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Littérature scientifique sur le sujet « Enterococcus faecali »

Auteur : Grafiati

Publié le 9 mars 2023

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Articles de revues sur le sujet "Enterococcus faecali"

Maheux, Andrée F., Sébastien Bouchard, Ève Bérubé, and Michel G. Bergeron. "Rapid molecular identification of fecal origin-colonies growing on Enterococcus spp.-specific culture methods." Journal of Water and Health 15, no. 2 (2016): 239–50. http://dx.doi.org/10.2166/wh.2016.199.

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The mEI, Chromocult® enterococci, and m-Enterococcus culture-based methods used to assess water quality by the detection of Enterococcus spp. were first compared in terms of sensitivity using (1) 41 different type strains of Enterococcus spp. and (2) environmental colonies identified by 16S rRNA sequencing. Then, two specific-rtPCR assays targeting Enterococcus spp. and Enterococcus faecalis/faecium were tested for their ability to confirm the identity of putative enterococcal colonies. The mEI, Chromocult® enterococci, and m-Enterococcus methods detected β-glucosidase activity for 28 (68.3%), 32 (78.0%), and 12 (29.3%) of the 41 reference enterococcal strains tested, respectively. Analysis with environmental colonies showed that mEI and Chromocult® enterococci media had false positive rates of 4.3% and 5.0%, respectively. Finally, the two rtPCR assays showed a specificity of 100%. Only two (2/19) colonies of E. faecium isolated from mEI agar were not detected by the Enterococcus faecium rtPCR assay, for a sensitivity of 89.5%. Our results showed that Chromocult® enterococci medium recovered more E. faecalis/faecium cells than the two other methods. Thus, the use of Chromocult® enterococci combined with the Enterococcus faecalis/faecium rtPCR assay showed the best combination to decrease the high false-positive rate obtained when the entire Enterococcus genus is targeted.

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Kumurya, A. S., and B. Ega. "An Overview on Vancomycin Resistant Enterococcus faecalis." UMYU Journal of Microbiology Research (UJMR) 6, no. 1 (2021): 160–67. http://dx.doi.org/10.47430/ujmr.2161.033.

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There are over 15 species of the Enterococcus genus, 80-90% of clinical isolates as E. faecalis. The aim of this work is to review the current information on Vancomycin resistant Enterococcus fecalis. The study reviewed using electronic documents and hard copies from public libraries of relevant literatures relating to biology, epidemiology, drug resistance mechanism, treatment, and control of Enterococcus faecalis. The review revealed that Enterocuccus faecalis formerly known as Streptococcus faecalis is a Gram-positive commensal bacterium that inhabits the gastrointestinal tracts of healthy humans and other mammals. However, it can cause lifethreatening infections in humans, especially in the nosocomial environment, where there are naturally high levels of antibiotic resistance. Thus, Enterococci have proven to present a therapeutic challenge because of their resistance to many antimicrobial drugs, including cell-wall active agents; aminoglycosides, penicillin, ampicillin, and vancomycin.” The Enterococci have the capacity to acquire a wide variety of antimicrobial resistance factors through plasmid transfer by conjugation, which present serious problems in the management of patients with Enterococcal infections. In general, Enterococcal isolates with lowered susceptibility to vancomycin are categorized as vanA, vanB, and vanC, vanA and vanB pose the greatest threat because they are the most resistant genes.E. faecalis are also resistant to teicoplanin. Enterococcal strains that are vancomycin-dependent have been found, but are rare and less common than vancomycin-resistant strains (referred to as “vancomycin-resistant Enterococci” or “VRE”). The review, identified that although VRE infection possess the tendency to become endemic especially in very ill debilitated patients who have been exposed to broad spectrum antibiotics; and the immune-compromised, yet Vancomycin continues to be the drug of choice for serious life threatening infections as sepsis, pneumonia, and endocarditis. Keywords: Vancomycin-resistant Enterococci(VRE), Enterococcus faecalis, Resistance gene

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Cox, Christopher R., and Michael S. Gilmore. "Native Microbial Colonization of Drosophila melanogaster and Its Use as a Model of Enterococcus faecalis Pathogenesis." Infection and Immunity 75, no. 4 (2007): 1565–76. http://dx.doi.org/10.1128/iai.01496-06.

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ABSTRACT Enterococci are commensal organisms of the gastrointestinal (GI) tracts of a broad range of mammalian and insect hosts, but they are also leading causes of nosocomial infection. Little is known about the ecological role of enterococci in the GI tract consortia. To develop a tractable model for studying the roles of these organisms as commensals and pathogens, we characterized the Drosophila melanogaster microflora and examined the occurrence of enterococci in the gastrointestinal consortium of Drosophila. In a survey of laboratory-reared Drosophila and wild-captured flies, we found that Drosophila was naturally colonized by representatives of five bacterial phyla. Among these organisms were several species of enterococci, including Enterococcus faecalis, Enterococcus faecium, Enterococcus gallinaraum, and Enterococcus durans, as well as a previously detected but uncultured Enterococcus species. Drosophila could be cured of enterococcal carriage by antibiotic treatment and could be reassociated with laboratory strains. High-level colonization by a well-characterized strain expressing the enterococcal cytolysin was found to be detrimental to Drosophila compared to the effect of an isogenic, noncytolytic control. The anatomical distribution of enterococci in the Drosophila GI tract was determined by immunohistochemical staining of thin sections of naturally colonized and reassociated flies.

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Lauková, Andrea, Viola Strompfová, Jana Ščerbová, and Monika Pogány Simonová. "Virulence Factor Genes Incidence among Enterococci from Sewage Sludge in Eastern Slovakia following Safety Aspect." BioMed Research International 2019 (October 7, 2019): 1–5. http://dx.doi.org/10.1155/2019/2735895.

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The sewage sludges represent a potential health hazard because of the quantity of different microbiota detected in sewages. Among microbiota detected in sewages, also belong representatives of the phylum Firmicutes. In the past, environmental enterococci in addition to coliforms were widely used as indicators of faecal contamination. Regarding the enterococcal strains as potential pathogenic bacteria, their pathogenicity is mainly caused by production of virulence factors. Therefore, the aim of the study was to analyse incidence of virulence factors in enterococci from cows' dung water. Species identification of 24 enterococci using MALDI-TOF MS system allotted 23 strains to the species Enterococcus faecium with highly probable species identification and E. faecalis EEV20 with a score value meaning secure genus identification/probable species identification. Enterococci were absent of cytolysin A gene, hyaluronidase gene, and element IS gene. It can be concluded that they are not invasive which is very important from safety aspect. The most frequently detected gene was adhesin E. faecium (efaAfm, in 22 E. faecium strains and in one E. faecalis). Adhesin efaAfs gene was detected in E. faecalis EEV20 and in two E. faecium. GelE gene was present in three strains. E. faecium EF/EC31 was absent of virulence factor genes.

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Conwell, Michael, James S. G. Dooley, and Patrick J. Naughton. "A Novel Biofilm Model System to Visualise Conjugal Transfer of Vancomycin Resistance by Environmental Enterococci." Microorganisms 9, no. 4 (2021): 789. http://dx.doi.org/10.3390/microorganisms9040789.

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Enterococci and biofilm-associated infections are a growing problem worldwide, given the rise in antibiotic resistance in environmental and clinical settings. The increasing incidence of antibiotic resistance and its propagation potential within enterococcal biofilm is a concern. This requires a deeper understanding of how enterococcal biofilm develops, and how antibiotic resistance transfer takes place in these biofilms. Enterococcal biofilm assays, incorporating the study of antibiotic resistance transfer, require a system which can accommodate non-destructive, real-time experimentation. We adapted a Gene Frame® combined with fluorescence microscopy as a novel non-destructive platform to study the conjugal transfer of vancomycin resistance in an established enterococcal biofilm.A multi-purpose fluorescent in situ hybridisation (FISH) probe, in a novel application, allowed the identification of low copy number mobile elements in the biofilm. Furthermore, a Hoechst stain and ENU 1470 FISH probe identified Enterococcus faecium transconjugants by excluding Enterococcus faecalis MF06036 donors. Biofilm created with a rifampicin resistant E. faecalis (MW01105Rif) recipient had a transfer efficiency of 2.01 × 10−3; double that of the biofilm primarily created by the donor (E. faecalis MF06036). Conjugation in the mixed enterococcal biofilm was triple the efficiency of donor biofilm. Double antibiotic treatment plus lysozyme combined with live/dead imaging provided fluorescent micrographs identifying de novo enterococcal vancomycin resistant transconjugants inside the biofilm. This is a model system for the further study of antibiotic resistance transfer events in enterococci. Biofilms promote the survival of enterococci and reduce the effectiveness of drug treatment in clinical settings, hence giving enterococci an advantage. Enterococci growing in biofilms exchange traits by means of horizontal gene transfer, but currently available models make study difficult. This work goes some way to providing a non-destructive, molecular imaging-based model system for the detection of antibiotic resistance gene transfer in enterococci.

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Weisser, Maja, Selja Capaul, Marc Dangel, et al. "Additive Effect of Enterococcus faecium on Enterococcal Bloodstream Infections: A 14-Year Study in a Swiss Tertiary Hospital." Infection Control & Hospital Epidemiology 34, no. 10 (2013): 1109–12. http://dx.doi.org/10.1086/673145.

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We investigated whether an increase in enterococcal bloodstream infections (BSIs) depends on the emergence of Enterococcus faecium in an area with low vancomycin-resistant enterococci prevalence. From 1999 to 2012, a linear increase in E. faecium BSI rates (0.009 per 1,000 patient-days per year; P<.001) was noted. Enterococcus faecalis BSI rates remained stable.

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Dolka, Beata, Mariola Gołębiewska–Kosakowska, Krzysztof Krajewski, et al. "Occurrence of Enterococcus spp. in poultry in Poland based on 2014–2015 data." Medycyna Weterynaryjna 73, no. 4 (2017): 220–24. http://dx.doi.org/10.21521/mw.5680.

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Bacteria of the genus Enterococcus are mainly commensals building natural microflora in the digestive tract of birds and mammals. They belong to the potentially pathogenic microorganisms. Among poultry, infections caused by enterococci were reported in chickens, turkeys, ducks and ostriches. The aim of this study was to evaluate the occurrence of enterococci in poultry in Poland, including identification of enterococcus species composition and determination of the age of birds. The analysis was based on data obtained from 2014 – 2015 from Division of Avian Diseases at Warsaw University of Life Sciences-SGGW and four veterinary laboratories in Poland: Lab – Vet, Tarnowo Podgórne; SLW Biolab, Ostróda; Vetdiagnostica, Solec Kujawski; Vet – Lab Brudzew. Seven enterococcal species were isolated from broiler chickens (CB), commercial layers (CL), and broiler breeder flocks (BB), nine from all poultry types (chickens, turkey, ducks and geese). The most often isolated enterococci from CB were E. faecalis (57%) > E. cecorum (7%) > E. faecium (5.2%) > E. hirae (3.6%) > E. gallinarum (2.5%) > E. casseliflavus (0.7%) > E. durans (0.2%). Seven Enterococcus species were isolated from sources associated with poultry, most often E. faecalis > E. faecium > E. cecorum > E. hirae. The differences in the occurrence of particular enterococcal species were observed between CB, BB and CL. The mean age at the time of isolation of E. cecorum was approx.: 3.6 weeks in CB, 27.5 weeks in BB, 33.3 weeks in CL, 12.9 weeks in turkeys, 3.6 weeks in ducks, 39.5 weeks in geese. E. faecalis and E. faecium dominated in samples obtained from hatching eggs, dead-in-shell embryos and from samples related to poultry environment. In conclusion, this study indicates the high prevalence of bacteria of the Enterococcus genus in poultry. The present findings demonstrate the differences in Enterococcus species between poultry groups, including with regard to age. In total 10 enterococcal species (E. faecalis, E. cecorum, E. hirae, E. faecium, E. gallinarum, E. casseliflavus, E. durans, E. avium E. thailandicus, E. aquimarinus) were detected in poultry, poultry environmental samples, hatching eggs and dead-in-shell embryos. Enterococcus faecalis and E. cecorum were found in all above-mentioned sources.

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KIM, YEONG BIN, HYUN JOO SEO, KWANG WON SEO, et al. "Characteristics of High-Level Ciprofloxacin-Resistant Enterococcus faecalis and Enterococcus faecium from Retail Chicken Meat in Korea." Journal of Food Protection 81, no. 8 (2018): 1357–63. http://dx.doi.org/10.4315/0362-028x.jfp-18-046.

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ABSTRACTGenes encoding ciprofloxacin resistance in enterococci in animals may be transferred to bacteria in the animal gut and to zoonotic bacteria where they could pose a human health hazard. The objective of this study was to characterize antimicrobial resistance in high-level ciprofloxacin-resistant (HLCR) Enterococcus faecalis and Enterococcus faecium isolated from retail chicken meat. A total of 345 enterococci (335 E. faecalis and 10 E. faecium) were isolated from 200 chicken meat samples. Of these, 85 E. faecalis isolates and 1 E. faecium isolate were confirmed as HLCR enterococci. All 86 HLCR enterococci displayed gyrA-parC point mutations consisting of S83I-S80I (94.2%, 81 isolates), S83F-S80I (2.3%, 2 isolates), S83Y-S80I (2.3%, 2 isolates), and S83Y-S80F (1.2%, 1 isolate). Sixty-one (72.9%) of the 86 HLCR enterococci showed multidrug resistance to three to six classes of antimicrobial agents. Multilocus sequence typing revealed that E. faecalis had 17 different sequence types (ST) and E. faecium had 1 different ST, with ST256 observed most often (44 isolates, 51.8%). Although these results cannot exclude the possibility that pathotypes of enterococci isolated from chicken might represent transmission to or from humans, the foodborne HLCR E. faecalis indicated that the food chain is a potential route of enterococcal infection in humans.

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Tsiodras, Sotirios, Howard S. Gold, Eoin P. G. Coakley, Christine Wennersten, Robert C. Moellering, and George M. Eliopoulos. "Diversity of Domain V of 23S rRNA Gene Sequence in Different Enterococcus Species." Journal of Clinical Microbiology 38, no. 11 (2000): 3991–93. http://dx.doi.org/10.1128/jcm.38.11.3991-3993.2000.

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The highly conserved central loop of domain V of 23S RNA (nucleotides 2042 to 2628; Escherichia coli numbering) is implicated in peptidyltransferase activity and represents one of the target sites for macrolide, lincosamide, and streptogramin B antibiotics. DNA encoding domain V (590 bp) of several species ofEnterococcus was amplified by PCR. Twenty enterococcal isolates were tested, including Enterococcus faecium (six isolates), Enterococcus faecalis, Enterococcus avium, Enterococcus durans, Enterococcus gallinarum, Enterococcus casseliflavus (two isolates of each), and Enterococcus raffinosus, Enterococcus mundtii, Enterococcus malodoratus, andEnterococcus hirae (one isolate of each). For all isolates, species identification by biochemical testing was corroborated by 16S rRNA gene sequencing. The sequence of domain V of the 23S rRNA gene from E. faecium and E. faecalis differed from those of all other enterococci. The domain V sequences of E. durans and E. hirae were identical. This was also true for E. gallinarum and E. casseliflavus. E. avium differed from E. casseliflavus by 23 bases, from E. durans by 16 bases, and from E. malodoratus by 2 bases. E. avium differed fromE. raffinosus by one base. Despite the fact that domain V is considered to be highly conserved, substantial differences were identified between several enterococcal species.

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Jett, Bradley D., Rajeshwari V. Atkuri, and Michael S. Gilmore. "Enterococcus faecalis Localization in Experimental Endophthalmitis: Role of Plasmid-Encoded Aggregation Substance." Infection and Immunity 66, no. 2 (1998): 843–48. http://dx.doi.org/10.1128/iai.66.2.843-848.1998.

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ABSTRACT Enterococci have emerged as leading agents of nosocomial infection, yet relatively little is known about the pathogenesis of enterococcal disease. In previous studies, we developed an Enterococcus faecalis endophthalmitis infection model which provides unique opportunities to study the evolution of enterococcal disease by direct observation, as well as through sensitive electrophysiologic measures of organ function. The present study was designed to determine whetherE. faecalis possesses traits that permit its attachment to mammalian tissues during infection. It was also of interest to determine whether a plasmid-encoded adhesin, aggregation substance, contributes to enterococcal localization or otherwise mediates adherence to alternate sites. These studies found that, in this model, enterococci attach to membranous structures occurring within the vitreous but that this attachment or the course or severity of disease is unaffected by the aggregation substance phenotype.

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Plus de sources

Thèses sur le sujet "Enterococcus faecali"

PEREGO, FEDERICA. "DEXTRAN-SHELLED OXYGEN-LOADED NANODROPLETS PROMOTE ENTEROCOCCUS FAECALIS KILLING AND REDUCE MACROPHAGES-MEDIATED INFLAMMATION." Doctoral thesis, Università degli Studi di Milano, 2022. http://hdl.handle.net/2434/889850.

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Introduzione: Nei paesi sviluppati, le ulcere croniche rappresentano un problema sociale ed economico crescente, soprattutto negli anziani, a causa della mancanza di trattamenti efficaci e a basso costo. Nell’ambiente ipossico ed infiammatorio delle ulcere croniche i macrofagi perdono la capacità di transire dal fenotipo M1 pro-infiammatorio al fenotipo M2 antinfiammatorio. In aggiunta, i macrofagi contrastano le infezioni fagocitando ed eliminando i batteri attraverso diversi meccanismi, tra i quali la produzione di radicali dell’ossigeno e dell’azoto. Eneterococcus faecalis (E. faecalis) è un battere commensale Gram-positivo che colonizza il tratto gastrointestinale, ma anche capace di infettare le ferite e di sviluppare un’elevata resistenza agli antibiotici. Dal momento che nanogocce con guscio di destrano e cariche di ossigeno (OLNDs), in grado di rilasciare ossigeno lentamente nel tempo, si sono dimostrate in grado di contrastare gli effetti dell’ipossia su cellule endoteliali e monociti, lo scopo di questo lavoro è stato di studiare gli effetti delle OLNDs sul killing di E. faecalis mediato da macrofagi, e di valutare la modulazione dello stato infiammatorio, sia in normossia sia in ipossia. Metodi: La citotossicità delle OLNDs e OFNDs (nangocce non ossigenate) è stata valutata con saggio MTT sia su macrofagi da midollo osseo di topo (BMDM) sia su monociti umani da paziente leucemico differenziati in macrofagi con la PMA (dTHP-1). La localizzazione citoplasmatica delle OLNDs coniugate con il FITC è stata confermata con la microscopia a fluorescenza. Gli effetti delle nanogocce su E. faecalis ATCC 29212 sono stati valutati calcolando la MIC e la MBC. La produzione dei radicali liberi dell’ossigeno e dell’azoto è stata valutata usando rispettivamente il sale fluorescente H2DCFDA e il saggio di Griess. La fagocitosi e il killing di E. faecalis sono stati valutati contando il numero di macrofagi infetti su vetrini colorati con Giemsa o contando il numero di CFUs dopo il saggio di protezione dell’antibiotico e le successive 24 ore di trattamento. La produzione di citochine infiammatorie e di fattori angiogenici è stata valutata con saggio ELISA. Tutti gli esperimenti sono stati condotti in normossia e ipossia. Risultati: Le nanogocce non sono risultate tossiche fino alla concentrazione del 10% v/v, e sono state internalizzate sia dai macrofagi murini sia umani. Le nanogocce non hanno interferito con la crescita e la vitalità batterica. Le OLNDs hanno indotto i livelli di ROS e di NO nei BMDM, invece, i meccanismi di killing delle dTHP-1 non sono stati modificati. Dato l’incremento dei ROS e di NO, come atteso, il killing di E. faecalis da parte dei macrofagi murini è risultato essere migliorato dalle OLNDs e dalle OFNDs. Nessuna differenza è stata evidenziata tra nanogocce ossigenate e non ossigenate suggerendo che gli effetti erano probabilmente dovuti al guscio di destrano. Al contrario, il killing mediato dalle dTHP-1 non è stato modificato dalle nanogocce. Nella seconda parte del lavoro, l’effetto delle nanogocce sui mediatori dell’infiammazione e dell’angiogenesi è stato valutato nei sovranatanti delle dTHP-1 infette con E. faecalis. Le OLNDs e le OFNDs hanno mostrato un’attività antinfiammatoria riducendo le citochine pro-infiammatorie IL-1beta, IL-6 e TNFalfa in normossia e ipossia, di nuovo senza differenze tra i due carriers. Diversamente, come atteso, l’ipossia ha indotto il VEGF e solo le OLNDs hanno ripristinato un fenotipo simile alla normossia, confermando il rilascio di ossigeno. Conclusioni: In questo lavoro abbiamo ulteriormente confermato il potenziale delle OLNDs in qualità di tools innovativi per le ferite croniche evidenziando il loro ruolo duale nel migliorare il killing batterico e nel ridurre la risposta infiammatoria mediata dai macrofagi. Background: Chronic wounds represent nowadays an increasing social and economic burden in developed countries, especially in the elderly, due to the lack of effective and low-cost treatments. In the hypoxic and inflammatory milieu of non-healing ulcers, macrophages are unable to switch from the M1 pro-inflammatory to the M2 anti-inflammatory phenotype. Moreover, they counteract overlapping infections by phagocyting and killing bacteria with different mechanisms, among which the production of oxygen and nitrogen radicals. Enterococcus faecalis (E. faecalis) is a commensal Gram-positive bacterium, usually found in gastrointestinal tract, able to infect wounds and to develop high resistance to antibiotics. Since dextran-shelled oxygen-loaded nanodroplets (OLNDs), carriers able to release oxygen in a time-sustained manner, were proposed as tools able to counteract hypoxia in endothelial cells and monocytes, the aim of this work was to investigate their effects on macrophages-mediated killing of E. faecalis, and to evaluate their modulation of the inflammatory milieu, both in normoxia and hypoxia. Methods: Cytotoxicity of OLNDs and OFNDs (oxygen-free nanodroplets) on murine bone marrow-derived macrophages (BMDM) and human leukemic monocytes differentiated into macrophages by PMA (dTHP-1) was evaluated by MTT assay. FITC-conjugated OLNDs internalization in cytoplasm was analyzed by fluorescent microscopy. Effects of nanodroplets on E. faecalis ATCC 29212, was evaluated by MIC and MBC. The production of oxygen and nitrogen radicals were evaluated using the fluorescent dye H2DCFDA and the Griess assay, respectively. E. faecalis phagocytosis and killing were evaluated counting infected macrophages on Giemsa-stained slides or CFUs after the antibiotic protection assay and subsequent 24 hours of treatment. The production of inflammatory cytokines and angiogenic factors was evaluated by ELISA. All the experiments were performed in normoxia or hypoxia. Results: Nanodroplets were not toxic up to the concentration of 10% v/v and were internalized by both murine and human macrophages. They did not interfere with bacterial growth and viability. ROS and NO levels were induced in BMDM in presence of OLNDs, while dTHP-1 killing mechanisms were not affected by nanodroplets. Given the increase in NO and ROS in the presence of NDs, as expected, E. faecalis killing by murine macrophages was improved by OLNDs and OFNDs. No differences were observed between OLNDs and OFNDs indicating that the effects were probably due to the outer dextran shell of the NDs. On the contrary, dTHP-1-mediated killing was not affected by nanodroplets. In the second part of the work, the effects of nanodroplets on inflammatory and angiogenic mediators was evaluated in E. faecalis infected dTHP-1. OLNDs and OFNDs exerted an anti-inflammatory activity by reducing the pro-inflammatory cytokines IL-1beta, IL-6 and TNFalpha in normoxia and hypoxia. Again, no differences between OLNDs and OFNDs effects were observed. Differently VEGF, as expected, was induced by hypoxia and, only OLNDs were able to restore a normoxia-like phenotype reducing VEGF secretion and confirming the release of oxygen by OLNDs. Conclusions: We further confirmed the potential of OLNDs as innovative tools for chronic wounds by deciphering their dual role in improving bacterial killing and reducing inflammatory response by macrophages.

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Sumi, Jáuregui Ada Elizabeth. "Transferencia de plásmidos con resistencia a antibióticos en especies de Enterococcus provenientes del mar de Lima." Universidad Nacional Mayor de San Marcos. Programa Cybertesis PERÚ, 2008. http://www.cybertesis.edu.pe/sisbib/2008/sumi_ja/html/index-frames.html.

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El género Enterococcus es conocido por ser de origen fecal o intestinal, pero tiene una amplia distribución en la naturaleza y se le puede encontrar en suelos, aguas, plantas y en productos alimenticios, siendo capaz de sobrevivir en medios poco enriquecidos. Los estudios reportados sobre estos microorganismos generalmente inciden en su aspecto clínico y su resistencia a antibióticos, y algunos se ubican en un contexto ambiental evaluando métodos para su detección o enumeración para uso en aguas recreacionales. Está aumentando la importancia de este microorganismo como agente causal de infecciones adquiridas en hospitales, pero el interés de estudio en este género radica en su alta resistencia natural a múltiples antimicrobianos y a su capacidad de adquirir y transferir dicha resistencia. Se sabe que Enterococcus es un microorganismo introducido al ecosistema marino debido a la contaminación de éste ambiente con desechos orgánicos, pero son pocos los reportes sobre estudios de resistencia antimicrobiana de éste género provenientes de muestras de agua de mar, siendo necesario este tipo de investigación que nos permita conocer la importancia de estos microorganismos en estos ambientes The genus Enterococcus is recognized as being of fecal origin but have a wide distribution in nature, they can be found in soil, water, plants and food products, being able to survive in low-enriched media. Studies on these microorganisms usually affect their appearance and clinical resistance to antibiotics, and there are some who are placed in an environmental context, evaluating methods of detection or enumeration in waters for recreational use. It is increasing the importance of this microorganism as a causative agent of infections acquired in hospitals, but the interest in this kind of study lies in its high natural resistance to multiple antimicrobials and their ability to acquire and transfer the resistance. Despite that Enterococcus is a microorganism introduced to the marine ecosystem by contamination with organic wastes, there are few reports on studies of antimicrobial resistance of the Enterococcus genus water samples from the sea, being necessary to this type of research that allows us to know the importance of these microorganisms in these environments

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Sumi, Jáuregui Ada Lizbeth. "Transferencia de plásmidos con resistencia a antibióticos en especies de Enterococcus provenientes del mar de Lima." Bachelor's thesis, Universidad Nacional Mayor de San Marcos, 2008. https://hdl.handle.net/20.500.12672/907.

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El género Enterococcus es conocido por ser de origen fecal o intestinal, pero tiene una amplia distribución en la naturaleza y se le puede encontrar en suelos, aguas, plantas y en productos alimenticios, siendo capaz de sobrevivir en medios poco enriquecidos. Los estudios reportados sobre estos microorganismos generalmente inciden en su aspecto clínico y su resistencia a antibióticos, y algunos se ubican en un contexto ambiental evaluando métodos para su detección o enumeración para uso en aguas recreacionales. Está aumentando la importancia de este microorganismo como agente causal de infecciones adquiridas en hospitales, pero el interés de estudio en este género radica en su alta resistencia natural a múltiples antimicrobianos y a su capacidad de adquirir y transferir dicha resistencia. Se sabe que Enterococcus es un microorganismo introducido al ecosistema marino debido a la contaminación de éste ambiente con desechos orgánicos, pero son pocos los reportes sobre estudios de resistencia antimicrobiana de éste género provenientes de muestras de agua de mar, siendo necesario este tipo de investigación que nos permita conocer la importancia de estos microorganismos en estos ambientes. The genus Enterococcus is recognized as being of fecal origin but have a wide distribution in nature, they can be found in soil, water, plants and food products, being able to survive in low-enriched media. Studies on these microorganisms usually affect their appearance and clinical resistance to antibiotics, and there are some who are placed in an environmental context, evaluating methods of detection or enumeration in waters for recreational use. It is increasing the importance of this microorganism as a causative agent of infections acquired in hospitals, but the interest in this kind of study lies in its high natural resistance to multiple antimicrobials and their ability to acquire and transfer the resistance. Despite that Enterococcus is a microorganism introduced to the marine ecosystem by contamination with organic wastes, there are few reports on studies of antimicrobial resistance of the Enterococcus genus water samples from the sea, being necessary to this type of research that allows us to know the importance of these microorganisms in these environments. Tesis

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Roberts, Gretta. "Glycoprotein utilisation by enterococcus faecalis." Thesis, King's College London (University of London), 2004. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.412446.

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Barros, Mariana Vilhena. "Infeções nosocomiais por enterococcus faecalis." Master's thesis, [s.n.], 2014. http://hdl.handle.net/10284/4512.

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Projeto de Pós-Graduação/Dissertação apresentado à Universidade Fernando Pessoa como parte dos requisitos para obtenção do grau de Mestre em Ciências Farmacêuticas As infeções nosocomiais são consideradas um problema mundial de saúde pública. A sua disseminação tem contribuído para aumento das taxas de mortalidade e morbilidade, a maioria das vezes devido às limitadas ou mesmo inexistentes opções terapêuticas. Enterococcus faecalis é uma bactéria Gram-positiva, anaeróbia facultativa, presente na flora comensal do trato gastrintestinal de humanos e animais. Apesar da sua suposta inocuidade, nas últimas décadas E.faecalis tem-se revelado um patogénio oportunista, representando a segunda e a terceira maior causa de infeções hospitalares a nível mundial. Esta bactéria é apontada como uma das principais causas de endocardites, bacteremias, infeções do trato urinário, intra-abdominais e de feridas contraídas em hospitais. As suas caraterísticas fisiológicas permitem-lhe sobreviver a altas temperaturas, a elevados valores de pH e concentrações salinas. Esta bactéria resiste também em ambientes hostis, em situações de subnutrição, de stress oxidativo e às técnicas tradicionais de limpeza. Apresenta inúmeros fatores de virulência nomeadamente proteínas de superfície, enzimas hidrolíticas e capacidade de formação de biofilmes, o que auxilia esta bactéria a invadir, colonizar e infetar tecidos hospedeiros. Enterococcus faecalis exibe uma resistência intrínseca a algumas classes de antibióticos como β-lactâmicos, lincosamidas, trimetropim-sulfametoxazol, fluoroquinolonas e baixas concentrações de aminoglícosídeos. Devido à sua capacidade mutagénica e adaptativa, este microrganismo desenvolve e adquire novas resistências, através de mutações cromossomais ou por transferência de genes. Como é o caso do gene vanA e vanB, que lhe conferem resistência à vancomicina (VRE). E.faecalis tem-se revelado uma constante ameaça de vida em todo o mundo, por isso é urgente controlar a disseminação desta bactéria. É importante desenvolver novos métodos terapêuticos, tendo em conta a ineficácia dos atuais e aplicar estratégias, a nível hospitalar, que diminuam ao máximo a transmissão do microrganismo. The nosocomial infections are nowadays a worldwide issue in terms of health. Its dissemination has been the cause of the rising of mortality and disease rates, most of the time due to limited or even nonexistent therapeutic options. Enterococcus faecalis is a Gram-positive anaerobic facultative bacterium witch is found in the flora of the gastrointestinal tract of humans and animals. Despite its alleged harmlessness, lately E.faecalis has been proved to be an opportunistic pathogen, representing the second and the third leading cause of hospital-acquired infections worldwide. This bacterium is being pointed as a mainly cause of endocarditis, fast progressing bacteremia may present, urinary tract infections, intra-abdominal and wounds contracted in hospitals. Its physiological characteristics allows this bacterium to survive at high temperatures, high PH values and highly concentration salts. It can also resist in hostiles environments such as malnutrition, oxidative stress and traditional cleaning technics. It feature numerous virulence factors including surface proteins, hydrolytic enzymes and ability of biofilm formation, which helps the bacterium to invade, colonize and infect the host tissue. Enterococcus faecalis displays an intrinsic resistance to some classes of antibiotics such as β-lactam, lincosamides, trimetropim-sulfamethoxazole, fluoroquinolones and low concentration of aminoglycosides. Due to its mutagenic capacity and adaptive skills, this micro-organism develops and acquires new resistances, through chromosome mutations or by genetic transferences e.g. gene vanA e vanB, witch confers resistance to Vancomycin (VRE). E. faecalis has proved a constant threat of life throughout the world, so there is an urgent need to control the spread of this bacteria by developing new therapeutic methods, having regard to the ineffectiveness of current ones and looking for new strategies inside hospitals that will reduce as much as possible the transmission of the micro-organism.

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Böttcher, Daiana Elisabeth. "Avaliação do efeito da presença do biofilme de Enterococcus faecalis no canal radicular sobre a manutenção da substantividade da clorexidina a 2% : estudo in vitro." reponame:Biblioteca Digital de Teses e Dissertações da UFRGS, 2014. http://hdl.handle.net/10183/110768.

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Introdução: O objetivo do presente estudo foi correlacionar o efeito antimicrobiano residual e a substantividade da solução de clorexidina a 2%, em dentina humana de dentes extraídos e contaminada com E. faecalis, por 48 horas, 7 e 30 dias. Metodologia: Cento e vinte e três dentes humanos extraídos foram utilizados para esse estudo. As amostras foram divididas em quatro grupos conforme a solução irrigadora utilizada (CHX 2% ou soro fisiológico) e na presença ou ausência do biofilme de Enterococcus faecalis. As amostras foram mantidas em contato com a respectiva solução durante 5 minutos. Cada grupo foi distribuído aleatoriamente em 3 subgrupos de acordo com o período de avaliação (n=10). A quantidade de CHX presente foi avaliada através de cromatografia líquida de alta eficiência (HPLC) e a viabilidade bacteriana foi analisada através de microscópio confocal a laser (CLSM). A análise estatistica foi feita através dos testes de Kruskal-Wallis e Mann-Whitney U (P<.05) e teste de correlação de Spearman (P<.01). Resultados: Houve uma correlação negativa entre o percentual de células viáveis e a quantidade de CHX remanescente (P = .000). A CHX reduziu significativamente o percentual de células viáveis em relação ao soro após 48 horas (P = .007). A diferença foi mantida no período de 7 dias (P = .001). Após 30 dias, o grupo CHX apresentou um aumento da viabilidade bacteriana tornando-se semelhante ao soro (P = .623). Simultaneamente, a quantidade de CHX reduziu significativamente após 30 dias (P = .000). Conclusão: Os resultados do presente estudo indicam que a CHX a 2% foi detectada nos periodos de 48 horas e 7 dias, mantendo reduzido o percentual de células viáveis. A presença de microrganismos na dentina humana não alterou a quantidade de CHX residual. Introduction: The aim of this study was to correlate the bacterial viability and the presence of 2% chlorhexidine (CHX) on dentin by means of confocal laser scanning microscope (CLSM) and high-performance liquid chromatography (HPLC) for 48 hours, 7 and 30 days. Methods: One hundred twenty three extracted human teeth were used. Samples were divided into 4 groups according to the solution (CHX or saline) and the presence of Enterococus faecalis biofilm. Samples were kept in contact with 5mL of the solution for 5 minutes. Each group was divided into 3 subgroups according to the evaluation period (n = 10). Statistical analysis was performed by using the Kruskal- Wallis, Mann-Whitney U tests (P < .05) and Spearman’s Rank Correlation Coefficient (P < .01). Results: There was a negative correlation between the percentage of live cells and the amount of remaining CHX (P = .000). CHX significantly reduced the percentage of viable cells compared to saline after 48 hours (P = .007). Differences were maintained in the 7-day evaluation (P = .001). After 30 days, CHX group presented an increase of viable cells, thereby becoming similar to saline (P = .623). Simultaneously, remaining CHX significantly reduced in the 30-day specimens (P = .000). Conclusion: The results of this study indicate that 2% CHX solution was detected for 48 hours and 7 days, keeping a low percentage of viable cells. The presence of microorganisms on human dentin did not affect 2% chlorhexidine maintenance.

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Tse, Chee-choong Micheal, and 謝志聰. "Effect of ultrasonic agitation on enterococcus faecalis biofilm." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2010. http://hub.hku.hk/bib/B45165993.

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Abadía, Patiño Lorena. "Caractérisation de l'opéron vanE chez Enterococcus faecalis BM4405." Paris 7, 2003. http://www.theses.fr/2003PA077001.

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Diederich, Ann-Kristin [Verfasser], and Johannes [Akademischer Betreuer] Hübner. "Membrane lipids of Enterococcus faecalis as microbial pathogens." Freiburg : Universität, 2016. http://d-nb.info/1119452708/34.

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Neto, Manoel Matos. "Avaliação in vitro da eficácia de técnicas endodônticas de preparo mecânico na redução de Enterococcus faecalis." Universidade de Taubaté, 2007. http://www.bdtd.unitau.br/tedesimplificado/tde_busca/arquivo.php?codArquivo=483.

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O preparo mecânico tem por objetivo a modelagem dos canais radiculares e redução de microrganismos nele presentes. Para sua execução foram utilizados instrumentos endodônticos confeccionados em aço inoxidável ou ligas de níquel-titânio. Esse estudo tem como objetivo avaliar a eficácia de três sistemas de preparo mecânico na redução da contagem de Enterococcus faecalis no interior de canais radiculares. Para isso foram utilizados 24 dentes humanos unirradiculares, divididos em três grupos: G1 - instrumentação com o sistema ProTaper rotatório, G2 - com sistema ProTaper manual e G3 - com limas tipo Kerr manuais. Os canais radiculares foram contaminados com Enterococcus faecalis, e após formação de biofilme, submetidas ao preparo mecânico. As unidades formadoras de colônia foram contadas antes e depois da instrumentação e a porcentagem de redução calculada. Os resultados demonstraram que todos os sistemas reduziram significantemente a quantidade de microrganismos dentro do canal radicular. Contudo, quando se comparou a redução promovida pelos sistemas entre si não houve diferença significativa. O estudo confirmou a eficácia do preparo mecânico, entretanto nenhum dos sistemas avaliados foi superior ao outro na redução do número de Enterococcus faecalis no interior dos canais radiculares. The objects of mechanical preparation are to model root canals and reduce the number of microorganisms present in them. For this purpose, endodontic instruments made of stainless steel or nickel-titanium alloys are used. The aim of this study was to assess the efficacy of three systems of mechanical preparation for reducing the counting of Enterococcus faecalis inside root canals. To do this, 24 single rooted human teeth were used, divided into three groups: G1 instrumentation with the ProTaper rotary system, G2 with the manual ProTaper system and G3 manual Kerr type files. The root canals were contaminated with Enterococcus faecalis, and after biofilm had formed, they were submitted to mechanical preparation. The colony forming units were counted before and after instrumentation and the percentage of reduction was calculated. The results demonstrated that all the systems significantly reduced the number of microorganisms inside the root canal. However, when the reduction promoted by the systems was compared among them, there was no significant difference. The study confirmed the efficacy of mechanical preparation, however, none of the assessed systems was superior to the other for reducing the number of microorganisms inside root canals.

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Livres sur le sujet "Enterococcus faecali"

Cattelan, Jessy. Immunologische Studie zum Oberflächenprotein TraM, ein Typ IV Sekretionsprotein in Enterococcus faecalis / Enterococcus faecium. s.n.], 2014.

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Berthold, Martin. Die Pathogenität biofilmassoziierter Virulenzfaktoren von Enterococcus faecalis im Endokarditismodell. s.n.], 2014.

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Shorrock, Patricia Joan. Surface properties of enterococcus faecalis in relation to infective endocarditis. Aston University. Department of Pharmaceutical Sciences, 1990.

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Bao, Yinyin. Role of mprF1 and mprF2 in the pathogenicity of Enterococcus faecalis. Universität, 2012.

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Peters, Tansy M. Studies on the activation of azo-dyes into direct-acting genotoxic agents by enterococcus faecalis. University of East London, 1995.

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Mack, Henry L. Enterococcus Faecalis: Molecular Characteristics, Role in Nosocomial Infections and Antibacterial Effects. Nova Science Publishers, Incorporated, 2014.

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Gina, Pugliese, and Weinstein Robert A. 1946-, eds. Issues & controversies in prevention and control of VRE, vancomycin-resistant enterococci. Etna Communications, 1998.

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Bambawale, Amrut, Sumita Bhagwat, and Leena Padhye. Aktywność przeciwdrobnoustrojowa Propolis, HEBP, chlorheksydyny, EDTA: Przeciwko Enterococcus Faecalis i Candida Albicans ; badanie Invitro. Wydawnictwo Nasza Wiedza, 2020.

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Shepard, Brett David. Differential expression of virulence-related genes by Enterococcus faecalis in response to biological cues. [s.n.], 2002.

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Herrington, William G., Aron Chakera, and Christopher A. O’Callaghan. Urinary tract infection. Edited by Patrick Davey and David Sprigings. Oxford University Press, 2018. http://dx.doi.org/10.1093/med/9780199568741.003.0158.

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A urinary tract infection (UTI) is defined as present when urine culture results in the growth of a single organism at greater than 105 colony-forming units/ml of urine. Bowel flora are the commonest cause of UTIs. Escherichia coli accounts for 80% of infections. Klebsiella spp., Proteus mirabilis, Enterococcus faecalis, and Staphylococcal saprophyticus account for most of the remaining 20%. Staphylcoccus aureus culture is usually catheter related or secondary to haematogenous spread. This chapter looks at the symptoms of a UTI, as well as its demographics, complications, diagnosis (including investigations), and treatment. It also discusses prognosis.

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Chapitres de livres sur le sujet "Enterococcus faecali"

Murray, Barbara E., Liangxia Jiang, Jianguo Xiao, et al. "Enterococcus faecalis." In Bacterial Genomes. Springer US, 1998. http://dx.doi.org/10.1007/978-1-4615-6369-3_59.

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Manson, Janet M., and Michael S. Gilmore. "Pathogenomics of Enterococcus faecalis." In Pathogenomics. Wiley-VCH Verlag GmbH & Co. KGaA, 2006. http://dx.doi.org/10.1002/352760801x.ch7.

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McShan, W. Michael, and Nathan Shankar. "The Genome of Enterococcus faecalis V583: a Tool for Discovery." In The Enterococci. ASM Press, 2014. http://dx.doi.org/10.1128/9781555817923.ch11.

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Wirth, Reinhard. "The sex pheromone system of Enterococcus faecalis." In EJB Reviews 1994. Springer Berlin Heidelberg, 1994. http://dx.doi.org/10.1007/978-3-642-79502-2_9.

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Fujimoto, S., M. Bastos, K. Tanimoto, F. An, K. Wu, and D. B. Clewell. "The pAD1 Sex Pheromone Response in Enterococcus faecalis." In Streptococci and the Host. Springer US, 1997. http://dx.doi.org/10.1007/978-1-4899-1825-3_244.

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Clermont, Dominique, Gilda de Cespédès, Françoise Delbos, and Théa Horaud. "Genetic Analysis of Enterococcus faecalis Chromosome Carrying Mobile Elements." In Streptococci and the Host. Springer US, 1997. http://dx.doi.org/10.1007/978-1-4899-1825-3_241.

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Shankar, Viswanathan, and Michael S. Gilmore. "Characterization of the Enterococcus faecalis Alpha C Protein Homolog." In Streptococci and the Host. Springer US, 1997. http://dx.doi.org/10.1007/978-1-4899-1825-3_246.

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Nakayama, Jiro, Yuuichiro Takanami, and Akinori Suzuki. "Analysis of Pheromone Binding and Pheromone Reception by Enterococcus faecalis." In Streptococci and the Host. Springer US, 1997. http://dx.doi.org/10.1007/978-1-4899-1825-3_243.

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Pasquarella, Cesira, Donald Morrison, Angelo Savino, and Barry D. Cookson. "Dynamics of Enterococcus faecalis Colonization of Bone Marrow Transplant Patients." In Streptococci and the Host. Springer US, 1997. http://dx.doi.org/10.1007/978-1-4899-1825-3_68.

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Clewell, Don B. "Sex Pheromones and the Plasmid-Encoded Mating Response in Enterococcus faecalis." In Bacterial Conjugation. Springer US, 1993. http://dx.doi.org/10.1007/978-1-4757-9357-4_14.

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Actes de conférences sur le sujet "Enterococcus faecali"

Santos, Pâmela Gomes, Rosane Nassar Meireles Guerra, Josivan Regis Farias, Simone Batista Muniz, and Danielle Cristine Gomes Franco. "AÇÃO ANTIMICROBIANA DAS FLORES DE ANACARDIUM OCCIDENTALE E DO ÁCIDO ELÁGICO PRESENTE NO EXTRATO." In I Congresso Brasileiro de Imunologia On-line. Revista Multidisciplinar em Saúde, 2021. http://dx.doi.org/10.51161/rems/965.

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Introdução: As infecções bacterianas têm aumentado significativamente nas últimas décadas, sobretudo aquelas ocasionadas por microrganismos multirresistentes. Assim, o uso de produtos naturais com finalidades terapêuticas surge com alvo de bioprospecção na busca de novos compostos com ação antimicrobiana. Além disso, o uso de insetos, como o Tenebrio molitor como modelo experimental para avaliação in vivo tem sido muito frequente, pois exige menos material em relação aos testes com animais vertebrados. Objetivo: O presente trabalho investigou o efeito citotóxico e ação antimicrobiana do extrato hidroalcoólico das flores de Anacardium occidentale (EHAo) e do ácido elágico. Material e Métodos: Avaliamos a citoxicidade de ácido elágico e do EHAo nas concentrações (1; 5 e 50mg/kg) em Tenebrio molitor. A ação antimicrobiana para Enterococcus faecalis, Staphylococcus aureus, Streptococcus mutans, Escherichia coli, Pseudomonas aeruginosa e Klebsiella pneumoniae e a toxicidade do ácido elágico, foi avaliada por microdiluição, segundo a norma M7-A6 do manual da Clinical and Laboratory Standards Institute – CLSI. Foi determinada a Concentração Bactericida Mínima (CBM) e concentração inibitória mínima (CIM), em culturas de 24 horas, incubadas à 37ºC. Resultados: No ensaio de citotoxidade aguda se verificou que nenhuma das concentrações usados foram tóxicas, pois não ocorreram óbitos e nem nenhuma anormalia morfológica nas larvas de Tenebrio molitor. Os testes de concentração inibitória mínima (CIM) de concentração bactericida mínima (CBM) mostraram que o EHAo apresentou ação bactericida para Enterococcus faecalis em todas as concentrações testadas. Para Staphylococcus aureus os resultados mostraram ação bactericida para as maiores concentrações e bacteriostática para a menor diluição. O ácido elágico teve ação bactericida apenas para Enterococcus faecalis. Para as bactérias Streptococcus mutans, Escherichia coli, Pseudomonas aeruginosa e Klebsiella pneumoniae as concentrações de EHAo e ácido elágico testadas não foram inibitórias. Conclusões: Os resultados mostraram baixa toxicidade tanto para o EHAo como para o ácido elágico e ainda, que o extrato apresentou melhor efeito antimicrobiano do que o ácido elágico, para Enterococcus faecalis e Staphylococcus aureus.

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Keyhani, Jacqueline, and Ezzatollah Keyhani. "Increased resistance to detergent in Enterococcus faecalis." In Proceedings of the International Conference on Antimicrobial Research (ICAR2010). WORLD SCIENTIFIC, 2011. http://dx.doi.org/10.1142/9789814354868_0010.

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Du, Juan, Hofung Cheng, Ken T. M. Wong, and Stanley C. K. Lau. "A microcosm study on the die-off response of the indicator bacteria, Enterococcus faecium and Enterococcus faecalis." In MICROBES IN APPLIED RESEARCH - Current Advances and Challenges. WORLD SCIENTIFIC, 2012. http://dx.doi.org/10.1142/9789814405041_0001.

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Tverezovska, Olesia, Viktoriia Holubnycha, Rafal Banasiuk, Yevheniia Husak, Savchenko Anton, and Viktoriia Korniienko. "The Effect of Silver Nanoparticles Against Formation of Enterococcus Faecalis Biofilms." In 2022 IEEE 12th International Conference Nanomaterials: Applications & Properties (NAP). IEEE, 2022. http://dx.doi.org/10.1109/nap55339.2022.9934155.

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Ahmed, Mohamed O., Peter D. Clegg, Nicola J. Williams, Keith E. Baptiste, and Malcolm Bennett. "Vancomycin Resistant Enterococci (VRE) in equine-faecal samples." In Proceedings of the International Conference on Antimicrobial Research (ICAR2010). WORLD SCIENTIFIC, 2011. http://dx.doi.org/10.1142/9789814354868_0070.

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LUIS BAPTISTA DE GUSMÃO, PEDRO, THAIS DA SILVA FEITOSA, Brenda Paula Figueiredo de Almeida Gomes, Eloá Cristina Bícego-Pereira, and MAICON RICARDO ZIEBERG PASSINI. "Isolamento e identificação de Enterococcus faecalis de dentes submetidos à reintervenção endodôntica." In XXIV Congresso de Iniciação Científica da UNICAMP - 2016. Galoa, 2016. http://dx.doi.org/10.19146/pibic-2016-51716.

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Fernandes, Meg da Silva, Luciana Maria Ramires Esper, Dirce Yorika Kabuki, and Arnaldo Yoshiteru Kuaye. "Produção de Moléculas Sinalizadoras de Quorum Sensing por Cepas de Enterococcus faecium e Enterococcus faecalis Isoladas do Processamento de Ricota." In V Simpósio de Bioquímica e Biotecnologia. Editora Edgard Blücher, 2015. http://dx.doi.org/10.5151/biochem-vsimbbtec-21938.

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Ke Sun, Jue Zhang, Jing Fang, Jing Wang, Jie Pan, and Weidong Zhu. "Cold plasma therapy for enterococcus faecalis biofilm infected tooth root canal in vitro." In 2012 IEEE 39th International Conference on Plasma Sciences (ICOPS). IEEE, 2012. http://dx.doi.org/10.1109/plasma.2012.6383868.

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Paula Figueiredo De Almeida Gomes, Brenda, Maikon Tadeu Ferrari Martinucho, ElaÍse Gabriele Martins, and Beatriz Leonardo Prudenciano. "Avaliação de substâncias químicas auxiliares, utilizadas em endodontia, na redução de Enterococcus faecalis." In XXIII Congresso de Iniciação Científica da Unicamp. Galoá, 2015. http://dx.doi.org/10.19146/pibic-2015-38003.

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Pinto, Vanessa Arantes, Gabriela Santos Alencar, Adriano Favero, Hugo Franciscon, and Fagner Luiz Da Costa Freitas. "POTENCIAL MICROBIOLÓGICO DO SYZYGIUM AROMATICUM (L). SOBRE MICRORGANISMOS DE IMPORTÂNCIA NA MEDICINA VETERINÁRIA." In I Congresso On-line Nacional de Clínica Veterinária de Pequenos Animais. Revista Multidisciplinar em Saúde, 2021. http://dx.doi.org/10.51161/rems/1920.

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Introdução: O fenômeno de resistência a antimicrobianos está se mostrando um grande problema na saúde, ameaçando tanto a população humana como animal. Devido a isso, a descoberta de novos compostos com capacidade antimicrobiana torna-se imprescindível, e os fitoterápicos têm se mostrado uma alternativa promissora na terapia complementar. O óleo essencial (OE) de Syzygium aromaticum (Cravo da Índia), apresenta potencial capacidade antibacteriana e antifúngica devido às altas concentrações de Eugenol. Objetivos: Avaliar o potencial antimicrobiano do óleo essencial de Syzygium aromaticum (L). contra microrganismos de importância na Medicina veterinária. Materiais e métodos: Foram testadas as bactérias gram positivas: S. aureus LB 25923, S. aureus B24, S. aureus NP 38, Streptococcus uberis, S. agalactiae, Enterococcus faecalis e S. epidermidis; as gram negativas: Pseudomonas aeruginosa ATCC 27853 e E. coli ATCC 25922; e os fungos: C. tropicalis e C. gattii 179. Utilizou-se a técnica de disco difusão (Kirby-bauer) aplicando 10µL de OE, e posteriormente, para os patógenos que apresentaram o halo inibitório, determinou-se, aplicando 15µL de OE, a concentração inibitória mínima (CIM), concentração bactericida mínima (CBM) e concentração fungicida mínima (CFM) através da microdiluição seriada utilizando leitor de ELISA e corante resazurina. Resultados: Todos os microrganismos apresentaram halo inibitório. Com relação aos valores de CIM e CBM para as bactérias, obteve-se, respectivamente: S. aureus LB25923 (18,75% e 18,75%), S. aureus B 24 (4,94% e 4,94%) S. aureus NP 38 (3,64% e 3,64%) Streptococcus uberis (2,60% e 3,125%) S. agalactiae (5,20 %, 5,20%), Enterococcus faecalis (5,20% e 6,25%), S. epidermidis (3,38% e 4,42%), Pseudomonas aeruginosa ATCC 27853 (2,09% e 2,09%), E. coli ATCC 25922 (10,41% e 10,41%). Os resultados de CIM e CFM obtidos para os fungos foram respectivamente: C. tropicalis (1,82% e 1,82%) e C. gattii 179 (5,27% e 5,27%). Conclusão: O óleo essencial de Syzygium aromaticum possui considerável potencial antimicrobiano “in vitro”, e dessa forma, pode ser considerado uma possível alternativa para as terapias sintéticas de tratamento das doenças infecciosas bacterianas e fúngicas encontradas na rotina clínica.

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Rapports d'organisations sur le sujet "Enterococcus faecali"

A. Komnos, Georgios, Antonios Papadopoulos, Efstratios Athanaselis, Theofilos Karachalios, and Sokratis E. Varitimidis. Migrating Periprosthetic Infection from a Total Hip Replacement to a Contralateral Non-Operated Osteoarthritic Knee Joint. Science Repository, 2023. http://dx.doi.org/10.31487/j.ijscr.2022.03.02.

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Introduction: There is a paucity of published data on whether a treated infected arthroplasty is a risk factor for infection in another, non-operated joint. Contamination of a primary, arthritic, non-operated joint from an infected arthroplasty is a relatively rare entity. Case: We report a case of migration of a pathogen (Enterococcus faecalis) from an infected prosthetic joint (hip) to the contralateral native joint (knee). Identification of the pathogen was made with PCR, by obtaining cultures during the implantation of the primary knee prosthesis. Conclusion: Contamination of a primary, arthritic, non-operated joint from an infected arthroplasty has not been widely reported. Management of such cases is extremely challenging and without clear and established guidelines. Our experience shows that tissue samples should be taken intraoperatively and sent for cultures, so as to exclude contamination in those cases.

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Bezerra, Alexandre Sacchetti, Flavia Altheman Loureiro, Carla Maria Pasquareli Vazquez, Afonso Cesar Polimanti, and Rafi Felicio Bauab Dauar. Empiric Treatment of Foot Infection in Patients with Severe Diabetes. Science Repository, 2021. http://dx.doi.org/10.31487/j.jicoa.2021.04.04.

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Background: Despite being treated with antibiotics of broad spectrum recommended by International Consensus, severe diabetic patients with lower limb infection do not present a positive clinical evolution during empirical treatment. This study’s bacterial profile was analysed and compared with other worldwide hospital centers. Objective: To confirm the need of an individualized empirical treatment for severe diabetic patients with foot infection. Methods: Retrospective analysis of cultures and antibiograms of severe diabetic patients admitted by foot infection. Results: The results were consistent with the socioeconomic realities of developing countries. Gram-negative bacteria (52,11%) were present in most bone cultures. Results presented a high incidence of Enterococcus faecalis in both gram-positive (21,2%) and polymicrobial (34,7%) samples. Bacterial resistance with the use of ordinary antibiotics in the statistical analysis was high. Conclusion: The community infections should undergo broad spectrum empirical therapy combining amikacin (80,43%) or meropenem (72,00%) with gram-negative and vancomycin (100%) or teicoplanin (90,00%) or linezolid (74,19%) with gram-positive.

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