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Bibliographies thématiques / Fluorescence Microscopy, Image Correlation Spectroscopy / Articles de revues

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Articles de revues sur le sujet « Fluorescence Microscopy, Image Correlation Spectroscopy »

Auteur : Grafiati

Publié le 9 mars 2023

Mis à jour le 19 juillet 2025

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1

Clayton, Andrew H. A. "Phase-Sensitive Fluorescence Image Correlation Spectroscopy." International Journal of Molecular Sciences 25, no. 20 (2024): 11165. http://dx.doi.org/10.3390/ijms252011165.

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Fluorescence lifetime imaging microscopy is sensitive to molecular interactions and environments. In homo-dyne frequency-domain fluorescence lifetime imaging microscopy, images of fluorescence objects are acquired at different phase settings of the detector. The detected intensity as a function of detector phase is a sinusoidal function that is sensitive to the lifetime of the fluorescent species. In this paper, the theory of phase-sensitive fluorescence image correlation spectroscopy is described. In this version of lifetime imaging, image correlation spectroscopy analysis (i.e., spatial auto

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Wiseman, Paul. "Introduction to Fluorescence and Image Correlation Spectroscopy." Microscopy and Microanalysis 10, S02 (2004): 246–47. http://dx.doi.org/10.1017/s1431927604886483.

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Bates, Ian R., Paul W. Wiseman, and John W. Hanrahan. "Investigating membrane protein dynamics in living cellsThis paper is one of a selection of papers published in this Special Issue, entitled CSBMCB — Membrane Proteins in Health and Disease." Biochemistry and Cell Biology 84, no. 6 (2006): 825–31. http://dx.doi.org/10.1139/o06-189.

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Live cell imaging is a powerful tool for understanding the function and regulation of membrane proteins. In this review, we briefly discuss 4 fluorescence-microscopy-based techniques for studying the transport dynamics of membrane proteins: fluorescence-correlation spectroscopy, image-correlation spectroscopy, fluorescence recovery after photobleaching, and single-particle and (or) molecule tracking. The advantages and limitations of each approach are illustrated using recent studies of an ion channel and cell adhesion molecules.

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Wiseman, P. W., J. C. Bouwer, S. Peltier, and M. H. Ellisman. "High Speed Two Photon Excitation Microscopy in Live Cell Imaging using Image Correlation Spectroscopy (ICS)." Microscopy and Microanalysis 7, S2 (2001): 22–23. http://dx.doi.org/10.1017/s1431927600026180.

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For live-cell imaging, two-photon excitation microscopy (TPEM) is proving to be a significant technological advancement. The unique features offered by TPEM are the ability to image thick sections, excellent optical sectioning capabilities, low damage to living cells, and less out of focus fluorescence and out of focus photobleaching. of these features, the most useful for the biological microscopist, is optical sectioning. Optical sectioning is an intrinsic property of the two-photon process, whereby, two infrared (IR) photons are absorbed quickly to excite a single UV/blue transition. The pr

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Laňková, Martina, Jana Humpolíčková, Stanislav Vosolsobě, et al. "Determination of Dynamics of Plant Plasma Membrane Proteins with Fluorescence Recovery and Raster Image Correlation Spectroscopy." Microscopy and Microanalysis 22, no. 2 (2016): 290–99. http://dx.doi.org/10.1017/s1431927616000568.

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AbstractA number of fluorescence microscopy techniques are described to study dynamics of fluorescently labeled proteins, lipids, nucleic acids, and whole organelles. However, for studies of plant plasma membrane (PM) proteins, the number of these techniques is still limited because of the high complexity of processes that determine the dynamics of PM proteins and the existence of cell wall. Here, we report on the usage of raster image correlation spectroscopy (RICS) for studies of integral PM proteins in suspension-cultured tobacco cells and show its potential in comparison with the more wide

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Diaspro, Alberto, Giuseppe Chirico, and Maddalena Collini. "Two-photon fluorescence excitation and related techniques in biological microscopy." Quarterly Reviews of Biophysics 38, no. 2 (2005): 97–166. http://dx.doi.org/10.1017/s0033583505004129.

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1. Introduction 982. Historical background of two-photon effects 992.1 2PE 1002.2 Harmonic generation 1002.3 Fluorescence correlation spectroscopy 1003. Basic principles of two-photon excitation of fluorescent molecules and implications for microscopy and spectroscopy 1013.1 General considerations 1013.2 Fluorescence intensity under the 2PE condition 1033.3 Optical consequences of 2PE 1043.4 Saturation effects in 2PE 1083.5 Fluorescence correlation spectroscopy 1093.5.1 Autocorrelation analysis 1103.5.2 Photon-counting histogram analysis 1124. Two-photon-excited probes 1155. Design considerati

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Friaa, Ouided, and Cécile Fradin. "Coincidence Measurements in Dual-Color Confocal Microscopy: A Combined Single-Particle and Fluorescence Correlation Approach." Biophysical Reviews and Letters 09, no. 03 (2014): 249–71. http://dx.doi.org/10.1142/s1793048014400074.

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In this paper we discuss how the coincident detection of mobile particles in dual-color confocal images can be improved. Optimal coincidence detection requires a careful choice of experimental conditions and image acquisition parameters in order to maximize the overlap between the two detection volumes. By measuring this overlap with fluorescence cross-correlation spectroscopy, we show in particular that a small confocal field of view is necessary in order to maintain good coincidence. Most importantly, coincidence detection also requires a dedicated image analysis strategy. Traditionally, two

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Pelicci, Simone, Laura Furia, Mirco Scanarini, Pier Giuseppe Pelicci, Luca Lanzanò, and Mario Faretta. "Novel Tools to Measure Single Molecules Colocalization in Fluorescence Nanoscopy by Image Cross Correlation Spectroscopy." Nanomaterials 12, no. 4 (2022): 686. http://dx.doi.org/10.3390/nano12040686.

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Super Resolution Microscopy revolutionized the approach to the study of molecular interactions by providing new quantitative tools to describe the scale below 100 nanometers. Single Molecule Localization Microscopy (SMLM) reaches a spatial resolution less than 50 nm with a precision in calculating molecule coordinates between 10 and 20 nanometers. However new procedures are required to analyze data from the list of molecular coordinates created by SMLM. We propose new tools based on Image Cross Correlation Spectroscopy (ICCS) to quantify the colocalization of fluorescent signals at single mole

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Pandzic, E., and R. M. Whan. "A Practical Guide to Fluorescence Temporal and Spatial Correlation Spectroscopy." Biophysicist 2, no. 1 (2021): 40–69. http://dx.doi.org/10.35459/tbp.2019.000143.

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ABSTRACT The aim of this article is to introduce the basic principles behind the widely used microscopy tool: fluorescence fluctuation correlation spectroscopy (FFCS). We present the fundamentals behind single spot acquisition (FCS) and its extension to spatiotemporal sampling, which is implemented through image correlation spectroscopy (ICS). The article is an educational guide that introduces theoretic concepts of FCS and some of the ICS techniques, followed by interactive exercises in MATLAB. There, the learner can simulate data time series and the application of various FFCS techniques, as

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Dal Fovo, Alice, Margherita Morello, Anna Mazzinghi, Caterina Toso, Monica Galeotti, and Raffaella Fontana. "Spectral Mapping Techniques for the Stratigraphic and Compositional Characterisation of a 16th-Century Painting." Heritage 7, no. 3 (2024): 1320–33. http://dx.doi.org/10.3390/heritage7030063.

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Identifying a painting’s pigment palette is crucial for comprehending the author’s technique, as well as for evaluating the degradation of the materials. This paper investigates the stratigraphy and pigments distribution of a 16th-century painting from the Uffizi Galleries collection. Firstly, we obtained compositional information through the cross-sectional analysis of samples using scanning electron microscopy. Secondly, we performed elemental mapping using macro-X-ray fluorescence followed by reflectance imaging spectroscopy. The painting image cube was analysed using the spectral correlati

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Moens, Pierre D. J., Enrico Gratton, and Iyrri L. Salvemini. "Fluorescence correlation spectroscopy, raster image correlation spectroscopy, and number and brightness on a commercial confocal laser scanning microscope with analog detectors (Nikon C1)." Microscopy Research and Technique 74, no. 4 (2010): 377–88. http://dx.doi.org/10.1002/jemt.20919.

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Cao, Yifan, Behzad Fuladpanjeh-Hojaghan, Milana Trifkovic, and Edward P. L. Roberts. "Operando Visualization of Redox Flow Batteries Using Fluorescence Spectroscopy with Alizarin Red S Electrolyte." ECS Meeting Abstracts MA2024-01, no. 3 (2024): 595. http://dx.doi.org/10.1149/ma2024-013595mtgabs.

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Redox flow batteries (RFBs) charge and discharge by transferring electrons between two pairs of redox species separated by a proton exchange membrane. Unlike traditional batteries that store energy in redox-active materials within an enclosed cell, RFBs store energy in liquid electrolytes housed in external reservoirs1. These electrolytes are pumped through the cell, enabling redox reactions on the surface of electrodes. The energy storage capacity of RFBs is determined by the concentration of the active species and the size of the reservoirs, making them a promising technology for large-scale

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Waharte, François, Karine Steenkeste, Romain Briandet, and Marie-Pierre Fontaine-Aupart. "Diffusion Measurements inside Biofilms by Image-Based Fluorescence Recovery after Photobleaching (FRAP) Analysis with a Commercial Confocal Laser Scanning Microscope." Applied and Environmental Microbiology 76, no. 17 (2010): 5860–69. http://dx.doi.org/10.1128/aem.00754-10.

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ABSTRACT Research about the reactional and structural dynamics of biofilms at the molecular level has made great strides, owing to efficient fluorescence imaging methods in terms of spatial resolution and fast acquisition time but also to noninvasive conditions of observation consistent with in situ biofilm studies. In addition to conventional fluorescence intensity imaging, the fluorescence recovery after photobleaching (FRAP) module can now be routinely implemented on commercial confocal laser scanning microscopes (CLSMs). This method allows measuring of local diffusion coefficients in biofi

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Lou, Jieqiong, Lorenzo Scipioni, Belinda K. Wright, et al. "Phasor histone FLIM-FRET microscopy quantifies spatiotemporal rearrangement of chromatin architecture during the DNA damage response." Proceedings of the National Academy of Sciences 116, no. 15 (2019): 7323–32. http://dx.doi.org/10.1073/pnas.1814965116.

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To investigate how chromatin architecture is spatiotemporally organized at a double-strand break (DSB) repair locus, we established a biophysical method to quantify chromatin compaction at the nucleosome level during the DNA damage response (DDR). The method is based on phasor image-correlation spectroscopy of histone fluorescence lifetime imaging microscopy (FLIM)-Förster resonance energy transfer (FRET) microscopy data acquired in live cells coexpressing H2B-eGFP and H2B-mCherry. This multiplexed approach generates spatiotemporal maps of nuclear-wide chromatin compaction that, when coupled w

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Marshall, Wallace F. "Quantitative High-Throughput Assays for Flagella-Based Motility in Chlamydomonas Using Plate-Well Image Analysis and Transmission Correlation Spectroscopy." Journal of Biomolecular Screening 14, no. 2 (2009): 133–41. http://dx.doi.org/10.1177/1087057108328131.

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Cilia are motile and sensory organelles with important roles in human development, physiology, and disease. Genetic defects in cilia produce a host of disease symptoms, including polycystic kidney disease, hydrocephalus, retinal degeneration, chronic bronchiectasis, infertility, and polydactyly. Currently, there are no known drugs for pharmacological remediation of ciliary defects. Small-molecule modulators of ciliary assembly or function would provide potential lead compounds for drug discovery efforts and would immediately be invaluable tools for a chemical biology approach to studying cilia

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Khare, Devanshi, Pallavi Chandwadkar, and Celin Acharya. "Structural Analysis of Gliding Motility of a Bacteroidetes Bacterium by Correlative Light and Scanning Electron Microscopy (CLSEM)." Microscopy and Microanalysis 28, no. 2 (2022): 515–21. http://dx.doi.org/10.1017/s1431927622000095.

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The members of the Bacteroidetes phylum move on surfaces by gliding motility in the absence of external motility appendages, leading to the formation of spreading colonies. Here, the structural features of the spreading colony were assessed in a uranium-tolerant Bacteroidetes bacterium, Chryseobacterium sp. strain PMSZPI, by using correlative light and scanning electron microscopy (CLSEM). We developed a simple and convenient workflow for CLSEM using a shuttle and find software module and a correlative sample holding slide designed to transport samples between the light/fluorescence microscope

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Dobrinskikh, Evgenia, Luca Lanzano, Joanna Rachelson, et al. "Shank2 contributes to the apical retention and intracellular redistribution of NaPiIIa in OK cells." American Journal of Physiology-Cell Physiology 304, no. 6 (2013): C561—C573. http://dx.doi.org/10.1152/ajpcell.00189.2012.

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In renal proximal tubule (PT) cells, sodium-phosphate cotransporter IIa (NaPiIIa) is normally concentrated within the apical membrane where it reabsorbs ∼70% of luminal phosphate (Pi). NaPiIIa activity is acutely regulated by moderating its abundance within the apical membrane. Under low-Pi conditions, NaPiIIa is retained within the apical membrane. Under high-Pi conditions, NaPiIIa is retrieved from the apical membrane and trafficked to the lysosomes for degradation. The present study investigates the role of Shank2 in regulating the distribution of NaPiIIa. In opossum kidney cells, a PT cell

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Zhou, Mian, and Yu-Li Wang. "Distinct Pathways for the Early Recruitment of Myosin II and Actin to the Cytokinetic Furrow." Molecular Biology of the Cell 19, no. 1 (2008): 318–26. http://dx.doi.org/10.1091/mbc.e07-08-0783.

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Equatorial organization of myosin II and actin has been recognized as a universal event in cytokinesis of animal cells. Current models for the formation of equatorial cortex favor either directional cortical transport toward the equator or localized de novo assembly. However, this process has never been analyzed directly in dividing mammalian cells at a high resolution. Here we applied total internal reflection fluorescence microscope (TIRF-M), coupled with spatial temporal image correlation spectroscopy (STICS) and a new analytical approach termed temporal differential microscopy (TDM), to im

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Saha, Ipsita, and Saveez Saffarian. "Dynamics in HIV Gag Lattice Detected by Time-Lapse iPALM." Proceedings 50, no. 1 (2020): 65. http://dx.doi.org/10.3390/proceedings2020050065.

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Immature HIV virions have a lattice of Gag and Gag-Pol proteins anchored to the lumen of their envelope. This lattice has significant void spaces that were previously characterized by fluorescence correlation spectroscopy, cryoelectron tomography, and iPALM imaging. In the current study, we demonstrate that HIV virus-like particles (VLPs,) assembled by the viral protein Gag tagged at its C terminus with the photoactivable fluorescent protein Dendra, and are of the same size as virus-like particles assembled using only HIV Gag (140 ± 15 nm). We show that the Gag-Dendra lattice observed within t

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Majors, P. D., J. S. McLean, J. K. Fredrickson, and R. A. Wind. "NMR methods for in-situ biofilm metabolism studies: spatial and temporal resolved measurements." Water Science and Technology 52, no. 7 (2005): 7–12. http://dx.doi.org/10.2166/wst.2005.0174.

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We are developing novel nuclear magnetic resonance (NMR) microscopy, spectroscopy and combined NMR/optical techniques for the study of biofilms under known, controlled growth conditions. Objectives include: time and depth-resolved metabolite concentrations with isotropic spatial resolution on the order of 10 microns, metabolic pathways and flux rates, mass transport and ultimately their correlation with gene expression by optical microscopy in biofilms. We describe the implementation of ex-situ grown biofilms to improve growth environment control and NMR analysis. In-situ NMR depth resolved me

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Clayton, Andrew H. A. "Spectral Relaxation Imaging Microscopy II: Complex Dynamics." International Journal of Molecular Sciences 24, no. 15 (2023): 12271. http://dx.doi.org/10.3390/ijms241512271.

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The dynamics of condensed matter can be measured by the time-dependent Stokes shift of a suitable fluorescent probe. The time-dependent spectral correlation function is typically described by one or more spectral relaxation correlation times, which, in liquid solvents, characterize the timescales of the dipolar relaxation processes around the excited-state probe. The phasor plot provides a powerful approach to represent and analyze time and frequency-domain data acquired as images, thus providing a spatial map of spectral dynamics in a complex structure such as a living cell. Measurements of t

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Blumhardt, Philipp, Johannes Stein, Jonas Mücksch, et al. "Photo-Induced Depletion of Binding Sites in DNA-PAINT Microscopy." Molecules 23, no. 12 (2018): 3165. http://dx.doi.org/10.3390/molecules23123165.

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The limited photon budget of fluorescent dyes is the main limitation for localization precision in localization-based super-resolution microscopy. Points accumulation for imaging in nanoscale topography (PAINT)-based techniques use the reversible binding of fluorophores and can sample a single binding site multiple times, thus elegantly circumventing the photon budget limitation. With DNA-based PAINT (DNA-PAINT), resolutions down to a few nanometers have been reached on DNA-origami nanostructures. However, for long acquisition times, we find a photo-induced depletion of binding sites in DNA-PA

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Voskoboynikova, Natalia, Maria Karlova, Rainer Kurre, et al. "A Three-Dimensional Model of the Yeast Transmembrane Sensor Wsc1 Obtained by SMA-Based Detergent-Free Purification and Transmission Electron Microscopy." Journal of Fungi 7, no. 2 (2021): 118. http://dx.doi.org/10.3390/jof7020118.

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The cell wall sensor Wsc1 belongs to a small family of transmembrane proteins, which are crucial to sustain cell integrity in yeast and other fungi. Wsc1 acts as a mechanosensor of the cell wall integrity (CWI) signal transduction pathway which responds to external stresses. Here we report on the purification of Wsc1 by its trapping in water-soluble polymer-stabilized lipid nanoparticles, obtained with an amphipathic styrene-maleic acid (SMA) copolymer. The latter was employed to transfer tagged sensors from their native yeast membranes into SMA/lipid particles (SMALPs), which allows their pur

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Tudor, Mihaela, Roxana Cristina Popescu, Ionela N. Irimescu, et al. "Enhancing Proton Radiosensitivity of Chondrosarcoma Using Nanoparticle-Based Drug Delivery Approaches: A Comparative Study of High- and Low-Energy Protons." International Journal of Molecular Sciences 25, no. 21 (2024): 11481. http://dx.doi.org/10.3390/ijms252111481.

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To overcome chondrosarcoma’s (CHS) high chemo- and radioresistance, we used polyethylene glycol-encapsulated iron oxide nanoparticles (IONPs) for the controlled delivery of the chemotherapeutic doxorubicin (IONPDOX) to amplify the cytotoxicity of proton radiation therapy. Human 2D CHS SW1353 cells were treated with protons (linear energy transfer (LET): 1.6 and 12.6 keV/µm) with and without IONPDOX. Cell survival was assayed using a clonogenic test, and genotoxicity was tested through the formation of micronuclei (MN) and γH2AX foci, respectively. Morphology together with spectral fingerprints

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Kim, Lee, Fujii, et al. "Mitotic Chromosomes in Live Cells Characterized Using High-Speed and Label-Free Optical Diffraction Tomography." Cells 8, no. 11 (2019): 1368. http://dx.doi.org/10.3390/cells8111368.

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The cell nucleus is a three-dimensional, dynamic organelle organized into subnuclear compartments such as chromatin and nucleoli. The structure and function of these compartments are maintained by diffusion and interactions between related factors as well as by dynamic and structural changes. Recent studies using fluorescent microscopic techniques suggest that protein factors can access and are freely mobile in heterochromatin and in mitotic chromosomes, despite their densely packed structure. However, the physicochemical properties of the chromosome during cell division are not fully understo

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Fang, Weicheng, Xingxing Cheng, Chang-Jung Sun, Hongfei Xiao, and Jing Wang. "Optimization of the Fabrication of Sustainable Ceramsite Adsorbent from Coal Fly Ash/Waterworks Sludge/Waste Glass for Decolorization of Malachite Green." Adsorption Science & Technology 2023 (February 3, 2023): 1–13. http://dx.doi.org/10.1155/2023/8581697.

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As a traditional dye, malachite green (MG) poses a threat to our environment and health. To decolorize MG, a composite ceramsite adsorbent composed of coal fly ash (CFA), sewage treatment sludge (STS), and waste glass (WG) with a quality ratio of 3 : 3 : 4, respectively, was prepared. The optimal preparation parameters were determined as follows: preheating temperature = 600 ° C , sintering temperature = 1157 ° C , and sintering time = 17 min . Under optimal conditions, scanning electron microscopy (SEM) images show that the X-Com-ceramsite sample exhibits rough features and a porous structure

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Kim, Lee, Fujii, Kim, and Pack. "Physicochemical Properties of Nucleoli in Live Cells Analyzed by Label-Free Optical Diffraction Tomography." Cells 8, no. 7 (2019): 699. http://dx.doi.org/10.3390/cells8070699.

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The cell nucleus is three-dimensionally and dynamically organized by nuclear components with high molecular density, such as chromatin and nuclear bodies. The structure and functions of these components are represented by the diffusion and interaction of related factors. Recent studies suggest that the nucleolus can be assessed using various protein probes, as the probes are highly mobile in this organelle, although it is known that they have a densely packed structure. However, physicochemical properties of the nucleolus itself, such as molecular density and volume when cellular conditions ar

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Breusegem, Sophia Y., Moshe Levi, and Nicholas P. Barry. "Fluorescence Correlation Spectroscopy and Fluorescence Lifetime Imaging Microscopy." Nephron Experimental Nephrology 103, no. 2 (2006): e41-e49. http://dx.doi.org/10.1159/000090615.

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Macháň, Radek, Peter Kapusta, and Martin Hof. "Statistical filtering in fluorescence microscopy and fluorescence correlation spectroscopy." Analytical and Bioanalytical Chemistry 406, no. 20 (2014): 4797–813. http://dx.doi.org/10.1007/s00216-014-7892-7.

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Thompson, N. "Total Internal Reflection with Fluorescence Correlation Spectroscopy." Microscopy and Microanalysis 17, S2 (2011): 38–39. http://dx.doi.org/10.1017/s1431927611001061.

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Arkhipov, Anton, Jana Hüve, Martin Kahms, Reiner Peters, and Klaus Schulten. "Continuous Fluorescence Microphotolysis and Correlation Spectroscopy Using 4Pi Microscopy." Biophysical Journal 93, no. 11 (2007): 4006–17. http://dx.doi.org/10.1529/biophysj.107.107805.

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Watanabe, Takeshi, Yoshinori Iketaki, Takashige Omatsu, Kimihisa Yamamoto, and Masaaki Fujii. "Two-Point Separation in Far-Field Super-Resolution Fluorescence Microscopy Based on Two-Color Fluorescence Dip Spectroscopy, Part I: Experimental Evaluation." Applied Spectroscopy 59, no. 7 (2005): 868–72. http://dx.doi.org/10.1366/0003702054411562.

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The two-point resolution of a novel two-color far-field super-resolution fluorescence microscopy was evaluated by measuring fluorescent beads 100 nm in diameter. This microscopy is based on a combination of two-color fluorescence dip spectroscopy and a phase-modulation technique for a laser beam. By simply introducing two-color laser light, the size of the fluorescent image of a bead was shrunk down to a diameter of 250 nm from the diffraction-limited image with a diameter of 360 nm. For two closely adjacent fluorescent beads with a separation distance of 350 nm, the two-color microscope clear

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Abbandonato, Gerardo, Katrin Hoffmann, and Ute Resch-Genger. "Determination of quantum yields of semiconductor nanocrystals at the single emitter level via fluorescence correlation spectroscopy." Nanoscale 10, no. 15 (2018): 7147–54. http://dx.doi.org/10.1039/c7nr09332b.

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A microscopy-based method to determine fluorescence quantum yields Φ<sub>F</sub> of dispersed semiconductor nanocrystals at ultralow concentration with fluorescence correlation spectroscopy (FCS) is presented.

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Raarup, Merete Krog, and Jens Randel Nyengaard. "QUANTITATIVE CONFOCAL LASER SCANNING MICROSCOPY." Image Analysis & Stereology 25, no. 3 (2011): 111. http://dx.doi.org/10.5566/ias.v25.p111-120.

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This paper discusses recent advances in confocal laser scanning microscopy (CLSM) for imaging of 3D structure as well as quantitative characterization of biomolecular interactions and diffusion behaviour by means of one- and two-photon excitation. The use of CLSM for improved stereological length estimation in thick (up to 0.5 mm) tissue is proposed. The techniques of FRET (Fluorescence Resonance Energy Transfer), FLIM (Fluorescence Lifetime Imaging Microscopy), FCS (Fluorescence Correlation Spectroscopy) and FRAP (Fluorescence Recovery After Photobleaching) are introduced and their applicabil

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Morris, Hannah R., Clifford C. Hoyt, and Patrick J. Treado. "Imaging Spectrometers for Fluorescence and Raman Microscopy: Acousto-Optic and Liquid Crystal Tunable Filters." Applied Spectroscopy 48, no. 7 (1994): 857–66. http://dx.doi.org/10.1366/0003702944029820.

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Acousto-optic tunable filters (AOTF) and liquid crystal tunable filters (LCTF) are evaluated for their suitability as fluorescence microscopy imaging spectrometers. AOTFs are solid-state birefringent crystals that provide an electronically tunable spectral notch passband in response to an applied acoustic field. LCTFs also provide a notch passband that can be controlled by incorporating liquid crystal waveplate retarders within a Lyot birefringent filter. In this paper, spectroscopic performance and imaging quality are contrasted by evaluation of model systems. Studies include transmission ima

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Wu, Jun, Zachary R. Donly, Kevin J. Donly, and Steven Hackmyer. "Demineralization Depth Using QLF and a Novel Image Processing Software." International Journal of Dentistry 2010 (2010): 1–7. http://dx.doi.org/10.1155/2010/958264.

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Quantitative Light-Induced fluorescence (QLF) has been widely used to detect tooth demineralization indicated by fluorescence loss with respect to surrounding sound enamel. The correlation between fluorescence loss and demineralization depth is not fully understood. The purpose of this project was to study this correlation to estimate demineralization depth. Extracted teeth were collected. Artificial caries-like lesions were created and imaged with QLF. Novel image processing software was developed to measure the largest percent of fluorescence loss in the region of interest. All teeth were th

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Jones, Debbie L., Michael B. Andrews, Adam N. Swinburne, et al. "Fluorescence spectroscopy and microscopy as tools for monitoring redox transformations of uranium in biological systems." Chemical Science 6, no. 9 (2015): 5133–38. http://dx.doi.org/10.1039/c5sc00661a.

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Müller, Claus B., Kerstin Weiß, Walter Richtering, Anastasia Loman, and Joerg Enderlein. "Calibrating Differential Interference Contrast Microscopy with dual-focus Fluorescence Correlation Spectroscopy." Optics Express 16, no. 6 (2008): 4322. http://dx.doi.org/10.1364/oe.16.004322.

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Gray, J., S. Lockett, L. Mascio, et al. "Digital imaging microscopy for molecular cytogenetics." Proceedings, annual meeting, Electron Microscopy Society of America 52 (1994): 82–83. http://dx.doi.org/10.1017/s0424820100168141.

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Digital imaging microscopy is an essential tool in molecular cytogenetics. We describe here, hardware and software for sensitive multi-color image acquisition and display, gene mapping, generation ofcopy number karyotypes using comparative genomic hybridization (CGH), and correlation between genotype and histology in three dimensions.(a) QUantitative Image Processing System (QUIPS):Hardware We have developed a QUIPS that is now in routine use. This system is built around a commercial fluorescence microscope equipped with computer-controlled stage, focus and fluorescence excitation filter selec

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Zhang, Pengfei, Lingbo Kong, Guiwen Wang, Peter Setlow, and Yong-qing Li. "Monitoring the Wet-Heat Inactivation Dynamics of Single Spores of Bacillus Species by Using Raman Tweezers, Differential Interference Contrast Microscopy, and Nucleic Acid Dye Fluorescence Microscopy." Applied and Environmental Microbiology 77, no. 14 (2011): 4754–69. http://dx.doi.org/10.1128/aem.00194-11.

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ABSTRACTDynamic processes during wet-heat treatment of individual spores ofBacillus cereus,Bacillus megaterium, andBacillus subtilisat 80 to 90°C were investigated using dual-trap Raman spectroscopy, differential interference contrast (DIC) microscopy, and nucleic acid stain (SYTO 16) fluorescence microscopy. During spore wet-heat treatment, while the spores' 1:1 chelate of Ca2+with dipicolinic acid (CaDPA) was released rapidly at a highly variable timeTlag, the levels of spore nucleic acids remained nearly unchanged, and theTlagtimes for individual spores from the same preparation were increa

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James, Jeemol, Jonas Enger, and Marica B. Ericson. "Fluorescence Correlation Spectroscopy Combined with Multiphoton Laser Scanning Microscopy—A Practical Guideline." Applied Sciences 11, no. 5 (2021): 2122. http://dx.doi.org/10.3390/app11052122.

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Multiphoton laser scanning microscopy (MPM) has opened up an optical window into biological tissues; however, imaging is primarily qualitative. Cell morphology and tissue architectures can be clearly visualized but quantitative analysis of actual concentration and fluorophore distribution is indecisive. Fluorescence correlation spectroscopy (FCS) is a highly sensitive photophysical methodology employed to study molecular parameters such as diffusion characteristics on the single molecule level. In combination with laser scanning microscopy, and MPM in particular, FCS has been referred to as a

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Sánchez, Susana A., and Enrico Gratton. "Lipid−Protein Interactions Revealed by Two-Photon Microscopy and Fluorescence Correlation Spectroscopy." Accounts of Chemical Research 38, no. 6 (2005): 469–77. http://dx.doi.org/10.1021/ar040026l.

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Korlach, J., P. Schwille, W. W. Webb, and G. W. Feigenson. "Characterization of lipid bilayer phases by confocal microscopy and fluorescence correlation spectroscopy." Proceedings of the National Academy of Sciences 96, no. 15 (1999): 8461–66. http://dx.doi.org/10.1073/pnas.96.15.8461.

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Kudrat-E-Zahan, Md, Salih Zeki Yildiz, and Senem Colak Yazici. "Preparation and Characterization of Highly Fluorescent TGA-CdTe Quantum Dot-Hyamine 1622 Additive Composite." Micro and Nanosystems 12, no. 2 (2020): 92–101. http://dx.doi.org/10.2174/1876402912666200225111411.

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Objective: The aim of the present study was to prepare highly luminescent additive composite polymer with hyamine 1622 and Thioglycolic Acid (TGA) coated CdTe Quantum Dots (QDs). Methods: The additive nano-composite was synthesized by the colloid synthesis method for the first time. The properties like particle size, fluorescence efficiency, fluorescence imaging, self-assembling, quantum dots, encapsulation, etc. were characterized by the employing of instrumental techniques such as 1H and 13C NMR, Fourier Transform Infrared spectroscopy (FT-IR), BAB image analysis system spectroscopy, Atomic

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Nie, Shuming, Daniel T. Chiu, and Richard N. Zare. "Real-time observation of single molecules by confocal fluorescence microscopy." Proceedings, annual meeting, Electron Microscopy Society of America 53 (August 13, 1995): 60–61. http://dx.doi.org/10.1017/s0424820100136672.

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The ability to detect, identify, and manipulate individual molecules offer exciting possibilities in many fields, including chemical analysis, materials research, and the biological sciences. A particularly powerful approach is to combine the exquisite sensitivity of laser-induced fluorescence and the spatial localization and imaging capabilities of diffraction-limited or near-field optical microscopes. Unlike scanning tunneling microscopy (STM) and atomic force microscopy (AFM), which lack molecular specificity, optical spectroscopy and microscopy techniques can be used for real-time monitori

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Cowger, Win, Andrew Gray, Silke H. Christiansen, et al. "Critical Review of Processing and Classification Techniques for Images and Spectra in Microplastic Research." Applied Spectroscopy 74, no. 9 (2020): 989–1010. http://dx.doi.org/10.1177/0003702820929064.

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Microplastic research is a rapidly developing field, with urgent needs for high throughput and automated analysis techniques. We conducted a review covering image analysis from optical microscopy, scanning electron microscopy, fluorescence microscopy, and spectral analysis from Fourier transform infrared (FT-IR) spectroscopy, Raman spectroscopy, pyrolysis gas–chromatography mass–spectrometry, and energy dispersive X-ray spectroscopy. These techniques were commonly used to collect, process, and interpret data from microplastic samples. This review outlined and critiques current approaches for a

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Gupta, Anjali, Thomas Korte, Andreas Herrmann, and Thorsten Wohland. "Plasma membrane asymmetry of lipid organization: fluorescence lifetime microscopy and correlation spectroscopy analysis." Journal of Lipid Research 61, no. 2 (2019): 252–66. http://dx.doi.org/10.1194/jlr.d119000364.

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A fundamental feature of the eukaryotic cell membrane is the asymmetric arrangement of lipids in its two leaflets. A cell invests significant energy to maintain this asymmetry and uses it to regulate important biological processes, such as apoptosis and vesiculation. The dynamic coupling of the inner or cytoplasmic and outer or exofacial leaflets is a challenging open question in membrane biology. Here, we combined fluorescence lifetime imaging microscopy (FLIM) with imaging total internal reflection fluorescence correlation spectroscopy (ITIR-FCS) to differentiate the dynamics and organizatio

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Zhang, Zhimin, Shaocong Liu, Liang Xu, et al. "Super-resolution microscopy based on parallel detection." Journal of Innovative Optical Health Sciences 12, no. 06 (2019): 1950023. http://dx.doi.org/10.1142/s1793545819500238.

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Image scanning microscopy based on pixel reassignment can improve the confocal resolution limit without losing the image signal-to-noise ratio (SNR) greatly [C. J. R. Sheppard, “Super-resolution in confocal imaging,” Optik 80(2) 53–54 (1988). C. B. Müller, E. Jörg, “Image scanning microscopy, “Phys. Rev. Lett. 104(19) 198101 (2010). C. J. R. Sheppard, S. B. Mehta, R. Heintzmann, “Superresolution by image scanning microscopy using pixel reassignment,” Opt. Lett. 38(15) 2889–2892 (2013)]. Here, we use a tailor-made optical fiber and 19 avalanche photodiodes (APDs) as parallel detectors to upgrad

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Marriott, Gerard, Shu Mao, Tomoyo Sakata, et al. "Optical lock-in detection imaging microscopy for contrast-enhanced imaging in living cells." Proceedings of the National Academy of Sciences 105, no. 46 (2008): 17789–94. http://dx.doi.org/10.1073/pnas.0808882105.

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One of the limitations on imaging fluorescent proteins within living cells is that they are usually present in small numbers and need to be detected over a large background. We have developed the means to isolate specific fluorescence signals from background by using lock-in detection of the modulated fluorescence of a class of optical probe termed “optical switches.” This optical lock-in detection (OLID) approach involves modulating the fluorescence emission of the probe through deterministic, optical control of its fluorescent and nonfluorescent states, and subsequently applying a lock-in de

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Huang, Jiahao, Peter X. Chen, and Shawn Wettig. "Fluorescence-based techniques to assess the miscibility and physical stability of a drug–lipid complex." Canadian Journal of Chemistry 97, no. 6 (2019): 496–503. http://dx.doi.org/10.1139/cjc-2018-0404.

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The objective of this study was to evaluate the feasibility of using fluorescence-based techniques to assess the miscibility and physical stability of a drug–lipid complex pharmaceutical dosage form under a solvent-free condition. An indomethacin–phospholipid complex (IDM–DPC) was used as model complex for this study. The miscibility of indomethacin within the phospholipid was assessed by fluorescence spectroscopy, fluorescence microscopy, and infrared spectroscopy. The miscibility limit of the complex system was determined by fluorescence to be 20%–30% drug loading content, showing good corre

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