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Articles de revues sur le sujet « Enterococcus faecali »

Auteur : Grafiati

Publié le 9 mars 2023

Mis à jour le 19 juillet 2025

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1

Christine, Bella Fernanda, and Florenly. "Mixture of Garlic (Allium sativum) Extract 50% and Red Dragon Fruit (Hylocereus polyrhizus) peel 50% Effectiveness Against Enterococcus faecalis Bacteria." Jurnal Penelitian Pendidikan IPA 11, no. 1 (2025): 694–700. https://doi.org/10.29303/jppipa.v11i1.9905.

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Enterococcus faecalis may infiltrate dentinal tubules in the cementum and dentin of the root, causing endodontic failure. A key stage in root canal therapy is irrigation. NaOCl is an efficient irrigation at higher concentrations, although its toxicity causes periapical tissue injury. Garlic and red dragon fruit peel suppress Enterococcus faecalis. To test the antibacterial activity of garlic (Allium sativum) extract and red dragon fruit (Hylocereus polyrhizus) peel extract at 50% against Enterococcus faecalis bacterium. A post-test only control-group design was used in this in vitro study. A 5

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Maheux, Andrée F., Sébastien Bouchard, Ève Bérubé, and Michel G. Bergeron. "Rapid molecular identification of fecal origin-colonies growing on Enterococcus spp.-specific culture methods." Journal of Water and Health 15, no. 2 (2016): 239–50. http://dx.doi.org/10.2166/wh.2016.199.

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The mEI, Chromocult® enterococci, and m-Enterococcus culture-based methods used to assess water quality by the detection of Enterococcus spp. were first compared in terms of sensitivity using (1) 41 different type strains of Enterococcus spp. and (2) environmental colonies identified by 16S rRNA sequencing. Then, two specific-rtPCR assays targeting Enterococcus spp. and Enterococcus faecalis/faecium were tested for their ability to confirm the identity of putative enterococcal colonies. The mEI, Chromocult® enterococci, and m-Enterococcus methods detected β-glucosidase activity for 28 (68.3%),

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Kumurya, A. S., and B. Ega. "An Overview on Vancomycin Resistant Enterococcus faecalis." UMYU Journal of Microbiology Research (UJMR) 6, no. 1 (2021): 160–67. http://dx.doi.org/10.47430/ujmr.2161.033.

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There are over 15 species of the Enterococcus genus, 80-90% of clinical isolates as E. faecalis. The aim of this work is to review the current information on Vancomycin resistant Enterococcus fecalis. The study reviewed using electronic documents and hard copies from public libraries of relevant literatures relating to biology, epidemiology, drug resistance mechanism, treatment, and control of Enterococcus faecalis. The review revealed that Enterocuccus faecalis formerly known as Streptococcus faecalis is a Gram-positive commensal bacterium that inhabits the gastrointestinal tracts of healthy

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Cox, Christopher R., and Michael S. Gilmore. "Native Microbial Colonization of Drosophila melanogaster and Its Use as a Model of Enterococcus faecalis Pathogenesis." Infection and Immunity 75, no. 4 (2007): 1565–76. http://dx.doi.org/10.1128/iai.01496-06.

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ABSTRACT Enterococci are commensal organisms of the gastrointestinal (GI) tracts of a broad range of mammalian and insect hosts, but they are also leading causes of nosocomial infection. Little is known about the ecological role of enterococci in the GI tract consortia. To develop a tractable model for studying the roles of these organisms as commensals and pathogens, we characterized the Drosophila melanogaster microflora and examined the occurrence of enterococci in the gastrointestinal consortium of Drosophila. In a survey of laboratory-reared Drosophila and wild-captured flies, we found th

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Lauková, Andrea, Viola Strompfová, Jana Ščerbová, and Monika Pogány Simonová. "Virulence Factor Genes Incidence among Enterococci from Sewage Sludge in Eastern Slovakia following Safety Aspect." BioMed Research International 2019 (October 7, 2019): 1–5. http://dx.doi.org/10.1155/2019/2735895.

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The sewage sludges represent a potential health hazard because of the quantity of different microbiota detected in sewages. Among microbiota detected in sewages, also belong representatives of the phylum Firmicutes. In the past, environmental enterococci in addition to coliforms were widely used as indicators of faecal contamination. Regarding the enterococcal strains as potential pathogenic bacteria, their pathogenicity is mainly caused by production of virulence factors. Therefore, the aim of the study was to analyse incidence of virulence factors in enterococci from cows' dung water. Specie

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Conwell, Michael, James S. G. Dooley, and Patrick J. Naughton. "A Novel Biofilm Model System to Visualise Conjugal Transfer of Vancomycin Resistance by Environmental Enterococci." Microorganisms 9, no. 4 (2021): 789. http://dx.doi.org/10.3390/microorganisms9040789.

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Enterococci and biofilm-associated infections are a growing problem worldwide, given the rise in antibiotic resistance in environmental and clinical settings. The increasing incidence of antibiotic resistance and its propagation potential within enterococcal biofilm is a concern. This requires a deeper understanding of how enterococcal biofilm develops, and how antibiotic resistance transfer takes place in these biofilms. Enterococcal biofilm assays, incorporating the study of antibiotic resistance transfer, require a system which can accommodate non-destructive, real-time experimentation. We

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Weisser, Maja, Selja Capaul, Marc Dangel, et al. "Additive Effect of Enterococcus faecium on Enterococcal Bloodstream Infections: A 14-Year Study in a Swiss Tertiary Hospital." Infection Control & Hospital Epidemiology 34, no. 10 (2013): 1109–12. http://dx.doi.org/10.1086/673145.

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We investigated whether an increase in enterococcal bloodstream infections (BSIs) depends on the emergence of Enterococcus faecium in an area with low vancomycin-resistant enterococci prevalence. From 1999 to 2012, a linear increase in E. faecium BSI rates (0.009 per 1,000 patient-days per year; P<.001) was noted. Enterococcus faecalis BSI rates remained stable.

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Mustafa, Eman, and Amera Al-Rawi. "Molecular investigation of enterococcal surface protein (esp) gene of Enterococcus faecalis isolated from endodontic patients." Medicinski casopis 57, no. 4 (2023): 141–46. http://dx.doi.org/10.5937/mckg57-46998.

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Objective. Enterococci are generally considered transient components of oral bacteria that may be a reason for several oral and systemic infections, particularly those related to dental root canal infections. The current study aims to examine the occurrence of Enterococcus surface protein, esp in Enterococcus faecalis, which is isolated from infected root canals. Methods. Forty samples were collected from endodontic patients who attended the Conservative Treatment Department in the College of Dentistry/Mosul University/Dental Teaching Hospital. Materials and Methods: All samples were tradition

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Dolka, Beata, Mariola Gołębiewska–Kosakowska, Krzysztof Krajewski, et al. "Occurrence of Enterococcus spp. in poultry in Poland based on 2014–2015 data." Medycyna Weterynaryjna 73, no. 4 (2017): 220–24. http://dx.doi.org/10.21521/mw.5680.

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Bacteria of the genus Enterococcus are mainly commensals building natural microflora in the digestive tract of birds and mammals. They belong to the potentially pathogenic microorganisms. Among poultry, infections caused by enterococci were reported in chickens, turkeys, ducks and ostriches. The aim of this study was to evaluate the occurrence of enterococci in poultry in Poland, including identification of enterococcus species composition and determination of the age of birds. The analysis was based on data obtained from 2014 – 2015 from Division of Avian Diseases at Warsaw University of Life

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KIM, YEONG BIN, HYUN JOO SEO, KWANG WON SEO, et al. "Characteristics of High-Level Ciprofloxacin-Resistant Enterococcus faecalis and Enterococcus faecium from Retail Chicken Meat in Korea." Journal of Food Protection 81, no. 8 (2018): 1357–63. http://dx.doi.org/10.4315/0362-028x.jfp-18-046.

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ABSTRACTGenes encoding ciprofloxacin resistance in enterococci in animals may be transferred to bacteria in the animal gut and to zoonotic bacteria where they could pose a human health hazard. The objective of this study was to characterize antimicrobial resistance in high-level ciprofloxacin-resistant (HLCR) Enterococcus faecalis and Enterococcus faecium isolated from retail chicken meat. A total of 345 enterococci (335 E. faecalis and 10 E. faecium) were isolated from 200 chicken meat samples. Of these, 85 E. faecalis isolates and 1 E. faecium isolate were confirmed as HLCR enterococci. All

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Singh, Dr Kapil, Dr Charu Jain, and Dr Shukla Das. "BURDEN OF ENTEROCOCCAL ISOLATES FROM CSF IN SUSPECTED CASES OF MENINGITIS IN A TERTIARY CARE HOSPITAL, IN EAST DELHI." International Journal of Engineering Applied Sciences and Technology 9, no. 07 (2024): 32–34. https://doi.org/10.33564/ijeast.2024.v09i07.004.

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Pediatric meningitis pathogens like Enterococcus faecalis and faecium are rare but important. Unlike Streptococcus pneumoniae and Neisseria meningitidis, which cause most bacterial meningitis in children, enterococcal meningitis is resistant and difficult to treat. Any disturbance in host/commensal homeostasis that compromises the host defense mechanism or environmental influences, such as antibiotic use that promotes resistant enterococci, can cause life-threatening infections. High-level aminoglycoside resistance (HLAR) and vancomycin-resistant enterococci (VRE) in clinical isolates are caus

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Tsiodras, Sotirios, Howard S. Gold, Eoin P. G. Coakley, Christine Wennersten, Robert C. Moellering, and George M. Eliopoulos. "Diversity of Domain V of 23S rRNA Gene Sequence in Different Enterococcus Species." Journal of Clinical Microbiology 38, no. 11 (2000): 3991–93. http://dx.doi.org/10.1128/jcm.38.11.3991-3993.2000.

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The highly conserved central loop of domain V of 23S RNA (nucleotides 2042 to 2628; Escherichia coli numbering) is implicated in peptidyltransferase activity and represents one of the target sites for macrolide, lincosamide, and streptogramin B antibiotics. DNA encoding domain V (590 bp) of several species ofEnterococcus was amplified by PCR. Twenty enterococcal isolates were tested, including Enterococcus faecium (six isolates), Enterococcus faecalis, Enterococcus avium, Enterococcus durans, Enterococcus gallinarum, Enterococcus casseliflavus (two isolates of each), and Enterococcus raffinosu

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Jett, Bradley D., Rajeshwari V. Atkuri, and Michael S. Gilmore. "Enterococcus faecalis Localization in Experimental Endophthalmitis: Role of Plasmid-Encoded Aggregation Substance." Infection and Immunity 66, no. 2 (1998): 843–48. http://dx.doi.org/10.1128/iai.66.2.843-848.1998.

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ABSTRACT Enterococci have emerged as leading agents of nosocomial infection, yet relatively little is known about the pathogenesis of enterococcal disease. In previous studies, we developed an Enterococcus faecalis endophthalmitis infection model which provides unique opportunities to study the evolution of enterococcal disease by direct observation, as well as through sensitive electrophysiologic measures of organ function. The present study was designed to determine whetherE. faecalis possesses traits that permit its attachment to mammalian tissues during infection. It was also of interest t

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Figueras, M. J., I. Inza, F. Polo, and J. Guarro. "Evaluation of the oxolinic acid - esculin - azide medium for the isolation and enumeration of faecal streptococci in a routine monitoring programme for bathing waters." Canadian Journal of Microbiology 44, no. 10 (1998): 998–1002. http://dx.doi.org/10.1139/w98-096.

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m-Enterococcus agar (m-Ent) has been generally considered the reference medium for faecal streptococci in bathing waters. However, it shows several shortcomings, and therefore it is important to test newly developed media that can guarantee more precise results. In this sense, the recently described oxolinic acid - esculin - azide agar medium (OAA) and m-enterococccus agar (m-Ent) were comparatively evaluated for the detection of faecal streptococci from seawater and fresh water. The OAA medium showed a significantly higher relative recovery percentage and specificity for both types of water t

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Gelsomino, Roberto, Marc Vancanneyt, Timothy M. Cogan, and Jean Swings. "Effect of Raw-Milk Cheese Consumption on the Enterococcal Flora of Human Feces." Applied and Environmental Microbiology 69, no. 1 (2003): 312–19. http://dx.doi.org/10.1128/aem.69.1.312-319.2003.

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ABSTRACT Enterococci are one of the major facultative anaerobic bacterial groups that reside in the human gastrointestinal tract. In the present study, the composition of the enterococcal fecal flora in three healthy humans was analyzed before, during, and after the daily consumption of ∼125 g of a raw-milk Cheddar-type cheese containing 3.2 × 104 enterococci/g of cheese. Enterococcal counts ranged between 1.4 × 102 and 2.5 × 108 CFU/g of feces and differed from subject to subject and from week to week. The cheese contained mainly Enterococcus casseliflavus and a small population of Enterococc

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Paul, Manisha, Prem Singh Nirwan, and Preeti Srivastava. "Detection of high-level aminoglycoside resistance by disc diffusion and e-test amongst the enterococcus species isolated from various clinical samples in a tertiary care hospital." International Journal of Research in Medical Sciences 7, no. 9 (2019): 3527. http://dx.doi.org/10.18203/2320-6012.ijrms20193941.

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Background: The emergence of Enterococcus species in causing nosocomial infections poses a therapeutic challenge to clinicians. Enterococci are intrinsically resistance to multiple antibiotics. Acquired resistance to commonly used antibiotics like Ampicillin, Vancomycin and Aminoglycosides have made the situation worse and difficult to treat serious Enterococcal infections. The present study aimed at detection of high-level aminoglycoside resistance by disc diffusion and E-test amongst the Enterococcus species isolated from various clinical samples in a tertiary care hospital.Methods: A total

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Carrasco-Acosta, Marina, and Pilar Garcia-Jimenez. "Development of Multiplex RT qPCR Assays for Simultaneous Detection and Quantification of Faecal Indicator Bacteria in Bathing Recreational Waters." Microorganisms 12, no. 6 (2024): 1223. http://dx.doi.org/10.3390/microorganisms12061223.

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In this study, we designed and validated in silico and experimentally a rapid, sensitive, and specific multiplex RT qPCR for the detection and quantification of faecal indicator bacteria (FIB) used as microbiological references in marine bathing water regulations (Escherichia coli and intestinal enterococci). The 16S rRNA gene was used to quantify group-specific enterococci and Escherichia/Shigella and species-specific such as Enterococcus faecalis and E. faecium. Additionally, a ybbW gene encoding allantoin transporter protein was used to detect E. coli. An assessment of marine coastal system

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G., Hemalatha, Bhaskaran K., Sowmiya M., Anusheela Howlader, and Sethumadhavan K. "A study on virulence factors and antimicrobial resistance pattern among enterococci isolated from various clinical specimens from a tertiary care hospital." International Journal of Research in Medical Sciences 5, no. 7 (2017): 2969. http://dx.doi.org/10.18203/2320-6012.ijrms20172971.

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Background: Enterococci, adult faeces commensal are important nosocomial pathogens. E. faecalis is the most common cause of infection, followed by E. faecium. In the past two decades, they have developed resistance to many commonly used antimicrobial agents. Understanding virulence factors and monitoring antimicrobial resistance among Enterococci is essential for controlling the spread of bacterial resistance and important for epidemiological surveillance within the hospital environment. The aim of the study is to evaluate antibiotic resistance and virulence factors exhibited by Enterococcus s

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KuKanich, Kate S., and Brian V. Lubbers. "Review of Enterococci Isolated from Canine and Feline Urine Specimens from 2006 to 2011." Journal of the American Animal Hospital Association 51, no. 3 (2015): 148–54. http://dx.doi.org/10.5326/jaaha-ms-6070.

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Canine and feline urine culture reports and medical records were reviewed at a veterinary teaching hospital from 2006 to 2011 for enterococcal growth, coinfections, antimicrobial resistance, urine sediment findings, clinical signs, and concurrent conditions. Of all of the urine specimens with significantly defined colony-forming units/mL, Enterococcus (E.) faecalis was the only enterococci isolated from cats and predominated (77.4%) in dogs followed by E. faecium (12.9%), E. durans (3.2%), and other Enterococcus spp. (6.5%). The majority of specimens with significant enterococcal growth result

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KOBAYASHI, N., MD MAHBUB ALAM, Y. NISHIMOTO, S. URASAWA, N. UEHARA, and N. WATANABE. "Distribution of aminoglycoside resistance genes in recent clinical isolates of Enterococcus faecalis, Enterococcus faecium and Enterococcus avium." Epidemiology and Infection 126, no. 2 (2001): 197–204. http://dx.doi.org/10.1017/s0950268801005271.

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Aminoglycoside modifying enzymes (AMEs) are major factors which confer aminoglycoside resistance on bacteria. Distribution of genes encoding seven AMEs was investigated by multiplex PCR for 279 recent clinical isolates of enterococci derived from a university hospital in Japan. The aac(6′)-aph(2″), which is related to high level gentamicin resistance, was detected at higher frequency in Enterococcus faecalis (42·5 %) than in Enterococcus faecium (4·3 %). Almost half of E. faecalis and E. faecium isolates possessed ant(6)-Ia and aph(3′)-IIIa. The profile of AME gene(s) detected most frequently

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MARTÍN, MARÍA, JORGE GUTIÉRREZ, RAQUEL CRIADO, CARMEN HERRANZ, LUIS M. CINTAS, and PABLO E. HERNÁNDEZ. "Genes Encoding Bacteriocins and Their Expression and Potential Virulence Factors of Enterococci Isolated from Wood Pigeons (Columba palumbus)." Journal of Food Protection 69, no. 3 (2006): 520–31. http://dx.doi.org/10.4315/0362-028x-69.3.520.

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Samples of the intestinal content and carcasses of wood pigeons (Columba palumbus) were evaluated for enterococci with antimicrobial activity. Enterococcus faecium comprised the largest enterococcal species with antagonistic activity, followed by Enterococcus faecalis and Enterococcus columbae. PCR amplification of genes coding bacteriocins and determination of their nucleotide sequence, and the use of specific antipeptide bacteriocin antibodies and a noncompetitive indirect enzyme-linked immunosorbent assay, permitted characterization of enterococci coding that described bacteriocins and thei

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Zerbato, Verena, Riccardo Pol, Gianfranco Sanson, et al. "Risk Factors for 30-Day Mortality in Nosocomial Enterococcal Bloodstream Infections." Antibiotics 13, no. 7 (2024): 601. http://dx.doi.org/10.3390/antibiotics13070601.

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Enterococci commonly cause nosocomial bloodstream infections (BSIs), and the global incidence of vancomycin-resistant enterococci (VRE) BSIs is rising. This study aimed to assess the risk factors for enterococcal BSIs and 30-day mortality, stratified by Enterococcus species, vancomycin resistance, and treatment appropriateness. We conducted a retrospective cohort study (2014–2021) including all hospitalized adult patients with at least one blood culture positive for Enterococcus faecalis or Enterococcus faecium. We included 584 patients with enterococcal BSI: 93 were attributed to vancomycin-r

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Su, Rina, Yunzhi Peng, Zhanli Wang, Hui Yu, and Qi Wu. "Identification of two novel type II topoisomerase mutations in Enterococcus spp. isolated from a hospital in China." Archives of Biological Sciences, no. 00 (2021): 34. http://dx.doi.org/10.2298/abs210628034s.

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Type II topoisomerases, including DNA gyrase (GyrA) and topoisomerase IV (ParC), contribute to fluoroquinolone resistance in Enterococcus spp. This study investigated the mutational status of the quinolone resistance-determining regions (QRDRs) of GyrA and ParC in the clinical isolates of enterococci from a hospital in Baotou, China. We analyzed 110 enterococcal isolates, including 57 Enterococcus faecalis and 53 Enterococcus faecalis faecium. The resistance rates of E. faecalis and E. faecium to ciprofloxacin were 63.16% and 84.91%, respectively. We found that 32 samples of E. faecalis and 42

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Bera, Shyamsundar, Sonia Mehta, Manisha Bhatt Dwivedi, Varsha A. Singh, Rajdeep Paul, and Sumi Nongrum. "Study on vancomycin-resistant enterococci in faecal samples from non-hospitalized individuals at MMIMSR, Haryana, India." Bangladesh Journal of Medical Science 18, no. 2 (2019): 334–39. http://dx.doi.org/10.3329/bjms.v18i2.40705.

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Background: Enterococci, formerly classified with fecal streptococci, have been recognized to be of fecal origin since the beginning of this century. Method: This study was undertaken to determine the prevalence of stool colonization with vancomycin resistant Enterococcus (VRE) and also to evaluate the risk factors for colonization with vancomycin resistant Enterococcus among non- Hospitalized individuals at MMIMSR, Mullana.Test was performed for VRE isolates collected over a period of 6 months (Oct2015- March 2016). Faecal samples were collected by using sterile container from non- hosp

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Gaspar, Frédéric, Neuza Teixeira, Lionel Rigottier-Gois, et al. "Virulence of Enterococcus faecalis dairy strains in an insect model: the role of fsrB and gelE." Microbiology 155, no. 11 (2009): 3564–71. http://dx.doi.org/10.1099/mic.0.030775-0.

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Despite the existence of various virulence factors in the Enterococcus genus, enterococcal virulence is still a debated issue. A main consideration is the detection of the same virulence genes in strains isolated from nosocomial or community-acquired infections, and from food products. The goal of this study was to evaluate the roles of two well-characterized enterococcal virulence factors, Fsr and gelatinase, in the potential virulence of Enterococcus faecalis food strains. Virulence of unrelated Enterococcus isolates, including dairy strains carrying fsr and gelE operons, was compared in the

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Krawczyk, Beata, Paweł Wityk, Mirosława Gałęcka, and Michał Michalik. "The Many Faces of Enterococcus spp.—Commensal, Probiotic and Opportunistic Pathogen." Microorganisms 9, no. 9 (2021): 1900. http://dx.doi.org/10.3390/microorganisms9091900.

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Enterococcus spp. are Gram-positive, facultative, anaerobic cocci, which are found in the intestinal flora and, less frequently, in the vagina or mouth. Enterococcus faecalis and Enterococcus faecium are the most common species found in humans. As commensals, enterococci colonize the digestive system and participate in the modulation of the immune system in humans and animals. For many years reference enterococcal strains have been used as probiotic food additives or have been recommended as supplements for the treatment of intestinal dysbiosis and other conditions. The use of Enterococcus str

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Lin, Yu-Tzu, Sung-Pin Tseng, Wei-Wen Hung, et al. "A Possible Role of Insertion Sequence IS1216V in Dissemination of Multidrug-Resistant Elements MESPM1 and MES6272-2 between Enterococcus and ST59 Staphylococcus aureus." Microorganisms 8, no. 12 (2020): 1905. http://dx.doi.org/10.3390/microorganisms8121905.

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Sequence type 59 (ST59) is the dominant type of community-associated methicillin-resistant Staphylococcus aureus (MRSA) in Taiwan. Previously, we reported that ST59 MRSA harbors enterococcal IS1216V-mediated multidrug-resistant composite transposons MESPM1 or MES6272-2. The MES were found to have a mosaic structure, largely originating in enterococci and partly native to S. aureus. The current study aimed to track the origin of the MES and how they disseminated from enterococci to ST59 S. aureus. A total of 270 enterococcal isolates were analyzed, showing that two ST64 Enterococcus faecalis is

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Gião, Joana, Célia Leão, Teresa Albuquerque, Lurdes Clemente, and Ana Amaro. "Antimicrobial Susceptibility of Enterococcus Isolates from Cattle and Pigs in Portugal: Linezolid Resistance Genes optrA and poxtA." Antibiotics 11, no. 5 (2022): 615. http://dx.doi.org/10.3390/antibiotics11050615.

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Enterococci are part of the commensal gut microbiota of mammals, with Enterococcus faecalis and Enterococcus faecium being the most clinically relevant species. This study assesses the prevalence and diversity of enterococcal species in cattle (n = 201) and pig (n = 249) cecal samples collected in 2017. Antimicrobial susceptibility profiles of E. faecium (n = 48) and E. faecalis (n = 84) were assessed by agar and microdilution methods. Resistance genes were screened through PCR and nine strains were analyzed by Whole Genome Sequencing. A wide range of enterococci species was found colonizing t

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Goel, Varun, Dinesh Kumar, Rajendra Kumar, Purva Mathur, and Sarman Singh. "Community Acquired Enterococcal Urinary Tract Infections and Antibiotic Resistance Profile in North India." Journal of Laboratory Physicians 8, no. 01 (2016): 050–54. http://dx.doi.org/10.4103/0974-2727.176237.

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ABSTRACT Background: Urinary tract infections (UTIs) remain a major problem both in hospitalized and outdoor patients. Multidrug-resistant enterococci are emerging as a major nosocomial pathogen with increasing frequency. However, the incidence of community-acquired enterococcal infections and species prevalent in India is not thoroughly investigated. Objectives: This study aims to estimate the burden of community-acquired UTIs seen at a tertiary care hospital and to identify the Enterococcus species isolated from these patients. The study also aims to determine the antibiotic susceptibility p

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CHINGWARU, W., S. F. MPUCHANE, and B. A. GASHE. "Enterococcus faecalis and Enterococcus faecium Isolates from Milk, Beef, and Chicken and Their Antibiotic Resistance." Journal of Food Protection 66, no. 6 (2003): 931–36. http://dx.doi.org/10.4315/0362-028x-66.6.931.

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The occurrence and antibiotic resistance of enterococci, especially Enterococcus faecalis and Enterococcus faecium, in milk, beef, and chicken in Gaborone, Botswana, were studied. Enterococci were isolated from these sources with the use of bile esculin agar and identified with API 20 Strep kits. Antibiotic resistance was determined by the disk diffusion method. The antibiotics tested were vancomycin, teicoplanin, ampicillin, tetracycline, and cephalothin. Among the 1,467 enterococci isolated from the samples, E. faecalis (46.1%) and E. faecium (29.0%) were found to be the predominant species.

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Ke, Danbing, Maurice Boissinot, Ann Huletsky, et al. "Evidence for Horizontal Gene Transfer in Evolution of Elongation Factor Tu in Enterococci." Journal of Bacteriology 182, no. 24 (2000): 6913–20. http://dx.doi.org/10.1128/jb.182.24.6913-6920.2000.

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ABSTRACT The elongation factor Tu, encoded by tuf genes, is a GTP binding protein that plays a central role in protein synthesis. One to three tuf genes per genome are present, depending on the bacterial species. Most low-G+C-content gram-positive bacteria carry only one tuf gene. We have designed degenerate PCR primers derived from consensus sequences of the tuf gene to amplify partial tuf sequences from 17 enterococcal species and other phylogenetically related species. The amplified DNA fragments were sequenced either by direct sequencing or by sequencing cloned inserts containing putative

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Komenkova, T. S., and E. A. Zaitseva. "Modern View on Enterococcus faecalis and Enterococcus faecium Resistance Mechanisms to Antibiotics." Antibiotics and Chemotherapy 65, no. 11-12 (2021): 38–48. http://dx.doi.org/10.37489/0235-2990-2020-65-11-12-38-48.

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Enterococci are currently becoming one of the major causative agents of various infectious diseases. Enterococcus faecalis and E.faecium are the most common species causing enterococcal infections. Both species exhibit natural low-level resistance to aminoglycosides, cephalosporins, quinolones, clindamycin, and co-trimoxazole. In addition, the peculiarities of their genome make it easy to acquire resistance to other antibiotics widely used in clinical practice, through mutations or by horizontal gene transfer. The review represents current knowledge about the mechanisms of enterococcal resista

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Shepard, Brett D., and Michael S. Gilmore. "Differential Expression of Virulence-Related Genes in Enterococcus faecalis in Response to Biological Cues in Serum and Urine." Infection and Immunity 70, no. 8 (2002): 4344–52. http://dx.doi.org/10.1128/iai.70.8.4344-4352.2002.

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ABSTRACT Enterococci rank among leading causes of nosocomial bacteremia and urinary tract infection and are also a leading cause of community acquired subacute endocarditis. Limited evidence suggests that biological cues in serum and urine may play an important role in modulating enterococcal virulence at sites of infection. To determine the extent to which biological cues affect enterococcal virulence-associated gene expression, we used quantitative real-time PCR to compare mRNA levels in Enterococcus faecalis cultures grown in serum or urine to that achieved in laboratory medium. Both enviro

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Paterson, David, Janet Bodman, and Mee len Thong. "High level aminoglycoside resistance in enterococcal blood culture isolates." Communicable Diseases Intelligence 20 (December 9, 1996): 532–35. https://doi.org/10.33321/cdi.1996.20.87.

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Enterococci may display high level resistance to aminoglycosides, in which case synergy with cell-wall active antibiotics will be lost. All enterococcal blood culture isolates at Royal Brisbane Hospital have been screened by agar dilution for high level resistance to gentamicin and streptomycin since 1989. Of 110 isolates of Enterococcus faecalis, 16% displayed high level resistance togentamicin and 10% showed high level resistance to streptomycin. Four isolates had high level resistance to both antibiotics. None of 23 Enterococcus faecium isolates displayed high level resistance to gentamicin

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Huescas, C. G. Y., R. I. Pereira, J. Prichula, P. A. Azevedo, J. Frazzon, and A. P. G. Frazzon. "Frequency of Clustered Regularly Interspaced Short Palindromic Repeats (CRISPRs) in non-clinical Enterococcus faecalis and Enterococcus faecium strains." Brazilian Journal of Biology 79, no. 3 (2019): 460–65. http://dx.doi.org/10.1590/1519-6984.183375.

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Abstract The fidelity of the genomes is defended by mechanism known as Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) systems. Three Type II CRISPR systems (CRISPR1- cas, CRISPR2 and CRISPR3-cas) have been identified in enterococci isolates from clinical and environmental samples. The aim of this study was to observe the distribution of CRISPR1-cas, CRISPR2 and CRISPR3-cas in non-clinical strains of Enterococcus faecalis and Enterococcus faecium isolates from food and fecal samples, including wild marine animals. The presence of CRISPRs was evaluated by PCR in 120 enterococ

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Jonas, Brandie M., Barbara E. Murray, and George M. Weinstock. "Characterization of emeA, anorA Homolog and Multidrug Resistance Efflux Pump, inEnterococcus faecalis." Antimicrobial Agents and Chemotherapy 45, no. 12 (2001): 3574–79. http://dx.doi.org/10.1128/aac.45.12.3574-3579.2001.

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ABSTRACT We hypothesized that multidrug resistance efflux pumps (MDRs) may be contributing to the drug resistance of enterococci. We recently identified potential MDR-encoding genes in the Enterococcus faecalis V583 genome. Among the putative MDRs, we found a gene that encodes a NorA homolog and have characterized this enterococcal MDR in the present study. A mutant from which the enterococcal NorA homolog has been deleted has reduced resistance to several NorA substrates. Complementation of the deletion mutant with the wild-type gene verified the involvement of this enterococcal gene in resis

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Romero-Saavedra, F., D. Laverde, E. Kalfopoulou, et al. "Conjugation of Different Immunogenic Enterococcal Vaccine Target Antigens Leads to Extended Strain Coverage." Journal of Infectious Diseases 220, no. 10 (2019): 1589–98. http://dx.doi.org/10.1093/infdis/jiz357.

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Abstract Enterococci have emerged as important nosocomial pathogens due to their resistance to the most commonly used antibiotics. Alternative treatments or prevention options are aimed at polysaccharides and surface-related proteins that play important roles in pathogenesis. Previously, we have shown that 2 Enterococcus faecium proteins, the secreted antigen A and the peptidyl-prolyl cis-trans isomerase, as well as the Enterococcus faecalis polysaccharide diheteroglycan, are able to induce opsonic and cross-protective antibodies. Here, we evaluate the use of glycoconjugates consisting of thes

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Huebner, Johannes, Alexander Quaas, Wolfgang A. Krueger, Donald A. Goldmann, and Gerald B. Pier. "Prophylactic and Therapeutic Efficacy of Antibodies to a Capsular Polysaccharide Shared among Vancomycin-Sensitive and -Resistant Enterococci." Infection and Immunity 68, no. 8 (2000): 4631–36. http://dx.doi.org/10.1128/iai.68.8.4631-4636.2000.

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ABSTRACT Enterococci are important nosocomial pathogens that are increasingly difficult to treat due to intrinsic and acquired resistance to antibiotics, including vancomycin. A recently described capsular polysaccharide (CP) isolated from Enterococcus faecalis 12030 was used to evaluate the potential efficacy of active or passive immunotherapy regimens as adjunctive treatments. Evaluation of protective efficacy was carried out in immunocompetent mice challenged intravenously (i.v.) with live enterococci. In nonimmune mice, i.v. inoculations resulted in high levels of bacteria in kidneys, sple

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Palmer, Kelli L., Karen Carniol, Janet M. Manson, et al. "High-Quality Draft Genome Sequences of 28 Enterococcus sp. Isolates." Journal of Bacteriology 192, no. 9 (2010): 2469–70. http://dx.doi.org/10.1128/jb.00153-10.

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ABSTRACT The enterococci are low-GC Gram-positive bacteria that have emerged as leading causes of hospital-acquired infection. They are also commensals of the gastrointestinal tract of healthy humans and most other animals with gastrointestinal flora and are important for food fermentations. Here we report the availability of draft genome sequences for 28 enterococcal strains of diverse origin, including the species Enterococcus faecalis, E. faecium, E. casseliflavus, and E. gallinarum.

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Macovei, Lilia, and Ludek Zurek. "Ecology of Antibiotic Resistance Genes: Characterization of Enterococci from Houseflies Collected in Food Settings." Applied and Environmental Microbiology 72, no. 6 (2006): 4028–35. http://dx.doi.org/10.1128/aem.00034-06.

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ABSTRACT In this project, enterococci from the digestive tracts of 260 houseflies (Musca domestica L.) collected from five restaurants were characterized. Houseflies frequently (97% of the flies were positive) carried enterococci (mean, 3.1 ï¿½ 103 CFU/fly). Using multiplex PCR, 205 of 355 randomly selected enterococcal isolates were identified and characterized. The majority of these isolates were Enterococcus faecalis (88.2%); in addition, 6.8% were E. faecium, and 4.9% were E. casseliflavus. E. faecalis isolates were phenotypically resistant to tetracycline (66.3%), erythromycin (23.8%), st

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Surbhi, Suneja, Lalwani Jaya, Shrivastava Rakesh, and Chaurasia Deepti. "Characterization and detection of Linezolid resistance in clinical isolates of Enterococci in Tertiary care Hospital of Central India." International Journal of Pharmaceutical and Clinical Research 15, no. 8 (2023): 1549–54. https://doi.org/10.5281/zenodo.11528544.

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<strong>Introduction:</strong> The prevalence of antibiotic resistance within the Enterococcus species is on the rise. <strong>Aim:</strong> This study aimed to ascertain the occurrence of linezolid-resistance amongst the Enterococcal bacterial specimens isolated from patients. <strong>Materials and Methods:</strong> This was a single centre, hospital; laboratory based clinical cross-sectional study conducted over a period of 18 months. The Enterococcus bacteria isolated and identified from diverse samples specimen were subjected to culture and antibiotic sensitivity t

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Adeniji, Oluwaseun Ola, Nolonwabo Nontongana, and Anthony Ifeanyin Okoh. "Prevalence of Class 1 Integron and In Vitro Effect of Antibiotic Combinations of Multidrug-Resistant Enterococcus Species Recovered from the Aquatic Environment in the Eastern Cape Province, South Africa." International Journal of Molecular Sciences 24, no. 3 (2023): 2993. http://dx.doi.org/10.3390/ijms24032993.

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Enterococci are regarded as a better indication of faecal pollution in freshwater and marine waters. Their levels in seawater are positively connected with swimming-related gastrointestinal disorders. This study used an Enterococcus-specific polymerase chain reaction (PCR) to characterize the isolates. Classes 1 and 2 integrons were examined for environmental Enterococcus isolates using a standard biological procedure. All strains were assessed against a panel of 12 antibiotics from various classes using disc diffusion methods. The microdilution method was used to work out the minimum inhibito

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Andrews Jr., Robert E., Wesley S. Johnson, Abby R. Guard, and Jonathan D. Marvin. "Survival of enterococci and Tn916-like conjugative transposons in soil." Canadian Journal of Microbiology 50, no. 11 (2004): 957–66. http://dx.doi.org/10.1139/w04-090.

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The persistence of Enterococcus faecalis, fecal enterococci from swine waste, and Tn916-like elements was determined following inoculation into autoclaved and native soil microcosms. When cells of E. faecalis CG110 (Tn916) were inoculated into native microcosms, enterococcal viability in the soil decreased approximately 5 orders of magnitude (4.8 × 105CFU/g soil to < 10 CFU/g) after 5 weeks. In autoclaved microcosms, the viability of E. faecalis decreased by only 20% in 5 weeks. In contrast, the content of Tn916, based on PCR of DNA extracts from soil microcosms, decreased by about 20% in b

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Min, Yu-Hong, Yong-ung Kim, and Min Chul Park. "In Vitro Synergistic Effect of Lefamulin with Doxycycline, Rifampin, and Quinupristin/Dalfopristin Against Enterococci." Microorganisms 12, no. 12 (2024): 2515. https://doi.org/10.3390/microorganisms12122515.

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The widespread resistance of enterococci to many commonly used antimicrobial agents is a growing concern. Given that the current treatment options for enterococcal infections are limited, the discovery of new therapies, including combination therapies, is necessary. We evaluated double-drug combinations of lefamulin with doxycycline, rifampin, and quinupristin/dalfopristin for in vitro synergy against strains of Enterococcus faecium (E. faecium) and Enterococcus faecalis (E. faecalis) by using checkerboard and time-kill assays. In the checkerboard assay, the synergistic effect of lefamulin wit

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Alexandrova, Natalya A., Maya I. Zaslavskaya, Irina V. Soloveva, Anna G. Tochilina, and Irina V. Belova. "EVALUATION OF ANTI-CANDIDA ACTIVITY OF METABOLITES OF ENTEROCOCCAL CLINICAL ISOLATES." Russian Clinical Laboratory Diagnostics 64, no. 11 (2019): 690–92. http://dx.doi.org/10.18821/0869-2084-2019-64-11-690-692.

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When studying the effect of the metabolic products of clinical isolates of enterococci on the viability of Candida albicans, it was found that metabolites of all tested strains of Enterococcus faecium, E. faecalis had a fungistatic effect. At the same time a reliable fungicidal effect is a strain-specific feature. It is better to use the method of delayed antagonism on double-layer agar to assess the antifungal effect of enterococcal metabolism products.

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Yangzom, Tsering, and T. Shanti Kumar Singh. "Study of vancomycin and high-level aminoglycoside-resistant Enterococcus species and evaluation of a rapid spot test for enterococci from Central Referral Hospital, Sikkim, India." Journal of Laboratory Physicians 11, no. 03 (2019): 192–99. http://dx.doi.org/10.4103/jlp.jlp_5_19.

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Abstract BACKGROUND: Enterococcus is an important pathogen, and with its emergence of resistance to multiple antimicrobials, the management of infection is becoming increasingly difficult. AIM: The aim of the study is to determine the prevalence, antibiotic resistance, and risk factors associated with enterococcal infection or colonization. MATERIALS AND METHODS: In this prospective study, samples from inpatients were screened for resistant enterococci. Antibiotic susceptibility testing was performed using the disc diffusion method and minimum inhibitory concentration by the agar dilution meth

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Baldassarri, L., R. Creti, L. Montanaro, G. Orefici, and C. R. Arciola. "Pathogenesis of Implant Infections by Enterococci." International Journal of Artificial Organs 28, no. 11 (2005): 1101–9. http://dx.doi.org/10.1177/039139880502801107.

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Enterococci are commensals of human and animal intestinal tract that have emerged in the last decades as a major cause of nosocomial infections of bloodstream, urinary tract and in infected surgical sites. Enterococcus faecalis is responsible for ca. 80% of all enterococcal infections while Enterococcus faecium accounts for most of the others; among the most relevant risk factors for development of enterococcal infections is the presence of implanted devices. The pathogenesis of such infections is poorly understood, but several virulence factors have been proposed. Among them, the ability to f

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Khairy, Rasha MM, Mahmoud Shokry Mahmoud, Mona Abdel Monem Esmail, and Aya Nabil Gamil. "First detection of vanB phenotype-vanA genotype vancomycin-resistant enterococci in Egypt." Journal of Infection in Developing Countries 13, no. 09 (2019): 837–42. http://dx.doi.org/10.3855/jidc.10472.

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Introduction: Enterococci have emerged in last two decades as serious hospital acquired pathogens particularly vancomycin resistant strains (VRE). The study aimed to identify the prevalence of enterococcal isolation from hospital infections and colonization as well as determine vancomycin resistance phenotypes and genotypes. Methods: Sixty enterococcus isolates were isolated from patients, health care workers and hospital environment, identified and tested for antimicrobial susceptibility. Enterococcus species were identified by Real-time PCR and vancomycin resistance was assessed by agar dilu

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Hayes, Joshua R., Linda L. English, Peggy J. Carter, et al. "Prevalence and Antimicrobial Resistance of Enterococcus Species Isolated from Retail Meats." Applied and Environmental Microbiology 69, no. 12 (2003): 7153–60. http://dx.doi.org/10.1128/aem.69.12.7153-7160.2003.

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ABSTRACT From March 2001 to June 2002, a total of 981 samples of retail raw meats (chicken, turkey, pork, and beef) were randomly obtained from 263 grocery stores in Iowa and cultured for the presence of Enterococcus spp. A total of 1,357 enterococcal isolates were recovered from the samples, with contamination rates ranging from 97% of pork samples to 100% of ground beef samples. Enterococcus faecium was the predominant species recovered (61%), followed by E. faecalis (29%), and E. hirae (5.7%). E. faecium was the predominant species recovered from ground turkey (60%), ground beef (65%), and

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TEMPLER, S. P., P. ROHNER, and A. BAUMGARTNER. "Relation of Enterococcus faecalis and Enterococcus faecium Isolates from Foods and Clinical Specimens." Journal of Food Protection 71, no. 10 (2008): 2100–2104. http://dx.doi.org/10.4315/0362-028x-71.10.2100.

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Clinical Enterococcus faecalis (n = 65) and Enterococcus faecium (n = 12) blood isolates from three Swiss hospitals were characterized with testing for resistance to antimicrobial agents, pulsed-field gel electrophoresis (PFGE), and the occurrence of virulence factors. Phenotypic determination of resistance to antimicrobial agents resulted in 20% of E. faecalis isolates showing a triple resistance against chloramphenicol, tetracycline, erythromycin, and seven isolates (two E. faecalis and five E. faecium) exhibiting a multiresistance against five or more antimicrobials. One isolate each of E.

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