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Artículos de revistas sobre el tema "Sepsityper"

Autor: Grafiati
Publicado: 4 de junio de 2021
Última modificación: 10 de febrero de 2022

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1

Arroyo, Miguel A., and Gerald A. Denys. "Parallel Evaluation of the MALDI Sepsityper and Verigene BC-GN Assays for Rapid Identification of Gram-Negative Bacilli from Positive Blood Cultures." Journal of Clinical Microbiology 55, no. 9 (2017): 2708–18. http://dx.doi.org/10.1128/jcm.00692-17.

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ABSTRACT Rapid identification of microorganisms from positive blood cultures has improved clinical management and antimicrobial stewardship. The advent of matrix-assisted laser desorption ionization–time of flight mass spectrometry (MALDI-TOF MS) has reduced the time to identification of cultured isolates and is now often the definitive method used in the clinical microbiology laboratory. The commercial in vitro diagnostic MALDI Sepsityper (Sepsityper) kit has the potential for standardization and clinical routine use for the rapid identification of a broad range of bacteria from positive bloo
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2

Cordovana, Miriam, Anna Zignoli, and Simone Ambretti. "Rapid Sepsityper in clinical routine: 2 years’ successful experience." Journal of Medical Microbiology 69, no. 12 (2020): 1398–404. http://dx.doi.org/10.1099/jmm.0.001268.

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Introduction. Rapid identification of the causative agent of sepsis is crucial for patient outcomes. Aim. The Sepsityper sample preparation method enables direct microbial identification of positive blood culture samples via matrix-assisted laser desorption ionization/time-of-flight mass spectrometry (MALDI-TOF MS). Hypothesis/Gap statement. The implementation of the Sepsityper method in the routine practice could represent a fundamental tool to achieve a prompt identification of the causative agent of bloodstream infections, and therefore accelerate the adoption of the proper antibiotic treat
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3

Morgenthaler, Nils G., and Markus Kostrzewa. "Rapid Identification of Pathogens in Positive Blood Culture of Patients with Sepsis: Review and Meta-Analysis of the Performance of the Sepsityper Kit." International Journal of Microbiology 2015 (2015): 1–10. http://dx.doi.org/10.1155/2015/827416.

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Sepsis is one of the leading causes of deaths, and rapid identification (ID) of blood stream infection is mandatory to perform adequate antibiotic therapy. The advent of MALDI-TOF Mass Spectrometry for the rapid ID of pathogens was a major breakthrough in microbiology. Recently, this method was combined with extraction methods for pathogens directly from positive blood cultures. This review summarizes the results obtained so far with the commercial Sepsityper sample preparation kit, which is now approved forin vitrodiagnostic use. Summarizing data from 21 reports, the Sepsityper kit allowed a
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4

Noll, Christine, Azadda Nasruddin-Yekta, Pia Sternisek, et al. "Rapid direct detection of pathogens for diagnosis of joint infections by MALDI-TOF MS after liquid enrichment in the BacT/Alert blood culture system." PLOS ONE 15, no. 12 (2020): e0243790. http://dx.doi.org/10.1371/journal.pone.0243790.

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Pathogen identification is a critical step during diagnosis of infectious diseases. Matrix-Assisted Laser Desorption/Ionization Time-Of-Flight mass spectrometry (MALDI-TOF-MS) has become the gold standard for identification of microorganisms cultured on solid media in microbiology laboratories. Direct identification of microbes from liquid specimen, circumventing the need for the additional overnight cultivation step, has been successfully established for blood culture, urine and liquor. Here, we evaluate the ability of MALDI-TOF MS for direct identification of pathogens in synovial fluid afte
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5

Camacho-Luque, Raquel, Alejandro Peña-Monje, Marta Álvarez-Estévez, et al. "Sepsityper® for rapid identification of microorganisms from positive blood cultures." ACTUALIDAD MEDICA 99, no. 792 (2014): 70–74. http://dx.doi.org/10.15568/am.2014.792.or03.

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6

Hazelton, Briony, Lee C. Thomas, Thomas Olma, et al. "Rapid and accurate direct antibiotic susceptibility testing of blood culture broths using MALDI Sepsityper combined with the BD Phoenix automated system." Journal of Medical Microbiology 63, no. 12 (2014): 1590–94. http://dx.doi.org/10.1099/jmm.0.075580-0.

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Antibiotic susceptibility testing with the BD Phoenix system on bacterial cell pellets generated from blood culture broths using the Bruker MALDI Sepsityper kit was evaluated. Seventy-six Gram-negative isolates, including 12 with defined multi-resistant phenotypes, had antibiotic susceptibility testing (AST) performed by Phoenix on the cell pellet in parallel with conventional methods. In total, 1414/1444 (97.9 %) of susceptibility tests were concordant, with only 1 (0.07 %) very major error. This novel method has the potential to reduce the turnaround time for AST results by up to a day for G
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7

Enroth, Helena, Karolina Retz, Sofie Andersson, et al. "Evaluation of QuickFISH and maldi Sepsityper for identification of bacteria in bloodstream infection." Infectious Diseases 51, no. 4 (2019): 249–58. http://dx.doi.org/10.1080/23744235.2018.1554258.

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8

Kayin, Munevver, Berivan Mert, Söhret Aydemir, and Volkan Özenci. "Comparison of rapid BACpro® II, Sepsityper® kit and in-house preparation methods for direct identification of bacteria from blood cultures by MALDI-TOF MS with and without Sepsityper® module analysis." European Journal of Clinical Microbiology & Infectious Diseases 38, no. 11 (2019): 2133–43. http://dx.doi.org/10.1007/s10096-019-03654-4.

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9

Tadros, Manal, and Astrid Petrich. "Evaluation of MALDI-TOF Mass Spectrometry and Sepsityper Kit™ for the Direct Identification of Organisms from Sterile Body Fluids in a Canadian Pediatric Hospital." Canadian Journal of Infectious Diseases and Medical Microbiology 24, no. 4 (2013): 191–94. http://dx.doi.org/10.1155/2013/701093.

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Matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF MS) can be used to identify bacteria directly from positive blood and sterile fluid cultures. The authors evaluated a commercially available kit – the Sepsityper Kit (Bruker Daltonik, Germany) – and MALDI-TOF MS for the rapid identification of organisms from 80 flagged positive blood culture broths, of which 73 (91.2%) were blood culture specimens and seven (8.7%) were cerebrospinal fluid specimens, in comparison with conventional identification methods. Correct identification to the genus and species level
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10

Szultka-Młyńska, Małgorzata, Daria Janiszewska, Paweł Pomastowski, Michał Złoch, Wojciech Kupczyk, and Bogusław Buszewski. "Identification of Bacteria Associated with Post-Operative Wounds of Patients with the Use of Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry Approach." Molecules 26, no. 16 (2021): 5007. http://dx.doi.org/10.3390/molecules26165007.

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The bacterial infection of post-operative wounds is a common health problem. Therefore, it is important to investigate fast and accurate methods of identifying bacteria in clinical samples. The aim of the study was to analyse the use of the MALDI-TOF MS technique to identify microorganism wounds that are difficult to heal. The most common bacteria are Escherichia coli, Staphylococcus spp., and Enterococcus spp. We also demonstrate the effect of culture conditions, such as the used growth medium (solid: Brain Heart Infusion Agar, Mueller Hilton Agar, Glucose Bromocresol Purple Agar, and Vancomy
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11

Bal, AM, and M. McGill. "Rapid species identification of Candida directly from blood culture broths by Sepsityper-MALDI-TOF mass spectrometry: impact on antifungal therapy." Journal of the Royal College of Physicians of Edinburgh 48, no. 2 (2018): 114–19. http://dx.doi.org/10.4997/jrcpe.2018.203.

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12

Di Gaudio, Francesca, Serena Indelicato, Sergio Indelicato, Maria Rita Tricoli, Giuseppe Stampone, and David Bongiorno. "Improvement of a rapid direct blood culture microbial identification protocol using MALDI-TOF MS and performance comparison with SepsiTyper kit." Journal of Microbiological Methods 155 (December 2018): 1–7. http://dx.doi.org/10.1016/j.mimet.2018.10.015.

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13

Scohy, Anaïs, Audrey Noël, Anca Boeras, et al. "Evaluation of the Bruker® MBT Sepsityper IVD module for the identification of polymicrobial blood cultures with MALDI-TOF MS." European Journal of Clinical Microbiology & Infectious Diseases 37, no. 11 (2018): 2145–52. http://dx.doi.org/10.1007/s10096-018-3351-2.

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14

Fang, Chao, Zheng Zhou, Jianping Li, Xuejun Chen, and Mingming Zhou. "Rapid identification of microorganisms from positive blood cultures in pediatric patients by MALDI-TOF MS: Sepsityper kit versus short-term subculture." Journal of Microbiological Methods 172 (May 2020): 105894. http://dx.doi.org/10.1016/j.mimet.2020.105894.

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15

Kok, Jen, Lee C. Thomas, Thomas Olma, Sharon C. A. Chen, and Jonathan R. Iredell. "Identification of Bacteria in Blood Culture Broths Using Matrix-Assisted Laser Desorption-Ionization Sepsityper™ and Time of Flight Mass Spectrometry." PLoS ONE 6, no. 8 (2011): e23285. http://dx.doi.org/10.1371/journal.pone.0023285.

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16

Riederer, Kathleen, Kristian Cruz, Stephen Shemes, Susan Szpunar, and Joel T. Fishbain. "MALDI-TOF identification of Gram-negative bacteria directly from blood culture bottles containing charcoal: Sepsityper® kits versus centrifugation–filtration method." Diagnostic Microbiology and Infectious Disease 82, no. 2 (2015): 105–8. http://dx.doi.org/10.1016/j.diagmicrobio.2015.03.003.

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17

Buchan, B. W., K. M. Riebe, and N. A. Ledeboer. "Comparison of the MALDI Biotyper System Using Sepsityper Specimen Processing to Routine Microbiological Methods for Identification of Bacteria from Positive Blood Culture Bottles." Journal of Clinical Microbiology 50, no. 2 (2011): 346–52. http://dx.doi.org/10.1128/jcm.05021-11.

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18

Gorton, Rebecca L., P. Ramnarain, K. Barker, et al. "Comparative analysis of Gram's stain, PNA-FISH and Sepsityper with MALDI-TOF MS for the identification of yeast direct from positive blood cultures." Mycoses 57, no. 10 (2014): 592–601. http://dx.doi.org/10.1111/myc.12205.

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19

Delport, J. A., M. John, B. Conkey, K. Macdonald, and R. Lannigan. "Comparison of direct identification of bacteria from VersaTrek Blood culture bottles using a seven present saponin extraction method and SepsiTyper by the Bruker MALDI-TOF." International Journal of Infectious Diseases 21 (April 2014): 371. http://dx.doi.org/10.1016/j.ijid.2014.03.1186.

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20

Fre´de´ric, S., M. Antoine, A. Bodson, and B. Lissoir. "Bacterial rapid identification with matrix assisted laser desorption/ionization time-of-flight mass spectrometry: development of an ‘in-house method’ and comparison with Bruker Sepsityper®kit." Acta Clinica Belgica 70, no. 5 (2015): 325–30. http://dx.doi.org/10.1179/2295333715y.0000000026.

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21

Meex, Cécile, Florence Neuville, Julie Descy, et al. "Direct identification of bacteria from BacT/ALERT anaerobic positive blood cultures by MALDI-TOF MS: MALDI Sepsityper kit versus an in-house saponin method for bacterial extraction." Journal of Medical Microbiology 61, no. 11 (2012): 1511–16. http://dx.doi.org/10.1099/jmm.0.044750-0.

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22

Yan, Yingjun, Ying He, Thomas Maier, et al. "Improved Identification of Yeast Species Directly from Positive Blood Culture Media by Combining Sepsityper Specimen Processing and Microflex Analysis with the Matrix-Assisted Laser Desorption Ionization Biotyper System." Journal of Clinical Microbiology 49, no. 7 (2011): 2528–32. http://dx.doi.org/10.1128/jcm.00339-11.

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23

Bidart, Marie, Isabelle Bonnet, Aurélie Hennebique, et al. "An In-House Assay Is Superior to Sepsityper for Direct Matrix-Assisted Laser Desorption Ionization–Time of Flight (MALDI-TOF) Mass Spectrometry Identification of Yeast Species in Blood Cultures." Journal of Clinical Microbiology 53, no. 5 (2015): 1761–64. http://dx.doi.org/10.1128/jcm.03600-14.

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24

Martiny, D., A. Dediste, and O. Vandenberg. "Comparison of an in-house method and the commercial Sepsityper™ kit for bacterial identification directly from positive blood culture broths by matrix-assisted laser desorption-ionisation time-of-flight mass spectrometry." European Journal of Clinical Microbiology & Infectious Diseases 31, no. 9 (2012): 2269–81. http://dx.doi.org/10.1007/s10096-012-1566-1.

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25

Schieffer, K. M., K. E. Tan, P. D. Stamper, et al. "Multicenter evaluation of the Sepsityper™ extraction kit and MALDI-TOF MS for direct identification of positive blood culture isolates using the BD BACTEC™ FX and VersaTREK® diagnostic blood culture systems." Journal of Applied Microbiology 116, no. 4 (2014): 934–41. http://dx.doi.org/10.1111/jam.12434.

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26

Ponderand, Léa, Patricia Pavese, Danièle Maubon, et al. "Evaluation of Rapid Sepsityper® protocol and specific MBT-Sepsityper module (Bruker Daltonics) for the rapid diagnosis of bacteremia and fungemia by MALDI-TOF-MS." Annals of Clinical Microbiology and Antimicrobials 19, no. 1 (2020). http://dx.doi.org/10.1186/s12941-020-00403-w.

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AbstractDuring bloodstream infections, rapid adaptation of empirical treatment according to the microorganism identified is essential to decrease mortality. The aim of the present study was to assess the microbiological performances of a new rapid version of the Sepsityper® kit (Bruker Daltonics) allowing identification of bacteria and yeast by MALDI-TOF mass spectrometry directly from positive blood cultures in 10 min and of the specific MBT-Sepsityper module for spectra analysis, designed to increase identification performance. Identification rates were determined prospectively on 350 bacter
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27

Kittel, Maximilian, Anna Greulich, Peter Findeisen, et al. "Direct MS-Based Bacterial Identification of Blood Cultures. Comparison of an In-House TritonX-Protocol with Bruker-Sepsityper™." Clinical Laboratory 64, no. 11+12/2018 (2018). http://dx.doi.org/10.7754/clin.lab.2018.180522.

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28

Bulane, Atang, and Anwar Hoosen. "Use of matrix-assisted laser desorption/ionisation-time of flight mass spectrometry analyser in a diagnostic microbiology laboratory in a developing country." African Journal of Laboratory Medicine 6, no. 1 (2017). http://dx.doi.org/10.4102/ajlm.v6i1.598.

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Background: Rapid and accurate identification of pathogens is of utmost importance for management of patients. Current identification relies on conventional phenotypic methods which are time consuming. Matrix-assisted laser desorption/ionisation-time of flight mass spectrometry (MALDI-TOF MS) is based on proteomic profiling and allows for rapid identification of pathogens.Objective: We compared MALDI-TOF MS against two commercial systems, MicroScan Walkaway and VITEK 2 MS.Methods: Over a three-month period from July 2013 to September 2013, a total of 227 bacteria and yeasts were collected from
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