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Artículos de revistas sobre el tema "Wall label"

Autor: Grafiati

Publicado: 7 de diciembre de 2024

Última modificación: 31 de julio de 2025

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1

Sonnenfeld, E. M., T. J. Beveridge, and R. J. Doyle. "Discontinuity of charge on cell wall poles of Bacillus subtilis." Canadian Journal of Microbiology 31, no. 9 (1985): 875–77. http://dx.doi.org/10.1139/m85-163.

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When cell wall poles of Bacillus subtilis were treated with dilute cationized ferritin, label was found only at discrete patches. Since cationized ferritin binds to negatively charged groups, the pole regions that retain label most likely represent localized surface sites of high electronegativity, indicating that the cell wall of B. subtilis is, at least, partially differentiated.

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2

Di Tommaso, Stefania, Sherrie Wang, and David B. Lobell. "Combining GEDI and Sentinel-2 for wall-to-wall mapping of tall and short crops." Environmental Research Letters 16, no. 12 (2021): 125002. http://dx.doi.org/10.1088/1748-9326/ac358c.

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Abstract High resolution crop type maps are an important tool for improving food security, and remote sensing is increasingly used to create such maps in regions that possess ground truth labels for model training. However, these labels are absent in many regions, and models trained on optical satellite features often exhibit low performance when transferred across geographies. Here we explore the use of NASA’s global ecosystem dynamics investigation (GEDI) spaceborne lidar instrument, combined with Sentinel-2 optical data, for crop type mapping. Using data from three major cropped regions (in

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3

Rogers, Gregory S., and John J. Frett. "ORGANELLE-SPECIFIC LOCALIZATION OF IPTASE IN TRANSGENIC NICOTIANA." HortScience 31, no. 6 (1996): 912C—912. http://dx.doi.org/10.21273/hortsci.31.6.912c.

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Nicotiana transformed with the isopentenyl transferase (ipt) gene from Agrobacterium tumefaciens was fixed for 1 h in 1% glutaraldehyde and 4% formaldehyde. Ultrathin sections were collected on nickel grids. Grids were treated with polyclonal anti-IPTase antibody raised in rabbits and visualized with 10 nm, protein-A–labeled colloidal gold. Gold label was found throughout the cell, including the cell wall, vacuole, rough ER, and organelles. Cell wall and vacuole labeling appears to be due to non-specific binding and is greatly reduced by a BSA block. Mitochondria and chloroplasts also showed g

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4

Messner, Robert, and Christian P. Kubicek. "Synthesis of cell wall glucan, chitin, and protein by regenerating protoplasts and mycelia of Trichoderma reesei." Canadian Journal of Microbiology 36, no. 3 (1990): 211–17. http://dx.doi.org/10.1139/m90-036.

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The synthesis of constituent polymers of the cell wall by either growing mycelia or regenerating protoplasts of Trichoderma reesei QM 9414 was investigated by following the incorporation of radioactive precursors of the individual polymers (i.e., N[14C] acetyl-glucosamine, [3H] and [14C]glucose, [14C]mannose, and [35S]methionine) into individual fractions. N-Acetyl-glucosamine and glucose were found to become specifically incorporated into cell wall chitin and glucan by both mycelia and regenerating protoplasts, indicating the activity of chitin and glucan synthases in both systems. Cell wall

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5

Di Guilmi, A. M., J. Bonnet, S. Peiβert, et al. "Specific and spatial labeling of choline-containing teichoic acids in Streptococcus pneumoniae by click chemistry." Chemical Communications 53, no. 76 (2017): 10572–75. http://dx.doi.org/10.1039/c7cc05646j.

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6

Koh, Jia Xin, Keyu Geng, and Donglin Jiang. "Smart covalent organic frameworks: dual channel sensors for acids and bases." Chemical Communications 57, no. 74 (2021): 9418–21. http://dx.doi.org/10.1039/d1cc03057d.

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7

Beise, Frank, Harald Labischinski, and Hans Bradaczek. "On the Relationships between Molecular Conformation, Affinity towards Penicillin-Binding Proteins, and Biological Activity of Penicillin G-Sulfoxide." Zeitschrift für Naturforschung C 43, no. 9-10 (1988): 656–64. http://dx.doi.org/10.1515/znc-1988-9-1006.

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Abstract The binding capacity of penicillin G-sulfoxide towards the penicillin-binding proteins (PBP) of Staphylococcus aureus H was studied. The sulfoxide and its parent compound, penicillin G , differ only in two aspects, the sulfur-bound oxygen and an altered conformation of the five-membered thiazolidine-ring system. These minor alterations of the penicillin structure resulted in a drastical decrease of binding activity (about two orders of magnitude) of the sulfoxide derivative towards its target enzymes. Furthermore, the sulfoxide did not exhibit the selectivity of subinhibitory doses fo

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8

Mackey, Brandon, and Vernon T. Mackey. "Successful Deoxycholate Acid Use in a Male Patient for Resistant Lower Abdominal Wall Fat." SKIN The Journal of Cutaneous Medicine 4, no. 4 (2020): 349–52. http://dx.doi.org/10.25251/skin.4.4.12.

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In this patient off-label Kybella was effective in improving the contour and removing excess lower abdominal fat after two treatments. While off-label use of Kybella is commonly used every day by physicians across the United States (8, 10), it is not well represented in the literature (9). This case is presented to aid in the discussion of new, safe, noninvasive treatment techniques in which healthcare demand and delivery has outpaced publication and literature support.

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9

Gu, Hongjun, He Gong, Chunxue Wang, et al. "Compact Inner-Wall Grating Slot Microring Resonator for Label-Free Sensing." Sensors 19, no. 22 (2019): 5038. http://dx.doi.org/10.3390/s19225038.

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In this paper, we present and analyze a compact inner-wall grating slot microring resonator (IG-SMRR) with the footprint of less than 13 μm × 13 μm on the silicon-on-insulator (SOI) platform for label-free sensing, which comprises a slot microring resonator (SMRR) and inner-wall grating (IG). Its detection range is significantly enhanced without the limitation of the free spectral region (FSR) owing to the combination of SMRR and IG. The IG-SMRR has an ultra-large quasi-FSR of 84.5 nm as the detection range, and enlarged factor is up to over 3 compared with the conventional SMRR. The concentra

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10

Zhang, Yaowen, Linsheng Huo, and Hongnan Li. "Automated Recognition of a Wall between Windows from a Single Image." Journal of Sensors 2017 (2017): 1–8. http://dx.doi.org/10.1155/2017/7051931.

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To avoid the time-consuming, costly, and expert-dependent traditional assessment of earthquake damaged structures, image-based automatic methods have been developed recently. Since automated recognition of structure elements is the basis by which these methods achieve automatic detection, this study proposes a method to recognize the wall between windows from a single image automatically. It begins from detection of line segments with further selection and linking to obtain longer line segments. The color features of the two sides of each long line segment are employed to pick out line segment

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11

Ouellette, Guillemond B., Mohamed Cherif, Marie Simard, and Louis Bernier. "Histopathology of Fusarium wilt of staghorn sumac (Rhus typhina) caused by Fusarium oxysporum f. sp. callistephi race 3. I. Modes of tissue colonization and pathogen peculiarities." Phytoprotection 86, no. 3 (2006): 157–74. http://dx.doi.org/10.7202/013074ar.

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Abstract Light and transmission electron microscope studies of naturally infected or inoculated staghorn sumac plants by Fusarium oxysporum f. sp. callistephi race 3 are reported. Diverse extrinsic material (including latex in some instances) or elements occurred in vessel lumina. Some of this material labelled for pectin, often in association with tyloses, as did other opaque matter in paratracheal cells, related to alterations of their protective layer. Pronounced alterations of pit membranes of bordered pits occurred, with their outer portions disrupted into bodies of opaque matter, strongl

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12

Hazan, L., G. Focht, N. Gavrielov, et al. "P269 Harnessing Natural Language Processing for Structured Information Extraction from Radiology Reports in Crohn's Disease: A Nationwide Study From the epi-IIRN." Journal of Crohn's and Colitis 18, Supplement_1 (2024): i633—i634. http://dx.doi.org/10.1093/ecco-jcc/jjad212.0399.

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Abstract Background Large-scale analyses of imaging in Crohn's disease (CD) hold promise for advancing research on the inflammatory burden in the bowel as well as developing predictive models of disease progression. However, the lack of structured human annotations in these datasets limits the ability use these for research. This study from epi-IIRN nationwide cohort, aims to develop and evaluate Natural Language Processing (NLP) algorithms for extracting structured information from unstructured radiology reports on CT and MR enterography. Methods We identified radiology reports of all patient

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13

HUGHES, D., S. TALWAR, I. B. SQUIRE, J. E. DAVIES, and L. L. NG. "An immunoluminometric assay for N-terminal pro-brain natriuretic peptide: development of a test for left ventricular dysfunction." Clinical Science 96, no. 4 (1999): 373–80. http://dx.doi.org/10.1042/cs0960373.

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Measurement of plasma levels of brain natriuretic peptide (BNP) has been used to assess left ventricular dysfunction and prognosis. Levels of the N-terminus of the precursor of BNP (NT-proBNP) have been reported to be elevated to a greater extent than BNP in left ventricular dysfunction. We have devised a non-radioactive sensitive and specific assay for NT-proBNP based on a competitive ligand binding principle. The chemiluminescent label 4-(2-succinimidyloxycarbonylethyl)phenyl-10-methylacridinium 9-carboxylate fluorosulphonate was used to label peptides representing domains in the middle and

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14

Domozych, David S., Hannah Brechka, Alicia Britton, and Marc Toso. "Cell Wall Growth and Modulation Dynamics in a Model Unicellular Green Alga—Penium margaritaceum: Live Cell Labeling with Monoclonal Antibodies." Journal of Botany 2011 (February 16, 2011): 1–8. http://dx.doi.org/10.1155/2011/632165.

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Penium margaritaceum is a unicellular charophycean green alga that possesses cell wall polymers similar to those of land plants. Several wall macromolecules of this alga are recognized by monoclonal antibodies specific for wall polymer epitopes of land plants. Immunofluorescence protocols using these antibodies may be employed to label specific cell wall constituents of live cells. Fluorescent labeling persists for several days, and this attribute allows for tracing of wall epitopes in both long- and short-term studies of cell development. Quantitative analysis of surface area covered by cell

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15

Ouellette, G. B., R. P. Baayen, H. Chamberland, M. Simard, D. Rioux, and P. M. Charest. "Cytochemical Labeling for Fungal and Host Components in Plant Tissues Inoculated with Fungal Wilt Pathogens." Microscopy and Microanalysis 10, no. 4 (2004): 449–61. http://dx.doi.org/10.1017/s1431927604040796.

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Antibodies to detect pectin in present investigations attached to distinct fibrils in vessel lumina. In carnation infected with an isolate ofFusarium oxysporumf.sp., labeling of pathogen cells also occurred; in a resistant cultivar (cv.), it was coincident with proximate pectin fibrils and linked to altered fungal walls, which was the opposite in the susceptible cv., indicating that hindrance of pathogen ability to degrade pectin may be related to resistance. Labeling of the fungus in culture was nil, except in media containing pectin, showing that pectin is not native to the pathogen. Labelin

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16

Rae, Angus E., Xiaoyang Wei, Neftali Flores-Rodriguez, David W. McCurdy, and David A. Collings. "Super-Resolution Fluorescence Imaging of Arabidopsis thaliana Transfer Cell Wall Ingrowths using Pseudo-Schiff Labelling Adapted for the Use of Different Dyes." Plant and Cell Physiology 61, no. 10 (2020): 1775–87. http://dx.doi.org/10.1093/pcp/pcaa102.

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Abstract To understand plant growth and development, it is often necessary to investigate the organization of plant cells and plant cell walls. Plant cell walls are often fluorescently labeled for confocal imaging with the dye propidium iodide using a pseudo-Schiff reaction. This reaction binds free amine groups on dye molecules to aldehyde groups on cellulose that result from oxidation with periodic acid. We tested a range of fluorescent dyes carrying free amine groups for their ability to act as pseudo-Schiff reagents. Using the low-pH solution historically used for the Schiff reaction, thes

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17

Huang, Weinan, Yating Nie, Nan Zhu, et al. "Hybrid Label-Free Molecular Microscopies for Simultaneous Visualization of Changes in Cell Wall Polysaccharides of Peach at Single- and Multiple-Cell Levels during Postharvest Storage." Cells 9, no. 3 (2020): 761. http://dx.doi.org/10.3390/cells9030761.

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Softening of fruit during the postharvest storage, which is mainly associated with both compositional and spatial changes of polysaccharides within cell wall, affects the texture and quality of fruit. Current research on the fruit softening mechanism lacks an understanding of the overall softening at the cell level. The objective of this work was to investigate the change in the spatial distribution of cell wall polysaccharides in peach flesh cells at both single- and multiple-cell levels in a label-free way during the postharvest storage. Nonmelting peaches (Prunus persica L. Batsch cv.”Zhong

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18

Keithley, Elizabeth M., Steve Horowitz, and Michael J. Ruckenstein. "NA,K-Atpase in the Cochlear Lateral Wall of Human Temporal Bones with Endolymphatic Hydrops." Annals of Otology, Rhinology & Laryngology 104, no. 11 (1995): 858–63. http://dx.doi.org/10.1177/000348949510401106.

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Meniere's disease has traditionally been thought to arise from a disruption in longitudinal endolymphatic flow. This view has been brought into question by recent experimental studies that have focused attention on derangements of cochlear fluid and electrolyte homeostatic mechanisms in Meniere's disease, including abnormalities in Na,K-ATPase enzymes found in the cochlear lateral wall. The current study examined the immunohistochemical labeling pattern of the major ion-transporting enzyme of the stria vascularis, Na,K-ATPase, in archival sections of hydropic and nonhydropic human temporal bon

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19

Lin, Junyu, Jun Hu, Zhiyuan Xie, Yulan Zhang, Guangjia Huang, and Zengping Chen. "A Multitask Network for People Counting, Motion Recognition, and Localization Using Through-Wall Radar." Sensors 23, no. 19 (2023): 8147. http://dx.doi.org/10.3390/s23198147.

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Due to the outstanding penetrating detection performance of low-frequency electromagnetic waves, through-wall radar (TWR) has gained widespread applications in various fields, including public safety, counterterrorism operations, and disaster rescue. TWR is required to accomplish various tasks, such as people detection, people counting, and positioning in practical applications. However, most current research primarily focuses on one or two tasks. In this paper, we propose a multitask network that can simultaneously realize people counting, action recognition, and localization. We take the ran

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20

Do, Truc, Julia E. Page, and Suzanne Walker. "Uncovering the activities, biological roles, and regulation of bacterial cell wall hydrolases and tailoring enzymes." Journal of Biological Chemistry 295, no. 10 (2020): 3347–61. http://dx.doi.org/10.1074/jbc.rev119.010155.

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Bacteria account for 1000-fold more biomass than humans. They vary widely in shape and size. The morphological diversity of bacteria is due largely to the different peptidoglycan-based cell wall structures that encase bacterial cells. Although the basic structure of peptidoglycan is highly conserved, consisting of long glycan strands that are cross-linked by short peptide chains, the mature cell wall is chemically diverse. Peptidoglycan hydrolases and cell wall–tailoring enzymes that regulate glycan strand length, the degree of cross-linking, and the addition of other modifications to peptidog

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21

Horne, Diane, and Alexander Tomasz. "Factors affecting sensitivity of group B streptococci to an exogenous murein hydrolase." Canadian Journal of Microbiology 31, no. 5 (1985): 417–22. http://dx.doi.org/10.1139/m85-078.

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Group B streptococci treated with cell wall synthesis inhibitors (penicillin or vancomycin) or by a variety of membrane-acting agents are sensitized to the lytic action of exogenous M1 muramidase. Muramidase without a sensitizing agent caused rupture of bacterial chains only, accompanied by the release of a small amount of cell wall peptidoglycan label and an increase of the number of colony-forming units. In combination with sensitizing agents the exogenous muramidase appeared to initiate hydrolysis of biosynthetically new peptidoglycan. Treatment of the cells with chloramphenicol or starvati

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22

Ouellette, G. B., R. P. Baayen, M. Simard, and D. Rioux. "Ultrastructural and cytochemical study of colonization of xylem vessel elements of susceptible and resistant Dianthus caryophyllus by Fusarium oxysporum f.sp. dianthi." Canadian Journal of Botany 77, no. 5 (1999): 644–63. http://dx.doi.org/10.1139/b99-033.

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The colonization processes of the xylem in the susceptible carnation cv. Early Sam and the resistant cv. Novada were studied ultrastructurally following inoculation with Fusarium oxysporum f.sp. dianthi. Samples from 1 to 3 cm above the incision were collected over 5 weeks and processed following conventional procedures as well as with probes for cellulose, N-acetyl-glucosamine, and pectin. The fungus grew profusely in the vessel lumina of the susceptible cultivar. Some of the colonized vessels were lined with coating material connected to the fungal cell wall and extending into the host cell

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23

MONTES, J. F., M. DURFORT, A. LLADÓ, and J. GARCÍA-VALERO. "Characterization and immunolocalization of a main proteinaceous component of the cell wall of the protozoan parasite Perkinsus atlanticus." Parasitology 124, no. 5 (2002): 477–84. http://dx.doi.org/10.1017/s0031182002001415.

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Described in the present study is a major component of the cell wall of 2 of the most pathogenic parasites of molluscs, Perkinsus atlanticus and P. marinus. The component is a high molecular weight protein (233 kDa), which we have named PWP-1 (for Perkinsus wall protein-1). Western blots, using a polyclonal serum generated against purified PWP-1 from P. atlanticus, revealed that this protein is expressed by all walled developmental stages of this protozoon. By means of immunogold electron microscopy, labelling for PWP-1 was strong and specifically associated with the cell wall. The label densi

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24

Massicotte, H. B., L. H. Melville, L. E. Tackaberry, and R. L. Peterson. "A comparative study of mycorrhizas in several genera of Pyroleae (Ericaceae) from western Canada." Botany 86, no. 6 (2008): 610–22. http://dx.doi.org/10.1139/b08-027.

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Genera in the tribe Pyroleae (subfamily Monotropoideae, family Ericaceae) occur as understory plants in northern temperate zones where some form major components of ecosystems. Most have been poorly studied in terms of their association with symbiotic fungi. In this study, colonization patterns of mycorrhizal roots of five members of the Pyroleae ( Pyrola asarifolia Michx., Pyrola chlorantha Sw., Orthilia secunda (L.) House, Chimaphila umbellata (L.) W. Bart., Moneses uniflora (L.) Gray) were explored. Root samples were processed for light, fluorescence, and laser scanning confocal, scanning e

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25

Ouellette, G. B., H. Chamberland, A. Goulet, M. Lachapelle, and J. G. Lafontaine. "Fine structure of the extracellular sheath and cell walls inOphiostoma novo-ulmigrowing on various substrates." Canadian Journal of Microbiology 45, no. 7 (1999): 582–97. http://dx.doi.org/10.1139/w99-045.

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The presence of microfilamentous-like structures of tubular appearance (MFS) in cell walls and extracellular sheath material (ES) in a number of isolates of Ophiostoma novo-ulmi Brasier grown on various substrates and following various treatments is reported. Standard fixation or high-pressure freezing methods were used, and cytochemical tests were carried out to detect fungal and host wall components and, in some cases, fungal DNA. In some cases, serial 0.2-μm-thick sections were examined at 120 kV and tilted to obtain stereoscopic images. Whether the fungal cell walls were thick and composed

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26

Morel, Oriane, Cedric Lion, Godfrey Neutelings, et al. "REPRISAL: mapping lignification dynamics using chemistry, data segmentation, and ratiometric analysis." Plant Physiology 188, no. 2 (2021): 816–30. http://dx.doi.org/10.1093/plphys/kiab490.

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Abstract This article describes a methodology for detailed mapping of the lignification capacity of plant cell walls that we have called “REPRISAL” for REPorter Ratiometrics Integrating Segmentation for Analyzing Lignification. REPRISAL consists of the combination of three separate approaches. In the first approach, H*, G*, and S* monolignol chemical reporters, corresponding to p-coumaryl alcohol, coniferyl alcohol, and sinapyl alcohol, are used to label the growing lignin polymer in a fluorescent triple labeling strategy based on the sequential use of three main bioorthogonal chemical reactio

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27

Bayman, Neil, Wiebke Appel, Linda Ashcroft, et al. "Prophylactic Irradiation of Tracts in Patients With Malignant Pleural Mesothelioma: An Open-Label, Multicenter, Phase III Randomized Trial." Journal of Clinical Oncology 37, no. 14 (2019): 1200–1208. http://dx.doi.org/10.1200/jco.18.01678.

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PURPOSE Prophylactic irradiation to the chest wall after diagnostic or therapeutic procedures in patients with malignant pleural mesothelioma (MPM) has been a widespread practice across Europe, although the efficacy of this treatment is uncertain. In this study, we aimed to determine the efficacy of prophylactic radiotherapy in reducing the incidence of chest wall metastases (CWM) after a procedure in MPM. METHODS After undergoing a chest wall procedure, patients with MPM were randomly assigned to receive prophylactic radiotherapy (within 42 days of the procedure) or no radiotherapy. Open thor

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28

Andito, Tegar, Agni Saraswati, and Elatulada Catur Tama. "Penyuluhan Seni Branding dan Identitas Kelompok Seni “Adhikari Creations”." Jurnal Pengabdian Seni 1, no. 2 (2020): 84–94. http://dx.doi.org/10.24821/jas.v1i2.4712.

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Adhikari Creations adalah sebuah UMKM kerajinan tangan yang memproduksi produk-produk sablon manual dengan diterapkan pada bantal, wall art, planter, dan bucket dengan visual yang umumnya berupa decorative quotes. Segmentasi pasar dari produk-produk Adhikari Creations adalah penggemar decorative quotes di mana umumnya adalah masyarakat menengah ke atas. Selain melayani pesanan perorangan, sejumlah pelanggan korporat juga menjadi pelanggan tetap dari Adhikari Creaton. Dalam mempromosikan produknya, Adhikari Creations memanfaatkan sosial media Instagram sebagai media utamanya. Walaupun sudah dik

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Cheraghi, Somaye, Mohammad A. Taher, Hassan Karimi-Maleh, and Ehsan Faghih-Mirzaei. "A nanostructure label-free DNA biosensor for ciprofloxacin analysis as a chemotherapeutic agent: an experimental and theoretical investigation." New Journal of Chemistry 41, no. 12 (2017): 4985–89. http://dx.doi.org/10.1039/c7nj00609h.

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A nanostructure DNA biosensor based on pencil graphite electrode modified with polypyrrole, single wall carbon nanotubes and ds-DNA (PGE/PP/SWCNTs/DNA) was suggested for the determination of ciprofloxacin.

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Elia, M., N. J. Fuller, and P. R. Murgatroyd. "Measurement of bicarbonate turnover in humans: applicability to estimation of energy expenditure." American Journal of Physiology-Endocrinology and Metabolism 263, no. 4 (1992): E676—E687. http://dx.doi.org/10.1152/ajpendo.1992.263.4.e676.

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Bicarbonate turnover and energy expenditure were assessed in six healthy male volunteers, by the use of a constant infusion of radiolabeled bicarbonate (NaH14CO3) administered over 36 h, while the volunteers were confined to a whole body indirect calorimeter. Recovery and dilution of isotope were assessed from measurements made on continuous collections of CO2, entering and leaving the calorimeter, urine, and intermittent spot breath and saliva samples. Mean recovery of infused label in gaseous CO2 was 95.6 +/- 1.1% (SD) between 12 and 36 h. Applying a 95% mean recovery of label to each subjec

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31

Manocha, M. S., and C. M. McCullough. "Suppression of host cell wall synthesis at penetration sites in a compatible interaction with a mycoparasite." Canadian Journal of Botany 63, no. 5 (1985): 967–73. http://dx.doi.org/10.1139/b85-130.

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Differences in cell wall reactions at the penetration sites on a resistant host, Phascolomyces articulosus Boedijn ex Benny and Benjamin, and on a susceptible host, Choanephora cucurbitarum (Berk. & Rav.) Thaxter, were studied by autoradiography at 18 h after inoculation with a mycoparasite, Piptocephalis virginiana Leadbeater and Mercer. Localized incorporation of a radioactive chitin precursor, N-[3H]acetylglucosamine ([3H]GlcNAc), was observed at the penetration sites on the resistant but not the susceptible host. Autoradiography of mechanically wounded hyphae of the susceptible and res

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32

Morioka, H., A. Suganuma, and M. Tachibana. "Localization of sugar-binding sites in Staphylococcus aureus using gold-labeled neoglycoprotein." Journal of Histochemistry & Cytochemistry 42, no. 12 (1994): 1609–13. http://dx.doi.org/10.1177/42.12.7983361.

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We studied post- and pre-embedding staining of sugar-binding sites on thin sections of Staphylococcus aureus with an electron microscopic neoglycoprotein-gold technique. Although gold particles of cellobiosyl bovine serum albumin (BSA)-glycosylated BSA-, lactosyl BSA-, and melibiosyl BSA-gold did not label, heavy labeling of N-acetylglucosaminide-BSA-gold was observed in both the cell wall and the cytoplasm on Spurr-embedded thin sections of S. aureus. Inhibition of labeling with wheat germ agglutinin-biotin and N-acetylglucosaminidase indicated that the labeling was due to N-acetylglucosamine

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33

De Ruvo, Ermenegildo, Lucia De Luca, Domenico Grieco, et al. "Tissue thickness measured with dielectric-based technology during radiofrequency catheter ablation." European Heart Journal Supplements 25, Supplement_C (2023): C253—C257. http://dx.doi.org/10.1093/eurheartjsupp/suad049.

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Abstract Radiofrequency catheter ablation of the cavotricuspid isthmus is the standard treatment for patients suffering from typical atrial flutter. The aim of this study was to test the feasibility of tissue thickness and lesion transmurality measurement by a novel dielectric system. This was a retrospective multicentric non-randomized open-label, single-arm study. The atrial wall thickness was significantly higher close to the tricuspid annulus than close to the inferior vena cava and a trend towards a progressive decrease of atrial wall thickness was observed moving the mapping catheter fro

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Cheng, Qi, Yongzheng Zhu, Kaifei Deng, et al. "Label-Free Diagnosis of Pulmonary Fat Embolism Using Fourier Transform Infrared (FT-IR) Spectroscopic Imaging." Applied Spectroscopy 76, no. 3 (2022): 352–60. http://dx.doi.org/10.1177/00037028211061430.

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The diagnosis of pulmonary fat embolism (PFE) is of great significance in the field of forensic medicine because it can be considered a major cause of death or a vital reaction. Conventional histological analysis of lung tissue specimens is a widely used method for PFE diagnosis. However, variable and labor-intensive tissue staining procedures impede the validity and informativeness of histological image analysis. To obtain complete information from tissues, a method based on infrared imaging of unlabeled tissue sections was developed to identify pulmonary fat emboli in the present study. We s

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Rusu, Mihai Stelian, Corneliu Pintilescu, and Dalia Báthory. "“Mister Gorbachev, tear down this wall!” Socio‑economic and Political Consequences 30 Years After." History of Communism in Europe 10 (2019): 7–17. http://dx.doi.org/10.5840/hce2019101.

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The fall of the Berlin Wall stood for a symbol of change and freedom across the socialist bloc and inspired the inhabitants in Eastern Europe to take action and revolt against dictatorial regimes. A long and often painful process of social, economic and political transformation began. Scholars grouped their research dealing with such transformations under the label of “Transitology” and the developing subfields of “transitional justice” and “memory studies” expanded and caught the academic interest. The present argument looks at the emergence and evolvement of these fields in parallel with a g

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Sikora, Michal, David Scheiner, Cornelia Betschart, et al. "Label-free, three-dimensional multiphoton microscopy of the connective tissue in the anterior vaginal wall." International Urogynecology Journal 26, no. 5 (2014): 685–91. http://dx.doi.org/10.1007/s00192-014-2571-y.

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37

Reis, Sofia F., Pedro A. R. Fernandes, Vítor J. Martins, et al. "Brewer’s Spent Yeast Cell Wall Polysaccharides as Vegan and Clean Label Additives for Mayonnaise Formulation." Molecules 28, no. 8 (2023): 3540. http://dx.doi.org/10.3390/molecules28083540.

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Brewer’s spent yeast (BSY) mannoproteins have been reported to possess thickening and emulsifying properties. The commercial interest in yeast mannoproteins might be boosted considering the consolidation of their properties supported by structure/function relationships. This work aimed to attest the use of extracted BSY mannoproteins as a clean label and vegan source of ingredients for the replacement of food additives and protein from animal sources. To achieve this, structure/function relationships were performed by isolating polysaccharides with distinct structural features from BSY, either

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38

Heywood, Wendy E., Jon Searle, Richard Collis, et al. "A Proof of Principle 2D Spatial Proteome Mapping Analysis Reveals Distinct Regional Differences in the Cardiac Proteome." Life 14, no. 8 (2024): 970. http://dx.doi.org/10.3390/life14080970.

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Proteomics studies often explore phenotypic differences between whole organs and systems. Within the heart, more subtle variation exists. To date, differences in the underlying proteome are only described between whole cardiac chambers. This study, using the bovine heart as a model, investigates inter-regional differences and assesses the feasibility of measuring detailed, cross-tissue variance in the cardiac proteome. Using a bovine heart, we created a two-dimensional section through a plane going through two chambers. This plane was further sectioned into 4 × 4 mm cubes and analysed using la

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39

Bourett, Timothy M., and Richard J. Howard. "In vitro development of penetration structures in the rice blast fungus Magnaporthe grisea." Canadian Journal of Botany 68, no. 2 (1990): 329–42. http://dx.doi.org/10.1139/b90-044.

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The development of appressoria, penetration pegs, and infection hyphalike structures of Magnaporthe grisea on a cellophane substratum were studied using light and electron microscopy of freeze-substituted specimens. Developing, hyaline appressoria contained numerous membrane cisternae and cytoplasmic vesicles. These vesicles were concentrated near the substratum where the cell wall was very thin and then absent. Vesicles and cisternae were not present in appressoria during melanization. The discrete melanin layer contained a heretofore undescribed microfibrillar component that did not label wi

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40

Huysamer, Marius, L. Carl Greve, and John M. Labavitch. "CELL WALL COMPOSITION AND SYNTHETIC CAPACITY OF THE PERICARP OF RIPENING `JACKPOT' TOMATO." HortScience 27, no. 6 (1992): 651c—651. http://dx.doi.org/10.21273/hortsci.27.6.651c.

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Discs from outer pericarp of mature green (MG) and light red (LR) tomatoes were incubated with 13C6-glucose as precursor to cell wall constituents, to determine biosynthetic capacity of the outer 2mm (including cuticle) and adjacent inner 2mm of tissue. Cell wall material was fractionated into pectic and hemicellulosic classes by sequential extraction, and alditol acetates and partially-methylated alditol acetates were prepared. Neutral sugars (NS), glycosidic linkage compositions and incorporation of label were determined by GC-FID and GC-MS. Rhamnose, arabinose and galactose accounted for ca

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41

Hashimoto, Sin-ichi, Nai-Hsuan Chen, Yuji Suzuki, and Nobuhide Kasagi. "MNM-P9-3 Label-Free Continuous Micro Cell Sorting Using Specific Wall Adhesion And Secondary Flow." Proceedings of the Symposium on Micro-Nano Science and Technology 2010.2 (2010): 127–28. http://dx.doi.org/10.1299/jsmemnm.2010.2.127.

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42

Feng, Hongping, Waiwai Mon, Xiaoxia Su, et al. "Integrated Biological Experiments and Proteomic Analyses of Nicotiana tabacum Xylem Sap Revealed the Host Response to Tomato Spotted Wilt Orthotospovirus Infection." International Journal of Molecular Sciences 25, no. 20 (2024): 10907. http://dx.doi.org/10.3390/ijms252010907.

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The plant vascular system is not only a transportation system for delivering nutrients but also a highway transport network for spreading viruses. Tomato spotted wilt orthotospovirus (TSWV) is among the most destructive viruses that cause serious losses in economically important crops worldwide. However, there is minimal information about the long-distance movements of TSWV in the host plant vascular system. In this this study, we confirm that TSWV virions are present in the xylem as observed by transmission electron microscopy (TEM). Further, a quantitative proteomic analysis based on label-f

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43

Zhang, Hanxiao, Albert Zehan Li, and Juewen Liu. "Surfactant-Assisted Label-Free Fluorescent Aptamer Biosensors and Binding Assays." Biosensors 13, no. 4 (2023): 434. http://dx.doi.org/10.3390/bios13040434.

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Using DNA staining dyes such as SYBR Green I (SGI) and thioflavin T (ThT) to perform label-free detection of aptamer binding has been performed for a long time for both binding assays and biosensor development. Since these dyes are cationic, they can also adsorb to the wall of reaction vessels leading to unstable signals and even false interpretations of the results. In this work, the stability of the signal was first evaluated using ThT and the classic adenosine aptamer. In a polystyrene microplate, a drop in fluorescence was observed even when non-binding targets or water were added, whereas

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44

Rogers, Gregory S., and John J. Frett. "PRELIMINARY LOCALIZATION OF IPTASE IN TRANSGENIC NICOTIANA." HortScience 30, no. 2 (1995): 187a—187. http://dx.doi.org/10.21273/hortsci.30.2.187a.

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Nicotiana transformed with the isopentenyl transferase (ipt) gene from Agrobacterium tumefaciens was fixed in 1% gluterdehyde and 4% formaldehyde for 1 h. Grids were treated with polygonal anti-IpTase antibody raised in rabbits and visualized with 10 nm protein-A-labeled colloidal gold. Initial localization was performed on Nicotiana transformed with the ipt gene under the control of the 35S promoter from cauliflower mosaic virus. Colloidal gold was found throughout the cell, including the cell wall, vacuole, and rough ER. Cell wall and vacuole labeling appears to be due to nonspecific binding

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45

Liu, Fei, Guiming Xiang, Liqun Zhang, et al. "A novel label free long non-coding RNA electrochemical biosensor based on green l-cysteine electrodeposition and Au–Rh hollow nanospheres as tags." RSC Advances 5, no. 64 (2015): 51990–99. http://dx.doi.org/10.1039/c5ra07904g.

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lncRNA biosensor based on single-wall carbon nanotubes wrapped with Au–Rh hollow nanospheres (Au/Rh-HNP@SWCNT) complex signal amplification and an l-Cys Au nano-film provided ultrasensitive detection for the nuclear paraspeckle assembly transcript 1 (NEAT1).

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46

Xie, Liping, Xiaojun Yan, and Yanan Du. "An aptamer based wall-less LSPR array chip for label-free and high throughput detection of biomolecules." Biosensors and Bioelectronics 53 (March 2014): 58–64. http://dx.doi.org/10.1016/j.bios.2013.09.031.

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47

Tam, Phuong Dinh, Nguyen Van Hieu, Nguyen Duc Chien, Anh-Tuan Le, and Mai Anh Tuan. "DNA sensor development based on multi-wall carbon nanotubes for label-free influenza virus (type A) detection." Journal of Immunological Methods 350, no. 1-2 (2009): 118–24. http://dx.doi.org/10.1016/j.jim.2009.08.002.

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48

Schmidt, M., A. M. Schwartzberg, P. N. Perera, et al. "Label-free in situ imaging of lignification in the cell wall of low lignin transgenic Populus trichocarpa." Planta 230, no. 3 (2009): 589–97. http://dx.doi.org/10.1007/s00425-009-0963-x.

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Hăisan, Daniela. "(STEREO)TYPICAL ISSUES IN TEACHING AND LEARNING NOUNS AND NOUN PHRASES." Messages, Sages and Ages 6, 2019, no. 2 (2019): 17–20. https://doi.org/10.5281/zenodo.3556504.

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Starting from our own experience of teaching morphology (the noun phrase) to first-year students, we are going to identify and label the main challenges this grammar issue poses to both English majors and minors. These challenges have to do basically with “fuzzy concepts” and the interrelation of syntax and semantics.<em> </em>Our beyond-methods approach, based on empirical observation(s) and assumptions as a result of closely monitoring and evaluating the learning process, heavily relies on the “organic metaphor” according to which second language acquisition is more l

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50

Pendland, J. C., and D. G. Boucias. "Ultrastructural localization of carbohydrate in cell walls of the entomogenous hyphomycete Nomuraea rileyi." Canadian Journal of Microbiology 38, no. 5 (1992): 377–86. http://dx.doi.org/10.1139/m92-064.

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Several probes were used in this ultrastructural study to localize polysaccharides in cell walls on conidial germ tubes, hyphal bodies, and mycelia of the entomogenous hyphomycete Nomuraea rileyi. With the exception of galactose, labelling patterns did not vary from one morphological stage to another. Galactose, which was localized by using a monoclonal antibody to a galactose-specific lectin purified from insect larval hemolymph, was absent from cell walls of hyphal bodies and conidia but was present on germ-tube and mycelial surfaces. Chitin (N-acetylglucosamine), labelled with a wheat-germ

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