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Littérature scientifique sur le sujet « DNA based species delimitation analysis »

Auteur : Grafiati

Publié le 7 juin 2025

Mis à jour le 2 août 2025

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Articles de revues sur le sujet "DNA based species delimitation analysis"

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Zhu, Xi-Chao, Jing Chen, Rui Chen, Li-Yun Jiang, and Ge-Xia Qiao. "DNA barcoding and species delimitation of Chaitophorinae (Hemiptera, Aphididae)." ZooKeys 656 (February 14, 2017): 25–50. https://doi.org/10.3897/zookeys.656.11440.

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Chaitophorinae aphids are widespread across Eurasia and North America, and include some important agricultural and horticultural pests. So, accurate rapid species identification is very important. Here, we used three mitochondrial genes and one endosymbiont gene to calculate and analyze the genetic distances within different datasets. For species delimitation, two distance-based methods were employed, threshold with NJ (neighbor-joining) and ABGD (Automatic Barcode Gap Discovery), and two tree-based approaches, GMYC (General Mixed Yule Coalescent) and PTP (Poisson Tree Process). The genetic in

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Song, Chao, Guanyu Chen, Le Wang, Teng Lei, and Xin Qi. "DNA Barcoding Supports “Color-Pattern’’-Based Species of Stictochironomus from China (Diptera: Chironomidae)." Insects 15, no. 3 (2024): 179. http://dx.doi.org/10.3390/insects15030179.

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The genus Stictochironomus (Diptera: Chironomidae) has an almost worldwide distribution, with more than 30 species. However, species delimitation and identification based on the markings on the wings and legs are controversial and uncertain. In this study, we focused on color patterns to review the adults of the genus from China, and two new species (S. trifuscipes sp. nov. and S. quadrimaculatus sp. nov.) are described and figured. DNA barcodes can accurately separate the two new species with specific color patterns. However, heterospecific individuals form a monophyletic cluster in the phylo

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Olave, Melisa, Eduard Solà, and L. Lacey Knowles. "Upstream Analyses Create Problems with DNA-Based Species Delimitation." Systematic Biology 63, no. 2 (2014): 263–71. http://dx.doi.org/10.1093/sysbio/syt106.

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Koohdar, Fahimeh, Neda Aram, and Masoud Sheidai. "Biosystematics, fingerprinting and DNA barcoding study of the genus Lallemantia based on SCoT and REMAP markers." Caryologia 74, no. 4 (2022): 77–83. http://dx.doi.org/10.36253/caryologia-1163.

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Lallemantia is a medicinally important plant in the world. Due to interspecific hybridization and horizontal gene transfer, spices relationship and delimitation on the genus Lallemantia is difficult based on different molecular markers. Therefore, selecting the appropriate marker can be important. Fingerprinting techniques continue to be used for genomic profiling for the characterization of germplasm and the establishment of the identity of varieties/hybrids/parental sources of aromatic and medicinal plants. For this, we need to produce detailed information on genetic diversity available in L

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Han, Wei, Lu Qiu, Jing Zhu, Zong-Qing Wang, and Yan-Li Che. "Exploring the diversity of Eupolyphaga Chopard, 1929 (Blattodea, Corydioidea): species delimitation based on morphology and molecular analysis." ZooKeys 1120 (September 5, 2022): 67–94. https://doi.org/10.3897/zookeys.1120.87483.

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Eupolyphaga Chopard, 1929 is a cockroach genus mainly endemic to China. In this study, the species diversity of this genus is further explored through morphology and molecular analysis. Four species are described new to science: Eupolyphaga miracidia Qiu, sp. nov., Eupolyphaga udenostyla Qiu, sp. nov., Eupolyphaga reducta Qiu, sp. nov., and Eupolyphaga simila Qiu, sp. nov. New knowledge on some known species is added, including new distribution records and characteristics of females. Forty-five COI sequences were newly sequenced and a molecular species delimitation analysis was performed using

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Silva, F. L., and S. Wiedenbrug. "Integrating DNA barcodes and morphology for species delimitation in theCorynoneuragroup (Diptera: Chironomidae: Orthocladiinae)." Bulletin of Entomological Research 104, no. 1 (2013): 65–78. http://dx.doi.org/10.1017/s0007485313000515.

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AbstractIn this study, we use DNA barcodes for species delimitation to solve taxonomic conflicts in 86 specimens of 14 species belonging to theCorynoneuragroup (Diptera: Chironomidae: Orthocladiinae), from the Atlantic Forest, Brazil. Molecular analysis of cytochrome c-oxidase subunit I (COI) gene sequences supported 14 cohesive species groups, of which two similar groups were subsequently associated with morphological variation at the pupal stage. Eleven species previously described based on morphological criteria were linked to DNA markers. Furthermore, there is the possibility that there ma

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TOMIMORI, YUSUKE, MASAFUMI MATSUI, HIROSHI OKAWA, KANTO NISHIKAWA, SHINGO TANABE, and RYO KAMASAKA. "Reassessment of species delimitation using nuclear markers in three lentic-breeding salamanders from the Chugoku District of Japan (Amphibia: Caudata: Hynobiidae)." Zootaxa 5293, no. 1 (2023): 145–60. http://dx.doi.org/10.11646/zootaxa.5293.1.6.

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Hynobius akiensis sensu lato has recently been split into three species based on short sequence analyses of cyt-b gene of mtDNA and without data of nuclear DNA, and strange sympatric distribution in some areas has been indicated in two species. We analyzed nuclear DNA marker (SNPs) and complete sequence of cyt-b in H. akiensis sensu lato to reassess species delimitation and genetic introgression among species. As a result, we found two lineages with discordant mitochondrial and nuclear DNA in some areas. Of H. akiensis sensu lato, each of the two contains the type locality of two species recen

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Van, Der Bank Herman, Dai Herbert, Richard Greenfield, and Kowiyou Yessoufou. "Revisiting species delimitation within the genus Oxystele using DNA barcoding approach." ZooKeys 365 (December 30, 2013): 337–54. https://doi.org/10.3897/zookeys.365.5356.

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The genus <i>Oxystele</i>, a member of the highly diverse marine gastropod superfamily Trochoidea, is endemic to southern Africa. Members of the genus include some of the most abundant molluscs on southern African shores and are important components of littoral biodiversity in rocky intertidal habitats. Species delimitation within the genus is still controversial, especially regarding the complex <i>O. impervia</i> / <i>O. variegata</i>. Here, we assessed species boundaries within the genus using DNA barcoding and phylogenetic tree reconstruction. We analysed 56 specimens using the mitochondri

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Becchimanzi, Andrea, Beata Zimowska, and Rosario Nicoletti. "Cryptic Diversity in Cladosporium cladosporioides Resulting from Sequence-Based Species Delimitation Analyses." Pathogens 10, no. 9 (2021): 1167. http://dx.doi.org/10.3390/pathogens10091167.

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Cladosporium cladosporioides is an extremely widespread fungus involved in associations ranging from mutualistic to pathogenic and is the most frequently represented Cladosporium species in sequence databases, such as Genbank. The taxonomy of Cladosporium species, currently based on the integration of molecular data with morphological and cultural characters, is in frequent need of revision. Hence, the recently developed species delimitation methods can be helpful to explore cryptic diversity in this genus. Considering a previous study that reported several hypothetical species within C. clado

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Ramirez, Jorge L., Luisa Simbine, Carla G. Marques, et al. "DNA Barcoding of Penaeidae (Decapoda; Crustacea): Non-Distance-Based Species Delimitation of the Most Economically Important Shrimp Family." Diversity 13, no. 10 (2021): 460. http://dx.doi.org/10.3390/d13100460.

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The Penaeidae family includes some of the most economic and ecological important marine shrimp, comprising hundreds of species. Despite this importance and diversity, the taxonomic classification for penaeid shrimp has constantly been revised, and issues related to the species identification are common. In this study, we implemented DNA barcoding analyses in addition to single-gene species delimitation analyses in order to identify molecular operational taxonomy units (MOTUs) and to generate robust molecular information for penaeid shrimp based on the cytochrome oxidase subunit I (COI) mitocho

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Thèses sur le sujet "DNA based species delimitation analysis"

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Wong, Evan S. "DNA-based Species Delimitation of the Agriculturally Important Genus, Ravinia (Diptera: Sarcophagidae)." University of Cincinnati / OhioLINK, 2015. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1439561953.

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Sholihah, Arni. "Diversification des biotas aquatiques de Sundaland : accumulation de la biodiversité chez les poissons d'eau douce et distribution dans un hotspot de biodiversité." Thesis, Montpellier, 2020. http://www.theses.fr/2020MONTG024.

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Sundaland est l'un des points chauds de biodiversité les plus menacés, en raison d’une augmentation rapide des menaces au cours des dernières décennies. Couvrant la péninsule malaise, Sumatra, Java et Bornéo, cette région possède l'une des plus grandes richesses en espèces et endémisme pour les vertébrés, y compris les poissons d'eau douce. Cette biodiversité a longtemps attiré l'attention des biologistes évolutionnistes, notamment en raison de la complexité de l'histoire géologique de Sundaland. Cette question a été abordée en explorant les patrons de fragmentation et dispersion durant la div

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Chang, Tsong-Chih, and 張聰智. "Analysis of genetic diversity of Ganoderma species based on ribosomal DNA internal transcribed spacer (ITS) region sequences and laccases gene." Thesis, 2011. http://ndltd.ncl.edu.tw/handle/88901216809281292868.

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碩士<br>國立中興大學<br>生命科學院碩士在職專班<br>99<br>With the rapid development of molecular biology techniques, the taxonomy of fungi has been transformed from by morphological phenotype into a new era of molecular classification. In this study, we use of polymerase chain reaction (PCR) technology to amplify both the consensus sequence of the laccase gene and the ribosomal DNA internal transcribed spacer sequences (ITS) of 15 Ganoderma strains (including four BCRC standard strains and 11 of wild type isolates) and three exceptionally genus strains. The specific 595~915 bp of PCR product of TS1-ITS2 fragemen

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Livres sur le sujet "DNA based species delimitation analysis"

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Taberlet, Pierre, Aurélie Bonin, Lucie Zinger, and Eric Coissac. Diet analysis. Oxford University Press, 2018. http://dx.doi.org/10.1093/oso/9780198767220.003.0017.

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Chapter 17 “Diet analysis” focuses on the specificities of DNA metabarcoding in the context of diet analyses. It presents the proof-of-concept study, which examined the diet of several herbivore species using next-generation sequencing for the first time. Chapter 17 reports how DNA-based analysis of bison diet helped evaluate the effect of conservation policies in a protected area. The different strategies aiming at disentangling predator and prey sequences in carnivorous diets are reviewed, including the use of a blocking oligonucleotide. Using bear diet as a study case, the issues associated

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Taberlet, Pierre, Aurélie Bonin, Lucie Zinger, and Eric Coissac. Environmental DNA. Oxford University Press, 2018. http://dx.doi.org/10.1093/oso/9780198767220.001.0001.

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Environmental DNA (eDNA), i.e. DNA released in the environment by any living form, represents a formidable opportunity to gather high-throughput and standard information on the distribution or feeding habits of species. It has therefore great potential for applications in ecology and biodiversity management. However, this research field is fast-moving, involves different areas of expertise and currently lacks standard approaches, which calls for an up-to-date and comprehensive synthesis. Environmental DNA for biodiversity research and monitoring covers current methods based on eDNA, with a par

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Taberlet, Pierre, Aurélie Bonin, Lucie Zinger, and Eric Coissac. Some early landmark studies. Oxford University Press, 2018. http://dx.doi.org/10.1093/oso/9780198767220.003.0011.

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Chapter 11 entitled “Some early landmark studies” revisits several seminal articles that paved the way for the field of eDNA research. It first evokes the paper that first coined the expression “environmental DNA” in the late 1980s. Then, it describes how eDNA was first exploited in the early 1990s to reveal an unsuspected microbial diversity that morphology- or cultivation-based methods had failed to reach. In the late 1990s, microbiologists began to explore in several pioneer papers the functional insight provided by “metagenomes” (i.e., the collective genomes found in eDNA samples). In the

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Taberlet, Pierre, Aurélie Bonin, Lucie Zinger, and Eric Coissac. Terrestrial ecosystems. Oxford University Press, 2018. http://dx.doi.org/10.1093/oso/9780198767220.003.0014.

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Chapter 14 “Terrestrial ecosystems” focuses on the use of eDNA analysis for the study of terrestrial organisms, especially those found in or associated with soil. While eDNA-based analyses have rapidly gained momentum in the freshwater ecology community, first for single-species detection and more recently for diversity surveys, their success has been less immediate among terrestrial ecologists. Soil microbiologists are a notable exception, as they quickly realized that targeting DNA directly in the environment could free them from cultivating microorganisms prior to any community census. This

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Chapitres de livres sur le sujet "DNA based species delimitation analysis"

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Malviya, Shubhra, and Sarita Agrawal. "RAPD-PCR-Based Genetic Relationships Among Three Forensically Important Calliphorid Species (Diptera: Calliphoridae)." In Proceedings of the Conference BioSangam 2022: Emerging Trends in Biotechnology (BIOSANGAM 2022). Atlantis Press International BV, 2022. http://dx.doi.org/10.2991/978-94-6463-020-6_24.

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AbstractMolecular genetic markers have been effectively used to analyze genetic relationships and diversity among different groups of dipterans. The emergence of Polymerase Chain Reaction (PCR) facilitated analysis of molecular markers e.g., Random Amplified Polymorphic DNA-Polymerase Chain Reaction (RAPD-PCR), has contributed a great deal in resolving the genetic relatedness in many dipterans of medical, veterinary, and economic importance. In the present study, an attempt has been made to explore the phylogenetic relationships among three calliphorid species, namely Hemipyrellia pulchra (Wei

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Gill, Bikram S. "A Century of Cytogenetic and Genome Analysis: Impact on Wheat Crop Improvement." In Wheat Improvement. Springer International Publishing, 2022. http://dx.doi.org/10.1007/978-3-030-90673-3_16.

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AbstractBeginning in the first decade of 1900, pioneering research in disease resistance and seed color inheritance established the scientific basis of Mendelian inheritance in wheat breeding. A series of breakthroughs in chromosome and genome analysis beginning in the 1920s and continuing into the twenty-first century have impacted wheat improvement. The application of meiotic chromosome pairing in the 1920s and plasmon analysis in the 1950s elucidated phylogeny of the Triticum-Aegilops complex of species and defined the wheat gene pools. The aneuploid stocks in the 1950s opened floodgates fo

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Oettel, Janine, Bettina Thalinger, Aglaia Szukala, Linus Munishi, and Katharina Lapin. "Monitoring Methods for the Protection of Connectivity in Forest Ecosystems." In Ecological Connectivity of Forest Ecosystems. Springer Nature Switzerland, 2025. https://doi.org/10.1007/978-3-031-82206-3_8.

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Abstract Forest ecosystems face increasing threats from climate change, resource exploitation, and other anthropogenic disturbances causing biodiversity loss and habitat fragmentation. The conservation priority of connected, healthy forests necessitates robust monitoring that covers the landscape, ecosystem, species, and genetic levels and employs direct as well as indirect methods. Connectivity objectives encompass patch colonization, prioritization, and landscape assessment at multiple scales. Monitoring landscapes and forest ecosystems involves assessing their physical attributes and functi

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Miyamoto, Michael M., and Joel Cracraft. "Phylogenetic Inference, DNA Sequence Analysis, and the Future of Molecular Systematics." In Phylogenetic analysis Of Dna sequences. Oxford University PressNew York, NY, 1991. http://dx.doi.org/10.1093/oso/9780195066982.003.0001.

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Abstract Comparative biology has had a long and noble history (Rieppel, 1988). Its central goal has been to understand the bewildering diversity of form observed across the world’s organisms. Concepts such as taxa (in particular, species) and homology have been the core intellectual instruments for facilitating this understanding. Thus, comparative biologists have labored to sort the world’s organisms into species (however construed), largely based on their characteristics of form (but viewed broadly, including any intrinsic attribute), and to describe their similarities and differences.

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García-Luque, Estefanía, Ana del Pino-Pérez, and Enrique Viguera. "Molecular Techniques for Analysis of Biodiversity by Agarose Gel Electrophoresis." In Electrophoresis - Recent Advances, New Perspectives and Applications [Working Title]. IntechOpen, 2023. http://dx.doi.org/10.5772/intechopen.1002268.

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Molecular techniques based on DNA analysis have become an indispensable tool for the identification and classification of organisms, addressing the limitations of taxonomy based on morphological characters. There are different methods for the analysis of the variability of DNA which can provide unique genetic signatures capable of distinguishing closely related species, hybrid specimens or even individuals within the same species. Here we describe two methods that allow species identification by agarose gel electrophoresis separation techniques. DNA barcoding is a method of identifying any spe

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Erlich, Henry A. "DNA of the Dead." In Genetic Reconstruction of the Past. Oxford University Press, 2023. http://dx.doi.org/10.1093/oso/9780197675366.003.0011.

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Abstract This chapter discusses the analysis of DNA sequences derived from extinct species, ancient human remains, and fossils of archaic hominim species, like the Neanderthals. It describes the work by Svante Paabo and his colleagues to sequence the Neanderthal genome and then to demonstrate that 2%–4% of most modern human genomes is Neanderthal in origin. It also discusses their work defining a previously unknown archaic hominim species, the Denisovans, based on the DNA sequence of a fingerbone found in a cave in Siberia. The chapter discusses the development of next generation sequencing te

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Meneely, Philip. "Genomes, chromosomes, and epigenetics." In Genetic Analysis. Oxford University Press, 2020. http://dx.doi.org/10.1093/hesc/9780198809906.003.0003.

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The genetic information for the biological processes that a species carries out is contained within the DNA sequence of its genome. The DNA sequences of many genomes having been completed, the next steps in genomic analysis involve annotation of the sequence, that is, identifying the functional elements within that sequence. These functional elements include such sequenced based information as transcripts, splice variants, and binding sites for proteins. While most of the attention in molecular genetics has been focused on the genes and their DNA sequences, the DNA in eukaryotic cells is packa

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Girman, Derek J. "The Use of PCR-Based Single-Stranded Conformation Polymorphism Analysis (PCR-SSCP) in Conservation Genetics." In Molecular Genetic Approaches in Conservation. Oxford University PressNew York, NY, 1996. http://dx.doi.org/10.1093/oso/9780195095265.003.0011.

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Abstract The use of molecular markers to investigate the population structure of endangered or threatened species has become a key component in the genetic management of endangered species (see chapter 1). The identification of variable genetic loci has allowed the analysis of DNA sequence data to become one of the most useful measures of genetic differentiation, genetic variability, and evolutionary related¬ ness for conservation-oriented analysis at or below the species level (Wayne and Jenks, 1991; Wenink et al. 1993; Gottelli et al., 1994; Rosel et al., 1994; Taberlet and Bouvet, 1994; Tay

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Kobel, H. R., C. Loumont, and R. C. Tinsley. "The extant species." In The Biology of Xenopus. Oxford University PressOxford, 1996. http://dx.doi.org/10.1093/oso/9780198549741.003.0002.

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Abstract As a result of recent detailed analysis of live specimens and also of sampling in more remote parts of Africa, the number of Xenopus species has trebled in the last 20 years. Studies of karyotype and DNA-content demonstrate a remarkable series of polyploid species. Based on two different chromosome sets of 10 and 18, they represent ploidy levels of 2:4:8:12. In fact, when compared with other members of the Pipidae, only a single species seems to have remained diploid. Other criteria used to define species are the mate calls, which in almost all cases are species-specific, and experime

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White, P. Scott, Owatha L. Tatum, Hakan Tegelstrom, and Llewellyn D. Densmore III. "Mitochondrial DNA isolation, separation, and detection of fragments." In Molecular Genetic Analysis of Populations. Oxford University PressOxford, 1998. http://dx.doi.org/10.1093/oso/9780199636341.003.0003.

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Abstract The 'revolution' that began in the 1980s with the application of mitochondrial DNA (mtDNA) analyses to address questions of molecular evolutionary and population biology has now largely become the paradigm for such work (1). Studies ranging in scope from purely systematic problems to identification and recognition of species to documentation of genetic changes in populations that have occurred over a very short period of time, are based on both indirect or direct estimates of nucleic acid variation. While progress continues to be made in analysing coding regions of nuclear DNA, the se

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Actes de conférences sur le sujet "DNA based species delimitation analysis"

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"DNA barcode-based delimitation of the Glossiphonia species." In SYSTEMS BIOLOGY AND BIOINFORMATICS. Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences, 2019. http://dx.doi.org/10.18699/sbb-2019-04.

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DeBry, Ronald W. "DNA-based species delimitation within the flesh fly genusRavinia(Diptera: Sarcophagidae)." In 2016 International Congress of Entomology. Entomological Society of America, 2016. http://dx.doi.org/10.1603/ice.2016.115691.

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Ulko, D. O., I. I. Gureyeva, R. S. Romanets, and A. A. Kuznetsov. "Molecular-phylogenetic analysis of the Cystopteridaceae family from North Asia based on plastid DNA loci." In Problems of studying the vegetation cover of Siberia. TSU Press, 2020. http://dx.doi.org/10.17223/978-5-94621-927-3-2020-40.

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The results of molecular phylogenetic studies based on the sequencing of matK gene and trnG-R intergenic spacer of the chloroplast DNA and analysis of the concatenated data of these loci of North Asian representatives of the Cystopteridaceae family are presented. In the genus Gymnocarpium, a dryopteris-clade was distinguished including species without glandular pubescence of fronds – Gymnocarpium dryopteris. Species having glandular pubescent fronds have formed the robertianum-clade (G. robertianum and G. fedtschenkoanum), and 2 subclades, including G. continentale, G. jessoense, and G. tenuip

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Кошкина, О. А., Т. Е. Денискова, А. Д. Соловьева, and Н. А. Зиновьева. "PHYLOGENETIC ANALYSIS OF RUSSIAN LOCAL SHEEP BREEDS BASED ON THE POLYMORPHISM OF THE MITOCHONDRIAL DNA CYTOCHROME B GENE." In Биотехнология в растениеводстве, животноводстве и сельскохозяйственной микробиологии. Crossref, 2021. http://dx.doi.org/10.48397/arriab.2021.21.xxi.030.

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Генетическое разнообразие популяций сельскохозяйственных животных играет жизненно важную роль в формировании признаков, ответственных за улучшение, выживание и адаптацию биологического вида [1]. Изучение генетической изменчивости современных аборигенных пород овец позволит накопить знания об их происхождении и расселении, а также понять влияние человеческой деятельности на овец с момента одомашнивания, что является актуальной задачей в современном мире. Одним из эффективных подходов к оценке генетического разнообразия является исследование митохондриальной ДНК (мтДНК). Филогенетический анализ

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Haque, Ainul, Ameeya Kumar Nayak, and Bernhard Weigand. "Electrokinetic Transport of Power Law Fluid Through a Micro-Channel With Hydrodynamic Slippage." In ASME-JSME-KSME 2019 8th Joint Fluids Engineering Conference. American Society of Mechanical Engineers, 2019. http://dx.doi.org/10.1115/ajkfluids2019-4848.

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Abstract A pressure driven electroosmotic flow (EOF) is numerically studied in a slit micro-channel with alternating hydrodynamic slip patches. The coupled Poisson-Boltzman-Navier Stokes equations dealt with external electric potential are solved for the flow enhancement of non-Newtonian fluids in microfluidic domain, which is a big challenge in the transportation and mixing of fluids in BioMEMS devices as the drag effect is very strong along the walls. This effect can be minimized with the use of slip boundary conditions by the coupling effects of liquid-liquid or gas-liquid interface positio

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Rapports d'organisations sur le sujet "DNA based species delimitation analysis"

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Reisch, Bruce, Pinhas Spiegel-Roy, Norman Weeden, Gozal Ben-Hayyim, and Jacques Beckmann. Genetic Analysis in vitis Using Molecular Markers. United States Department of Agriculture, 1995. http://dx.doi.org/10.32747/1995.7613014.bard.

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Genetic analysis and mapping in grapes has been difficult because of the long generation period and paucity of genetic markers. In the present work, chromosome linkage maps were developed with RAPD, RFLP and isozyme loci in interspecific hybrid cultivars, and RAPD markers were produced in a V. vinifera population. In three cultivars, there were 19 linkage groups as expected for a species with 38 somatic chromosomes. These maps were used to locate chromosome regions with linkages to important genes, including those influencing powdery mildew and botrytis bunch rot resistance; flower sex; and be

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Katz, Aron, Mark Johnson, and Jinelle Sperry. Environmental DNA sampling for at-risk and invasive species management on military ranges : guidelines and protocols for installation biologists and land managers. Engineer Research and Development Center (U.S.), 2024. http://dx.doi.org/10.21079/11681/48275.

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Environmental DNA (eDNA) analysis, or the detection of trace DNA shed by organisms into their environment, has the potential to transform Army capabilities for threatened and endangered species (TES) and invasive species management by providing a rapid, noninvasive, and cost-effective option for monitoring wildlife. Despite these benefits, eDNA analysis is underutilized on military installations as limited access to guidance materials, protocols, training opportunities, and support from eDNA scientists makes it difficult for installation biologists and military land managers to design and exec

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Freeman, Stanley, and Daniel Legard. Epidemiology and Etiology of Colletotrichum Species Causing Strawberry Diseases. United States Department of Agriculture, 2001. http://dx.doi.org/10.32747/2001.7695845.bard.

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Diseases caused by Colletotrichum spp. are one of the most important limitations on international strawberry production, affecting all vegetative and fruiting parts of the plant. From 1995 to 1997, C. acutatum infections reached epidemic levels in Israeli strawberry nurseries, causing extensive loss of transplants in fruit-bearing fields and additional reductions in yield. Although C. acutatum also occurs on strawberry in Florida, recent crown rot epidemics have been primarily caused by C. gloeosporioides. Little is known about the basic epidemiology of these important diseases on strawberry.

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Levin, Ilan, John W. Scott, Moshe Lapidot, and Moshe Reuveni. Fine mapping, functional analysis and pyramiding of genes controlling begomovirus resistance in tomato. United States Department of Agriculture, 2014. http://dx.doi.org/10.32747/2014.7594406.bard.

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Abstract. Tomato yellow leaf curl virus (TYLCV), a monopartitebegomovirus, is one of the most devastating viruses of cultivated tomatoes and poses increasing threat to tomato production worldwide. Because all accessions of the cultivated tomato are susceptible to these viruses, wild tomato species have become a valuable resource of resistance genes. QTL controlling resistance to TYLCV and other begomoviruses (Ty loci) were introgressed from several wild tomato species and mapped to the tomato genome. Additionally, a non-isogenic F₁diallel study demonstrated that several of these resistance sou

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Guelzow, Emma, Tanya Dapkey, and Jeff Smith. Freshwater snail inventory at Upper Delaware Scenic and Recreational River. National Park Service, 2025. https://doi.org/10.36967/2309764.

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The Upper Delaware Scenic and Recreational River (UPDE) unit of the National Park Service partnered with the Academy of Natural Sciences of Drexel University (ANS) to perform a freshwater snail inventory within the park. ANS and UPDE teams conducted field research in July and August of 2022 and 2023 using a combination of kick-net sampling and hand-picking methods to determine the abundance and diversity of freshwater snail populations. Fifty-four sites were surveyed along the 73 miles of the Delaware River within UPDE and included cold-water and warmwater fisheries zones. In 2022, field colle

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Levisohn, Sharon, Maricarmen Garcia, David Yogev, and Stanley Kleven. Targeted Molecular Typing of Pathogenic Avian Mycoplasmas. United States Department of Agriculture, 2006. http://dx.doi.org/10.32747/2006.7695853.bard.

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Intraspecies identification (DNA "fingerprinting") of pathogenic avian mycoplasmas is a powerful tool for epidemiological studies and monitoring strain identity. However the only widely method available for Mycoplasma gallisepticum (MG) and M. synoviae (MS)wasrandom amplified polymorphic DNA (RAPD). This project aimed to develop alternative and supplementary typing methods that will overcome the major constraints of RAPD, such as the need for isolation of the organism in pure culture and the lack of reproducibility intrinsic in the method. Our strategy focussed on recognition of molecular mark

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Tel-Zur, Neomi, and Jeffrey J. Doyle. Role of Polyploidy in Vine Cacti Speciation and Crop Domestication. United States Department of Agriculture, 2012. http://dx.doi.org/10.32747/2012.7697110.bard.

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1. Abstract: Over the past 25 years, vine cacti of the genera Hylocereus and Selenicereus have been introduced into Israel and southern California as new exotic fruit crops. The importance of these crops lies in their high water use efficiency and horticultural potential as exotic fruit crops. Our collaboration focused on the cytological, molecular and evolutionary aspects of vine cacti polyploidization to confront the agricultural challenge of genetic improvement, ultimately to improve success of vine cacti as commercial fruit crop plants. More specifically, we worked on the: 1- Identificatio

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Sittipraneed, Siriporn. การศึกษาการแพร่กระจายของประชากรผึ้งโพรง Apis cerana กลุ่มตอนเหนือและตอนใต้ในบริเวณพื้นที่รอยต่อโดยใช้ดีเอ็นเอเครื่องหมาย : รายงานผลการวิจัย. Chulalongkorn University, 2002. https://doi.org/10.58837/chula.res.2002.30.

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PCR-RFLP of three mtDNA regions ( srRNA gene ,irRNA gene and intergenic COI – COII region) were used to investigated the distribution of northern and southern Apis cerana populations of 89 colonies from Prachuap Khiri Khan and Chumphon provinces. Three, four and eight haplotypes were obtained from DraI digestion of PCR-amplified 410 bp srRNA gene, 755bp IrRNA gene and 1710 bp intergenic COI-COII region,respectively. These three mtDNA regions generated 11 composit haplotypes. Twelve composite haplotypes were generated when samples from Yunnan and Hanoi were included. A UPGMA phenogram based on

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Droby, Samir, Joseph W. Eckert, Shulamit Manulis, and Rajesh K. Mehra. Ecology, Population Dynamics and Genetic Diversity of Epiphytic Yeast Antagonists of Postharvest Diseases of Fruits. United States Department of Agriculture, 1994. http://dx.doi.org/10.32747/1994.7568777.bard.

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One of the emerging technologies is the use of microbial agents for the control of postharvest diseases of fruits and vegetables. A number of antagonistic microorganisms have been discovered which have the potential to effectively control postharvest diseases. Some of this technology has been patented and commercial products such as AspireTM (Ecogen Corporatin, Langhorne, PA, USA), Biosave 10TM and Biosave 11TM (Ecoscience Inc., Worchester, MA, USA) have been registered for commercial use. The principal investigator of this project was involved in developing the yeast-based biofungicide-Aspire

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