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Bibliographies thématiques / Fluorescence Microscopy, Image Correlation Spectroscopy / Thèses

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Thèses sur le sujet « Fluorescence Microscopy, Image Correlation Spectroscopy »

Auteur : Grafiati

Publié le 9 mars 2023

Mis à jour le 19 juillet 2025

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1

Nicovich, Philip R. "Widefield fluorescence correlation spectroscopy." Diss., Georgia Institute of Technology, 2010. http://hdl.handle.net/1853/33849.

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Fluorescence correlation spectroscopy has become a standard technique for modern biophysics and single molecule spectroscopy research. Here is presented a novel widefield extension of the established single-point technique. Flow in microfluidic devices was used as a model system for microscopic motion and through widefield fluorescence correlation spectroscopy flow profiles were mapped in three dimensions. The technique presented is shown to be more tolerant to low signal strength, allowing image data with signal-to-noise values as low as 1.4 to produce accurate flow maps as well as utilizi

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BOUZIN, MARGAUX. "Correlazione di Immagini per lo Studio di Processi Dinamici in Sistemi Biologici." Doctoral thesis, Università degli Studi di Milano-Bicocca, 2015. http://hdl.handle.net/10281/94231.

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Dynamic processes are ubiquitous in biological systems: the transport of organelles, proteins and cargoes in the micron-sized heterogeneous cellular environment mainly occurs by Brownian diffusion, while directional flow or drift phenomena contribute to enhance the diffusion-mediated intracellular trafficking and are responsible for the delivery of blood, nutrients and signaling molecules on the larger scale of whole tissues and organs. Motivated by the relevance of transport phenomena in several fields ranging from cell biology to immunology, I adopt and extend the approach of Fluorescence Im

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3

Gallagher, Joseph. "Adaptive optics for fluorescence correlation spectroscopy." Thesis, Université Grenoble Alpes (ComUE), 2017. http://www.theses.fr/2017GREAY054/document.

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Ce projet de recherche conjugue deux aspects complémentaires : le développement d’un montage de microscopie intégrant un système d'Optique Adaptative (OA) et l’étude de masses cancéreuses (Sphéroïdes Multicellulaires) sous pression mécanique.Ces deux axes seront mutuellement bénéfiques puisque l’implémentation de l’OA rendra possible l’imagerie et les mesures physiques au sein des sphéroïdes ; d’un autre côté, l’étude des sphéroïdes permettra de caractériser les aberrations induites par ce type d’échantillons et de mieux comprendre les exigences sur le système d’OA qu’imposent l’observation de

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4

Doroshenko, Mikheil [Verfasser]. "Diffusion in heterogeneous systems studied by laser scanning confocal microscopy and fluorescence correlation spectroscopy / Mikheil Doroshenko." Mainz : Universitätsbibliothek Mainz, 2014. http://d-nb.info/104870758X/34.

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5

Xu, Lei. "Development and application of ultra-sensitive fluorescence spectroscopy and microscopy for biomolecular interaction studies." Doctoral thesis, KTH, Experimentell biomolekylär fysik, 2014. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-146181.

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This thesis describes the development of sensitive and high-resolution fluorescence spectroscopic and microscopic techniques and their application to probe biomolecules and their interactions in solution, lipid membrane model systems and in cells. Paper I-IV are largely focused on methodological developments. In paper I, a new fluorescence method based on fluorescence correlation spectroscopy (FCS) for detecting single particles was realized, requiring no fluorescent labeling of the particles. The method can yield information both about the diffusion properties of the particles as well as abou

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6

Wang, Ruixing. "STED-fluorescence correlation spectroscopy for dynamic observations in cell biology : from theoretical to practical approaches." Thesis, Aix-Marseille, 2018. http://www.theses.fr/2018AIXM0163/document.

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Les techniques de super-résolution offrent un nouvel aperçu de la description de l'organisation moléculaire dynamique de la membrane plasmique. Parmi ces techniques, la microscopie par déplétion d'émission stimulée (stimulated emission depletion, STED) dépasse la limite de diffraction optique et atteint une résolution de quelques dizaines de nanomètres. Il est une technique polyvalente qui peut être combinée avec d'autres techniques telles que la spectroscopie par corrélation de fluorescence (fluorescence correlation spectroscopy, FCS), fournissant des résolutions spatiales et temporelles élev

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7

Persson, Gustav. "Temporal Modulation in Fluorescence Spectroscopy and Imaging for Biological Applications." Doctoral thesis, KTH, Experimentell biomolekylär fysik, 2009. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-10243.

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This thesis explores the benefits of intensity modulation for the purpose of extending the range of applications of fluorescence spectroscopy and imaging in cellular and molecular biology and medicine. Long-lived transient states of fluorescent molecules can, because of their long lifetimes, be used to detect subtle changes in the microenvironment of the molecule. A method for determining the kinetic rates for transitions to and from such states by registration of changes in the average fluorescence intensity related to different modulation of the excitation source is introduced. It combines t

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8

Le, Andy Vinh. "Blood Microflow Characterization Using Micro-Particle Image Velocimetry and 2-Beam Fluorescence Cross-Correlation Spectroscopy." Thesis, Université d'Ottawa / University of Ottawa, 2020. http://hdl.handle.net/10393/41535.

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Blood flow through microcirculation in both simple and complex geometry has been difficult to predict due to the composition and complex behavior of blood at the microscale. Blood is a dense suspension of deformable red blood cells that is comparable in dimensions to the microchannels that it flows through. As a result, rheological properties at the microscale can vastly differ from bulk rheological properties due to non-continuum effects. To further develop our understanding of blood microflow; experimental techniques should be explored. In this work, we explore micro-particle image velocime

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9

Vaillancourt, Benoit. "Novel biophysical appliations [sic] of STICS." Thesis, McGill University, 2008. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=111550.

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The object of this thesis is to present two novel applications of Spatiotemporal Image Correlation Spectroscopy (STICS) to biological systems. STICS is a technique which uses the correlations in pixel intensity fluctuations of an image time series, captured under fluorescence microscopy, to measure the speed and direction of a flowing population of fluorescently labeled molecules. The method was first applied to measure the dynamics of transport vesicles inside growing pollen tubes of lily flowers. The measured vector maps allowed to confirm the presence of actin filaments along the periphery

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10

Kohram, Maryam. "A Combined Microscopy and Spectroscopy Approach to Study Membrane Biophysics." University of Akron / OhioLINK, 2015. http://rave.ohiolink.edu/etdc/view?acc_num=akron1436530389.

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11

Reitan, Nina Kristine. "Methods for studying critical barriers to the delivery of nanomedicine : The potential of fluorescence correlation spectroscopy, confocal microscopy and MRI." Doctoral thesis, Norges teknisk-naturvitenskapelige universitet, Institutt for fysikk, 2009. http://urn.kb.se/resolve?urn=urn:nbn:no:ntnu:diva-5760.

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12

Pu, Mingming. "Investigations of the Mechanism for Activation of Bacillus Thuringiensis Phosphatidylinositol-specific Phospholipase C." Thesis, Boston College, 2009. http://hdl.handle.net/2345/1179.

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Thesis advisor: Mary F. Roberts<br>Thesis advisor: Steven D. Bruner<br>The bacterial phosphatidylinositol-specific phospholipase C (PI-PLC) from Bacillus thuringiensis is specifically activated by low concentrations of a non-substrate lipid, phosphatidylcholine (PC), presented as an interface. However, if the PC concentration in the interface is too high relative to substrate, the enzyme exhibits surface dilution inhibition. Understanding this bacterial enzyme, which shares many kinetic features with the larger and more complex mammalian PI-PLC enzymes, requires elucidating the mechanism for P

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13

BONAIUTI, PAOLO. "A FISTFUL OF MOLECULES: CELLS ESCAPE AN OPERATIONAL MITOTIC CHECKPOINT THROUGH A STOCHASTIC PROCESS." Doctoral thesis, Università degli Studi di Milano, 2018. http://hdl.handle.net/2434/554699.

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The cell cycle culminates with the segregation of sister chromatids, which is a fundamental step in ensuring the transmission of unaltered genetic material. Chromosome segregation is carried out by the mitotic spindle, which captures and pulls sister chromatids towards the opposite poles. Anaphase starts when the correct bipolar attachment is achieved. Chromosomes migrate evenly to the two daughter cells, both inheriting the same genetic material. The presence of unattached kinetochore at anaphase onset is dangerous, since it may lead to unbalanced ploidy of daughter cells, with severe conse

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14

Tsutae, Fernando Massayuki. "Espectroscopia de correlação de fluorescência aplicada em estudos de sistemas moleculares, biológicos e celulares." Universidade de São Paulo, 2016. http://www.teses.usp.br/teses/disponiveis/76/76132/tde-14102016-101124/.

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A espectroscopia de correlação de fluorescência (FCS) é uma das diferentes técnicas de análise por imagens de alta resolução espacial e temporal de biomoléculas em concentrações extremamente baixas. Ela se tornou uma técnica extremamente poderosa e sensível em áreas como bioquímica e biofísica. Como uma técnica bem estabelecida, ela é utilizada para medir concentrações locais de biomoléculas, através da marcação com moléculas fluorescentes. Coeficientes de difusão e constantes cinéticas também podem ser medidos através de FCS assim como detecção de molécula única. Ela também pode dar informaçã

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15

CAINERO, ISOTTA. "Nanoscale investigation of chromatin organization by structured illumination microscopy." Doctoral thesis, Università degli studi di Genova, 2021. http://hdl.handle.net/11567/1042958.

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This thesis work aims to present a novel approach to reconstruct Structured Illumination Microscopy, SIM, raw data and to analyze SIM reconstructed images. These new approaches will be demonstrated in the study of chromatin organization. The dissertation will be articulated as follows: Chapter 1 provides an introduction to chromatin nanoscale organization and optical fluorescence microscopy, which is one of the main tools involved in life sciences studies. Indeed, optical microscopy allowed investigating, with high specificity and sensitivity, living samples such as cells, and even tissues.

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16

Baker, Lawrence R. "Spectroscopic Study of Compressible Mobile Phase and Stationary Phase Behavior in Chromatography." Diss., CLICK HERE for online access, 2008. http://contentdm.lib.byu.edu/ETD/image/etd2581.pdf.

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17

Chen, Chen. "SINGLE-MOLECULE ANALYSIS OF ALZHEIMER'S β-PEPTIDE OLIGOMER DISASSEMBLY AT PHYSIOLOGICAL CONCENTRATION". UKnowledge, 2014. http://uknowledge.uky.edu/chemistry_etds/31.

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The diffusible soluble oligomeric amyloid β-peptide (Aβ) has been identified as a toxic agent in Alzheimer’s disease that can cause synaptic dysfunction and memory loss, indicating its role as potential therapeutic targets for AD treatment. Recently an oligomer-specific sandwich biotin-avidin interaction based assay identified the Aβ oligomer dissociation potency of a series of dihydroxybenzoic acid (DHBA) isomers. Because the sandwich assay is an ensemble method providing limited size information, fluorescence correlation spectroscopy (FCS) was employed to provide single molecule resolution o

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18

Rouger, Vincent. "Développements méthodologiques pour l'exploration spatio-temporelle des mécanismes de transduction du signal." Thesis, Aix-Marseille, 2013. http://www.theses.fr/2013AIXM4056.

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La membrane plasmique constitue la première entité séparant la cellule de son environnement. A ce rôle de barrière s'ajoute celui de réguler la. Par conséquent, la membrane plasmique est une zone privilégiée pour le passage d'information. Cependant, son étude reste difficile, ne serait-ce que par l'extraordinaire complexité d'organisation de cet assemblage supramoléculaire.Mon projet de thèse vise à développer de nouvelles approches expérimentales pour explorer plus spécifiquement l'organisation et le rôle de la membrane plasmique d'une cellule dans les mécanismes de transduction de l'informat

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19

MORTATI, LEONARDO MICHAEL. "Coherent Anti-Stokes Raman Scattering, Second Harmonic Generation and Two-Photon Excitation Fluorescence Multimodal Microscope: Realization, Metrological Characterization and Applications in Regenerative Medicine." Doctoral thesis, Politecnico di Torino, 2013. http://hdl.handle.net/11583/2509905.

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In the frame of the research in biology and in particular in regenerative medicine, it is widely requested the ability to perform measurements that have a low impact on the observed biological systems. Many measurements imply sample modifications and also sample fixation avoiding living samples measurements. In this doctoral thesis it is presented the realization of an advanced optical multimodal microscope that integrates coherent anti-Stokes Raman scattering, second harmonic generation and two-photon excitation fluorescence techniques in a single powerful tool. The combination of all these m

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20

Vašíček, David. "Fluorescenční zobrazovací techniky v multimodálním holografickém mikroskopu." Master's thesis, Vysoké učení technické v Brně. Fakulta strojního inženýrství, 2014. http://www.nusl.cz/ntk/nusl-231466.

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The diploma thesis deals with the registration of images taken with the multimodal holographic microscope (MHM). The summary covers the fluorescent and holographic microscopy, and the multimodal holographic microscope combining both these microscopy types. Every pair of the images needs to be aligned in order to gain new information by combining both image types. The thesis contains an algorithm that registers images by phase correlation as well as a process created in MATLAB in accordance with the algorithm. The most important procedure parameters’ influence on the registration success is des

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21

Chwastek, Grzegorz. "Interactions of FCHo2 with lipid membranes." Doctoral thesis, Saechsische Landesbibliothek- Staats- und Universitaetsbibliothek Dresden, 2013. http://nbn-resolving.de/urn:nbn:de:bsz:14-qucosa-129388.

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Endocytosis is one of the most fundamental mechanisms by which the cell communicates with its surrounding. Specific signals are transduced through the cell membrane by a complex interplay between proteins and lipids. Clathrin depended endocytosis is one of important signalling pathways which leads to budding of the plasmalemma and a formation of endosomes. The FCHo2 is an essential protein at the initial stage of the this process. In is a membrane binding protein containing BAR (BIN, Amphiphysin, Rvs) domain which is responsible for a membrane binding. Although numerous valuable work on BAR pr

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22

Gennerich, Arne. "Fluoreszenzkorrelationsspektroskopie und Rasterkorrelationsmikroskopie molekularer Prozesse in Nervenzellen." Doctoral thesis, [S.l.] : [s.n.], 2003. http://deposit.ddb.de/cgi-bin/dokserv?idn=971029008.

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23

Ring, Josh. "Novel fabrication and testing of light confinement devices." Thesis, University of Manchester, 2016. https://www.research.manchester.ac.uk/portal/en/theses/novel-fabrication-and-testing-of-light-confinement-devices(51572720-0c49-482e-8523-e44ca877117f).html.

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The goal of this project is to study novel nanoscale excitation volumes, sensitive enoughto study individual chromophores and go on to study new and exciting self assemblyapproaches to this problem. Small excitation volumes may be engineered using light con-finement inside apertures in metal films. These apertures enhance fluorescence emissionrates, quantum yields, decrease fluorescence quenching, enable higher signal-to-noiseratios and allow higher concentration single chromophore fluorescence, to be studied byrestricting this excitation volume. Excitation volumes are reported on using the ch

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24

Täuber, Daniela. "Characterization of heterogeneous diffusion in confined soft matter." Doctoral thesis, Universitätsbibliothek Chemnitz, 2011. http://nbn-resolving.de/urn:nbn:de:bsz:ch1-qucosa-77658.

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A new method, probability distribution of diffusivities (time scaled square displacements between succeeding video frames), was developed to analyze single molecule tracking (SMT) experiments. This method was then applied to SMT experiments on ultrathin liquid tetrakis(2-ethylhexoxy)silane (TEHOS) films on Si wafer with 100 nm thermally grown oxide, and on thin semectic liquid crystal films. Spatial maps of diffusivities from SMT experiments on 220 nm thick semectic liquid crystal films reveal structure related dynamics. The SMT experiments on ultrathin TEHOS films were complemented by fluores

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Khmelinskaia, Alena. "Protein-monolayer interactions investigated by fluorescence microscopy and correlation spectroscopy." Master's thesis, 2013. http://hdl.handle.net/10451/9654.

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Tese de mestrado em Bioquímica, apresentada à Universidade de Lisboa, através da Faculdade de Ciências, 2013<br>A membrana celular é composta por uma ampla variedade de lípidos e proteínas. Através do estabelecimento de interacções entre lípidos e proteínas, quer membranares quer solúveis, podem ser originadas heterogeneidades espaciais, levando à formação de ‘jangadas lipídicas’. Estes domínios transitórios desempenham um papel fundamental em diferentes cadeias de sinalização celular. Apesar da investigação desenvolvida na área, a membrana celular é ainda hoje um dos componentes celulares men

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Liu, Ching-Chuan, and 劉慶川. "Investigations on Hydrodynamic Focusing by Con-focal Microscopy and Fluorescence Correlation Spectroscopy." Thesis, 2008. http://ndltd.ncl.edu.tw/handle/42817093147629453977.

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碩士<br>國立臺灣大學<br>應用力學研究所<br>96<br>In protein folding, early folding events occur on a microsecond to second time scale. In order to study protein kinetics, folding reactions must be triggered in a short time. In this thesis, we design four different micro-mixers based on hydrodynamic focusing. We use con-focal microscopy and fluorescence correlation spectroscopy (FCS) to synthesize qualitatively and quantitatively. Velocity is the most important parameter since it deeply affects the determination of mixing dead time and time resolution of our micro-mixer. Fluorescence correlation spectroscopy i

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Ghosh, Shirsendu. "Study of Niosomes, Proteins and Live Cell using Fluorescence Correlation Spectroscopy and Microscopy." Thesis, 2019. http://hdl.handle.net/10821/8217.

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In this thesis, we have focused our attention on three different process, - diffusion of organic dyes, conformation dynamics of protein and solvation dynamics. Diffusion of organic dyes provides important information on the micro-viscosity and heterogeneity of the system. We have determined the diffusion coefficient and heterogeneity inside a niosome using fluorescence correlation spectroscopy. We also determined the micro-viscosity of different region of a live cell by measuring the diffusion coefficient of dyes in those regions. We studied the effect of solvent mixtures, such as binary mixtu

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Amin, Md Asif. "Time Resolved Spectroscopy and Microscopy: Application to Live Cells and Related Systems." Thesis, 2019. http://hdl.handle.net/10821/8243.

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In this thesis, we have applied time resolved confocal microscopy to five different processes, - intermittent oscillation of lipid droplets in live cells14, in situ imaging of cancer cell by fluorescent gold nanoclusters, selective killing of breast cancer cells, delivery of cytochrome c and hence imaging of live cell and effect of binary solvent mixture on structure and dynamics of a protein. Intermittent oscillation in live lung cell is observed using time resolved confocal microscopy and we have observed significant differences between normal and cancer cell. Imaging of cancer cell was done

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Sen, Mojumdar Supratik. "STUDY OF ORGANIZED MEDIA USING FEMTOSECOND SPECTROSCOPY AND CONFOCAL MICROSCOPY." Thesis, 2019. http://hdl.handle.net/10821/8311.

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Self-organized nanostructures play a crucial role in a wide range of natural phenomena ranging from molecular recognition (e.g. enzyme-substrate, antigenantibody interaction), to a more general case of unusual chemistry in confined environments. Due to the confinement and local interaction, the biological activity and dynamics in these assemblies are significantly different from those in ordinary solutions. In recent years, ultrafast time resolved spectroscopy revealed substantial amount of new information on these systems.The experimented results has stimulated a large body theoretical studie

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30

Godoy, Vargas Jazmin. "Synthesis and Tracking of Fluorescent and Polymerization-Propelled Single-Molecule Nanomachines." Thesis, 2012. http://hdl.handle.net/1911/71652.

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This dissertation describes the synthesis of molecular machines designed to operate on surfaces (nanocars) or in the solution phase (nanosubmarines), and the study of their diffusion using fluorescence techniques. The design of these molecular machines is aimed to facilitate monitoring of their movement and incorporation of a source of energy for propulsion. To complement previous scanning tunneling microscopy studies of the translation of nanocars on surfaces, chapter 1 describes the synthesis of a family of fluorescently tagged nanocars. The nanocars were functionalized with a tetramethyl

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31

Isbaner, Sebastian. "Extending Resolution in All Directions: Image Scanning Microscopy and Metal-induced Energy Transfer." Doctoral thesis, 2019. http://hdl.handle.net/11858/00-1735-0000-002E-E5D6-C.

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32

Schilders, Steven Paul. "Microscopic imaging in turbid media." Thesis, 1999. https://vuir.vu.edu.au/15683/.

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This thesis is concerned with the understanding of photon propagation within turbid media with different characteristics (e.g. scattering anisotropy, optical thickness and geometric thickness). The turbid media (samples) used in the work presented in this thesis consist of polystyrene microspheres and semi-skimmed milk suspensions. In particular the work presented in this thesis is concerned with the development of novel microscopic imaging techniques that allow high quality (i.e. micrometer scale resolution, high contrast and have a high signal-to-noise ratio) imaging through turbid media. T

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Maillette, Sébastien. "Agglomération et hétéroagglomération des nanoparticules d'argent en eaux douces." Thèse, 2015. http://hdl.handle.net/1866/12516.

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Les nanomatériaux sont une classe de contaminants qui est de plus en plus présent dans l’environnement. Leur impact sur l’environnement dépendra de leur persistance, mobilité, toxicité et bioaccumulation. Chacun de ces paramètres dépendra de leur comportement physicochimique dans les eaux naturelles (i.e. dissolution et agglomération). L’objectif de cette étude est de comprendre l’agglomération et l’hétéroagglomération des nanoparticules d’argent dans l’environnement. Deux différentes sortes de nanoparticules d’argent (nAg; avec enrobage de citrate et avec enrobage d’acide polyacrylique) de 5

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Chwastek, Grzegorz. "Interactions of FCHo2 with lipid membranes." Doctoral thesis, 2012. https://tud.qucosa.de/id/qucosa%3A27325.

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Endocytosis is one of the most fundamental mechanisms by which the cell communicates with its surrounding. Specific signals are transduced through the cell membrane by a complex interplay between proteins and lipids. Clathrin depended endocytosis is one of important signalling pathways which leads to budding of the plasmalemma and a formation of endosomes. The FCHo2 is an essential protein at the initial stage of the this process. In is a membrane binding protein containing BAR (BIN, Amphiphysin, Rvs) domain which is responsible for a membrane binding. Although numerous valuable work on BAR pr

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