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1

L., Rizzo, Manaia C., Merlin C., et al. "Urban wastewater treatment plants as hotspots for antibiotic resistant bacteria and genes spread into the environment: A review." Science of the Total Environment 447 (February 7, 2013): 345–60. https://doi.org/10.1016/j.scitotenv.2013.01.032.

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Urban wastewater treatment plants (UWTPs) are among the main sources of antibiotics' release into the environment. The occurrence of antibiotics may promote the selection of antibiotic resistance genes (ARGs) and antibiotic resistant bacteria (ARB), which shade health risks to humans and animals. In this paper the fate of ARB and ARGs in UWTPs, focusing on different processes/technologies (i.e., biological processes, advanced treatment technologies and disinfection), was critically reviewed. The mechanisms by which biological processes influence the development/selection of ARB and ARGs tr
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Xi, Chuanwu, Yongli Zhang, Carl F. Marrs, et al. "Prevalence of Antibiotic Resistance in Drinking Water Treatment and Distribution Systems." Applied and Environmental Microbiology 75, no. 17 (2009): 5714–18. http://dx.doi.org/10.1128/aem.00382-09.

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ABSTRACT The occurrence and spread of antibiotic-resistant bacteria (ARB) are pressing public health problems worldwide, and aquatic ecosystems are a recognized reservoir for ARB. We used culture-dependent methods and quantitative molecular techniques to detect and quantify ARB and antibiotic resistance genes (ARGs) in source waters, drinking water treatment plants, and tap water from several cities in Michigan and Ohio. We found ARGs and heterotrophic ARB in all finished water and tap water tested, although the amounts were small. The quantities of most ARGs were greater in tap water than in
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Xiao, Qingshan, Xin Wang, Chongxin Xu, Wei Chen, Qianchi Huang, and Xin Wang. "Spatial Distribution and Seasonal Variation of Antibiotic-Resistant Bacteria in an Urban River in Northeast China." Water 16, no. 9 (2024): 1268. http://dx.doi.org/10.3390/w16091268.

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As the largest freshwater river flowing through Harbin, the Songhua River is a standby water source. It is very important to know the species and distribution of antibiotic-resistant bacteria (ARB) in the river. In this study, five antibiotics were selected to screen and identify ARB in spring and autumn. The results showed that the concentration of cefotaxime-resistant bacteria was the highest, and the maximum concentration at S6 in spring was up to 1.40 × 104 CFU/mL. In spring and autumn, bacteria resistant to three antibiotics were screened at S1 of the Songhua River, and bacteria resistant
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Kimbell, Lee K., Emily Tummons, and Patrick J. McNamara. "Effects of Corrosion Inhibitors on Antibiotic Resistance in Drinking Water Distribution Systems." Journal AWWA 117, no. 3 (2025): 22–30. https://doi.org/10.1002/awwa.2417.

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Key TakeawaysAntibiotic‐resistant bacteria (ARB) and antibiotic resistance genes (ARGs) in environments such as drinking water distribution systems (DWDS) could increase the likelihood of pathogenic antibiotic resistance.A recent study aimed to quantify effects of three corrosion inhibitors on bacterial antibiotic‐ and metal‐resistance abundance in a source water serving several water treatment plants.Study results suggested that corrosion inhibitors such as orthophosphates likely have a significant role in bacterial communities and the prevailing levels of ARB and ARGs in DWDS.
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Zhang, Minglu, Mengyao Xu, Shaofeng Xu, et al. "Response of the Bacterial Community and Antibiotic Resistance in Overnight Stagnant Water from a Municipal Pipeline." International Journal of Environmental Research and Public Health 17, no. 6 (2020): 1995. http://dx.doi.org/10.3390/ijerph17061995.

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Although drinking water safety has raised considerable concern, to date, the hidden health risks in newly released overnight water from a municipal pipeline have seldom received attention. In this study, bacterial community composition and the response of antibiotic-resistant bacteria (ARB) to ciprofloxacin, azithromycin, tetracycline, penicillin, and cephalosporin in overnight stagnant water were analyzed. With increases in heterotrophic bacteria plate count (HPC) during water stagnation, the numbers of ARB and the ARB/HPC ratios for the five antibiotics in resident water were observed to inc
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Courti, Ibtissam, Cristina Muja, Thomas Maho, Florent P. Sainct, and Philippe Guillot. "Degradation of Bacterial Antibiotic Resistance Genes during Exposure to Non-Thermal Atmospheric Pressure Plasma." Antibiotics 11, no. 6 (2022): 747. http://dx.doi.org/10.3390/antibiotics11060747.

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Bacterial resistance to antibiotics has become a major public health problem in recent years. The occurrence of antibiotics in the environment, especially in wastewater treatment plants, has contributed to the development of antibiotic-resistant bacteria (ARB) and the spread of antibiotic resistance genes (ARGs). Despite the potential of some conventional processes used in wastewater treatment plants, the removal of ARB and ARGs remains a challenge that requires further research and development of new technologies to avoid the release of emerging contaminants into aquatic environments. Non-the
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Yuan, Xia, Ziqing Lv, Zeyu Zhang, Yu Han, Zhiquan Liu, and Hangjun Zhang. "A Review of Antibiotics, Antibiotic Resistant Bacteria, and Resistance Genes in Aquaculture: Occurrence, Contamination, and Transmission." Toxics 11, no. 5 (2023): 420. http://dx.doi.org/10.3390/toxics11050420.

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Antibiotics are commonly used to prevent and control diseases in aquaculture. However, long-term/overuse of antibiotics not only leaves residues but results in the development of antibiotic resistant bacteria (ARB) and antibiotic resistance genes (ARGs). Antibiotics, ARB, and ARGs are widespread in aquaculture ecosystems. However, their impacts and interaction mechanisms in biotic and abiotic media remain to be clarified. In this paper, we summarized the detection methods, present status, and transfer mechanisms of antibiotics, ARB, and ARGs in water, sediment, and aquaculture organisms. Curre
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Fusaro, Carmine, Valentina Miranda-Madera, Nancy Serrano-Silva, et al. "Antibiotic-Resistant Bacteria Isolated from Street Foods: A Systematic Review." Antibiotics 13, no. 6 (2024): 481. http://dx.doi.org/10.3390/antibiotics13060481.

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Street food may be a vehicle of antibiotic-resistant bacteria (ARB) and antibiotic resistance genes (ARGs) to humans. Foods contaminated with ARB entail serious problems or challenges in the fields of medical care, animal husbandry, food industry, and public health worldwide. The objectives of this systematic review were to identify and evaluate scientific reports associated with ARB isolated from various street foods. “Preferred reporting items for systematic reviews and meta-analysis” (PRISMA) guidelines were followed. The bibliographic material covers a period from January 2015 to April 202
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Yang, Fang, Yu Wang, Qianwen Liu, et al. "High Prevalence and Varied Distribution of Antibiotic-Resistant Bacteria in the Rhizosphere and Rhizoplane of Citrus medica." Microorganisms 10, no. 9 (2022): 1708. http://dx.doi.org/10.3390/microorganisms10091708.

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The plant-associated bacteria, including that in the rhizosphere and rhizoplane, play important roles in human exposure to antibiotic-resistant bacteria (ARB). The rhizosphere and rhizoplane represent two distinct environments with different selective pressures for bacterial colonization. However, whether the difference in characteristics between the rhizosphere and rhizoplane can affect the abundance and antibiotic resistance profiles of ARB colonizing, the two environments remain largely unknown. In this study, we obtained 174 bacterial isolates from the rhizosphere (113 isolates) and rhizop
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Prakash, E. Akhil, Tereza Hromádková, T. Jabir, et al. "Dissemination of multidrug resistant bacteria to the polar environment - Role of the longest migratory bird Arctic tern (Sterna paradisaea)." Science of The Total Environment 815 (April 1, 2022): 152727. https://doi.org/10.5281/zenodo.5911068.

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<strong>Highlights</strong> &bull; AMR was observed in the bacteria from the migratory bird, Arctic tern. &bull; Twelve genera of bacteria detected, dominated by&nbsp;<em>Staphylococcus</em>&nbsp;and&nbsp;<em>Aerococcus</em>. &bull;&nbsp;Highest ARB observed against &beta;-lactam and quinolone class of antibiotics. &bull;&nbsp;Birds sex influence the profile of antibiotic resistance. &nbsp; <strong>Abstract</strong> The ever-increasing prevalence of antibiotic-resistant bacteria(ARB), primarily due to the frequent use and misuse of antibiotics, is an issue of serious global concern. Migratory
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SIEDLECKA, AGATA, MIRELA WOLF-BACA, and KATARZYNA PIEKARSKA. "Antibiotic and Disinfectant Resistance in Tap Water Strains – Insight into the Resistance of Environmental Bacteria." Polish Journal of Microbiology 70, no. 1 (2021): 57–67. http://dx.doi.org/10.33073/pjm-2021-004.

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Although antibiotic-resistant bacteria (ARB) have been isolated from tap water worldwide, the knowledge of their resistance patterns is still scarce. Both horizontal and vertical gene transfer has been suggested to contribute to the resistance spread among tap water bacteria. In this study, ARB were isolated from finished water collected at two independent water treatment plants (WTPs) and tap water collected at several point-of-use taps during summer and winter sampling campaigns. A total of 24 strains were identified to genus or species level and subjected to antibiotic and disinfectant susc
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JAGA, IOANA MIHAELA, ALINA MANOLE, ECATERINA SÂRBU, et al. "The contribution of manure to antibiotic resistance and the spread of antibiotic resistance genes in soil: a review." Romanian Biotechnological Letters 27, no. 2/2022 (2022): 3352–61. http://dx.doi.org/10.25083/rbl/27.2/3352.3361.

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The intensive use of antibiotics, worldwide, in animal husbandry, has led to the development and enrichment of different environments in antibiotic-resistant bacteria (ARB) and antibiotic-resistance genes (ARGs). Moreover, the subsequent application of manure contributes to the emergence of antimicrobial resistance (AMR) in soil. The spread of ARB and ARGs through trophic networks and potential human transmission indicate the need for innovative treatment approaches and strategies to reduce manure contaminants.
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Li, Yongqiang, Chongmiao Zhang, Xiao Mou, Peipei Zhang, Jie Liang, and Zhen Wang. "Distribution characteristics of antibiotic resistance bacteria and related genes in urban recreational lakes replenished by different supplementary water source." Water Science and Technology 85, no. 4 (2022): 1176–90. http://dx.doi.org/10.2166/wst.2022.018.

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Abstract The distribution characteristics of antibiotic-resistant bacteria (ARB) and antibiotic resistance genes (ARGs) in urban recreational water from different water-supply sources might be different. In this study, water samples were collected to detect the antibiotic resistance of heterotrophic bacteria to five antibiotics, and the content, phenotype, gene type and species distribution of resistant bacteria were analyzed. The results showed that the changes of bacteria resistance rate in two lakes to five kinds of antibiotics were synchronous with time, and it would reach its maximum in a
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Shang, Ming, Yongchao Gao, Liwen Zheng, et al. "Vertical Distribution and Drivers of Antibiotic Resistance Genes in Agricultural Soil Irrigated with Livestock Wastewater." Microorganisms 13, no. 3 (2025): 610. https://doi.org/10.3390/microorganisms13030610.

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Livestock wastewater reuse could be a potential source for the distribution of antibiotics, antibiotic resistance bacteria (ARB), and antibiotic resistance genes (ARGs) in agricultural soil. In this study, soil samples were collected from different depths (0–60 cm) of farmland that has been subjected to long-term application of livestock wastewater. The vertical distribution of antibiotics, bacterial communities, and ARGs were assessed to identify the driving factors that could potentially influence the distribution of ARB and ARGs. The results demonstrated distinguished distributions of antib
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Ilmi, Akna Mumtaz, Farah Adiba, Kirana Anisah, et al. "Removal of antibiotic resistant bacteria in wastewater treatment plants." E3S Web of Conferences 485 (2024): 04006. http://dx.doi.org/10.1051/e3sconf/202448504006.

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Antibiotic Resistant Bacteria (ARB) emergence is an increasing threat to public health globally. Wastewater Treatment Plants (WWTPs) play a crucial role in the dissemination of ARB in the environment due to limitations in ARB removal. This study investigated the presence and characteristics of ARB, specifically Extended-Spectrum Beta-Lactamase producing Escherichia coli (ESBL-Ec) and Vancomycin Resistant Enterococci (VRE) , in two WWTPs treating wastewater from hospital and domestic source. The study employed phenotypic and genotypic tests to confirm the presence of ESBL-Ec and VRE, and evalua
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Kwok, Annie, Michael C. Davis, and Sanghoon Kang. "Antibiotic resistant bacteria in an urban freshwater ecosystem in central Texas." Fine Focus 3, no. 2 (2017): 113–26. http://dx.doi.org/10.33043/ff.3.2.113-126.

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Antibiotic resistance is a growing concern for the human population and is becoming prevalent in many environments. For example, increasing occurrences of antibiotic resistance genes (ARGs) in aquatic ecosystems elevates the risk of pathogenic microbes acquiring those resistance genes. There is an urgent need to more closely examine the relationship between antibiotic resistant bacteria (ARB) and antibiotic residues in urban freshwater environments. Thus, our main objective was to investigate the presence of antibiotic resistance in wastewater treatment plant (WWTP) influent and effluent leadi
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Ye, Jinzhou, and Xinhai Chen. "Current Promising Strategies against Antibiotic-Resistant Bacterial Infections." Antibiotics 12, no. 1 (2022): 67. http://dx.doi.org/10.3390/antibiotics12010067.

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Infections caused by antibiotic-resistant bacteria (ARB) are one of the major global health challenges of our time. In addition to developing new antibiotics to combat ARB, sensitizing ARB, or pursuing alternatives to existing antibiotics are promising options to counter antibiotic resistance. This review compiles the most promising anti-ARB strategies currently under development. These strategies include the following: (i) discovery of novel antibiotics by modification of existing antibiotics, screening of small-molecule libraries, or exploration of peculiar places; (ii) improvement in the ef
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Potorski, Jacek, Izabela Koniuszewska, Małgorzata Czatzkowska, and Monika Harnisz. "Drug resistance in airborne bacteria isolated from waste management and wastewater treatment plants in Olsztyn." E3S Web of Conferences 100 (2019): 00066. http://dx.doi.org/10.1051/e3sconf/201910000066.

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Wastewater treatment plants (WWTPs) and municipal waste management plants (MWMPs) emit bioaerosols containing potentially pathogenic biological components which post a threat for human health. Microbiological monitoring supports evaluations of the antibiotic resistance (AR) of airborne microorganisms and the relevant health risks. The aim of this study was to analyze the microbiological quality of air sampled in a WWTP and MWMP in Olsztyn based on total bacterial counts, the presence of bacteria resistant to three antibiotic classes (beta-lactams, tetracyclines and chloramphenicol) and genes e
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Azizi, Mounia, and Souhail Mouline. "Bacterial Resistance in Nephrology." Scholars Journal of Applied Medical Sciences 12, no. 01 (2024): 79–84. http://dx.doi.org/10.36347/sjams.2024.v12i01.014.

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Bacterial resistance to antibiotics (ABR) is a major threat to public health, particularly in nephrology, with far-reaching consequences, including longer hospital stays, higher healthcare costs and increased mortality. Indeed, patients with chronic kidney disease (CKD) are a population at risk of developing infections caused by antibiotic-resistant bacteria (ARBs), given their overexposure to healthcare facilities and the quality of their gut microbiota already damaged by CKD. It is a population with very high rates of colonization and ARB infection worldwide. The mechanisms deployed by these
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Obayiuwana, Amarachukwu, and Abasiofiok M. Ibekwe. "Antibiotic Resistance Genes Occurrence in Wastewaters from Selected Pharmaceutical Facilities in Nigeria." Water 12, no. 7 (2020): 1897. http://dx.doi.org/10.3390/w12071897.

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The proliferation of antibiotic-resistant bacteria (ARB) and the prevalence of antibiotic resistance genes (ARGs) in wastewaters are well-established factors that contribute to the reduced potency of antibiotics used in healthcare worldwide. The human health risk associated with the proliferation of ARB and ARGs need to be understood in order to design mitigation measures to combat their dissemination. Using the PCR analysis of genomic DNA, the prevalence of 41 ARGs active against the commonly used six classes of antibiotics was evaluated in 60 bacterial isolates obtained from pharmaceutical w
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Scott, Laura C., Nicholas Lee, and Tiong Gim Aw. "Antibiotic Resistance in Minimally Human-Impacted Environments." International Journal of Environmental Research and Public Health 17, no. 11 (2020): 3939. http://dx.doi.org/10.3390/ijerph17113939.

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Antibiotic resistant bacteria (ARB) have become contaminants of concern in environmental systems. Studies investigating environmental ARB have primarily focused on environments that are greatly impacted by anthropogenic activity. Background concentrations of ARB in natural environments is not well understood. This review summarizes the current literature on the monitoring of ARB and antibiotic resistance genes (ARGs) in environments less impacted by human activity. Both ARB and ARGs have been detected on the Antarctic continent, on isolated glaciers, and in remote alpine environments. The meth
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Nkamguia, Loïc Kevin Kamgaing, O’Neal Dorsel Youté, Blandine Pulcherie Tamatcho Kweyang, et al. "Antibiotics Resistance and Abundance of Some Bacteria from Agricultural and Non-agricultural Soil in One of the Most Agriculturally-active Settings in West Cameroon." Journal of Advances in Microbiology 24, no. 12 (2024): 244–54. https://doi.org/10.9734/jamb/2024/v24i12887.

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Background: Agriculture is also concerned with the problem of bacterial resistance because agricultural soils are reservoirs of antibiotic-resistant bacteria (ARB) and antibiotic-resistant genes. Objective: An investigation about ARB was carried out on agricultural soils in Mangoum, a neighborhood of the Foumbot municipality (Noun division, West Cameroon). Methods: It was conducted as a cross-sectional descriptive study with a total of 46 soil specimens collected from plant farms and a control plot. Isolation, enumeration and antibiotic susceptibility tests were performed according to standard
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Bukha, Khawla K., Nouralhuday A. Altayr, Samira A. Shlayek, and Ibrahim M. Eldaghayes. "The Association between the Global Threat of Ocean Pollution and Climate Change on the Distribution of Antibiotic Resistance: One Health Strategy." World's Veterinary Journal 15, no. 1 (2025): 194–214. https://doi.org/10.54203/scil.2025.wvj22.

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Antibiotic resistance represents a fundamental issue affecting public health today. Antibiotic resistance occurs when bacteria alter their response to antibiotics. Pathogenic bacteria and their genes can diffuse throughout human and natural habitats. The complicated interactions between diverse bacterial communities that affect the health of people, aquatic animals, and the aquatic environment are an illustration of ecological issues. Pollution of the oceans by antibiotic-resistant bacteria (ARB) can disturb the natural equilibrium of the oceans and may spread to humans. Also, climate change (
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Fan, Shi. "On the Generation, Impact and Removal of Antibiotic Resistance in the Water Environment." BIO Web of Conferences 59 (2023): 02008. http://dx.doi.org/10.1051/bioconf/20235902008.

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Emerging pollutants that have the potential to significantly impact the environment include antibiotics. Antibiotic resistance, or microorganisms' ability to withstand medications intended to kill them, can have an impact on a variety of facets of daily life. One of the most vital resources for life, water, contains antibiotic resistance. This review explains where antibiotics and antibiotic resistance genes come from, how they contaminate the aquatic environment, and how they get into water bodies. Since wastewater is the most prominent growth environment for the production and amplification
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Chatterjee, Rudrangshu, Dushyant Singh, M. L. Aggarwal, Ajit Varma, Abhishek Chauhan, and Swati Tripathi. "Assessment of Murraya koenigii Leaf Extract against New Multiple Drug Resistance Human Enteric Pathogens." Asian Journal of Chemistry 32, no. 10 (2020): 2647–52. http://dx.doi.org/10.14233/ajchem.2020.22869.

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Sewage waters are the primary habitats to harbour antibiotic resistance bacteria (ARB) especially multi-drug resistance (MDR) human enteric pathogens. Microorganisms acquire resistance towards many commercial antibiotics due to their inappropriate use. In this study, human enteric pathogens were isolated, identified and characterized and shows the resistance against five different clinically significant commonly prescribed antibiotics. The bacterial strains were isolated from different sewage treatment plants located in Delhi city, India. Samples were analyzed for the detection of pathogenic h
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Sorgen, Alicia, James Johnson, Kevin Lambirth, et al. "Characterization of Environmental and Cultivable Antibiotic-Resistant Microbial Communities Associated with Wastewater Treatment." Antibiotics 10, no. 4 (2021): 352. http://dx.doi.org/10.3390/antibiotics10040352.

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Bacterial resistance to antibiotics is a growing global concern, threatening human and environmental health, particularly among urban populations. Wastewater treatment plants (WWTPs) are thought to be “hotspots” for antibiotic resistance dissemination. The conditions of WWTPs, in conjunction with the persistence of commonly used antibiotics, may favor the selection and transfer of resistance genes among bacterial populations. WWTPs provide an important ecological niche to examine the spread of antibiotic resistance. We used heterotrophic plate count methods to identify phenotypically resistant
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Siedlecka, Agata, Mirela Wolf-Baca, and Katarzyna Piekarska. "Spatiotemporal Changes of Antibiotic Resistance and Bacterial Communities in Drinking Water Distribution System in Wrocław, Poland." Water 12, no. 9 (2020): 2601. http://dx.doi.org/10.3390/w12092601.

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Antibiotic resistance of bacteria is an emerging problem in drinking water treatment. This paper presents the comparison of antibiotic resistant bacteria (ARB) and antibiotic resistance genes (ARGs) prevalence during the summer and winter season in a full-scale drinking water distribution system (DWDS) supplied by two water treatment plants (WTPs). The effect of distance from WTP and physical–chemical water parameters on its microbial properties was also tested. Bacterial consortia dwelling in bulk tap water were additionally compared by means of denaturating gradient gel electrophoresis (DGGE
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McNulty, Cliodna, Brieze Read, Anna Quigley, Neville Q. Verlander, and Donna M. Lecky. "What the public in England know about antibiotic use and resistance in 2020: a face-to-face questionnaire survey." BMJ Open 12, no. 4 (2022): e055464. http://dx.doi.org/10.1136/bmjopen-2021-055464.

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ObjectivesTo describe public attitudes and knowledge around antibiotic activity, resistance and use.DesignFace-to-face household 18 question survey using computer-assisted data collection undertaken by Ipsos Market and Opinion Research International.SettingRandomly selected households across England, January–February 2020.Participants2022 adults (aged 15+,) including 521 black, Asian and minority ethnic (BAME) participants, and 406 aged 15–25 years olds.Main outcome measuresResponses to questions about antibiotic activity, resistance and expectations for antibiotics and trust in healthcare pro
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Karungamye, Petro, Anita Rugaika, Kelvin Mtei, and Revocatus Machunda. "Antibiotic Resistance Patterns of Escherichia coli, Klebsiella pneumoniae, and Pseudomonas aeruginosa Isolated from Hospital Wastewater." Applied Microbiology 3, no. 3 (2023): 867–82. http://dx.doi.org/10.3390/applmicrobiol3030060.

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Antibiotic-resistant bacteria (ARB) and antibiotic resistance genes (ARGs) in treated hospital wastewater effluents constitute a major environmental and public health concern. The aim of this study was to investigate the antibiotic resistance patterns of Escherichia coli, Klebsiella pneumoniae, and Pseudomonas aeruginosa isolated from wastewater effluent at the Benjamin Mkapa Hospital (BMH) in Dodoma, Tanzania. These bacteria were selected to represent the most prevalent gram-negative bacteria found in hospital wastewater, and they have the potential to generate resistance and spread resistanc
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Caruso, Gabriella, Maurizio Azzaro, Ombretta Dell’Acqua, Maria Papale, Angelina Lo Giudice, and Pasqualina Laganà. "Plastic Polymers and Antibiotic Resistance in an Antarctic Environment (Ross Sea): Are We Revealing the Tip of an Iceberg?" Microorganisms 12, no. 10 (2024): 2083. http://dx.doi.org/10.3390/microorganisms12102083.

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Microbial colonization of plastic polymers in Antarctic environments is an under-investigated issue. While several studies are documenting the spread of plastic pollution in the Ross Sea, whether the formation of a plastisphere (namely the complex microbial assemblage colonizing plastics) may favor the spread of antibiotic-resistant bacteria (ARB) in this marine environment is unknown yet. A colonization experiment was performed in this ecosystem, aiming at exploring the potential role of plastic polymers as a reservoir of antibiotic resistance. To this end, the biofilm-producing activity and
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Gao, Rui, and Minghao Sui. "Antibiotic resistance fate in the full-scale drinking water and municipal wastewater treatment processes: A review." Environmental Engineering Research 26, no. 4 (2020): 200324–0. http://dx.doi.org/10.4491/eer.2020.324.

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Antibiotic-resistant bacteria (ARB) and antibiotic resistance genes (ARGs) have been widely identified as emerging pollutants in various aquatic compartments. Concerns have been raised that the antibiotic resistance determinants may occur in treated drinking water and wastewater, weakening the therapeutic efficacy of antibiotics and so posing threat to public health. Most often, laboratory trials are conducted to assess the removal efficiency of ARB and ARGs in individual treatment processes (e.g., disinfection). However, the random variations of microbial distribution and chemical composition
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Almeida, Ana Rita, Marta Tacão, Joana Soares, Inês Domingues, and Isabel Henriques. "Tetracycline-Resistant Bacteria Selected from Water and Zebrafish after Antibiotic Exposure." International Journal of Environmental Research and Public Health 18, no. 6 (2021): 3218. http://dx.doi.org/10.3390/ijerph18063218.

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The emergence of antibiotic-resistant pathogens due to worldwide antibiotic use is raising concern in several settings, including aquaculture. In this work, the selection of antibiotic-resistant bacteria (ARB) and antibiotic resistance genes (ARGs) was evaluated after exposure of zebrafish to oxytetracycline (OTC) for two months, followed by a recovery period. The selection of ARB in water and fish was determined using selective media. The abundance of tetA genes was estimated through qPCR. Higher prevalence of ARB was measured in all samples exposed to the antibiotic when compared to control
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Laws, Mark, Ali Shaaban, and Khondaker Miraz Rahman. "Antibiotic resistance breakers: current approaches and future directions." FEMS Microbiology Reviews 43, no. 5 (2019): 490–516. http://dx.doi.org/10.1093/femsre/fuz014.

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ABSTRACTInfections of antibiotic-resistant pathogens pose an ever-increasing threat to mankind. The investigation of novel approaches for tackling the antimicrobial resistance crisis must be part of any global response to this problem if an untimely reversion to the pre-penicillin era of medicine is to be avoided. One such promising avenue of research involves so-called antibiotic resistance breakers (ARBs), capable of re-sensitising resistant bacteria to antibiotics. Although some ARBs have previously been employed in the clinical setting, such as the β-lactam inhibitors, we posit that the br
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Zhao, Fuzheng, Qingmiao Yu, and Xu-Xiang Zhang. "A Mini-Review of Antibiotic Resistance Drivers in Urban Wastewater Treatment Plants: Environmental Concentrations, Mechanism and Perspectives." Water 15, no. 17 (2023): 3165. http://dx.doi.org/10.3390/w15173165.

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Antibiotic resistance is one of the biggest challenges to public health and ecological safety in the 21st century. Urban wastewater treatment plants (UWTPs), as reservoirs of antibiotic resistance genes (ARGs) and antibiotic-resistant bacteria (ARB), simultaneously contain a wide variety of chemical pollutants. The review introduces the actual concentration levels and the mechanisms of antibiotic resistance drivers (ARDs) in UWTPs, including antibiotics, heavy metals, disinfectants, cosmetics and personal care products, non-antibiotic drugs, and microplastics. Moreover, this review emphasizes
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Chen, Lei, Zhi Zhou, Chaofeng Shen, and Yilu Xu. "Inactivation of antibiotic-resistant bacteria and antibiotic resistance genes by electrochemical oxidation/electro-Fenton process." Water Science and Technology 81, no. 10 (2020): 2221–31. http://dx.doi.org/10.2166/wst.2020.282.

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Abstract Antibiotic-resistant bacteria (ARB) and antibiotic resistance genes (ARGs) in the environment are of great concern due to their potential risk to human health. The effluents from wastewater treatment plants and livestock production are major sources of ARB and ARGs. Chlorination, UV irradiation, and ozone disinfection cannot remove ARGs completely. In this study, the potential of electrochemical oxidation and electro-Fenton processes as alternative treatment technologies for inactivation of ARB and ARGs in both intracellular and extracellular forms was evaluated. Results showed that t
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Cho, Sohyun, Lari M. Hiott, Quentin D. Read, et al. "Distribution of Antibiotic Resistance in a Mixed-Use Watershed and the Impact of Wastewater Treatment Plants on Antibiotic Resistance in Surface Water." Antibiotics 12, no. 11 (2023): 1586. http://dx.doi.org/10.3390/antibiotics12111586.

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The aquatic environment has been recognized as a source of antibiotic resistance (AR) that factors into the One Health approach to combat AR. To provide much needed data on AR in the environment, a comprehensive survey of antibiotic-resistant bacteria (ARB), antibiotic resistance genes (ARGs), and antibiotic residues was conducted in a mixed-use watershed and wastewater treatment plants (WWTPs) within the watershed to evaluate these contaminants in surface water. A culture-based approach was used to determine prevalence and diversity of ARB in surface water. Low levels of AR Salmonella (9.6%)
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Kong, Cen, Xin He, Meiting Guo, Shunjun Ma, Bin Xu, and Yulin Tang. "The Impacts of Chlorine and Disinfection Byproducts on Antibiotic-Resistant Bacteria (ARB) and Their Conjugative Transfer." Water 14, no. 19 (2022): 3009. http://dx.doi.org/10.3390/w14193009.

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Antibiotic-resistant bacteria (ARB) and antibiotic resistance genes (ARGs) are emerging contaminants leading to severe worldwide health problems. Chlorination, a widely used procedure, was extensively explored as one of the main methods to remove ARB and ARGs in recent years. In this study, to enrich the analyses of chlorination, several comprehensive effects of the chlorine disinfection system on ARB and their conjugative transfer ability were explored. The results presented that the low dose of chlorine (&lt;3-log inactivation rate) had little influence on the survival of bacteria in terms o
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Aguilar-Salazar, Alejandra, Ana Verónica Martínez-Vázquez, Guadalupe Aguilera-Arreola, et al. "Prevalence of ESKAPE Bacteria in Surface Water and Wastewater Sources: Multidrug Resistance and Molecular Characterization, an Updated Review." Water 15, no. 18 (2023): 3200. http://dx.doi.org/10.3390/w15183200.

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ESKAPE bacteria represent a group of opportunistic bacterial pathogens that display widespread antimicrobial resistance, including resistance to the last-line antibiotics, thereby posing a significant clinical implication globally. Anthropogenic activities, such as wastewater from hospitals, livestock farms, crop fields, and wastewater treatment plants, contribute to the dissemination of antimicrobial-resistant bacterial pathogens into the environment. Surface water sources, including river waters, act as critical points of discharge for wastewater, pollutants, antibiotic-resistant bacteria (A
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Triggiano, Francesco, Carla Calia, Giusy Diella, Maria Teresa Montagna, Osvalda De Giglio, and Giuseppina Caggiano. "The Role of Urban Wastewater in the Environmental Transmission of Antimicrobial Resistance: The Current Situation in Italy (2010–2019)." Microorganisms 8, no. 10 (2020): 1567. http://dx.doi.org/10.3390/microorganisms8101567.

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Scientific studies show that urban wastewater treatment plants (UWWTP) are among the main sources of release of antibiotics, antibiotic resistance genes (ARG) and antibiotic-resistant bacteria (ARB) into the environment, representing a risk to human health. This review summarizes selected publications from 1 January 2010 to 31 December 2019, with particular attention to the presence and treatment of ARG and ARB in UWWTPs in Italy. Following a brief introduction, the review is divided into three sections: (i) phenotypic assessment (ARB) and (ii) genotypic assessment (ARG) of resistant microorga
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Buriánková, Iva, Peter Kuchta, Anna Molíková, et al. "Antibiotic Resistance in Wastewater and Its Impact on a Receiving River: A Case Study of WWTP Brno-Modřice, Czech Republic." Water 13, no. 16 (2021): 2309. http://dx.doi.org/10.3390/w13162309.

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Antibiotic resistance has become a global threat in which the anthropogenically influenced aquatic environment represents not only a reservoir for the spread of antibiotic resistant bacteria (ARB) among humans and animals but also an environment where resistance genes are introduced into natural microbial ecosystems. Wastewater is one of the sources of antibiotic resistance. The aim of this research was the evaluation of wastewater impact on the spread of antibiotic resistance in the water environment. In this study, qPCR was used to detect antibiotic resistance genes (ARGs)—blaCTX-M-15, blaCT
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Tsvetanova, Zvezdimira, Iva Tsvetkova, and Hristo Najdenski. "Antimicrobial Resistance of Heterotrophic Bacteria in Drinking Water-Associated Biofilms." Water 14, no. 6 (2022): 944. http://dx.doi.org/10.3390/w14060944.

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Antimicrobial resistance (AMR) is one of the major threats to human health and is becoming an environmental challenge for water resources too. Our study’s aim was: to assess the AMR of heterotrophic bacteria in drinking water-associated biofilms against six clinically important antibiotics; to compare the prevalence of antibiotic resistant bacteria (ARB) in drinking water and in the associated biofilms; to estimate biofilm formation ability of selected isolates. Culture-dependent methods were used in the population-based study of the biofilms and in assessment of the single-species biofilm for
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Hobeika, Wadad, Margaux Gaschet, Marie-Cécile Ploy, Elena Buelow, Dolla Karam Sarkis, and Christophe Dagot. "Resistome Diversity and Dissemination of WHO Priority Antibiotic Resistant Pathogens in Lebanese Estuaries." Antibiotics 11, no. 3 (2022): 306. http://dx.doi.org/10.3390/antibiotics11030306.

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Anthropogenic pressure is known to be a key driver of antimicrobial resistance (AMR) dissemination in the environment. Especially in lower income countries, with poor infrastructure, the level of AMR dissemination is high. Therefore, we assessed the levels and diversity of antibiotic-resistant bacteria (ARB) and antibiotic resistance genes (ARGs) in Lebanese rivers at estuaries’ sites (n = 72) of the Mediterranean Sea in spring 2017 and winter 2018. Methods: A combined approach using culture techniques and high throughput qPCR were applied to identify ARB and ARGs in rivers along the Lebanese
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Hou, Xuan, Jie Yang, Jinjing Xie, Shaowei Zhu, and Zhenfen Zhang. "Diversity and Antibiotic Resistance of Triticale Seed-Borne Bacteria on the Tibetan Plateau." Microorganisms 12, no. 4 (2024): 650. http://dx.doi.org/10.3390/microorganisms12040650.

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The Tibetan Plateau is located in southwestern China. It has many important ecological functions, such as biodiversity protection, and is an important grassland agroecosystem in China. With the development of modern agriculture and animal husbandry, antibiotics are widely used to treat humans and livestock, and antibiotics cannot be fully metabolised by both. Antibiotics eventually find their way into the environment, affecting other parts of grassland agroecosystems. Triticale (Triticosecale wittmack) is an artificial hybrid forage that can be used for both grain and forage. This study reveal
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Muthoni, Esther, Kevin Mbogo, Christine Mutoro, Dorothy Kagendo, and Victor Mwiti Marangu. "Surveillance of Antibiotic Resistance in Gram-Negative Bacteria from Industrial Wastewater in Nairobi." Chemical Science International Journal 34, no. 3 (2025): 38–46. https://doi.org/10.9734/csji/2025/v34i3966.

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The environmental dispersal of antibiotic resistant bacteria (ARB) and antibiotic resistance genes (ARGs) represents an increasing global public health concern. This challenge is especially pronounced in low and middle-income countries where industrial wastewater management remains inadequate. Industrial wastewater serves as a reservoir of antibiotic resistant microorganisms. These microorganisms facilitate the transfer of resistance traits within natural ecosystems and ultimately to human populations through direct contact, the food chain or through contaminated water sources. The present stu
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Aali, Rahim, Sepideh Baragh, Esrafil Asgari, et al. "Tracking of chloramphenicol, erythromycin, and sulfamethoxazole antibiotic-resistant bacteria from untreated wastewater effluents to receiving river." Environmental Health Engineering and Management 6, no. 2 (2019): 89–96. http://dx.doi.org/10.15171/ehem.2019.10.

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Background: The aim of this study was to investigate the frequency and type of bacteria resistant to chloramphenicol (CHL), erythromycin (E), and sulfamethoxazole (SXT) antibiotics from untreated wastewater effluents to receiving river. Methods: In total, 32 samples were taken from eight sites located in the raw wastewater to the downstream of the receiving Ghotor river in Khoy city. Resistant microorganisms were studied through modified HPC method and CLSI standards. Different and specific colonies were selected and re-cultured in R2A culture medium and enough colonies were used for DNA extra
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Carstens, Alewyn, Catheleen Bartie, Rainier Dennis, and Carlos Bezuidenhout. "Antibiotic-resistant heterotrophic plate count bacteria and amoeba-resistant bacteria in aquifers of the Mooi River, North West province, South Africa." Journal of Water and Health 12, no. 4 (2014): 835–45. http://dx.doi.org/10.2166/wh.2014.226.

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Groundwater in the Mooi River catchment is prone to mining, agricultural, municipal and septic tank pollution. In this study physico-chemical and microbiological parameters were determined using appropriate methods. Bacterial isolates were identified by 16S rRNA sequencing (heterotrophic plate count (HPC) bacteria and amoeba-resistant bacteria (ARB)) and multiplex polymerase chain reaction (Escherichia coli). Antibiotic resistance tests were also performed. Physico-chemical parameters were generally within target water quality ranges for drinking water. HPC bacteria ranged between 105 and 107
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WU, JIAJIA, FAN YE, JIONG QU, and ZHIYUAN DAI. "Insight into the Antibiotic Resistance of Bacteria Isolated from Popular Aquatic Products Collected in Zhejiang, China." Polish Journal of Microbiology 72, no. 1 (2023): 61–67. http://dx.doi.org/10.33073/pjm-2023-010.

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Abstract The present study was aimed to obtain a close insight into the distribution and diversity of antibiotic-resistant bacteria (ARB) and antibiotic-resistance genes (ARGs) among the aquatic products collected in Zhejiang, China. A total of 136 presumptive ARB picked up from six aquatic samples were classified into 22 genera and 49 species based on the 16S rDNA sequencing. Aeromonas spp., Shewanella spp., Acinetobacter spp., Myroides spp., Pseudomonas spp., and Citrobacter spp. accounted for 80% of the ARB. Among them, 109 isolates (80.15%) exhibited resistance to at least one antibiotic.
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Zhao, Fuzheng, Bo Wang, Kailong Huang, et al. "Correlations among Antibiotic Resistance Genes, Mobile Genetic Elements and Microbial Communities in Municipal Sewage Treatment Plants Revealed by High-Throughput Sequencing." International Journal of Environmental Research and Public Health 20, no. 4 (2023): 3593. http://dx.doi.org/10.3390/ijerph20043593.

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Municipal sewage treatment plants (MSTPs) are environmental pools for antibiotic resistant bacteria (ARB) and antibiotic resistance genes (ARGs), which is cause for growing environmental-health concerns. In this study, the effects of different wastewater treatment processes on microbial antibiotic resistance in four MSTPs were investigated. PCR, q-PCR, and molecular cloning integrally indicated that the tetracycline resistance (tet) genes significantly reduced after activated-sludge treatment. Illumina high-throughput sequencing revealed that the broad-spectrum profile of ARGs and mobile eleme
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Lenart-Boroń, Anna, Klaudia Stankiewicz, Natalia Czernecka, et al. "Wounds of Companion Animals as a Habitat of Antibiotic-Resistant Bacteria That Are Potentially Harmful to Humans—Phenotypic, Proteomic and Molecular Detection." International Journal of Molecular Sciences 25, no. 6 (2024): 3121. http://dx.doi.org/10.3390/ijms25063121.

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Skin wounds and their infections by antibiotic-resistant bacteria (ARB) are very common in small animals, posing the risk of acquiring ARB by pet owners or antibiotic resistance gene (ARG) transfer to the owners’ microbiota. The aim of this study was to identify the most common pathogens infecting wounds of companion animals, assess their antibiotic resistance, and determine the ARGs using culture-based, molecular, and proteomic methods. A total of 136 bacterial strains were isolated from wound swabs. Their species was identified using chromogenic media, followed by MALDI-TOF spectrometry. Ant
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Kalli, Maria, Constantinos Noutsopoulos, and Daniel Mamais. "The Fate and Occurrence of Antibiotic-Resistant Bacteria and Antibiotic Resistance Genes during Advanced Wastewater Treatment and Disinfection: A Review." Water 15, no. 11 (2023): 2084. http://dx.doi.org/10.3390/w15112084.

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Antimicrobial resistance (AMR) is a serious problem for modern society, not only associated with clinical environments, but also the natural environment. Conventional wastewater treatment plants (WWTPs) are important nodes for the dissemination of antibiotic resistance to the aquatic environment since they are reservoirs of antibiotic-resistant bacteria (ARB), antibiotic resistance genes (ARGs), and antibiotic residues. WWTPs are not designed to remove these antibiotic resistance determinants from wastewater, and as a result, they are present in treated effluent, leading to environmental and p
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