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Articles de revues sur le sujet « Dolichos Biflorus »

Auteur : Grafiati

Publié le 2 juin 2025

Mis à jour le 31 juillet 2025

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1

Gautam, Gundecha* Puja Bhangare Om Mane Vaishnavi Tomar Aditya Shimple Gourav Rokade. "Exploring the Anthelmintic Activity of Polyherbal Chocolates Formulated with Dolichos Biflorus and Lepidium Sativum Seed Extracts: An In-Vitro Evaluation." International Journal of Pharmaceutical Sciences 3, no. 5 (2025): 4286–97. https://doi.org/10.5281/zenodo.15515337.

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Helminthes infections are among the most common infections in human beings in which intestinal parasitic worms cause infection and physiological damage. Specially in children, intestinal parasite infections frequently result in morbidity and death. In the present study, we have investigated the anthelmintic activity of the Lepidium sativum and Dolichos biflorus, medicinal plant used for treatment of number of ailments. In addition, emphasis is given on formulation of polyherbal chocholate to increase palatability and compliance. Anthelmintic activity was evaluated on mature Pheretima Posthuma

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2

Bird, G. W. G. "The Dolichos biflorus and Peanut Lectins." Vox Sanguinis 56, no. 4 (1989): 288–89. http://dx.doi.org/10.1159/000460981.

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A, RAMAKRISHNAN, MUTHUKRISHNAN P, RANGANATHAN C.R, and SIVASAMY N. "Genetic Variability in Horsegram (Dolichos biflorus L.)." Madras Agricultural Journal 65, May (1987): 281–83. http://dx.doi.org/10.29321/maj.10.a03075.

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Genotypic coefficients of variation. heritability estimates and expected genetic advance were estimated for ten characters in eleven varieties of horsegram. All the characters except pod length and seeds per pod, showed higher heritability estimates. Number of pods per plant, number of nodules per plant, number of branches, plant height and yield per plant showed higher genetic gain values associated with higher heritability estimates. Therefore for effecting improvement through selection, number of pods per plant, number of nodules per plant, plant height and yield per plent should be conside

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Schnell, D. J., and M. E. Etzler. "Primary structure of the Dolichos biflorus seed lectin." Journal of Biological Chemistry 262, no. 15 (1987): 7220–25. http://dx.doi.org/10.1016/s0021-9258(18)48226-9.

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Alok, Shashi, Sanjay Kumar Jain, Amita Verma, and Mayank Kumar. "Pharmacognostic and phytochemical evaluation of Dolichos biflorus Linn." Asian Pacific Journal of Tropical Disease 4 (2014): S97—S101. http://dx.doi.org/10.1016/s2222-1808(14)60422-9.

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Jass, J. R., L. J. Allison, S. M. Stewart, and M. R. Lane. "Dolichos biflorus agglutinin binding in hereditary bowel cancer." Pathology 26, no. 2 (1994): 110–14. http://dx.doi.org/10.1080/00313029400169301.

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Suden, Akshay. "Managing Mutrashmari through Ayurvedic Drugs-I: Kulattha (Dolichos biflorus Linn)." Indian Journal of Ancient Medicine and Yoga 13, no. 1 (2020): 9–15. http://dx.doi.org/10.21088/ijamy.0974.6986.13120.1.

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Dao-Thi, Minh-Hoa, Thomas W. Hamelryck, Julie Bouckaert, et al. "Crystallization of two related lectins from the legume plant Dolichos biflorus." Acta Crystallographica Section D Biological Crystallography 54, no. 6 (1998): 1446–49. http://dx.doi.org/10.1107/s0907444998004478.

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The seed lectin DBL and the related stem and leaves lectin DB58 of the tropical legume Dolichos biflorus were crystallized, as well as complexes of DBL with adenine and with GalNAc(α1-3)[Fuc(α1-2)]Gal. The different crystal forms of DBL diffract to about 2.8 Å, while DB58 crystals diffract to 3.3 Å.

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Suralkar, AnupamaAshok, and SanjayB Kasture. "Anti-allergic and anti-anaphylactic activities of Dolichos biflorus." International Journal of Green Pharmacy 7, no. 3 (2013): 196. http://dx.doi.org/10.4103/0973-8258.120205.

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Natarajan, K. R. "Kinetic Study of the Enzyme Urease from Dolichos biflorus." Journal of Chemical Education 72, no. 6 (1995): 556. http://dx.doi.org/10.1021/ed072p556.

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B, Deepthi, Ashoka Shenoy M, and Karunakar Hegde. "Evaluation of hepatoprotective potential of methanolic extract of the plant dolichos biflorus linn." International Journal of Advances in Scientific Research 2, no. 5 (2016): 116. http://dx.doi.org/10.7439/ijasr.v2i5.3300.

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Present study is to evaluate the hepatoprotective potential of methanolic extract of the plant Dolichos biflorus Linn. against paracetamol and alcohol induced hepatotoxicity in rats. Oral administration of plant extract in two doses 200mg/kg and 400mg/kg body weight were subjected for the evaluation of hepatoprotective potential against alcohol (2ml/100g) and PCM (2g/kg) induced liver injury. Silymarin (25mg/kg) was used as a standard drug. The parameters like SGPT, SGO, ALP, TB and endogenous enzymes were estimated to assess the liver functions. In addition histopathological study was also ca

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12

Juhl, B. R. "Semiquantitative immunohistochemical evaluation of H-antigen expression in human ureters of different ABO- and Lewis types." Journal of Histochemistry & Cytochemistry 33, no. 9 (1985): 867–74. http://dx.doi.org/10.1177/33.9.3894503.

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A study was undertaken to obtain baseline information for future studies of H-antigen expression in urothelial tumors. The influence of ABO- and Lewis genes on H-antigen expression in human ureters was investigated. Thirty two ureters of different ABO- and Lewis types were stained by the Tween 20-modified immunoperoxidase staining technique using Ulex europaeus agglutinin I (32 cases) and two monoclonal anti-H antibodies (10 cases). Ureters from A and AB individuals were also stained using monoclonal anti-A and Dolichos biflorus agglutinin (14 cases). The H-antigen expression in endothelium of

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13

Quinn, Jeanette M., and Marilynn E. Etzler. "In Vivo Biosynthetic Studies of the Dolichos biflorus Seed Lectin." Plant Physiology 91, no. 4 (1989): 1382–86. http://dx.doi.org/10.1104/pp.91.4.1382.

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James, Douglas W., Mridul Ghosh, and Marilynn E. Etzler. "Production of a Lectin in Tissue Cultures of Dolichos biflorus." Plant Physiology 77, no. 3 (1985): 630–34. http://dx.doi.org/10.1104/pp.77.3.630.

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15

Saha, Sarmistha, and Ramtej J. Verma. "Efficacy Study of Dolichos biflorus in the Management of Nephrotoxicity." Asian Pacific Journal of Tropical Biomedicine 2, no. 3 (2012): S1471—S1476. http://dx.doi.org/10.1016/s2221-1691(12)60440-7.

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16

Renu Singh, Manoj Kumar Singh, Lovy Raj Chandra, et al. "In vitro Antioxidant and free radical scavenging activity of Macrotyloma uniflorum dal from Kumauni region." International Journal of Fundamental and Applied Sciences (IJFAS) 1, no. 1 (2012): 6–9. http://dx.doi.org/10.59415/ijfas.v1i1.19.

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The present study was carried out to evaluate the in vitro antioxidant activities of Methanolextract of Dolichos biflorus dal ((DME)) commonly edible food from central Himalayans. Methods: This wasachieved by screening of the plant extracts at varying concentrations (20-200μg/ml), using 2,2-diphenyl-1-picryl-hydrazyl (DPPH) radical scavenging activity, reducing power assay and hydrogen peroxide radical scavenging activity.Results: Total phenol and flavonoid contents (92.10 ± 8.11 mg/ml GAE per 100 mg plant extract and 139.5 ± 55.09mg/ml QE equivalent per 100 mg plant extract) were found respec

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Palanisamy, K., R. Pushpa, and M. Lenin. "Chromium (Cr) phyto-toxicity effect of Horse gram (Dolichos biflorus L.)." African Journal of Biological Sciences 02, no. 03 (2020): 94. http://dx.doi.org/10.33472/afjbs.2.3.2020.94-102.

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18

Saxena, Yogesh, Parth Sarthi, Brijesh Purwar, and Harsh Meena. "Dolichos biflorus Linn. ameliorates diabetic complications in streptozotocin induced diabetic rats." AYU (An International Quarterly Journal of Research in Ayurveda) 35, no. 4 (2014): 442. http://dx.doi.org/10.4103/0974-8520.159022.

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Gegg, Colin V., David D. Roberts, Irwin H. Segel, and Marilynn E. Etzler. "Characterization of the adenine binding sites of two Dolichos biflorus lectins." Biochemistry 31, no. 30 (1992): 6938–42. http://dx.doi.org/10.1021/bi00145a011.

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20

Rosenberg, Daniel W. "Non-homogeneous marking of distal colonic mucosa using Dolichos biflorus lectin." Cancer Letters 98, no. 1 (1995): 33–37. http://dx.doi.org/10.1016/s0304-3835(06)80007-8.

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21

Okada, Tomoko, Yasuyuki Imai, and Toshiaki Osawa. "In vivo localization of antitumor lymphocytes separated with Dolichos biflorus lectin." Immunology Letters 24, no. 3 (1990): 185–90. http://dx.doi.org/10.1016/0165-2478(90)90046-s.

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22

Schnell, D. J., K. Hori, S. M. Herrmann, C. V. Gegg, and M. E. Etzler. "Biosynthesis of DB58 Lectin in Cell Suspension Cultures of Dolichos biflorus." Archives of Biochemistry and Biophysics 310, no. 1 (1994): 229–35. http://dx.doi.org/10.1006/abbi.1994.1161.

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Rosenberg, D. "Non-homogeneous marking of distal colonic mucosa using Dolichos biflorus lectin." Cancer Letters 98, no. 1-2 (1995): 33–37. http://dx.doi.org/10.1016/0304-3835(95)03997-b.

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K., Palanisamy, Pushpa R., and Lenin M. "Chromium (Cr) phyto-toxicity effect of Horse gram (Dolichos biflorus L.)." African Journal of Biological Sciences 2, no. 3 (2020): 94–102. https://doi.org/10.33472/AFJBS.2.3.2020.94-102.

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In recent years, Heavy Metals (HMs) pollution has become a serious problem all over the world as these (HMs) continue in the soil for longer period due to their non-biodegradability. The chromium (Cr) is one of the nonessential heavy metal that makes highly toxicity in the plants as well as soil. Cr is one such nutrient required for sugar and fat metabolism in humans, whereas the role of Cr in plant growth and its uptake pathway are not yet fully understood. The pot experiments were carried out of the Horse gram (<em>Dolichos biflorus</em> L.) seeds were raised in pots, the pots containing 2.5

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25

Uiterdijk, H. G., B. A. J. Ponder, M. F. W. Festing, J. Hilgers, L. Skow, and R. Van Nie. "The gene controlling the binding sites of Dolichos biflorus agglutinin, Dlb-1, is on chromosome 11 of the mouse." Genetical Research 47, no. 2 (1986): 125–29. http://dx.doi.org/10.1017/s0016672300022953.

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SUMMARYA locus, Dlb-1, controlling the binding of the lectin from Dolichos biflorus to the intestinal epithelium and vascular endothelium of mice has been located on chromosome 11, 3.1 ± 1.4 centimorgans proximal to Rex, using recombinant inbred strains and conventional backcrosses with three-point linkage tests.The two alleles so far discovered at this locus behave unusually. The Dlb-1a allele causes binding to the vascular endothelium but not to the intestinal epithelium while Dlb-1b induces the exact reciprocal binding pattern, and the heterozygote shows both patterns.

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Furukawa, K., M. J. Mattes, and K. O. Lloyd. "A1 and A2 erythrocytes can be distinguished by reagents that do not detect structural differences between the two cell types." Journal of Immunology 135, no. 6 (1985): 4090–94. http://dx.doi.org/10.4049/jimmunol.135.6.4090.

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Abstract The differential reactivity of four mouse monoclonal antibodies (AbCB, AbHT29-36, AbM2, and AbS12) and Dolichos biflorus lectin with A1 and A2 erythrocytes was analyzed. Only AbS12 and D. biflorus lectin were able to preferentially agglutinate A1 erythrocytes. AbS12 is known to react only with short chain, unbranched structures (such as Aa-2 and Ab-2 glycolipids) and not with longer chains or with type 3 and type 4 structures. D. biflorus was shown to have a similar specificity by lectin staining of glycolipids separated by thin-layer chromatography. Analysis of the binding of radiola

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Li, Y. Q., S. A. Roberts, U. Paulus, M. Loeffler, and C. S. Potten. "The crypt cycle in mouse small intestinal epithelium." Journal of Cell Science 107, no. 12 (1994): 3271–79. http://dx.doi.org/10.1242/jcs.107.12.3271.

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We have used a mutation-induced marker system in the intestine of mice heterozygous at the Dlb-1 locus, which determines the expression of binding sites for the lectin Dolichos biflorus agglutinin, and the frequency of clustering of mutated crypts with time as a means of investigating the frequency of the crypt fission process and the crypt cycle. Whole-mount preparations from heterozygous Dlb-1b/Dlb-1a mice were stained with a peroxidase conjugate of Dolichos biflorus agglutinin. Mutations at the Dlb-1b locus in crypt stem cells result in loss of DBA-Px binding in these cells and subsequently

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Gelberg, Howard, Herbert Whiteley, Greg Ballard, John Scott, and Mark Kuhlenschmidt. "Temporal lectin histochemical characterization of porcine small intestine." American Journal of Veterinary Research 53, no. 10 (1992): 1873–80. http://dx.doi.org/10.2460/ajvr.1992.53.10.1873.

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SUMMARY A variety of biotinylated lectins was applied to formalin-fixed intestinal sections from isolator-reared pigs ranging in age from newborn through 12 weeks. Lectin binding to brush borders of villus enterocytes, crypt enterocytes, and dome epithelium, and lectin reactivity within goblet cells and Brunner's glands was semiquantified by microscopy and was used to estimate temporal changes in complex carbohydrates of enteric epithelium. Although variability in binding scores often was observed among pigs of the same age, several general patterns of lectin binding were detected. Dolichos bi

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SCHNELL, Danny J., Danny C. ALEXANDER, Bill G. WILLIAMS, and Marilynn E. ETZLER. "cDNA cloning and in vitro synthesis of the Dolichos biflorus seed lectin." European Journal of Biochemistry 167, no. 2 (1987): 227–31. http://dx.doi.org/10.1111/j.1432-1033.1987.tb13327.x.

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Gegg, C. V., and M. E. Etzler. "Photoaffinity labeling of the adenine binding sites of two Dolichos biflorus lectins." Journal of Biological Chemistry 269, no. 8 (1994): 5687–92. http://dx.doi.org/10.1016/s0021-9258(17)37515-4.

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Kumar, P. "Formation of Bowman–Birk inhibitors during the germination of horsegram (Dolichos biflorus)." Phytochemistry 60, no. 6 (2002): 581–88. http://dx.doi.org/10.1016/s0031-9422(02)00178-4.

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Zhou, Meng-Lan, Michael R. Frost, and Moon H. Nahm. "2597. Dolichos biflorus Agglutinin Binds to Pneumococcal Teichoic Acid and Lipoteichoic Acid." Open Forum Infectious Diseases 6, Supplement_2 (2019): S902—S903. http://dx.doi.org/10.1093/ofid/ofz360.2275.

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Abstract Background Dolichos biflorus agglutinin (DBA) is a lectin with a binding specificity toward α-linked N-acetylgalactosamine (α-GalNAc). While DBA is known to bind some, but not all, pneumococci, its target molecule has not been identified. Pneumococcus teichoic acid (TA) and lipoteichoic acid (LTA) have repeating units with α-GalNAc-(1→3)β-GalNAc decorated with phosphorylcholine (PC) at the O-6 positions. Two PC transferases, LicD1 and LicD2, mediate the attachment of PC to GalNAc residues while phosphorylcholine esterase (Pce) removes PCs attached to the terminal GalNAcs. We examined

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Quinn, Jeanette M., and Marilynn E. Etzler. "Isolation and characterization of a lectin from the roots of Dolichos biflorus." Archives of Biochemistry and Biophysics 258, no. 2 (1987): 535–44. http://dx.doi.org/10.1016/0003-9861(87)90375-4.

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Chao, Q. M., C. Casalongue, J. M. Quinn, and M. E. Etzler. "Expression and Partial Characterization of Dolichos biflorus Seed Lectin in Escherichia coli." Archives of Biochemistry and Biophysics 313, no. 2 (1994): 346–50. http://dx.doi.org/10.1006/abbi.1994.1397.

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Eguchi, M., T. Ozawa, J. Suda, K. Sugita, and T. Furukawa. "Lectins for electron microscopic distinction of eosinophils from other blood cells." Journal of Histochemistry & Cytochemistry 37, no. 5 (1989): 743–49. http://dx.doi.org/10.1177/37.5.2703708.

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Colloidal gold-labeled soybean agglutinin (SBA), Helix pomatia agglutinin (HPA), Dolichos biflorus agglutinin (DBA), and Griffonia simplicifolia lectin (GS-1) were used for electron microscopic observation of blood cells. Colloidal gold-labeled SBA, HPA, and DBA showed marked deposition on eosinophil granules at all stages of maturation. Gold particles were not deposited on basophils, neutrophils, monocytes, lymphocytes, or other blood cells. Only a few colloidal gold-labeled GS-1 were deposited on eosinophil granules. Eosinophil granules are rich in N-acetyl-D-galactosamine compounds, and the

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R, Ashvin, Praveen R, and Bagyaraj D.J. "Arbuscular mycorrhizal fungal association in legume roots and nodules." JOURNAL OF SOIL BIOLOGY AND ECOLOGY 43, no. 2 (2023): 38–42. http://dx.doi.org/10.58682/jsbe.43.2/uoeu3715.

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Legumes engage in tripartite symbiotic relationships with rhizobia and arbuscular mycorrhizal (AM) fungi. A study was conducted to know the direct association of AM fungi with nitrogen fixing rhizobium bacteroides, by determining the presence or absence of AM fungal hyphae or vesicles or arbuscules within the nodules. Eighteen different legumes were analysed for AM fungal colonization both in roots and nodules. Highest colonization rate was observed in Vigna unguiculata (84%) followed by Cicer arietinum (78%) and Rhynchosia heynei (75%). Least colonization rate was observed in Dolichos bifloru

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Chidrewar, G. U., V. S. Mane, M. M. Ghaisas, and A. D. Deshpande. "Dolichos biflorus Linn attenuate progression of renal damage in alloxan-induced diabetic rats." Oriental Pharmacy and Experimental Medicine 10, no. 2 (2010): 134–40. http://dx.doi.org/10.3742/opem.2010.10.2.134.

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Nigwekar, Ashok S., and Prakash D. Chavan. "The effect of water stress on nitrogen metabolism of horsegram Dolichos biflorus L." Acta Societatis Botanicorum Poloniae 59, no. 1-4 (2014): 73–80. http://dx.doi.org/10.5586/asbp.1990.007.

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Horsegram plants were raised in terule soil in earthen pots and subjected to various durations of water stress (7, 14 and 21 days). Total nitrogen contents were reduced in water-stressed plants but differently in different plant parts. Nitrate content and nitrate reductase activity decreased with stress. This species possesses a good capacity for accumulation of free proline under water stress. Analysis of amino acid composition also revealed marked changes in the levels of various amino acids in water-stressed plants. Accumulation of γ-aminobutyric acid in water-stressed plants was observed.

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Nakano, H. "Association of Dolichos biflorus lectin binding with full differentiation of bovine trophoblast cells." Reproduction 124, no. 4 (2002): 581–92. http://dx.doi.org/10.1530/reprod/124.4.581.

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Hormia, M., A. L. Kariniemi, L. Laitinen, and I. Virtanen. "Dolichos biflorus agglutinin (DBA) reacts selectively with mast cells in human connective tissues." Journal of Histochemistry & Cytochemistry 36, no. 10 (1988): 1231–37. http://dx.doi.org/10.1177/36.10.3138306.

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Dolichos biflorus agglutinin (DBA) binds to N-acetyl-D-galactosamine (GalNAc) residues in glycoconjugates and agglutinates erythrocytes carrying blood group antigen A. In cryostat sections of various tissues from blood group-specified humans, fluorochrome-coupled DBA bound preferentially to fusiform connective tissue cells and to certain epithelial cells. The connective tissue cells were identified as mast cells by their typical metachromasia in consecutive staining with toluidine blue. Double labeling with DBA and conjugated avidin revealed two distinct populations of mast cells. In several t

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Kuhar, Kalika, Rekha Kansal, Bhattiprolu Subrahmanyam, Kirpa Ram Koundal, Kanika Miglani, and Vijay Kumar Gupta. "A Bowman–Birk protease inhibitor with antifeedant and antifungal activity from Dolichos biflorus." Acta Physiologiae Plantarum 35, no. 6 (2013): 1887–903. http://dx.doi.org/10.1007/s11738-013-1227-8.

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Mathew, Limi Elizabeth, G. Sindhu, and A. Helen. "Dolichos biflorus exhibits anti-inflammatory and antioxidant properties in an acute inflammatory model." Journal of Food and Drug Analysis 22, no. 4 (2014): 455–62. http://dx.doi.org/10.1016/j.jfda.2014.02.002.

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Escada-Rebelo, S., A. F. Silva, S. Amaral, et al. "Spermatogonial stem cell organization in felid testis as revealed by Dolichos biflorus lectin." Andrology 4, no. 6 (2016): 1159–68. http://dx.doi.org/10.1111/andr.12223.

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Muricken, Deepa G., and Lalitha R. Gowda. "Functional expression of horsegram (Dolichos biflorus) Bowman–Birk inhibitor and its self-association." Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics 1804, no. 7 (2010): 1413–23. http://dx.doi.org/10.1016/j.bbapap.2010.02.012.

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Kirton, C. M., T. Wang, and G. J. Dockray. "Regulation of parietal cell migration by gastrin in the mouse." American Journal of Physiology-Gastrointestinal and Liver Physiology 283, no. 3 (2002): G787—G793. http://dx.doi.org/10.1152/ajpgi.00538.2001.

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Recent studies suggest that gastrin regulates parietal cell maturation. We asked whether it also regulates parietal cell life span and migration along the gland. Dividing cells were labeled with 5′-bromo-2′-deoxyuridine (BrdU), and parietal cells were identified by staining with Dolichos biflorus lectin. Cells positive for D. biflorus lectin and BrdU were reliably identified 10–30 days after BrdU injection in mice in which the gastrin gene had been deleted by homologous recombination (Gas-KO) and wild-type (C57BL/6) mice. The time course of labeling was similar in the two groups. The distribut

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Roopashree, Subbaiah, Sridevi Annapurna Singh, Lalitha R. Gowda, and A. G. Appu Rao. "Dual-function protein in plant defence: seed lectin from Dolichos biflorus (horse gram) exhibits lipoxygenase activity." Biochemical Journal 395, no. 3 (2006): 629–39. http://dx.doi.org/10.1042/bj20051889.

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Plant–pathogen interactions play a vital role in developing resistance to pests. Dolichos biflorus (horse gram), a leguminous pulse crop of the subtropics, exhibits amazing defence against attack by pests/pathogens. Investigations to locate the possible source of the indomitable pest resistance of D. biflorus, which is the richest source of LOX (lipoxygenase) activity, have led to a molecule that exhibits LOX-like functions. The LOX-like activity associated with the molecule, identified by its structure and stability to be a tetrameric lectin, was found to be unusual. The evidence for the lect

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Baishya, Mrinal. "Use of Kulattha (Dolichos biflorus Linn.) in the Management of Mutrashmari (Urinary Calculus): A Clinical Study." JOURNAL OF ADVANCED RESEARCH IN AYURVEDA, YOGA, UNANI, SIDHHA & HOMEOPATHY 11, no. 3&4 (2024): 1–6. https://doi.org/10.24321/2394.6547.202401.

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Ponder, Bruce A. J., Michael F. W. Festing, and Maureen M. Wilkinson. "An allelic difference determines reciprocal patterns of expression of binding sites for Dolichos biflorus lectin in inbred strains of mice." Development 87, no. 1 (1985): 229–39. http://dx.doi.org/10.1242/dev.87.1.229.

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We used staining of tissue sections by lectin conjugates to screen inbred strains of mice for polymorphisms which could be used as histological markers of chimaerism. We found one polymorphism, which involves reciprocal patterns of expression of binding sites for the N-acetyl-galactosamine- binding lectins from Dolichos biflorus (DBA), Helixpomatia (HPA) and Wisteria floribunda (WFA) on intestinal epithelium and vascular endothelium. The polymorphism is due to alleles at a single locus, designated Dlb-1 (for Dolichos lectin binding). Of 29 inbred strains examined, 3 are Dlb-la (type strain RHI

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Garg, Maryada, and Anoop K. Dobriyal. "Partial purification and characterisation of some low molecular weight á - amylases from Dolichos biflorus." Journal of Applied and Natural Science 3, no. 1 (2011): 75–77. http://dx.doi.org/10.31018/jans.v3i1.157.

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Dolichos biflorus, a commonly used legume in Uttarakhand, produces alpha amylase enzyme for conversion of starch present in its cotyledons to glucose, so that this glucose can be further utilized for the life controlling processes, glycolysis and Kreb’s cycle. Yield of this á - amylase isolated from the germinating legume comes out to be 27.7 IUml-1. Maximal amylase production occurs at pH 6.1 at 45O C. The enzyme was purified two fold, first with ultra-filtration and then with Ion-exchange chromatography. Ultra-filtration revealed size of amylase to be between 10 kDa and 30 kDa, against large

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Dvořánková, Barbora, Jan Motlík, Zuzana Holíková, Jiří Vacík, and Karel Smetana. "Dolichos biflorus agglutinin-binding site expression in basal keratinocytes is associated with cell differentiation." Biology of the Cell 94, no. 6 (2002): 365–73. http://dx.doi.org/10.1016/s0248-4900(02)00008-4.

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