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Littérature scientifique sur le sujet « Filamentous phage »

Auteur : Grafiati

Publié le 12 avril 2025

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Articles de revues sur le sujet "Filamentous phage"

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Barbas, S. M., et C. F. Barbas. « Filamentous phage display ». Fibrinolysis 8 (janvier 1994) : 245–52. http://dx.doi.org/10.1016/0268-9499(94)90722-6.

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Cairns, Johannes, Sebastián Coloma, Kaarina Sivonen et Teppo Hiltunen. « Evolving interactions between diazotrophic cyanobacterium and phage mediate nitrogen release and host competitive ability ». Royal Society Open Science 3, n^o 12 (décembre 2016) : 160839. http://dx.doi.org/10.1098/rsos.160839.

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Interactions between nitrogen-fixing (i.e. diazotrophic) cyanobacteria and their viruses, cyanophages, can have large-scale ecosystem effects. These effects are mediated by temporal alterations in nutrient availability in aquatic systems owing to the release of nitrogen and carbon sources from cells lysed by phages, as well as by ecologically important changes in the diversity and fitness of cyanobacterial populations that evolve in the presence of phages. However, ecological and evolutionary feedbacks between phages and nitrogen-fixing cyanobacteria are still relative poorly understood. Here, we used an experimental evolution approach to test the effect of interactions between a common filamentous, nitrogen-fixing cyanobacterium ( Nodularia sp.) and its phage on cellular nitrogen release and host properties. Ecological, community-level effects of phage-mediated nitrogen release were tested with a phytoplankton bioassay. We found that cyanobacterial nitrogen release increased significantly as a result of viral lysis, which was associated with enhanced growth of phytoplankton species in cell-free filtrates compared with phage-resistant host controls in which lysis and subsequent nutrient release did not occur after phage exposure. We also observed an ecologically important change among phage-evolved cyanobacteria with phage-resistant phenotypes, a short-filamentous morphotype with reduced buoyancy compared with the ancestral long-filamentous morphotype. Reduced buoyancy might decrease the ability of these morphotypes to compete for light compared with longer, more buoyant filaments. Together, these findings demonstrate the potential of cyanobacteria–phage interactions to affect ecosystem biogeochemical cycles and planktonic community dynamics.

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Chibani, Cynthia Maria, Robert Hertel, Michael Hoppert, Heiko Liesegang et Carolin Charlotte Wendling. « Closely Related Vibrio alginolyticus Strains Encode an Identical Repertoire of Caudovirales-Like Regions and Filamentous Phages ». Viruses 12, n^o 12 (27 novembre 2020) : 1359. http://dx.doi.org/10.3390/v12121359.

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Many filamentous vibriophages encode virulence genes that lead to the emergence of pathogenic bacteria. Most genomes of filamentous vibriophages characterized up until today were isolated from human pathogens. Despite genome-based predictions that environmental Vibrios also contain filamentous phages that contribute to bacterial virulence, empirical evidence is scarce. This study aimed to characterize the bacteriophages of a marine pathogen, Vibrio alginolyticus (Kiel-alginolyticus ecotype) and to determine their role in bacterial virulence. To do so, we sequenced the phage-containing supernatant of eight different V. alginolyticus strains, characterized the phages therein and performed infection experiments on juvenile pipefish to assess their contribution to bacterial virulence. We were able to identify two actively replicating filamentous phages. Unique to this study was that all eight bacteria of the Kiel-alginolyticus ecotype have identical bacteriophages, supporting our previously established theory of a clonal expansion of the Kiel-alginolyticus ecotype. We further found that in one of the two filamentous phages, two phage-morphogenesis proteins (Zot and Ace) share high sequence similarity with putative toxins encoded on the Vibrio cholerae phage CTXΦ. The coverage of this filamentous phage correlated positively with virulence (measured in controlled infection experiments on the eukaryotic host), suggesting that this phage contributes to bacterial virulence.

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Faruque, Shah M., Iftekhar Bin Naser, Kazutaka Fujihara, Pornphan Diraphat, Nityananda Chowdhury, M. Kamruzzaman, Firdausi Qadri, Shinji Yamasaki, A. N. Ghosh et John J. Mekalanos. « Genomic Sequence and Receptor for the Vibrio cholerae Phage KSF-1Φ : Evolutionary Divergence among Filamentous Vibriophages Mediating Lateral Gene Transfer ». Journal of Bacteriology 187, n^o 12 (15 juin 2005) : 4095–103. http://dx.doi.org/10.1128/jb.187.12.4095-4103.2005.

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ABSTRACT KSF-1Φ, a novel filamentous phage of Vibrio cholerae, supports morphogenesis of the RS1 satellite phage by heterologous DNA packaging and facilitates horizontal gene transfer. We analyzed the genomic sequence, morphology, and receptor for KSF-1Φ infection, as well as its phylogenetic relationships with other filamentous vibriophages. While strains carrying the mshA gene encoding mannose-sensitive hemagglutinin (MSHA) type IV pilus were susceptible to KSF-1Φ infection, naturally occurring MSHA-negative strains and an mshA deletion mutant were resistant. Furthermore, d-mannose as well as a monoclonal antibody against MSHA inhibited infection of MSHA-positive strains by the phage, suggesting that MSHA is the receptor for KSF-1Φ. The phage genome comprises 7,107 nucleotides, containing 14 open reading frames, 4 of which have predicted protein products homologous to those of other filamentous phages. Although the overall genetic organization of filamentous phages appears to be preserved in KSF-1Φ, the genomic sequence of the phage does not have a high level of identity with that of other filamentous phages and reveals a highly mosaic structure. Separate phylogenetic analysis of genomic sequences encoding putative replication proteins, receptor-binding proteins, and Zot-like proteins of 10 different filamentous vibriophages showed different results, suggesting that the evolution of these phages involved extensive horizontal exchange of genetic material. Filamentous phages which use type IV pili as receptors were found to belong to different branches. While one of these branches is represented by CTXΦ, which uses the toxin-coregulated pilus as its receptor, at least four evolutionarily diverged phages share a common receptor MSHA, and most of these phages mediate horizontal gene transfer. Since MSHA is present in a wide variety of V. cholerae strains and is presumed to express in the environment, diverse filamentous phages using this receptor are likely to contribute significantly to V. cholerae evolution.

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Chopin, Marie-Christine, Annette Rouault, S. Dusko Ehrlich et Michel Gautier. « Filamentous Phage Active on the Gram-Positive Bacterium Propionibacterium freudenreichii ». Journal of Bacteriology 184, n^o 7 (1 avril 2002) : 2030–33. http://dx.doi.org/10.1128/jb.184.7.2030-2033.2002.

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ABSTRACT We present the first description of a single-stranded DNA filamentous phage able to replicate in a gram-positive bacterium. Phage B5 infects Propionibacterium freudenreichii and has a genome consisting of 5,806 bases coding for 10 putative open reading frames. The organization of the genome is very similar to the organization of the genomes of filamentous phages active on gram-negative bacteria. The putative coat protein exhibits homology with the coat proteins of phages PH75 and Pf3 active on Thermus thermophilus and Pseudomonas aeruginosa, respectively. B5 is, therefore, evolutionarily related to the filamentous phages active on gram-negative bacteria.

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Campos, Javier, Eriel Martínez, Edith Suzarte, Boris L. Rodríguez, Karen Marrero, Yussuan Silva, Talena Ledón, Ricardo del Sol et Rafael Fando. « VGJφ, a Novel Filamentous Phage of Vibrio cholerae, Integrates into the Same Chromosomal Site as CTXφ ». Journal of Bacteriology 185, n^o 19 (1 octobre 2003) : 5685–96. http://dx.doi.org/10.1128/jb.185.19.5685-5696.2003.

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ABSTRACT We describe a novel filamentous phage, designated VGJφ, isolated from strain SG25-1 of Vibrio cholerae O139, which infects all O1 (classical and El Tor) and O139 strains tested. The sequence of the 7,542 nucleotides of the phage genome reveals that VGJφ has a distinctive region of 775 nucleotides and a conserved region with an overall genomic organization similar to that of previously characterized filamentous phages, such as CTXφ of V. cholerae and Ff phages of Escherichia coli. The conserved region carries 10 open reading frames (ORFs) coding for products homologous to previously reported peptides of other filamentous phages, and the distinctive region carries one ORF whose product is not homologous to any known peptide. VGJφ, like other filamentous phages, uses a type IV pilus to infect V. cholerae; in this case, the pilus is the mannose-sensitive hemagglutinin. VGJφ-infected V. cholerae overexpresses the product of one ORF of the phage (ORF112), which is similar to single-stranded DNA binding proteins of other filamentous phages. Once inside a cell, VGJφ is able to integrate its genome into the same chromosomal attB site as CTXφ, entering into a lysogenic state. Additionally, we found an attP structure in VGJφ, which is also conserved in several lysogenic filamentous phages from different bacterial hosts. Finally, since different filamentous phages seem to integrate into the bacterial dif locus by a general mechanism, we propose a model in which repeated integration events with different phages might have contributed to the evolution of the CTX chromosomal region in V. cholerae El Tor.

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Bulssico, Julián, Irina PapukashvilI, Leon Espinosa, Sylvain Gandon et Mireille Ansaldi. « Phage-antibiotic synergy : Cell filamentation is a key driver of successful phage predation ». PLOS Pathogens 19, n^o 9 (13 septembre 2023) : e1011602. http://dx.doi.org/10.1371/journal.ppat.1011602.

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Phages are promising tools to fight antibiotic-resistant bacteria, and as for now, phage therapy is essentially performed in combination with antibiotics. Interestingly, combined treatments including phages and a wide range of antibiotics lead to an increased bacterial killing, a phenomenon called phage-antibiotic synergy (PAS), suggesting that antibiotic-induced changes in bacterial physiology alter the dynamics of phage propagation. Using single-phage and single-cell techniques, each step of the lytic cycle of phage HK620 was studied in E. coli cultures treated with either ceftazidime, cephalexin or ciprofloxacin, three filamentation-inducing antibiotics. In the presence of sublethal doses of antibiotics, multiple stress tolerance and DNA repair pathways are triggered following activation of the SOS response. One of the most notable effects is the inhibition of bacterial division. As a result, a significant fraction of cells forms filaments that stop dividing but have higher rates of mutagenesis. Antibiotic-induced filaments become easy targets for phages due to their enlarged surface areas, as demonstrated by fluorescence microscopy and flow cytometry techniques. Adsorption, infection and lysis occur more often in filamentous cells compared to regular-sized bacteria. In addition, the reduction in bacterial numbers caused by impaired cell division may account for the faster elimination of bacteria during PAS. We developed a mathematical model to capture the interaction between sublethal doses of antibiotics and exposition to phages. This model shows that the induction of filamentation by sublethal doses of antibiotics can amplify the replication of phages and therefore yield PAS. We also use this model to study the consequences of PAS on the emergence of antibiotic resistance. A significant percentage of hyper-mutagenic filamentous bacteria are effectively killed by phages due to their increased susceptibility to infection. As a result, the addition of even a very low number of bacteriophages produced a strong reduction of the mutagenesis rate of the entire bacterial population. We confirm this prediction experimentally using reporters for bacterial DNA repair. Our work highlights the multiple benefits associated with the combination of sublethal doses of antibiotics with bacteriophages.

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Lin, Nien-Tsung, Tzu-Jun Liu, Tze-Ching Lee, Bih-Yuh You, Ming-Haw Yang, Fu-Shyan Wen et Yi-Hsiung Tseng. « The Adsorption Protein Genes of Xanthomonas campestris Filamentous Phages Determining Host Specificity ». Journal of Bacteriology 181, n^o 8 (15 avril 1999) : 2465–71. http://dx.doi.org/10.1128/jb.181.8.2465-2471.1999.

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ABSTRACT Gene III (gIII) of φLf, a filamentous phage specifically infecting Xanthomonas campestris pv. campestris, was previously shown to encode a virion-associated protein (pIII) required for phage adsorption. In this study, the transcription start site for the gene and the N-terminal sequence of the protein were determined, resulting in the revision of the translation initiation site from the one previously predicted for this gene. For comparative study, the gIII of φXv, a filamentous phage specifically infecting X. campestris pv. vesicatoria, was cloned and sequenced. The deduced amino acid sequences of these two pIIIs exhibit a high degree of identity in their C-terminal halves and possess the structural features typical of the adsorption proteins of filamentous phages: a signal sequence in the N terminus, a long glycine-rich region near the center, and a hydrophobic membrane anchorage domain in the C terminus. The regions between gIII and the upstreamgVIII, 128 nucleotides in both phages, are larger than those of other filamentous phages. A hybrid phage of φXv, consisting of the φLf pIII and all the other components derived from φXv, was able to infect X. campestris pv. campestris but notX. campestris pv. vesicatoria, indicating thatgIII is the gene specifying host specificity and demonstrating the interchangeability of the pIIIs.

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Ploss, Martin, et Andreas Kuhn. « Kinetics of filamentous phage assembly ». Physical Biology 7, n^o 4 (1 décembre 2010) : 045002. http://dx.doi.org/10.1088/1478-3975/7/4/045002.

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Nakasone, Noboru, Yasuko Honma, Claudia Toma, Tetsu Yamashiro et Masaaki Iwanaga. « Filamentous Phage fs1 ofVibrio choleraeO139 ». Microbiology and Immunology 42, n^o 3 (mars 1998) : 237–39. http://dx.doi.org/10.1111/j.1348-0421.1998.tb02277.x.

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Thèses sur le sujet "Filamentous phage"

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Weber, Patric. « Display of trypanosomal antigens on the surface of filamentous phage / ». [S.l.] : [s.n.], 1994. http://www.ub.unibe.ch/content/bibliotheken_sammlungen/sondersammlungen/dissen_bestellformular/index_ger.html.

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Lima, Mayara Ingrid Sousa. « Seleção e caracterização de peptídeos recombinantes do Mycobacterium leprae ligantes à IgG por meio da tecnologia de phage display ». reponame:Repositório Institucional da FIOCRUZ, 2011. https://www.arca.fiocruz.br/handle/icict/4253.

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Submitted by Ana Maria Fiscina Sampaio (fiscina@bahia.fiocruz.br) on 2012-07-30T21:26:19Z No. of bitstreams: 1 Mayara Ingrid Sousa Lima Seleção e caracterização de peptideos....pdf: 1915651 bytes, checksum: 4954d0969cc99ed5643d45ee27772173 (MD5)
Made available in DSpace on 2012-07-30T21:26:19Z (GMT). No. of bitstreams: 1 Mayara Ingrid Sousa Lima Seleção e caracterização de peptideos....pdf: 1915651 bytes, checksum: 4954d0969cc99ed5643d45ee27772173 (MD5) Previous issue date: 2011
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, Bahia,Brasil
A hanseníase é uma doença infecciosa crônica, causada pelo Mycobacterium leprae, que apresenta manifestações clínicas variadas. Essas variações refletem em diferenças que vão de uma forte resposta imune celular com controle do crescimento do bacilo, no pólo tuberculóide, a uma anergia em resposta celular, no pólo virchoviano. A caracterização do perfil antigênico do M. leprae frente a esse quadro de múltiplos aspectos clínicos representa uma ferramenta fundamental para o desenvolvimento de novas plataformas para um diagnóstico diferencial mais sensível e/ou desenvolvimento de unidades vacinais. Dessa forma, o objetivo desse trabalho foi selecionar e caracterizar peptídeos miméticos de antígenos do M. leprae reativos contra IgGs totais purificadas de pacientes com hanseníase. Para a seleção foi utilizada a tecnologia de phage display, usando bibliotecas randômicas de peptídeos expressos em fagos filamentosos. Foi realizada uma seleção com IgGs de pacientes Tuberculóides e outra com IgGs de pacientes Virchovianos. A validação dos peptídeos foi realizada utilizando o imunoensaio ELISA, o teste de redução de colônias e análise de bioinformática. Após a pré-validação e sequenciamento foram encontradas 17 mimotopos para o pólo Vichorviano e 12 no pólo Tuberculóide. Foram validados 4 peptídeos, sendo 2 do pólo Tuberculóide (T03, T04) e 2 do pólo Virchoviano (V06 e V13). Os peptídeos TALFPWL (T03) e YSTTLSY (T04) foram imunorreativos em soros de pacientes paucibacilares, bem como em pacientes Virchovianos, além de terem alinhado com proteínas de membrana do M. leprae com potencial antigênico. O peptídeo V06 apresentou especificidade de 100% e sensibilidade de 94,74%, o que se complementa com os dados do teste de redução da pIII, o qual obteve uma taxa de redução de 82% em soros Virchovianos. O peptídeo V13 também foi reativo e apresentou similaridades com chaperonas e proteínas de membrana. Este estudo aponta perspectivas para a identificação de novos antígenos, propiciando a descoberta de novos alvos biológicos com potencial diagnóstico e/ou terapêutico.
Leprosy is a chronic infectious disease caused by Mycobacterium leprae, which has varied clinical manifestations. These variations reflect differences that spans from a strong cellular mediated immunity and bacili growth control the tuberculoid pole to a poor T cell immunity at the lepromatous pole. The antigenic profile characterization in both clinical forms represents a fundamental tool for the development of new platforms for a differential diagnosis more sensitive and/or development of vaccine units. Thus, the objective was to select and characterize mimetics peptides antigens of M. leprae reactive against total IgG purified from leprosy patients. The phage display technology was used for selection using random peptides libraries expressed on filamentous phages. A selection was performed with IgGs from tuberculoid patients and other IgGs of lepromatous patients. Peptides validation was performed using the ELISA immunoassay, the plaque reduction test and bioinformatics analysis. After the pre-validation and sequencing were found 17 valid sequences for the lepromatous pole and 12 tuberculoid pole. Four peptides were validated, two of tuberculoid pole (T03, T04) and two lepromatous pole (V06 and V13). The peptides TALFPWL (T03) and YSTTLSY (T04) were imunoreatives in sera from paucibacillary patients and in lepromatous patients. They had alignment with membrane proteins of M. leprae antigenic potential. The V06 peptide showed 100% specificity and 94.74% sensitivity, which is supplemented with the plaque reduction test, who obtained a reduction rate of 82% in lepromatous sera. The V13 peptide was also reactive and showed similarities with chaperones and membrane proteins. This study presents insights for new antigens identification, leading to discovery of new biological targets with potential diagnostic or therapeutic

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Deng, L. W. « Infection mechanism of filamentous bacteriophage fd : interaction between E. coli F-pilus and phage protein pIII ». Thesis, University of Cambridge, 2000. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.598493.

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Gene 3 protein (pIII), a minor coat protein located at one end of the filamentous bacteriophage fd, is involved in initiating the infection by the virus of Escherichia coli cells that display an F-pilus. An introduction and the experimental materials and methods are described in Chapters 1 and 2, respectively. Chapter 3 describes the construction and expression of the N-terminal di-domain and individual domains of pIII protein. The plaque-forming assay in vivo was used to investigate the ability of the isolated pIII domains to interact with F-pilus. Chapter 4 presents the investigation of the structure of F pilus, including displaying fragments of F pilus subunit (pilin) by means of phage display technology, expression of pilin subunit and X-ray fibre-diffraction of F pilus. Site-directed alanine mutagenesis of the second N-terminal domain of pIII in the phage as well as the evaluation of their infectivity are described in Chapter 5. Chapter 6 describes the establishment of a competitive ELISA assay in vitro in order to analyse all the mutated phages. Combining the results from two assays and mapping out the affected residues on the 3D structure, a region located at the outer rim of pIII-D2 domain was implicated as the F pilus binding epitope. Second generation mutagenesis and evaluation of these mutants were performed in order to define the interface region more closely (Chapter 7). Furthermore, several pIII proteins each containing an alanine mutation were isolated to investigate whether any major disruption of protein integrity occurs. Finally, Chapter 8 places this work in perspective and offers ideas for further work.

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Mouville, Clémence. « Interaction entre les pili de type IV et le phage filamenteux MDA : impact potentiel sur la virulence de Neisseria meningitidis ». Electronic Thesis or Diss., Université Paris Cité, 2024. http://www.theses.fr/2024UNIP5235.

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Neisseria meningitidis (Nm) est une bactérie commensale du nasopharynx humain qui traverse quelques fois la barrière nasopharyngée et se propage dans la circulation sanguine jusqu'à atteindre les méninges. Un bactériophage filamenteux appelé MDA (Meningococcal Disease Associated) est associé aux infections invasives à méningocoques chez les jeunes adultes. Le MDA semble augmenter l'incidence de la maladie en augmentant la colonisation bactérienne au point d'entrée. L'objectif de ce travail a été de comprendre le mécanisme moléculaire précis de l'infection de Nm par le MDA. L'étude des mutants des gènes impliqués dans la machinerie des pili de type IV (PT4) a montré que l'entrée du phage nécessite un PT4 rétractable. Ce résultat est cohérent avec la littérature sur les phages Ff ou CTX qui interagissent directement avec l'extrémité du pilus. Mais aucune preuve de l'interaction du MDA avec l'extrémité du pilus n'a été trouvée. L'interaction possible entre la fibre du pilus et la capside du phage a été examinée. Comme PilE, la piline majeure, est sujette à variation antigénique, des variants de PilE qui diminuent l'entrée du phage ont été identifiés. L'infection par le phage se produit dans des populations de bactéries exprimant des séquences de PilE spécifiques. Par imagerie, nous avons montré que les pili et les MDA s'associaient ensemble. Une analyse de la charge des acides aminés de PilE et celle de la capside soutient l'hypothèse d'une interaction variable selon le variant de PilE. Finalement, il a été montré que les P4T avec un potentiel électrostatique positif favorisaient l'infection par le phage et permettaient, de plus, une forte adhésion des Nm aux cellules humaines. L'inverse est observé pour les P4T chargés négativement. Ce travail présente également les premières caractérisations de la machinerie de sécrétion du phage. Des études préliminaires avaient montré que dans un biofilm formé sur cellules épithéliales, Nm produisaient soit des pili, soit des phages, mais pas les deux en même temps. Nous avons montré que la sécrétion du phage nécessitait PilQ, PilW et TsaP de la machinerie des P4T. Les analyses bio-informatiques suggèrent que l'ORF8 du phage, qui aurait une activité ATPase, s'associerait avec l'ORF11 pour former un complexe qui interagirait avec la sécrétine bactérienne PilQ. Ce modèle est soutenu par des tests d'interaction par double-hybride. Cette interaction mobiliserait les PilQ de la bactérie, les rendant inaccessible à la machinerie de piliation. La surexpression d'ORF8 entraine une inhibition de la piliation. Ces données permettent d'établir un nouveau modèle d'interaction entre les phages filamenteux et les P4T, ce qui pourrait participer à la sélection des souches pathogènes de Nm
Neisseria meningitidis (Nm) is a commensal bacterium of the human nasopharynx that sometimes crosses the nasopharyngeal barrier and spreads through the bloodstream to reach the meninges. A filamentous bacteriophage called MDA (Meningococcal Disease Associated) is associated with invasive meningococcal disease in young adults. MDA appears to increase the incidence of the disease by increasing bacterial colonization at the point of entry. The aim of this work was to understand the precise molecular mechanism of infection of Nm by MDA. The study of mutants of genes involved in the type IV pili (T4P) machinery showed that phage entry requires a retractable T4P. This result is consistent with the literature on Ff or CTX phages, which interact directly with the pilus tip. However, no evidence was found for MDA interacting with the T4P tip. The possible interaction between the pilus fiber and the phage capsid was investigated. Since PilE, the major pilin, is subject to antigenic variation, variants of PilE that reduce phage entry were identified. Phage infection occurs in populations of bacteria that express specific PilE sequences. Using imaging, we showed that pili and MDA associate with each other. An analysis of the amino acid charge of the pilin and that of the capsid supports the hypothesis of a variable interaction depending on the PilE variant. Finally, it was shown that T4P with a positive electrostatic potential favored phage infection and allowed the bacteria to adhere strongly to human cells. The opposite is observed for negatively charged T4P. This work also presents the first characterizations of the phage secretion machinery. Previous studies had shown that in a biofilm formed on epithelial cells, Nm produce either pili or phages, but not both at the same time. We showed that phage secretion requires PilQ, PilW and TsaP of the pili machinery. Bioinformatic analyses suggest that phage ORF8, which has ATPase activity, associates with ORF11 to form a complex that interacts with the bacterial secretin PilQ. This model is supported by two-hybrid interaction assays. This interaction would mobilize the bacterial PilQ, making them inaccessible to the piliation machinery. Overexpression of ORF8 inhibits piliation. These data provide a new model for the interaction between filamentous phages and T4P, which could be involved in the selection of pathogenic strains of Nm

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Wang, Xiaoling. « Mechanical analysis and free energy construction of phase transition in bacterial flagellar filaments / ». View abstract or full-text, 2006. http://library.ust.hk/cgi/db/thesis.pl?MECH%202006%20WANGX.

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Rabiller, Philippe. « Etude et optimisation des propriétés supraconductrices de filaments à base de phase de Chevrel au plomb ». Rennes 1, 1991. http://www.theses.fr/1991REN10087.

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Ce memoire est consacre aux progres realises dans l'optimisation des filaments supraconducteurs a base de phase de chevrel au plomb pbmo#6s#8 (pms), progres qui peuvent etre resumes comme suit: 1) des traitements thermiques comprenant un palier a 750c pendant environ 24 heures suivi d'un recuit court a 1000c, ont permis d'obtenir pour la premiere fois des densites de courant critique elevees (>150 a/mm#2 a 20 tesla et 4,2 k) et surtout tres homogenes sur les fils dopes a l'etain. En effet pour des mesures sur des bobinettes de 12 spires de 30 mm de diametre, les variations de courant critique d'une spire a l'autre sont inferieures a 10%; 2) l'etude detaillee de la reactivite de la barriere anti-diffusion en niobium vis-a-vis de la poudre dans les fils a mis en evidence les criteres a respecter pour en minimiser les effets (diminution de la temperature de recuit et augmentation du taux de compactage). Ces criteres sont de plus en plus accessibles compte-tenu de l'amelioration des conditions de manipulation a l'abri de l'air et de compactage. En particulier, la temperature optimale de synthese in-situ dans les fils, a partir des precurseurs classiques, semble a present se situer autour de 900c; 3) la mise au point de nouvelles voies de synthese de melanges homogenes de precurseurs, mais aussi de la phase pms a basse temperature (<500c) a conduit a l'obtention de poudres tres fines dont la taille des grains est tres inferieure au micron. Des mesures de chaleur specifique ont revele que la phase pms ainsi obtenue est tres homogene, c'est-a-dire que le saut de chaleur specifique a la transition est tres etroit avec un debut de pic au-dela de 14 k

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Pommier, Stéphanie. « Le système Tol-Pal : l'intégrité membranaire et les interactions entre TolA, colicine A et G3p, protéine de capside des phages filamenteux ». Aix-Marseille 2, 2005. http://theses.univ-amu.fr.lama.univ-amu.fr/2005AIX22070.pdf.

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Le, Petitcorps Yann. « Caractérisations physico-chimique et mécanique de filaments CVD de carbure de silicium ou de bore : application aux matériaux composites 1D-Sci/Ti-6A1-4Av ». Bordeaux 1, 1985. http://www.theses.fr/1985BOR10592.

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Une caracterisation mecanique des filaments cvd de sic ou de b a ete effectuee en traction et interpretee sur la base de l'analyse de weibull. L'evolution des proprietes mecaniques des filaments apres des recuits sous vide a haute temperature (600-950c) en presence de titane a permis de definir de nouvelles conditions d'elaboration par compression a chaud de composites id-sic/ti-6al-4v. Des essais mecaniques et thermiques (traction, choc, cyclage thermique), ont mis en evidence les differents mecanismes de rupture. Cette etude souligne l'interet de deposer en surface des filaments, une couche de protection qui permet aux renforts et par voie de consequences aux composites d'avoir des proprietes mecaniques elevees et de les maintenir au cours de leur utilisation en temperature

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Le, Lay Luc. « Mise en forme et etude de filaments supraconducteurs a base de phases de chevrel ». Rennes 1, 1988. http://www.theses.fr/1988REN10126.

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Elaboration et etude de filaments pbmo::(6)s::(8) et snmo::(6)s::(8) et des massifs de ces memes materiaux. Etude des comportements mecanique, chimique et supraconducteur des massifs. Optimisation de la composition de la poudre d'un brin monofilamentaire en fonction des dopants, de la stoechiometrie et de la nature des poudres de base. Optimisation des traitements des filaments: des traitements a haute temperature (950**(o)c a 1000**(o)cpounds et pendant des durees relativement courtes (0,5 a 2 h) permettent d'obtenir les meilleurs resultats de densites de courant critique. Realisation de bobinettes permettant d'atteindre des densites de courant critique de 10**(8) a/m**(2) a 14 t

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Goislard, de Monsabert Thomas. « Couches de nanotubes et filaments de carbone pour l'émission froide d'électrons : intégration aux écrans plats à émission de champ ». Université Joseph Fourier (Grenoble), 2006. http://www.theses.fr/2006GRE10043.

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Ce travail concerne l'élaboration in situ, par CVD catalytique, de couches de nanotubes et filaments de carbone pour leur intégration en tant que couches émissives d'électrons dans les écrans plats à émission de champ. Les paramètres clés, avantages et limitations de plusieurs techniques de préparation et d'intégration de nano particules catalytiques ont d'abord été analysés : le démouillage d'un film continu, la gravure humide post-démouillage, le dépôt de nano agrégats et la lithographie électronique. Trois techniques de croissance de couches carbonées ont ensuite été étudiées dans le même réacteur : la CVD thermique simple, la CVD en présence d'un champ électrique et la CVD avec assistance plasma à partir d'une source de carbone solide. Enfin, les propriétés émissives des diverses couches carbonées élaborées ont été mesurées, en mode diode pour les couches synthétisées sur échantillon plan et en mode triode pour les couches intégrées sur structure cathodique d'écran. L'analyse de ces résultats a permis de clarifier les liens entre paramètres technologiques d'élaboration, morphologie et performances émissives des films de nanotubes et filaments de carbone
This work concerns the in situ elaboration, by catalytic CVD, of carbon nanotubes and filaments films for their integration as electron emissive films into field emission displays. The key parameters, advantages and restrictions of several techniques for catalyst nano particles preparation and integration were first analysed : dewetting of a continuous layer, post-dewetting wet etching, nano cluster deposition and e-beam lithography. Three growth techniques were then studied in the same reactor : simple thermal CVD, CVD with an electrical field and plasma assisted CVD from a solid carbon source. Finally, the emissive properties of the elaborated carbonaceous films were measured, in diode mode for the full sheet type samples and in triode mode for the films integrated into display cathodic structures. Theses results analysis permitted to clarify the links between technological elaboration parameters, morphology and emissive performances of carbon nanotubes and filaments films

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Livres sur le sujet "Filamentous phage"

1

Roberts, Linda Marie. Characterization of the chloroform/water interface-induced contraction of the filamentous phage fd. 1990.

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Chapitres de livres sur le sujet "Filamentous phage"

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Rakonjac, Jasna, Marjorie Russel, Sofia Khanum, Sam J. Brooke et Marina Rajič. « Filamentous Phage : Structure and Biology ». Dans Recombinant Antibodies for Infectious Diseases, 1–20. Cham : Springer International Publishing, 2017. http://dx.doi.org/10.1007/978-3-319-72077-7_1.

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Kulseth, Mari Ann, Annette Fagerlund et Astrid Hilde Myrset. « Affinity Selection Using Filamentous Phage Display ». Dans Methods in Molecular Biology, 67–80. Totowa, NJ : Humana Press, 2013. http://dx.doi.org/10.1007/978-1-62703-673-3_5.

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Fagerlund, Annette, Astrid Hilde Myrset et Mari Ann Kulseth. « Construction of a Filamentous Phage Display Peptide Library ». Dans Methods in Molecular Biology, 19–33. Totowa, NJ : Humana Press, 2013. http://dx.doi.org/10.1007/978-1-62703-673-3_2.

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Cao, Binrui, et Chuanbin Mao. « Chapter 10. Filamentous Phage-templated Synthesis and Assembly of Inorganic Nanomaterials ». Dans Nanoscience & ; Nanotechnology Series, 220–44. Cambridge : Royal Society of Chemistry, 2011. http://dx.doi.org/10.1039/9781847559920-00220.

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Duché, Denis, et Laetitia Houot. « Similarities and Differences between Colicin and Filamentous Phage Uptake by Bacterial Cells ». Dans Protein Secretion in Bacteria, 375–87. Washington, DC, USA : ASM Press, 2019. http://dx.doi.org/10.1128/9781683670285.ch30.

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Parmley, Stephen F., et George P. Smith. « Filamentous Fusion Phage Cloning Vectors for the Study of Epitopes and Design of Vaccines ». Dans Immunobiology of Proteins and Peptides V, 215–18. Boston, MA : Springer US, 1989. http://dx.doi.org/10.1007/978-1-4757-2046-4_21.

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Tasaka, Yuichi, Takeru Kawasaki et Takashi Yamada. « Filamentous Phages Affect Virulence of the Phytopathogen Ralstonia solanacearum ». Dans Biocommunication of Phages, 221–37. Cham : Springer International Publishing, 2020. http://dx.doi.org/10.1007/978-3-030-45885-0_11.

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Ehara, Masahiko, et M. John Albert. « Filamentous Phages of Vibrio cholerae O1 and O139 ». Dans Epidemiological and Molecular Aspects on Cholera, 213–21. New York, NY : Springer New York, 2010. http://dx.doi.org/10.1007/978-1-60327-265-0_12.

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Müller, Henri, Stefan Schmideder et Heiko Briesen. « Generalized Morphology Modeling of Aggregating, Filamentous Microorganisms ». Dans Dispersity, Structure and Phase Changes of Proteins and Bio Agglomerates in Biotechnological Processes, 441–65. Cham : Springer Nature Switzerland, 2024. http://dx.doi.org/10.1007/978-3-031-63164-1_14.

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Hüttinger, K. J. « Theoretical and Practical Aspects of Liquid-Phase Pyrolysis as Basis of the Carbon Matrix of CFRC ». Dans Carbon Fibers Filaments and Composites, 301–25. Dordrecht : Springer Netherlands, 1990. http://dx.doi.org/10.1007/978-94-015-6847-0_13.

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Actes de conférences sur le sujet "Filamentous phage"

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Sydney, Anish, et J. Leishman. « Measurements of the Plume-like Three-Dimensionality of Rotor-Induced Dust Fields ». Dans Vertical Flight Society 70th Annual Forum & Technology Display, 1–20. The Vertical Flight Society, 2014. http://dx.doi.org/10.4050/f-0070-2014-9436.

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To better understand the problem of rotor-induced particle motion and rotorcraft brownout, time-resolved, dual-phase particle image velocimetry and particle tracking velocimetry measurements were made in the flow produced by a small laboratory rotor that was hovering over a mobile sediment bed. The main goal was to further investigate the three-dimensionality of the rotor wake and how its interaction with the sediment bed led to the formation of distinct columnar plumes of uplifted sediment particles. Dual-phase flow measurements were made in both vertical and horizontal planes around the rotor and near the ground. The results showed that the rotor wake was extremely three-dimensional, with non-uniform velocities near the ground that resulted in the radially asymmetric uplift of particles throughout the flow field. The helicoidal vortex filament developed wave-like displacements along their lengths, which caused some parts of the filaments to convect closer to the ground. The parts of the filaments that were closer to the ground were observed to interact with the near-wall flow there, sometimes causing the boundary layer to briefly detach and introduce secondary vorticity into the flow. Adjacent turns of the interdigitated helicoidal vortex filaments often merged together, creating significantly larger upwash/downwash regions near the ground, which produced discrete bursts of sediment mobilization and uplift.

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Kashiwagi, Kenji, et Kiyotaka Shiba. « Filamentous Phage-Based Extra Cellular Matrix ». Dans 2008 International Symposium on Micro-NanoMechatronics and Human Science (MHS). IEEE, 2008. http://dx.doi.org/10.1109/mhs.2008.4752484.

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Burgener, Elizabeth, Laura Rojas Hernandez, Paul Bollyky et Carlos Milla. « The filamentous Pseudomonas phage Pf disrupts airway basal cell proliferation ». Dans ERS International Congress 2021 abstracts. European Respiratory Society, 2021. http://dx.doi.org/10.1183/13993003.congress-2021.pa3616.

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Petrov, G. O., V. S. Mukhanov et M. A. Dymova. « DEVELOPMENT M13 BACTERIOPHAGE-BASED TARGETED HYBRID VECTORS FOR HUMAN GLIOBLASTOMA CELL SELECTIVE TRANSDUCTION ». Dans X Международная конференция молодых ученых : биоинформатиков, биотехнологов, биофизиков, вирусологов и молекулярных биологов — 2023. Novosibirsk State University, 2023. http://dx.doi.org/10.25205/978-5-4437-1526-1-357.

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Bacteriophages, bacterial viruses, are currently being used as source of effective gene therapy tools for cancer. The aim of this study was to develop targeted hybrid vectors based on filamentous phage M13, capable of efficient and selective delivery of transgenes into U-87 MG human glioblastoma cells.

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Salzman, J., A. Larsson et A. Yariv. « Phase-locked laser array formed by induced filaments ». Dans OSA Annual Meeting. Washington, D.C. : Optica Publishing Group, 1985. http://dx.doi.org/10.1364/oam.1985.tui2.

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The necessary condition for a uniform index-guided phase-locked laser array to operate in the fundamental supermode is to have high gain in the regions of low refractive index and vice versa. This condition is fulfilled by a self-focused laser filament. Therefore, if filamentation could be controlled to form stripes uniformly spaced and phase locked, the expected emission would be in the fundamental supermode. Filamentation control was achieved experimentally by etching one of the mirrors of a broad area laser in a sinusoidal form. The alternating converging and diverging regions of the mirror efficiently stabilized the filament array. Since the device relies on self-focusing, it is sensitive neither to the laser length nor to the exact shape of the etched mirror. We observed emission in the fundamental supermode, with a stable near-field intensity distribution. Devices fabricated so that the filament spacing was comparable to the filament width emitted in a single lobe, nearly diffraction-limited far field. In addition to the potential applications, the device provides a powerful tool for studying self-focusing due to the nonlinear response in semiconductor lasers. As an illustration of that, measurements of the filament width and phase front curvature as a function of injection current are presented.

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Fisher, M., T. Saunders, M. Bublenik, E. Johnson et C. Siders. « Control and manipulation of femtosecond laser filaments by the use of vortex phase elements ». Dans 2005 Conference on Lasers and Electro-Optics (CLEO). IEEE, 2005. http://dx.doi.org/10.1109/cleo.2005.201722.

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Pajic, Tanja, Natasa Todorovic, Dunja Stefanovic, Mihailo Rabasovic, Aleksandar Krmpot et Miroslav Zivic. « THE EFFECTS OF SELENITE ON FILAMENTOUS FUNGI LIPID DROPLETS MONITORED „IN VIVO“ LABEL FREE USING ADVANCED NONLINEAR MICROSCOPY TECHNIQUE 2021ICCBIKG (2021) ». Dans 1st INTERNATIONAL Conference on Chemo and BioInformatics. Institute for Information Technologies, University of Kragujevac, 2021. http://dx.doi.org/10.46793/iccbi21.300p.

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Third Harmonic Generation (THG) microscopy was employed as a method of choice for lipid droplet (LD) measurements and quantification of the effect of selenite on LDs. Nonlinear laser scanning microscopy (NLSM) employs ultra-short laser pulses for imaging. THG microscopy is the modality of NLSM. Strong THG signals can only be observed from regions with non- uniformities with respect to their refractive index. Such regions in biological samples are lipid-water interfaces, and by far the brightest features in cells are LDs. For that reason, THG microscopy is the appropriate method for imaging of LDs from live unfixed cells, without the need for additional labeling. The biological effects of spore- to- end- of- exponential- phase duration (27 – 30 h) of exposure to 1 mM selenite were monitored in vivo on the cells of filamentous fungi in liquid culture. We measured the lipid droplet density and size distribution in a model fungi Phycomyces blakesleeanus. The in-house built microscope frame complemented with Yb KGW laser (1040 nm, 200 fs pulses) was used, while detection was enabled in the transmission arm by PMT through the Hoya glass UV filter (peak at 340 nm). From THG images of control and Se+4–treated hyphae, LD size and number were measured, showing that LD density was increased by more than 60% in Se+4–treated hyphae, compared to control. The average LD size distribution seemed slightly changed by Se+4 -treatment. The obtained results suggest that 1 mM selenite treatment probably induces cellular stress response in filamentous fungi.

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Lamb, Robert A., Kevin J. Cook et Ajoy K. Kar. « Phase stability and diffraction effects in self-focused white-light filaments in water and glass ». Dans European Symposium on Optics and Photonics for Defence and Security, sous la direction de Jonathan A. C. Terry et W. Andrew Clarkson. SPIE, 2004. http://dx.doi.org/10.1117/12.578075.

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Oliveira, Gonçalo, Bernardo Alves, Ricardo Mineiro, Ana Maria Rocha Senos, Cristina Fernandes, Daniel Figueiredo et Maria Teresa Vieira. « Indirect Additive Manufacturing (Material Extrusion) as a Solution to a New Concept of Cutting Tools ». Dans World Powder Metallurgy 2022 Congress & Exhibition. EPMA, 2022. http://dx.doi.org/10.59499/wp225366866.

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A cermet grade with TiCN as major phase and 15wt.% Co/Ni as the binder and secondary carbides WC, Mo2C and NbC was selected for indirect additive manufacturing (Material Extrusion). These powder constituents were the primary material of feedstocks to produce filaments for the indirect AM process - Material Extrusion (MEX). The filaments result from the extrusion of a feedstock previously optimized (CPVC= critical powder volume concentration) and selection of polymeric binder and additive. Concerning the cermet powder particles, 4Ss (particle size, particle size distribution, particle shape, and particle structure) and the quality of the organic binder/additives through the feedstock and filament behaviour were evaluated, in what concerns "printability" and sinterability. Cermet 3D-objects were successfully printed by MEX and sintered. No significant deformation was measured after debinding and sintering, and no undesired phases were detected in the microstructures of the 3D-object.

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Derstine, M. W., H. M. Gibbs, F. A. Hopf et J. F. Valley. « Dual-Phase Oscillation from CW Transverse Instabilities in Sodium Vapor ». Dans Optical Bistability. Washington, D.C. : Optica Publishing Group, 1985. http://dx.doi.org/10.1364/obi.1985.md17.

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Recent experiments1 on transverse instabilities in Gaussian laser beams using sodium vapor for the nonlinear medium have shown well-behaved oscillations of the type first proposed by Ikeda.2 The period of such oscillations for a medium with infinitely fast response is given by 2τr, where τR is the time it takes for light to traverse the feedback loop. Our experimental arrangement (Fig. 1) uses a ring dye laser with well-defined transverse properties tuned to a frequency 2 to 10 GHz below the sodium D2 resonance. Operation on the self-defocusing side guarantees that no self-trapped filaments are formed. A feedback mirror several meters from the sodium sample provides the delay needed for the Ikeda instability.

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Rapports d'organisations sur le sujet "Filamentous phage"

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Weisgraber, T. Computed Stress Distributions Within DIW Filaments Containing Particulate Phase. Office of Scientific and Technical Information (OSTI), septembre 2021. http://dx.doi.org/10.2172/1821268.

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Benemann, John. Final Report for Phase 1 SBIR : “Power Plant CO₂ Capture in Filamentous Algae for Animal Feeds”. Office of Scientific and Technical Information (OSTI), octobre 2018. http://dx.doi.org/10.2172/1476637.

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Dickman, Martin B., et Oded Yarden. Role of Phosphorylation in Fungal Spore Germination. United States Department of Agriculture, août 1993. http://dx.doi.org/10.32747/1993.7568761.bard.

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Spore germination is a common and fundamental event in fungal development and in many instances an essential phase of fungal infection and dissemination. Spore germination is also critical for hyperparasites to function as biocontrol agents as well as in fermentation proceses. Our common objective is to understand the mechanisms which regulated spore germination and identify factors involved in pathogenicity related prepenetration development. Our approach is to exploit the overall similarity among filamentous fungi using both a plant pathogen (Colletotricum trifolii) and a model system that is genetically sophisticated (Neurospora crassa). The simulataneous use of two organisms has the advantage of the available tools in Neurospora to rapidly advance the functional analysis of genes involved in spore germination and development of an economically important fungal phytopathogen. Towards this we have isolated a protein kinase gene from C. trifolii (TB3) that is maximally expressed during the first hour of conidial germination and prior to any visible gene tube formation. Based on sequence similarities with other organisms, this gene is likely to be involved in the proliferative response in the fungus. In addition, TB3 was able to functionally complement a N. crassa mutant (COT-1). Pharmacological studies indicated the importance of calmodulin in both germination and appressorium differentiation. Using an antisense vector from N. crassa, direct inhibition of calmodulin results in prevention of differentiation as well as pathogenicity. Both cAMP dependent protein kinase (PKA) and protein kinase C (PKC) like genes have been cloned from C. trifolii. Biochemical inhibition of PKA prevents germination; biochemical inhibitors of PKC prevents appressorium differentiation. In order to analyze reversible phosphorylation as a regulatory mechanism, some ser.thr dephosphorylative events have also been analyzed. Type 2A and Type 2B (calcineurin) phosphatases have been identified and structurally and functionally analyzed in N. crassa during this project. Both phosphatases are essential for hyphal growth and maintenance of proper hyphal architecture. In addition, a first novel-type (PPT/PP5-like) ser/thr phosphatase has been identified in a filamentous fungus. The highly collaborative project has improved our understanding of a fundamental process in fungi, and has identified targets which can be used to develop new approaches for control of fungal plant pathogens as well as improve the performance of beneficial fungi in the field and in industry. In addition, the feasibility of molecular technology transfer in comparative mycology has been demonstrated.

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