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Littérature scientifique sur le sujet « Charged-residue »

Auteur : Grafiati

Publié le 9 mars 2023

Mis à jour le 31 juillet 2025

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Articles de revues sur le sujet "Charged-residue"

Armstrong, K. M., and R. L. Baldwin. "Charged histidine affects alpha-helix stability at all positions in the helix by interacting with the backbone charges." Proceedings of the National Academy of Sciences 90, no. 23 (1993): 11337–40. http://dx.doi.org/10.1073/pnas.90.23.11337.

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To determine whether a charged histidine side chain affects alpha-helix stability only when histidine is close to one end of the helix or also when it is in the central region, we substitute a single histidine residue at many positions in two reference peptides and measure helix stability and histidine pKa. The position of a charged histidine residue has a major effect on helix stability in 0.01 M NaCl: the helix content of a 17-residue peptide is 24% when histidine is at position 3 compared to 76% when it is at position 17. This dependence of helix content on histidine position decreases shar

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Zhang, Zhao, Yanfang Xu, Pei Hong Dong, Dipika Sharma, and Nipavan Chiamvimonvat. "A negatively charged residue in the outer mouth of rat sodium channel determines the gating kinetics of the channel." American Journal of Physiology-Cell Physiology 284, no. 5 (2003): C1247—C1254. http://dx.doi.org/10.1152/ajpcell.00471.2002.

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Previous studies using combined techniques of site-directed mutagenesis and electrophysiology of voltage-gated Na+ channels have demonstrated that there are significant overlaps in the regions that are important for the two fundamental properties of the channels, namely gating and permeation. We have previously shown that a pore-lining residue, W402 in S5-S6 region (P loop) in domain I of the μ1 skeletal muscle Na+channel, was important in the gating of the channel. Here, we determined the role of an adjacent pore-lining negatively charged residue (E403) in channel gating. Charge neutralizatio

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Lemire, Isabelle, Patrice Roy, and Guy Boileau. "Translocation of neutral endopeptidase 24.11 mutants with deletions of the NH2- terminal cytosolic domain." Biochemistry and Cell Biology 72, no. 5-6 (1994): 182–87. http://dx.doi.org/10.1139/o94-027.

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Rabbit neutral endopeptidase 24.11 (NEP) is a type II membrane protein with a positively charged 27 amino acid residue NH2-terminal cytoplasmic domain, a 20 amino acid residue hydrophobic signal peptide/membrane anchor domain, and a large catalytic COOH-terminal domain exposed on the exoplasmic side of the membrane. To study the role of the cytosolic domain in anchoring NEP in the plasma membrane, we constructed two mutants in which this cytosolic domain was deleted. In the first mutant (NEPΔcyto), a Glu residue was present in NH2-terminus, while a Lys residue was substituted at the same posit

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Kalchenko, Vitaly, Olga Kalchenko, and Sergiy Cherenok. "Complexation of Calix[4]arene bis-Hydroxymethylenediphosphonic Acid with Amino acids. Binding Constants Determination by RP HPLC Method." French-Ukrainian Journal of Chemistry 3, no. 2 (2015): 93–100. http://dx.doi.org/10.17721/fujcv3i2p93-100.

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Host-Guest complexation of calixarene-bis-hydroxymethylenediphosphonic acid with 17 amino acids in water solution had been studied by the RP HPLC and molecular modelling methods. It had been shown the binding constants of the complexes are depended on the nature of the amino acid residue, log P and pKa of the acids. The complexation is mainly determined by the electrostatic interactions between the positively charged nitrogen atom of the amino acid and the negatively charged oxygen atom of phosphonic acid residue of the calixarene, the Host-Guest p-p, СН-p and solvophobic interactions.

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Fernandez de la Mora, J. "Electrospray ionization of large multiply charged species proceeds via Dole’s charged residue mechanism." Analytica Chimica Acta 406, no. 1 (2000): 93–104. http://dx.doi.org/10.1016/s0003-2670(99)00601-7.

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Mayol, Eduardo, Mercedes Campillo, Arnau Cordomí, and Mireia Olivella. "Inter-residue interactions in alpha-helical transmembrane proteins." Bioinformatics 35, no. 15 (2018): 2578–84. http://dx.doi.org/10.1093/bioinformatics/bty978.

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Abstract Motivation The number of available membrane protein structures has markedly increased in the last years and, in parallel, the reliability of the methods to detect transmembrane (TM) segments. In the present report, we characterized inter-residue interactions in α-helical membrane proteins using a dataset of 3462 TM helices from 430 proteins. This is by far the largest analysis published to date. Results Our analysis of residue–residue interactions in TM segments of membrane proteins shows that almost all interactions involve aliphatic residues and Phe. There is lack of polar–polar, po

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Mahdavi, Atiyeh, Reza H. Sajedi, Saman Hosseinkhani, and Majid Taghdir. "Hyperactive Arg39Lys mutated mnemiopsin: implication of positively charged residue in chromophore binding cavity." Photochemical & Photobiological Sciences 14, no. 4 (2015): 792–800. http://dx.doi.org/10.1039/c4pp00191e.

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Mnemiopsin, a Ca<sup>2+</sup>-regulated photoprotein isolated fromMnemiopsis leidyi, belongs to the family of ctenophore photoproteins. While there are no charged amino acid residues in the coelenterazine binding cavity of cnidarian photoproteins, ctenophore photoproteins have a positively charged residue (Arg) in this region.

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Miniero, Daniela Valeria, Magnus Monné, Maria Antonietta Di Noia, Luigi Palmieri, and Ferdinando Palmieri. "Evidence for Non-Essential Salt Bridges in the M-Gates of Mitochondrial Carrier Proteins." International Journal of Molecular Sciences 23, no. 9 (2022): 5060. http://dx.doi.org/10.3390/ijms23095060.

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Mitochondrial carriers, which transport metabolites, nucleotides, and cofactors across the mitochondrial inner membrane, have six transmembrane α-helices enclosing a translocation pore with a central substrate binding site whose access is controlled by a cytoplasmic and a matrix gate (M-gate). The salt bridges formed by the three PX[DE]XX[RK] motifs located on the odd-numbered transmembrane α-helices greatly contribute to closing the M-gate. We have measured the transport rates of cysteine mutants of the charged residue positions in the PX[DE]XX[RK] motifs of the bovine oxoglutarate carrier, t

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He, Yuxian, Shuwen Liu, Jingjing Li, et al. "Conserved Salt Bridge between the N- and C-Terminal Heptad Repeat Regions of the Human Immunodeficiency Virus Type 1 gp41 Core Structure Is Critical for Virus Entry and Inhibition." Journal of Virology 82, no. 22 (2008): 11129–39. http://dx.doi.org/10.1128/jvi.01060-08.

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ABSTRACT The fusogenic human immunodeficiency virus type 1 (HIV-1) gp41 core structure is a stable six-helix bundle formed by its N- and C-terminal heptad repeat sequences. Notably, the negatively charged residue Asp632 located at the pocket-binding motif in the C-terminal heptad repeat interacts with the positively charged residue Lys574 in the pocket formation region of the N-terminal heptad repeat to form a salt bridge. We previously demonstrated that the residue Lys574 plays an essential role in six-helix bundle formation and virus infectivity and is a key determinant of the target for ant

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Li, Yuan, Xing Li, Matthew Stremlau, Mark Lee та Joseph Sodroski. "Removal of Arginine 332 Allows Human TRIM5α To Bind Human Immunodeficiency Virus Capsids and To Restrict Infection". Journal of Virology 80, № 14 (2006): 6738–44. http://dx.doi.org/10.1128/jvi.00270-06.

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ABSTRACT Human TRIM5α (TRIM5αhu) only modestly inhibits human immunodeficiency virus type 1 (HIV-1) and does not inhibit simian immunodeficiency virus (SIVmac). Alteration of arginine 332 in the TRIM5αhu B30.2 domain to proline, the residue found in rhesus monkey TRIM5α, has been shown to create a potent restricting factor for both HIV-1 and SIVmac. Here we demonstrate that the potentiation of HIV-1 inhibition results from the removal of a positively charged residue at position 332 of TRIM5αhu. The increase in restricting activity correlated with an increase in the ability of TRIM5αhu mutants

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Thèses sur le sujet "Charged-residue"

TEDESCHI, GIULIA. "Effect of electrostatic charges on aggregation and conformation of intrinsically disordered proteins." Doctoral thesis, Università degli Studi di Milano-Bicocca, 2018. http://hdl.handle.net/10281/198946.

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Intrinsecamente disordinata” viene definita una proteina nativa priva di struttura secondaria o terziaria, non esposta ad agenti denaturanti. Le proteine con queste caratteristiche sono indicate come IDP/IDR, e rappresentano una ampia porzione del proteoma di tutti gli esseri viventi. Le IDP sono coinvolte in molte funzioni fisiologiche e patologiche, come la formazione di organuli cellulari privi di membrane e nei processi di fibrillazione associate ad amiloidosi. Entrambi questi fenomeni appaiono sempre più associati alla capacità delle IDP di formare interazioni intermolecolari. Stati patol

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Lee, Logan Eric Elmer. "A charged residue in the turret or outer pore mouth of hKv1.5 alters macroscopic current kinetics." Thesis, 2005. http://hdl.handle.net/2429/16621.

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Using hKvl.5 expressed in HEK-293 cells the roles of R487 and H463 in the processes of permeation and inactivation were studied. Mutation of the arginine at position 487 to a valine (R487V) alters the I-Vrelationship through Kvl.5 such that the normalized current at positive voltages, in symmetrical 140 mM K+ , is less than at negative voltages. The magnitude of current reduction at positive voltages was unaffected by extracellular acidification suggesting that the protonation state of H463 does not effect the rate of K + exit or entry at the outer pore mouth. Using an Eyring-rate model

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Chapitres de livres sur le sujet "Charged-residue"

Tran, Thanh Hoa, and Giuseppe Legname. "Replacement of Residue H95 with Charged Amino Acids in the Prion Protein Decreases Prion Conversion Propensity." In IFMBE Proceedings. Springer Singapore, 2019. http://dx.doi.org/10.1007/978-981-13-5859-3_46.

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Schwarz, Schwarz. "Saccharomyces cerevisiae Mdjlp." In Guidebook to Molecular Chaperones and Protein-Folding Catalysts. Oxford University PressOxford, 1997. http://dx.doi.org/10.1093/oso/9780198599494.003.0040.

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Abstract The nuclear gene for mitochondrial DnaJ (MDJ1) was identified during the sequence analysis of chromosome VI (GenBank accession number D50617x53). The MDJ1 open reading frame encodes a polypeptide of 511 residues (Rowley et al., 1994) that possesses all the features characteristic of a DnaJ protein: (i) an N-terminal J-domain of about 70 amino acid residues; (ii) a 60-residue segment rich in glycine, phenylalanine; (iii) four cysteine-containing repeats with the sequence motif: CXXCXGXG (with X being predominantly a polar or charged residue);, (iv) a C-terminal domain with little seque

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Bandyopadhyay, Amal Kumar, Sahini Banerjee, and Somnath Das. "Discovery of rim region between core and surface of proteins." In Life as Basic Science: An Overview and Prospects for the Future [Volume: 3], 3rd ed. International Academic Publishing House (IAPH), 2024. https://doi.org/10.52756/lbsopf.2024.e03.003.

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The crystal structure reveals the complexities of protein component organization from the core to the surface. In general, the existence of core and surface in protein structure is well known. Here, we raise the question of how this core and surface, respectively, end and begin. To further understand the concerns, we did a comprehensive investigation on high-resolution structures of three protein families [ADH (Alcohol Dehydrogenase), Glyceraldehyde 3-Phosphate Dehydrogenase (GDH), and Malate Dehydrogenase (MDH)] from various domains of life utilizing authentic methods. The results demonstrate

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Actes de conférences sur le sujet "Charged-residue"

Pietri, S. "Coincidence Measurement of Residue, Neutrons, and Light Charged Particles in a Spallation Experiment." In INTERNATIONAL CONFERENCE ON NUCLEAR DATA FOR SCIENCE AND TECHNOLOGY. AIP, 2005. http://dx.doi.org/10.1063/1.1945124.

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Hafer, Richard F., Oliver D. Patterson, Cathy Gow, Derek McKindles, Brian Yueh-Ling Hsieh, and Xiaohu Tang. "In-Line Electron Beam Inspection of High Aspect-Ratio RMG FINFET Gate." In ISTFA 2017. ASM International, 2017. http://dx.doi.org/10.31399/asm.cp.istfa2017p0380.

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Abstract For a recent replacement metal gate (RMG) technology using a SOI substrate, residue from the dummy gate formed a defect that affected the RMG formation. In this FINFET technology, the high aspect ratio of the gate makes removing the dummy gate very difficult. Residue is left behind, especially in multi-fin structures. This residue was poorly detected by existing Broad-Band-Plasma inspection and thus required Electron Beam Inspection. However, this physical inspection is challenging due to high aspect ratio of the gate and an insulating wafer surface. The defect was verified using TEM,

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Handa, M., K. Titani, K. Takio, and Z. M. Ruggeri. "CHARACTERIZATION OF THE VON WILLEBRAND FACTOR-BINDING DOMAIN OF PLATELET MEMBRANE GLYCOPROTEIN Ib." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1642925.

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We have previously obtained immunochemical evidence that the von Willebrand factor (vWF)-binding domain of the platelet membrane glycoprotein (GP) Ib is located near the amino terminus of the a subunit (Journal of Biological Chemistry 261: 12579-12585, 1986). We have now determined the complete amino acid sequence of the 45 kDa tryptic fragment of glycocalicin that contains this domain. Purified glycocalicin was subjected to limited digestion with trypsin and the proteolytic fragments were separated by size-exclusion high-pressure liquid chromatography. Two fragments of 45 kDa and 84 kDa, resp

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Yang, Xiaojian, and Guoming G. Zhu. "SI and HCCI Combustion Mode Transition Control of a Multi-Cylinder HCCI Capable SI Engine via Iterative Learning." In ASME 2011 Dynamic Systems and Control Conference and Bath/ASME Symposium on Fluid Power and Motion Control. ASMEDC, 2011. http://dx.doi.org/10.1115/dscc2011-6020.

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The combustion mode transition between spark ignition (SI) and homogeneously charged compression ignition (HCCI) combustions of an internal combustion (IC) engine is challenging due to the distinct engine operational parameters over these two combustion modes and the cycle-to-cycle residue gas dynamics of the HCCI combustion. The control problem becomes even more complicated when multi-cylinder operation is involved. This paper studies the combustion mode transition problem of a multi-cylinder IC engine with dual-stage valve lifts and electrical variable valve timing systems. A control oriente

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Yang, Xiaojian, Guoming G. Zhu, and Zongxuan Sun. "A Control Oriented SI and HCCI Hybrid Combustion Model for Internal Combustion Engines." In ASME 2010 Dynamic Systems and Control Conference. ASMEDC, 2010. http://dx.doi.org/10.1115/dscc2010-4042.

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The combustion mode transition between SI (spark ignited) and HCCI (Homogeneously Charged Compression Ignition) of an IC (Internal Combustion) engine is challenge due to the thermo inertia of residue gas; and model-based control becomes a necessity. This paper presents a control oriented two-zone model to describe the hybrid combustion that starts with SI combustion and ends with HCCI combustion. The gas respiration dynamics were modeled using mean-value approach and the combustion process was modeled using crank resolved method. The developed model was validated in an HIL (Hardware-In-the-Loo

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JAWAD, Israa, Adian Abd Alrazak DAKL, and Hussein Jabar JASIM. "CHARACTERIZATION, MECHANISM OF ACTION, SOURCES TYPES AND USES OF THE ANTIMICROBIAL PEPTIDES IN DOMESTIC ANIMALS, REVIEW." In VII. INTERNATIONAL SCIENTIFIC CONGRESSOF PURE,APPLIEDANDTECHNOLOGICAL SCIENCES. Rimar Academy, 2023. http://dx.doi.org/10.47832/minarcongress7-13.

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This review aimed to identify the general characteristics of , mechanism of action, types and uses of antimicrobial peptides in animals, antimicrobial peptides were lass of small peptides that widely exist naturally, they varied greatly in structure, composition are found in the animal's species, and were standard structural features, twenty to sixty residue long, cationic and amphipathic peptides, have a positive charge that interacted with negatively charged molecules on the bacterial cell surfaces, a have an expansive field of inhibitory effects and were made as the first line of protection

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Avramenko, Valentin, Svetlana Bratskaya, Dmitry Marinin, Anatoliy Terzi, and Mariya Yarmolyuk. "Pilot Test of Precipitation Setup for Dust Supressor and Transuranic Elements Removal From Wastewaters of Chernobyl Nuclear Power Plant." In ASME 2011 14th International Conference on Environmental Remediation and Radioactive Waste Management. ASMEDC, 2011. http://dx.doi.org/10.1115/icem2011-59256.

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In this paper we report the results of pilot tests of flocculation/precipitation setup for dust-suppressor (DS) and transuranic elements (TUE) removal from wastewaters of Chernobyl Nuclear Power Plant (ChNPP), including those of the Object “Shelter”. Tests were performed on the pilot unit (PU), which included service tank, precipitation tank, and accumulation tank, 300 dm3 each, connected with pipelines with dosing and pumping equipment, and throttle valves providing controlled dosing, mixing, precipitation and mechanical filtration of radioactive wastewaters under different conditions. The re

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Rapports d'organisations sur le sujet "Charged-residue"

Gurevitz, Michael, Michael Adams, and Eliahu Zlotkin. Insect Specific Alpha Neurotoxins from Scorpion Venoms: Mode of Action and Structure-Function Relationships. United States Department of Agriculture, 1996. http://dx.doi.org/10.32747/1996.7613029.bard.

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This study was motivated by the need to develop new means and approaches to the design of future, environmentally-safe, insecticides. Utilization of anti-insect selective toxins from scorpion venoms and clarification of the molecular basis for their specificity, are a major focus in this project and may have an applicative value. Our study concentrated on the highly insecticidal toxin, LqhaIT, and was devoted to: (I) Characterization of the neuropharmacological and electrophysiological features of this toxin. (II) Establishment of a genetic system for studying structure/activity relationships

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Barefoot, Susan F., Bonita A. Glatz, Nathan Gollop, and Thomas A. Hughes. Bacteriocin Markers for Propionibacteria Gene Transfer Systems. United States Department of Agriculture, 2000. http://dx.doi.org/10.32747/2000.7573993.bard.

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The antibotulinal baceriocins, propionicin PLG-1 and jenseniin G., were the first to be identified, purified and characterized for the dairy propionibaceria and are produced by Propionibacterium thoenii P127 and P. thoenii/jensenii P126, respectively. Objectives of this project were to (a) produce polyclonal antibodies for detection, comparison and monitoring of propionicin PLG-1; (b) identify, clone and characterize the propionicin PLG-1 (plg-1) and jenseniin G (jnG) genes; and (3) develop gene transfer systems for dairy propionibacteria using them as models. Polyclonal antibodies for detecti

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