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Littérature scientifique sur le sujet « Efficient reprogramming »

Auteur : Grafiati

Publié le 4 juin 2021

Mis à jour le 5 février 2022

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Articles de revues sur le sujet "Efficient reprogramming"

Gallego-Perez, Daniel, Jose J. Otero, Catherine Czeisler, Junyu Ma, Cristina Ortiz, Patrick Gygli, Fay Patsy Catacutan et al. « Deterministic transfection drives efficient nonviral reprogramming and uncovers reprogramming barriers ». Nanomedicine : Nanotechnology, Biology and Medicine 12, n^o 2 (février 2016) : 399–409. http://dx.doi.org/10.1016/j.nano.2015.11.015.

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Deng, Wenbin. « AID in reprogramming : Quick and efficient ». BioEssays 32, n^o 5 (14 avril 2010) : 385–87. http://dx.doi.org/10.1002/bies.201000014.

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Horna, David, Juan Carlos Ramírez, Anna Cifuentes, Antonio Bernad, Salvador Borrós et Manuel A. González. « Efficient Cell Reprogramming Using Bioengineered Surfaces ». Advanced Healthcare Materials 1, n^o 2 (16 février 2012) : 177–82. http://dx.doi.org/10.1002/adhm.201200017.

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Kang, Martin H., Jiabiao Hu, Richard E. Pratt, Conrad P. Hodgkinson, Aravind Asokan et Victor J. Dzau. « Optimizing delivery for efficient cardiac reprogramming ». Biochemical and Biophysical Research Communications 533, n^o 1 (novembre 2020) : 9–16. http://dx.doi.org/10.1016/j.bbrc.2020.08.104.

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Miller, Chris, et Christian Poellabauer. « Reliable and efficient reprogramming in sensor networks ». ACM Transactions on Sensor Networks 7, n^o 1 (août 2010) : 1–32. http://dx.doi.org/10.1145/1806895.1806901.

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Kulkarni, Sandeep, et Limin Wang. « Energy-efficient multihop reprogramming for sensor networks ». ACM Transactions on Sensor Networks 5, n^o 2 (mars 2009) : 1–40. http://dx.doi.org/10.1145/1498915.1498922.

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Hermann, Andreas, Jeong Beom Kim, Sumitra Srimasorn, Holm Zaehres, Peter Reinhardt, Hans R. Schöler et Alexander Storch. « Factor-Reduced Human Induced Pluripotent Stem Cells Efficiently Differentiate into Neurons Independent of the Number of Reprogramming Factors ». Stem Cells International 2016 (2016) : 1–6. http://dx.doi.org/10.1155/2016/4736159.

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Reprogramming of somatic cells into induced pluripotent stem cells (iPSCs) by overexpression of the transcription factors OCT4, SOX2, KLF4, and c-Myc holds great promise for the development of personalized cell replacement therapies. In an attempt to minimize the risk of chromosomal disruption and to simplify reprogramming, several studies demonstrated that a reduced set of reprogramming factors is sufficient to generate iPSC. We recently showed that a reduction of reprogramming factors in murine cells not only reduces reprogramming efficiency but also may worsen subsequent differentiation. To prove whether this is also true for human cells, we compared the efficiency of neuronal differentiation of iPSC generated from fetal human neural stem cells with either one (OCT4;hiPSC1F-NSC) or two (OCT4, KLF4;hiPSC2F-NSC) reprogramming factors with iPSC produced from human fibroblasts using three (hiPSC3F-FIB) or four reprogramming factors (hiPSC4F-FIB). After four weeks of coculture with PA6 stromal cells, neuronal differentiation ofhiPSC1F-NSCandhiPSC2F-NSCwas as efficient asiPSC3F-FIBoriPSC4F-FIB. We conclude that a reduction of reprogramming factors in human cells does reduce reprogramming efficiency but does not alter subsequent differentiation into neural lineages. This is of importance for the development of future application of iPSC in cell replacement therapies.

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Yu, Junying, Kevin Fongching Chau, Maxim A. Vodyanik, Jinlan Jiang et Yong Jiang. « Efficient Feeder-Free Episomal Reprogramming with Small Molecules ». PLoS ONE 6, n^o 3 (1 mars 2011) : e17557. http://dx.doi.org/10.1371/journal.pone.0017557.

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Hu, Kejin, Junying Yu, Kran Suknuntha, Shulan Tian, Karen Montgomery, Kyung-Dal Choi, Ron Stewart, James A. Thomson et Igor I. Slukvin. « Efficient generation of transgene-free induced pluripotent stem cells from normal and neoplastic bone marrow and cord blood mononuclear cells ». Blood 117, n^o 14 (7 avril 2011) : e109-e119. http://dx.doi.org/10.1182/blood-2010-07-298331.

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Abstract Reprogramming blood cells to induced pluripotent stem cells (iPSCs) provides a novel tool for modeling blood diseases in vitro. However, the well-known limitations of current reprogramming technologies include low efficiency, slow kinetics, and transgene integration and residual expression. In the present study, we have demonstrated that iPSCs free of transgene and vector sequences could be generated from human BM and CB mononuclear cells using nonintegrating episomal vectors. The reprogramming described here is up to 100 times more efficient, occurs 1-3 weeks faster compared with the reprogramming of fibroblasts, and does not require isolation of progenitors or multiple rounds of transfection. Blood-derived iPSC lines lacked rearrangements of IGH and TCR, indicating that their origin is non–B- or non–T-lymphoid cells. When cocultured on OP9, blood-derived iPSCs could be differentiated back to the blood cells, albeit with lower efficiency compared to fibroblast-derived iPSCs. We also generated transgene-free iPSCs from the BM of a patient with chronic myeloid leukemia (CML). CML iPSCs showed a unique complex chromosomal translocation identified in marrow sample while displaying typical embryonic stem cell phenotype and pluripotent differentiation potential. This approach provides an opportunity to explore banked normal and diseased CB and BM samples without the limitations associated with virus-based methods.

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Bussmann, Lars H., Alexis Schubert, Thien Phong Vu Manh, Luisa De Andres, Sabrina C. Desbordes, Maribel Parra, Timo Zimmermann et al. « A Robust and Highly Efficient Immune Cell Reprogramming System ». Cell Stem Cell 5, n^o 5 (novembre 2009) : 554–66. http://dx.doi.org/10.1016/j.stem.2009.10.004.

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Thèses sur le sujet "Efficient reprogramming"

Liu, Jing. « Reprogramming peripheral blood mononuclear cells using an efficient feeder-free, non-integration method to generate iPS cells and the effect of immunophenotype and epigenetic state on HSPC fate ». Thesis, University of Edinburgh, 2014. http://hdl.handle.net/1842/10031.

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Background and objectives: In 2006 Shinya Yamanaka successfully reprogrammed mouse fibroblasts back to an embryonic stem cell-like state (called induced pluripotent cells, iPS cells) using retrovirus to introduce four genes that encode critical transcription factor proteins (Oct4, Sox2, KLF4, and c-Myc). This ability to reprogram has promising future applications in clinical and biomedical research for study of diseases, development of candidate drugs and to support therapeutic treatments in regenerative medicine. However, the clinical applications have to meet GMP requirements without the risk of insertional mutagenesis associated with retrovirus. Chromatin modifying agents are widely used in many protocols to generate iPS cells and culture of blood CD34+ cells with chromatin-modifying agents can lead to an increase in marrow repopulating cells and in the case of valproic acid increased erythroid cell colony formation. We undertook research to help understand what effects these reagents have on mobilised peripheral blood (mPB) CD34+ cells and optimised the expansion medium protocol to facilitate reprogramming work. This project aims to utilize peripheral blood mononuclear cells (MNC), one of the most easily accessible tissues to generate iPS cells using an efficient non-viral, feeder cell free methodology, with the ultimate goal of moving this methodology towards clinical use. Materials and Methods: G-CSF mobilised peripheral blood, buffy coat, cord blood and fetal liver were obtained from patients and donors under informed consent and ethics committee approval. Haematopoietic stem/progenitor cells CD34+ or CD133+) isolated by magnetic separation were flow cytometry sorted into CD34+/CD133+, CD34+/CD133-, and CD34-/CD133+ sub-populations and their lineage potential were assessed in colony forming unit assays. The effect of epigenetic modifiers valproic acid and 5-aza-2-deoxycytidine used singly or in combination with each other and with IL3 on phenotype and lineage potential of cultured CD34+ cells from mobilised peripheral blood were assessed by flow cytometry and colony-forming unit assays. Prior to reprogramming mononuclear cells from peripheral blood or CD34+ cells from blood were expanded in culture medium supplemented with stem cell factor (SCF), Fms-related tyrosine kinase 3 ligand (Flt3L) and Interleukin- 3 (IL-3) for several days. Actively proliferating cells were reprogrammed by electroporation using episomal vectors with an oriP/EBNA-1 backbone to deliver five reprogramming genes, Oct4, Sox2, Lin28, L-Myc, and Klf4. Electroporated cells were seeded onto matrigel coated plates immediately after transfection or were reseeded after three days’ culture. Subsequently, cells were cultured in specific medium on different days. When iPS colonies appeared, they were picked and cultured as for ES cells. Once established, iPS cell lines were immunophenotyped using flow cytometry and immunofluorescence and their potential to differentiate into the three germ layers was assessed in vitro. Results and Conclusion: The largest subpopulation of CD34+ cells was CD34+/CD133+ population which was essentially committed to myeloid colony production, while much smaller CD34+/CD133- subpopulation had a greater capacity to generate erythroid colonies. Optimised cytokine cocktail for expansion of CD34+ cells included IL-3, important in improving expansion and maintaining functionality of CD34+ cells. The optimised cytokine cocktail comprised 100 ng/ml SCF, 10 ng/ml Flt3L, and 20 ng/ml IL-3, which maintained CD34+ cells and MNC in an active proliferating state. In addition, valproic acid and IL3 were found to act synergistically, to increase the numbers of CD34+/CD36+ positive cells. However, we found that an apparent increase in red cell colony formation actually resulted from a decrease in white cell colonies, so no overall increase in red cell colonies was seen when equivalent numbers of CD34+ cells were plated. Proliferating MNC maintained in optimised cytokine cocktail were amenable to electroporation for the effective delivery of episomal transcription factors (Oct4, Sox2, Klf4, L-Myc, and Lin28) within a backbone of oriP/EBNA-1. We successfully developed an efficient and simple method for reprogramming MNC from fresh or frozen samples to generate induced pluripotent cells using episomal vectors in a feeder-free system without any requirement for small molecules and the highest reprogramming efficiency is 0.033% (65 colonies from 2 ◊ 105 seeding MNC). The cytokine cocktail and reprogramming methods work better in CD34+ cells from cord blood or fetal liver, and we obtained 148 iPS colonies from 105 seeding cells (0.148%) at most. In addition, fibroblasts from adult and fetal liver can be successfully reprogrammed using the same reprogramming method. The use of episomal vectors with an oriP/EBNA-1 backbone to deliver reprogramming genes, and efficient electroporation were the most important factors in efficiency of the reprogramming process. In addition, it is pivotal to initiate transfection when cells are actively proliferating. The iPS cell lines we generated maintained the successful expression of ES markers including Oct4, Nanog, SSEA3. SSEA4, TRA-1-60 and TRA-1-81, and had the capacity to successfully differentiate into cell types of ectoderm, mesoderm and endoderm layers in vitro.

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Aston, Kenneth Ivan. « Indentification Of Factors Affecting Bovine Somatic Cell Nuclear Transfer Efficiency And Characterization Of Transciptional Profiles Of Nuclear Transfer Embyos and Cotyledons ». DigitalCommons@USU, 2007. https://digitalcommons.usu.edu/etd/87.

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Since the production of the first sheep by somatic cell nuclear transfer a great deal of effort has been made to improve efficiency and to understand nuclear reprogramming mechanisms. Unfortunately efficiency remains low, and nuclear reprogramming mechanisms remain uncharacterized. The objectives of this research were to identify factors associated with somatic cell nuclear transfer efficiency and to analyze the transcriptome of blastocyst-stage clone and control embryos and cotyledonary tissue in an effort to elucidate mechanisms responsible for the low developmental efficiency and high post-implantation losses. The experiments reported here identify factors including oocyte source and timing of activation following nuclear transfer that yield improved efficiencies. It was determined the use of cow oocytes for somatic cell nuclear transfer results in improved in vitro development and increased pregnancy rates. These data further indicate prolonged exposure of the donor nucleus to pre-activated oocyte cytoplasm results in increased nuclear fragmentation and reduced developmental efficiency in vitro. Several aberrantly expressed genes were identified in nuclear transfer blastocysts and cotyledons that could impact cloning efficiency. Major histocompatibility complex I and down-regulator of transcription 1 were overexpressed in nuclear transfer blastocysts, and retinol binding protein 1 was overexpressed in nuclear transfer cotyledons. The functions of these genes in immune response, transcriptional regulation, and retinol binding and transport make them attractive candidates for further nuclear transfer research. Expression levels of six developmentally important genes were analyzed in various stages of preimplantation nuclear transfer embryos by real-time polymerase chain reaction to determine the timing of nuclear reprogramming following nuclear transfer. Five of the six genes were aberrantly expressed multiple developmental stages, however by the blastocyst stage only one gene was aberrantly expressed. These data indicate reprogramming is delayed in nuclear transfer embryos resulting in over- or under-expression of developmentally important genes during early embryogenesis. These experiments report factors associated with improved nuclear transfer efficiency; provide insight into potential mechanisms for low developmental rates, abnormal placentation, and fetal loss of clones; and characterize the timing of nuclear reprogramming following somatic cell nuclear transfer.

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Hermann, Andreas, Jeong Beom Kim, Sumitra Srimasorn, Holm Zaehres, Peter Reinhardt, Hans R. Schöler et Alexander Storch. « Factor-Reduced Human Induced Pluripotent Stem Cells Efficiently Differentiate into Neurons Independent of the Number of Reprogramming Factors ». Saechsische Landesbibliothek- Staats- und Universitaetsbibliothek Dresden, 2016. http://nbn-resolving.de/urn:nbn:de:bsz:14-qucosa-203366.

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Reprogramming of somatic cells into induced pluripotent stem cells (iPSCs) by overexpression of the transcription factors OCT4, SOX2, KLF4, and c-Myc holds great promise for the development of personalized cell replacement therapies. In an attempt to minimize the risk of chromosomal disruption and to simplify reprogramming, several studies demonstrated that a reduced set of reprogramming factors is sufficient to generate iPSC. We recently showed that a reduction of reprogramming factors in murine cells not only reduces reprogramming efficiency but also may worsen subsequent differentiation. To prove whether this is also true for human cells, we compared the efficiency of neuronal differentiation of iPSC generated from fetal human neural stem cells with either one (OCT4; hiPSC1F-NSC) or two (OCT4, KLF4; hiPSC2F-NSC) reprogramming factors with iPSC produced from human fibroblasts using three (hiPSC3F-FIB) or four reprogramming factors (hiPSC4F-FIB). After four weeks of coculture with PA6 stromal cells, neuronal differentiation of hiPSC1F-NSC and hiPSC2F-NSC was as efficient as iPSC3F-FIB or iPSC4F-FIB. We conclude that a reduction of reprogramming factors in human cells does reduce reprogramming efficiency but does not alter subsequent differentiation into neural lineages. This is of importance for the development of future application of iPSC in cell replacement therapies.

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Preskey, David Alexander. « An mRNA-reprogramming method with improved kinetics and efficiency and the successful transdifferentiation of human fibroblasts using modified mRNA ». Thesis, University of Sheffield, 2017. http://etheses.whiterose.ac.uk/18242/.

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Induced pluripotent stem (iPS) cells have the potential to generate a wide array of cell types from multiple lineages that enable us to explore the mechanisms that are involved in the conversion of cell states. The reprogramming process that generates iPS cells is complex, but since its discovery, technical advancements and improvements in the methodology have improved the speed and efficiency of generating integration-free, clinically relevant iPS cells. However, despite improvements, the mechanisms of reprogramming are not fully understood and so the process remains largely inefficient and slow. It has been reported that reprogramming mediated through the delivery of exogenous mRNAs encoding OCT4, SOX2, KLF4 and cMYC is a fast and efficient method for generating integration-free iPS cells. Here we show that mRNA reprogramming can be enhanced further by employing an mRNA dose-ramping approach that provides greater control of the dose of mRNA that is introduced into the target cells. This improvement upon existing methods promotes the viability of the target cells during reprogramming which in turn improves the efficiency, speed and success of generating iPS cells. We also show that an optimisation to the reprogramming factor cocktail, replacing OCT4 with a fusion between OCT4 and the transcriptional activation domain of MYOD1 – called M3O, further improves the kinetics of reprogramming. Reprogramming disease cells is also possible in that several iPS cell-disease models have been established that have successfully modelled aspects of disease development in vitro. Here we show the applicability of using the mRNA approach we have developed, on neuroblastoma cells and the characterisation of iPS cells reprogrammed from neuroblastoma cells using OCT4, SOX2, KLF4 and cMYC delivered using Sendai viral vectors. Finally, we demonstrate how human fibroblasts introduced to a vector encoding MYOD1 causes them to transdifferentiate in to myoblast-like cells without a genomic footprint. Together this data demonstrates how integration-free mRNA can be used to control gene expression to direct cell fate through reprogramming and transdifferentiation. This mRNA approach provides proof of concept that warrants the testing of other genes to explore their function in reprogramming and other pathways that govern cell fate.

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Alves, Rita Alexandra Silvério. « Designing Transcription Factors for Efficient Hematopoietic Reprogramming ». Master's thesis, 2017. http://hdl.handle.net/10316/83092.

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Dissertação de Mestrado em Bioquímica apresentada à Faculdade de Ciências e Tecnologia
O transplante de células estaminais hematopoiéticas tem sido utilizado como tratamento para uma variedade de doenças hematológicas, devido à capacidade que as células estaminais hematopoiéticas possuem em se de autorrenovarem e se diferenciarem em todas as linhagens de células sanguíneas. No entanto, células em número insuficiente e incompatibilidades entre dadores e receptores dificultam a ampla aplicação desta terapia. Até à data, a expansão de células estaminais hematopoiéticas não foi possível e, por isso, estratégias adicionais para a geração deste tipo de células in vitro são necessárias, de modo a superar as limitações associadas ao transplante. A reprogramação de células somáticas mediada por fatores de transcrição abriu novas portas para a medicina regenerativa e permitiu o surgimento de abordagens para converter um tipo de célula diferenciada diretamente noutro. No sistema hematopoiético, a reprogramação direta de fibroblastos em células semelhantes a células estaminais hematopoiéticas foi obtida através da sobrexpressão dos fatores de transcrição Gata2, Gfi1b e cFos, fornecendo um método alternativo para gerar células autólogas para transplante, in vitro. Uma melhor compreensão do modo de ação destes três fatores, críticos durante a reprogramação, é necessária para aumentar a eficiência do processo.Aqui, eu defini possíveis domínios de reprogramação dos fatores de transcrição hematopoiéticos através da substituição desses mesmo fatores por genes homólogos (parálogos) ou versões com regiões deletadas, durante a reprogramação hematopoiética. Em primeiro lugar, os genes parálogos dos fatores Gata2, Gfi1b e cFos, e versões deletadas do fator Gata2 foram clonados num sistema lentiviral, de forma a direcionar a identidade celular de fibroblastos para a linhagem hematopoiética. Em segundo lugar, a eficiência da reprogramação hematopoiética foi avaliada através da ativação do repórter hCD34/H2BGFP. Curiosamente, Gata1 não substituiu Gata2 na reprogramação hematopoiética, apesar de algumas evidências apontarem para uma sobreposição de funções durante a hematopoiese. Não obstante, Gfi1b e cFos foram parcialmente substituídos por seus respectivos parálogos, indicando um papel determinante dos domínios não homólogos do Gata2 durante a reprogramação. Consistentemente, a reprogramação hematopoiética com versões deletadas do Gata2 revelou a necessidade dos domínios de transativação, do domínio regulatório negativo e do dedo de zinco do C-terminal do Gata2, para uma reprogramação bem-sucedida. Para mais, demonstrei que o Gata2 exibe atividade de marcação mitótica. Esta característica epigenética pode ser importante para a aquisição e manutenção da identidade celular das células estaminais hematopoiéticas, bem como na transmissão do estado celular reprogramado para as células filhas.No geral, este estudo identificou características funcionais dos fatores Gata2, Gfi1b e cFos, para a reprogramação e lança uma nova luz sobre como é adquirida e preservada a identidade celular das células estaminais hematopoiéticas. Daqui em diante, esses módulos de reprogramação serão críticos para o desenvolvimentos de fatores de transcrição/reprogramação melhorados, de modo a aumentar a eficiência de reprogramação hematopoiética, aproximando esta tecnologia da clínica.
Hematopoietic stem cell transplantation (HSCT) has been used as a treatment for a variety of haematological disorders, due to the ability of hematopoietic stem cells (HSCs) to self-renew and differentiate into all blood cell lineages. Insufficient number of cells and matching incompatibilities between donors and recipients hinder the broad application of this therapy. Expansion of HSCs has met limited success and additional strategies for the in vitro generation of HSCs are required to overcome transplant-associated limitations. Somatic cell reprogramming mediated by transcription factors (TFs) is opening new avenues for regenerative medicine and allowed the design of new approaches to convert one differentiated cell type directly into another. In the hematopoietic system, direct reprogramming of fibroblasts to HSC-like cells has been shown through ectopic expression of Gata2, Gfi1b and cFos, providing an alternative method to generate patient tailored HSCs in vitro. A better understanding of the mode of action of these three critical TFs during reprogramming is needed in order to increase the efficiency of the process.Here, I have defined potential reprogramming domains of hematopoietic TFs by homologous gene (paralog) and deletion construct substitution during hematopoietic reprogramming. First, paralogs of Gata2, Gfi1b and cFos and Gata2 deletion constructs were cloned into a lentiviral gene delivery system to induce fibroblast cell identity towards the hematopoietic lineage. Secondly, hematopoietic reprogramming efficiency was assessed by hCD34/H2BGFP reporter activation. Interestingly, Gata1 did not substitute Gata2 for hematopoietic reprogramming, despite evidences of overlapping function during hematopoiesis. Notwithstanding, Gfi1b and cFos were partially replaced by their respective paralogs, indicating a determinant role for non-homologous domains of Gata2 during reprogramming. Consistently, hematopoietic reprogramming with Gata2 deletion constructs revealed the requirement of the transactivation domains (TADs), the negative regulatory domain (NRD) and the C-terminal zinc finger (C-ZF) for successful reprogramming. Remarkably, I have also unveiled that Gata2 display mitotic bookmarking activity. This epigenetic feature may be important for the acquisition and maintenance of the HSC fate as well as the inheritance of the reprogrammed cell state to daughter cells. Overall, this study identified functional reprogramming features of Gata2, Gfi1b and cFos and sheds new light on how the HSC fate is acquired and preserved. Hereafter, these reprogramming modules will be critical for the design of enhanced synthetic TFs to increase hematopoietic reprogramming efficiency bringing this technology one step closer to clinical translation.

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Wang, Yen-Cheng, et 王彥程. « An Efficient Dynamic Reprogramming Mechanism for Wireless Sensor Networks ». Thesis, 2006. http://ndltd.ncl.edu.tw/handle/20517298165245979340.

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碩士
中興大學
資訊科學系所
94
With the progress of Micro-electromechanical System (MEMS) and wireless communication technology, a tiny device can equip with microprocessor, wireless communication and sensor. Such a tiny device is called a sensor node. The wireless sensor network can be used in extensively fields and attract many researcher perform the research on this field. However, the wireless sensor network still be subjected to some restrictions. For example, the limited memory space can''t store much of data and the application programs. In addition, sensor nodes often use battery as the power source, which may have the problem of power shortage. Finally, once deployed, it is difficult to reprogram the sensor nodes. As a result, in this thesis, we present a mechanism to wirelessly reprogram the user applications in a sensor node. By our proposed architecture, the system designer can efficiently add, remove and update the application modules. Furthermore, the reprogramming procedure is an on-line process and has no impact on the normal operating of sensor node. In addition, since the extensively energy constraint in the sensor network, we divide the function of a linking loader into two parts. A sensor node only needs to perform the loading process while the linking process is pre-processed on the server to save the energy consumption of a sensor node. From the experimental result, our scheme can save 14% memory usage compared to SOS. Furthermore, the application module execution speed of our scheme is 20 % faster than SOS.

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SHAFI, NASIF BIN. « Efficient Over-the-air Remote Reprogramming of Wireless Sensor Networks ». Thesis, 2011. http://hdl.handle.net/1974/6890.

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Over-the-air reprogramming is an important aspect of managing large wireless sensor networks. However, reprogramming deployed sensor networks poses significant challenges due to the energy, processing power and memory limitation of sensor nodes. For improved energy efficiency, a reprogramming mechanism should use less transmission and flash writing overhead. Past research has proposed different mechanisms for reprogramming deployed sensor networks. However, all of these mechanisms produce large patches if software modifications involve changing program layouts and shifting global variables. In addition, existing mechanisms use large amounts of external flash and rewrite entire internal flash. In this thesis, we present a differential reprogramming mechanism called QDiff that mitigates the effects of program layout modifications and retains maximum similarity between old and new software using a clone detection mechanism. Moreover, QDiff organizes the global variables in a novel way that eliminates the effect of variable shifting. Our experiments show that QDiff requires near-zero external flash, and significantly lower internal flash rewriting and transmission overhead than leading existing differential reprogramming mechanisms.
Thesis (Master, Computing) -- Queen's University, 2011-11-29 14:11:44.138

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Chang, Ching-Hsiang, et 張景翔. « Enhancement of Pichia pastoris AOX1 Promoter Efficiency by Reprogramming the Transcription Factor Mxr1 ». Thesis, 2017. http://ndltd.ncl.edu.tw/handle/tqcyfr.

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碩士
國立臺灣大學
生化科技學系
105
The methylotrophic yeast Pichia pastrois has been extensively applied in production of recombinant proteins because it combines the advantages of single cell in microbial and post-translational modification in eukaryotic systems. The AOX1 promoter (PAOX1) is the most common promoter used for heterologous protein expression in P. pastoris. A glycerol-methanol-shift induction strategy is applied to achieve high productivity. However, the tightly regulated PAOX1 also led P. pastoris expression to restrictive conditions. To improve the efficiency of protein production, we tried to reprogram the transcriptional regulation of PAOX1 in P. pastoris. The ectopic Mxr1 expressed by the mild AOX2 promoter (PAOX2) did not cause growth defect. The transcriptional efficiency of PAOX1 was enhanced since the limitation of Mxr1 titration effect was broken by extra Mxr1. PAOX1 became more flexible due to the positive feedback of Mxr1 and was regulated by glycerol. With the extra Mxr1 driven by PAOX2, PAOX1 showed better activity without than that with medium replacement. Moreover, glycerol starvation induced GFP production with reprogramming Mxr1 in P. pastoris. Increasing copy number of ectopic Mxr1 did not enhanced the efficeince of PAOX1. These results showed overexpression of Mxr1 by one copy of PAOX2 might be enough to achieve the maximum activity of PAOX1. Although the improvement of transcriptional efficiency might be limited by secretory ability, these problems could be sloved by combination with other strategies. In conclusion, transcriptional reprogramming of Mxr1 improved the efficiency of P. pastrois under methanol induction and potentially made P. pastrois become methanol-free induction system to eliminate the problems of methanol.

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Chapitres de livres sur le sujet "Efficient reprogramming"

Zhao, Xiaoyang. « Establishment of Highly Efficient Somatic Cell Reprogramming System to Generate iPSC Lines ». Dans Studies of Pluripotency in Embryonic Stem Cells and Induced Pluripotent Stem Cells, 41–52. Dordrecht : Springer Netherlands, 2014. http://dx.doi.org/10.1007/978-94-017-8819-9_3.

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Wang, Bibo, Yu Chen, Hongliang Gu, Jian Yang et Tan Zhao. « Two Energy-Efficient, Timesaving Improvement Mechanisms of Network Reprogramming in Wireless Sensor Network ». Dans Embedded Software and Systems, 473–83. Berlin, Heidelberg : Springer Berlin Heidelberg, 2005. http://dx.doi.org/10.1007/11599555_45.

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McGrath, Patrick S., Shennea S. McGarvey, Igor Kogut et Ganna Bilousova. « Efficient RNA-Based Reprogramming of Disease-Associated Primary Human Fibroblasts into Induced Pluripotent Stem Cells ». Dans Methods in Molecular Biology, 271–84. New York, NY : Springer US, 2020. http://dx.doi.org/10.1007/978-1-0716-0301-7_17.

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Cevallos, Ricardo Raúl, Md Emon Hossain, Ruowen Zhang et Kejin Hu. « Evaluating Reprogramming Efficiency and Pluripotency of the Established Human iPSCS by Pluripotency Markers ». Dans Methods in Molecular Biology, 235–49. New York, NY : Springer US, 2020. http://dx.doi.org/10.1007/978-1-0716-1084-8_15.

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Sean, Stefano Rollo, Emanuel Popovici et Brendan Ofly. « Energy-efficient Reprogramming of Heterogeneous Wireless Sensor Networks ». Dans Sustainable Wireless Sensor Networks. InTech, 2010. http://dx.doi.org/10.5772/13813.

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Actes de conférences sur le sujet "Efficient reprogramming"

Tan, Jin, JinTan Chen et YanQiu Liu. « An Efficient Authentication Strategy for Reprogramming of Sensor Networks ». Dans 2007 International Conference on Computational Intelligence and Security (CIS 2007). IEEE, 2007. http://dx.doi.org/10.1109/cis.2007.227.

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Gu, Qijun. « Efficient code diversification for network reprogramming in sensor networks ». Dans the third ACM conference. New York, New York, USA : ACM Press, 2010. http://dx.doi.org/10.1145/1741866.1741890.

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Tsiftes, Nicolas, Adam Dunkels et Thiemo Voigt. « Efficient Sensor Network Reprogramming through Compression of Executable Modules ». Dans 2008 5th Annual IEEE Communications Society Conference on Sensor, Mesh and Ad Hoc Communications and Networks. IEEE, 2008. http://dx.doi.org/10.1109/sahcn.2008.51.

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Stolikj, Milosh, Pieter J. L. Cuijpers et Johan J. Lukkien. « Efficient reprogramming of wireless sensor networks using incremental updates ». Dans 2013 IEEE International Conference on Pervasive Computing and Communications Workshops (PerCom Workshops 2013). IEEE, 2013. http://dx.doi.org/10.1109/percomw.2013.6529563.

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De, Pradip, Yonghe Liu et Sajal K. Das. « ReMo : An Energy Efficient Reprogramming Protocol for Mobile Sensor Networks ». Dans 2008 IEEE International Conference on Pervasive Computing and Communications (PerCom). IEEE, 2008. http://dx.doi.org/10.1109/percom.2008.89.

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Mazumder, Biswajit, et Jason O. Hallstrom. « An efficient code update solution for wireless sensor network reprogramming ». Dans 2013 International Conference on Embedded Software (EMSOFT). IEEE, 2013. http://dx.doi.org/10.1109/emsoft.2013.6658582.

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Dong, Wei, Biyuan Mo, Chao Huang, Yunhao Liu et Chun Chen. « R3 : Optimizing relocatable code for efficient reprogramming in networked embedded systems ». Dans IEEE INFOCOM 2013 - IEEE Conference on Computer Communications. IEEE, 2013. http://dx.doi.org/10.1109/infcom.2013.6566786.

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Kim, Seung-Ku, Jae-Ho Lee, Kyeong Hur et Doo-Seop Eom. « Tiny Function-Linking for Energy-Efficient Reprogramming in Wireless Sensor Networks ». Dans 2009 Third International Conference on Mobile Ubiquitous Computing, Systems, Services and Technologies (UBICOMM). IEEE, 2009. http://dx.doi.org/10.1109/ubicomm.2009.22.

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Lei Yang, Zhang Xiao-Yi et Wang Xiao-Mei. « RECD : a reliable and efficient code distribution protocol for network reprogramming ». Dans IET Conference on Wireless, Mobile and Sensor Networks 2007 (CCWMSN07). IEE, 2007. http://dx.doi.org/10.1049/cp:20070148.

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Xing Liu, Kun Mean Hou, Hongling Shi, Chengcheng Guo et Haiying Zhou. « Efficient and portable reprogramming method for high resource-constraint wireless sensor nodes ». Dans 2011 IEEE 7th International Conference on Wireless and Mobile Computing, Networking and Communications (WiMob). IEEE, 2011. http://dx.doi.org/10.1109/wimob.2011.6085359.

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