26869.

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16

Ozdemir, Aysun, and Mustafa Ark. "xCELLigence Real Time Cell Analysis System: A New Method for Cell Proliferation and Cytotoxicity." Niche Journal 2, no. 2 (2014): 15–17. http://dx.doi.org/10.5152/niche.2014.153.

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17

Ye, Julian, Yun Luo, Weijia Fang, et al. "Real-Time Cell Analysis for Monitoring Cholera Toxin-Induced Human Intestinal Epithelial Cell Response." Current Microbiology 70, no. 4 (2014): 536–43. http://dx.doi.org/10.1007/s00284-014-0752-z.

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18

Di Costanzo, Ezio, Vincenzo Ingangi, Claudia Angelini, Maria Francesca Carfora, Maria Vincenza Carriero, and Roberto Natalini. "A Macroscopic Mathematical Model for Cell Migration Assays Using a Real-Time Cell Analysis." PLOS ONE 11, no. 9 (2016): e0162553. http://dx.doi.org/10.1371/journal.pone.0162553.

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19

Lecoeur, Hervé, Alain Langonné, Ludwig Baux, et al. "Real-time flow cytometry analysis of permeability transition in isolated mitochondria." Experimental Cell Research 294, no. 1 (2004): 106–17. http://dx.doi.org/10.1016/j.yexcr.2003.10.030.

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20

Carmichael, Stephen W. "Developmental Dynamics in Real Time." Microscopy Today 17, no. 3 (2009): 3–5. http://dx.doi.org/10.1017/s1551929500050021.

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Embryologic development is a dynamic process that has been previously studied by examining static (usually chemically-fixed) specimens at different time periods and then extrapolating results by assembling a series of static images. Recently, Amy McMahon, Willy Supatto, Scott Fraser, and Angelike Stathopoulos have developed new methods to look at developmental migration patterns in real time. They used an optimized imaging approach and quantitative methods to analyze a two hour period during which gastrulation occurred in the embryos of fruitflies (Drosophila). Specifically, they characterized

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21

Yan, Guojun, Qian Du, Xuchao Wei, et al. "Application of Real-Time Cell Electronic Analysis System in Modern Pharmaceutical Evaluation and Analysis." Molecules 23, no. 12 (2018): 3280. http://dx.doi.org/10.3390/molecules23123280.

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Objective: We summarized the progress of the xCELLigence real-time cell analysis (RTCA) technology application in recent years for the sake of enriching and developing the application of RTCA in the field of Chinese medicine. Background: The RTCA system is an established electronic cellular biosensor. This system uses micro-electronic biosensor technology that is confirmed for real-time, label-free, dynamic and non-offensive monitoring of cell viability, migration, growth, spreading, and proliferation. Methods: We summarized the relevant experiments and literature of RTCA technology from the p

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22

Shah, F. J., C. Caviglia, K. Zór, et al. "Impedance-based real-time monitoring of neural stem cell differentiation." Journal of Electrical Bioimpedance 12, no. 1 (2021): 34–49. http://dx.doi.org/10.2478/joeb-2021-0006.

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Abstract We present here the first impedance-based characterization of the differentiation process of two human mesencephalic fetal neural stem lines. The two dopaminergic neural stem cell lines used in this study, Lund human mesencephalic (LUHMES) and human ventral mesencephalic (hVM1 Bcl-XL), have been developed for the study of Parkinsonian pathogenesis and its treatment using cell replacement therapy. We show that if only relying on impedance magnitude analysis, which is by far the most usual approach in, e.g., cytotoxicity evaluation and drug screening applications, one may not be able to

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23

Gomes, Eliza, Felipe Costa, Carlos De Rolt, Patricia Plentz, and Mario Dantas. "A Survey from Real-Time to Near Real-Time Applications in Fog Computing Environments." Telecom 2, no. 4 (2021): 489–517. http://dx.doi.org/10.3390/telecom2040028.

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In this article, we present a comprehensive survey on time-sensitive applications implemented in fog computing environments. The goal is to research what applications are being implemented in fog computing architectures and how the temporal requirements of these applications are being addressed. We also carried out a comprehensive analysis of the articles surveyed and separate them into categories, according to a pattern found in them. Our research is important for the area of real-time systems since the concept of systems that respond in real time has presented various understandings and conc

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24

Dow, J. A., J. M. Lackie, and K. V. Crocket. "A simple microcomputer-based system for real-time analysis of cell behaviour." Journal of Cell Science 87, no. 1 (1987): 171–82. http://dx.doi.org/10.1242/jcs.87.1.171.

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An image analysis package based on a BBC microcomputer has been developed, which can simultaneously track many moving cells in vitro. Cells (rabbit neutrophil leucocytes, BHK C13 fibroblasts, or PC12 phaeochromocytoma cells) are viewed under phase optics with a monochrome TV camera, and the signal digitized. Successive frames are acquired by the computer as a 640 X 256 pixel array. Under controlled lighting conditions, cells can readily be isolated from the background by binary filtering. In real-time tracking, the positions of a given cell in successive frames are obtained by searching the ar

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25

Toy, Ebubekir, BuketS Bozkurt, SemaS Hakki, Erdem Hatunoglu, and Firat Ozturk. "Real-time cell analysis of cytotoxicity of orthodontic cements on gingival fibroblasts." Journal of Orthodontic Research 2, no. 1 (2014): 32. http://dx.doi.org/10.4103/2321-3825.125922.

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26

Ma, Rui, Zhongliang Li, Elena Breaz, Briois Pascal, and Fei Gao. "Numerical Stiffness Analysis for Solid Oxide Fuel Cell Real-Time Simulation Applications." IEEE Transactions on Energy Conversion 33, no. 4 (2018): 1917–28. http://dx.doi.org/10.1109/tec.2018.2849930.

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27

DEMIREL, Gülbike, Fatma Funda KAYA DEMIRSOY, and Özgür IRMAK. "Cytotoxicity evaluation of eluates from universal adhesives by real-time cell analysis." Dental Materials Journal 39, no. 5 (2020): 815–24. http://dx.doi.org/10.4012/dmj.2019-221.

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28

LeFurgey, A., S. D. Davilla, D. A. Kopf, J. R. Sommer, and P. Ingram. "Real-time quantitative elemental analysis and mapping: microchemical imaging in cell physiology." Journal of Microscopy 165, no. 2 (1992): 191–223. http://dx.doi.org/10.1111/j.1365-2818.1992.tb01481.x.

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29

Lodygin, Dmitri, and Alexander Flügel. "Intravital real-time analysis of T-cell activation in health and disease." Cell Calcium 64 (June 2017): 118–29. http://dx.doi.org/10.1016/j.ceca.2016.12.007.

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30

Fregin, Bob, Fabian Czerwinski, Doreen Biedenweg, et al. "Dynamic Real-Time Deformability Cytometry - Time-Resolved Mechanical Single Cell Analysis at 100 Cells/s." Biophysical Journal 118, no. 3 (2020): 605a. http://dx.doi.org/10.1016/j.bpj.2019.11.3267.

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31

Chigaev, Alexandre, Ann Marie Blenc, Julie V. Braaten та ін. "Real Time Analysis of the Affinity Regulation of α4-Integrin". Journal of Biological Chemistry 276, № 52 (2001): 48670–78. http://dx.doi.org/10.1074/jbc.m103194200.

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32

Eriksson, Ida, Linda Vainikka, Hans Lennart Persson, and Karin Öllinger. "Real-Time Monitoring of Lysosomal Membrane Permeabilization Using Acridine Orange." Methods and Protocols 6, no. 4 (2023): 72. http://dx.doi.org/10.3390/mps6040072.

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Loss of lysosomal membrane integrity results in leakage of lysosomal hydrolases to the cytosol which might harm cell function and induce cell death. Destabilization of lysosomes often precede apoptotic or necrotic cell death and occur during both physiological and pathological conditions. The weak base acridine orange readily enters cells and accumulates in the acidic environment of lysosomes. Vital staining with acridine orange is a well-proven technique to observe lysosomal destabilization using fluorescence microscopy and flow cytometry. These analyses are, however, time consuming and only

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33

Banerjee, Suchitra, K. P. Madhusudanan, Suman P. S. Khanuja, and Sunil K. Chattopadhyay. "Analysis of cell cultures ofTaxus wallichiana using direct analysis in real-time mass spectrometric technique." Biomedical Chromatography 22, no. 3 (2008): 250–53. http://dx.doi.org/10.1002/bmc.919.

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34

Roberts, Adam, and Lior Pachter. "Streaming fragment assignment for real-time analysis of sequencing experiments." Nature Methods 10, no. 1 (2013): 71–73. http://dx.doi.org/10.1038/nmeth.2251.

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35

Ferracci, Géraldine, Michael Seagar, Courageot Joël, Raymond Miquelis, and Christian Lévêque. "Real time analysis of intact organelles using surface plasmon resonance." Analytical Biochemistry 334, no. 2 (2004): 367–75. http://dx.doi.org/10.1016/j.ab.2004.08.002.

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36

Schneider, Joachim, Vera Classen, Monika Philipp, and Simone Helmig. "Rapid analysis of XRCC1 polymorphisms using real-time polymerase chain reaction." Molecular and Cellular Probes 20, no. 3-4 (2006): 259–62. http://dx.doi.org/10.1016/j.mcp.2006.01.004.

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37

Rancurel, Corinne, Trang van Tran, Céline Elie, and Frédérique Hilliou. "SATQPCR: Website for statistical analysis of real-time quantitative PCR data." Molecular and Cellular Probes 46 (August 2019): 101418. http://dx.doi.org/10.1016/j.mcp.2019.07.001.

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38

Liu, Yan, Yunhai Zhang, Qiuling Jiang, et al. "Identification of Valid Housekeeping Genes for Real-Time Quantitative PCR Analysis of Collapsed Lung Tissues of Neonatal Somatic Cell Nuclear Transfer–Derived Cattle." Cellular Reprogramming 17, no. 5 (2015): 360–67. http://dx.doi.org/10.1089/cell.2015.0024.

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39

Shlyapnikov, Yuri M., and Elena A. Shlyapnikova. "Ultrasensitive Bead-Based Immunoassay for Real-Time Continuous Sample Flow Analysis." Biosensors 15, no. 5 (2025): 316. https://doi.org/10.3390/bios15050316.

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The performance of heterophase immunoassays is often limited by the kinetics of analyte binding. This problem is partially solved by bead-based assays, which are characterized by rapid diffusion in the particle suspension. However, at low analyte concentrations, the binding rate is still low. Here, we demonstrate a further improvement of analyte binding kinetics in bead-based immunoassays by simultaneously concentrating both an analyte and magnetic beads in a compact spatial region where binding occurs. The analyte is electrophoretically concentrated in a flow cell where beads are magnetically

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40

Bevan, Nicola, Gillan Lovell, Belinda O’Clair, et al. "Real-time visualization and quantification of neutrophil activation and function using live-cell analysis." Journal of Immunology 202, no. 1_Supplement (2019): 130.30. http://dx.doi.org/10.4049/jimmunol.202.supp.130.30.

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Abstract Polymorphonuclear neutrophils (PMNs) have multiple functions during the resolution of inflammation. At the inflammation site, increases in chemokines induce infiltration of PMNs via CXCR receptor activation, resulting in cell clearance via phagocytosis and NETosis. Here we describe characterization of the activation and function of PMNs using IncuCyte® live-cell analysis. Changes in cell shape and CD marker expression are known indicators of PMN activation. Freshly isolated PMNs were seeded in 96-well plates, in the presence of FabFlour-488 labeled CD11b antibody for live-cell immunoc

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41

Türker Şener, Leyla, Gürcan Albeni̇z, Bi̇rcan Di̇nç, and Işil Albeni̇z. "iCELLigence real-time cell analysis system for examining the cytotoxicity of drugs to cancer cell lines." Experimental and Therapeutic Medicine 14, no. 3 (2017): 1866–70. http://dx.doi.org/10.3892/etm.2017.4781.

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42

Li, Xiaokang, Maria Soler, Crispin Szydzik, et al. "Single Cell Analysis: Label-Free Optofluidic Nanobiosensor Enables Real-Time Analysis of Single-Cell Cytokine Secretion (Small 26/2018)." Small 14, no. 26 (2018): 1870119. http://dx.doi.org/10.1002/smll.201870119.

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43

Warnes, G., and S. Martins. "Real-time flow cytometry for the kinetic analysis of oncosis." Cytometry Part A 79A, no. 3 (2011): 181–91. http://dx.doi.org/10.1002/cyto.a.21022.

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44

Toy, Ebubekir, Siddik Malkoc, Bayram Corekci, Buket S. Bozkurt, and Sema S. Hakki. "Real-time cell analysis of the cytotoxicity of orthodontic brackets on gingival fibroblasts." Journal of Applied Biomaterials & Functional Materials 12, no. 3 (2014): 248–55. http://dx.doi.org/10.5301/jabfm.5000165.

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45

Biwer, Christopher M., Andres Quan, Larissa Q. Huston, Blake T. Sturtevant, and Christine M. Sweeney. "Cinema:Snap: Real-time tools for analysis of dynamic diamond anvil cell experiment data." Review of Scientific Instruments 92, no. 10 (2021): 103901. http://dx.doi.org/10.1063/5.0057878.

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46

Stockinger, Walter, Adam B. Castoreno, Yan Wang, Joanne C. Pagnon, and Axel Nohturfft. "Real-time analysis of endosomal lipid transport by live cell scintillation proximity assay." Journal of Lipid Research 45, no. 11 (2004): 2151–58. http://dx.doi.org/10.1194/jlr.d400011-jlr200.

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47

Sanchez-Freire, Veronica, Antje D. Ebert, Tomer Kalisky, Stephen R. Quake, and Joseph C. Wu. "Microfluidic single-cell real-time PCR for comparative analysis of gene expression patterns." Nature Protocols 7, no. 5 (2012): 829–38. http://dx.doi.org/10.1038/nprot.2012.021.

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48

Wettstein, P., M. Strausbauch, T. Therneau, and N. Borson. "The application of real-time PCR to the analysis of T cell repertoires." Nucleic Acids Research 36, no. 21 (2008): e140-e140. http://dx.doi.org/10.1093/nar/gkn634.

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49

Prasad, Brinda, Shan Du, Wael Badawy, and Karan V. I. S. Kaler. "A real-time multiple-cell tracking platform for dielectrophoresis (DEP)-based cellular analysis." Measurement Science and Technology 16, no. 4 (2005): 909–24. http://dx.doi.org/10.1088/0957-0233/16/4/003.

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50

Hewett, P. J., M. L. Texler, D. Anderson, Grant King, and B. E. Chatterton. "In vivo real-time analysis of intraperitoneal radiolabeled tumor cell movement during laparoscopy." Diseases of the Colon & Rectum 42, no. 7 (1999): 868–75. http://dx.doi.org/10.1007/bf02237091.

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