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Articles de revues sur le sujet « Genetic vectors »

Auteur : Grafiati

Publié le 4 juin 2021

Mis à jour le 25 juillet 2025

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1

Gooding, R. H. "Genetic variation in arthropod vectors of disease-causing organisms: obstacles and opportunities." Clinical Microbiology Reviews 9, no. 3 (1996): 301–20. http://dx.doi.org/10.1128/cmr.9.3.301.

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An overview of the genetic variation in arthropods that transmit pathogens to vertebrates is presented, emphasizing the genetics of vector-pathogen relationships and the biochemical genetics of vectors. Vector-pathogen interactions are reviewed briefly as a prelude to a discussion of the genetics of susceptibility and refractoriness in vectors. Susceptibility to pathogens is controlled by maternally inherited factors, sex-linked dominant alleles, and dominant and recessive autosomal genes. There is widespread interpopulation (including intercolony) and temporal variation in susceptibility to p

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Yew, Chee-Hong Takahiro, Narmatha Gurumoorthy, Fazlina Nordin, et al. "Integrase deficient lentiviral vector: prospects for safe clinical applications." PeerJ 10 (August 12, 2022): e13704. http://dx.doi.org/10.7717/peerj.13704.

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HIV-1 derived lentiviral vector is an efficient transporter for delivering desired genetic materials into the targeted cells among many viral vectors. Genetic material transduced by lentiviral vector is integrated into the cell genome to introduce new functions, repair defective cell metabolism, and stimulate certain cell functions. Various measures have been administered in different generations of lentiviral vector systems to reduce the vector’s replicating capabilities. Despite numerous demonstrations of an excellent safety profile of integrative lentiviral vectors, the precautionary approa

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Verma, Abhishek, and Ankit Awasthi. "Developing non-viral or viral vectors for efficient and targeted delivery of genetic material, such as DNA or RNA, for gene therapy applications." Pharmaspire 15, no. 04 (2023): 243–56. http://dx.doi.org/10.56933/pharmaspire.2023.15137.

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Gene therapy has emerged as a promising approach for treating a wide range of genetic and acquired diseases by introducing or modifying genetic material within cells. To achieve successful gene therapy, efficient and precise delivery of genetic material, such as DNA or RNA, to target cells is essential. This abstract explores the development of both non-viral and viral vectors for the delivery of genetic material in gene therapy applications. Non-viral vectors, including lipid nanoparticles (LNPs), polymer-based carriers, and cell-penetrating peptides, have gained significant attention due to

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Perumalsamy, Nandhini, Rohit Sharma, Muthukumaravel Subramanian, and Shriram Ananganallur Nagarajan. "Hard Ticks as Vectors: The Emerging Threat of Tick-Borne Diseases in India." Pathogens 13, no. 7 (2024): 556. http://dx.doi.org/10.3390/pathogens13070556.

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Hard ticks (Ixodidae) play a critical role in transmitting various tick-borne diseases (TBDs), posing significant global threats to human and animal health. Climatic factors influence the abundance, diversity, and vectorial capacity of tick vectors. It is imperative to have a comprehensive understanding of hard ticks, pathogens, eco-epidemiology, and the impact of climatic changes on the transmission dynamics of TBDs. The distribution and life cycle patterns of hard ticks are influenced by diverse ecological factors that, in turn, can be impacted by changes in climate, leading to the expansion

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Weklak, Denice, Daniel Pembaur, Georgia Koukou, Franziska Jönsson, Claudia Hagedorn, and Florian Kreppel. "Genetic and Chemical Capsid Modifications of Adenovirus Vectors to Modulate Vector–Host Interactions." Viruses 13, no. 7 (2021): 1300. http://dx.doi.org/10.3390/v13071300.

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Adenovirus-based vectors are playing an important role as efficacious genetic vaccines to fight the current COVID-19 pandemic. Furthermore, they have an enormous potential as oncolytic vectors for virotherapy and as vectors for classic gene therapy. However, numerous vector–host interactions on a cellular and noncellular level, including specific components of the immune system, must be modulated in order to generate safe and efficacious vectors for virotherapy or classic gene therapy. Importantly, the current widespread use of Ad vectors as vaccines against COVID-19 will induce antivector imm

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Powell, Jeffrey. "Genetic Variation in Insect Vectors: Death of Typology?" Insects 9, no. 4 (2018): 139. http://dx.doi.org/10.3390/insects9040139.

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The issue of typological versus population thinking in biology is briefly introduced and defined. It is then emphasized how population thinking is most relevant and useful in vector biology. Three points are made: (1) Vectors, as they exist in nature, are genetically very heterogeneous. (2) Four examples of how this is relevant in vector biology research are presented: Understanding variation in vector competence, GWAS, identifying the origin of new introductions of invasive species, and resistance to inbreeding. (3) The existence of high levels of vector genetic heterogeneity can lead to fail

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Kienesberger, Sabine, Gregor Gorkiewicz, Martina M. Joainig, Sylvia R. Scheicher, Eva Leitner, and Ellen L. Zechner. "Development of Experimental Genetic Tools for Campylobacter fetus." Applied and Environmental Microbiology 73, no. 14 (2007): 4619–30. http://dx.doi.org/10.1128/aem.02407-06.

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ABSTRACT Molecular analysis of the virulence mechanisms of the emerging pathogen Campylobacter fetus has been hampered by the lack of genetic tools. We report the development and functional analysis of Escherichia coli-Campylobacter shuttle vectors that are appropriate for C. fetus. Some vectors were constructed based on the known Campylobacter coli plasmid pIP1455 replicon, which confers a wide host range in Campylobacter spp. Versatility in directing gene expression was achieved by introducing a strong C. fetus promoter. The constructions carry features necessary and sufficient to detect the

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Nagoba, Shivappa N.* Khurde Sonali S. Shaikh Atiya L. Shimge Krishna R. "A REVIEW ON GENE THERAPY FOR THE GENETIC REVOLUTION." INDO AMERICAN JOURNAL OF PHARMACEUTICAL SCIENCES 05, no. 07 (2018): 7111–22. https://doi.org/10.5281/zenodo.1325916.

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<em>The ability to make site-specific modifications to the human genome has been an objective in medicine since the recognition of the gene as the basic unit of heredity. Thus, gene therapy is understood as the ability of genetic improvement through the correction of altered (mutated) genes or site-specific modifications that target therapeutic treatment. It is the concept of gene therapy involves the transfer of genetic material in to a cell, tissue, or whole organism to cure a disease and improve the Patient compliance. Gene therapy is to transform viruses into genetic material, which will d

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Criscione, Frank, David A. O’Brochta, and William Reid. "Genetic technologies for disease vectors." Current Opinion in Insect Science 10 (August 2015): 90–97. http://dx.doi.org/10.1016/j.cois.2015.04.012.

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Krasnykh, Victor N., Joanne T. Douglas, and Victor W. van Beusechem. "Genetic Targeting of Adenoviral Vectors." Molecular Therapy 1, no. 5 (2000): 391–405. http://dx.doi.org/10.1006/mthe.2000.0062.

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Kojic, Milorad, and William K. Holloman. "Shuttle vectors for genetic manipulations in Ustilago maydis." Canadian Journal of Microbiology 46, no. 4 (2000): 333–38. http://dx.doi.org/10.1139/w00-002.

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Shuttle vectors with new or improved features were constructed to enable facile genetic manipulations in the plant pathogen Ustilago maydis. Sets of plasmids selectable in media containing geneticin, carboxin, nourseothricin, or hygromycin, able to replicate autonomously, to transform U. maydis by integration, and to express foreign genes under control of the homologous glyceraldehyde-3-phosphate dehydrogenase promoter, were built upon a common pUC19 vector backbone. This permits a large number of choices for a cloning site, blue/white screening for recombinant plasmids, rapid transfer of a cl

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Redder, Peter, and Patrick Linder. "New Range of Vectors with a Stringent 5-Fluoroorotic Acid-Based Counterselection System for Generating Mutants by Allelic Replacement in Staphylococcus aureus." Applied and Environmental Microbiology 78, no. 11 (2012): 3846–54. http://dx.doi.org/10.1128/aem.00202-12.

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ABSTRACTWe have developed a range of vectors for allelic replacements inStaphylococcus aureusto facilitate genetic work in this opportunistic pathogen. The central feature of the vector range is a selection/counterselection system that takes advantage of the 5-fluoroorotic acid (FOA) resistance and pyrimidine prototrophy caused by the loss and gain, respectively, of thepyrFandpyrEgenes. This system allows for stringent counterselection of the vectors during the second homologous recombination of a classic allelic replacement. The basic vector pRLY2, which contains thepyrFEgenes fromBacillus su

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Cody, Will B., and Herman B. Scholthof. "Plant Virus Vectors 3.0: Transitioning into Synthetic Genomics." Annual Review of Phytopathology 57, no. 1 (2019): 211–30. http://dx.doi.org/10.1146/annurev-phyto-082718-100301.

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Plant viruses were first implemented as heterologous gene expression vectors more than three decades ago. Since then, the methodology for their use has varied, but we propose it was the merging of technologies with virology tools, which occurred in three defined steps discussed here, that has driven viral vector applications to date. The first was the advent of molecular biology and reverse genetics, which enabled the cloning and manipulation of viral genomes to express genes of interest (vectors 1.0). The second stems from the discovery of RNA silencing and the development of high-throughput

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Zouache, Karima, Albin Fontaine, Anubis Vega-Rua, et al. "Three-way interactions between mosquito population, viral strain and temperature underlying chikungunya virus transmission potential." Proceedings of the Royal Society B: Biological Sciences 281, no. 1792 (2014): 20141078. http://dx.doi.org/10.1098/rspb.2014.1078.

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Interactions between pathogens and their insect vectors in nature are under the control of both genetic and non-genetic factors, yet most studies on mosquito vector competence for human pathogens are conducted in laboratory systems that do not consider genetic and/or environmental variability. Evaluating the risk of emergence of arthropod-borne viruses (arboviruses) of public health importance such as chikungunya virus (CHIKV) requires a more realistic appraisal of genetic and environmental contributions to vector competence. In particular, sources of variation do not necessarily act independe

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NIJHOF, A. M. "Genetic make-up of arthropod vectors." Revue Scientifique et Technique de l'OIE 34, no. 1 (2015): 113–22. http://dx.doi.org/10.20506/rst.34.1.2348.

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Carlson, J., K. Olson, S. Higgs, and B. Beaty. "Molecular Genetic Manipulation of Mosquito Vectors." Annual Review of Entomology 40, no. 1 (1995): 359–88. http://dx.doi.org/10.1146/annurev.en.40.010195.002043.

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Lee, Joon-Yong, Min-Soeng Kim, and Ju-Jang Lee. "Compact Genetic Algorithms using belief vectors." Applied Soft Computing 11, no. 4 (2011): 3385–401. http://dx.doi.org/10.1016/j.asoc.2011.01.010.

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Ponnazhagan, Selvarangan. "Adenoassociated Virus Vectors for Genetic Immunization." Immunologic Research 26, no. 1-3 (2002): 247–54. http://dx.doi.org/10.1385/ir:26:1-3:247.

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Li, Shu-fen, Hua-lin Wang, Zhi-hong Hu, and Fei Deng. "Genetic modification of baculovirus expression vectors." Virologica Sinica 27, no. 2 (2012): 71–82. http://dx.doi.org/10.1007/s12250-012-3236-y.

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Bogoslovskaya, E. V., D. V. Glazkova, G. A. Shipulin, and V. V. Pokrovskii. "SAFETY OF RETROVIRAL VECTORS IN GENE THERAPY." Annals of the Russian academy of medical sciences 67, no. 10 (2012): 55–61. http://dx.doi.org/10.15690/vramn.v67i10.417.

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Retroviral vectors are widely used in gene therapy and found to be an effective tool for the delivery of genetic constructs into cells. A unique feature of these vectors is the ability to incorporate therapeutic genes into a chromosome that ensures its passage to all progeny cells and enables to cure the diseases requiring genetic correction of dividing cells such as hematopoietic cells or skin cells. Retroviral vectors have been successfully used in gene therapy clinical trials for the treatment of 2 forms of severe combined immunodeficiencies and some other hereditary blood disorders. Howeve

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Xiao, Cheng Yong, Bo Qiang Shi, Zhi Jun Hao, and Shuang Ming Zhu. "Gear Incipient Diagnosing Based on EEMD and Genetic-Support Vector Machine." Applied Mechanics and Materials 397-400 (September 2013): 2104–10. http://dx.doi.org/10.4028/www.scientific.net/amm.397-400.2104.

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Due to the incipient fault attributes of gear are not obvious, So a hybrid diagnosis model to gear diagnosing based on Ensemble Empirical Mode Decomposition (EEMD) and Genetic-Support Vector Machine (GSVM) was proposed. With the method of EEMD,the gear vibration signals are adaptively decomposed into a finite number of Intrinsic Mode Function (IMF),which can alleviate model mixing that may appear in conventional EMD method. It calculates the energy character vectors of every IMF components,energy feature extracted from a number of IMFs that contained the most dominant fault information could s

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Wang, Xutong, Mandi Wang, Jian Sun, Xiaolei Qu, Shixin Wang, and Tingting Sun. "Establishment of an Efficient Genetic Transformation System in Sanghuangporus baumii." Journal of Fungi 10, no. 2 (2024): 137. http://dx.doi.org/10.3390/jof10020137.

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(1) Background: Sanghuangporus baumii, a valuable medicinal fungus, has limited studies on its gene function due to the lack of a genetic transformation system. (2) Methods: This study aimed to establish an efficient Agrobacterium tumefaciens-mediated transformation (ATMT) system for S. baumii. This study involved cloning the promoter (glyceraldehyde-3-phosphate dehydrogenase, gpd) of S. baumii, reconstructing the transformation vector, optimizing the treatment of receptor tissues, and inventing a new method for screening positive transformants. (3) Results: The established ATMT system involve

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Bourgade, Barbara, James Millard, Christopher M. Humphreys, Nigel P. Minton, and M. Ahsanul Islam. "Enabling Ethanologenesis in Moorella thermoacetica through Construction of a Replicating Shuttle Vector." Fermentation 8, no. 11 (2022): 585. http://dx.doi.org/10.3390/fermentation8110585.

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Replicating plasmid shuttle vectors are key tools for efficient genetic and metabolic engineering applications, allowing the development of sustainable bioprocesses using non-model organisms with unique metabolic capabilities. To date, very limited genetic manipulation has been achieved in the thermophilic acetogen, Moorella thermoacetica, partly due to the lack of suitable shuttle vectors. However, M. thermoacetica has considerable potential as an industrial chassis organism, which can only be unlocked if reliable and effective genetic tools are in place. This study reports the construction o

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Sedova, E. S., D. N. Shcherbinin, A. I. Migunov, et al. "Recombinant Influenza Vaccines." Acta Naturae 4, no. 4 (2012): 17–27. http://dx.doi.org/10.32607/20758251-2012-4-4-17-27.

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This review covers the problems encountered in the construction and production of new recombinant influenza vaccines. New approaches to the development of influenza vaccines are investigated; they include reverse genetics methods, production of virus-like particles, and DNA- and viral vector-based vaccines. Such approaches as the delivery of foreign genes by DNA- and viral vector-based vaccines can preserve the native structure of antigens. Adenoviral vectors are a promising gene-delivery platform for a variety of genetic vaccines. Adenoviruses can efficiently penetrate the human organism thro

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Moon, Seung-Hyun, and Yourim Yoon. "Genetic Mean Reversion Strategy for Online Portfolio Selection with Transaction Costs." Mathematics 10, no. 7 (2022): 1073. http://dx.doi.org/10.3390/math10071073.

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Online portfolio selection (OLPS) is a procedure for allocating portfolio assets using only past information to maximize an expected return. There have been successful mean reversion strategies that have achieved large excess returns on the traditional OLPS benchmark datasets. We propose a genetic mean reversion strategy that evolves a population of portfolio vectors using a hybrid genetic algorithm. Each vector represents the proportion of the portfolio assets, and our strategy chooses the best vector in terms of the expected returns on every trading day. To test our strategy, we used the pri

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CHEN, PENG, CHUNMEI LIU, LEGAND BURGE, MOHAMMAD MAHMOOD, WILLIAM SOUTHERLAND, and CLAY GLOSTER. "PROTEIN FOLD CLASSIFICATION WITH GENETIC ALGORITHMS AND FEATURE SELECTION." Journal of Bioinformatics and Computational Biology 07, no. 05 (2009): 773–88. http://dx.doi.org/10.1142/s0219720009004321.

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Protein fold classification is a key step to predicting protein tertiary structures. This paper proposes a novel approach based on genetic algorithms and feature selection to classifying protein folds. Our dataset is divided into a training dataset and a test dataset. Each individual for the genetic algorithms represents a selection function of the feature vectors of the training dataset. A support vector machine is applied to each individual to evaluate the fitness value (fold classification rate) of each individual. The aim of the genetic algorithms is to search for the best individual that

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Corbin, Seana, Lauren Johnson, Ana Azevedo-Pouly, Marie Burdine, and Lyle Burdine. "The use of DNAPK inhibitors increases the efficacy of Lentiviral vectors and decreases off-target effects." Journal of Immunology 212, no. 1_Supplement (2024): 1518_5519. http://dx.doi.org/10.4049/jimmunol.212.supp.1518.5519.

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Abstract Lentiviral vectors are a powerful tool in many models to deliver genetic material to target cells. Many methods of developing gene therapy treatment for multiple human diseases rely on using viral vectors to deliver genetic material into target cells. While viral vectors are a powerful tool, their use is not without its limitations and hurdles. Work must be done to overcome these hurdles to improve the delivery and reliability of vector-based gene therapy mechanisms in a clinically relevant and affordable manner. The primary objective of this study was to enhance the precision and eff

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Moskalev, A. V., B. Yu Gumilevskiy, A. V. Apchel, and V. N. Tsygan. "Genetic updating and marks of cellular lines." Bulletin of the Russian Military Medical Academy 22, no. 3 (2020): 168–75. http://dx.doi.org/10.17816/brmma50555.

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Abstract. Despite great advances in the biology of stem cells, there are still many dark spots. Genetic modification techniques, which can be used to track the lines of different cells, primarily stem cells, help to solve this problem. Various methods of biotechnology research are considered, allowing to evaluate the options of introducing new genes into cells and even whole organisms, as well as methods of controlling their expression in time and space, their activation, differentiation and decrease in functional activity, expression of several target genes. Options with multi-cystron vectors

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Yildirim, Özal, and Ulas Baran Baloglu. "Heartbeat type classification with optimized feature vectors." An International Journal of Optimization and Control: Theories & Applications (IJOCTA) 8, no. 2 (2018): 170–75. http://dx.doi.org/10.11121/ijocta.01.2018.00567.

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In this study, a feature vector optimization based method has been proposed for classification of the heartbeat types. Electrocardiogram (ECG) signals of five different heartbeat type were used for this aim. Firstly, wavelet transform (WT) method were applied on these ECG signals to generate all feature vectors. Optimizing these feature vectors is provided by performing particle swarm optimization (PSO), genetic search, best first, greedy stepwise and multi objective evoluationary algorithms on these vectors. These optimized feature vectors are later applied to the classifier inputs for perfor

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Arumugam, Paritha, and Punam Malik. "Genetic Therapy for Beta-Thalassemia: From the Bench to the Bedside." Hematology 2010, no. 1 (2010): 445–50. http://dx.doi.org/10.1182/asheducation-2010.1.445.

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AbstractBeta-thalassemia is a genetic disorder with mutations in the β-globin gene that reduce or abolish β-globin protein production. Patients with β-thalassemia major (Cooley's anemia) become severely anemic by 6 to 18 months of age, and are transfusion dependent for life, while those with thalassemia intermedia, a less-severe form of thalassemia, are intermittently or rarely transfused. An allogeneically matched bone marrow transplant is curative, although it is restricted to those with matched donors. Gene therapy holds the promise of “fixing” one's own bone marrow cells by transferring th

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Prajwal, Aher* Sakshi Waikar Gauri Salave Pooja Gangurde Dr. Atul Bendale Dr. Anil Jadhav. "The Blueprint of Healing: Exploring the Frontiers of Gene Therapy." International Journal of Pharmaceutical Sciences 3, no. 1 (2025): 1994–98. https://doi.org/10.5281/zenodo.14725579.

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It is an approach to fixing the faulty genes that cause illness. An organism's ability to grow, develop, differentiate, and simply survive depends on the control of gene expression. This might be characterized as the transfer of genetic material to treat an illness or at the very least to enhance a patient's clinical condition. Converting viruses into genetic shuttles that will transfer the desired gene into the target cells is one of the fundamental ideas of gene therapy. Adenoviruses and adeno-associated viruses serve as the foundation for the most widely utilized DNA viral vectors. The dele

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Mastakov, Mihail Y., Kristin Baer, C. Wymond Symes, Claudia B. Leichtlein, Robert M. Kotin, and Matthew J. During. "Immunological Aspects of Recombinant Adeno-Associated Virus Delivery to the Mammalian Brain." Journal of Virology 76, no. 16 (2002): 8446–54. http://dx.doi.org/10.1128/jvi.76.16.8446-8454.2002.

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ABSTRACT Recombinant adeno-associated viruses (rAAV) are highly efficient vectors for gene delivery into the central nervous system (CNS). However, host inflammatory and immune responses may play a critical role in limiting the use of rAAV vectors for gene therapy and functional genomic studies in vivo. Here, we evaluated the effect of repeated injections of five rAAV vectors expressing different genetic sequences (coding or noncoding) in a range of combinations into the rat brain. Specifically, we wished to determine whether a specific immune or inflammatory response appeared in response to t

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Inggih, Permana, Evrilyan Rozanda Nesdi, Syafria Fadhilah, and Nur Salisah Febi. "Optimization Learning Vector Quantization Using Genetic Algorithm for Detection of Diabetics." Indonesian Journal of Electrical Engineering and Computer Science 12, no. 3 (2018): 1111–16. https://doi.org/10.11591/ijeecs.v12.i3.pp1111-1116.

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This study proposed the method to improve the result of Learning Vector Quantization (LVQ) by optimizing the weight vectors using a genetic algorithm (GA) to detect the diabetics. Initial value of individuals for GA is taken from weight vectors which come from the last m iterations of LVQ training result. The result of experiment showed that there is a significant increase in sensitivity level, however there is a significant decrease in specificity level. It means the proposed method success in improving the LVQ ability to recognized the diabetics, but it lowers the ability of LVQ to recognize

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Pless, Evlyn, Norah P. Saarman, Jeffrey R. Powell, Adalgisa Caccone, and Giuseppe Amatulli. "A machine-learning approach to map landscape connectivity inAedes aegyptiwith genetic and environmental data." Proceedings of the National Academy of Sciences 118, no. 9 (2021): e2003201118. http://dx.doi.org/10.1073/pnas.2003201118.

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Mapping landscape connectivity is important for controlling invasive species and disease vectors. Current landscape genetics methods are often constrained by the subjectivity of creating resistance surfaces and the difficulty of working with interacting and correlated environmental variables. To overcome these constraints, we combine the advantages of a machine-learning framework and an iterative optimization process to develop a method for integrating genetic and environmental (e.g., climate, land cover, human infrastructure) data. We validate and demonstrate this method for theAedes aegyptim

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Young, Won-Bin, Gary L. Lindberg, and Charles J. Link. "DNA Methylation of Helper Virus Increases Genetic Instability of Retroviral Vector Producer Cells." Journal of Virology 74, no. 7 (2000): 3177–87. http://dx.doi.org/10.1128/jvi.74.7.3177-3187.2000.

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ABSTRACT Retroviral vector producer cells (VPC) have been considered genetically stable. A clonal cell population exhibiting a uniform vector integration pattern is used for sustained vector production. Here, we observed that the vector copy number is increased and varied in a population of established LTKOSN.2 VPC. Among five subclones of LTKOSN.2 VPC, the vector copy number ranged from 1 to approximately 29 copies per cell. A vector superinfection experiment and Northern blot analysis demonstrated that suppression of helper virus gene expression decreased Env-receptor interference and allowe

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Neuwelt, Edward A., Michael A. Pagel, Alfred Geller, and Leslie L. Muldoon. "Gene replacement therapy in the central nervous system: Viral vector-mediated therapy of global neurodegenerative disease." Behavioral and Brain Sciences 18, no. 1 (1995): 1–9. http://dx.doi.org/10.1017/s0140525x00037237.

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AbstractThis target article describes the current state of global gene replacement in the brain using viral vectors and assesses possible solutions to some of the many problems inherent in gene therapy of the central nervous system (CNS). Gene replacement therapy in the CNS is a potential means of producing a stable expression of normal human proteins in deficient cells and thus curing certain genetically inherited enzyme deficiencies and metabolic diseases as well as cancers. The two major issues to be addressed in CNS gene replacement are the delivery of genetic material to the brain and the

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Altamiranda-Saavedra, Mariano, Nelson Naranjo-Díaz, Jan E. Conn, and Margarita M. Correa. "Entomological parameters and population structure at a microgeographic scale of the main Colombian malaria vectors Anopheles albimanus and Anopheles nuneztovari." PLOS ONE 18, no. 1 (2023): e0280066. http://dx.doi.org/10.1371/journal.pone.0280066.

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Population subdivision among several neotropical malaria vectors has been widely evaluated; however, few studies have analyzed population variation at a microgeographic scale, wherein local environmental variables may lead to population differentiation. The aim of the present study was to evaluate the genetic and geometric morphometric structure of Anopheles nuneztovari and Anopheles albimanus in endemic localities of northwestern Colombia. Genetic and phenetic structures were evaluated using microsatellites markers and wing geometric morphometrics, respectively. In addition, entomological ind

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Cattoglio, Claudia, Barbara Felice, Davide Cittaro, et al. "Transcription Factor Binding Sites Are Genetic Determinant of Retroviral Integration in the Human Genome." Blood 112, no. 11 (2008): 820. http://dx.doi.org/10.1182/blood.v112.11.820.820.

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Abstract Gamma-retroviruses and lentiviruses integrate non-randomly in mammalian genomes, with specific preferences for active chromatin, promoters and regulatory regions. Gamma-retroviral gene transfer vectors show a high frequency of insertional gene activation, and may induce neoplastic or pre-neoplastic clonal expansions in patients treated with genetically modified cells. An analysis of >10,000 integration sites of a Moloney leukemia virus (MLV)-derived vector in human CD34+ hematopoietic progenitors showed that genes involved the control of growth, differentiation and development of t

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Schnepp, Bruce C., K. Reed Clark, Dori L. Klemanski, Christina A. Pacak, and Philip R. Johnson. "Genetic Fate of Recombinant Adeno-Associated Virus Vector Genomes in Muscle." Journal of Virology 77, no. 6 (2003): 3495–504. http://dx.doi.org/10.1128/jvi.77.6.3495-3504.2003.

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ABSTRACT Recombinant adeno-associated virus (rAAV) vectors are promising human gene transfer vectors, because they mediate long-term gene expression in vivo. The vector DNA form responsible for sustained gene expression has not been clearly defined, but it has been presumed that the vector integrates to some degree and persists in this manner. Using two independent methods, we were unable to identify rAAV integrants in mouse muscle. In the first approach, we were unable to recover host cell-vector DNA junctions from a lambda phage library generated using transduced mouse muscle DNA that contai

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Deng, C., K. R. Thomas, and M. R. Capecchi. "Location of crossovers during gene targeting with insertion and replacement vectors." Molecular and Cellular Biology 13, no. 4 (1993): 2134–40. http://dx.doi.org/10.1128/mcb.13.4.2134-2140.1993.

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Gene targeting was used to introduce nonselectable genetic changes into chromosomal loci in mouse embryo-derived stem cells. The nonselectable markers were linked to a selectable marker in both insertion- and replacement-type vectors, and the transfer of the two elements to the Hprt locus was assayed. When insertion vectors were used as substrates, the frequency of transfer was highly dependent upon the distance between the nonselectable marker and the double-strand break in the vector. A marker located close to the vector ends was frequently lost, suggesting that a double-strand gap repair ac

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Deng, C., K. R. Thomas, and M. R. Capecchi. "Location of crossovers during gene targeting with insertion and replacement vectors." Molecular and Cellular Biology 13, no. 4 (1993): 2134–40. http://dx.doi.org/10.1128/mcb.13.4.2134.

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Gene targeting was used to introduce nonselectable genetic changes into chromosomal loci in mouse embryo-derived stem cells. The nonselectable markers were linked to a selectable marker in both insertion- and replacement-type vectors, and the transfer of the two elements to the Hprt locus was assayed. When insertion vectors were used as substrates, the frequency of transfer was highly dependent upon the distance between the nonselectable marker and the double-strand break in the vector. A marker located close to the vector ends was frequently lost, suggesting that a double-strand gap repair ac

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Xu, Youqiang, Fei Tao, Cuiqing Ma, and Ping Xu. "New Constitutive Vectors: Useful Genetic Engineering Tools for Biocatalysis." Applied and Environmental Microbiology 79, no. 8 (2013): 2836–40. http://dx.doi.org/10.1128/aem.03746-12.

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ABSTRACTConstitutive vectors are useful tools for genetic engineering. Two constitutive vectors with high levels of expression and broad host ranges were developed and used in a range ofPseudomonashosts. The vectors showed superior characteristics compared to the inducible vectors as well as the potential to be used as improved genetic tools for biocatalysis.

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Saadi, Hogir. "Gene Therapy Approaches." Qubahan Academic Journal 1, no. 1 (2021): 52–56. http://dx.doi.org/10.48161/qaj.v1n1a35.

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Gene therapy can be described broadly as the transfer of genetic material to control a disease or at least to enhance a patient's clinical status. The transformation of viruses into genetic shuttles is one of the core principles of gene therapy, which will introduce the gene of interest into the target tissue and cells. To do this, safe strategies have been invented, using many viral and non-viral vector delivery. Two major methods have emerged: modification in vivo and modification ex vivo. For gene therapeutic approaches which are focused on lifelong expression of the therapeutic gene, retro

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Amalfitano, Andrea, Michael A. Hauser, Huimin Hu, Delila Serra, Catherine R. Begy, and Jeffrey S. Chamberlain. "Production and Characterization of Improved Adenovirus Vectors with the E1, E2b, and E3 Genes Deleted." Journal of Virology 72, no. 2 (1998): 926–33. http://dx.doi.org/10.1128/jvi.72.2.926-933.1998.

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ABSTRACT Adenovirus (Ad)-based vectors have great potential for use in the gene therapy of multiple diseases, both genetic and nongenetic. While capable of transducing both dividing and quiescent cells efficiently, Ad vectors have been limited by a number of problems. Most Ad vectors are engineered such that a transgene replaces the Ad E1a, E1b, and E3 genes; subsequently the replication-defective vector can be propagated only in human 293 cells that supply the deleted E1 gene functions intrans. Unfortunately, the use of high titers of E1-deleted vectors has been repeatedly demonstrated to res

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Su, Yuanzhang, Xinfeng Guo, Hang Luo, Jingyuan Wang, and Zhen Liu. "A Boundary Scan Test Vectors Optimization Method Based on Improved GA-AO* Approach Considering Fault Probability Model." Applied Sciences 14, no. 6 (2024): 2410. http://dx.doi.org/10.3390/app14062410.

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The generation of test vectors is a key technique that affects the efficiency and fault detection rate of the boundary scan test. Aiming at the local optimal solution problem of the current common test vectors generation algorithm, this paper proposes a test vectors generation algorithm based on improved GA-AO* model, through which the test vectors are generated by using the idea of heuristic search and backtracking correction. In order to speed up the heuristic search, this paper designed a heuristic function with both prior and posterior parameters to describe the influence of typical faults

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Habel, Marie-Eve, Mathieu Drouin, and Daniel Jung. "Maintenance of Epstein–Barr virus-derived episomal vectors in the murine Sp2/0 myeloma cell line is dependent upon exogenous expression of human EBP2." Biochemistry and Cell Biology 82, no. 3 (2004): 375–80. http://dx.doi.org/10.1139/o04-037.

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Vectors carrying the origin of replication (oriP) and driving expression of the EBNA-1 protein from Epstein–Barr virus (EBV) replicate as extrachromosomal episomes in human cells. Whether these vectors can be maintained as episomes in murine cells is still controversial. Here we demonstrate that EBNA-1 expression alone was unable to maintain episomal expression of an EBV-based vector in the murine Sp2/0 cell line. However, we were able to obtain long-term episome maintenance in Sp2/0 cells after exogenously expressing human EBP2 by genetic engineering. Our results provide further evidence for

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Gong, Qi, Bin Wang, Xubiao Lu, et al. "Nicking Endonuclease-Mediated Vector Construction Strategies for Plant Gene Functional Research." Plants 9, no. 9 (2020): 1090. http://dx.doi.org/10.3390/plants9091090.

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Plant genetic engineering vectors, such as RNA interference (RNAi) and CRISPR/Cas9 vectors, are important tools for plant functional genomics. Efficient construction of these functional vectors can facilitate the study of gene function. Although some methods for vector construction have been reported, their operations are still complicated and costly. Here, we describe a simpler and low-cost vector construction method by nicking endonucleases-mediated DNA assembly (NEMDA), which uses nicking endonucleases to generate single-strand overhanging complementary ends for rapid assembly of DNA fragme

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Manisha. B. Shinde, Dr. Archana D. Kajale, Dr. Madhuri A. Channawar, and Dr. Shilpa R. Gawande. "Vector-mediated cancer gene therapy: A review." GSC Biological and Pharmaceutical Sciences 13, no. 2 (2020): 152–65. http://dx.doi.org/10.30574/gscbps.2020.13.2.0368.

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Gene therapy is the transfer of genetic material to cure a disease or at least to improve the clinical status of a patient. One of the basic concepts of gene therapy is to transform viruses into genetic shuttles, which will deliver the gene of interest into the target cells. Safe methods have been devised to do this, using several viral and non-viral vectors. Two main approaches emerged: in vivo modification and ex vivo modification. Retrovirus, adenovirus, adenoassociated virus are suitable for gene therapeutic approaches which are based on permanent expression of the therapeutic gene. Non-vi

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Manisha., B. Shinde, Archana D. Kajale Dr., Madhuri A. Channawar Dr., and Shilpa R. Gawande Dr. "Vector-mediated cancer gene therapy: A review." GSC Biological and Pharmaceutical Sciences 13, no. 2 (2020): 152–65. https://doi.org/10.5281/zenodo.4308181.

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Gene therapy is the transfer of genetic material to cure a disease or at least to improve the clinical status of a patient. One of the basic concepts of gene therapy is to transform viruses into genetic shuttles, which will deliver the gene of interest into the target cells. Safe methods have been devised to do this, using several viral and non-viral vectors. Two main approaches emerged: in vivo modification and ex vivo modification. Retrovirus, adenovirus, adenoassociated virus are suitable for gene therapeutic approaches which are based on permanent expression of the therapeutic gene. Non-vi

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Gray, Stewart M., and Nanditta Banerjee. "Mechanisms of Arthropod Transmission of Plant and Animal Viruses." Microbiology and Molecular Biology Reviews 63, no. 1 (1999): 128–48. http://dx.doi.org/10.1128/mmbr.63.1.128-148.1999.

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SUMMARY A majority of the plant-infecting viruses and many of the animal-infecting viruses are dependent upon arthropod vectors for transmission between hosts and/or as alternative hosts. The viruses have evolved specific associations with their vectors, and we are beginning to understand the underlying mechanisms that regulate the virus transmission process. A majority of plant viruses are carried on the cuticle lining of a vector’s mouthparts or foregut. This initially appeared to be simple mechanical contamination, but it is now known to be a biologically complex interaction between specifi

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