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1

Lucena, Rafael, Noah Dephoure, Steve P. Gygi, et al. "Nucleocytoplasmic transport in the midzone membrane domain controls yeast mitotic spindle disassembly." Journal of Cell Biology 209, no. 3 (2015): 387–402. http://dx.doi.org/10.1083/jcb.201412144.

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During each cell cycle, the mitotic spindle is efficiently assembled to achieve chromosome segregation and then rapidly disassembled as cells enter cytokinesis. Although much has been learned about assembly, how spindles disassemble at the end of mitosis remains unclear. Here we demonstrate that nucleocytoplasmic transport at the membrane domain surrounding the mitotic spindle midzone, here named the midzone membrane domain (MMD), is essential for spindle disassembly in Schizosaccharomyces pombe cells. We show that, during anaphase B, Imp1-mediated transport of the AAA-ATPase Cdc48 protein at
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Su, Shi Jie, Xi Feng Fang, and Fang Li. "Research on the Disassembly Sequence Planning for Mechanical Product." Applied Mechanics and Materials 80-81 (July 2011): 1300–1304. http://dx.doi.org/10.4028/www.scientific.net/amm.80-81.1300.

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Mechanical products have been inevitably disassembled when they are regarded to be recycled or repaired. The disassembling procedure must be decided no matter what way is used to disassemble. The key is to decide the disassembly sequence for parts/components. In order to generate the disassembly sequence rapidly and correctly, similar thread binary tree and graph algorithm are used to establish the model of disassembly after analyzing the characteristics of Mechanical products. Firstly, generate the related matrix obtained from the 3D CAD assembly model; secondly, transform the undirected grap
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Swart, Arthur J. "Engaging African engineering students with problem-based learning by using the disassembly–assembly technique." International Journal of Electrical Engineering & Education 55, no. 3 (2018): 244–57. http://dx.doi.org/10.1177/0020720918767052.

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Problem-based learning identifies problems in ways that are conducive to student learning and emphasizes problem-solving, critical thinking and collaborative skills. These three aspects are core to disassembly–assembly techniques used in higher education where students are required to engage with visual, auditory and kinesthetic learning within a laboratory environment. The purpose of this article is to describe a disassembly–assembly technique used in a compulsory engineering module and identify how many African engineering students can successfully create a problem (disassemble a two-stroke
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4

Diepholz, Meikel, Michael Börsch, and Bettina Böttcher. "Structural organization of the V-ATPase and its implications for regulatory assembly and disassembly." Biochemical Society Transactions 36, no. 5 (2008): 1027–31. http://dx.doi.org/10.1042/bst0361027.

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V-ATPases (vacuolar ATPases) are membrane-bound multiprotein complexes that are localized in the endomembrane systems of eukaryotic cells and in the plasma membranes of some specialized cells. They couple ATP hydrolysis with the transport of protons across membranes. On nutrient shortage, V-ATPases disassemble into a membrane-embedded part (V0), which contains the proton translocation machinery, and an extrinsic part (V1), which carries the nucleotide-binding sites. Disassembly decouples ATP hydrolysis and proton translocation. Furthermore, the disassembled parts are inactive, leading to an ef
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5

Newman, Joseph, David J. Rowlands, and Tobias J. Tuthill. "An Engineered Maturation Cleavage Provides a Recombinant Mimic of Foot-and-Mouth Disease Virus Capsid Assembly-Disassembly." Life 11, no. 6 (2021): 500. http://dx.doi.org/10.3390/life11060500.

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Picornavirus capsids are assembled from 60 copies of a capsid precursor via a pentameric assembly intermediate or ‘pentamer’. Upon completion of virion assembly, a maturation event induces a final cleavage of the capsid precursor to create the capsid protein VP4, which is essential for capsid stability and entry into new cells. For the picornavirus foot-and-mouth disease virus (FMDV), intact capsids are temperature and acid-labile and can disassemble into pentamers. During disassembly, capsid protein VP4 is lost, presumably altering the structure and properties of the resulting pentamers. The
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6

Shetty, Devdas, and Ahad Ali. "A new design tool for DFA/DFD based on rating factors." Assembly Automation 35, no. 4 (2015): 348–57. http://dx.doi.org/10.1108/aa-11-2014-088.

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Purpose – The purpose of this paper is to develop a tool design for assembly and disassembly using rating factors. Design engineers need an automated tool to effectively analyze the ease of assembly and disassembly of the products or subassemblies. A good assembly design helps in easier disassembly and thus makes it easier to service, repair and maintain. Reuse and recycling aspects are given importance in the present days due to environmental regulations. Designers now use the life cycle design of the products. This creates an environment for the successful application of design for manufactu
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Javorova, Angela, Erika Hrušková, and Karol Velíšek. "Assembly and Disassembly via Automation Tools." Key Engineering Materials 467-469 (February 2011): 2066–71. http://dx.doi.org/10.4028/www.scientific.net/kem.467-469.2066.

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Assembly and disassembly is a new and also a rapid developed trend in manufacturing area. In the future the assembly and disassembly will be one inseparable part of manufacturing process. Especially automated disassembly is a technology, which is trying to satisfy needs and requirements. Many of special requirements are supported by international institutions, research programs and foundations. In our Institute of Manufacturing Systems and Applied Mechanics at Faculty of Materials Science and Technology the project named Intelligent assembly cell is realized. Within the frame of this project d
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Cao, Xiangang, Guoyin Chen, Mengzhen Zuo, Jiasong Zang, Peng Wang, and Xudong Wu. "The Structural Design and Optimization of a Railway Fastener Nut Disassembly and Assembly Machine." Machines 13, no. 4 (2025): 322. https://doi.org/10.3390/machines13040322.

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During the maintenance of railway fasteners, there are issues with the current nut disassembly and assembly operation, including low efficiency, heavy reliance on manual labor, and high physical strain. A mechanical device has been designed to move along the railway track while identifying and locating the center of the nut to perform disassembly and assembly operations. First, based on the nut disassembly and assembly process and the operating environment, the structure of the equipment was designed. This machine can simultaneously disassemble and assemble all the nuts on a single rail tie an
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Holubek, Radovan, Roman Ruzarovsky, and Karol Velíšek. "New Approach in Design of Automated Assembly Station for Disassembly Process." Applied Mechanics and Materials 421 (September 2013): 595–600. http://dx.doi.org/10.4028/www.scientific.net/amm.421.595.

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In this contribution is presented the new idea of another approach and application of designed and verified assembly station that can be used for disassembly process. Automated assembly station with Cartesian industrial robot is especially designed for assembly of specific products. New approach consists in the fact that such equipment can be used for disassembly of assembled products after lifecycle. This solution can be used where it can be readjusted assembly station by proposing of particular disassembly equipment that supports the disassembly process. The paper deals with the analysis of
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Abdullah, Nurhidayu, Fairul Azni Jafar, and Mohd Nazmin Maslan. "Experimental Design for Reverse Assembly Based Disassembly Process." Applied Mechanics and Materials 660 (October 2014): 1062–66. http://dx.doi.org/10.4028/www.scientific.net/amm.660.1062.

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Product disassembly is a vital strategy for industrial recycling and remanufacturing which generates the desired parts and/or subassemblies by means of separation of a product into its elements. In order to increase disassembly capacity while maintaining the parts quality, new standards had to be established. Manual disassembly work perform by human will create inconsistency in the quality, efficiency, as well as time consuming, whereby it is believe that those weaknesses can be overcome by an automated performance. Therefore, this research work will re-evaluate the existing approach of disass
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11

Woodruff, Jeffrey B., David G. Drubin, and Georjana Barnes. "Spindle assembly requires complete disassembly of spindle remnants from the previous cell cycle." Molecular Biology of the Cell 23, no. 2 (2012): 258–67. http://dx.doi.org/10.1091/mbc.e11-08-0701.

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Incomplete mitotic spindle disassembly causes lethality in budding yeast. To determine why spindle disassembly is required for cell viability, we used live-cell microscopy to analyze a double mutant strain containing a conditional mutant and a deletion mutant compromised for the kinesin-8 and anaphase-promoting complex-driven spindle-disassembly pathways (td-kip3 and doc1Δ, respectively). Under nonpermissive conditions, spindles in td-kip3 doc1Δ cells could break apart but could not disassemble completely. These cells could exit mitosis and undergo cell division. However, the daughter cells co
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12

Soh, SL, S. K. Ong, and A. Y. C. Nee. "Design for assembly and disassembly for remanufacturing." Assembly Automation 36, no. 1 (2016): 12–24. http://dx.doi.org/10.1108/aa-05-2015-040.

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Purpose – This research aims to propose a methodology for a systematic, concurrent consideration of design for assembly (DFA) and disassembly guidelines and constraints for product remanufacturing. The methodology provides a holistic approach to design product from the remanufacturing perspective. Design/methodology/approach – The proposed methodology incorporates parts’ integration assessment and evaluation of part complexity and accessibility taking into consideration both DFA and design-for-disassembly (DFD) guidelines and constraints. Metrics for accessibility and complexity in retrieving
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13

Cerqueira, Carla, Yuk-Ying S. Pang, Patricia M. Day, et al. "A Cell-Free Assembly System for Generating Infectious Human Papillomavirus 16 Capsids Implicates a Size Discrimination Mechanism for Preferential Viral Genome Packaging." Journal of Virology 90, no. 2 (2015): 1096–107. http://dx.doi.org/10.1128/jvi.02497-15.

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ABSTRACTWe have established a cell-freein vitrosystem to study human papillomavirus type 16 (HPV16) assembly, a poorly understood process. L1/L2 capsomers, obtained from the disassembly of virus-like particles (VLPs), were incubated with nuclear extracts to provide access to the range of cellular proteins that would be available during assembly within the host cell. Incorporation of a reporter plasmid “pseudogenome” was dependent on the presence of both nuclear extract and ATP. Unexpectedly, L1/L2 VLPs that were not disassembled prior to incubation with a reassembly mixture containing nuclear
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14

Gwon, Youngdae, Brian A. Maxwell, Regina-Maria Kolaitis, Peipei Zhang, Hong Joo Kim, and J. Paul Taylor. "Ubiquitination of G3BP1 mediates stress granule disassembly in a context-specific manner." Science 372, no. 6549 (2021): eabf6548. http://dx.doi.org/10.1126/science.abf6548.

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Stress granules are dynamic, reversible condensates composed of RNA and protein that assemble in eukaryotic cells in response to a variety of stressors and are normally disassembled after stress is removed. The composition and assembly of stress granules is well understood, but little is known about the mechanisms that govern disassembly. Impaired disassembly has been implicated in some diseases including amyotrophic lateral sclerosis, frontotemporal dementia, and multisystem proteinopathy. Using cultured human cells, we found that stress granule disassembly was context-dependent: Specifically
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15

FORD, KEVIN J., and MARLA B. FELLER. "Assembly and disassembly of a retinal cholinergic network." Visual Neuroscience 29, no. 1 (2011): 61–71. http://dx.doi.org/10.1017/s0952523811000216.

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AbstractIn the few weeks prior to the onset of vision, the retina undergoes a dramatic transformation. Neurons migrate into position and target appropriate synaptic partners to assemble the circuits that mediate vision. During this period of development, the retina is not silent but rather assembles and disassembles a series of transient circuits that use distinct mechanisms to generate spontaneous correlated activity called retinal waves. During the first postnatal week, this transient circuit is comprised of reciprocal cholinergic connections between starburst amacrine cells. A few days befo
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16

Yan, Jie, Thomas J. Maresca, Dunja Skoko, et al. "Micromanipulation Studies of Chromatin Fibers in Xenopus Egg Extracts Reveal ATP-dependent Chromatin Assembly Dynamics." Molecular Biology of the Cell 18, no. 2 (2007): 464–74. http://dx.doi.org/10.1091/mbc.e06-09-0800.

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We have studied assembly of chromatin using Xenopus egg extracts and single DNA molecules held at constant tension by using magnetic tweezers. In the absence of ATP, interphase extracts were able to assemble chromatin against DNA tensions of up to 3.5 piconewtons (pN). We observed force-induced disassembly and opening–closing fluctuations, indicating our experiments were in mechanochemical equilibrium. Roughly 50-nm (150-base pair) lengthening events dominated force-driven disassembly, suggesting that the assembled fibers are chiefly composed of nucleosomes. The ATP-depleted reaction was able
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17

Emanuele, Michael J., Weijie Lan, Miri Jwa, Stephanie A. Miller, Clarence S. M. Chan, and P. Todd Stukenberg. "Aurora B kinase and protein phosphatase 1 have opposing roles in modulating kinetochore assembly." Journal of Cell Biology 181, no. 2 (2008): 241–54. http://dx.doi.org/10.1083/jcb.200710019.

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The outer kinetochore binds microtubules to control chromosome movement. Outer kinetochore assembly is restricted to mitosis, whereas the inner kinetochore remains tethered to centromeres throughout the cell cycle. The cues that regulate this transient assembly are unknown. We find that inhibition of Aurora B kinase significantly reduces outer kinetochore assembly in Xenopus laevis and human tissue culture cells, frog egg extracts, and budding yeast. In X. leavis M phase extracts, preassembled kinetochores disassemble after inhibiting Aurora B activity with either drugs or antibodies. Kinetoch
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18

Ding, Wan Jing. "Design Method of Reducer Oriented Maintenance." Advanced Materials Research 108-111 (May 2010): 1129–33. http://dx.doi.org/10.4028/www.scientific.net/amr.108-111.1129.

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Maintenance is an important part of the product lifecycle management. As important equipment widely used in machine industry, disassembly and maintenance of reducer is crucial. In this paper, according to position and assembly relationship of all parts of reducer in 3D and the basic principles of disassembly, a disassembly relationship matrix was defined. The matrix can well express the disassemblity of the every part in model. Then, a generation algorithm of disassembly sequence was proposed based on and operations. Finally disassembly sequence of reducer was generated in VB.
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19

Deepak, B. B. V. L., G. Bala Murali, and Sandeep Kumar Pandey. "Energy Efficient Disassembly Sequence Generation Using Subassembly Detection Method with Environmental and Economic Parts Selection." Journal of Advanced Manufacturing Systems 17, no. 03 (2018): 353–73. http://dx.doi.org/10.1142/s021968671850021x.

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Supply chains have been more serious for environmental issues for not only the reduction of CO2 emission but also for material starvation. To enhance a closed loop supply chain for assembled product, a disassembly system is required. At end-of-life, the assembly products at recycling organization should be disassembled environmentally and economically for not only low carbonization but also of material circulation. This recycling and reusing of product enables to reduce CO2 release because the virgin materials which release CO2 volumes caused by production and logistics can be saved by the usa
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Yi, Yang, Xiaojun Liu, Zhonghua Ni, Xia Tang, and Xiaokang Xu. "Research Method of Assembly Sequence Planning Based on Assembly Constraint Relationship Analysis." MATEC Web of Conferences 237 (2018): 03005. http://dx.doi.org/10.1051/matecconf/201823703005.

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In order to effectively reduce the human-computer interaction (HCI) workload before the assembly process simulation in the digital assembly environment, and to enhance the intelligence of assembly sequence planning, we introduce the assembly constraint relationship into the automatic generation of assembly sequences and propose a practical assembly sequence planning method for intelligent assembly. Firstly, starting from the assembly geometry model, an assembly model liaison graph is constructed by combining the analysis of assembly constraint relationship and the definition of disassembly dir
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McGovern, Seamus M., and Surendra M. Gupta. "Unified assembly- and disassembly-line model formulae." Journal of Manufacturing Technology Management 26, no. 2 (2015): 195–212. http://dx.doi.org/10.1108/jmtm-11-2013-0169.

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Purpose – There is a rich body of literature on sequencing assembly and on sequencing disassembly, but little that either fuses or contrasts the two, which may be valuable for long-range planning in the closed-loop supply chain and simply convenient in terms of consistency in nomenclature and mathematical formulations. The purpose of this paper is to concisely unify and summarize assembly and disassembly formulae – as well as to add new formulations for completeness – and then demonstrate the similarities and differences between assembly and disassembly. Design/methodology/approach – Along wit
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Filipescu, Adriana, Dan Ionescu, Adrian Filipescu, Eugenia Mincă, and Georgian Simion. "Multifunctional Technology of Flexible Manufacturing on a Mechatronics Line with IRM and CAS, Ready for Industry 4.0." Processes 9, no. 5 (2021): 864. http://dx.doi.org/10.3390/pr9050864.

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A communication and control architecture of a multifunctional technology for flexible manufacturing on an assembly, disassembly, and repair mechatronics line (A/D/RML), assisted by a complex autonomous system (CAS), is presented in the paper. A/D/RML consists of a six-work station (WS) mechatronics line (ML) connected to a flexible cell (FC) equipped with a six-degree of freedom (DOF) industrial robotic manipulator (IRM). The CAS has in its structure two driving wheels and one free wheel (2 DW/1 FW)-wheeled mobile robot (WMR) equipped with a 7-DOF robotic manipulator (RM). On the end effector
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Peiyong, Li, Cui Jin, Gao Feng, Wang Chengfang, Mao Yunsheng, and Liao Guohong. "Research on the Assembly Sequence of a Ship Block Based on the Disassembly Interference Matrix." Journal of Ship Production and Design 31, no. 04 (2015): 230–40. http://dx.doi.org/10.5957/jspd.2015.31.4.230.

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Reasonable optimized block assembly sequence has vital practical significance for ship construction, and the research has been done in this article at the aim of obtaining optimal assembly sequence. First, taking a double-bottom block as the object of study, by analyzing the connection relationship between each part, a disassembly network diagram and disassembly interference matrix can be generated by applying the demolition method. Based on the disassembly interference matrix, disassembly sequence can be generated according to certain rules, then the assembly sequence is the invert of disasse
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Kueh, Hao Yuan, Guillaume T. Charras, Timothy J. Mitchison, and William M. Brieher. "Actin disassembly by cofilin, coronin, and Aip1 occurs in bursts and is inhibited by barbed-end cappers." Journal of Cell Biology 182, no. 2 (2008): 341–53. http://dx.doi.org/10.1083/jcb.200801027.

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Turnover of actin filaments in cells requires rapid actin disassembly in a cytoplasmic environment that thermodynamically favors assembly because of high concentrations of polymerizable monomers. We here image the disassembly of single actin filaments by cofilin, coronin, and actin-interacting protein 1, a purified protein system that reconstitutes rapid, monomer-insensitive disassembly (Brieher, W.M., H.Y. Kueh, B.A. Ballif, and T.J. Mitchison. 2006. J. Cell Biol. 175:315–324). In this three-component system, filaments disassemble in abrupt bursts that initiate preferentially, but not exclusi
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Xiang, Dong, Zu Fu Pang, Dan Feng Long, Peng Mou, Ji Ping Yang, and Guang Hong Duan. "The Disassembly Process and Apparatus of Waste Printed Circuit Board Assembly for Reusing the Components." Applied Mechanics and Materials 457-458 (October 2013): 474–85. http://dx.doi.org/10.4028/www.scientific.net/amm.457-458.474.

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Printed Circuit Board Assembly (PCBA), is an important basic part of Electric and Electronic Equipment, which has lots of high-value components and toxic substances. Normally, when Electric and Electronic Equipment (EEE) is scraped, some components on waste PCBA still keep good performances. Therefore, it is feasible and economical to environment-friendly reuse the high-value components on waste PCBA. In order to guarantee the quality of the disassembled components, the components reuse-oriented disassembly process is proposed, in which the mechanical and physical characteristics of solder and
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Bono, Fulvia, and Niels H. Gehring. "Assembly, disassembly and recycling." RNA Biology 8, no. 1 (2011): 24–29. http://dx.doi.org/10.4161/rna.8.1.13618.

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Chuang, Edward, Acacia M. Hori, Christina D. Hesketh, and James Shorter. "Amyloid assembly and disassembly." Journal of Cell Science 131, no. 8 (2018): jcs189928. http://dx.doi.org/10.1242/jcs.189928.

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YAMAGIWA, Yasuyuki. "Design for Assembly Disassembly." Journal of the Japan Society for Precision Engineering 74, no. 8 (2008): 805–8. http://dx.doi.org/10.2493/jjspe.74.805.

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Sánchez, Irma, and Brian David Dynlacht. "Cilium assembly and disassembly." Nature Cell Biology 18, no. 7 (2016): 711–17. http://dx.doi.org/10.1038/ncb3370.

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Yokota, K., and D. R. Brough. "ASSEMBLY/DISASSEMBLY SEQUENCE PLANNING." Assembly Automation 12, no. 3 (1992): 31–38. http://dx.doi.org/10.1108/eb004372.

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Sun, Chengjun, Julien Arino, and Stéphanie Portet. "Intermediate filament dynamics: Disassembly regulation." International Journal of Biomathematics 10, no. 01 (2016): 1750015. http://dx.doi.org/10.1142/s1793524517500152.

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A mechanism of intermediate filament disassembly regulation is proposed in which disassembly is regulated by the amount of proteins assembled in networks. It is also hypothesized that a delay might exist between regulation and actual disassembly. Under realistic biological conditions of assembly and disassembly, it is shown that such a delay is harmless and does not destabilize the organization of intermediate filaments in networks. However, for high rates of disassembly, the model predicts that delay can destabilize the organization, with the intermediate filament material oscillating between
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Chen, Shiang-Fong, J. H. Oliver, Shuo-Yan Chou, and Lin-Lin Chen. "Parallel Disassembly by Onion Peeling." Journal of Mechanical Design 119, no. 2 (1997): 267–74. http://dx.doi.org/10.1115/1.2826246.

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For some assembly structures, parallel disassembly of components is necessary in order to reach a particular internal component. Due to the large number of possible combinations, the parallel disassembly problem is not easily solved in a general form. In order to reduce the time complexity of finding a disassembly sequence, this paper introduces a simplified mating graph and develops a data structure to facilitate an efficient parallel disassembly algorithm. This algorithm takes Max {O(N3), O(E)} time to find an efficient sequence to reach a particular component, where N is the number of compo
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Sung, Jinmo, and Bongju Jeong. "A Heuristic for Disassembly Planning in Remanufacturing System." Scientific World Journal 2014 (2014): 1–10. http://dx.doi.org/10.1155/2014/949527.

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This study aims to improve the efficiency of disassembly planning in remanufacturing environment. Even though disassembly processes are considered as the reverse of the corresponding assembly processes, under some technological and management constraints the feasible and efficient disassembly planning can be achieved by only well-designed algorithms. In this paper, we propose a heuristic for disassembly planning with the existence of disassembled part/subassembly demands. A mathematical model is formulated for solving this problem to determine the sequence and quantity of disassembly operation
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Nguyen Huy Liem, Le Anh, Pham Hoang Hung, and Mac Quynh Nhu. "Research and implementation of virtual reality technology to build 3D application that simulates the disassembly and assembly of equipment and weapons (DASim)." Journal of Military Science and Technology, CSCE7 (December 30, 2023): 61–70. http://dx.doi.org/10.54939/1859-1043.j.mst.csce7.2023.60-70.

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Disassembly and assembly of equipment and weapons (E&W) is a regular work of great importance in the military. Implementing the right procedures and quickly will help increase efficiency and ensure the safety for the citizens and weapons. However, the training and training of cadres, technical staff, trainees and soldiers to master the skills of disassembling equipment and weapons takes a lot of time and resources, and there are also many risks in the practice process. Virtual reality (VR) application is a time-saving and effective solution for training knowledge and training weapons disas
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Chromy, Laura R., Amy Oltman, Patricia A. Estes, and Robert L. Garcea. "Chaperone-Mediated In Vitro Disassembly of Polyoma- and Papillomaviruses." Journal of Virology 80, no. 10 (2006): 5086–91. http://dx.doi.org/10.1128/jvi.80.10.5086-5091.2006.

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ABSTRACT Hsp70 chaperones play a role in polyoma- and papillomavirus assembly, as evidenced by their interaction in vivo with polyomavirus capsid proteins at late times after virus infection and by their ability to assemble viral capsomeres into capsids in vitro. We studied whether Hsp70 chaperones might also participate in the uncoating reaction. In vivo, Hsp70 coimmunoprecipitated with polyomavirus virion VP1 at 3 h after infection of mouse cells. In vitro, prokaryotic and eukaryotic Hsp70 chaperones efficiently disassembled polyoma- and papillomavirus-like particles and virions in energy-de
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Bahubalendruni, M. V. A. Raju. "An efficient method for exploded view generation through assembly coherence data and precedence relations." World Journal of Engineering 15, no. 2 (2018): 248–53. http://dx.doi.org/10.1108/wje-06-2017-0126.

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Purpose Three-dimensional exploded view is a schematic representation of a product anticipated for performing assembly or disassembly operations. Exploded view is found in many applications, such as product instructional materials, repair and maintenance handbooks. This paper aims to propose an efficient exploded view generation technique based on assembly coherence data and disassembly feasibility testing, and illustrate it on various configurations of assemblies. Design/methodology/approach The proposed methodology extracts the assembly contact information between the constituent parts and g
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Warnock, Dale E., Takeshi Baba, and Sandra L. Schmid. "Ubiquitously Expressed Dynamin-II Has a Higher Intrinsic GTPase Activity and a Greater Propensity for Self-assembly Than Neuronal Dynamin-I." Molecular Biology of the Cell 8, no. 12 (1997): 2553–62. http://dx.doi.org/10.1091/mbc.8.12.2553.

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To begin to understand mechanistic differences in endocytosis in neurons and nonneuronal cells, we have compared the biochemical properties of the ubiquitously expressed dynamin-II isoform with those of neuron-specific dynamin-I. Like dynamin-I, dynamin-II is specifically localized to and highly concentrated in coated pits on the plasma membrane and can assemble in vitro into rings and helical arrays. As expected, the two closely related isoforms share a similar mechanism for GTP hydrolysis: both are stimulated in vitro by self-assembly and by interaction with microtubules or the SH3 domain-co
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Parra, Karlett J., Chun-Yuan Chan, and Jun Chen. "Saccharomyces cerevisiae Vacuolar H+-ATPase Regulation by Disassembly and Reassembly: One Structure and Multiple Signals." Eukaryotic Cell 13, no. 6 (2014): 706–14. http://dx.doi.org/10.1128/ec.00050-14.

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ABSTRACTVacuolar H+-ATPases (V-ATPases) are highly conserved ATP-driven proton pumps responsible for acidification of intracellular compartments. V-ATPase proton transport energizes secondary transport systems and is essential for lysosomal/vacuolar and endosomal functions. These dynamic molecular motors are composed of multiple subunits regulated in part by reversible disassembly, which reversibly inactivates them. Reversible disassembly is intertwined with glycolysis, the RAS/cyclic AMP (cAMP)/protein kinase A (PKA) pathway, and phosphoinositides, but the mechanisms involved are elusive. The
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Wang, Qian, Min Li Gong, and Ying Zhi Li. "Pro/E-Based Non-Damage Dynamic Simulation Assembling and Disassembling of CA6140." Applied Mechanics and Materials 184-185 (June 2012): 376–79. http://dx.doi.org/10.4028/www.scientific.net/amm.184-185.376.

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CA6140 is the most widely used kind of ordinary horizontal lathes in a factory workshop. This paper, with the aid of Pro / E software and its related functions, visually displays the structure of Lathe CA6140’s main components, dynamically simulates assembly and disassembly processes of its typical mechanism, and thereby realizes its non-damage dynamic simulation assembly and disassembly, creates the mode of combining actual and virtual dynamic assembly and disassembly and realizes the optimization of its assembly and disassembling actions.
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40

Zhao, Guan, and Yanfei Wang. "Development of Machine Tool Disassembly and Assembly Training and Digital Twin Model Building System based on Virtual Simulation." International Journal of Mechanical and Electrical Engineering 2, no. 1 (2024): 50–59. http://dx.doi.org/10.62051/ijmee.v2n1.07.

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In response to the issues of poor safety and insufficient training equipment in traditional machine tool assembly and disassembly training, this paper proposes a machine tool assembly and disassembly training system based on virtual simulation. The system utilizes Unity 3D to construct an interactive virtual training environment, achieving highly simulated simulations and teaching of the machine tool assembly and disassembly process. Additionally, the system is capable of establishing communication with actual machine tools, achieving bidirectional mapping between digital and physical spaces,
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41

Onischenko, Evgeny A., Natalia V. Gubanova, Elena V. Kiseleva, and Einar Hallberg. "Cdk1 and Okadaic Acid-sensitive Phosphatases Control Assembly of Nuclear Pore Complexes in Drosophila Embryos." Molecular Biology of the Cell 16, no. 11 (2005): 5152–62. http://dx.doi.org/10.1091/mbc.e05-07-0642.

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Disassembly and reassembly of the nuclear pore complexes (NPCs) is one of the major events during open mitosis in higher eukaryotes. However, how this process is controlled by the mitotic machinery is not clear. To investigate this we developed a novel in vivo model system based on syncytial Drosophila embryos. We microinjected different mitotic effectors into the embryonic cytoplasm and monitored the dynamics of disassembly/reassembly of NPCs in live embryos using fluorescently labeled wheat germ agglutinin (WGA) or in fixed embryos using electron microscopy and immunostaining techniques. We
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Chen, Ping, Caifu Qian, and Yunxiao Zhang. "Design and Stress Analysis of a New Quick-Opening Seal Device Connected by D-Shaped Shearing Bolts." Journal of Pressure Vessel Technology 129, no. 3 (2006): 550–55. http://dx.doi.org/10.1115/1.2757188.

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Because of large scale and heavy weight, end closures of high pressure vessels are usually hard to assemble and disassemble. In this paper, a new quick-opening seal device connected by D-shaped shearing bolts is introduced. This device is compact in structure, reliable in sealing, easy in assembly and disassembly, and very appropriate for end closures of pressure vessels or for pipe joints. Strength calculation formulas for the major parts are proposed analytically, and stress analysis using the finite element method for a C-ring seal has been performed. An application of this device shows tha
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Studny, D., D. Rittel, and E. Zussman. "Impact Fracture of Screws for Disassembly." Journal of Manufacturing Science and Engineering 121, no. 1 (1999): 118–26. http://dx.doi.org/10.1115/1.2830563.

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Environmental legislation urges manufacturers to develop effective technologies to cope with obsolete products. The goal is to optimize the disassembly procedure (cost, efficiency) with emphasis on potential recycling. In this work we adopt a destructive approach to product disassembly as an alternative to reverse assembly. Specifically we investigate unsupported screwed assemblies for which the screw head is protruding. Disassembly consist of breaking this head by applying side impact. The transient forces are measured by means of an instrumented bar. Experiments were conducted on various scr
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Liu, Lifeng, Eva Weiss, Marc D. Panas, et al. "RNA processing bodies are disassembled during Old World alphavirus infection." Journal of General Virology 100, no. 10 (2019): 1375–89. http://dx.doi.org/10.1099/jgv.0.001310.

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RNA processing bodies (P-bodies) are non-membranous cytoplasmic aggregates of mRNA and proteins involved in mRNA decay and translation repression. P-bodies actively respond to environmental stresses, associated with another type of RNA granules, known as stress granules (SGs). Alphaviruses were previously shown to block SG induction at late stages of infection, which is important for efficient viral growth. In this study, we found that P-bodies were disassembled or reduced in number very early in infection with Semliki Forest virus (SFV) or chikungunya virus (CHIKV) in a panel of cell lines. S
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Pan, Wanbin, Yigang Wang, and Peng Du. "Automatic disassembly navigation for accurate virtual assembly path planning." Assembly Automation 34, no. 3 (2014): 244–54. http://dx.doi.org/10.1108/aa-01-2014-008.

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Purpose – The purpose of this paper is to develop an automatic disassembly navigation approach for human interactions in the virtual environment to achieve accurate and effective virtual assembly path planning (VAPP). Design/methodology/approach – First, to avoid the error-prone human interactions, a constraint-based disassembly method is presented. Second, to automatically provide the next operable part(s), a disassembly navigation mechanism is adopted. Finally, the accurate assembly path planning can be obtained effectively and automatically by inversing the ordered accurate disassembly path
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Srinivasan, Hari, and Rajit Gadh. "Efficient Geometric Disassembly of Multiple Components from an Assembly Using Wave Propagation." Journal of Mechanical Design 122, no. 2 (2000): 179–84. http://dx.doi.org/10.1115/1.533567.

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This paper analyzes the problem of disassembling multiple selected components from an assembly, defined as selective disassembly, and presents algorithms for efficient disassembly analysis of geometric models. Applications for selective disassembly include assembling, maintenance and recycling. A new approach called ‘Disassembly Wave Propagation’ is proposed to determine a selective disassembly sequence with minimal component removals from an assembly. This approach defines: (i) disassembly waves to topologically arrange the components denoting the disassembly order and (ii) intersection event
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Endo, Masayuki. "Surface Assembly of DNA Origami on A Lipid Bilayer Observed Using High-Speed Atomic Force Microscopy." Molecules 27, no. 13 (2022): 4224. http://dx.doi.org/10.3390/molecules27134224.

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The micrometer-scale assembly of various DNA nanostructures is one of the major challenges for further progress in DNA nanotechnology. Programmed patterns of 1D and 2D DNA origami assembly using specific DNA strands and micrometer-sized lattice assembly using cross-shaped DNA origami were performed on a lipid bilayer surface. During the diffusion of DNA origami on the membrane surface, the formation of lattices and their rearrangement in real-time were observed using high-speed atomic force microscopy (HS-AFM). The formed lattices were used to further assemble DNA origami tiles into their cavi
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Gutiérrez, T., J. I. Barbero, and A. Eguidazu. "Virtual Assembly and Disassembly Simulation." IFAC Proceedings Volumes 31, no. 7 (1998): 35–40. http://dx.doi.org/10.1016/s1474-6670(17)40253-9.

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Boothroyd, G., and L. Alting. "Design for Assembly and Disassembly." CIRP Annals 41, no. 2 (1992): 625–36. http://dx.doi.org/10.1016/s0007-8506(07)63249-1.

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Heride, Claire, Sylvie Urbé, and Michael J. Clague. "Ubiquitin code assembly and disassembly." Current Biology 24, no. 6 (2014): R215—R220. http://dx.doi.org/10.1016/j.cub.2014.02.002.

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