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Littérature scientifique sur le sujet « Genetic vectors »

Auteur : Grafiati

Publié le 4 juin 2021

Mis à jour le 25 juillet 2025

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Articles de revues sur le sujet "Genetic vectors"

Gooding, R. H. "Genetic variation in arthropod vectors of disease-causing organisms: obstacles and opportunities." Clinical Microbiology Reviews 9, no. 3 (1996): 301–20. http://dx.doi.org/10.1128/cmr.9.3.301.

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An overview of the genetic variation in arthropods that transmit pathogens to vertebrates is presented, emphasizing the genetics of vector-pathogen relationships and the biochemical genetics of vectors. Vector-pathogen interactions are reviewed briefly as a prelude to a discussion of the genetics of susceptibility and refractoriness in vectors. Susceptibility to pathogens is controlled by maternally inherited factors, sex-linked dominant alleles, and dominant and recessive autosomal genes. There is widespread interpopulation (including intercolony) and temporal variation in susceptibility to p

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Yew, Chee-Hong Takahiro, Narmatha Gurumoorthy, Fazlina Nordin, et al. "Integrase deficient lentiviral vector: prospects for safe clinical applications." PeerJ 10 (August 12, 2022): e13704. http://dx.doi.org/10.7717/peerj.13704.

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HIV-1 derived lentiviral vector is an efficient transporter for delivering desired genetic materials into the targeted cells among many viral vectors. Genetic material transduced by lentiviral vector is integrated into the cell genome to introduce new functions, repair defective cell metabolism, and stimulate certain cell functions. Various measures have been administered in different generations of lentiviral vector systems to reduce the vector’s replicating capabilities. Despite numerous demonstrations of an excellent safety profile of integrative lentiviral vectors, the precautionary approa

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Verma, Abhishek, and Ankit Awasthi. "Developing non-viral or viral vectors for efficient and targeted delivery of genetic material, such as DNA or RNA, for gene therapy applications." Pharmaspire 15, no. 04 (2023): 243–56. http://dx.doi.org/10.56933/pharmaspire.2023.15137.

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Gene therapy has emerged as a promising approach for treating a wide range of genetic and acquired diseases by introducing or modifying genetic material within cells. To achieve successful gene therapy, efficient and precise delivery of genetic material, such as DNA or RNA, to target cells is essential. This abstract explores the development of both non-viral and viral vectors for the delivery of genetic material in gene therapy applications. Non-viral vectors, including lipid nanoparticles (LNPs), polymer-based carriers, and cell-penetrating peptides, have gained significant attention due to

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Perumalsamy, Nandhini, Rohit Sharma, Muthukumaravel Subramanian, and Shriram Ananganallur Nagarajan. "Hard Ticks as Vectors: The Emerging Threat of Tick-Borne Diseases in India." Pathogens 13, no. 7 (2024): 556. http://dx.doi.org/10.3390/pathogens13070556.

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Hard ticks (Ixodidae) play a critical role in transmitting various tick-borne diseases (TBDs), posing significant global threats to human and animal health. Climatic factors influence the abundance, diversity, and vectorial capacity of tick vectors. It is imperative to have a comprehensive understanding of hard ticks, pathogens, eco-epidemiology, and the impact of climatic changes on the transmission dynamics of TBDs. The distribution and life cycle patterns of hard ticks are influenced by diverse ecological factors that, in turn, can be impacted by changes in climate, leading to the expansion

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Weklak, Denice, Daniel Pembaur, Georgia Koukou, Franziska Jönsson, Claudia Hagedorn, and Florian Kreppel. "Genetic and Chemical Capsid Modifications of Adenovirus Vectors to Modulate Vector–Host Interactions." Viruses 13, no. 7 (2021): 1300. http://dx.doi.org/10.3390/v13071300.

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Adenovirus-based vectors are playing an important role as efficacious genetic vaccines to fight the current COVID-19 pandemic. Furthermore, they have an enormous potential as oncolytic vectors for virotherapy and as vectors for classic gene therapy. However, numerous vector–host interactions on a cellular and noncellular level, including specific components of the immune system, must be modulated in order to generate safe and efficacious vectors for virotherapy or classic gene therapy. Importantly, the current widespread use of Ad vectors as vaccines against COVID-19 will induce antivector imm

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Powell, Jeffrey. "Genetic Variation in Insect Vectors: Death of Typology?" Insects 9, no. 4 (2018): 139. http://dx.doi.org/10.3390/insects9040139.

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The issue of typological versus population thinking in biology is briefly introduced and defined. It is then emphasized how population thinking is most relevant and useful in vector biology. Three points are made: (1) Vectors, as they exist in nature, are genetically very heterogeneous. (2) Four examples of how this is relevant in vector biology research are presented: Understanding variation in vector competence, GWAS, identifying the origin of new introductions of invasive species, and resistance to inbreeding. (3) The existence of high levels of vector genetic heterogeneity can lead to fail

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Kienesberger, Sabine, Gregor Gorkiewicz, Martina M. Joainig, Sylvia R. Scheicher, Eva Leitner, and Ellen L. Zechner. "Development of Experimental Genetic Tools for Campylobacter fetus." Applied and Environmental Microbiology 73, no. 14 (2007): 4619–30. http://dx.doi.org/10.1128/aem.02407-06.

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ABSTRACT Molecular analysis of the virulence mechanisms of the emerging pathogen Campylobacter fetus has been hampered by the lack of genetic tools. We report the development and functional analysis of Escherichia coli-Campylobacter shuttle vectors that are appropriate for C. fetus. Some vectors were constructed based on the known Campylobacter coli plasmid pIP1455 replicon, which confers a wide host range in Campylobacter spp. Versatility in directing gene expression was achieved by introducing a strong C. fetus promoter. The constructions carry features necessary and sufficient to detect the

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Nagoba, Shivappa N.* Khurde Sonali S. Shaikh Atiya L. Shimge Krishna R. "A REVIEW ON GENE THERAPY FOR THE GENETIC REVOLUTION." INDO AMERICAN JOURNAL OF PHARMACEUTICAL SCIENCES 05, no. 07 (2018): 7111–22. https://doi.org/10.5281/zenodo.1325916.

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<em>The ability to make site-specific modifications to the human genome has been an objective in medicine since the recognition of the gene as the basic unit of heredity. Thus, gene therapy is understood as the ability of genetic improvement through the correction of altered (mutated) genes or site-specific modifications that target therapeutic treatment. It is the concept of gene therapy involves the transfer of genetic material in to a cell, tissue, or whole organism to cure a disease and improve the Patient compliance. Gene therapy is to transform viruses into genetic material, which will d

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Criscione, Frank, David A. O’Brochta, and William Reid. "Genetic technologies for disease vectors." Current Opinion in Insect Science 10 (August 2015): 90–97. http://dx.doi.org/10.1016/j.cois.2015.04.012.

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Krasnykh, Victor N., Joanne T. Douglas, and Victor W. van Beusechem. "Genetic Targeting of Adenoviral Vectors." Molecular Therapy 1, no. 5 (2000): 391–405. http://dx.doi.org/10.1006/mthe.2000.0062.

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Thèses sur le sujet "Genetic vectors"

Theodorides, Kosmas. "Genetic and systematic studies on Cicadellidae vectors." Thesis, University of East Anglia, 2000. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.368187.

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Shareck, Julie. "Isolation and characterization of a cryptic plasmid from Lactobacillus plantarum." Thesis, McGill University, 2005. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=84072.

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Lactic acid bacteria (LAB), a group of generally recognized as safe (GRAS) organisms that metabolize sugars into primarily lactic acid, have traditionally been used for the fermentation and preservation of various foods and beverages. There is increasing interest in the genetic manipulation of LAB to improve existing characteristics or introduce novel, industrially pertinent phenotypes. However, because these bacteria have food-related applications, their genetic modification requires the use of food-grade genetic engineering tools. LAB plasmids, self-replicating extrachromosomal DNA mo

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Robson, Julia. "The construction of an expression vector for the transformation of the grape chloroplast genome." Thesis, Stellenbosch : Stellenbosch University, 2003. http://hdl.handle.net/10019.1/53621.

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Thesis (MSc)--University of Stellenbosch, 2003.<br>ENGLISH ABSTRACT: The genetic information of plants is found in the nucleus, the mitochondria, and the plastids. The DNA of plastids is comprised of multiple copies of a double-stranded, circular, prokaryoticallyderived genome of -150 kb. The genome equivalents of plastid organelles in higher plant cells are an attractive target for genetic engineering as high protein expression levels are readily obtained due to the high genome copy number per organelle. The resultant proteins are contained within the plastid organelle and the correspon

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Ghosh, Arkasubhra. "Rational design of split gene vectors to expand the packaging capacity of adeno-associated viral vectors." Diss., Columbia, Mo. : University of Missouri-Columbia, 2007. http://hdl.handle.net/10355/4712.

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Thesis (Ph. D.)--University of Missouri-Columbia, 2007.<br>The entire dissertation/thesis text is included in the research.pdf file; the official abstract appears in the short.pdf file (which also appears in the research.pdf); a non-technical general description, or public abstract, appears in the public.pdf file. Vita. "December 2007" Includes bibliographical references.

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Wood, David Rowe Ding Jiahuan. "Design, optimization, and evaluation of conditionally active gene therapy vectors." Waco, Tex. : Baylor University, 2008. http://hdl.handle.net/2104/5153.

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Mück-Häusl, Martin Andreas. "Genetic engineering of adenoviral vectors for improved therapeutic applications." Diss., lmu, 2011. http://nbn-resolving.de/urn:nbn:de:bvb:19-138269.

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Wong, Tik-wun Lina. "Construction of an infectious PRRSV cDNA clone and its use as a vector for foreign gene expression." Click to view the E-thesis via HKUTO, 2010. http://sunzi.lib.hku.hk/hkuto/record/B44251841.

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Van, Eeden C. (Christiaan). "The construction of gene silencing transformation vectors for the introduction of multiple-virus resistance in grapevines." Thesis, Stellenbosch : Stellenbosch University, 2004. http://hdl.handle.net/10019.1/53764.

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Thesis (MSc)--University of Stellenbosch, 2004.<br>ENGLISH ABSTRACT: Viruses are some of the most important pathogens of grapevines. There are no effective chemical treatments, and no grapevine- or other natural resistance genes have been discovered against grapevine infecting viruses. The primary method of grapevine virus control is prevention by biological indexing and molecular- and serological screening of rootstocks and scions before propagation. Due to the spread of grapevine viruses through insect vectors, and in the case of GRSPaV the absence of serological screening, these metho

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Warren, Ann. "Transposable genetic elements in the mosquito Aedes aegypti." Thesis, University of Liverpool, 1989. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.237672.

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Limberis, Maria. "A lentiviral gene transfer vector for the treatment of cystic fibrosis airway disease." Title page, synopsis and list of contents only, 2002. http://web4.library.adelaide.edu.au/theses/09PH/09phl735.pdf.

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"16th September 2002." Accompanying CD contains 2 MPEG clips with accompanying text, and a copy in PDF format of: Recovery of airway cystic fibrosis transmembrane conductance regulator function in mice with cystic fibrosis after single-dose lentivirus-mediated gene transfer / M. Limberis ... [et al.], published in Human gene therapy vol. 13 (2002). Bibliography: leaves xxix-li. This thesis focuses on modulating the physical barriers of the airway epithelium with mild detergents, so as to enhance gene transfer by a HIV-1 based lentivirus vector in vivo. The efficiency of the gene transfer was

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Livres sur le sujet "Genetic vectors"

L, Hefferon Kathleen, ed. Virus expression vectors. Transworld Research Network, 2007.

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S, Ruiz Pablo, ed. Genetic vectors research focus. Nova Biomedical Books, 2007.

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David, Curiel, and Douglas Joanne T, eds. Adenoviral vectors for gene therapy. Academic Press, 2002.

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George, Dickson, ed. Molecular and cell biology of human gene therapeutics. Chapman & Hall, 1995.

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Yakov, Gluzman, Hughes Stephen H, and Cold Spring Harbor Laboratory, eds. Viral vectors. Cold Spring Harbor Laboratory, 1988.

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L, Rodriguez Raymond, and Denhardt David T, eds. Vectors: A survey of molecular cloning vectors and their uses. Butterworths, 1988.

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D, Richardson Christopher, ed. Baculovirus expression protocols. Humana Press, 1995.

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H, Miller Jeffrey, and Calos Michele P, eds. Gene transfer vectors for mammalian cells. Cold Spring Harbor Laboratory, 1987.

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P, Jones, ed. Vectors: Cloning applications. Wiley, 1998.

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P, Jones, ed. Vectors: Expression systems. John Wiley & Sons, 1998.

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Chapitres de livres sur le sujet "Genetic vectors"

Benabdellah, Karim, Simone Thomas, and Hinrich Abken. "Genetic Engineering of Autologous or Allogeneic Immune Effector Cells." In The EBMT/EHA CAR-T Cell Handbook. Springer International Publishing, 2022. http://dx.doi.org/10.1007/978-3-030-94353-0_2.

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AbstractManufacturing immune effector cells (T or NK cells) with CAR-encoding DNA sequences requires efficient and safe genetic engineering procedures. For this purpose, an appropriate genetic vector is chosen according to numerous factors, including the vector genome packaging capacity, cellular tropism, genomic integration, immune toxicity, and other factors. In clinical trials, genomes integrating viral vectors, in particular vectors based on members of the Retroviridae family, such as retroviruses and lentiviruses, have been successfully used for more than 20 years. These vectors contain a

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Vos, Jean-Michel H. "Herpesviruses as Genetic Vectors." In Viruses in Human Gene Therapy. Springer Netherlands, 1995. http://dx.doi.org/10.1007/978-94-011-0555-2_5.

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Cox, William I., Russell R. Gettig, and Enzo Paoletti. "Poxviruses as Genetic Vectors." In Viruses in Human Gene Therapy. Springer Netherlands, 1995. http://dx.doi.org/10.1007/978-94-011-0555-2_6.

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Gulhan, Baris. "Biological Methods Used in Gene Therapy." In Gene Therapy. Nobel Tip Kitabevleri, 2024. http://dx.doi.org/10.69860/nobel.9786053358824.3.

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Gene therapy is used to prevent or treat genetic diseases. To treat genetic diseases, transfer of genes that correct the effects of the mutation that causes the disease to the patient. In gene therapy, the therapeutic gene is transferred to target cells through a vector and viral vectors are most used. Retroviruses (lentiviruses), adenoviruses, adeno-associated viruses, and herpes virus vectors are common as viral vectors. In this section, biological methods used especially in gene therapy stages examined.

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Bevan, Michael, and Andrew Goldsbrough. "Design and Use of Agrobacterium Transformation Vectors." In Genetic Engineering. Springer US, 1988. http://dx.doi.org/10.1007/978-1-4615-7081-3_7.

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Shepherd, Robert J. "Caulimoviruses as Potential Gene Vectors for Higher Plants." In Genetic Engineering. Springer US, 1986. http://dx.doi.org/10.1007/978-1-4615-9456-7_13.

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Dingli, David, and Stephen J. Russell. "Genetic Targeting of Retroviral Vectors." In Vector Targeting for Therapeutic Gene Delivery. John Wiley & Sons, Inc., 2003. http://dx.doi.org/10.1002/0471234303.ch13.

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Wickham, Thomas J. "Genetic Targeting of Adenoviral Vectors." In Vector Targeting for Therapeutic Gene Delivery. John Wiley & Sons, Inc., 2003. http://dx.doi.org/10.1002/0471234303.ch7.

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Bouyer, Jérémy, and Eric Marois. "14. Genetic control of vectors." In Ecology and Control of Vector-borne Diseases. Wageningen Academic Publishers, 2018. http://dx.doi.org/10.3920/978-90-8686-863-6_14.

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Nicklin, Stuart A., and Andrew H. Baker. "Development of Targeted Viral Vectors for Cardiovascular Gene Therapy." In Genetic Engineering. Springer US, 2003. http://dx.doi.org/10.1007/978-1-4615-0073-5_2.

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Actes de conférences sur le sujet "Genetic vectors"

Alshahrani, Mohammed, Spyridon Samothrakis, and Maria Fasli. "Identifying idealised vectors for emotion detection using CMA-ES." In GECCO '19: Genetic and Evolutionary Computation Conference. ACM, 2019. http://dx.doi.org/10.1145/3319619.3322057.

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Funaki, Ryohei, and Hideyuki Takagi. "Application of Gravity Vectors and Moving Vectors for the Acceleration of Both Differential Evolution and Interactive Differential Evolution." In 2011 Fifth International Conference on Genetic and Evolutionary Computing (ICGEC). IEEE, 2011. http://dx.doi.org/10.1109/icgec.2011.71.

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Madeira, Catarina, Sofia C. Ribeiro, Rui Mendes, Irina S. M. Pinheiro, Claudia L. da Silva, and Joaquim M. S. Cabral. "Genetic engineering of stem cells by non-viral vectors." In 2011 1st Portuguese Meeting in Bioengineering ¿ The Challenge of the XXI Century (ENBENG). IEEE, 2011. http://dx.doi.org/10.1109/enbeng.2011.6026044.

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Pescador-Rojas, Miriam, and Carlos A. Coello Coello. "Studying the effect of techniques to generate reference vectors in many-objective optimization." In GECCO '18: Genetic and Evolutionary Computation Conference. ACM, 2018. http://dx.doi.org/10.1145/3205651.3205684.

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Xianneng Li, S. Mabu, M. K. Mainali, and K. Hirasawa. "Probabilistic model building Genetic Network Programming using multiple probability vectors." In 2010 IEEE Region 10 Conference (TENCON 2010). IEEE, 2010. http://dx.doi.org/10.1109/tencon.2010.5686113.

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Fengli Sun, Quanhua Gao, and Jinguo Wang. "Ridgelet Probabilistic Neural Network with Genetic Algorithm selecting center vectors." In 2011 International Conference on Computer Science and Network Technology (ICCSNT). IEEE, 2011. http://dx.doi.org/10.1109/iccsnt.2011.6182206.

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Sato, Yuji, Mads Midtlyng, and Mikiko Sato. "Diffusely Distributed Parallelization of MOEA/D with Edge Weight Vectors Sharing." In GECCO '23 Companion: Companion Conference on Genetic and Evolutionary Computation. ACM, 2023. http://dx.doi.org/10.1145/3583133.3590738.

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Tanaka, Mariko, Yuki Yamagishi, Hidetoshi Nagai, and Hiroyuki Sato. "Infeasible solution repair and MOEA/D sharing weight vectors for solving multi-objective set packing problems." In GECCO '18: Genetic and Evolutionary Computation Conference. ACM, 2018. http://dx.doi.org/10.1145/3205651.3208765.

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Sánchez, Alberto Rodríguez, Antonin Ponsich, and Antonio López Jaimes. "Generation techniques and a novel on-line adaptation strategy for weight vectors within decomposition-based MOEAs." In GECCO '19: Genetic and Evolutionary Computation Conference. ACM, 2019. http://dx.doi.org/10.1145/3319619.3322055.

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Xie, Yonghua, Lokesh Setia, and Hans Burkhardt. "Block DCT Vectors Construction for Face Retrieval Based on Genetic Algorithm." In Third International Conference on Natural Computation (ICNC 2007). IEEE, 2007. http://dx.doi.org/10.1109/icnc.2007.286.

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Rapports d'organisations sur le sujet "Genetic vectors"

Dawson, William O., and Moshe Bar-Joseph. Creating an Ally from an Adversary: Genetic Manipulation of Citrus Tristeza. United States Department of Agriculture, 2004. http://dx.doi.org/10.32747/2004.7586540.bard.

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Citrus is one of the major agricultural crops common to Israel and the United States, important in terms of nutrition, foreign exchange, and employment. The economy of both citrus industries have been chronically plagued by diseases caused by Citrus tristeza virus (CTV). The short term solution until virus-resistant plants can be used is the use of mild strain cross-protection. We are custom designing "ideal" protecting viruses to immunize trees against severe isolates of CTV by purposely inoculating existing endangered trees and new plantings to be propagated as infected (protected) citrus bu

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Liu, Zhanjiang John, Rex Dunham, and Boaz Moav. Developmental and Evaluation of Advanced Expression Vectors with Both Enhanced Integration and Stable Expression for Transgenic Farmed Fish. United States Department of Agriculture, 2001. http://dx.doi.org/10.32747/2001.7585196.bard.

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The objectives of the project were to develop expression vectors using the Sleeping Beauty transposon technology and the genetic border elements to provide both enhanced integration rate and stable transgene expression, and to evaluate the application of such vectors in farmed fish such as catfish and carp. The panel recommended adding the objective of evaluating the endogenous transposable elements, particularly in catfish, in order to evaluate the applicability of the expression vectors while reduc1ng efforts in real production of transgenic fish considering the focus of the project was to d

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Jung, Carina, Karl Indest, Matthew Carr, Richard Lance, Lyndsay Carrigee, and Kayla Clark. Properties and detectability of rogue synthetic biology (SynBio) products in complex matrices. Engineer Research and Development Center (U.S.), 2022. http://dx.doi.org/10.21079/11681/45345.

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Synthetic biology (SynBio) aims to rationally engineer or modify traits of an organism or integrate the behaviors of multiple organisms into a singular functional organism through advanced genetic engineering techniques. One objective of this research was to determine the environmental persistence of engineered DNA in the environment. To accomplish this goal, the environmental persistence of legacy engineered DNA building blocks were targeted that laid the foundation for SynBio product development and application giving rise to “post-use products.” These building blocks include genetic constru

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Perl, Avichai, Bruce I. Reisch, and Ofra Lotan. Transgenic Endochitinase Producing Grapevine for the Improvement of Resistance to Powdery Mildew (Uncinula necator). United States Department of Agriculture, 1994. http://dx.doi.org/10.32747/1994.7568766.bard.

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The original objectives are listed below: 1. Design vectors for constitutive expression of endochitinase from Trichoderma harzianum strain P1. Design vectors with signal peptides to target gene expression. 2. Extend transformation/regeneration technology to other cultivars of importance in the U.S. and Israel. 3. Transform cultivars with the endochitinase constructs developed as part of objective 1. A. Characterize foliar powdery mildew resistance in transgenic plants. Background of the topic Conventional breeding of grapevines is a slow and imprecise process. The long generation cycle, large

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Jougwutiwes, Somchai, Chaturong Putaporntip, and Jeeraphat Sirichaisinthop. Plasmodium knowlesi, an emerging human malaria : molecular surveillance, genetic characterization and role of anopheline malaria main vectors in Thailand in disease transmission. Chulalongkorn University, 2011. https://doi.org/10.58837/chula.res.2011.26.

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Naturally acquired human infections with Plasmodium knowlesi are endemic in Southeast Asia. Our previous survey in 2006-2007 has shown a wide-spread and low prevalence of this simian malaria in Thai patients. This follow-up study in the same endemic areas in 2008-2009 has revealed a stable prevalence of P. knowlesi among malaria patients whereas a significance difference in the prevalence of 4 human malaria species occurred. Retrospective analysis of blood samples from malaria patients collected in 1996 in one of these endemic areas has reaffirmed a stable prevalence of P.knowlesi when compare

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Ullman, Diane, James Moyer, Benjamin Raccah, Abed Gera, Meir Klein, and Jacob Cohen. Tospoviruses Infecting Bulb Crops: Evolution, Diversity, Vector Specificity and Control. United States Department of Agriculture, 2002. http://dx.doi.org/10.32747/2002.7695847.bard.

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Objectives. The overall goal of the proposed research was to develop a mechanistic understanding of tospovirus evolution, diversity and vector specificity that could be applied to development of novel methods for limiting virus establishment and spread. Our specific objectives were: 1) To characterize newly intercepted tospoviruses in onion, Hippeastrum and other bulb crops and compare them with the known tomato spotted wilt virus (TSWV) and its isolates; 2) To characterize intra- and interspecific variation in the virus transmission by thrips of the new and distinct tospoviruses. and, 3) To d

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Singh, Anjali. What Is Optogenetics and How Does It Work? ConductScience, 2022. http://dx.doi.org/10.55157/cs20220704.

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Optogenetics is a biotechnological method that combines optical systems and genetic engineering to control and monitor the functions of cells, tissues, and organisms. It involves using light-sensitive proteins called opsins to manipulate specific cells or regions with precision. This technique has revolutionized neuroscience, allowing researchers to study neural circuits and behavior by turning cells on and off. Opsins are categorized into microbial and animal types, each with specific functions. Optogenetic experiments require opsins, suitable plasmids or viral vectors, and a light source. Th

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Mawassi, Munir, Baozhong Meng, and Lorne Stobbs. Development of Virus Induced Gene Silencing Tools for Functional Genomics in Grapevine. United States Department of Agriculture, 2013. http://dx.doi.org/10.32747/2013.7613887.bard.

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Grapevine is perhaps the most widely grown fruit crop. To understand the genetic make-up so as to improve the yield and quality of grapes and grape products, researchers in Europe have recently sequenced the genomes of Pinot noir and its inbred. As expected, function of many grape genes is unknown. Functional genomics studies have become the major focus of grape researchers and breeders. Current genetic approaches for gene function studies include mutagenesis, crossing and genetic transformation. However, these approaches are difficult to apply to grapes and takes long periods of time to accom

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Mawassi, Munir, and Valerian V. Dolja. Role of the viral AlkB homologs in RNA repair. United States Department of Agriculture, 2014. http://dx.doi.org/10.32747/2014.7594396.bard.

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AlkB proteins that repair DNA via reversing methylation damage are conserved in a broad range of prokaryotes and eukaryotes including plants. Surprisingly, AlkB-domains were discovered in the genomes of numerous plant positive-strand RNA viruses, majority of which belong to the family Flexiviridae. The major goal of this research was to reveal the AlkB functions in the viral infection cycle using a range of complementary genetic and biochemical approaches. Our hypotheses was that AlkB is required for efficient replication and genetic stability of viral RNA genomes The major objectives of the r

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Bar-Joseph, Moshe, William O. Dawson, and Munir Mawassi. Role of Defective RNAs in Citrus Tristeza Virus Diseases. United States Department of Agriculture, 2000. http://dx.doi.org/10.32747/2000.7575279.bard.

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This program focused on citrus tristeza virus (CTV), the largest and one of the most complex RNA-plant-viruses. The economic importance of this virus to the US and Israeli citrus industries, its uniqueness among RNA viruses and the possibility to tame the virus and eventually turn it into a useful tool for the protection and genetic improvement of citrus trees justify these continued efforts. Although the overall goal of this project was to study the role(s) of CTV associated defective (d)-RNAs in CTV-induced diseases, considerable research efforts had to be devoted to the engineering of the h

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