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Articles de revues sur le sujet « Bacteriophages Genetics »

Auteur : Grafiati

Publié le 4 juin 2021

Mis à jour le 28 janvier 2023

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1

Auslander, Noam, Ayal B. Gussow, Sean Benler, Yuri I. Wolf, and Eugene V. Koonin. "Seeker: alignment-free identification of bacteriophage genomes by deep learning." Nucleic Acids Research 48, no. 21 (October 12, 2020): e121-e121. http://dx.doi.org/10.1093/nar/gkaa856.

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Abstract Recent advances in metagenomic sequencing have enabled discovery of diverse, distinct microbes and viruses. Bacteriophages, the most abundant biological entity on Earth, evolve rapidly, and therefore, detection of unknown bacteriophages in sequence datasets is a challenge. Most of the existing detection methods rely on sequence similarity to known bacteriophage sequences, impeding the identification and characterization of distinct, highly divergent bacteriophage families. Here we present Seeker, a deep-learning tool for alignment-free identification of phage sequences. Seeker allows

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Timmons, Michael S., M. Lieb, and Richard C. Deonier. "RECOMBINATION BETWEEN IS5 ELEMENTS: REQUIREMENT FOR HOMOLOGY AND RECOMBINATION FUNCTIONS." Genetics 113, no. 4 (August 1, 1986): 797–810. http://dx.doi.org/10.1093/genetics/113.4.797.

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ABSTRACT Intermolecular recombination between two IS5 elements was measured, using bacteriophage lambda recombination vectors, and was compared to recombination between two copies of an SV40 segment cloned into the same vectors. Experiments were conducted in the presence and in the absence of RecA and Red functions, and with the recombining inserts in the same or in reversed orientation. Under all conditions, IS5 elements recombined in a manner similar to the SV40 inserts, indicating that IS-encoded functions did not confer measurable additional intermolecular recombination ability to IS5 in E

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Węgrzyn, Grzegorz. "Should Bacteriophages Be Classified as Parasites or Predators?" Polish Journal of Microbiology 71, no. 1 (February 23, 2022): 3–9. http://dx.doi.org/10.33073/pjm-2022-005.

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Abstract Bacteriophages are viruses infecting bacteria and propagating in bacterial cells. They were discovered over 100 years ago, and for decades they played crucial roles as models in genetics and molecular biology and as tools in genetic engineering and biotechnology. Now we also recognize their huge role in natural environment and their importance in human health and disease. Despite our understanding of bacteriophage mechanisms of development, these viruses are described as parasites or predators in the literature. From the biological point of view, there are fundamental differences betw

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Schrader, Holly S., John O. Schrader, Jeremy J. Walker, Thomas A. Wolf, Kenneth W. Nickerson, and Tyler A. Kokjohn. "Bacteriophage infection and multiplication occur inPseudomonas aeruginosastarved for 5 years." Canadian Journal of Microbiology 43, no. 12 (December 1, 1997): 1157–63. http://dx.doi.org/10.1139/m97-164.

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Bacteriophages specific for Pseudomonas aeruginosa and Escherichia coli were examined for their ability to multiply in stationary phase hosts. Four out of five bacteriophages tested, including E. coli bacteriophage T7M, were able to multiply in stationary phase hosts. The bacteriophage ACQ had a mean burst size of approximately 1000 in exponential phase P. aeruginosa hosts and 102 in starved hosts, with corresponding latent periods that increased from 65 to 210 min. The bacteriophage UT1 had a mean burst size of approximately 211 in exponential phase P. aeruginosa hosts and 11 in starved hosts

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Klein, Gracjana, and Costa Georgopoulos. "Identification of Important Amino Acid Residues That Modulate Binding of Escherichia coli GroEL to Its Various Cochaperones." Genetics 158, no. 2 (June 1, 2001): 507–17. http://dx.doi.org/10.1093/genetics/158.2.507.

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Abstract Genetic experiments have shown that the GroEL/GroES chaperone machine of Escherichia coli is absolutely essential, not only for bacterial growth but also for the propagation of many bacteriophages including λ. The virulent bacteriophages T4 and RB49 are independent of the host GroES function, because they encode their own cochaperone proteins, Gp31 and CocO, respectively. E. coli groEL44 mutant bacteria do not form colonies above 42° nor do they propagate bacteriophages λ, T4, or RB49. We found that the vast majority (40/46) of spontaneous groEL44 temperature-resistant colonies at 43°

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Brüggemann, Holger, and Rolf Lood. "Bacteriophages InfectingPropionibacterium acnes." BioMed Research International 2013 (2013): 1–10. http://dx.doi.org/10.1155/2013/705741.

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Viruses specifically infecting bacteria, or bacteriophages, are the most common biological entity in the biosphere. As such, they greatly influence bacteria, both in terms of enhancing their virulence and in terms of killing them. Since the first identification of bacteriophages in the beginning of the 20th century, researchers have been fascinated by these microorganisms and their ability to eradicate bacteria. In this review, we will cover the history of thePropionibacterium acnesbacteriophage research and point out how bacteriophage research has been an important part of the research onP. a

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Benedi, Vicente J., Miguel Regué, Sebastián Albertí, Silvia Camprubí, and Juan M. Tomás. "Influence of environmental conditions on infection of Klebsiella pneumoniae by two different types of bacteriophages." Canadian Journal of Microbiology 37, no. 4 (April 1, 1991): 270–75. http://dx.doi.org/10.1139/m91-042.

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The adsorption and efficiency of plating of bacteriophages FC3-1 and FC3-9 on Klebsiella pneumoniae C3 (serotype O1:K66) cells grown at different pHs and temperatures were quantitated. Bacteriophage FC3-1, with lipopolysaccharide as its bacterial receptor, showed a large decrease in efficiency of plating on bacteria grown at low pH or low temperature. Under the same conditions, no significant decrease in efficiency of plating was found for bacteriophage FC3-9, a phage requiring capsule and lipopolysaccharide for its adsorption and carrying capsule-depolymerizing activity. We demonstrate that K

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Verbeken, Gilbert, Isabelle Huys, Jean-Paul Pirnay, Serge Jennes, Nina Chanishvili, Jacques Scheres, Andrzej Górski, Daniel De Vos, and Carl Ceulemans. "Taking Bacteriophage Therapy Seriously: A Moral Argument." BioMed Research International 2014 (2014): 1–8. http://dx.doi.org/10.1155/2014/621316.

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The excessive and improper use of antibiotics has led to an increasing incidence of bacterial resistance. In Europe the yearly number of infections caused by multidrug resistant bacteria is more than 400.000, each year resulting in 25.000 attributable deaths. Few new antibiotics are in the pipeline of the pharmaceutical industry. Early in the 20th century, bacteriophages were described as entities that can control bacterial populations. Although bacteriophage therapy was developed and practiced in Europe and the former Soviet republics, the use of bacteriophages in clinical setting was neglect

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Gong, Chao, Spencer Heringa, Randhir Singh, Jinkyung Kim, and Xiuping Jiang. "Isolation and characterization of bacteriophages specific to hydrogen-sulfide-producing bacteria." Canadian Journal of Microbiology 59, no. 1 (January 2013): 39–45. http://dx.doi.org/10.1139/cjm-2012-0245.

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The objectives of this study were to isolate and characterize bacteriophages specific to hydrogen-sulfide-producing bacteria (SPB) from raw animal materials, and to develop a SPB-specific bacteriophage cocktail for rendering application. Meat, chicken offal, and feather samples collected from local supermarkets and rendering processing plants were used to isolate SPB (n = 142). Bacteriophages (n = 52) specific to SPB were isolated and purified from the above samples using 18 of those isolated SPB strains as hosts. The host ranges of bacteriophages against 5 selected SPB strains (Escherichia co

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Jończyk-Matysiak, Ewa, Marlena Kłak, Beata Weber-Dąbrowska, Jan Borysowski, and Andrzej Górski. "Possible Use of Bacteriophages Active againstBacillus anthracisand OtherB. cereusGroup Members in the Face of a Bioterrorism Threat." BioMed Research International 2014 (2014): 1–14. http://dx.doi.org/10.1155/2014/735413.

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Anthrax is an infectious fatal disease with epidemic potential. Nowadays, bioterrorism usingBacillus anthracisis a real possibility, and thus society needs an effective weapon to neutralize this threat. The pathogen may be easily transmitted to human populations. It is easy to store, transport, and disseminate and may survive for many decades. Recent data strongly support the effectiveness of bacteriophage in treating bacterial diseases. Moreover, it is clear that bacteriophages should be considered a potential incapacitative agent against bioterrorism using bacteria belonging toB. cereusgroup

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Crane, Adele, Joy Abaidoo, Gabriella Beltran, Danielle Fry, Colleen Furey, Noe Green, Ravneet Johal, et al. "The Complete Genome Sequence of the Staphylococcus Bacteriophage Metroid." G3 Genes|Genomes|Genetics 10, no. 9 (September 1, 2020): 2975–79. http://dx.doi.org/10.1534/g3.120.401365.

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Abstract Phages infecting bacteria of the genus Staphylococcus play an important role in their host’s ecology and evolution. On one hand, horizontal gene transfer from phage can encourage the rapid adaptation of pathogenic Staphylococcus enabling them to escape host immunity or access novel environments. On the other hand, lytic phages are promising agents for the treatment of bacterial infections, especially those resistant to antibiotics. As part of an ongoing effort to gain novel insights into bacteriophage diversity, we characterized the complete genome of the Staphylococcus bacteriophage

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Guidolin, A., and P. A. Manning. "Genetics of Vibrio cholerae and its bacteriophages." Microbiological Reviews 51, no. 2 (1987): 285–98. http://dx.doi.org/10.1128/mmbr.51.2.285-298.1987.

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Guidolin, A., and P. A. Manning. "Genetics of Vibrio cholerae and its bacteriophages." Microbiological Reviews 51, no. 2 (1987): 285–98. http://dx.doi.org/10.1128/mr.51.2.285-298.1987.

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Girones, Rosina, Juan T. Jofre, and Albert Bosch. "Isolation of marine bacteria with antiviral properties." Canadian Journal of Microbiology 35, no. 11 (November 1, 1989): 1015–21. http://dx.doi.org/10.1139/m89-169.

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We report in this study the isolation of marine bacteria with antiviral properties that have been tentatively classified as Moraxella. These bacteria retained their virucidal capacity after prolonged subcultivation in the laboratory. The virus-inactivating agent could not be separated from the viable marine bacteria, indicating that the active agent(s) either remains associated to the microorganisms or has a very short lifetime, or both. The antiviral capacity of the isolated microorganisms was highly specific for poliovirus. No virucidal effect was observed against other strains of enteroviru

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Rigvava, Sophio, Irina Tchgkonia, Darejan Jgenti, Teona Dvalidze, James Carpino, and Marina Goderdzishvili. "Comparative analysis of the biological and physical properties of Enterococcus faecalis bacteriophage vB_EfaS_GEC-EfS_3 and Streptococcus mitis bacteriophage vB_SmM_GEC-SmitisM_2." Canadian Journal of Microbiology 59, no. 1 (January 2013): 18–21. http://dx.doi.org/10.1139/cjm-2012-0385.

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Enterococcus faecalis and Streptococcus mitis are common commensal inhabitants of the human gastrointestinal and genitourinary tracts. However, both species can be opportunistic pathogens and cause disease in nosocomial settings. These infections can be difficult to treat because of the frequency of antibiotic resistance among these strains. Bacteriophages are often suggested as an alternative therapeutic agent against these infections. In this study, E. faecalis and S. mitis strains were isolated from female patients with urinary tract infections. Bacteriophages active against these strains w

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Śliwa-Dominiak, Joanna, Ewa Suszyńska, Małgorzata Pawlikowska, and Wiesław Deptuła. "Chlamydia bacteriophages." Archives of Microbiology 195, no. 10-11 (August 1, 2013): 765–71. http://dx.doi.org/10.1007/s00203-013-0912-8.

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BÃ¯Â¿Â½rsheim, Knut Yngve. "Native marine bacteriophages." FEMS Microbiology Letters 102, no. 3-4 (April 1993): 141–59. http://dx.doi.org/10.1111/j.1574-6968.1993.tb05805.x.

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Smith, Leslie A., and John W. Drake. "Aspects of the Ultraviolet Photobiology of Some T-Even Bacteriophages." Genetics 148, no. 4 (April 1, 1998): 1611–18. http://dx.doi.org/10.1093/genetics/148.4.1611.

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Abstract Bacteriophage T4 DNA metabolism is largely insulated from that of its host, although some host functions assist in the repair of T4 DNA damage. Environmental factors sometimes affect survival and mutagenesis after ultraviolet (UV) irradiation of T4, and can affect mutagenesis in many organisms. We therefore tested the effect of certain environmental factors and host genetic defects upon spontaneous and UV-induced mutagenesis and survival in T4 and some related T-even phages. Plating at pH 9 enhances UV resistance in T4 by about 14% compared to pH 7. The host cAMP regulatory system aff

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Arsuaga, J., and Y. Diao. "DNA Knotting in Spooling Like Conformations in Bacteriophages." Computational and Mathematical Methods in Medicine 9, no. 3-4 (2008): 303–16. http://dx.doi.org/10.1080/17486700802167801.

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A number of idealized models have been proposed to explain the long range organization of the DNA in bacteriophages. However, none of these models can account for the distributions of complex knots found when examining DNA extracted from bacteriophage P4 capsids. Furthermore, these models do not consider possible chirality biases in the arrangement of the DNA molecule inside the capsid. In this paper, we address these two issues by proposing a randomized version of one of the most popular models: the coaxially spooled model. We present analytical and numerical results for the properties of the

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Armon, Robert, Max Arella, and Pierre Payment. "A highly efficient second-step concentration technique for bacteriophages and enteric viruses using ammonium sulfate and Tween 80." Canadian Journal of Microbiology 34, no. 5 (May 1, 1988): 651–55. http://dx.doi.org/10.1139/m88-107.

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Addition of Tween 80 to a 1.5% solution of beef extract was found to enhance the elution of bacteriophages adsorbed to electronegative filters. When reconcentration of the eluate was attempted by ammonium sulfate precipitation, a floating layer containing most of the viruses was formed. This floating layer can be obtained with several nonionic detergents including Tween 80 and under a salt saturation of 55% with ammonium sulfate, potassium tartrate, and sodium phosphate. Virus recovery ranged from 91 to 103% and was obtained with several bacteriophage strains. With poliovirus type 1, coxsackie

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Isaev, Artem, Alena Drobiazko, Nicolas Sierro, Julia Gordeeva, Ido Yosef, Udi Qimron, Nikolai V. Ivanov, and Konstantin Severinov. "Phage T7 DNA mimic protein Ocr is a potent inhibitor of BREX defence." Nucleic Acids Research 48, no. 10 (April 27, 2020): 5397–406. http://dx.doi.org/10.1093/nar/gkaa290.

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Abstract BREX (for BacteRiophage EXclusion) is a superfamily of common bacterial and archaeal defence systems active against diverse bacteriophages. While the mechanism of BREX defence is currently unknown, self versus non-self differentiation requires methylation of specific asymmetric sites in host DNA by BrxX (PglX) methyltransferase. Here, we report that T7 bacteriophage Ocr, a DNA mimic protein that protects the phage from the defensive action of type I restriction–modification systems, is also active against BREX. In contrast to the wild–type phage, which is resistant to BREX defence, T7

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Dabrowski, Tomasz, and Bartlomiej Kwiatkowski. "Sensitivity of Vi phages III to gamma-radiation in the presence of cisplatin." Acta Biochimica Polonica 52, no. 2 (May 31, 2005): 545–50. http://dx.doi.org/10.18388/abp.2005_3471.

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In this study we determined Vi bacteriophage III sensitivity to native cisplatin, gamma radiation ((60)Co) or to irradiated cisplatin, and checked the possibility of enhanced Vi bacteriophage III inactivation under combined exposure to cisplatin and gamma radiation. We used highly purified phage suspensions in 0.9% NaCl solution or phosphate-buffered saline. Phage suspensions were titrated using a double agar layer method. Our study implies that survival of Vi bacteriophage III shows an exponential inverse correlation with cisplatin concentration in the incubation medium and the time of phage

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Arendt, E. K., M. van de Guchte, A. G. Coffey, C. Daly, and G. F. Fitzgerald. "Molecular genetics of bacteriophages of lactic acid bacteria." Le Lait 73, no. 2 (1993): 191–98. http://dx.doi.org/10.1051/lait:1993217.

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Rokyta, Darin R., Zaid Abdo, and Holly A. Wichman. "The Genetics of Adaptation for Eight Microvirid Bacteriophages." Journal of Molecular Evolution 69, no. 3 (August 20, 2009): 229–39. http://dx.doi.org/10.1007/s00239-009-9267-9.

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Sackman, Andrew M., and Darin R. Rokyta. "No Cost of Complexity in Bacteriophages Adapting to a Complex Environment." Genetics 212, no. 1 (February 26, 2019): 267–76. http://dx.doi.org/10.1534/genetics.119.302029.

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Benini, Stefano, Maria Chechik, Miguel Ortiz Lombardía, Sigrun Polier, Andrew Leech, Mikhail B. Shevtsov, and Juan C. Alonso. "The 1.58 Å resolution structure of the DNA-binding domain of bacteriophage SF6 small terminase provides new hints on DNA binding." Acta Crystallographica Section F Structural Biology and Crystallization Communications 69, no. 4 (March 28, 2013): 376–81. http://dx.doi.org/10.1107/s1744309113004399.

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DNA packaging in tailed bacteriophages and in evolutionarily related herpesviruses is controlled by a viral-encoded terminase. As in a number of other phages, in theBacillus subtilisbacteriophages SF6 and SPP1 the terminase complex consists of two proteins: G1P and G2P. The crystal structure of the N-terminal DNA-binding domain of the bacteriophage SF6 small terminase subunit G1P is reported. Structural comparison with other DNA-binding proteins allows a general model for the interaction of G1P with the packaging-initiation site to be proposed.

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Farfán, Juan, John M. Gonzalez, and Martha Vives. "The immunomodulatory potential of phage therapy to treat acne: a review on bacterial lysis and immunomodulation." PeerJ 10 (July 25, 2022): e13553. http://dx.doi.org/10.7717/peerj.13553.

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Background Characterized by an inflammatory pathogenesis, acne is the most common skin disorder worldwide. Altered sebum production, abnormal proliferation of keratinocytes, and microbiota dysbiosis represented by disbalance in Cutibacterium acnes population structure, have a synergic effect on inflammation of acne-compromised skin. Although the role of C. acnes as a single factor in acne development is still under debate, it is known that skin and skin-resident immune cells recognize this bacterium and produce inflammatory markers as a result. Control of the inflammatory response is frequentl

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Efimov, Alexander, Eugene Kulikov, Alla Golomidova, Ilya Belalov, Vladislav Babenko, and Andrey Letarov. "Isolation and sequencing of three RB49-like bacteriophages infecting O antigen-producing E. coli strains." F1000Research 10 (November 3, 2021): 1113. http://dx.doi.org/10.12688/f1000research.74169.1.

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E. coli strains 4s, F5 and F17, whose O antigens are structurally characterized and shown to effectively shield the cell surface from bacteriophage attack, were used as the hosts to isolate novel RB49-like bacteriophages. Three novel phage isolates were obtained, and their genomes were sequenced and annotated. Despite high overall identity levels of these genomic sequences, the variants of large distal tail fiber subunit, orthologous to the bacteriophage T2 long tail receptor recognition protein gp38, were unique for each phage, suggesting their role in host range determination. The annotated

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Zhukova, O. V., E. I. Kasikhina, M. N. Ostretsova, and A. A. M. Nemer. "Bacteriophages in the treatment and prevention of atopic dermatitis and dermatoses complicated by secondary bacterial infection." Meditsinskiy sovet = Medical Council, no. 13 (August 9, 2022): 66–72. http://dx.doi.org/10.21518/2079-701x-2022-16-13-66-72.

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Bacteriophages are a large group of viruses that can selectively affect bacteria. Bacteriophages and their ability to regulate the growth and activity of pathogenic microorganisms were discovered by scientists at the beginning of the 20th century. Further studies of the properties of bacteriophages led to the construction of the modern concept of virus activity and formed the ground of molecular genetics and biology. To date, more than 6 000 phage species are known to be ubiquitous, but a prerequisite for their existence is the presence of a bacterial host cell, proteins and energy resources s

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Cheleuitte-Nieves, Christopher, Ryan D. Heselpoth, Lars F. Westblade, Neil S. Lipman, and Vincent A. Fischetti. "Searching for a Bacteriophage Lysin to Treat Corynebacterium bovis in Immunocompromised Mice." Comparative Medicine 70, no. 4 (August 1, 2020): 328–35. http://dx.doi.org/10.30802/aalas-cm-19-000096.

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Corynebacterium bovis is the causative agent of Corynebacterium-associated hyperkeratosis in immunocompromised mice. The resulting skin pathology can be profound and can be associated with severe wasting, making the animals unsuitable for research. Although the administration of antibiotics is effective in resolving clinical symptoms, antibiotics do not eradicate the offending bacterium. Furthermore, antibiotic use may be contraindicated as it can affect tumor growth and is associated with Clostridioides difficile enterotoxemia in highly immunocompromised murine strains. Lysins, which are lyti

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Vasse, Marie, and Sébastien Wielgoss. "Bacteriophages of Myxococcus xanthus, a Social Bacterium." Viruses 10, no. 7 (July 18, 2018): 374. http://dx.doi.org/10.3390/v10070374.

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Bacteriophages have been used as molecular tools in fundamental biology investigations for decades. Beyond this, however, they play a crucial role in the eco-evolutionary dynamics of bacterial communities through their demographic impact and the source of genetic information they represent. The increasing interest in describing ecological and evolutionary aspects of bacteria–phage interactions has led to major insights into their fundamental characteristics, including arms race dynamics and acquired bacterial immunity. Here, we review knowledge on the phages of the myxobacteria with a major fo

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Yan, Jianlong, Xiangyu Fan, and Jianping Xie. "Emerging Biomedicines Based on Bacteriophages." Critical Reviews in Eukaryotic Gene Expression 23, no. 4 (2013): 299–308. http://dx.doi.org/10.1615/critreveukaryotgeneexpr.2013006578.

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Wulff, Daniel L., and Michael E. Mahoney. "Cross-Specificities Between cII-like Proteins and pRE-like Promoters of Lambdoid Bacteriophages." Genetics 115, no. 4 (April 1, 1987): 597–604. http://dx.doi.org/10.1093/genetics/115.4.597.

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ABSTRACT We have investigated the activation of transcription from the pRE promoters of phages λ, 21 and P22 by the λ and 21 cII proteins and the P22 c1 (cII-like) protein, using an in vivo system in which cII protein from a derepressed prophage activates transcription from a pRE DNA fragment on a multicopy plasmid. We find that each protein is highly specific for its own cognate pRE promoter, although measureable cross-reactions are observed. The primary recognition sequence for cII protein on λ pRE is a pair of TTGC repeat sequences in the sequence 5′-TTGCN6TTGC-3′ at the -35 region of the p

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Wood, Jonathan P. A., Stephanie A. Capaldi, Mark A. Robinson, Andrew J. Baron та Nicola J. Stonehouse. "RNA Multimerisation in the DNA Packaging Motor of Bacteriophage φ29". Journal of Theoretical Medicine 6, № 2 (2005): 127–34. http://dx.doi.org/10.1080/10273660500149802.

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The use of bacteriophages as experimental tools allows the investigation of interactions between components at the molecular level that are often not possible in more complex virus systems. The bacteriophage φ29 acts as a molecular machine to package its own genomic DNA during viral assembly. Self-associating RNA molecules, called pRNA, have an essential role in the function of this machine. This paper reports the characterization of this self-association (which leads to multimerisation of wild-type and truncated variant pRNAs) by analytical ultracentrifugation (including determination of the

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Pleteneva, E. A., M. V. Bourkaltseva, O. V. Shaburova, S. V. Krylov, E. V. Pechnikova, O. S. Sokolova, and V. N. Krylov. "TL, the new bacteriophage of Pseudomonas aeruginosa and its application for the search of halo-producing bacteriophages." Russian Journal of Genetics 47, no. 1 (January 2011): 1–5. http://dx.doi.org/10.1134/s1022795411010091.

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Klucar, Lubos, Matej Stano, and Matus Hajduk. "phiSITE: database of gene regulation in bacteriophages." Nucleic Acids Research 38, suppl_1 (November 7, 2009): D366—D370. http://dx.doi.org/10.1093/nar/gkp911.

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Alam, Muntasir, Marufa Zerin Akhter, Mahmuda Yasmin, Chowdhury Rafiqul Ahsan, and Jamalun Nessa. "Local bacteriophage isolates showed anti-Escherichia coliO157:H7 potency in an experimental ligated rabbit ileal loop model." Canadian Journal of Microbiology 57, no. 5 (May 2011): 408–15. http://dx.doi.org/10.1139/w11-020.

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Escherichia coli O157:H7 is considered among the most important recently emerged food-borne bacteria causing severe hemorrhagic diarrhea. Antibiotic treatment is not recommended as a prospective curative agent against this pathogen. Therefore, potency assessment of the local lytic phage isolates infecting E. coli O157:H7 as an alternate remedy to antibiotics was the principal concern of this study. Phage isolates against E. coli O157:H7 were checked by polymerase chain reaction for the presence of the virulence genes stx1 and stx2, and the safe phages were further screened in vitro for their c

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Pertics, Botond Zsombor, Dalma Szénásy, Dániel Dunai, Yannick Born, Lars Fieseler, Tamás Kovács, and György Schneider. "Isolation of a Novel Lytic Bacteriophage against a Nosocomial Methicillin-Resistant Staphylococcus aureus Belonging to ST45." BioMed Research International 2020 (December 22, 2020): 1–10. http://dx.doi.org/10.1155/2020/5463801.

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Methicillin-resistant Staphylococcus aureus (MRSA) can cause a wide range of infections from mild to life-threatening conditions. Its enhanced antibiotic resistance often leads to therapeutic failures and therefore alternative eradication methods must be considered. Potential candidates to control MRSA infections are bacteriophages and their lytic enzymes, lysins. In this study, we isolated a bacteriophage against a nosocomial MRSA strain belonging to the ST45 epidemiologic group. The phage belonging to Caudovirales, Siphoviridae, showed a narrow host range and stable lytic activity without th

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Kalmokofff, M. L., and D. E. Bradley. "Contractile-tailed bacteriophages adsorb toEscherichia coliO128ab lipopolysaccharide that is altered by large plasmids to provide receptors and lipopolysaccharide heterogeneity within the serogroup." Canadian Journal of Microbiology 41, no. 2 (February 1, 1995): 163–69. http://dx.doi.org/10.1139/m95-022.

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The verotoxigenic Escherichia coli strain H.I.8 (originally O128:B12, now not typeable) contained a ColB+M plasmid and two morphologically identical temperate bacteriophages (HI8A and HI8B). Both phages were O128ab specific, using the lipopolysaccharide O side chains of susceptible clinical isolates as receptors. SDS polyacrylamide gel electrophoresis with silver staining of O128ab lipopolysaccharide revealed four distinct types of ladder with different interband spacings. No specificity was found between ladder type and sensitivity to either phage. One of the numerous large plasmids present i

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Moon, Choi, Jeong, Sohn, Han, and Oh. "Research Progress of M13 Bacteriophage-Based Biosensors." Nanomaterials 9, no. 10 (October 11, 2019): 1448. http://dx.doi.org/10.3390/nano9101448.

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Recently, new virus-based sensor systems that operate on M13 bacteriophage infrastructure have attracted considerable attention. These systems can detect a range of chemicals with excellent sensitivity and selectivity. Filaments consistent with M13 bacteriophages can be ordered by highly established forms of self-assembly. This allows M13 bacteriophages to build a homogeneous distribution and infiltrate the network structure of nanostructures under mild conditions. Phage display, involving the genetic engineering of M13 bacteriophages, is another strong feature of the M13 bacteriophage as a fu

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Newlon, C. S., L. R. Lipchitz, I. Collins, A. Deshpande, R. J. Devenish, R. P. Green, H. L. Klein, T. G. Palzkill, R. B. Ren, and S. Synn. "Analysis of a circular derivative of Saccharomyces cerevisiae chromosome III: a physical map and identification and location of ARS elements." Genetics 129, no. 2 (October 1, 1991): 343–57. http://dx.doi.org/10.1093/genetics/129.2.343.

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Abstract DNA was isolated from a circular derivative of chromosome III to prepare a library of recombinant plasmids enriched in chromosome III sequences. An ordered set of recombinant plasmids and bacteriophages carrying the contiguous 210-kilobase region of chromosome III between the HML and MAT loci was identified, and a complete restriction map was prepared with BamHI and EcoRI. Using the high frequency transformation assay and extensive subcloning, 13 ARS elements were mapped in the cloned region. Comparison of the physical maps of chromosome III from three strains revealed that the chromo

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Zinder, Norton D. "Single-stranded DNA-containing bacteriophages." BioEssays 5, no. 2 (August 1986): 84–87. http://dx.doi.org/10.1002/bies.950050209.

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Karamoddini, M. Khajeh, B. S. Fazli-Bazzaz, F. Emamipour, M. Sabouri Ghannad, A. R. Jahanshahi, N. Saed, and A. Sahebkar. "Antibacterial Efficacy of Lytic Bacteriophages against Antibiotic-ResistantKlebsiellaSpecies." Scientific World JOURNAL 11 (2011): 1332–40. http://dx.doi.org/10.1100/tsw.2011.114.

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Bacterial resistance to antibiotics is a leading and highly prevalent problem in the treatment of infectious diseases. Bacteriophages (phages) appear to be effective and safe alternatives for the treatment of resistant infections because of their specificity for bacterial species and lack of infectivity in eukaryotic cells. The present study aimed to isolate bacteriophages againstKlebsiellaspp. and evaluate their efficacy against antibiotic-resistant species. Seventy-two antibiotic-resistantKlebsiellaspp. were isolated from samples of patients who referred to the Ghaem Hospital (Mashhad, Iran)

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Gross, Michael. "Revived interest in bacteriophages." Current Biology 21, no. 8 (April 2011): R267—R270. http://dx.doi.org/10.1016/j.cub.2011.04.008.

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Carey-Smith, Gwyneth V., Craig Billington, Angela J. Cornelius, J. Andrew Hudson, and Jack A. Heinemann. "Isolation and characterization of bacteriophages infectingSalmonellaspp." FEMS Microbiology Letters 258, no. 2 (May 2006): 182–86. http://dx.doi.org/10.1111/j.1574-6968.2006.00217.x.

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Shaburova, O. V., K. Hertveldt, D. M. A. de la Cruz, S. V. Krylov, E. A. Pleteneva, M. V. Bourkaltseva, R. Lavigne, G. Volckaert та V. N. Krylov. "Comparison of new giant bacteriophages OBP and Lu11 of soil pseudomonads with bacteriophages of the ϕKZ-supergroup of Pseudomonas aeruginosa". Russian Journal of Genetics 42, № 8 (серпень 2006): 877–85. http://dx.doi.org/10.1134/s1022795406080059.

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Jarvis, A. W. "Bacteriophages of Lactic Acid Bacteria." Journal of Dairy Science 72, no. 12 (December 1989): 3406–28. http://dx.doi.org/10.3168/jds.s0022-0302(89)79504-7.

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Fillol-Salom, Alfred, Ahlam Alsaadi, Jorge A. Moura de Sousa, Li Zhong, Kevin R. Foster, Eduardo P. C. Rocha, José R. Penadés, Hanne Ingmer, and Jakob Haaber. "Bacteriophages benefit from generalized transduction." PLOS Pathogens 15, no. 7 (July 5, 2019): e1007888. http://dx.doi.org/10.1371/journal.ppat.1007888.

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Kazibwe, George, Phionah Katami, Ruth Alinaitwe, Stephen Alafi, Ann Nanteza, and Jesca Lukanga Nakavuma. "Bacteriophage activity against and characterisation of avian pathogenic Escherichia coli isolated from colibacillosis cases in Uganda." PLOS ONE 15, no. 12 (December 15, 2020): e0239107. http://dx.doi.org/10.1371/journal.pone.0239107.

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Avian Pathogenic Escherichia coli (APEC) cause colibacillosis leading to significant economic losses in the poultry industry. This laboratory-based study aimed at establishing stocks of avian pathogenic Escherichia coli lytic bacteriophages, for future development of cocktail products for colibacillosis management. The study determined the antibiotic susceptibility; phylogenetic categories, occurrence of selected serotypes and virulence genes among Escherichia coli stock isolates from chicken colibacillosis cases; and evaluated bacteriophage activity against the bacteria. Escherichia coli char

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Rahmat Ullah, Sidra, Saadia Andleeb, Taskeen Raza, Muhsin Jamal, and Khalid Mehmood. "Effectiveness of a Lytic Phage SRG1 against Vancomycin-ResistantEnterococcus faecalisin Compost and Soil." BioMed Research International 2017 (2017): 1–8. http://dx.doi.org/10.1155/2017/9351017.

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Nosocomial infections caused by vancomycin-resistantEnterococcushave become a major problem. Bacteriophage therapy is proposed as a potential alternative therapy. Bacteriophages are viruses that infect bacteria and are ubiquitous in nature. Lytic bacteriophage was isolated from sewage water that infects VREF, the causative agent of endocarditis, bacteraemia, and urinary tract infections (UTIs). The phage produced clear plaques with unique clear morphology and well-defined boundaries. TEM results of phage revealed it to be108±0.2 nm long and90±0.5 nm wide. The characterization of bacteriophage

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