Articles de revues : « Copper accumulation »

1

Tsekova, K. V., P. G. Marinov et A. N. Tzekova. « Copper accumulation byAspergillus awamori ». Folia Microbiologica 45, n^o 3 (juin 2000) : 217–20. http://dx.doi.org/10.1007/bf02908947.

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Thornburg, Larry P. « A Perspective on Copper and Liver Disease in the Dog ». Journal of Veterinary Diagnostic Investigation 12, n^o 2 (mars 2000) : 101–10. http://dx.doi.org/10.1177/104063870001200201.

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Copper is a ubiquitous trace metal necessary for normal function of a variety of cellular proteins. Intracellular copper metabolism is complex, and only a few of the proteins/genes involved are known. Copper deficiency does not appear to be a clinical problem in dogs. Excess copper accumulation in the liver as a cause of hepatitis and cirrhosis was first demonstrated among Bedlington terriers. Subsequently, copper accumulation in the liver has been shown to occur in several other breeds of dogs. Excess hepatic copper has been found in dogs with normal liver histology, dogs with hepatitis, and dogs with end stage cirrhosis. Evidence is accumulating that copper is a cause of liver disease in breeds of dogs other than Bedlington terriers. Moreover, as more data are accumulated, the copper storage disease appears to have characteristics that are very similar among all of the affected breeds.

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Scheiber, Ivo F., Julian F. B. Mercer et Ralf Dringen. « Copper accumulation by cultured astrocytes ». Neurochemistry International 56, n^o 3 (février 2010) : 451–60. http://dx.doi.org/10.1016/j.neuint.2009.12.002.

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Vulpe, Constantina Bianca, Mariana Adina Matica, Renata Kovačević, Daniela Dascalu, Zoran Stevanovic, Adriana Isvoran, Vasile Ostafe et Gheorghița Menghiu. « Copper Accumulation Efficiency in Different Recombinant Microorganism Strains Available for Bioremediation of Heavy Metal-Polluted Waters ». International Journal of Molecular Sciences 24, n^o 8 (20 avril 2023) : 7575. http://dx.doi.org/10.3390/ijms24087575.

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The aim of this research was to investigate the bioremediation conditions of copper in synthetic water. In the present study, copper ions accumulation efficiency was determined using various genetically modified strains of Saccharomyces cerevisiae (EBY100, INVSc1, BJ5465, and GRF18), Pichia pastoris (X-33, KM71H), Escherichia coli (XL10 Gold, DH5α, and six types of BL21 (DE3)), and Escherichia coli BL21 (DE3) OverExpress expressing two different peroxidases. Viability tests of yeast and bacterial strains showed that bacteria are viable at copper concentrations up to 2.5 mM and yeasts up to 10 mM. Optical emission spectrometry with inductively coupled plasma analysis showed that the tolerance of bacterial strains on media containing 1 mM copper was lower than the tolerance of yeast strains at the same copper concentration. The E. coli BL21 RIL strain had the best copper accumulation efficiency (4.79 mg/L of culture normalized at an optical density of 1.00), which was 1250 times more efficient than the control strain. The yeast strain S. cerevisiae BJ5465 was the most efficient in copper accumulation out of a total of six yeast strains used, accumulating over 400 times more than the negative control strain. In addition, E. coli cells that internally expressed recombinant peroxidase from Thermobifida fusca were able to accumulate 400-fold more copper than cells that produced periplasmic recombinant peroxidases.

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Herd, S. M., J. Camakaris, R. Christofferson, P. Wookey et D. M. Danks. « Uptake and efflux of copper-64 in Menkes'-disease and normal continuous lymphoid cell lines ». Biochemical Journal 247, n^o 2 (15 octobre 1987) : 341–47. http://dx.doi.org/10.1042/bj2470341.

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The accumulation of copper over 2 h by normal lymphoid cells and those from Menkes'-disease patients (Menkes' cells) was found to be biphasic, with an initial phase of rapid uptake, an approach to steady state at around 40-60 min, followed by a further accumulation phase. The accumulation of copper was not diminished by the addition of a variety of metabolic inhibitors, suggesting that copper uptake is not an active process. The presence of carbonyl cyanide m-chlorophenylhydrazone in the culture medium stimulated the uptake and accumulation of copper in both normal and Menkes' cells to the same absolute level. This effect appeared to be specific for copper, since the accumulation of Zn and Cd was unaffected. Menkes' cells did not differ from normal in their initial rate of copper uptake. Analysis of the uptake curve suggested that the membrane transport of copper involves both passive and facilitated diffusion. Initial rate of efflux from the cells was approximated by two methods. Menkes' cells did not appear to be affected in this function. It seems likely that the basic defect in Menkes' disease involves a step in intracellular copper transport rather than the membrane transport of copper.

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Richard, Julianne N., Jennifer E. Graham, Sharon A. Center, Michael M. Garner et Drury R. Reavill. « Hepatic copper accumulates in ferrets with and without hepatobiliary disease ». American Journal of Veterinary Research 83, n^o 4 (1 avril 2022) : 371–80. http://dx.doi.org/10.2460/ajvr.21.08.0131.

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Abstract OBJECTIVE To determine hepatic copper concentrations and zonal distribution in ferrets with and without hepatobiliary disease, validate rhodanine-based qualitative copper scoring and digital copper quantification in ferret hepatic samples, and ascertain whether clinical features predicted copper accumulation. ANIMALS 34 ferrets, including 7 with necroinflammatory disease, 5 with hepatocellular carcinoma, 13 with non-necroinflammatory disease, and 9 with no hepatobiliary disease. PROCEDURES Rhodanine-based digital copper quantification was validated by use of liver dually measured by atomic absorption spectroscopy and digital scanning (R2 = 0.98). Clinical features and hepatic copper scores and concentrations (dry weight liver) were compared between groups. Zonal copper distribution was determined. RESULTS Hepatic copper concentration was strongly correlated with copper scores (ρ = 0.88). Ferrets with hepatobiliary disease were significantly older and had significantly higher serum alkaline phosphatase and γ-glutamyltransferase activities and creatinine concentrations. Centrilobular copper accumulated in 23 of 34 (64%) ferrets with (n = 15) and without (8) hepatobiliary disease. Median copper concentrations were not significantly different between ferrets with and without hepatobiliary disease but were significantly higher within neoplastic hepatic tissue in ferrets with hepatocellular carcinoma. Hepatic copper concentrations exceeded feline (> 180 µg/g) and canine (> 400 µg/g) reference limits in 19 and 9 ferrets, respectively. Hepatic copper > 1,000 µg/g occurred in 5 ferrets with and 2 without hepatobiliary disease. Clinical features did not predict copper accumulation. CLINICAL RELEVANCE Rhodanine-based digital copper quantification and qualitative copper scoring discerned liver copper accumulation in ferrets. Ferrets with and without hepatobiliary disease displayed a propensity for centrilobular hepatic copper accumulation of uncertain clinical importance. Clinical and clinicopathologic features could not exclusively implicate pathologic copper accumulation.

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Haywood, S., H. C. Rutgers et M. K. Christian. « Hepatitis and Copper Accumulation in Skye Terriers ». Veterinary Pathology 25, n^o 6 (novembre 1988) : 408–14. http://dx.doi.org/10.1177/030098588802500602.

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Livers of nine related Skye terriers with liver disease were evaluated for histological changes and copper content. Lesions ranged from hepatocellular degeneration and necrosis (zone 3) with intracanalicular cholestasis and mild inflammation, to chronic hepatitis with cholangioplasia and cirrhosis. Excess copper (801–2,257 μg/g) was related to the severity of cholestasis. Skye terrier hepatitis is a distinct disease entity and may be derived from a disorder of intracellular bile metabolism culminating in disturbed bile secretion and the accumulation of copper.

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Toselli, M., P. Schiatti, D. Ara, A. Bertacchini et M. Quartieri. « The accumulation of copper in soils of the Italian region Emilia-Romagna ». Plant, Soil and Environment 55, No. 2 (24 février 2009) : 74–79. http://dx.doi.org/10.17221/317-pse.

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The investigation was carried out in 2005, on 30 plots chosen in the Central-Eastern part of the Emilia Romagna region, and cultivated with pear, grapevine and vegetable crops under the organic management system. For each crop, 5 plots with a level of calcium carbonate > 10% and 5 plots with a level of calcium carbonate < 3% were selected. For pear and vine, soil analyses were performed at the depths of 0–20 cm and 20–50 cm, for vegetable at the depth of 0–50 cm. Organic matter content was higher in pear-cultivated plots, followed by grapevine and vegetable crops. Copper application rate, from 1998 to 2004, was higher in pear and grapevine than in vegetable plots. Soil total and DTPA-extractable Cu were higher in pear and grapevine than in vegetable-cultivated plots. Soil DTPA-extractable Cu concentration was higher in the upper horizon than at 20–50 cm soil depth. The increase of total Cu in pear and vine-cultivated plots was combined with the increase of soil inactive Cu.

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Mitani, Tomoyo, et Dragoslav M. Misic. « Copper accumulation byPenicilliumsp. isolated from soil ». Soil Science and Plant Nutrition 37, n^o 2 (juin 1991) : 347–49. http://dx.doi.org/10.1080/00380768.1991.10415045.

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Tsekova, Kolishka, Danka Galabova et Kristina Todorova. « Copper Accumulation and Phosphatase Activities of Aspergillus and Rhizopus ». Zeitschrift für Naturforschung C 55, n^o 9-10 (1 octobre 2000) : 708–12. http://dx.doi.org/10.1515/znc-2000-9-1007.

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Abstract Copper accumulation and phosphatase activities of three Aspergillus species resistant to copper were compared to three copper-sensitive Rhizopus species. High level of acid phosphatases and decreased Cu2+-uptake were found with resistant in contrast to sensitive strains. The presence of copper(II) ions in the medium increased the production of acid phosphatases in the resistant A. niger and decreased their activity in the sensitive R. delemar. Copper ions inhibited the activity of A. niger cellular acid phosphatase with a Ki of 8.9x10-4 ᴍ and slightly activated the R. delemar enzyme.

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Waldrop, G. L., F. A. Palida, M. Hadi, P. A. Lonergan et M. J. Ettinger. « Effect of albumin on net copper accumulation by fibroblasts and hepatocytes ». American Journal of Physiology-Gastrointestinal and Liver Physiology 259, n^o 2 (1 août 1990) : G219—G225. http://dx.doi.org/10.1152/ajpgi.1990.259.2.g219.

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The liver accumulates copper rapidly and preferentially from plasma. The effects of albumin on net copper accumulation by fibroblasts and hepatocytes were compared to determine whether preferential uptake involves hepatocyte-specific sequestering of copper. Although albumin inhibits the initial rates (30 s) of copper transport by fibroblasts and hepatocytes similarly, the effects of albumin on net copper accumulation (4 h) by these cell types were strikingly different. Fibroblasts accumulate only approximately 15% as much copper when equimolar albumin is present as from albumin-free media; hepatocytes accumulate about the same amount of copper with or without extracellular albumin present. Copper efflux data show that the special capacity of hepatocytes to accumulate copper in the presence of extracellular albumin is due to greater copper retention by hepatocytes than fibroblasts. The ability of hepatocytes to accumulate copper does not seem to be due to albumin-receptor-mediated uptake, since albumin was not co-transported with copper. The data are consistent with an equilibrium model of copper accumulation in which intracellular and extracellular copper are in equilibrium with intracellular and extracellular ligands. A high-affinity, copper-binding fraction that was previously identified in cytosols from hepatocytes was low or absent in fibroblasts. This may contain a liver-specific protein(s) that helps hepatocytes sequester and retain copper from albumin or serum-containing media. Irrespective of the exact species involved, the data are consistent with rapid, preferential copper uptake by the liver being due in part to a liver-specific, intracellular copper-binding protein(s) with a high binding affinity for copper.

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Freitas, F., S. Lunardi, L. B. Souza, J. S. C. von der Osten, R. Arruda, R. L. T. Andrade et L. D. Battirola. « Accumulation of copper by the aquatic macrophyte Salvinia biloba Raddi (Salviniaceae) ». Brazilian Journal of Biology 78, n^o 1 (10 juillet 2017) : 133–39. http://dx.doi.org/10.1590/1519-6984.166377.

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Abstract Aquatic macrophytes have properties and mechanisms which are useful for the removal of substances in solution, commonly used in phytoremediation processes in aquatic environments. This study evaluated the performance of copper (Cu) accumulation by Salvinia biloba Raddi (Salviniaceae) in different metal concentrations (1, 3 and 5 µg mL-1), as well as the control treatment, measured at intervals of 0, 7 and 14 days under laboratory conditions, with control as to pH and luminosity. After the experiment, the S. biloba biomass was washed, kiln dried, crushed and subjected to the process of digestion, and subsequently the accumulated copper content was determined by atomic absorption spectroscopy. The results showed that S. biloba is apt at accumulating copper, varying significantly between different treatments and days of exposure to the contaminant, as well as its interaction (treatment × days). The highest accumulation values were observed in treatment with 5 µg mL-1, which at 14 days, with 11,861 µg g-1 of copper. We observed symptoms of toxicity and mortality in plants, probably indicating the effect of copper on the species when at high levels. Salvinia biloba is an efficient species in the removal of copper in solution, its recommendation as a remediating agent in aquatic ecosystems being possible.

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Ruta, Lavinia L., Melania A. Banu, Aurora D. Neagoe, Ralph Kissen, Atle M. Bones et Ileana C. Farcasanu. « Accumulation of Ag(I) by Saccharomyces cerevisiae Cells Expressing Plant Metallothioneins ». Cells 7, n^o 12 (11 décembre 2018) : 266. http://dx.doi.org/10.3390/cells7120266.

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The various applications of Ag(I) generated the necessity to obtain Ag(I)-accumulating organisms for the removal of surplus Ag(I) from contaminated sites or for the concentration of Ag(I) from Ag(I)-poor environments. In this study we obtained Ag(I)-accumulating cells by expressing plant metallothioneins (MTs) in the model Saccharomyces cerevisiae. The cDNAs of seven Arabidopsis thaliana MTs (AtMT1a, AtMT1c, AtMT2a, AtMT2b, AtMT3, AtMT4a and AtMT4b) and four Noccaea caerulescens MTs (NcMT1, NcMT2a, NcMT2b and NcMT3) fused to myrGFP displaying an N-terminal myristoylation sequence for plasma membrane targeting were expressed in S. cerevisiae and checked for Ag(I)-related phenotype. The transgenic yeast cells were grown in copper-deficient media to ensure the expression of the plasma membrane high-affinity Cu(I) transporter Ctr1, and also to elude the copper-related inhibition of Ag(I) transport into the cell. All plant MTs expressed in S. cerevisiae conferred Ag(I) tolerance to the yeast cells. Among them, myrGFP-NcMT3 afforded Ag(I) accumulation under high concentration (10–50 μM), while myrGFP-AtMT1a conferred increased accumulation capacity under low (1 μM) or even trace Ag(I) (0.02–0.05 μM). The ability to tolerate high concentrations of Ag(I) coupled with accumulative characteristics and robust growth showed by some of the transgenic yeasts highlighted the potential of these strains for biotechnology applications.

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Chao, Chun Yan, et Deng Jun Ma. « The Effects of Selenium on Toxicity of Copper on Rape ». Advanced Materials Research 610-613 (décembre 2012) : 288–91. http://dx.doi.org/10.4028/www.scientific.net/amr.610-613.288.

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To research the effect of how Se alleviate the harm brought by copper, we investigated the root length, stem, leaves, aberration by Cu colza in copper and Se-Cu compounds. The experimental simulation of sewage irrigation methods, the general consumption of rapeseed selected as experimental material, using the method of comparison, were dealing with a single copper, different concentrations selenium and copper concentrations were compared with experimental treatment. The Experiments were divided into three groups of treatment, respectively with a single copper, low concentrations selenium and copper and high concentrations of selenium and copper processing of rape. The focus is research the effect of selenium on the toxicity of copper. The result shows that the law of heavy metals like copper accumulation in the soil as well as in the migration and accumulation in rape and the law of metallic copper in the role of selenium in the soil accumulation as well as in the migration and accumulation in rape. The copper in the soil and rape are determinated by AAS. The results show that Selenium effectively alleviate the toxicity of copper on rape, and the ability of ease is high concentrations of selenium intensity than low concentrations of selenium.

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Goto, Norika, Hirokazu Hara, Mao Kondo, Naomi Yasuda, Tetsuro Kamiya, Kensuke Okuda et Tetsuo Adachi. « Hydrogen sulfide increases copper-dependent neurotoxicity via intracellular copper accumulation ». Metallomics 12, n^o 6 (2020) : 868–75. http://dx.doi.org/10.1039/d0mt00015a.

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Kruckeberg, A. L., et L. Wu. « Copper tolerance and copper accumulation of herbaceous plants colonizing inactive California copper mines ». Ecotoxicology and Environmental Safety 23, n^o 3 (juin 1992) : 307–19. http://dx.doi.org/10.1016/0147-6513(92)90080-m.

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Irawati, Wahyu. « ISOLASI DAN KARAKTERISASI BAKTERI RESISTEN TEMBAGA DARI PANTAI TIMUR SURABAYA ». BIOLINK (Jurnal Biologi Lingkungan Industri Kesehatan) 6, n^o 2 (17 décembre 2019) : 95–105. http://dx.doi.org/10.31289/biolink.v6i2.2558.

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Copper pollution in the East Coast of Surabaya is one of the serious cases of heavy metal pollution in Indonesia. Pollution at this location has been proven to result in fish deaths and brain damage of local residents because they consume too much copper-contaminated fish. Copper bioremediation using indigen bacteria isolated from polluted environments is a promising solution to overcome copper pollution problems. Bacteria that commonly live in polluted environments can be isolated and used as copper bioremediation agents. This study aims to do: 1) isolation and characterization of copper resistant bacteria from the East Coast of Surabaya, 2) resistance testing of bacterial isolates, and 3) copper accumulation and biosorbtion tests of bacterial isolates. The bacterial isolates were characterized by the morphology of the colonies and their cells based on Gram staining. Resistance testing is done by determining Minimum Inhibitory Concentration/MIC. The accumulation test is carried out by separating the cell and growth medium, then each of them is distructed using HNO3. The results of isolation and characterization obtained six isolates of copper-resistant bacteria, namely PmbC1, PmbC2, PmbC3, PmbC4, PmbC5, and PmbC6 with MIC = 3 mM - 5mM CuSO4. PmbC4 isolate is the most resistant bacteria with the MIC of 5 mM and is capable of accumulating copper of 6.25 mg per gram of cell dry weight and biosorbtion of 92.17%.

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Mužek, Mario Nikola, Dario Omanović, Azra Đulović, Franko Burčul, Sandra Svilović et Ivica Blažević. « The Garden Candytuft (Iberis umbellata L.) : At the Crossroad of Copper Accumulation and Glucosinolates ». Processes 8, n^o 9 (8 septembre 2020) : 1116. http://dx.doi.org/10.3390/pr8091116.

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The copper accumulation ability and its impact on the glucosinolate content of the garden candytuft were studied. Different copper sources (adsorbents or solution) were used. Generally, the seedlings copper uptake from the adsorbents was in the amount needed for its growth and development with the beneficial or no impact on the glucosinolate content. The lowest copper concentration was detected in the total seedlings biomass which grew in the humus with the addition of Cu-exchanged zeolite NaX (27.88 μg g−1 DW) having glucosinolate content of 9757.81 µg g−1 DW (23.86 µmol g−1 DW). The highest copper concentration among all the garden candytuft samples was detected in the seedlings watered with CuSO4∙5H2O solution (514.63 μg g−1 DW) with a sharp decrease of the glucosinolate content 3103.33 µg g−1 DW (7.59 µmol g−1 DW). Based on the results obtained, the garden candytuft can be considered as a copper accumulator plant.

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Trush, Kristina, Natália Handzušová et Peter Paľove-Balang. « Changes in Isoflavonoid and Flavonoid Content in Soybean Leaves Affected by UV-B or Copper ». Agriculture (Pol'nohospodárstvo) 69, n^o 3 (1 octobre 2023) : 140–48. http://dx.doi.org/10.2478/agri-2023-0012.

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Abstract The production of antioxidant flavonoids is an important aspect of plant defence mechanisms under stress conditions. The hypothesis of increased antioxidant flavonol quercetin and isoflavone content in soybean leaves subjected to UV-B irradiation and copper toxicity was proposed, and their accumulation was investigated using HPLC analysis. However, the accumulation of antioxidant quercetin was not induced by either UV-B irradiation or copper treatment. UV-B irradiation strongly induced isoflavone aglycones, mainly genistein, daidzein, formononetin, and to a lesser extent, biochanin A. Isoflavone glycosides, genistin and daidzin were also induced. Copper treatment, whether applied to leaves or root media, resulted in the accumulation of genistin and its malonylated form, whereas aglycones were only slightly affected. The higher accumulation of genistein over daidzein after copper treatment could be related to the higher chelating ability of genistein and its glycosides. Soybean isoflavones, which possess antioxidant and chelating properties, rather than flavonols, play a crucial role in the plant‘s defence mechanisms against both UV-B irradiation and copper toxicity.

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Li, Hongxia, Xue Gao, Shaofeng Zhao, He Tian et Qinfang Wei. « New Targets for Treating Cancer : Copper Death and Copper Accumulation in Cancer Cells ». International Journal of Biology and Life Sciences 8, n^o 1 (22 novembre 2024) : 41–46. http://dx.doi.org/10.54097/9v67nn75.

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The accumulation of copper ions plays an important role in the apoptosis of cancer cells. Definition of copper death: Copper death (Cuproptosis) is a new form of programmed cell death caused by excessive copper. Copper ions can participate in multiple cellular signaling pathways and promote the growth and division of cancer cells. Copper ions are involved in angiogenesis, providing nutrients and oxygen for cancer cells and supporting the growth and spread of tumors. Together, they promote the progression of cellular inflammation. Copper ions are related to tumor metastasis and play a certain role in the migration and invasion of cancer cells.

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Ozuah, Philip O. « Wilson Disease ». Pediatrics In Review 17, n^o 12 (1 décembre 1996) : 448. http://dx.doi.org/10.1542/pir.17.12.448.

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Wilson disease (hepatolenticular degeneration) is an autosomal recessive, inherited disorder of copper metabolism resulting in excessive accumulation of copper in the liver, brain, and other organs of the body. The manifestations of the disease are related directly to this accumulation of copper. Copper homeostasis normally is a product of the balance between intestinal absorption of dietary copper and hepatic biliary excretion of excess copper. In Wilson disease, incorporation of hepatic copper into ceruloplasmin is defective and excretion of copper in the bile is reduced. A low level of ceruloplasmin, which until a few years ago was erroneously considered to be the basis for the disease, is a consequence of the underlying metabolic defect.

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Andreeva, Irina, Valeria Gabechaya, Dmitriy Morev, Miljan Samardžić, Zoran Galić et Alexis Yaroslavtsev. « How Landscapes and History Shape Copper in Vineyard Soils : Example of Fruška Gora Region, Serbia ». Land 14, n^o 1 (6 janvier 2025) : 96. https://doi.org/10.3390/land14010096.

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Vineyards are distinctive agroecosystems heavily influenced by local natural factors and traditional management practices, with significant implications for the quality and quantity of grape production. This study investigated the ecological impact of copper-containing fungicides on the accumulation and distribution of copper, manganese, chromium, and cobalt in the upper soil horizons of vineyards of varying ages in the Fruška Gora region, Serbia. The results indicated a marked difference in total copper content across vineyards, with the oldest vineyard exhibiting levels 6.9 times above the regulatory limit. Factor analysis delineated a strong correlation between copper accumulation and vineyard age while also highlighting the influence of landscape morphology on the spatial distribution of heavy metals. The findings suggest that copper accumulation is primarily related to agricultural practices, particularly the duration of fungicide application, while the distribution of other heavy metals is more closely associated with topographic features. The novelty of our research lies in the fact that we have shown that the assessment of copper accumulation in soil in vineyard ecosystems should take into account not only viticultural practices but also the history of land use and the landscape characteristics of the area.

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Sousa, Isadora Karolina Freitas de, Antonio Humberto Hamad Minervino, Rejane dos Santos Sousa, Dowglish Ferreira Chaves, Herbert Sousa Soares, Isabella de Oliveira Barros, Carolina Akiko Sato Cabral de Araújo, Raimundo Alves Barrêto Júnior et Enrico Lippi Ortolani. « Copper Deficiency in Sheep with High Liver Iron Accumulation ». Veterinary Medicine International 2012 (2012) : 1–4. http://dx.doi.org/10.1155/2012/207950.

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An outbreak of enzootic ataxia among sheep raised in the northeastern region of Brazil is described. Copper (Cu) deficiency was diagnosed in a herd of 56 sheep, among which five presented characteristic clinical symptoms of enzootic ataxia. The symptoms began 30 days after birth, with a clinical condition that included locomotion difficulty, limb ataxia, tremors, and continual falls. Liver biopsies were performed and blood was collected to determine hepatic and plasmatic Cu, iron (Fe), and zinc (Zn) concentration, respectively. The laboratory results showed that the animals presented low copper concentrations in the plasma and liver, without difference between the clinically healthy animals and those affected by enzootic ataxia. Even after supplementation with adequate Cu levels had been recommended, it was found on a new visit to the farm four months later that one animal still presented a clinical condition and that the hepatic Cu levels of the herd had not risen. Despite the low copper content of the diet, the high hepatic Fe levels found suggest that antagonism due to this element may have been an important factor in triggering copper deficiency in these animals, and thus, additional copper supplementation may be necessary for these animals.

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Espinoza, Daniela, Alberto González, Jaime Pizarro, Rodrigo Segura, Daniel Laporte, Fernanda Rodríguez-Rojas, Claudio A. Sáez et Alejandra Moenne. « Ulva compressa from Copper-Polluted Sites Exhibits Intracellular Copper Accumulation, Increased Expression of Metallothioneins and Copper-Containing Nanoparticles in Chloroplasts ». International Journal of Molecular Sciences 22, n^o 19 (29 septembre 2021) : 10531. http://dx.doi.org/10.3390/ijms221910531.

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In order to analyze the mechanisms involved in copper accumulation in Ulva compressa, algae were collected at control sites of central and northern Chile, and at two copper-polluted sites of northern Chile. The level of intracellular copper, reduced glutathione (GSH), phytochelatins (PCs), PC2 and PC4, and transcripts encoding metallothioneins (MTs) of U. compressa, UcMT1, UcMT2 and UcMT3, were determined. Algae of control sites contained around 20 μg of copper g−1 of dry tissue (DT) whereas algae of copper-polluted sites contained 260 and 272 μg of copper g−1 of DT. Algae of control sites and copper-polluted sites did not show detectable amounts of GSH, the level of PC2 did not change among sites whereas PC4 was increased in one of the copper-polluted sites. The level of transcripts of UcMT1 and UcMT2 were increased in algae of copper-polluted sites, but the level of UcMT3 did not change. Algae of a control site and a copper-polluted site were visualized by transmission electron microscopy (TEM) and the existence of copper in electrodense particles was analyzed using energy dispersive x-ray spectroscopy (EDXS). Algae of copper-polluted sites showed electrodense nanoparticles containing copper in the chloroplasts, whereas algae of control sites did not. Algae of a control site, Cachagua, were cultivated without copper (control) and with 10 μM copper for 5 days and they were analyzed by TEM-EDXS. Algae cultivated with copper showed copper-containing nanoparticles in the chloroplast whereas control algae did not. Thus, U. compressa from copper-polluted sites exhibits intracellular copper accumulation, an increase in the level of PC4 and expression of UcMTs, and the accumulation of copper-containing particles in chloroplasts.

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Ramos, Georgia Barros de A., et Yoko B. Rosato. « Copper accumulation in Xanthomonas campestris pv. vesicatoria ». Brazilian Journal of Genetics 19, n^o 4 (1996) : 551–54. http://dx.doi.org/10.1590/s0100-84551996000400002.

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White, C., et G. M. Gadd. « Copper accumulation by sulfate-reducing bacterial biofilms ». FEMS Microbiology Letters 183, n^o 2 (février 2000) : 313–18. http://dx.doi.org/10.1111/j.1574-6968.2000.tb08977.x.

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Pinho, G. L. L., C. M. G. Martins et I. Barber. « Copper accumulation by stickleback nests containing spiggin ». Environmental Science and Pollution Research 23, n^o 13 (10 mai 2016) : 13554–59. http://dx.doi.org/10.1007/s11356-016-6784-0.

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Kaitala, S. « Multiple Toxicity and Accumulation of Heavy Metals in Two Bivalve Mollusc Species ». Water Science and Technology 20, n^o 6-7 (1 juin 1988) : 23–32. http://dx.doi.org/10.2166/wst.1988.0185.

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Single and combined additions of copper (0.2 mg/l), zinc (0.4 mg/l) and manganese (2 mg/l) were used to examine the multiple accumulation and toxicity of these metals in sessile blue mussel Mytilus edulis L. and burrowing clam Macoma baltica (L.) in a 23-factorial experiment. Concentrations of metals were analysed with atom absorption spectrometry. The accumulation of metals and their toxicity were determined by regression analyses. The presence of copper ions stimulated the accumulation of zinc and manganese in mussels so that the concentration of manganese was 100 % and zinc 25 % higher than without copper additions. In accumulation of manganese and copper in clams, other metals did not have any effect and zinc did not accumulate at all. The values of lethality (LC50) were 127/µg Cu/l for mussels in 8 days and 54 µg Cu/l for clams in 10 days.

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Троц, Наталья, N. Trots, Оксана Горшкова et Oksana Gorshkova. « THE ACCUMULATION OF HEAVY METALS BY SPRING WHEAT SEEDLINGS ». Bulletin Samara State Agricultural Academy 2, n^o 3 (27 juillet 2017) : 7–11. http://dx.doi.org/10.12737/17444.

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The purpose of this study is to develop agrotechnological methods of establishing and controlling the content of heavy metals in plant products. For laboratory experiments we used the typical black soil. The results of lead and copper in soil and plants of spring wheat variety Kinelskaya 59, with different levels of pollution in the conditions of a pot experiment. It is established that with the increase of applied doses of salts of heavy metals increases their content in soil and spring wheat seedlings. The percentage of mobility in the soil of copper and lead in the soil increases with the pollution level 2.9 times and 9.2 times, respectively. With increasing doses of copper and lead increases the concentration of plant-available forms of nitrogen and phosphorus. For all variants of experience is typical acropetal distribution of heavy metals in plants – copper: the roots (46.59) > shoots (of 14.66); lead: the roots (47.0) > shoots (12.78). The value of Iа decreases with increasing dose of applied salt that is indicative of the culture of spring wheat variety Kinelskaya 59 barrier. High correlation observed between the content of copper in plant roots and gross values (r = 0.98) and mobile forms(r = 0.94) in the soil. The value of copper in the shoots of plants are weaker related to the content of the element in the soil (r = 0.66). Between the content of gross and mobile forms of lead in soil revealed a high correlation (r = 1.00), the same value when item is marked with the plant roots and shoots.

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Yun, Sumin, Dohee Lee, Jimin Oh, Yeon Chae, Taesik Yun, Yoonhoi Koo, Mhan-Pyo Yang, Byeong-Teck Kang et Hakhyun Kim. « Primary copper-associated chronic hepatitis without copper metabolism domain containing 1 mutation in a Dalmatian : a case report ». Korean Journal of Veterinary Research 62, n^o 4 (31 décembre 2022) : e31. http://dx.doi.org/10.14405/kjvr.20220020.

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A 12-year-old intact male Dalmatian dog presented hyporexia and vomiting for 1 week. Blood analysis revealed increased liver enzyme activity. Histopathological examination of the liver confirmed chronic hepatitis with fibrosis and necrosis. Copper staining revealed marked copper accumulation (2,770 ppm; normal range, 200 to 400 ppm), prominent in the centrilobular region, and compatible with copper-associated chronic hepatitis. However, copper metabolism domain containing 1 (COMMD1) mutation predisposing to copper accumulation in the liver tissue was not identified. The dog received medications but died 1 month after first visit. This is the first case of primary copper-associated hepatitis without COMMD1 mutation in a Dalmatian dog in South Korea.

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Bagatto, G., et J. D. Shorthouse. « Accumulation of copper and nickel in plant tissues and an insect gall of lowbush blueberry, Vaccinium angustifolium, near an ore smelter at Sudbury, Ontario, Canada ». Canadian Journal of Botany 69, n^o 7 (1 juillet 1991) : 1483–90. http://dx.doi.org/10.1139/b91-192.

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The accumulation of copper and nickel in plant tissues and galls of Hemadas nubilipennis on lowbush blueberry near an ore smelter at Sudbury, Ontario, was investigated. Concentrations of these metals in the root, stem, and leaf tissue decline logarithmically with increasing distance from the Sudbury smelter. The pattern of accumulation for copper and nickel in the various tissues was root > stem > leaf > berry; however, metal differences in these tissues were not as great in plants farther from the smelter. The root tissue is the primary site of accumulation of these metals when environmental levels of copper and nickel are high. The highest concentrations of copper and nickel were found in the galls, indicating that gall tissues act as a strong physiological sink for micronutrients and redirect nutrients from the host plant. Key words: Vaccinium angustifolium, copper, nickel, gall, Sudbury.

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Waldrop, G. L., et M. J. Ettinger. « The relationship of excess copper accumulation by fibroblasts from the brindled mouse model of Menkes disease to the primary defect ». Biochemical Journal 267, n^o 2 (15 avril 1990) : 417–22. http://dx.doi.org/10.1042/bj2670417.

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Fibroblasts from the brindled mouse model of Menkes disease are known to accumulate excess copper. Most of the copper in the cytosol of these fibroblasts is bound to metallothionein (MT), which is elevated in Menkes or brindled mouse fibroblasts. Copper accumulation by normal fibroblasts containing excess MT was examined to determine if the excess copper accumulation phenotype was secondary to excess MT or associated with the primary defect in fibroblasts from the brindled mice. MT was induced in normal fibroblasts by copper, zinc or dexamethasone to levels comparable with those in brindled mice fibroblasts, as determined by radioimmunoassays. Normal fibroblasts containing excess MT accumulate copper normally, i.e. they do not exhibit the excess copper accumulation phenotype. Consistent with this result, copper efflux from normal fibroblasts containing excess MT was also normal. The data suggest that one function of the protein associated with the primary defect is to help determine how much copper is taken up and retained by fibroblasts and other cell types exhibiting the excess copper phenotype in Menkes disease. The capacity of this protein is apparently exceeded in normal fibroblasts if serum or albumin is not present extracellularly to limit total copper uptake. Consistent with a defect in an intracellular protein, the kinetics of copper transport by brindled mice fibroblasts were found to be normal.

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Moenne, Alejandra, Melissa Gómez, Daniel Laporte, Daniela Espinoza, Claudio A. Sáez et Alberto González. « Mechanisms of Copper Tolerance, Accumulation, and Detoxification in the Marine Macroalga Ulva compressa (Chlorophyta) : 20 Years of Research ». Plants 9, n^o 6 (27 mai 2020) : 681. http://dx.doi.org/10.3390/plants9060681.

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Copper induces an oxidative stress condition in the marine alga Ulva compressa that is due to the production of superoxide anions and hydrogen peroxide, mainly in organelles. The increase in hydrogen peroxide is accompanied by increases in intracellular calcium and nitric oxide, and there is a crosstalk among these signals. The increase in intracellular calcium activates signaling pathways involving Calmodulin-dependent Protein Kinases (CaMKs) and Calcium-Dependent Protein Kinases (CDPKs), leading to activation of gene expression of antioxidant enzymes and enzymes involved in ascorbate (ASC) and glutathione (GSH) synthesis. It was recently shown that copper also activates Mitogen-Activated Protein Kinases (MAPKs) that participate in the increase in the expression of antioxidant enzymes. The increase in gene expression leads to enhanced activities of antioxidant enzymes and to enhanced levels of ASC and GSH. In addition, copper induces an increase in photosynthesis leading to an increase in the leve of Nicotinamide Adenine Dinucleotide Phosphate (NADPH). Copper also induces an increase in activities of enzymes involved in C, N, and S assimilation, allowing the replacement of proteins damaged by oxidative stress. The accumulation of copper in acute exposure involved increases in GSH, phytochelatins (PCs), and metallothioneins (MTs) whereas the accumulation of copper in chronic exposure involved only MTs. Acute and chronic copper exposure induced the accumulation of copper-containing particles in chloroplasts. On the other hand, copper is extruded from the alga with an equimolar amount of GSH. Thus, the increases in activities of antioxidant enzymes, in ASC, GSH, and NADPH levels, and in C, N, and S assimilation, the accumulation of copper-containing particles in chloroplasts, and the extrusion of copper ions from the alga constitute essential mechanisms that participate in the buffering of copper-induced oxidative stress in U. compressa.

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Borgmann, U., et W. P. Norwood. « Toxicity and accumulation of zinc and copper in Hyalella azteca exposed to metal-spiked sediments ». Canadian Journal of Fisheries and Aquatic Sciences 54, n^o 5 (1 mai 1997) : 1046–54. http://dx.doi.org/10.1139/f97-020.

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The relationship between zinc and copper toxicity in Hyalella azteca and accumulation from metal-spiked sediments was determined for future use in identifying sites where these metals contribute to sediment toxicity. Both zinc and copper accumulation increased rapidly with increasing sediment concentrations, showing no evidence of saturation as seen in waterborne exposures. Zinc accumulation from zinc spiked sediments was substantial, even at concentrations below those causing chronic toxicity, and body concentrations can readily be used to infer chronic toxicity. Chronic toxicity in spiked sediment exposures occurred at excess (above background) body concentrations twice as high as in waterborne exposures, possibly as a result of zinc in gut contents. In contrast, chronic copper toxicity occurred at body concentrations lower than those observed in waterborne exposures. Furthermore, body copper at low sediment exposures was independent of copper in sediment. Body copper concentrations can be used to infer short-term (1-week) toxicity but not chronic toxicity. Copper, but not zinc, caused a reduction in growth, even at concentrations below the 4-week LC25. Reduced growth can, therefore, be used to differentiate between chronic copper and zinc toxicity in sediments.

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IRAWATI, WAHYU, TRIWIBOWO YUWONO et NOMMENSEN PANGIHUTAN OMPUSUNGGU. « Growth characteristics and copper accumulation of bacterial consortium Acinetobacter sp. and Cupriavidus sp. isolated from a wastewater treatment plant ». Biodiversitas Journal of Biological Diversity 19, n^o 5 (21 septembre 2018) : 1884–90. http://dx.doi.org/10.13057/biodiv/d190541.

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Irawati W, Yuwono T, Ompusunggu NP. 2018. Growth characteristics and copper accumulation of bacterial consortium Acinetobacter sp. and Cupriavidus sp. isolated from a wastewater treatment plant. Biodiversitas 19: 1884-1890. Pollutant treatments are part of the human calling, as the crown of creation, to subdue, preserve, and cultivate the earth in bringing goodness for all creatures. Bioremediation of copper using indigenous bacteria is well known as the best water treatment for polluted environment recovery. Acinetobacter sp. and Cupriavidus sp. are indigenous bacteria isolated from industrial sewage in Indonesia. Bioremediation in environment is a process involving community of bacterial consortium for heavy metal or any other polluting materials accumulation. The purposes of this research were: (i) to characterize growth of Acinetobacter sp. and Cupriavidus sp. consortia in sewage medium, enrichment medium, and medium supplemented with copper, (ii) to establish the potency of bacterial consortia to accumulate copper. The growth of bacteria was observed based on cell turbidity using spectrophotometer at wavelength of 600 nm. Cells pellet was destructed by nitric acid at 100oC and copper concentration was analyzed by atomic absorption spectrophotometer as copper accumulation value. The results showed that the growth of bacterial consortia in medium containing copper was better than that of single bacterium. The best bacterial consortium was the mixture of Acinetobacter sp. IrC1 and Acinetobacter sp. IrC2. The use of sewage as cultivation medium decreased bacterial growth by up to 25% but still resulted in the same level of logarithmic phase in enrichment medium. The highest accumulation capability was of a consortium of Acinetobacter sp. IrC1 and Acinetobacter sp. IrC2 at a level of 6.45 g/mg copper/g cells dry weight, suggesting that 5.09% of copper were accumulated by cells. It was concluded that the best composition of consortia in growth and copper accumulation capability was the mixture of Acinetobacter sp. IrC1 and Acinetobacter sp. IrC2. These results may be due to the fact that both bacteria belong to the same genus that allowed them for synergistic interactions.

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Tsekova, Kolishka, et Dessislava Todorova. « Copper (II) Accumulation And Superoxide Dismutase Activity During Growth Of Aspergillus Niger B-77 ». Zeitschrift für Naturforschung C 57, n^o 3-4 (1 avril 2002) : 319–22. http://dx.doi.org/10.1515/znc-2002-3-421.

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The influence of copper (II) ions on the growth, accumulation properties and superoxide dismutase (SOD) activity of a growing culture of Aspergillus niger B-77 were studied. Microbial growth, the level of copper (II) accumulation and SOD activity depended on the initial copper (II) concentration. Aspergillus niger is able to accumulate large amounts of copper (II) from the nutrient medium with 200 mg.l-1 copper (II) ions without loosing its biological activities. Addition of copper (II) ions increased the SOD activity in the growing cell cultures. The changes in enzyme activity induced by heavy metal ions might be used as an indicator of intracellular oxy-intermediate generation in a cell culture growing under stress conditions

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Melendez, Meredith V., Joseph R. Heckman, Stephanie Murphy et Frank D’Amico. « New Jersey Farm Soil Copper Levels Resulting from Copper Fungicide Applications ». HortTechnology 30, n^o 2 (avril 2020) : 268–72. http://dx.doi.org/10.21273/horttech04494-19.

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Vegetable growers have expressed concerns regarding the accumulation of copper in soil where copper-based fungicides are used and have requested guidance for copper pesticide applications. Elevated soil copper levels have the potential to become toxic to sensitive crops and impact soil health. In response, total and available soil copper levels were surveyed using soil analysis of samples from 15 New Jersey farms representing organic and conventional production methods. Lettuce (Lactuca sativa) was grown in the sampled soil in a greenhouse trial and evaluated for signs of copper toxicity. We found that all 15 farms were using copper fungicide preventative sprays during the previous 2 years. The soil copper levels of these farms were higher in copper-applied soils than the corresponding noncopper-applied soil. Soil copper levels were not near or in excess of established clean-up limits at any of the locations. Greenhouse-grown lettuce in the sampled soils was not negatively impacted by the copper levels. Due to the increase in the total and soluble soil copper levels, growers should use best management practices to prevent the accumulation of excessive amounts of copper in the soil over time.

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Durumin Iya, N. I., Z. B. Assim, O. A. Omorinoye et E. A. Asare. « Phytoextraction of copper and lead from spiked soil using Acalypha wilkesiana (Copper leaf) and Polyscias fruticosa (Aralia) ». Dutse Journal of Pure and Applied Sciences 8, n^o 2a (25 juin 2022) : 187–96. http://dx.doi.org/10.4314/dujopas.v8i2a.18.

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Soil contaminated with heavy metals industrially or anthropogenically is a major global concern. This study aimed at investigating the potential of Acalypha wilkesiana and Polyscias fruticosa plants to remove copper (Cu) and lead (Pb) from soil spiked with solutions of Cu and Pb. Information on the ability of both plants to uptake and accumulate Cu and Pb from spiked soil is insufficient. Plant cuttings were grown in a 2 kg soil spiked with heavy metals and kept at the greenhouse for the period of 240 days. Root, stem and leaf of the plants were analyzed for Cu and Pb using Atomic Absorption Spectrophotometer (AAS). Cu accumulation (2.58 -542.66 mg/kg) was appreciably higher than Pb accumulation (26.24 - 46.44 mg/kg) in A. wilkesiana, while Cu accumulation ranged 4.54 - 384.19 mg/kg was found to be lower than the Pb accumulation ranged 1.22-759.48 mg/kg in P. fruticosa. The maximum accumulation of Cu and Pb in the shoot were found to be 52.50 and 82.93 mg/kg in A. wilkesiana, while 38.26 and 123.98 mg/kg in P. fruticosa. Accumulation of Cu and Pb by both plants were correlated using the indices bioaccumulation, transfer factor and translocation index. The result is encouraging and suggests that both plants could be applied for phytoextraction of Cu and Pb from heavy metal spiked soil.

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Sebezhko, Ol'ga, Tatyana Konovalova, Olga Korotkevich, Valeriy Petuhov, Alena Sebezhko et Ol'ga Zayko. « GENETIC DETERMINATION OF COPPER ACCUMULATION IN MYOCARDIUM IN CATTLE OF WESTERN SIBERIA ». Bulletin of KSAU, n^o 10 (6 février 2025) : 160–66. https://doi.org/10.36718/1819-4036-2023-10-160-166.

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The purpose of the study is to assess the hereditary nature of copper accumulation in the heart muscle of Holstein bulls bred in the ecological and climatic conditions of Western Siberia. The results of assessing the level of copper in the myocardium of Holstein bulls aged 12–13 months, which are the descendants of 4 sires, are presented. Blood was taken from animals with a live weight of 330–365 kg, bred in a large livestock enterprise located in Western Siberia. The level of copper in the myocardium of bulls was determined by atomic absorption spectrometry with flame and electrothermal atomization. One-way analysis of variance was used to determine differences between groups. η2 was used as a test to assess the effect size, and Tukey’s test was used for post hoc comparisons. It was found that the average copper content in the myocardium of Holstein bulls was 3.24 ± 0.04 mg/kg. Phenotypic variability of copper concentration in the heart muscle was characterized by low values. The established differences (p-value < 0.05) in copper content in the offspring of different sires reflect the genetic determination of the accumulation of this metal in the heart muscle. The strength of influence of the factor of paternal affiliation of bulls on the level of copper content in the myocardium was 35 %. The level of copper in the myocardium was higher by 0.52 mg/kg in the offspring of the bull Fabio compared to the sons of Brio. The reference interval for myocardial copper content based on the central 95 % percentile with 90 % confidence intervals was 2.9 (2.78 – 3.02) – 3.7 (3.57–3.82) mg/kg.

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van Ryssen, J. B. J., et P. R. Barrowman. « Effect of ionophores on the accumulation of copper in the livers of sheep ». Animal Science 44, n^o 2 (avril 1987) : 255–61. http://dx.doi.org/10.1017/s0003356100018626.

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ABSTRACTLambs were allocated to three treatments: control (C), monensin (M) and lasalocid sodium (L) in a concentrate diet containing 36 mg copper per kg food. After 2 months deaths from copper toxicity occurred and the trial was terminated. All lambs were slaughtered. The mean liver copper concentration (mg/kg dry matter) of 1907 in the M treatment was higher than the mean concentrations of 1342 and 1229 in the C and L groups respectively (P < 0·01). Total liver copper was 234, 260 and 230 mg and the retention of dietary copper in the liver was 93·5, 108·5 and 93·3 g/kg for the C, M and L treatments respectively. These differences were not significant. From reduced liver size (as a proportion of body weight) and high copper levels in the kidneys, it was concluded that the group given monensin had accumulated copper to a level closer to the toxicity threshold than the other two groups.

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Wairich, Andriele, Lessandro De Conti, Thainá I. Lamb, Rosana Keil, Leonardo O. Neves, Gustavo Brunetto, Raul A. Sperotto et Felipe K. Ricachenevsky. « Throwing Copper Around : How Plants Control Uptake, Distribution, and Accumulation of Copper ». Agronomy 12, n^o 5 (21 avril 2022) : 994. http://dx.doi.org/10.3390/agronomy12050994.

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Copper (Cu) is essential to plants but can also be harmful due to Fenton chemistry. Because of that, it is necessary to keep Cu within a narrow concentration limit. Plants evolved mechanisms to sense Cu and precisely regulate uptake and accumulation to avoid both deficiency and toxicity. However, plants experience variable Cu levels in soils, both due to natural environments as well as human’s-based farming practices that can lead to Cu accumulation in soils. Therefore, we need to understand Cu homeostasis. Here, we review how Cu is found in soils; Cu toxicity responses in plants; the role of Cu transporters, described mainly in model species such as Arabidopsis thaliana and Oryza sativa; the crosstalk between Cu and iron (Fe) homeostasis; Cu hyperaccumulator plants; and discuss some gaps and future directions, which can improve our understanding in the field.

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Ito, Hiroyasu, Masahiro Inouhe, Hiroshi Tohoyama et Masanori Joho. « Copper Extrusion after Accumulation during Growth of Copper-Tolerant Yeast Yarrowia lipolytica ». Zeitschrift für Naturforschung C 62, n^o 1-2 (1 février 2007) : 77–82. http://dx.doi.org/10.1515/znc-2007-1-214.

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The Cu2+-tolerant yeast Yarrowia lipolytica accumulated Cu2+ until the late logarithmic phase. Thereafter, Cu2+ was temperature-dependently extruded into phosphate-limited culture medium containing high concentrations of heavy metal ions but not into 10 mm 2-(Nmorpholino) ethane sulfonic acid (MES) buffer (pH 6.0). Peptone in the culture medium played an important role in the extrusion, which proceeded even when peptone was substituted with cysteine or histidine, but not with any other amino acid tested.

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Masaldan, Shashank, Sharnel A. S. Clatworthy, Cristina Gamell, Zoe M. Smith, Paul S. Francis, Delphine Denoyer, Peter M. Meggyesy, Sharon La Fontaine et Michael A. Cater. « Copper accumulation in senescent cells : Interplay between copper transporters and impaired autophagy ». Redox Biology 16 (juin 2018) : 322–31. http://dx.doi.org/10.1016/j.redox.2018.03.007.

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Beynen, A. C., S. Yu et R. Van Der Meer. « Excessive Hepatic Copper Accumulation in Jaundiced Rats Fed a High-Copper Diet ». Biological Trace Element Research 88, n^o 3 (2002) : 255–70. http://dx.doi.org/10.1385/bter:88:3:255.

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Martins, Samantha Eslava Gonçalves, et Adalto Bianchini. « Copper accumulation and toxicity in the yellow clam Mesodesma mactroides DESAHYES 1854 (BIVALVIA, MESODESMATIDAE) ». Natural Resources 3, n^o 2 (8 janvier 2014) : 15. http://dx.doi.org/10.6008/ess2237-9290.2013.002.0010.

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The yellow clam Mesodesma mactroides depends on the intertidal zone of the southernmost Brazilian sandy beaches for recruitment or settlement. Studies aiming to evaluate any kind of alteration of this ecosystem are very important because of the growing industrial and urban development observed around this region over the last decades. Therefore, the main goal of the present study was to evaluate copper accumulation and toxicity in M. mactroides to verify its response to increased environmental levels of this metal. Yellow clams were acutely (96 h) exposed to copper for determination of copper accumulation and toxicity (LC50). Results showed that M. mactroides accumulated copper in its tissues (gills and digestive gland) and was quite tolerant to copper, suggesting this species as a suitable model for future biomonitoring programs of copper contamination in sandy beaches.

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DONICI, Alina, Claudiu Ioan BUNEA, Anamaria CĂLUGĂR, Eugenia HARSAN et Florin Dumitru BORA. « Investigation of the Copper Content in Vineyard Soil, Grape, Must and Wine in the Main Vineyards of Romania : a Preliminary Study ». Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca. Horticulture 76, n^o 1 (12 juin 2019) : 31. http://dx.doi.org/10.15835/buasvmcn-hort:2018.0017.

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The long-term use of copper in viticulture has caused great copper accumulation in vineyard soils, resulting in negative effects on the environment through toxicity to aquatic and soil organisms. The aim of this study was to investigate the copper content in vineyard soils, grapes and wines from Dealu Bujorului, Murfatlar, Tarnave, Iasi and Ştefăneşti vineyards. The ICP-MS method was used for copper determination in vineyard soil, grape must and wine. Copper concentration in red wine samples was significantly higher than in the white wine samples. Values for Transfer Factor and Mobility Ratio indicates that Vitis vinifera L. does not allow the accumulation of copper from vineyard soil in must grape and wine. The copper concentration in grapes, must and wine has been influenced by the copper concentration in soils, by copper fungicide used to protect the vine and by other factors such as the biological specificity of cultivars during growth.

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Chiou et Hsu. « Copper Toxicity and Prediction Models of Copper Content in Leafy Vegetables ». Sustainability 11, n^o 22 (6 novembre 2019) : 6215. http://dx.doi.org/10.3390/su11226215.

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Copper (Cu), a toxic metal pollution found in the soil and water of industrialized areas, causes continuous issues for agriculture product contamination and human health hazards. However, information on copper phytotoxicity and its accumulation in vegetables is largely unknown. To evaluate the related agricultural loss and health risks, it is necessary to assess copper phytotoxicity and develop prediction models for copper concentration in vegetables. Here, we assess the growth performance and copper concentration of four leafy vegetables: Water spinach, amaranth, pakchoi, and garland chrysanthemum in copper-contaminated soil. The plant’s height and fresh weight is dramatically reduced when the soil copper concentration is over ~250 mg·kg−1. This yield reduction and copper accumulation are associated with an increase of soil copper concentration, suggesting high copper phytotoxicity levels in plants and soil. The prediction models of plant copper concentration were developed using multiple regressions based on one-step extractions of the soil copper as independent variables. One prediction model derived for amaranth copper using hydrochloric acid (HCl)-extractable and ethylenediaminetetraacetic acid (EDTA)-extractable copper from soil is able to describe 78.89% of the variance in the measured copper. As a result, the phytotoxic copper level for four leafy vegetables is revealed. Although the prediction models may not be universal, the predicted and phytotoxic copper levels are useful tools for evaluating vegetable yield and daily copper intake.

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Liu, Weiping, Yikun Yang, Huizhou Xiong, Ying Lu et Rong Yang. « Distribution of Copper in Rats Submitted to Treatment With Copper Aspirinate ». Metal-Based Drugs 5, n^o 6 (1 janvier 1998) : 333–35. http://dx.doi.org/10.1155/mbd.1998.333.

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The distribution of copper in Sprague – Dawley rats following a three month oral administration of 0,10 or 50mg/kg copper aspirinate has been investigated. Metal content was determined by ICP – AES in blood, brain, kidney, liver, lung, spleen, and dejection. The results show that treatment with copper aspirinate did not cause accumulation of copper in rats and excess ingested copper was excreted through feces.

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León Morales, Janet M., Mario Rodríguez-Monroy et Gabriela Sepúlveda-Jiménez. « Betacyanin accumulation and guaiacol peroxidase activity in Beta vulgaris L. leaves following copper stress ». Acta Societatis Botanicorum Poloniae 81, n^o 3 (2012) : 193–201. http://dx.doi.org/10.5586/asbp.2012.019.

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The effect of copper stress on betacyanin accumulation and guaiacol peroxidase (GPOD) activity in leaves of different age was evaluated in red beet (<em>Beta vulgaris </em>L. var. Crosby Egyptian) plants. In hydroponic culture, plants were treated with 0.3 μM (control), 50 μM, 100 μM, and 250 μM of CuSO<sub>4</sub> for 6 days. Copper was taken up and accumulated in old roots but was not translocated to leaves. However in young leaves, the increase of lipid peroxidation and reduction of growth were evident from day 3 of copper exposure; whereas in old leaves, the lipid peroxidation and growth were the same from either copper-treated or control plants. In response to copper exposure, the betacyanin accumulation was evident in young leaves by day 3, and continued to increase until day 6. Betacyanin only were accumulated in old leaves until day 6, but the contents were from 4 to 5 times lower than those observed in young leaves at the same copper concentrations. GPOD activity increased 3.3- and 1.4-fold in young and old leaves from day 3 of copper treatment respectively, but only in the young leaves was sustained at the same level until day 6. Old roots shown betacyanin in the control plants, but the betacyanin level and growth were reduced with the copper exposure. In contrast, young roots emerged by copper effect also accumulated copper and showed the highest betacyanin content of all plant parts assayed. These results indicate that betacyanin accumulation and GPOD activity are defense responses to copper stress in actively growing organs.

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Bagwell, C. E., C. E. Milliken, S. Ghoshroy et D. A. Blom. « Intracellular Copper Accumulation Enhances the Growth of Kineococcus radiotolerans during Chronic Irradiation ». Applied and Environmental Microbiology 74, n^o 5 (11 janvier 2008) : 1376–84. http://dx.doi.org/10.1128/aem.02175-07.

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ABSTRACT The actinobacterium Kineococcus radiotolerans is highly resistant to ionizing radiation, desiccation, and oxidative stress, though the underlying biochemical mechanisms are unknown. The purpose of this study was to explore a possible linkage between the uptake of transition metals and extreme resistance to ionizing radiation and oxidative stress. The effects of six different divalent cationic metals on growth were examined in the absence of ionizing radiation. None of the metals tested were stimulatory, though cobalt was inhibitory to growth. In contrast, copper supplementation dramatically increased colony formation during chronic irradiation. K. radiotolerans exhibited specific uptake and intracellular accumulation of copper, compared to only a weak response to both iron and manganese supplementation. Copper accumulation sensitized cells to hydrogen peroxide. Acute-irradiation-induced DNA damage levels were similar in the copper-loaded culture and the age-synchronized no-copper control culture, though low-molecular-weight DNA was more persistent during postirradiation recovery in the Cu-loaded culture. Still, the estimated times for genome restoration differed by only 2 h between treatments. While we cannot discount the possibility that copper fulfills an unexpectedly important biochemical role in a low-radioactivity environment, K. radiotolerans has a high capacity for intracellular copper sequestration and presumably efficiently coordinated oxidative stress defenses and detoxification systems, which confers cross-protection from the damaging effects of ionizing radiation.

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