Bibliografías temáticas / Scratch Wound Healing Assay

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Literatura académica sobre el tema "Scratch Wound Healing Assay"

Autor: Grafiati

Publicado: 4 de junio de 2021

Última modificación: 16 de febrero de 2022

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Artículos de revistas sobre el tema "Scratch Wound Healing Assay"

Alishahedani, Mohammadali E., Manoj Yadav, Katelyn J. McCann, Portia Gough, Carlos R. Castillo, Jobel Matriz y Ian A. Myles. "Therapeutic candidates for keloid scars identified by qualitative review of scratch assay research for wound healing". PLOS ONE 16, n.º 6 (18 de junio de 2021): e0253669. http://dx.doi.org/10.1371/journal.pone.0253669.

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The scratch assay is an in vitro technique used to analyze cell migration, proliferation, and cell-to-cell interaction. In the assay, cells are grown to confluence and then ‘scratched’ with a sterile instrument. For the cells in the leading edge, the resulting polarity induces migration and proliferation in attempt to ‘heal’ the modeled wound. Keloid scars are known to have an accelerated wound closure phenotype in the scratch assay, representing an overactivation of wound healing. We performed a qualitative review of the recent literature searching for inhibitors of scratch assay activity that were already available in topical formulations under the hypothesis that such compounds may offer therapeutic potential in keloid treatment. Although several shortcomings in the scratch assay literature were identified, caffeine and allicin successfully inhibited the scratch assay closure and inflammatory abnormalities in the commercially available keloid fibroblast cell line. Caffeine and allicin also impacted ATP production in keloid cells, most notably with inhibition of non-mitochondrial oxygen consumption. The traditional Chinese medicine, shikonin, was also successful in inhibiting scratch closure but displayed less dramatic impacts on metabolism. Together, our results partially summarize the strengths and limitations of current scratch assay literature and suggest clinical assessment of the therapeutic potential for these identified compounds against keloid scars may be warranted.

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Jana, Snehasis y Mahendra Kumar Trivedi. "Wound Healing Activity of Consciousness Energy Healing Treatmenton HFF-1 Cells and DMEM Using Scratch Assay". Investigative Dermatology and Venereology Research 4, n.º 1 (26 de diciembre de 2018): 50–54. http://dx.doi.org/10.15436/2381-0858.18.2036.

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The wound healing activity using scratch assay is considered as a convenient in vitro tool for the assessment of wound healing. The present study deals with the optimization of Biofield Energy Treatment (Consciousness Energy Healing Treatment-The Trivedi Effect®) in the HFF-1 cell line (Human Foreskin Fibroblast) and DMEM (Dulbecco's Modified Eagle Medium) using scratch assay against positive control, recombinant Human Epidermal Growth Factor (Hu EGF, 30 ng/mL). This methodwas used for the determination of cell proliferation and migration of fibroblast quantitatively in the scratched wounded area. The scratched area was monitored after 24 hours of wound closure in the Biofield Energy Treated HFF-1 cells and the Biofield Energy Treated DMEM groups, and the representative photomicrographs were taken in each wells using WimScratch Image analysis software. The results showed that the Biofield Energy Treated DMEMwassignificantly higher percentage of fibroblast migration i.e. 51.8%, while the migration was altered in the Biofield Energy Treated HFF-1 cell line compared to the baseline control group. In addition to, the percentage of scratch area was significantly decreased by 2.7% in the Biofield Energy Treated DMEM group, while it was increased by 12% in the Biofield Treated cells group compared to the baseline control group. Overall, the experimental results concluded that The Trivedi Effect® has the significant capacity and wide implications in wound healing activityvia cell culture media, DMEM as compared with the HFF-1 cell line directly. Biofield Energy Healing would be a complementary and alternative medicine that can be used against burn injury cases, acute wound, skin regeneration, eczema, diaper rash, chickenpox, measles, warts, acne, hives, wrinkles, ringworm, Rosacea, psoriasis, seborrheic dermatitis, skin cancer, etc.

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Freiesleben, Sara H., Jens Soelberg, Nils T. Nyberg y Anna K. Jäger. "Determination of the Wound Healing Potentials of Medicinal Plants Historically Used in Ghana". Evidence-Based Complementary and Alternative Medicine 2017 (2017): 1–6. http://dx.doi.org/10.1155/2017/9480791.

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The present study was carried out to investigate the wound healing potentials of 17 medicinal plants historically used in Ghana for wound healing. Warm and cold water extracts were prepared from the 17 dried plant species and tested in vitro in the scratch assay with NIH 3T3 fibroblasts from mice. The wound healing scratch assay was used to evaluate the effect of the plants on cell proliferation and/or migration in vitro, as a test for potential wound healing properties. After 21 hours of incubation increased proliferation and/or migration of fibroblasts in the scratch assay was obtained for 5 out of the 17 plant species. HPLC separation of the most active plant extract, which was a warm water extract of Philenoptera cyanescens, revealed the wound healing activity to be attributed to rutin and a triglycoside of quercetin. The present study suggests that Allophylus spicatus, Philenoptera cyanescens, Melanthera scandens, Ocimum gratissimum, and Jasminum dichotomum have wound healing activity in vitro.

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Saha, Susmita, Deepjyoti Bhattacharjee, Anwesha Saha, Gahin De, Partha Saha y S. K. Sil. "Wound healing promoting activity of Earthworm, Eutyphoeus gammiei (Beddard): in vitro studies on human skin keratinocyte cell line (HaCat)." Journal of Drug Delivery and Therapeutics 8, n.º 6 (15 de noviembre de 2018): 155–58. http://dx.doi.org/10.22270/jddt.v8i6.2036.

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Earthworm, Eutyphoeus gammiei, homogenate (EGH) was screened for wound healing activity on human keratinocyte cell line, HaCat, by cell proliferation and migration assays. The maximum proliferation and migration of keratinocyte cells were observed at the dose of 25μg/ml. As cell proliferation and migration are key factors for wound healing, the study clearly suggests the potential role of earthworm species Eutyphoeus gammiei on wound healing. Keywords: Eutyphoeus gammiei, Keratinocyte, MTT assay, scratch assay.

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Suriyah, Wastuti Hidayati, Aisyah Juares Rizal, Hana Syakirah Mohamed Nadzirin, Solachuddin Jauhari Arief Ichwan y Muhammad Lokman Md Isa. "In Vitro Wound Healing Effect of Asiaticoside Extracted from Centella asiatica (‘Pegaga’) on Human Gingival Fibroblast Cell Line". Materials Science Forum 1025 (marzo de 2021): 224–29. http://dx.doi.org/10.4028/www.scientific.net/msf.1025.224.

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Asiaticoside is a bioactive compound found in the traditional plant Centella asiatica (Asiatic pennywort or ‘Pegaga’) generally used for wound healing applications. Numerous studies have discussed the potential benefits of asiaticoside on different human cells such as keratinocytes and dermal fibroblast cells in healing of wounds. However only very few studies have been conducted to investigate its healing effect on cells originated from human oral cavity. The present study aimed to determine the potential of asiaticoside on human gingival fibroblast cells. Cytotoxic activities of the compounds were assessed by MTT assay. The wound healing was examined by scratch assay. The effect of asiaticoside on Col1A1 gene expression was also analyzed using qRT-PCR. Col1A1 is known to play a crucial role in wound healing. The MTT assay result showed that the maximum tolerable concentration of asiaticoside was 0.25 mg/ml. The scratch assay revealed that asiaticoside significantly accelerated the wound healing compared to the negative control (P<0.05). Moreover, the qRT-PCR demonstrated that asiaticoside markedly increased Col1A1 mRNA expression. These results proved asiaticoside as a potential candidate for wound healing agent in dentistry.

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Wu, Shang-Ying, Yung-Shin Sun, Kuan-Chen Cheng y Kai-Yin Lo. "A Wound-Healing Assay Based on Ultraviolet Light Ablation". SLAS TECHNOLOGY: Translating Life Sciences Innovation 22, n.º 1 (10 de julio de 2016): 36–43. http://dx.doi.org/10.1177/2211068216646741.

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Collective cell migration plays important roles in many physiological processes such as embryonic development, tissue repair, and angiogenesis. A “wound” occurs when epithelial cells are lost and/or damaged due to some external factors, and collective cell migration takes place in the following wound-healing process. To study this cellular behavior, various kinds of wound-healing assays are developed. In these assays, a “wound,” or a “cell-free region,” is created in a cell monolayer mechanically, chemically, optically, or electrically. These assays are useful tools in studying the effects of certain physical or chemical stimuli on the wound-healing process. Most of these methods have disadvantages such as creating wounds of different sizes or shapes, yielding batch-to-batch variation, and damaging the coating of the cell culture surface. In this study, we used ultraviolet (UV) lights to selectively kill cells and create a wound out of a cell monolayer. A comparison between the current assay and the traditional scratch assay was made, indicating that these two methods resulted in similar wound-healing rates. The advantages of this UV-created wound-healing assay include fast and easy procedure, high throughput, and no direct contact to cells.

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Abbas, ManalAhmad, ManalMohammad Abbas, Naseer Al-Rawi y Iqbal Al-Khateeb. "Naringenin potentiated β-sitosterol healing effect on the scratch wound assay". Research in Pharmaceutical Sciences 14, n.º 6 (2019): 566. http://dx.doi.org/10.4103/1735-5362.272565.

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Choi, Sun-Hye, Kyung-Jong Won, Rami Lee, Han-Sung Cho, Sung-Hee Hwang y Seung-Yeol Nah. "Wound Healing Effect of Gintonin Involves Lysophosphatidic Acid Receptor/Vascular Endothelial Growth Factor Signaling Pathway in Keratinocytes". International Journal of Molecular Sciences 22, n.º 18 (21 de septiembre de 2021): 10155. http://dx.doi.org/10.3390/ijms221810155.

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Gintonin, a novel compound of ginseng, is a ligand of the lysophosphatidic acid (LPA) receptor. The in vitro and in vivo skin wound healing effects of gintonin remain unknown. Therefore, the objective of this study was to investigate the effects of gintonin on wound healing-linked responses, especially migration and proliferation, in skin keratinocytes HaCaT. In this study, 2,3-bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide assay, Boyden chamber migration assay, scratch wound healing assay, and Western blot assay were performed. A tail wound mouse model was used for the in vivo test. Gintonin increased proliferation, migration, and scratch closure in HaCaT cells. It also increased the release of vascular endothelial growth factor (VEGF) in HaCaT cells. However, these increases, induced by gintonin, were markedly blocked by treatment with Ki16425, an LPA inhibitor, PD98059, an ERK inhibitor, 1,2-Bis(2-aminophenoxy)ethane-N,N,N′,N′-tetraacetic acid tetrakis (acetoxymethyl ester), a calcium chelator, and U73122, a PLC inhibitor. The VEGF receptor inhibitor axitinib also attenuated gintonin-enhanced HaCaT cell proliferation. Gintonin increased the phosphorylation of AKT and ERK1/2 in HaCaT cells. In addition, gintonin improved tail wound healing in mice. These results indicate that gintonin may promote wound healing through LPA receptor activation and/or VEGF release-mediated downstream signaling pathways. Thus, gintonin could be a beneficial substance to facilitate skin wound healing.

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Dhillon, Prabhpreet K., Xinyin Li, Jurgen T. Sanes, Oluwafemi S. Akintola y Bingyun Sun. "Method comparison for analyzing wound healing rates". Biochemistry and Cell Biology 95, n.º 3 (junio de 2017): 450–54. http://dx.doi.org/10.1139/bcb-2016-0163.

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Wound healing scratch assay is a frequently used method to characterize cell migration, which is an important biological process in the course of development, tissue repair, and immune response for example. The measurement of wound healing rate, however, varies among different studies. Here we summarized these measurements into three types: (I) direct rate average; (II) regression rate average; and (III) average distance regression rate. Using Chinese hamster ovary (CHO) cells as a model, we compared the three types of analyses on quantifying the wound closing rate, and discovered that type I & III measurements are more resistant to outliers, and type II analysis is more sensitive to outliers. We hope this study can help researchers to better use this simple yet effective assay.

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Bakari, G. G., S. A. Mshamu, M. H. Ally, R. A. Max y H. Bai. "In-vitro Wound Healing Properties of Commiphora swynnertonii Resinous Extracts". Tanzania Veterinary Journal 38 (4 de septiembre de 2021): 32–37. http://dx.doi.org/10.4314/tvj.v38i1.6s.

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Wound healing is a complex multicellular process involving many cell types which include; inflammatory cells, endothelial cells, fibroblasts and keratinocytes. The process involves an orderly sequence of events with four overlapping phases namely; haemostasis, inflammatory, proliferation and remodeling phases. The process can be facilitated by the use of wound healing agents including herbal remedies from plants. In this study the main objective was to evaluate the in vitro wound healing activity of the resin obtained from Commiphora swynnertonii (C.swynnertonii). First the NIH -3T3 cells viability were evaluated using (3-(4, 5-Dimethylthiazol-2-yl)-2, 5-diphenyl Tetrazolium Bromide (MTT) assay. Then the wound scratch assay model was used to evaluate cellular proliferation, closure of the wound and release of matrix metalloproteinase enzymes. Results indicate differences in mean cell viability between different concentrations within 24 hours of incubation. The highest viability was recorded at the concentration of 1% (v/v). The in-vitro wound scratch assay showed positive NIH - 3T3 cells proliferation on the wound area and cells migration when compared with control group (without treatment) at 0 and 24 hours. In addition, C. swynnertonii was able to stimulate secretion of MMP-2 release from NIH - 3T3 cells. MMP-2 is an important enzyme for extracellular matrix remodeling during wound healing suggesting that C. swynnertonii promotes wound healing by stimulating cell proliferation and production of MMP-2 in a mechanism that is currently not known.

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Más fuentes

Tesis sobre el tema "Scratch Wound Healing Assay"

Morgaenko, Katsiarina. "Sledování migrace buněk v mikrofluidním systému metodou „Scratch Wound Healing Assay“". Master's thesis, Vysoké učení technické v Brně. Fakulta elektrotechniky a komunikačních technologií, 2019. http://www.nusl.cz/ntk/nusl-403757.

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Tato diplomová práce se zabývá popisem principů kultivace embryonálních fibroblastových buněk myší (3T3), lidských endoteliálních buněk odebraných z pupečníkové žily (HUVEC) a epiteliálních buněk vaječníku čínského křečka (CHO) v mikrofluidních systémech simulujících kapiláry. Byly provedeny literární rešerše v oblasti realizací experimentu “Scratch Wound Healing Assay” v mikrofluidních systémech s použitím fibroblastů a endotheliálních buněk. V práci jsou dále popsány principy konfokální a fluorescenční mikroskopie a metody zpracování obrazů pro sledování buněčné migrace. Experimentální nastavení pro mikrofluidní realizaci “Scratch Wound Healing Assay” s použitím trypsinu – EDTA pro vytvoření rýhy, a konfokálního mikroskopu Leica TCS SP8 X pro následující snímání pořízených dat bylo navrženo a otestováno s dostatečným počtem opakování. Vhodný algoritmus pro analýzu buněčné migrace byl napsán v programovacím prostředí Matlab. Závěrem této práce je diskuze získaných výsledků.

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Alsabri, Sami Gamaleddin F. "Usage of Extracellular Microvesicles as Novel and Promising Therapeutic Tool in Wound Healing". Wright State University / OhioLINK, 2017. http://rave.ohiolink.edu/etdc/view?acc_num=wright1512717040231595.

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Yang, Yongliang. "Emergent Leader Cells in Collective Cell Migration in In Vitro Wound Healing Assay". Diss., The University of Arizona, 2014. http://hdl.handle.net/10150/332896.

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Collective cell migration is critical for various physiological and pathological processes. In vitro wound healing assay has been widely used to study collective cell migration due to its technical simplicity and ability of revealing the complexity of collective cell migration. This project studies the function and importance of leader cells, the cells pulling cell monolayer migrating into free space, in endothelium and skin epithelial regeneration via plasma lithography enhanced in vitro wound healing assay. Despite leader cells have been identified in in vitro wound healing assays, little is known about their regulation and function on collective cell migration. First, I investigated the role of leader cells in endothelial cell collective migration. I found that the leader cell density is positively related with the cell monolayer migration rates. Second, we used this knowledge to study the effects of arsenic treatment on skin regeneration via in vitro wound healing assay. We found that low concentration of arsenic treatment can accelerate the keratinocyte monolayer migration. We further found that arsenic affected cell migration by modulating leader cell density through Nrf2 signaling pathway. As a conclusion of these studies, we evaluated the function of leader cells in collective cell migration, and elucidated the mechanism of arsenic treatment on skin regeneration.

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Post, Hannah [Verfasser] y Jennifer E. [Akademischer Betreuer] Hundt. "Development and testing of a novel ex vivo assay for studying “pathological” wound healing in human skin / Hannah Post ; Akademischer Betreuer: Jennifer E. Hundt". Lübeck : Zentrale Hochschulbibliothek Lübeck, 2021. http://d-nb.info/1227903251/34.

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Mun, Kyu-Shik. "Monitoring Cell Behaviors on Variety of Micropatterns Created with Biodegradable Polymer". University of Cincinnati / OhioLINK, 2016. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1457426363.

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Pillai, Mahesh Ramachandran. "Deciphering the Link Between Polychlorinated Biphenyls, Immune Function and Exercise". Bowling Green State University / OhioLINK, 2017. http://rave.ohiolink.edu/etdc/view?acc_num=bgsu1510140839084446.

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Yeh, Chun Chih y 葉軍志. "Three-dimensional wound healing assay". Thesis, 2019. http://ndltd.ncl.edu.tw/cgi-bin/gs32/gsweb.cgi/login?o=dnclcdr&s=id=%22107CGU05114064%22.&searchmode=basic.

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Peng, Shih-Wei y 彭士瑋. "A modified wound-healing-assay chip for studying electric field-assisted wound healing process". Thesis, 2012. http://ndltd.ncl.edu.tw/handle/75087010920609013295.

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碩士
國立陽明大學
生醫光電研究所
100
There are several wound healing assays based on scratching, solid barrier and liquid barrier. However, none of them can represent the actual micro-environment which represents the direction of flow and EF toward the center of the wound. It has been suggested that wound healing is related to electric fields. Recently, Min Zhao et al. found the electrical signal also regulates the wound re-epithelialization. The disruption of epithelial barrier short-circuits the trans-epithelial potential and then creates a lateral endogenous electric field. The field has already been proofed as an important cue for guiding the migration direction of the fibroblasts, macrophages and keratinocytes in response to wounding site of a monolayer in vitro. This induced directional movement of cells toward the cathode or the anode under direct current electric field is so called electrotaxis. In this abstract, we propose a modified wound-healing-assay chip for studying electric field-assisted wound healing process. In preliminary test, we adopt NIH/3T3 fibroblast cell line to demonstrate the feasibility of our chip.

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Chiang, Pei-Shuan y 江旆萱. "In Vitro wound healing assay revisited: aided by a long-term, time-lapse recording system". Thesis, 2003. http://ndltd.ncl.edu.tw/handle/61577690168058185073.

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碩士
國立成功大學
分子醫學研究所
91
Wound healing is one of the most frequently used methods to study cell motility. A monolayer of cells is scratch-wounded and cells alongside the wound would proliferate and migrate to fill up the denuded area. The area change or the wound closure distance is considered to be a measurement of cell motility. However, the rate of wound closure may not be a true measurement of cell motility. By measuring the distance of wound closure, we found that high-density monolayers of T24 cells (a bladder cancer cell line) showed faster wound-closure rates than low-density ones, which, by conventional interpretation, implied that T24 cells at higher cell densities would have greater cell motilities. To clarify such an observation, we investigated the details of wound healing with our long-term, time-lapse recording system, which was able to record and depict the migration path of a single cell through the entire healing process. Only the first few rows of cells behind the wounded edge contributed to wound closure. These cells showed better moving directionality (toward the direction of wound closure) at higher cell-densities, explaining the greater wound-closure rate, whereas the average lengths of the migration paths are the same in high- and low-density experiments. The lengths of migration paths over a period of time are the better measurement of cell motility, whereas the wound-closure rate represents the combinational effect of cell motility and directionality. The effects of mitomycin C and β-Glycyrrhetinic acid on wound-closure rate and cell motility in wound healing were further investigated. It had been suggested that in order to minimize the effect of cell proliferation on wound healing, the proliferation activity should be inhibited or the assay time should be kept as short as possible. However, we found that inhibition of cell proliferation by mitomycin C treatment may affect cell motility in a short period of time. Gap junctional communication was thought to play a role in wound healing. By inhibition of gap junction with GCA, we found that the percentage of forward moving cells as well as the migration rate significantly decreased. In the second part of the study, we applied the long-term time-lapse recording system to the functional analyses of genes through transient transfections. A preliminary procedure was established. Using a construct to co-express green fluorescent protein and EMP2 in NIH3T3 cells, we established a procedure to evaluate potential effects of EMP2 on cell morphology, viability, apoptosis, membrane ruffling and cell motility. The procedure could be utilized as a rapid screening test for gene functions.

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Nasir, N. A. M., R. Paus y David M. Ansell. "Fluorescent cell tracer dye permits real-time assessment of re-epithelialization in a serum-free ex vivo human skin wound assay". 2018. http://hdl.handle.net/10454/17786.

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Yes
Ex vivo wounded human skin organ culture is an invaluable tool for translationally relevant preclinical wound healing research. However, studies incorporating this system are still underutilized within the field because of the low throughput of histological analysis required for downstream assessment. In this study, we use intravital fluorescent dye to lineage trace epidermal cells, demonstrating that wound re‐epithelialization of human ex vivo wounds occurs consistent with an extending shield mechanism of collective migration. Moreover, we also report a relatively simple method to investigate global epithelial closure of explants in culture using daily fluorescent dye treatment and en face imaging. This study is the first to quantify healing of ex vivo wounds in a longitudinal manner, providing global assessments for re‐epithelialization and tissue contraction. We show that this approach can identify alterations to healing with a known healing promoter. This methodological study highlights the utility of human ex vivo wounds in enhancing our understanding of mechanisms of human skin repair and in evaluating novel therapies to improve healing outcome.
University of Manchester Strategic Fund; Wellcome Trust; BBSRC; Ministry of Higher Education, Malaysia Universiti; Sains Malaysia

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Capítulos de libros sobre el tema "Scratch Wound Healing Assay"

Martinotti, Simona y Elia Ranzato. "Scratch Wound Healing Assay". En Methods in Molecular Biology, 225–29. New York, NY: Springer US, 2019. http://dx.doi.org/10.1007/7651_2019_259.

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Cory, Giles. "Scratch-Wound Assay". En Methods in Molecular Biology, 25–30. Totowa, NJ: Humana Press, 2011. http://dx.doi.org/10.1007/978-1-61779-207-6_2.

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Freitas, Juliano T., Ivan Jozic y Barbara Bedogni. "Wound Healing Assay for Melanoma Cell Migration". En Methods in Molecular Biology, 65–71. New York, NY: Springer US, 2021. http://dx.doi.org/10.1007/978-1-0716-1205-7_4.

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Ganguli-Indra, Gitali. "Protocol for Cutaneous Wound Healing Assay in a Murine Model". En Stem Cells and Tissue Repair, 151–59. New York, NY: Springer New York, 2014. http://dx.doi.org/10.1007/978-1-4939-1435-7_12.

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Zemkewicz, John L., Racheal G. Akwii, Constantinos M. Mikelis y Colleen L. Doçi. "Investigating Epidermal Interactions Through an In Vivo Cutaneous Wound-Healing Assay". En Methods in Molecular Biology, 1–11. New York, NY: Springer US, 2020. http://dx.doi.org/10.1007/978-1-0716-0845-6_1.

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Cardona, A., L. Ariza-Jiménez, D. Uribe, J. Arroyave y F. M. Cortés-Mancera. "Automatic Image Segmentation Method for In Vitro Wound Healing Assay Quantitative Analysis". En VI Latin American Congress on Biomedical Engineering CLAIB 2014, Paraná, Argentina 29, 30 & 31 October 2014, 381–84. Cham: Springer International Publishing, 2015. http://dx.doi.org/10.1007/978-3-319-13117-7_98.

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Tong, Junfeng y Zhixiang Wang. "Analysis of Epidermal Growth Factor Receptor-Induced Cell Motility by Wound Healing Assay". En Methods in Molecular Biology, 159–63. New York, NY: Springer New York, 2017. http://dx.doi.org/10.1007/978-1-4939-7219-7_12.

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Castellano-Pellicena, Irene y M. Julie Thornton. "Isolation of Epidermal Keratinocytes from Human Skin: The Scratch-Wound Assay for Assessment of Epidermal Keratinocyte Migration". En Methods in Molecular Biology, 1–12. New York, NY: Springer US, 2020. http://dx.doi.org/10.1007/978-1-0716-0648-3_1.

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"Wound Healing Assay". En Cellular Potts Models, 89–102. Chapman and Hall/CRC, 2013. http://dx.doi.org/10.1201/b14075-9.

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"Wound Healing Assay". En Encyclopedia of Cancer, 3958. Berlin, Heidelberg: Springer Berlin Heidelberg, 2011. http://dx.doi.org/10.1007/978-3-642-16483-5_6262.

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Actas de conferencias sobre el tema "Scratch Wound Healing Assay"

Cohen Maslaton, Shir y Natan T. Shaked. "Wound healing assay of two competing cell types with dry mass measurement". En Optical Methods for Inspection, Characterization, and Imaging of Biomaterials IV, editado por Pietro Ferraro, Monika Ritsch-Marte, Simonetta Grilli y Christoph K. Hitzenberger. SPIE, 2019. http://dx.doi.org/10.1117/12.2526841.

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Wei, Y., F. Chen, T. Zhang, D. Chen, X. Jia, J. Tong, J. Wang, W. Guo y J. Chen. "A tubing-free microfluidic wound-healing assay quantifying vascular smooth muscle cell migration". En TRANSDUCERS 2015 - 2015 18th International Solid-State Sensors, Actuators and Microsystems Conference. IEEE, 2015. http://dx.doi.org/10.1109/transducers.2015.7181291.

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Bise, R., T. Kanade, Zhaozheng Yin y Seung-il Huh. "Automatic cell tracking applied to analysis of cell migration in wound healing assay". En 2011 33rd Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE, 2011. http://dx.doi.org/10.1109/iembs.2011.6091525.

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Mondal, N., D. Mondal, C. RoyChaudhuri, A. Barui, S. Dhara y J. Chatterjee. "A simple and sensitive cytosensor based electrical characterization of in vitro wound healing assay for keratinocytes". En 2011 IEEE/NIH 5th Life Science Systems and Applications Workshop (LiSSA). IEEE, 2011. http://dx.doi.org/10.1109/lissa.2011.5754152.

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Topman, Gil, Orna Sharabani-Yosef y Amit Gefen. "A Method for Quantitative Analysis of the Kinematics of Fibroblast Migration in a Monolayer Wound Model". En ASME 2011 Summer Bioengineering Conference. American Society of Mechanical Engineers, 2011. http://dx.doi.org/10.1115/sbc2011-53070.

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A wound healing assay is simple but effective method to study cell migration in vitro. Cell migration in vitro was found to mimic migration in vivo to some extent [1,2]. In wound healing assays, a “wound” is created by either scraping or mechanically crushing cells in a monolayer, thereby forming a denuded area. Cells migrate into the denuded area to complete coverage, and thereby “heal” the wound. Micrographs at regular time intervals are captured during such experiments for analysis of the process of migration.

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Galeano Zea, July A., Cesar Bedoya, Cardona Andrés, Fabián Cortés-Mancera, Patrick Sandoz y Artur Zarzycki. "Modified position-referenced microscopy for the analysis of low-magnification biological events: a case of study in the wound healing assay with a human hepatoma cell line". En Latin America Optics and Photonics Conference. Washington, D.C.: OSA, 2016. http://dx.doi.org/10.1364/laop.2016.ltu4a.52.

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Maistrenko, Lesia, Olga Iungin, Oleksii Savchuk y Olena Okhmat. "Collagen matrices from leather industry wastes for biomedical application". En The 8th International Conference on Advanced Materials and Systems. INCDTP - Leather and Footwear Research Institute (ICPI), Bucharest, Romania, 2020. http://dx.doi.org/10.24264/icams-2020.ii.15.

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Modern biomedical science is challenged to develop new wound healing drugs. The collagen-containing wastes of leather industry could be the rich source of collagen products for further use in biomedical science. The aim of this research was to find the best source of collagen between limed pelt, delimed pelt and fleshings of cattle hides, and to prepare it for the use as a matrix for further microbiological studies. Collagen was extracted with 0.5 M acetic acid and 5 mM EDTA. The purity of the extracted collagen was checked by gel-electophoresis (SDS-PAGE). The rate of growth and crystal violet assay of laboratory strains (S. aureus, P. aeruginosa) were used for microbiological evaluation of obtained collagen matrices. The delimed pelt provided the highest concentration of collagen and the greatest volume of collagen products. All obtained collagen products were applicable as matrices for microbial cells growth. The applicability of collagen products from leather industry wastes for biomedical studies in Ukraine was shown.

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