Bibliographies thématiques / CRISPR/Cas9, flow cytometry, order of event

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Littérature scientifique sur le sujet « CRISPR/Cas9, flow cytometry, order of event »

Auteur : Grafiati

Publié le 14 mars 2023

Mis à jour le 31 juillet 2025

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Articles de revues sur le sujet "CRISPR/Cas9, flow cytometry, order of event"

Adriaanse, Fabienne R. S., Sadie M. Sakurada, Shondra M. Pruett-Miller, Ronald W. Stam, Michel C. Zwaan, and Tanja A. Gruber. "Non-Coding HOX Fusions in Pediatric Non-Down Syndrome Acute Megakaryoblastic Leukemia." Blood 134, Supplement_1 (2019): 533. http://dx.doi.org/10.1182/blood-2019-127014.

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The homeobox (HOX) genes are a highly conserved family of transcription factors involved in embryonic patterning as well as adult hematopoiesis. Dysregulation of HOX genes, in particular upregulation of HOXA cluster genes, is a frequent event in Acute Myelogenous Leukemia (AML). Recently, we performed a detailed genomic analysis on pediatric non-Down Syndrome Acute Megakaryoblastic Leukemia (non-DS-AMKL) and identified novel fusions involving a HOX cluster gene in 14.9% of the cases. While most fusions were predicted to lead to an in-frame functional protein, several fusions included a non-cod

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Ivy, Kathryn S., Candace H. Cote, and Paul Brent Ferrell. "IDH2 Mutations Induce Altered STAT Signaling and Cytokine Responses Which Are Restored By Enasidenib." Blood 132, Supplement 1 (2018): 1468. http://dx.doi.org/10.1182/blood-2018-99-117783.

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Abstract Acute myeloid leukemia (AML) is a heterogeneous myeloid malignancy characterized by mutational and clonal heterogeneity. Mutations in isocitrate dehydrogenase 1 and 2 (IDH1/2) are common, occurring in approximately 15-20% of patients, and actionable, with recently approved inhibitors for both mutations. These inhibitors lead to leukemia cell differentiation in vitro, in vivo and in patients. Healthy myeloid differentiation is governed by precise regulation of intracellular signaling, but this regulation is disrupted in AML. Given that signal transducer and activator of transcription (

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Pabst, Gabriel, Johannes Foßelteder, Angelika Schlacher, et al. "Modeling the Development of SRSF2 Mutated Myeloid Malignancies By CRISPR/Cas9 Mediated Genome Engineering of Primary Human Hematopoietic Stem and Progenitor Cells." Blood 138, Supplement 1 (2021): 2160. http://dx.doi.org/10.1182/blood-2021-149591.

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Abstract Introduction: Acute Myeloid Leukemia (AML) is a malignant disease of the bone marrow that can arise from a premalignant condition called clonal hematopoiesis of indeterminate potential (CHIP). Mutations in Serine and Arginine-rich Splicing Factor 2 (SRSF2) are detected in CHIP and mediate a high risk for AML development. Here we used CRISPR/Cas9-mediated genome engineering to introduce a heterozygous SRSF2P95H mutation into primary human hematopoietic stem and progenitor cells (HSPCs) and investigated its functional consequences using both in vitro and in vivo assays. Methods: We used

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Xiong, Kun. "Feasibility assessment of using cRISPR-Cas9 to improve the infiltration of CAR-T cells in solid tumors." Theoretical and Natural Science 60, no. 1 (2024): 46–51. http://dx.doi.org/10.54254/2753-8818/60/20241395.

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Abstract. Chimeric antigen receptor T-cell immunotherapy (CAR-T) has been developing for decades, CAR-T is playing an increasingly important role in tumor treatment. However, because fibroblasts (CAFs) in solid tumors secrete proteins and glycans to form ECM, CAR-T is faced with the challenge of improving tumor invasion. To this end, a new scheme was put forth to alter CAR T cells such that they release heparinase (HPSE) to break down heparan sulfate proteoglycan (HSPG), which is covered on the outermost layer of the cancerous cells by CAF and released. In order to solve the above problems, CR

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Lanjewar, S. N., and K. R. Bondioli. "205 Optimization of Transfection Efficiency for CRISPR/Cas9-Induced Genomic Editing in Porcine Fibroblast Cells." Reproduction, Fertility and Development 30, no. 1 (2018): 243. http://dx.doi.org/10.1071/rdv30n1ab205.

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The clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated (Cas9) system creates DNA double-stranded breaks (DSB) at specific sequences and allows efficient genomic modification, even in species previously resistant to gene editing. The DSB can be repaired using non-homologous end joining (creating insertions/deletions) or by homology directed repair (HDR) using a donor DNA with small changes at the cut site, giving rise to precise targeted modifications. Despite growing interest in genome editing using RNA-guided endonucleases, the efficiency of HDR is only 0.5 t

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Haase-Kohn, Cathleen, Markus Laube, Cornelius K. Donat, Birgit Belter, and Jens Pietzsch. "CRISPR/Cas9 Mediated Knockout of Cyclooxygenase-2 Gene Inhibits Invasiveness in A2058 Melanoma Cells." Cells 11, no. 4 (2022): 749. http://dx.doi.org/10.3390/cells11040749.

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The inducible isoenzyme cyclooxygenase-2 (COX-2) is an important hub in cellular signaling, which contributes to tumor progression by modulating and enhancing a pro-inflammatory tumor microenvironment, tumor growth, apoptosis resistance, angiogenesis and metastasis. In order to understand the role of COX-2 expression in melanoma, we investigated the functional knockout effect of COX-2 in A2058 human melanoma cells. COX-2 knockout was validated by Western blot and flow cytometry analysis. When comparing COX-2 knockout cells to controls, we observed significantly reduced invasion, colony and sph

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Beigl, Tobias B., Ine Kjosås, Emilie Seljeseth, Nina Glomnes, and Henriette Aksnes. "Efficient and crucial quality control of HAP1 cell ploidy status." Biology Open 9, no. 11 (2020): bio057174. http://dx.doi.org/10.1242/bio.057174.

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ABSTRACTThe near-haploid human cell line HAP1 recently became a popular subject for CRISPR/Cas9 editing, since only one allele requires modification. Through the gene-editing service at Horizon Discovery, there are at present more than 7500 edited cell lines available and the number continuously increases. The haploid nature of HAP1 is unstable as cultures become diploid with time. Here, we demonstrated some fundamental differences between haploid and diploid HAP1 cells, hence underlining the need for taking control over ploidy status in HAP1 cultures prior to phenotyping. Consequently, we opt

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Foßelteder, Johannes, Angelika Schlacher, Gabriel Pabst, et al. "Introduction and Genetic Correction of Calreticulin Mutations in Human Hematopoietic Stem and Progenitor Cells Sheds Light on MPN Pathogenesis." Blood 138, Supplement 1 (2021): 2541. http://dx.doi.org/10.1182/blood-2021-147919.

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Abstract Introduction: Recurrent mutations in calreticulin (CALR) are present in 70% to 80% of essential thrombocythemia (ET) and primary myelofibrosis (PMF) patients without a JAK2 or MPL mutation. Despite recent advances in understanding mutant CALR, the detailed mechanisms are not fully elucidated, and current knowledge is mainly based on transgenic mouse models or human cancer cell lines. Thus, to more faithfully model MPN pathogenesis, we first aimed to introduce heterozygous type-1 and type-2 CALR mutations into healthy human hematopoietic stem and progenitor cells (HSPCs) via targeted C

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Castro, Jesus, Mariana Gaelzer, and Scott Welford. "Abstract 209: Elucidating the role of NMDA subunit NR2B in non-tumor and tumor-bearing mice." Cancer Research 82, no. 12_Supplement (2022): 209. http://dx.doi.org/10.1158/1538-7445.am2022-209.

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Abstract Therapeutic options for glioblastoma multiforme (GBM), the most common and fatal brain tumor, have been limited over the course of recent decades, leaving radiation as one of the few effective therapies. While advancements in radiation therapy have improved life expectancy and patient outcome, exposure to radiotherapy causes normal tissue toxicity and damage to the central nervous system, resulting in cognitive impairment and negative patient side effects. In the hippocampus, the memory processing center of the brain, radiotherapy-induced elevated glutamate levels lead to over-excitat

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Soerensen, Johannes Frasez, Carina Agerbo Rosenberg, Katharina Wolter, et al. "Assessing Lipid Nanoparticle RNA Delivery Including CRISPR-Cas9 Based Therapy to Bone Marrow Cells with Emphasis on Leukemic Blasts - a Proof-of-Principle Study." Blood 144, Supplement 1 (2024): 7451. https://doi.org/10.1182/blood-2024-199992.

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Acute myeloid leukemia (AML) with the translocation t(8;21)(q22;q22.1) resulting in the fusion oncogene RUNX1::RUNX1T1 is a well-described subtype of AML. Generally perceived as associated with a favorable prognosis, the main cause of mortality in these patients remain relapse, occurring in an estimated 40% of patients leading to increased mortality. Gene editing technology CRISPR-Cas9, has in previous research been demonstrated to be able to disrupt the RUNX1::RUNX1T1 fusion gene. The disruption leads to inhibited leukemic cell growth and proliferation, suggesting its potential as a future th

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Thèses sur le sujet "CRISPR/Cas9, flow cytometry, order of event"

Romitelli, Antonia. "A CRISPR-based flow cytometric approach to to assess the order of transcriptional events." Doctoral thesis, Università di Siena, 2022. http://hdl.handle.net/11365/1195095.

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Recently many devices have been developed to record and store information in living cells, however most of them are limited by their use only in prokaryotes or only allow us to obtain snapshots of cellular events at a given time. Many efforts are ongoing to develop systems to test the order in which different events occur in a mammalian cell system to obtain a specific phenotype. Based on this, I set up an assay to verify a posteriori the order in which two different transcriptional events occur in a mammalian cell through a genome editing tool based on the CRISPR/Cas9 system. Artificial DNA t

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