Thèses sur le sujet « Flow cell »
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Rabodzey, Aleksandr. « Flow-induced mechanotransduction in cell-cell junctions of endothelial cells ». Thesis, Massachusetts Institute of Technology, 2006. http://hdl.handle.net/1721.1/41586.
Texte intégralIncludes bibliographical references (leaves 86-92).
Endothelial cells show an unexpected behavior shortly after the onset of laminar flow: their crawling speed decreases ~40% within the first 30 min, but only in a confluent monolayer of endothelial cells, not in subconfluent cultures, where cell-cell interactions are limited. This led us to study early shear effects on cell-cell adherens junctions. We found a 30±6% increase in the number of VE-cadherin molecules in the junctions. The strength of interactions of endothelial cells with surfaces coated with recombinant VE-cadherin protein also increased after laminar flow. These observations suggest that endothelial cell junction proteins respond to flow onset. The process of clustering may induce diffusion of monomers to the junction area, resulting in an overall increase in VE-cadherins in the junctions. To directly confirm the role of adherens junctions in the decrease in cell crawling speed, we used siRNA-knockdown technique to produce cells lacking VE-cadherin. These cells showed no decline in crawling speed under flow. Our interpretation is consistent with previous data on junction disassembly 8 hr after flow onset. The speed of endothelial cell crawling returns to the original level by that time, and junctional disassembly may explain that phenomenon. In order to understand better the change in VE-cadherin distribution under flow and during junction formation and remodelling, we developed a mathematical model of VE-cadherin redistribution in endothelial cells. This model allowed us to develop a quantitative framework for analysis of VE-cadherin redistribution and estimate the amount of protein in the junctions and on the apical surface. In addition to that, the model explains rapid junction disassembly in the leukocyte transmigration and junction formation in subconfluent cells.
(cont.) These studies show that intercellular adhesion molecules are important in the force transmission and shear stress response. Their role, however, is not limited to flow mechanotransduction. Intercellular force transmission has an important application - organ development and, specifically, angiogenesis. We studied the role of VE-cadherin in vessel development in HUVECs and showed that VE-cadherin-null cells do not form vessels in the in vitro assay. This observation confirms the important role of intercellular force transmission in response to external force caused by flow or exerted by other cells.
by Aleksandr Rabodzey.
Ph.D.
Kucukal, Erdem. « BIOMIMETIC MICROFLUIDIC PLATFORMS FOR MONITORING CELLULAR INTERACTIONS IN MICROSCALE FLOW ». Case Western Reserve University School of Graduate Studies / OhioLINK, 2020. http://rave.ohiolink.edu/etdc/view?acc_num=case1576231265150031.
Texte intégralOfsthun, Norma Jean. « Cross-flow membrane filtration of cell suspensions ». Thesis, Massachusetts Institute of Technology, 1989. http://hdl.handle.net/1721.1/14481.
Texte intégralHan, Tian. « Flow cell separation in fluctuating g-field ». Thesis, Brunel University, 2015. http://bura.brunel.ac.uk/handle/2438/11105.
Texte intégralChoe, Juno. « Genomic analysis by single cell flow sorting / ». Thesis, Connect to this title online ; UW restricted, 2003. http://hdl.handle.net/1773/10850.
Texte intégralDive, C. « Flow cytoenzymology with special reference to cancer chemotherapy ». Thesis, Open University, 1988. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.384585.
Texte intégralCamplejohn, Richard Stephen. « Cell kinetics and cancer ». Thesis, University of Newcastle Upon Tyne, 2000. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.327272.
Texte intégralKorn, Christian. « Stochastic dynamics of cell adhesion in hydrodynamic flow ». Phd thesis, Universität Potsdam, 2007. http://opus.kobv.de/ubp/volltexte/2007/1299/.
Texte intégralAllen, R. J. « Modelling the endothelial cell response to fluid flow ». Thesis, University College London (University of London), 2009. http://discovery.ucl.ac.uk/16119/.
Texte intégralPolacheck, William J. (William Joseph). « Effects of interstitial flow on tumor cell migration ». Thesis, Massachusetts Institute of Technology, 2010. http://hdl.handle.net/1721.1/61917.
Texte intégralCataloged from PDF version of thesis.
Includes bibliographical references (p. 80-84).
Interstitial flow is the convective transport of fluid through tissue extracellular matrix. This creeping fluid flow has been shown to affect the morphology and migration of cells such as fibroblasts, cancer cells, endothelial cells, and mesenchymal stem cells. However, due to limitations in experimental procedures and apparatuses, the mechanism by which cells detect flow and the details and dynamics of the cellular response remain largely unknown. We developed a microfluidic cell culture system in which we can apply stable pressure gradients and fluid flow, and in which we can observe transient responses of breast cancer cells seeded in a 3D collagen type I scaffold. We employed this system to examine cell migration in the presence of interstitial flow to address the hypothesis that interstitial flow increases the metastatic potential of breast cancer cells. By varying the concentration of chemoattractants, we decoupled the mechanisms that provide the migratory stimulus and the directional stimulus to migrating breast cancer cells in the presence of a flow field. We found that cells migrated along streamlines in the presence of flow and that the strength of the flow field determined directional bias of migration along the streamline. We provide evidence that CCR7-dependent autologous chemotaxis is the mechanism by which cells migrate with the flow, while a competing CCR7-independent mechanism leads to migration against the flow. Furthermore, we demonstrate these competing mechanisms are a powerful migrational stimulus, which likely play an important role in development of metastatic disease.
by William J. Polacheck.
S.M.
Bose, Suman. « Affinity Flow Fractionation for label-free cell sorting ». Thesis, Massachusetts Institute of Technology, 2014. http://hdl.handle.net/1721.1/87961.
Texte intégralCataloged from PDF version of thesis.
Includes bibliographical references (pages [107]-118).
Capture and isolation of flowing cells from body fluids such as peripheral blood, bone marrow or pleural effusion has enormous implications in diagnosis, disease monitoring, and drug testing. However, in many situations the conventional methods of cell sorting are of limited use due to complex sample preparation steps, high costs, or low sensitivity. Drawing inspiration from nature, a novel platform technology for cell separation known as Affinity Flow Fractionation (AFF) was developed. AFF relies on interaction of cells with asymmetric patterns of weak adhesive molecules allowing for continuous sorting of cells with high purity without irreversible capture of cells. Cells are sorted in a single step, which is a significant advance over conventional immunocapture methods, especially for point-of-care and point-of-use applications. In this work, first, the interaction of cells under shear flow with asymmetric patterns of weak adhesive molecules was studied systematically to highlight the underlying mechanism of AFF at a phenomenological level. Next, an optimized separation device was fabricated and its performance was characterized using model cell lines. A detailed predictive mathematical model, which accounts for the major transport processes involved in cell separation by AFF, was developed and the results validated using experiments. Finally, AFF was applied for rapid isolation of neutrophils from blood, which is important for several applications where conventional procedures involve multiple steps and time-intense manual skills. It was demonstrated that asymmetric patterns of Pselectin, a weak adhesive molecule involved in cell trafficking, can directly draw neutrophils out of a continuously flowing stream of blood, with high purity (92%). As cells exhibiting non-specific adhesion are not drawn out of the flowing stream, an ultrahigh 400,000-fold enrichment of leukocytes over erythrocytes is achieved. Moreover, the sorted neutrophils remain viable, unaltered, and functionally intact. The lack of background erythrocytes enabled direct enumeration of neutrophils by a downstream detector, which could distinguish the activation state of neutrophils in blood. This method is compatible with capillary microfluidics and may find use in isolation of neutrophils for diagnosis of sepsis, genetic analysis, HLA typing, assessment of chemoreadiness, and other applications. Weak molecular interactions govern a large number of important physiological processes such as stem cell homing, inflammation, immune modulation and cancer metastasis. Yet, currently there are no effective technologies that can separate cells based on weak interactions alone. We believe, AFF would fulfill this un-met need in the area of cell sorting and enabling new discoveries. Keywords: Microfluidics, Cell sorting, cell rolling, selectin, blood, point-of-care, neutrophils.
by Suman Bose.
Ph. D.
Kimpton, Laura Saranne. « On two-phase flow models for cell motility ». Thesis, University of Oxford, 2013. http://ora.ox.ac.uk/objects/uuid:93c3cc12-4aac-424d-83bf-3e695efb49fe.
Texte intégralBrown, Jason Britton. « An experimental facility for the investigation of the flow in a circular-couette flow bioreactor ». Thesis, Georgia Institute of Technology, 1998. http://hdl.handle.net/1853/18174.
Texte intégralLähdesmäki, Ilkka Johannes. « Flow injection methods for drug-receptor interaction studies, based on probing cell metabolism / ». Thesis, Connect to this title online ; UW restricted, 1999. http://hdl.handle.net/1773/8590.
Texte intégralSilva, Michael Santos. « Flow cytometric of c-FLIPl-mediated regulation of cell cycle and cell population size ». Master's thesis, Universidade de Aveiro, 2017. http://hdl.handle.net/10773/22487.
Texte intégralc-FLIP é uma proteína conhecida pela sua capacidade de se ligar ao DISC, onde compete com a procaspase-8 pela interação com FADD. No entanto, existem evidências que a sua isoforma longa consegue regular também o ciclo celular e mecanismos de proliferação. Para além disso, a atividade de c-FLIPL pode ser controlada por fosforilação. Assim, o objetivo deste estudo é perceber como a fosforilação no resíduo de serina 227 nesta proteína afeta a proliferação e ciclo celular. Neste estudo, observamos que a sobre-expressão de c-FLIPL com uma mutação de serina para alanina no resíduo 227 levou a uma diminuição da capacidade proliferativa dessas células. O uso de citometria de fluxo permitiu verificar este decréscimo na capacidade proliferativa, assim como uma acumulação de células na fase G1 do ciclo celular aquando da sobre-expressão de S227A c-FLIPL. Os resultados obtidos sugerem que a sobre-expressão de c-FLIPL controla a população celular através da transição G1/S, através da sua fosforilação no resíduo 227. No entanto, mais estudos são necessários para se perceber a partir de qual mecanismo esta transição é afetada
c-FLIP is a protein known for its capacity to bind to the DISC and compete with procaspase-8 for FADD interaction. However, published studies have shown that c-FLIPL can regulate cell cycle and proliferation. Similarly to many other proteins, c-FLIP can be regulated by phosphorylation. Therefore, the aim of this work was to understand how the phosphorylation of S227 residue on c-FLIPL affects cell cycle and cell proliferation. We observed that overexpression of phosphodeficient mutant c-FLIPL lead to a decrease in cell proliferation. Flow cytometric analysis confirmed this decrease, as well as an accumulation of cell at G1 phase of cell cycle, when overexpressing S227A c-FLIPL. Our results suggest that c-FLIPL overexpression controls cell population size by controlling the G1/S transition, via its phosphorylation. Nonetheless, further studies need to be done to understand which mechanism affects this transition.
Shaffer, Christian Edward. « Flow system modeling with applications to fuel cell systems ». Morgantown, W. Va. : [West Virginia University Libraries], 2005. https://eidr.wvu.edu/etd/documentdata.eTD?documentid=4198.
Texte intégralTitle from document title page. Document formatted into pages; contains xii, 111 p. : ill. (some col.). Includes abstract. Includes bibliographical references (p. 100-102).
Tamaresis, John S. « Mathematical modeling of arterial endothelial cell responsiveness to flow / ». For electronic version search Digital dissertations database. Restricted to UC campuses. Access is free to UC campus dissertations, 2004. http://uclibs.org/PID/11984.
Texte intégralRaina, Rajeev. « Development of a cell-based stream flow routing model ». Thesis, Texas A&M University, 2003. http://hdl.handle.net/1969.1/2219.
Texte intégralHasson, Ali Thuriya A. Razzak. « A flow cell to study formation potential of pulps ». Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1997. http://www.collectionscanada.ca/obj/s4/f2/dsk2/ftp03/MQ29377.pdf.
Texte intégralYamamoto, Miharu. « Coupling of Arterial Wall Cell Dynamics and Blood Flow ». Thesis, University of Canterbury. Centre for Bioengineering, 2011. http://hdl.handle.net/10092/6288.
Texte intégralAlexander, R. G. « Flow cytometry and cell sorting in plant genetic manipulations ». Thesis, University of Nottingham, 1986. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.356016.
Texte intégralSayer, Robert Michael. « Atir flow cell developments and studies in atmospheric chemistry ». Thesis, University of York, 2001. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.250623.
Texte intégralMadden, Jacqueline. « Flow cytometric assessment of T cell activation in asthma ». Thesis, University of Southampton, 1998. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.245048.
Texte intégralBremner, Sherry. « A granular flow model of an annular shear cell ». Doctoral thesis, University of Cape Town, 2016. http://hdl.handle.net/11427/20304.
Texte intégralReyes, Eduardo Alberto. « Flow chamber for studying cell attachment to opaque substrates ». [Gainesville, Fla.] : University of Florida, 2004. http://purl.fcla.edu/fcla/etd/UFE0008971.
Texte intégralMelane, Xolani. « Visualisation of electrolyte flow fields in an electrolysis cell ». Diss., University of Pretoria, 2015. http://hdl.handle.net/2263/57492.
Texte intégralDissertation (MEng)--University of Pretoria, 2015.
tm2016
Chemical Engineering
MEng
Unrestricted
McIlvenna, David. « Raman activated cell sorting and counting in continuous flow ». Thesis, University of Glasgow, 2015. http://theses.gla.ac.uk/6333/.
Texte intégralDuqi, Enri. « Continuos Flow Single Cell Separation into Open Microwell Arrays ». Doctoral thesis, Alma Mater Studiorum - Università di Bologna, 2012. http://amsdottorato.unibo.it/4778/1/duqi_enri_tesi.pdf.
Texte intégralDuqi, Enri. « Continuos Flow Single Cell Separation into Open Microwell Arrays ». Doctoral thesis, Alma Mater Studiorum - Università di Bologna, 2012. http://amsdottorato.unibo.it/4778/.
Texte intégralPoles, Richard R. « Free-surface flow in a shallow laterally heated cavity ». Thesis, City University London, 1997. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.363313.
Texte intégralPinto, Preston Albert. « Novel Bio-inspired Aquatic Flow Sensors ». Thesis, Virginia Tech, 2012. http://hdl.handle.net/10919/33807.
Texte intégralMaster of Science
Zheng, Hong. « Efficient Algorithms for the Cell Based Single Destination System Optimal Dynamic Traffic Assignment Problem ». Diss., The University of Arizona, 2009. http://hdl.handle.net/10150/195304.
Texte intégralChu, Chun-sing, et 朱振聲. « Flow cytometric analysis of cell-cycle regulatory proteins during apoptosis ». Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2000. http://hub.hku.hk/bib/B31240070.
Texte intégralChu, Chun-sing. « Flow cytometric analysis of cell-cycle regulatory proteins during apoptosis / ». Hong Kong : University of Hong Kong, 2000. http://sunzi.lib.hku.hk/hkuto/record.jsp?B21490144.
Texte intégralTrotter, Martin James. « Intermittent blood flow in the murine SCCVII squamous cell carcinoma ». Thesis, University of British Columbia, 1990. http://hdl.handle.net/2429/31344.
Texte intégralMedicine, Faculty of
Pathology and Laboratory Medicine, Department of
Graduate
Martin, Ashley Diane. « Modulation of endothelial cell characteristics by pericytes ». Thesis, University of Ulster, 1998. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.287133.
Texte intégralPiri, Hossein. « Flow visualization in 3D printed PEM fuel cell bipolar plates ». Thesis, University of British Columbia, 2017. http://hdl.handle.net/2429/62259.
Texte intégralApplied Science, Faculty of
Chemical and Biological Engineering, Department of
Graduate
Godoy, Ramirez Karina. « Flow cytometric methods for assessment of cell-mediated immune responses / ». Stockholm, 2005. http://diss.kib.ki.se/2005/91-7140-409-0/.
Texte intégralO'Connell, Gregory Raymond. « Detector cell hydrodynamics and electrode selectivity in flow-injection potentiometry ». Thesis, Brunel University, 1990. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.290935.
Texte intégralBae, C. H. « Cell design and electrolytes of a Novel Redox flow battery ». Thesis, University of Manchester, 2001. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.509374.
Texte intégralBessaih, Nabil. « Erosion of sediment from a cell under high velocity flow ». Thesis, University of Salford, 1994. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.386404.
Texte intégralMedina, Jose L. « Work-cell Centric Satellite Pulse Flow Production System Integration Test ». Digital Commons at Loyola Marymount University and Loyola Law School, 2012. https://digitalcommons.lmu.edu/etd/419.
Texte intégralMoshaei, Mohammad Hossein. « Adhesion of Rolling Cell to Deformable Substrates in Shear Flow ». Ohio University / OhioLINK, 2018. http://rave.ohiolink.edu/etdc/view?acc_num=ohiou153373230467728.
Texte intégralKim, Jungtae. « Development of continuos flow micro cell sorter using immune magnetophoresis ». Aachen Shaker, 2008. http://d-nb.info/992481430/04.
Texte intégralKarp-Boss, Lee. « Phytoplankton-flow interactions in relation to cell size and morphology / ». Thesis, Connect to this title online ; UW restricted, 1998. http://hdl.handle.net/1773/11004.
Texte intégralPoon, Grace Chemical Sciences & Engineering Faculty of Engineering UNSW. « Bromine complexing agents for use in vanadium bromide (V/Br) redox flow cell ». Publisher:University of New South Wales. Chemical Sciences & ; Engineering, 2008. http://handle.unsw.edu.au/1959.4/41210.
Texte intégralPomalis, Richard. « A fundamental study of Starling flow and protein redistribution within a cell-free rectilinear membrane flow device ». Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1997. http://www.collectionscanada.ca/obj/s4/f2/dsk2/ftp04/mq20982.pdf.
Texte intégralSawyer, Frederick Emile. « Coupled mixing-cell and mass balance flow path models of the White River Flow System, Nevada, USA ». abstract and full text PDF (UNR users only), 2009. http://0-gateway.proquest.com.innopac.library.unr.edu/openurl?url_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:dissertation&res_dat=xri:pqdiss&rft_dat=xri:pqdiss:1467765.
Texte intégralFACCHINETTI, IRENE. « Thermally Regenerable Redox-Flow Batteries ». Doctoral thesis, Università degli Studi di Milano-Bicocca, 2021. http://hdl.handle.net/10281/308694.
Texte intégralLow-Temperature Heat (LTH), below of 100°C, has elicited great interest among the scientific community, as a source of energy since it does not see any form of utilization as it is currently simply released into the environment. Its conversion would open the doors to the exploitation of a huge amount of energy as well, such as geothermal, solar, and industrial waste heat. The conversion efficiencies of LTH are low because of the limitations imposed by Carnot law, as well as the existence of technological limits which further reduce the efficiency of the conversion of LTH. In order to be suitable for extensive industrial production, LTH converters should show high power densities, scalable and efficient whilst being cost-effective; to this point, the devices proposed for this afore mentioned application all failed to achieve suitable efficiencies and power density, making the LTH conversion unfeasible. This PhD project was focused on the design of a device called Thermally Regenerable Redox-Flow Battery (TRB) consisting of a redox-flow battery that can be recharged by a thermal process. The device is based upon a two-stages technology composed by a “power production” stage and a “thermal” stage: power production happens in an electrochemical cell which release electricity at the expenses of the mixing free energy of two water solutions of the same salt at different concentrations, referred to as a concentration cell. When the two solutions reach the same concentration, the exhausted fluid is sent to the second stage, the thermal process, which regenerates the initial mixing free energy, by exploiting LTH sources, through vacuum distillation. The efficiency of the technology is the product between the efficiencies of the units in the device where both stages happen: the electrochemical cell, engineered for power production, and a distillation unit, designed to be responsible for thermal conversion. NaI/I2 and LiBr/Br2 water solutions will be the most discussed redox couple in this thesis, as result of thermodynamic analysis that have shown the importance related to the solvent and salt choice to ensure high energy conversion efficiencies. The achieved results, as well as the main research activities, are briefly reported here: starting from the determination of the activity coefficients, mixing free energy of the initial solutions, and the open circuit voltage of the electrochemical are calculated. Electrochemical cells are specifically designed for both systems while electrochemical tests are performed to evaluate the main performances of the devices, such as power density and electrochemical efficiency. Modeling of the operational conditions of the thermal stage allows to determine the distillation efficiency for both the solutions. The initial experiments prove an unprecedented heat-to-electricity efficiency for both the systems: 3% for TRB-NaI and 4-5% for TRB based on LiBr, depending on the thickness of the membrane with a power density output of almost 10 W m-2 for both technologies, which opens various possibilities to implement further improvements into this new class of energy storage/converter devices.
Berdugo, Claudia. « Cell Damage Mechanisms and Stress Response in Animal Cell Culture ». The Ohio State University, 2010. http://rave.ohiolink.edu/etdc/view?acc_num=osu1269467441.
Texte intégral