Thèses sur le sujet « Atg18 »
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Stephan, Joseph. « An Evolutionary Proteomics Approach For The Identification Of Pka Targets In Saccharomyces Cerevisiae Identifies Atg1 And Atg13, Two Proteins That Play A Central Role In The Regulation Of Autophagy By The Ras/Pka Pathway And The Tor Pathway ». The Ohio State University, 2008. http://rave.ohiolink.edu/etdc/view?acc_num=osu1218042573.
Texte intégralJenzer, Céline. « Physiopathologie de l’autophagie au cours du développement embryonnaire chez Caenorhabditis elegans ». Thesis, Université Paris-Saclay (ComUE), 2016. http://www.theses.fr/2016SACLS201.
Texte intégralMacroautophagy is a major ubiquitous catabolic process which allows the bulk degradation and recycling of cytoplasmic constituents by formation of double membrane vesicles called autophagosomes which then fuse with lysosomes. This process is involved in a large variety of physiological processes such as development, anti-aging, cell death and in human pathologies like cancers or neurodegenerative diseases. My thesis work revealed the existence of sequential and specific roles of autophagic proteins LGG-1 and LGG-2, homologs of Atg8/LC3 in Caenorhabditis elegans. In this study, we focused on a particular population of autophagosomes involved in a physiological process in early embryos: the degradation of paternal mitochondria during fertilization. We showed that LGG-1 is recruited at the early autophagosomes and allows LGG -2 recruitment which acts later in the autophagic process to allow the fusion of autophagosomes with lysosomes. Moreover, the function of LGG -1 can be complemented with its human homologs revealing the interest of the C. elegans model system for analyzing Atg8 homologs.Furthermore, recent studies have identified the recruitment of autophagic proteins during phagocytosis of apoptotic cells in the so called LC3-associated phagocytosis (LAP). By genetic and cellular approaches, using optical and electron microscopy, I showed that there is a different involvement of autophagic proteins, LGG-1 and LGG-2 in the degradation of apoptotic cells in C. elegans. LGG-2 protein, specifically, plays a role in phagocytic cell to degrade apoptotic corpses. Moreover, this work suggest a function of autophagy in the apoptotic corpses to allow phagocytosis
[Verfasser], Archna, et Michael [Akademischer Betreuer] Steinert. « Role of ATG12-ATG5 conjugate in autophagy regulation / Archna ; Betreuer : Michael Steinert ». Braunschweig : Technische Universität Braunschweig, 2017. http://d-nb.info/1175817538/34.
Texte intégralKhaliq, Samira. « Characterization of Atg18p and its role in cellular trafficking in Saccharomyces cerevisiae ». Thesis, University of Birmingham, 2013. http://etheses.bham.ac.uk//id/eprint/4010/.
Texte intégralLeveque, Maude. « Elucidating the canonical and non-canonical functions of the autophagy protein TgATG8 in the apicomplexan parasite Toxoplasma gondii ». Thesis, Montpellier, 2016. http://www.theses.fr/2016MONTT031.
Texte intégralAutophagy is a self-degradative process evolutionary conserved among eukaryotes. Typically induced by starvation, it involves the formation of a double membrane compartment called the autophagosome to sequester and deliver intracellular components for lysosomal degradation and recycling. The protein ATG8 occupies a central position in this process and is recruited to autophagosomal membranes by a highly regulated conjugation system. Toxoplasma gondii is a parasitic protist belonging to the Apicomplexa phylum, which possesses a reduced autophagy machinery. This obligate intracellular parasite is nevertheless able to generate TgATG8-decorated autophagosomes upon nutrient stress. Surprisingly, during normal intracellular parasite growth, TgATG8 mainly localizes to the apicoplast, a non-photosynthetic plastid acquired by secondary endosymbiosis which hosts essential metabolic pathways. My thesis aimed to elucidate the canonical and non-canonical roles of ATG8 in Toxoplasma. The first part of this study is the functional and spatio-temporal characterization of TgATG8 association with the apicoplast. We showed TgATG8 is recruited to both ends of the elongating plastid during parasite division, and allows the maintenance of the organelle across generations by permitting its centrosome-driven distribution into the two daughter cells. The second part of this work is the isolation and mass spectrometry-based identification of putative TgATG8-interacting proteins that would be involved in autophagy-related or non-canonical functions. We analyzed the subcellular localization of nine candidates and functional studies were conducted for three proteins. Although we were unable to confirm their interactions with TgATG8, this approach allowed the identification of novel and important parasite proteins: an essential apicoplast phospholipase, a potential regulator of the cell cycle, and a component of the parasite cytoskeleton
Chew, Leon Harold. « Structural characterization of the Atg1 kinase complex by single particle electron microscopy ». Thesis, University of British Columbia, 2013. http://hdl.handle.net/2429/45666.
Texte intégralMayrhofer, Peter [Verfasser], et Thomas [Akademischer Betreuer] Wollert. « Atg11 initiates selective autophagy in yeast by tethering Atg9 vesicles / Peter Mayrhofer ; Betreuer : Thomas Wollert ». München : Universitätsbibliothek der Ludwig-Maximilians-Universität, 2019. http://d-nb.info/1209472384/34.
Texte intégralYeh, Yuh-Ying. « The regulation of Atg1 protein kinase activity is important to the autophagy process in Saccharomyces cerevisiae ». The Ohio State University, 2010. http://rave.ohiolink.edu/etdc/view?acc_num=osu1290439442.
Texte intégralMatscheko, Nena Magdalena [Verfasser], et Stefan [Akademischer Betreuer] Jentsch. « Revealing the molecular mechanism of Atg11 and the initation of selective autophagy / Nena Magdalena Matscheko ; Betreuer : Stefan Jentsch ». München : Universitätsbibliothek der Ludwig-Maximilians-Universität, 2016. http://d-nb.info/1171705336/34.
Texte intégralSanwald, Julia [Verfasser], Dieter [Gutachter] Willbold et Björn [Gutachter] Stork. « The ATG8 Protein GABARAP in Secretion, Transport, and Autophagy / Julia Sanwald ; Gutachter : Dieter Willbold, Björn Stork ». Düsseldorf : Universitäts- und Landesbibliothek der Heinrich-Heine-Universität Düsseldorf, 2021. http://d-nb.info/1225146569/34.
Texte intégralMunzel, Lena [Verfasser], Michael [Akademischer Betreuer] Thumm, Michael [Gutachter] Thumm et Gerhard [Gutachter] Braus. « Atg21 restricts Atg8 lipidation to a novel vacuole-phagophore contact site / Lena Munzel ; Gutachter : Michael Thumm, Gerhard Braus ; Betreuer : Michael Thumm ». Göttingen : Niedersächsische Staats- und Universitätsbibliothek Göttingen, 2019. http://d-nb.info/1202604927/34.
Texte intégralBudovskaya, Yelena V. « An Evaluationary Proteomics Approach for the Identification of Substrates of the Camp-Dependent Protein Kinase in Saccharomyces Cerevisiae ». Connect to this title online, 2005. http://rave.ohiolink.edu/etdc/view?acc%5Fnum=osu1104152442.
Texte intégralTitle from first page of PDF file. Document formatted into pages; contains xiv, 128 p.; also includes graphics (some col.) Includes bibliographical references (p. 117-132).
Hofmann, Benjamin [Verfasser], Günther [Akademischer Betreuer] [Gutachter] Woehlke et Friedrich C. [Gutachter] Simmel. « Spatial Coordination of Atg8-Lipidation in Selective and Non-selective Autophagy / Benjamin Hofmann ; Gutachter : Günther Woehlke, Friedrich C. Simmel ; Betreuer : Günther Woehlke ». München : Universitätsbibliothek der TU München, 2016. http://d-nb.info/1123729158/34.
Texte intégral三河, 拓己. « 分裂酵母Vps1とAtg8の酸化ストレス抵抗性における機能の解析 ». 京都大学 (Kyoto University), 2010. http://hdl.handle.net/2433/120351.
Texte intégralSeegobin, Matthew. « Examining Parkinson’s Disease Linked DJ-1 and its Interaction with Autophagy Related ATG5 and ATG12 & ; Understanding PINK1’s Functional Interaction with Mitochondrial m-AAA Protease AFG3L2 ». Thesis, Université d'Ottawa / University of Ottawa, 2017. http://hdl.handle.net/10393/35701.
Texte intégralPerna, Marco [Verfasser], Johannes [Akademischer Betreuer] Buchner et Sevil [Akademischer Betreuer] Weinkauf. « In vitro reconstitution of the Atg1-kinase complex : Revealing the molecular mechanism of autophagy initiation / Marco Perna. Gutachter : Sevil Weinkauf ; Johannes Buchner. Betreuer : Johannes Buchner ». München : Universitätsbibliothek der TU München, 2014. http://d-nb.info/1059857049/34.
Texte intégralWalker, Dawn Marie. « The Study of Autophagy in Plasmodium falciparum ». The Ohio State University, 2013. http://rave.ohiolink.edu/etdc/view?acc_num=osu1385586661.
Texte intégralVogt, Benjamin [Verfasser], Kay [Akademischer Betreuer] Hofmann et R. Jürgen [Akademischer Betreuer] Dohmen. « A bioinformatical approach for a reliable determination of short motifs for SUMO and Atg8 interaction in Saccharomyces cerevisiae / Benjamin Vogt. Gutachter : Kay Hofmann ; R. Jürgen Dohmen ». Köln : Universitäts- und Stadtbibliothek Köln, 2014. http://d-nb.info/1051077400/34.
Texte intégralMitter, Anne Lisa [Verfasser], Michael [Akademischer Betreuer] Thumm, Silvio [Gutachter] Rizzoli et Stefanie [Gutachter] Pöggeler. « Dissecting the molecular function of the ubiquitin-like Atg8 during autophagosome biogenesis in S. cerevisiae / Anne Lisa Mitter ; Gutachter : Silvio Rizzoli, Stefanie Pöggeler ; Betreuer : Michael Thumm ». Göttingen : Niedersächsische Staats- und Universitätsbibliothek Göttingen, 2018. http://d-nb.info/1180026357/34.
Texte intégralJuris, Lisa Angelika [Verfasser], Michael [Akademischer Betreuer] Thumm, Volker [Akademischer Betreuer] Lipka, Karin [Akademischer Betreuer] Kühnel, Stefanie [Akademischer Betreuer] Pöggeler, Detlef [Akademischer Betreuer] Doenecke et Dieter [Akademischer Betreuer] Schmitt. « Atg21 functions during autophagy as a scaffold for the E3 ubiquitin-‐like complex in Atg8 lipidation / Lisa Angelika Juris. Gutachter : Michael Thumm ; Volker Lipka ; Karin Kühnel ; Stefanie Pöggeler ; Detlef Doenecke ; Dieter Schmitt. Betreuer : Michael Thumm ». Göttingen : Niedersächsische Staats- und Universitätsbibliothek Göttingen, 2015. http://d-nb.info/1065882351/34.
Texte intégralJuris, Lisa Angelika Verfasser], Michael [Akademischer Betreuer] Thumm, Volker [Akademischer Betreuer] Lipka, Karin [Akademischer Betreuer] Kühnel, Stefanie [Akademischer Betreuer] Pöggeler, Detlef [Akademischer Betreuer] [Doenecke et Dieter [Akademischer Betreuer] Schmitt. « Atg21 functions during autophagy as a scaffold for the E3 ubiquitin-‐like complex in Atg8 lipidation / Lisa Angelika Juris. Gutachter : Michael Thumm ; Volker Lipka ; Karin Kühnel ; Stefanie Pöggeler ; Detlef Doenecke ; Dieter Schmitt. Betreuer : Michael Thumm ». Göttingen : Niedersächsische Staats- und Universitätsbibliothek Göttingen, 2015. http://d-nb.info/1065882351/34.
Texte intégralBusse, Ricarda. « Insights into membrane binding of PROPPINs and Reconstitution of mammalian autophagic conjugation systems ». Doctoral thesis, 2013. http://hdl.handle.net/11858/00-1735-0000-0001-B95B-B.
Texte intégralScacioc, Andreea. « Structural, biochemical and computational studies on PROPPINs, proteins important in autophagy ». Doctoral thesis, 2014. http://hdl.handle.net/11858/00-1735-0000-0028-8675-3.
Texte intégralSuttangkakul, Anongpat. « Characterization of ATG1 regulatory complex in Arabidopsis thaliana ». 2008. http://www.library.wisc.edu/databases/connect/dissertations.html.
Texte intégralSchalk, Amanda Marie. « Structural and functional characterization of the autophagy proteins Atg5 and Atg16L1 and their interaction partners ». Doctoral thesis, 2011. http://hdl.handle.net/11858/00-1735-0000-0006-AE0A-0.
Texte intégralHo, Kung-Hsien, et 何恭憲. « Characterization of different functional sites of Atg8 in Saccharomyces cerevisiae ». Thesis, 2007. http://ndltd.ncl.edu.tw/handle/96863953850893804690.
Texte intégral國立臺灣大學
動物學研究研究所
95
Autophagy is a highly conserved membrane trafficking pathway, which is evoked during stress condition, such as nutrient starvation. Excess or abnormal intracellular macromolecules are sequestered by the double-membrane vesicle, autophagosome, and transported to the vacuole/lysosome for degradation and recycling of nutrient. The released amino acids are used in synthesis of proteins required for cells to adapt to the changed environment. Atg8 is an essential regulator for autophagosome biogenesis. It is post-translationally conjugated to lipid at its C-terminus and is proposed to be the membrane modifier that may be functionally similar to coat proteins. Besides, Atg8 also interacts directly with the autophagic cargo receptor, Atg19. We are interested in whether the Atg8-mediated vesicle expansion process and cargo sorting are coupled. Here we identified 6 residues on Atg8 surface that are required for the modification and/or physiological function of it. Residue Arg28 is specific for the cargo receptor binding; Tyr49, Leu50, Leu55, and Phe79 are involved in different steps of its post-translational modification; and Lys48 is important for perhaps the biogenesis of autophagosome.
Ho, Kung-Hsien. « Characterization of different functional sites of Atg8 in Saccharomyces cerevisiae ». 2007. http://www.cetd.com.tw/ec/thesisdetail.aspx?etdun=U0001-2507200716025600.
Texte intégralTang, Hong-Wen, et 唐弘文. « Induction of autophagy and apoptosis by Atg1 to promote cell death ». Thesis, 2007. http://ndltd.ncl.edu.tw/handle/39694543867412379166.
Texte intégral臺灣大學
生化科學研究所
95
Atg1 encodes a Serine/Threonine kinase. In yeast, studies have found the requirement for Atg1 kinase activity in both CVT and autophagy. Atg1 interacts with multiple components of the autophagic machinery, and multiple signaling pathways converge on Atg1 to regulate autophagy. Thus, Atg1 is likely to represent a nodal point for controlling multiple steps in the autophagic process in response to various stresses. However, in higher eukaryotes, the role of Atg1 is still unclear. It has been shown that autophagy can induce cells death. However, the molecular mechanism underlying the autophagic cell death program is unclear. I have examined a potential role for Atg1 in cell death using Drosophila melanogaster as a model system. My results demonstrate that dAtg1 is sufficient to cause cell death due to the induction of autophagy and apoptosis. These findings provide a direct evidence that autophagy and apoptosis are interconnected. I have also identified a novel protein, dDlk. My results demonstrated that dDlk can induce cell death. The biochemical and genetic data reveal that dDlk interacts with dAtg1 and functions downstream of dAtg1-mediated cell death. Thus, dDlk may be an important downstream player in mediating dAtg1’s biological effects on promoting cell death. I am currently investigating whether dDlk is mediated by Atg1 and what is the biological function of Atg1 in cell death.
Chuang, Li-Jin, et 莊麗瑾. « A Screen for modifiers of Atg1-mediated signaling in Drosophila development ». Thesis, 2010. http://ndltd.ncl.edu.tw/handle/35138851153829891550.
Texte intégral臺灣大學
生化科學研究所
98
Abstracts Autophagy is a highly conserved cellular process that involves vesicle-mediated sequestration and degradation of cytoplasmic proteins and organelles. Atg1 is a Ser/Thr kinase that is regulated by TOR-dependent signaling. In yeast, studies have found the requirement for Atg1 kinase activity in both CVT and autophagy. Thus, Atg1 is representing a nodal point for controlling multiple steps in autophagic process in response to various stresses. I have examined that overexpression of Drosophila Atg1 in the developing compound eye triggers cell death and results in eye roughness. Although a number of proteins have been found to associate with Atg1, the identification of Atg1 substrates important for autophagy remains a difficult task. To identify novel genes involved in the Atg1-mediated pathway, I carried out a dominant modifier screen of the Atg1-induced rough eye phenotype using contiguous chromosomal deficiencies that represent more than 70% of the Drosophila genome. Of the 277 deficiencies tested, 26 were identified as suppressors of Atg1 signaling. I characterize a subset of autosomal regions that strongly interact with Atg1. Three novel genes will likely identify Atg1 regulators and should shed some light on how cells are regulated by the balance between cell survival and cell death. I am currently investigating whether these regulators have physical interaction with Atg1 and what is the biological function in cell death.
Tseng, Ben-Lyu, et 曾本嵂. « Study on the Role of atg8 and Autophagy in Planarian Regeneration ». Thesis, 2012. http://ndltd.ncl.edu.tw/handle/26054051918736145532.
Texte intégral國立臺灣大學
動物學研究所
100
Autophagy mediates the bulk turnover of cytoplasmic constituents in lysosomes, an important process for cellular metabolism. Autophagy can be induced by various stress stimuli, e.g., nutrient depletion or hypoxic stress. Upon autophagy activation, Atg8 is one of the autophagic proteins that mediate this pathway. Lipidation of Atg8 is used as a marker to monitor autophagy activity. Planarian, due to its high regenerative ability, has been used as the animal model with which to study regeneration. During regeneration, both apoptosis and cell proliferation are evoked in planarian and the separated worm is regenerated into a well-proportioned small planarian. In order to verify if autophagy participates in planarian regeneration, we cloned the atg8 homolog (Djatg8) from Dugesia japonica, which is the only available species of planarian in Taiwan. Sequence analysis of Djatg8 indicated that it highly conserves with human GABARAP (GABA receptor associated protein). Whole mount in situ hybridization of Djatg8 showed that it is expressed throughout the whole body, including pharynx and neuron system. Expression of YFP-tagged Djatg8 in HeLa cells revealed its subcellular localization at autophagosomes/autolysosmes. We also show the functional conservation of Djatg8 in yeast autophagy pathway. By detecting the lipidation of Djatg8, we found that irradiation induces autophage pathway, while there was no significant up-regulation of autophagy during regeneration or starvation process. However, RNAi of Djatg8 does not affect the regeneration of planarian. Our results suggested that autophagy is not the major anabolic process in planarian reorganization, while the ubiquitous expression of Djatg8 implied that autophagy may be involved in other critical functions of planarian.
Tsau, Ming-Tong, et 曹明通. « Cloning and analysis of autophagy-related protein 8 (ATG8) in shrimp ». Thesis, 2013. http://ndltd.ncl.edu.tw/handle/64770529536616165081.
Texte intégralTang, Hong-Wen. « Induction of autophagy and apoptosis by Atg1 to promote cell death ». 2007. http://www.cetd.com.tw/ec/thesisdetail.aspx?etdun=U0001-2507200723562000.
Texte intégralMunzel, Lena. « Atg21 restricts Atg8 lipidation to a novel vacuole-phagophore contact site ». Doctoral thesis, 2019. http://hdl.handle.net/21.11130/00-1735-0000-0003-C14A-0.
Texte intégralVarberg, Joseph M. « Biochemical and pharmacological characterization of the Atg8 conjugation system in toxoplasma gondii ». Diss., 2017. http://hdl.handle.net/1805/14602.
Texte intégralToxoplasma gondii is an important human pathogen that infects millions of people worldwide and causing severe and potentially lethal disease in immunocompromised individuals. Recently, a homologue for the autophagy protein Atg8 (TgAtg8) was identified in Toxoplasma that is required for both canonical and noncanonical processes essential for parasite viability. Importantly, TgAtg8 functionality requires its conjugation to phosphatidylethanolamine through the activity of the Atg8 conjugation system. In this thesis, we characterized the proteins that interact with TgAtg8 and TgAtg3, a component of the Atg8 conjugation system, to further define their functions in Toxoplasma and identify opportunities for targeted inhibition of Atg8-related processes. We previously identified that TgAtg8 is acetylated at lysine 23 (K23) and assessed the role of this modification in this thesis. Using mutagenesis, we showed that K23 acetylation did not modulate the interaction with TgAtg3, but appeared to promote TgAtg8 protein stability. Additionally, endogenous mutation of K23 to the nonacetylatable amino acid arginine resulted in severe impairment of parasite replication and spontaneous differentiation into bradyzoites. To gain insight into the role of TgAtg8 in Toxoplasma biology, we next characterized TgAtg8 and TgAtg3 interacting proteins using affinity purification and mass spectrometry. We identified a novel group of interacting proteins that are unique to Toxoplasma, including the dynamin-related protein DrpC. Functional characterization of DrpC identified a potential role of TgAtg8 in trafficking of membrane from the Golgi to the nascent daughter parasites during replication. Lastly, we examined a group of small molecules recently identified as Atg3-Atg8 inhibitors in Plasmodium falciparum and assessed their activity against Toxoplasma. Although the compounds effectively inhibited Toxoplasma replication, they did so through novel mechanisms of action unrelated to the disruption of the TgAtg3-Atg8 interaction. Together, this work provides insight into the function of the Atg8 conjugation system in Toxoplasma that will help guide the future development of novel therapeutics targeting Atg8-related processes.
Wu, Jui-Hsuan, et 吳睿軒. « Characterization of function of MLF, ATG8 and FYVE containing protein in Giardia lamblia ». Thesis, 2017. http://ndltd.ncl.edu.tw/handle/z5fcks.
Texte intégral國立臺灣大學
微生物學研究所
105
Giardia lamblia is an early-branching and widely distributed intestinal protozoan parasites. People get infected by drinking the contaminated water. G. lamblia have two stages in the life cycle: a binucleate trophozoite and a quadrinucleate cyst. G. lamblia trophozoite parasitizies in small intestine and encysts by PH value changes. Autophagy is a self-degradative process of organisms that is an important balance of cell cycle in development and metabolism. In the previous reference, ATG8 was associated with the autophagosomes formation in Saccharomyces cerevisiae, and can be used as markers of autophagy. FYVE protein promotes autophagosomes formation in human and interacts with human autophagy-related protein-LC3 .We found localization of myeloid leukemia factor (MLF) in the vesicles. We’ve discovered autophagosome maker protein LC3/ATG8 related protein and FYVE protein that may transport vesicles in the Giardia by search genome database. This research will determine G. lamblia autophagy related mechanism. We observed ATG8 and FYVE proteins both located in MLF vesicles. MLF over expression can induce CWP1 protein level. In earlier experiments, we found the G. lamblia cell cycle relate CDK2 protein is cytosolic, but CDK2dm with a deletion of 49-1742a.a localized in MLF contained vesicles. To test the characteristics of these vesicles, we performed the starvation analysis, and treated PBS to cause starvation response in Giardia wild type WB strain. We found increased levels of MLF protein expression. We tested various autophagy inhibitors. Chloroquine inhibits the fusion of autophagosomes with lysosomes, and causes increased autophagosome formation. Nocodazole causes lysosomal damage, and decreases fusion ability of autophagosomes with lysosomes, resulting in an increase in autophagosome formation. MG132 is a proteasome inhibitor, that increases damage proteins and autophagy formation. Wortmannin is phagophore inhibitor and can inhibit autophagy formation. Dithiothreitol (DTT) can interfere the folding of proteins in ER, and induce expression of autophagosme maker protein-LC3. Puromycin (PU) and G418 will cause Giardia death. E. coli can induce autophagy (Xenophagy) in mammal cell, and can be used to test Xenophagy in Giardia. First, we treated chloroquine, nocodazole, MG132, wortmannin, DTT, G418, puromycin (PU) , and E. coli in the Giardia wild type WB cells. We found increased numbers of MLF vesicles and increased MLF protein expression by chloroquine, nocodazole, MG132, wortmannin, DTT, G418, puromycin and E. coli treatment. The expression of BIP protein was also increased in the chloroquine treatment experimental group. Then we tested the starvation effect in pATG8 experession strain. Increased numbers of ATG8 vesicles and increased ATG8 protein expression was found. We treated chloroquine, nocodazole, MG132, DTT, G418, and E. coli in the pATG8 expression strain, and found increased numbers of ATG8 vesicles and increased ATG8 protein expression. Using immunoprecipitation methods, we found that ATG8 proteins interacted with BIP. We transfected human LC3b gene in Giardia and created Giardia’s human LC3b expression strain-pLC3b. We found pLC3b interacted with G. lamblia MLF protein. The results showed that human LC3b was similar to G. lamblia ATG8 protein. Next we tested the starvation effect in pFYVE experession strain. Increased numbers of FYVE vesicles and increased FYVE protein expression was found. We treated chloroquine, nocodazole, MG132, DTT, G418, and E. coli in the pFYVE expression strain, and found increased numbers of FYVE vesicles and increased FYVE protein expression. We created Giardia’s mutation strain-pFYVEm1, and found that pFYVEml expression strain, expressed lower level of CWP1 protein than pFYVE expression strain. Using immunoprecipitation methods, we found that FYVE proteins interacted with BIP, and FYVE protein also interacted with ATG8 protein. MLF protein may be an autophagy related protein in Giardia. We also wanted to know the function of human MLF2 protein in Giardia. We transfected human MLF2 gene in Giardia and created Giardia’s human MLF2 expression strain-phMLF2. We tested the starvation effect in phMLF2 experession strain. Increased numbers of hMLF2 and increased hMLF2 protein expression was found. We treated chloroquine, nocodazole, MG132, DTT, G418, and E. coli in the phMLF2 expression strain, and found increased numbers of hMLF2 vesicles and increased hMLF2 protein expression. Using immunoprecipitation methods, we found hMLF2 proteins interacted with G. lamblia MLF protein. We treat chloroquine in the pCDK2dm expression strain, and found increased numbers of CDK2dm vesicles and increased CDK2dm protein expression. Next, we transfected pMLFHA plasmid in the CDK2dm expression strain. And found increased level of MLF and CDK2dm proteins and number of those vesicles, but reduced CWP1 protein expression. We provide evidence that the MLF、ATG8 and FYVE protein expression increased and numbers of vesicles by autophagy inhibitors trement. Using a mutant protein model CDK2dm, we also know that vesicles may transport mutant protein. Key words: Giardia lamblia, Autophagy, ATG8, FYVE domain, MLF, Chloroquine
Mitter, Anne Lisa. « Dissecting the molecular function of the ubiquitin-like Atg8 during autophagosome biogenesis in S. cerevisiae ». Doctoral thesis, 2018. http://hdl.handle.net/11858/00-1735-0000-002E-E39E-9.
Texte intégralFineis, Peter. « Autophagic regulation of Wolbachia in Drosophila ». Thesis, 2019. https://hdl.handle.net/2144/41522.
Texte intégral2021-10-09T00:00:00Z
Juris, Lisa Angelika. « Atg21 functions during autophagy as a scaffold for the E3 ubiquitin-‐like complex in Atg8 lipidation ». Doctoral thesis, 2014. http://hdl.handle.net/11858/00-1735-0000-0022-5D90-4.
Texte intégralSemerádová, Hana. « Vazba paralogů EXO70 na ATG8 a funkční rozdělení rodiny EXO70 dle účasti v autofagii (Arabidopsis thaliana) ». Master's thesis, 2015. http://www.nusl.cz/ntk/nusl-343788.
Texte intégralWerner, Antonia. « The interplay of SmNBR1 and SmATG8 in selective autophagy of the filamentous fungus Sordaria macrospora ». Doctoral thesis, 2017. http://hdl.handle.net/11858/00-1735-0000-002E-E38F-B.
Texte intégralWelter, Evelyn. « Identification of novel components involved in selective and unselective autophagic pathways ». Doctoral thesis, 2011. http://hdl.handle.net/11858/00-1735-0000-000D-F0AE-7.
Texte intégralMontino, Marco. « Dissecting molecular elements of mitophagy and the lysis of intravacuolar vesicles ». Doctoral thesis, 2015. http://hdl.handle.net/11858/00-1735-0000-0023-965C-8.
Texte intégralOtto, Florian Bo. « Dissection of the molecular machinery of micro- and macronucleophagy ». Doctoral thesis, 2019. http://hdl.handle.net/21.11130/00-1735-0000-0005-1297-C.
Texte intégral