Bibliographies : « Benzyl aminopurine »

1

A, KALAMANI. "INITIATION OF CALLUS AND PLANT REGENERATION IN COTTON (Gossypium hirsutum L.)." Madras Agricultural Journal 81, November (1994): 579–80. http://dx.doi.org/10.29321/maj.10.a01585.

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The hypocotyl explants of MDU 9 cultured on Murashige and Skoog medium supplemented with kinetin (1.0 mg/lit.)+ IAA (1.5 mg/lit.) formed callus in 20 days. The calli on sub culture to media containing benzyl amino purine (2.0 mg/lit.) developed shoots. Multiple shoots were formed from each callus mass. These on further sub culture with reduced level of benzyl aminopurine developed well. Root initiation and growth was promoted by lowering the sucrose concentration and supplementing the culture media with NAA (3.0 mg/lit.).

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Nada, Shadia, Siva Chennareddy, Stephen Goldman, et al. "Direct Shoot Bud Differentiation and Plantlet Regeneration from Leaf and Petiole Explants of Begonia tuberhybrida." HortScience 46, no. 5 (2011): 759–64. http://dx.doi.org/10.21273/hortsci.46.5.759.

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We report here an efficient and high-frequency protocol for direct shoot bud differentiation from mature leaves and petioles of greenhouse-grown Begonia tuberhybrida plants. Shoot buds were induced directly on the adaxial surface of leaf tissues from not only at the cut ends, but also across the entire surface of both leaf and petiole segments. The highest frequency of shoot bud formation was 90%, and the maximum number of shoots (132) per leaf explant was achieved with modified Murashige and Skoog (MS) (Murashige and Skoog, 1962) media supplemented with 1.0 mg·L−1 α-naphthalene acetic acid (NAA) and 2.0 mg·L−1 thidiazuron (TDZ). In petioles, the highest frequency of shoot buds was 82%. A maximum number of 33 shoots per explant was achieved with 0.5 mg·L−1 NAA and 2.0 mg·L−1 TDZ. The number of shoots produced in both explants was drastically reduced in the treatment with benzyl-aminopurine (BAP) alone or in combination with NAA and/or TDZ. The regenerated shoots were rooted on MS medium supplemented with 0.5 mg·L−1 NAA. All the elongated shoots developed into complete, rooted plantlets within 3 months. All the plantlets were successfully transferred to soil in pots in the greenhouse and they produced morphologically normal flowers. Chemical names used: α-naphthalene acetic acid (NAA), N-phenyl-N′-1,2,3-thiadiazol-5ylurea (TDZ; thidiazuron), 6-benzyl aminopurine (BAP)

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Nur Khozin, Mohammad, and Muhammad Dima Say Mona. "Induction and Regeneration of Tobacco Callus using Benzyl Aminopurine (BAP) and Furfuryl Aminopurine (Kinetin) With Thin Cell Layer (TCL) Technique." IOP Conference Series: Earth and Environmental Science 1497, no. 1 (2025): 012017. https://doi.org/10.1088/1755-1315/1497/1/012017.

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Abstract Tobacco was a high-value crop that required effective and efficient cultivation methods to produce quality seedlings. This study aimed to enhance the efficiency of tobacco cultivation through tissue culture using the Thin Cell Layer (TCL) method. The study took place at the Tissue Culture Laboratory, Faculty of Agriculture, University of Jember, between January and March 2024. Leaf bud explants from the Broadleaf One-Sucker tobacco variety were cultivated on Murashige & Skoog (MS) medium supplemented with different concentrations of BAP (0, 2, 3, 4 ppm) and Kinetin (0, 3, 4 ppm). The study employed a Completely Randomized Design (CRD) and was analyzed using SPSS 25 and Microsoft Excel. Callus color analysis used the Munsell Color Chart. The findings revealed that 3 ppm BAP was the best concentration for inducing callus in tobacco, with the callus appearing 10.78 days after planting (DAP) and explants sprouting within 8.3 DAP, producing 81.3 shoots. Visual observations indicated that the combination of 3 ppm BAP and 4 ppm Kinetin (P2M2) produced green callus with a compact texture, suitable for shoot formation. The green color of the callus indicated chlorophyll content, which was important for photosynthesis and shoot growth.

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Bonello, Mariella, Uroš Gašić, Živoslav Tešić, and Everaldo Attard. "Production of Stilbenes in Callus Cultures of the Maltese Indigenous Grapevine Variety, Ġellewża." Molecules 24, no. 11 (2019): 2112. http://dx.doi.org/10.3390/molecules24112112.

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The production of secondary metabolites in tissue culture has been considered as an alternative to the cultivation and harvesting of crops intended for this purpose. The present study was aimed at the growth of callus and production of polyphenolic compound of callus derived from a Maltese indigenous grapevine variety, Ġellewża. Callus was inoculated onto plant growth regulators-enriched Murashige Skoog media (MSm) to determine whether polyphenols are produced in vitro as well as to determine the best combination of plant growth regulators needed for the production of these metabolites. From results obtained, it was observed that the best callus production was obtained by auxin-enriched MSm. In fact, indole acetic acid and indole acetic acid /6-benzyl aminopurine enhanced biomass accumulation (3.04 g and 3.39 g) as opposed to the others (<1.97 g). On the other hand, parameters showing the presence of flavonoids (tonality, 3.80), particularly anthocyanins (24.09 mg/kg) and total polyphenols (1.42 mg/g), were optimum in the presence of cytokinins, particularly 6-benzyl aminopurine. Analysis for single polyphenols revealed a high amount a particular stilbene: polydatin (glucoside of resveratrol). Resveratrol and other typical polyphenols, found in mature berries, were also found in significant quantities, while the other polyphenolic compounds were found in minimal quantities. This is the first study to describe the production and composition of polyphenols in Ġellewża callus cultures. From the results obtained, it can be seen that this grape tissue is an excellent alternative for the production of polyphenols from the stilbene group, which can be upscaled and exploited commercially.

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Rustikawati, Rustikawati, Catur Herison, Entang Inoriah, and Vera Dwisari. "Effect of BAP (6-Benzyl Aminopurine) on In Vitro Shoot Growth of Curcumas." AGRITROPICA : Journal of Agricultural Sciences 4, no. 1 (2021): 82–92. http://dx.doi.org/10.31186/j.agritropica.4.1.82-92.

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Curcuma sp has been widely investigated for its anti-cancer properties. Conventionally, vegetative propagation needs a long time to produce a large number of planting materials, so that it is necessary to find an alternative approach through in vitro propagation. The effect of BAP on the in vitro shoot formation of ‘temu putih’ and ‘temu putih’ has been investigated in this study. The experiment was a 4x2 factorial with 5 replications arranged in a completely randomized design. The first factor was the concentration of BAP i.e. 0, 1.5, 3, and 4.5 ppm. The second factor was the curcuma species consisting of ‘temu putih’ (Curcuma zedoaria Roch.) and ‘temu mangga’ (Curcuma mangga Val.). Each experimental unit consisted of 2 in vitro bottles, each of which planted with 1 explant bud. Analysis of variance was conducted on percentage of live explants, shoot height, number of roots, root length, wet weight, percentage of explants that sprouted, percentage of rooted explants and shoot color. Mean comparison was performed by the Least Significant Difference (LSD). The results showed that there was no interaction between BAP concentration and genotype on any variable observed. The shoot growth of ‘temu putih’ was significantly higher than ‘temu mangga’ in vitro. The best concentration of BAP for the growth of ‘temu putih’ and ‘temu mangga’ shoots was 1.5 ppm.

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Markovic, Marija, Mihailo Grbic, and Ana Sindelic. "Possibility of micropropagation of dianthus giganteiformis subsp. Kladovanus (Degen) SÓO by the method of proliferation of lateral shoots." Bulletin of the Faculty of Forestry, no. 94 (2006): 171–80. http://dx.doi.org/10.2298/gsf0694171m.

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The micropropagation of kladovanus carnation (Dianthus giganteiformis subsp kladovanus) was tested by the lateral shoot proliferation method. Micropropagation was performed on the medium with 1/2 MS mineral solution supplemented with 3% sucrose, 0.8% agar, 50 mg?L-1 mio-inositol, 0.025 mg?L-1 thiamin, 0.125 mg?L-1 nicotinic acid and 0.5 mg?L-1 glycine. The development of lateral shoots was induced on 6-benzyl-aminopurine (BAP) and ?-naphthaleneacetic acid (NAA). The best results were achieved on the medium with 1 mg?L-1 BAP and 0.1 mg?L-1 NAA. Rooting was successful on the medium without hormones, and the percentage of acclimatisation was 83%.

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Markovic, Marija, Mihailo Grbic, Dragana Skocajic, and Danijela Djunisijevic-Bojovic. "Effect of phytohormone balance on shoot multiplication and rooting of the species Dianthus serotinus Waldst. and Kit." Bulletin of the Faculty of Forestry, no. 95 (2007): 83–94. http://dx.doi.org/10.2298/gsf0795083m.

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The effect of different phytohormone concentrations on shoot multiplication and shoot rooting was researched on the species Dianthus serotinus. All nutritive media contained MS mineral solution, 3% sucrose, 0.8% agar, 100 mg?L-1 mioinositol, 0.05 mg?L-1 thiamin, 0.25 mg?L-1 nicotinic acid and 1 mg?L-1 glycine. For lateral shoot proliferation, the basic medium was supplemented with BAP (6-benzyl-aminopurine) in concentrations 0.1-2 mg?L-1 and NAA (?-naphthalene-acetic acid) in concentrations 0.1-1 mg?L-1. The best results were achieved on the medium with 0.5 mg?L-1 BAP and NAA. Rooting was most successful (73%) on the medium with 0.5 mg?L-1 NAA.

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Afzal Hossain, Md. "Standardization of Sucrose and 6-Benzyl Aminopurine for in vitro Micro Tuberization of Potato." American Journal of Agriculture and Forestry 3, no. 2 (2015): 25. http://dx.doi.org/10.11648/j.ajaf.20150302.13.

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Pandey, Ved Prakash, Jose Kudakasseril, Elizabeth Cherian, and George Patani. "COMPARISON OF TWO METHODS FOR IN VITRO PROPAGATION OF RAUWOLFIA SERPENTINA FROM NODAL EXPLANTS." INDIAN DRUGS 44, no. 07 (2007): 514–19. http://dx.doi.org/10.53879/id.44.07.p0514.

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Two different methods of in vitro multiplication of Rauwolfia serpentina from nodal explants were compared viz. multiplication via callus morphogenesis and that via shoot proliferation from axillary buds. The second method was found to be far better. The optimum shoot proliferation occurred on Murashige and Skoog (MS) medium supplemented with 1 mg/L naphthalene acetic acid (NAA) and 2 mg/L of benzyl aminopurine (BAP). The best rooting of shoots occurred on MS medium containing 4% sucrose and 1 mg/L of NAA. Solid and liquid MS media were found to be similar in supporting shoot proliferation. The plants produced were successfully hardened and established in soil. An easy, reliable and reproducible protocol was developed for in vitro micropropagation of Rauwolfia serpentina from nodal explants.

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Pandey, Ved Prakash, Jose Kudakasseril, Elizabeth Cherian, and George Patani. "Comparison of two methods for in vitro propagation of Rauwolfia serpentina from nodal explants." Indian Drugs 44, no. 7 (2007): 514–19. https://doi.org/10.5281/zenodo.3597219.

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Two different methods of in vitro multiplication of Rauwolfia serpentina from nodal explants were compared viz. multiplication via callus morphogenesis and that via shoot proliferation from axillary buds. The second method was found to be far better. The optimum shoot proliferation occurred on Murashige and Skoog (MS) medium supplemented with 1 mg/L naphthalene acetic acid (NAA) and 2 mg/L of benzyl aminopurine (BAP). The best rooting of shoots occurred on MS medium containing 4% sucrose and 1 mg/L of NAA. Solid and liquid MS media were found to be similar in supporting shoot proliferation. The plants produced were successfully hardened and established in soil. An easy, reliable and reproducible protocol was developed for in vitro micropropagation of Rauwolfia serpentina from nodal explants.

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