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Articles de revues sur le sujet « HSDH »

Auteur : Grafiati

Publié le 9 mars 2023

Mis à jour le 31 juillet 2025

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1

Gao, Miaomiao, Kaili Nie, Meng Qin, Haijun Xu, Fang Wang та Luo Liu. "Molecular Mechanism Study on Stereo-Selectivity of α or β Hydroxysteroid Dehydrogenases". Crystals 11, № 3 (2021): 224. http://dx.doi.org/10.3390/cryst11030224.

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Hydroxysteroid dehydrogenases (HSDHs) are from two superfamilies of short-chain dehydrogenase (SDR) and aldo–keto reductase (AKR). The HSDHs were summarized and classified according to their structural and functional differences. A typical pair of enzymes, 7α–hydroxysteroid dehydrogenase (7α–HSDH) and 7β–hydroxysteroid dehydrogenase (7β–HSDH), have been reported before. Molecular docking of 7-keto–lithocholic acid(7–KLA) to the binary of 7β–HSDH and nicotinamide adenine dinucleotide phosphate (NADP+) was realized via YASARA, and a possible binding model of 7β–HSDH and 7–KLA was obtained. The α

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Ji, Qingzhi, Jiamin Chen, Luping Zhu, Ruiyao Wang та Bochu Wang. "The Impact of Bilirubin on 7α- and 7β-Hydroxysteroid Dehydrogenases: Spectra and Docking Analysis". Catalysts 13, № 6 (2023): 965. http://dx.doi.org/10.3390/catal13060965.

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7α- and 7β-hydroxysteroid dehydrogenases (HSDHs) are enzymes that can catalyze the isomerization of hydroxyl groups at site seven of bile acids. In a previous study, we found that the activities of 7α- and 7β-HSDHs can be inhibited by bilirubin. In order to clarify the impact, the effects of bilirubin on enzymes were studied by kinetics, spectrum, and docking analysis. The relative activity of 7α-HSDH remained less than 40% under 1 mM bilirubin, and only 18% activity of 7β-HSDH kept in the same condition. Using taurochenodeoxycholic acid (TCDCA) as substrate, the Km of 7α-HSDH was up to 0.63 m

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Wang, Rui, Jiaquan Wu, David Kin Jin та ін. "Structure of NADP+-bound 7β-hydroxysteroid dehydrogenase reveals two cofactor-binding modes". Acta Crystallographica Section F Structural Biology Communications 73, № 5 (2017): 246–52. http://dx.doi.org/10.1107/s2053230x17004460.

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In mammals, bile acids/salts and their glycine and taurine conjugates are effectively recycled through enterohepatic circulation. 7β-Hydroxysteroid dehydrogenases (7β-HSDHs; EC 1.1.1.201), including that from the intestinal microbeCollinsella aerofaciens, catalyse the NADPH-dependent reversible oxidation of secondary bile-acid products to avoid potential toxicity. Here, the first structure of NADP+bound to dimeric 7β-HSDH is presented. In one active site, NADP+adopts a conventional binding mode similar to that displayed in related enzyme structures. However, in the other active site a unique b

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Ferreira, Renato Rodrigues, Ariane Vendemiatti, Lyndel Wayne Meinhardt, Peter John Lea, and Ricardo Antunes Azevedo. "Isolation of enzymes involved in threonine biosynthesis from sorghum seeds." Brazilian Journal of Plant Physiology 16, no. 2 (2004): 95–104. http://dx.doi.org/10.1590/s1677-04202004000200005.

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Cereal seeds are poor in essential amino acids, particularly lysine, tryptophan and threonine. The amino acids lysine and threonine are synthesized in the aspartate pathway. Although most of the enzymes of the aspartate pathway have been isolated and characterized in higher plant species, the metabolism of lysine and threonine is totally unknown in sorghum. We have isolated two enzymes, aspartate kinase (AK) and homoserine dehydrogenase (HSDH) from sorghum. Optimum assay conditions were established for the determination of AK and HSDH activities. The highest level of activity was observed in i

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Prabha, V., Meenakshi Gupta та K. G. Gupta. "Kinetic properties of 7α-hydroxysteroid dehydrogenase from Escherichia coli 080". Canadian Journal of Microbiology 35, № 12 (1989): 1076–80. http://dx.doi.org/10.1139/m89-180.

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Results on the kinetics of 7α-hydroxysteroid dehydrogenase 7α-HSDH showed that this enzyme could oxidize all bile acids having an –OH group at the C-7 position. Lineweaver-Burk plots showed Michaelis constant (Km) values of 0.83 and 0.12 mM for cholic acid and chenodeoxycholic acid, respectively. The effect of enzyme concentration on the reaction velocity showed a constant increase in the enzyme activity with increase in enzyme-protein concentration. 7α-HSDH was activated by Na+, K+, Ca2+, and Mn2+ ions and by reducing agents having a thiol group (dithiothreitol, 2-mercaptoethanol). Co2+, Hg2+

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Ji, Qingzhi, Bochu Wang, Chou Li, Jinglan Hao та Wenjing Feng. "Co-immobilised 7α- and 7β-HSDH as recyclable biocatalyst: high-performance production of TUDCA from waste chicken bile". RSC Advances 8, № 60 (2018): 34192–201. http://dx.doi.org/10.1039/c8ra06798h.

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Isogai, Shota, and Hiroshi Takagi. "Enhancement of lysine biosynthesis confers high-temperature stress tolerance to Escherichia coli cells." Applied Microbiology and Biotechnology 105, no. 18 (2021): 6899–908. http://dx.doi.org/10.1007/s00253-021-11519-0.

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Abstract Lysine, a nutritionally important amino acid, is involved in adaptation and tolerance to environmental stresses in various organisms. Previous studies reported that lysine accumulation occurs in response to stress and that lysine supplementation enhances stress tolerance; however, the effect of lysine biosynthesis enhancement on stress tolerance has yet to be elucidated. In this study, we confirmed that lysine supplementation to the culture medium increased intracellular lysine content and improved cell growth of Escherichia coli at high temperature (42.5 °C). Lysine-overproducing str

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Tsachaki, Maria, Arne Meyer, Benjamin Weger, et al. "Absence of 11-keto reduction of cortisone and 11-ketotestosterone in the model organism zebrafish." Journal of Endocrinology 232, no. 2 (2017): 323–35. http://dx.doi.org/10.1530/joe-16-0495.

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Zebrafish are widely used as model organism. Their suitability for endocrine studies, drug screening and toxicity assessements depends on the extent of conservation of specific genes and biochemical pathways between zebrafish and human. Glucocorticoids consist of inactive 11-keto (cortisone and 11-dehydrocorticosterone) and active 11β-hydroxyl forms (cortisol and corticosterone). In mammals, two 11β-hydroxysteroid dehydrogenases (11β-HSD1 and 11β-HSD2) interconvert active and inactive glucocorticoids, allowing tissue-specific regulation of glucocorticoid action. Furthermore, 11β-HSDs are invol

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Husen, B., N. Psonka, M. Jacob-Meisel, C. Keil, and GM Rune. "Differential expression of 17beta-hydroxysteroid dehydrogenases types 2 and 4 in human endometrial epithelial cell lines." Journal of Molecular Endocrinology 24, no. 1 (2000): 135–44. http://dx.doi.org/10.1677/jme.0.0240135.

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In the endometrium two enzymes are known to convert estradiol to its inactive metabolite estrone: microsomal 17beta-hydroxysteroid dehydrogenase type 2 (17beta-HSD2) and peroxisomal 17beta-HSD4. In order to elucidate the particular function of each of these two different enzymes, the human endometrial epithelial cell lines HEC-1-A and RL95-2 were examined with respect to the expression of 17betaHSD isozymes. They were compared with human endometrium in vivo. Non-radioactive in situ hybridization revealed both enzymes in glandular epithelial cells of human endometrium. The two cell lines were s

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Zhou, L. Y., D. S. Wang, B. Senthilkumaran та ін. "Cloning, expression and characterization of three types of 17β-hydroxysteroid dehydrogenases from the Nile tilapia, Oreochromis niloticus". Journal of Molecular Endocrinology 35, № 1 (2005): 103–16. http://dx.doi.org/10.1677/jme.1.01801.

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In order to elucidate the roles of 17β-HSDs in fish gonadal steroidogenesis, three types of 17β-HSDs (17β-HSD1, 17β-HSD8 and putative 17β-HSD12) were cloned and characterized from the Nile tilapia, Oreochromis niloticus. The cloned cDNAs of 17β-HSD type 1, 8 and 12 were 1504, 1006 and 1930 bp long, with open reading frames encoding proteins of 289, 256 and 314 aminoacids, respectively. Tissue distribution pattern analyzed by RT-PCR and Northern blot showed that 17β-HSD1 was dominantly expressed in the ovary, while the putative 17β-HSD12, one of the two duplicates found in fish, is a male speci

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Miro, P., M. L. Marin, and M. A. Miranda. "Radical-mediated dehydrogenation of bile acids by means of hydrogen atom transfer to triplet carbonyls." Organic & Biomolecular Chemistry 14, no. 9 (2016): 2679–83. http://dx.doi.org/10.1039/c5ob02561c.

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The aim of the present paper is to explore the potential of radical-mediated dehydrogenation of bile salts (BSs), which is reminiscent of the enzymatic action of hydroxysteroid dehydrogenase enzymes (HSDH).

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Miller, William G., Bruce M. Pearson, Jerry M. Wells, Craig T. Parker, Vladimir V. Kapitonov, and Robert E. Mandrell. "Diversity within the Campylobacter jejuni type I restriction–modification loci." Microbiology 151, no. 2 (2005): 337–51. http://dx.doi.org/10.1099/mic.0.27327-0.

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The type I restriction–modification (hsd) systems of 73 Campylobacter jejuni strains were characterized according to their DNA and amino acid sequences, and/or gene organization. A number of new genes were identified which are not present in the sequenced strain NCTC 11168. The closely related organism Helicobacter pylori has three type I systems; however, no evidence was found that C. jejuni strains contain multiple type I systems, although hsd loci are present in at least two different chromosomal locations. Also, unlike H. pylori, intervening ORFs are present, in some strains, between hsdR

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Huang, Xueting, Juanjuan Wang, Jing Li, et al. "Prevalence of phase variable epigenetic invertons among host-associated bacteria." Nucleic Acids Research 48, no. 20 (2020): 11468–85. http://dx.doi.org/10.1093/nar/gkaa907.

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Abstract Type I restriction-modification (R-M) systems consist of a DNA endonuclease (HsdR, HsdM and HsdS subunits) and methyltransferase (HsdM and HsdS subunits). The hsdS sequences flanked by inverted repeats (referred to as epigenetic invertons) in certain Type I R-M systems undergo invertase-catalyzed inversions. Previous studies in Streptococcus pneumoniae have shown that hsdS inversions within clonal populations produce subpopulations with profound differences in the methylome, cellular physiology and virulence. In this study, we bioinformatically identified six major clades of the tyros

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Chapman, Karen, Megan Holmes та Jonathan Seckl. "11β-Hydroxysteroid Dehydrogenases: Intracellular Gate-Keepers of Tissue Glucocorticoid Action". Physiological Reviews 93, № 3 (2013): 1139–206. http://dx.doi.org/10.1152/physrev.00020.2012.

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Glucocorticoid action on target tissues is determined by the density of “nuclear” receptors and intracellular metabolism by the two isozymes of 11β-hydroxysteroid dehydrogenase (11β-HSD) which catalyze interconversion of active cortisol and corticosterone with inert cortisone and 11-dehydrocorticosterone. 11β-HSD type 1, a predominant reductase in most intact cells, catalyzes the regeneration of active glucocorticoids, thus amplifying cellular action. 11β-HSD1 is widely expressed in liver, adipose tissue, muscle, pancreatic islets, adult brain, inflammatory cells, and gonads. 11β-HSD1 is selec

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Trivedi, Seema, та Suresh Bihari Lall. "Histoarchitecture and Δ5-3β hydroxysteroid dehydrogenase profile in ovaries of the non-pregnant, pregnant and lactating insectivorous rat-tailed bat, Rhinopoma microphyllum kinneari". Animal Biology 57, № 1 (2007): 97–114. http://dx.doi.org/10.1163/157075607780001989.

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AbstractThe histoarchitecture and profile of Δ5-3β hydroxysteroid dehydrogenase were studied in an insectivorous seasonally-breeding microchiropteran, Rhinopoma microphyllum kinneari (rattailed bat) ovaries during non-pregnant, pregnant and lactation phases. Mid-sections of follicles and ova showed variation in their diameter (0.013-0.182 mm and 0.010-0.075 mm, respectively). Though dextral and sinistral ovaries are functionally equivalent, ovulation occurs only once (alternately from one ovary) in each annual cycle. An extroverted corpus luteum (0.792 mm) was observed in either the dextral or

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Huynh Thi Yen, Ly, Suk-Youl Park, and Jeong-Sun Kim. "Cloning, crystallization and preliminary X-ray diffraction analysis of an intact DNA methyltransferase of a type I restriction–modification enzyme fromVibrio vulnificus." Acta Crystallographica Section F Structural Biology Communications 70, no. 4 (2014): 489–92. http://dx.doi.org/10.1107/s2053230x14004543.

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Independently of the restriction (HsdR) subunit, the specificity (HsdS) and methylation (HsdM) subunits interact with each other, and function as a methyltransferase in type I restriction–modification systems. A single gene that combines the HsdS and HsdM subunits inVibrio vulnificusYJ016 was expressed and purified. A crystal suitable for X-ray diffraction was obtained from 25%(w/v) polyethylene glycol monomethylether 5000, 0.1 MHEPES pH 8.0, 0.2 Mammonium sulfate at 291 K by hanging-drop vapour diffusion. Diffraction data were collected to a resolution of 2.31 Å using synchrotron radiation. T

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Zhao, Fangqing, Xiaowen Zhang, Chengwei Liang, Jinyu Wu, Qiyu Bao, and Song Qin. "Genome-wide analysis of restriction-modification system in unicellular and filamentous cyanobacteria." Physiological Genomics 24, no. 3 (2006): 181–90. http://dx.doi.org/10.1152/physiolgenomics.00255.2005.

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Cyanobacteria are an ancient group of gram-negative bacteria with strong genome size variation ranging from 1.6 to 9.1 Mb. Here, we first retrieved all the putative restriction-modification (RM) genes in the draft genome of Spirulina and then performed a range of comparative and bioinformatic analyses on RM genes from unicellular and filamentous cyanobacterial genomes. We have identified 6 gene clusters containing putative Type I RMs and 11 putative Type II RMs or the solitary methyltransferases (MTases). RT-PCR analysis reveals that 6 of 18 MTases are not expressed in Spirulina, whereas one h

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Klemme, Jobst-Heinrich, Gisela Laakmann-Ditges, and Jutta Mertschuweit. "Cellular Amino Acid Concentrations and Regulation of Aspartate Kinase in the Thermophilic Phototrophic Prokaryote Chloroflexus aurantiacus." Zeitschrift für Naturforschung C 45, no. 1-2 (1990): 74–78. http://dx.doi.org/10.1515/znc-1990-1-213.

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Aspartate kinase (AK , EC 2.7.2.4) from the thermophilic, phototrophic prokaryote, Chloroflexus aurantiacus, was partially purified and separated from homoserine dehydrogenase (HSDH, EC 1.1.1.3). The molecular weights as determined by gel filtration were 130,000 and 46,000, respectively. HSDH had a moderately high thermal stability (50% inactivation at 84 °C) and displayed its activity optimum at 72 °C. By contrast, AK had its activity optimum at 52 °C (with a break-point in the Arrhenius plot at 42 °C) and was much less thermostable (50% inactivation at 67 °C). The Km-values for aspartate and

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Mericq, V., P. Medina, E. Kakarieka, L. Márquez, M. C. Johnson та G. Iñiguez. "Differences in expression and activity of 11β-hydroxysteroid dehydrogenase type 1 and 2 in human placentas of term pregnancies according to birth weight and gender". European Journal of Endocrinology 161, № 3 (2009): 419–25. http://dx.doi.org/10.1530/eje-09-0308.

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BackgroundFetal exposure to maternal glucocorticoids may determine fetal growth and the programing of later disorders. Availability of the glucocorticoids in the placenta is regulated by the 11β-hydroxysteroid dehydrogenase (11β-HSDs) enzymes. To date, there are discrepancies with regard to cortisol (F) cord blood levels in fetuses with intrauterine growth retardation in different species.ObjectiveTo study the expression and activity of 11β-HSDs in placentas from full term small for gestational age (SGA), appropriate for gestational age (AGA) and large for gestational age (LGA) newborns, and c

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Bialevich, Vitali, Dhiraj Sinha, Katsiaryna Shamayeva, et al. "The helical domain of the EcoR124I motor subunit participates in ATPase activity and dsDNA translocation." PeerJ 5 (January 18, 2017): e2887. http://dx.doi.org/10.7717/peerj.2887.

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Type I restriction-modification enzymes are multisubunit, multifunctional molecular machines that recognize specific DNA target sequences, and their multisubunit organization underlies their multifunctionality. EcoR124I is the archetype of Type I restriction-modification family IC and is composed of three subunit types: HsdS, HsdM, and HsdR. DNA cleavage and ATP-dependent DNA translocation activities are housed in the distinct domains of the endonuclease/motor subunit HsdR. Because the multiple functions are integrated in this large subunit of 1,038 residues, a large number of interdomain cont

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Grinkevich, Pavel, Iuliia Iermak, Nicholas A. Luedtke, Jeroen R. Mesters, Rüdiger Ettrich, and Jost Ludwig. "pHluorin-assisted expression, purification, crystallization and X-ray diffraction data analysis of the C-terminal domain of the HsdR subunit of theEscherichia colitype I restriction-modification system EcoR124I." Acta Crystallographica Section F Structural Biology Communications 72, no. 9 (2016): 672–76. http://dx.doi.org/10.1107/s2053230x16011626.

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The HsdR subunit of the type I restriction-modification system EcoR124I is responsible for the translocation as well as the restriction activity of the whole complex consisting of the HsdR, HsdM and HsdS subunits, and while crystal structures are available for the wild type and several mutants, the C-terminal domain comprising approximately 150 residues was not resolved in any of these structures. Here, three fusion constructs with the GFP variant pHluorin developed to overexpress, purify and crystallize the C-terminal domain of HsdR are reported. The shortest of the three encompassed HsdR res

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Devendran, Saravanan, Sean M. Mythen, and Jason M. Ridlon. "The desA and desB genes from Clostridium scindens ATCC 35704 encode steroid-17,20-desmolase." Journal of Lipid Research 59, no. 6 (2018): 1005–14. http://dx.doi.org/10.1194/jlr.m083949.

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Clostridium scindens is a gut microbe capable of removing the side-chain of cortisol, forming 11β-hydroxyandrostenedione. A cortisol-inducible operon (desABCD) was previously identified in C. scindens ATCC 35704 by RNA-Seq. The desC gene was shown to encode a cortisol 20α-hydroxysteroid dehydrogenase (20α-HSDH). The desD encodes a protein annotated as a member of the major facilitator family, predicted to function as a cortisol transporter. The desA and desB genes are annotated as N-terminal and C-terminal transketolases, respectively. We hypothesized that the DesAB forms a complex and has st

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Shimojo, M., C. B. Whorwood та P. M. Stewart. "11β-Hydroxysteroid dehydrogenase in the rat adrenal". Journal of Molecular Endocrinology 17, № 2 (1996): 121–30. http://dx.doi.org/10.1677/jme.0.0170121.

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ABSTRACT 11β-Hydroxysteroid dehydrogenase (11β-HSD) catalyses the interconversion of biologically active cortisol to inactive cortisone in man, and corticosterone to 11-dehydrocorticosterone in rodents. As such, this enzyme has been shown to confer aldosterone-selectivity on the mineralocorticoid receptor and to modulate cortisol/corticosterone access to the glucocorticoid receptor (GR). Two kinetically distinct isoforms of this enzyme have been characterized in both rodents and man; a low-affinity NADP(H)-dependent enzyme (11β-HSD1) which predominantly acts as an oxo-reductase and, more recen

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Tetsi Nomigni, Milène, Sophie Ouzounian, Alice Benoit, et al. "Steroidogenic enzyme profile in an androgen-secreting adrenocortical oncocytoma associated with hirsustism." Endocrine Connections 4, no. 2 (2015): 117–27. http://dx.doi.org/10.1530/ec-15-0014.

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Hirsutism induced by hyperandrogenism can be associated with polycystic ovary syndrome, 21-hydroxylase (OH) deficiency or androgen-secreting tumors, including ovarian and adrenal tumors. Adrenal androgen-secreting tumors are frequently malignant. Adrenal oncocytomas represent rare causes of hyperandrogenism. The aim of the study was to investigate steroidogenic enzyme expression and steroid secretion in an androgen-secreting adrenal oncocytoma in a young woman presenting with hirsutism. Hyperandrogenism was diagnosed on the basis of elevated plasma Δ4-androstenedione and testosterone levels. P

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Legeza, Balázs, Zoltán Balázs, Lyubomir G. Nashev та Alex Odermatt. "The Microsomal Enzyme 17β-Hydroxysteroid Dehydrogenase 3 Faces the Cytoplasm and Uses NADPH Generated by Glucose-6-Phosphate Dehydrogenase". Endocrinology 154, № 1 (2013): 205–13. http://dx.doi.org/10.1210/en.2012-1778.

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Recent studies proposed a functional coupling between 17β-hydroxysteroid dehydrogenase 3 (17β-HSD3)-dependent testosterone formation and 11β-hydroxysteroid dehydrogenase 1 (11β-HSD1)-mediated interconversion of glucocorticoids through competition for the luminal pyridine nucleotide pool. To test this hypothesis, we used human embryonic kidney-293 cells transfected with 17β-HSD3 and/or 11β-HSD1, in the absence or presence of hexose-6-phosphate dehydrogenase that generates reduced nicotinamide adenine dinucleotide phosphate (NADPH) in the endoplasmic reticulum and determined enzyme activities. A

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Baumgartner, Maximilian, Michaela Lang, Marion Nehr та ін. "406: RUMINOCOCCUS GNAVUS 3β-HSDH LINKS MUCOSAL BIOFILMS AND BILE ACID MALABSORPTION". Gastroenterology 162, № 7 (2022): S—90. http://dx.doi.org/10.1016/s0016-5085(22)60227-0.

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Jang, Christina, Varuni R. Obeyesekere, Rodney J. Dilley, Zygmunt Krozowski, Warrick J. Inder та Frank P. Alford. "Altered Activity of 11β-Hydroxysteroid Dehydrogenase Types 1 and 2 in Skeletal Muscle Confers Metabolic Protection in Subjects with Type 2 Diabetes". Journal of Clinical Endocrinology & Metabolism 92, № 8 (2007): 3314–20. http://dx.doi.org/10.1210/jc.2006-2729.

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Abstract Context: There is little information regarding the regulation of 11β-hydroxysteroid dehydrogenase (11β-HSD) enzymes in skeletal muscle in the setting of type 2 diabetes. Objective: Our objective was to investigate whether there is differential mRNA expression and enzyme activity of 11β-HSD1 and 11β-HSD2 in the skeletal muscle of diabetic subjects compared with controls at baseline and in response to dexamethasone. Design: Participants underwent muscle biopsy of vastus lateralis at baseline and after dexamethasone. Setting: The study took place at a university teaching hospital. Partic

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Oliver, Melissa B., and W. Edward Swords. "Comparative Analysis of Streptococcus pneumoniae Type I Restriction-Modification Loci: Variation in hsdS Gene Target Recognition Domains." Pathogens 9, no. 9 (2020): 712. http://dx.doi.org/10.3390/pathogens9090712.

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Streptococcus pneumoniae (pneumococcus) is a respiratory commensal pathogen that causes a range of infections, particularly in young children and the elderly. Pneumococci undergo spontaneous phase variation in colony opacity phenotype, in which DNA rearrangements within the Type I restriction-modification (R-M) system specificity gene hsdS can potentially generate up to six different hsdS alleles with differential DNA methylation activity, resulting in changes in gene expression. To gain a broader perspective of this system, we performed bioinformatic analyses of Type I R-M loci from 18 publis

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Taketo-Hosotani, T., Y. Nishioka, C. M. Nagamine, I. Villalpando, and H. Merchant-Larios. "Development and fertility of ovaries in the B6.YDOM sex-reversed female mouse." Development 107, no. 1 (1989): 95–105. http://dx.doi.org/10.1242/dev.107.1.95.

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When the Y chromosome of Mus musculus domesticus (YDOM) was introduced onto the C57BL/6 (B6) mouse background, half of the XY progeny (B6.YDOM) developed bilateral ovaries and female internal and external genitalia. We examined the fertility of the B6.YDOM sex-reversed female mouse. The chromosomal sex of the individual mouse was identified by dot hybridization with mouse Y chromosome-specific DNA probes. The results indicated that all XY females lacked regular estrous cyclicity although most were able to mate and ovulate after treatment with gonadotropins. When they had been ovariectomized an

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Prabha, Vijay, Meenakshi Gupta, D. Seiffge та K. G. Gupta. "Purification of 7α-hydroxysteroid dehydrogenase from Escherichia coli strain 080". Canadian Journal of Microbiology 36, № 2 (1990): 131–35. http://dx.doi.org/10.1139/m90-023.

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Purification studies of 7α-hydroxysteroid dehydrogenase (7α-HSDH) (EC 1.1.1.159) from Escherichia coli 080 showed that 1.59-fold purification could be achieved by heating (60 °C for 10 min) the ultracentrifuged enzyme preparation, and 6.46-fold purification was achieved by subsequent precipitation with ammonium sulfate. Further purification on Sephadex G-100 gel gave 10.1-fold purification. After pooling and concentrating the active fractions obtained from the Sephadex G-100 filtration, an 11.1-fold purification was achieved using DEAE-cellulose chromatography. The purified enzyme produced a s

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Souness, Graham W., Andrew S. Brem та David J. Morris. "Antisense to Both 11β-Hsd1 and 2 Increase Glucocorticoid (GC) Activity in Vascular Tissue". Hypertension 36, suppl_1 (2000): 705. http://dx.doi.org/10.1161/hyp.36.suppl_1.705-c.

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P69 GC’s amplify the pressor effects of catecholamines and Ang II in VSM. Thus, the level of vasoconstriction in vascular tissue can be influenced by local GC concentrations. GC’s are metabolized to their inactive 11-dehydro derivatives by the enzyme 11β-HSD which exists in at least two isoforms, 11β-HSD1 and 11β-HSD2. 11β-HSD2 is unidirectional, with only dehydrogenase activity (inactivating GC’s) while 11β-HSD1 is bi-directional, also possessing reductase activity and thus the ability to reactivate 11-dehydroGC’s back to the active parent steroid. The aim of the present study was to investig

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Coutinho, Agnes E., Mohini Gray, David G. Brownstein та ін. "11β-Hydroxysteroid Dehydrogenase Type 1, But Not Type 2, Deficiency Worsens Acute Inflammation and Experimental Arthritis in Mice". Endocrinology 153, № 1 (2012): 234–40. http://dx.doi.org/10.1210/en.2011-1398.

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Glucocorticoids profoundly influence immune responses, and synthetic glucocorticoids are widely used clinically for their potent antiinflammatory effects. Endogenous glucocorticoid action is modulated by the two isozymes of 11β-hydroxysteroid dehydrogenase (11β-HSD). In vivo, 11β-HSD1 catalyzes the reduction of inactive cortisone or 11-dehydrocorticosterone into active cortisol or corticosterone, respectively, thereby increasing intracellular glucocorticoid levels. 11β-HSD2 catalyzes the reverse reaction, inactivating intracellular glucocorticoids. Both enzymes have been postulated to modulate

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Tseng, J. K., P. C. Tang, and J. C. Ju. "357 CALCIUM RELEASE INDUCED BY THIMEROSAL AND INOSITOL 1,4,5-TRIPHOSPHATE IN HEAT-SHOCKED PORCINE OOCYTES." Reproduction, Fertility and Development 19, no. 1 (2007): 294. http://dx.doi.org/10.1071/rdv19n1ab357.

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Elevated ambient temperature has been known to be deleterious to the developmental competence of mammalian oocytes and embryos, although the mechanism is still unclear. The objective of this study was to determine the effect of heat shock (HS) on the alteration of intracellular calcium concentrations ([Ca2+]i) of matured pig oocytes by two different calcium releasing agents. Porcine cumulus–oocyte complexes were aspirated from the follicles (3–6 mm) and subjected to standard in vitro maturation procedure for 42 h. Matured oocytes were then randomly allocated to different heat treatments at 41.

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Lou, Deshuai, Xiaoli Zhang, Yangyang Cao та ін. "A novel NADP(H)-dependent 3α-HSDH from the intestinal microbiome of Ursus thibetanus". International Journal of Biological Macromolecules 219 (жовтень 2022): 159–65. http://dx.doi.org/10.1016/j.ijbiomac.2022.07.252.

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Devendran, Saravanan, Celia Méndez-García та Jason M. Ridlon. "Identification and characterization of a 20β-HSDH from the anaerobic gut bacteriumButyricicoccus desmolansATCC 43058". Journal of Lipid Research 58, № 5 (2017): 916–25. http://dx.doi.org/10.1194/jlr.m074914.

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Pan, Yinping, Shijin Tang, Liancai Zhu, Deshuai Lou, Jun Tan та Bochu Wang. "Design of St-2-2 7α-HSDH mutants for altering substrate preference and thermostability". Molecular Catalysis 548 (вересень 2023): 113423. http://dx.doi.org/10.1016/j.mcat.2023.113423.

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Langlois, D. A., S. G. Matthews, M. Yu та K. Yang. "Differential expression of 11β-hydroxysteroid dehydrogenase 1 and 2 in the developing ovine fetal liver and kidney". Journal of Endocrinology 147, № 3 (1995): 405–11. http://dx.doi.org/10.1677/joe.0.1470405.

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Abstract In adult mammals, liver and kidney are the two major sites of biosynthesis for 11β-hydroxysteroid dehydrogenase (11β-HSD) 1 and 2 respectively. In the present study, the expression of these two isozymes in the developing ovine fetal liver and kidney was characterized. Livers and kidneys were obtained from fetal sheep at days 85, 100–120 and 140–143 of gestation (term=145 days). Tissue levels of 11β-HSD2 mRNA were assessed by Northern blot analysis. 11β-HSD dehydrogenase and reductase activities in tissue homogenates were determined by a radiometric conversion assay using cortisol and

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Patel, Pushpa, Rowan Hardy, Vaiyapuri Sumathi та ін. "Expression of 11β-hydroxysteroid dehydrogenase enzymes in human osteosarcoma: potential role in pathogenesis and as targets for treatments". Endocrine-Related Cancer 19, № 4 (2012): 589–98. http://dx.doi.org/10.1530/erc-12-0079.

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Osteosarcoma (OS) is a primary malignant tumour of bone occurring predominantly in children and young adults. Despite chemotherapy, relapse is common and mortality remains high. Non-transformed osteoblasts are highly sensitive to glucocorticoids, which reduce proliferation and induce apoptosis. Previously, we observed that OS cells, but not normal osteoblasts, express 11β-hydroxysteroid dehydrogenase type 2 (11β-HSD2). This enzyme inactivates cortisol (active) to cortisone (inactive) and expression of 11β-HSD2 renders OS cells resistant to glucocorticoids. By contrast, the related enzyme 11β-H

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Mark, P. J., S. Augustus, D. P. Hewitt та B. J. Waddell. "203. Partial progesterone withdrawal during late gestation increases placental expression of 11β-HSD1 in the rat". Reproduction, Fertility and Development 20, № 9 (2008): 3. http://dx.doi.org/10.1071/srb08abs203.

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Fetal glucocorticoid excess programs adverse outcomes in adult offspring, including hypertension, obesity and insulin resistance. Access of maternal glucocorticoids to the fetus is regulated by the placental glucocorticoid barrier which consists of the 11β-hydroxysteroid dehydrogenase type 2 (11β-HSD2) enzyme and P-glycoprotein (Abcb1). Both proteins act to reduce fetal and placental exposure to active, circulating glucocorticoids. In addition, placental expression of 11β-HSD1 is thought to limit the effectiveness of the barrier by local reactivation of inert glucocorticoids. The present study

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Takeda, Yoshiyu, Yoshiichi Takeda, Mitsuhiro Kometani, et al. "EPIGENESIS OF 11BETA-HYDROXYSTEROID DEHYDROGENASE IN SALT-SENSITIVE HYPERTENSIVE RATS." Journal of Hypertension 42, Suppl 1 (2024): e174. http://dx.doi.org/10.1097/01.hjh.0001021136.84476.e9.

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Objective: 11Beta-hydroxysteroid dehydrogenase type1 (11beta-HSD1) is the modulator of glucocorticoid hormone and type2 (11beta-HSD2) is the modulator of mineralocorticoid hormone. Both enzymes are related to hypertension. We investigated the effect of high salt diet on the methylation of both enzyme gene in salt-sensitive hypertensive (SSH) rats. Design and method: SSH rats were fed a high (7% NaCl) or normal (0.45%) salt chow for 4 weeks. WKY rats were used as controls. Body weight, blood pressure, plasma and urinary aldosterone concentration and PRA were measured. DNA was extracted from bot

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Lou, Deshuai, Qian Long, Cunhong Luo, et al. "A novel NAD(H)-dependent 3alpha-HSDH with enhanced activity by magnesium or manganese ions." International Journal of Biological Macromolecules 204 (April 2022): 34–40. http://dx.doi.org/10.1016/j.ijbiomac.2022.01.198.

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Li, Zhonglin, Hang Yuan, Huikuan Chu, and Ling Yang. "The Crosstalk between Gut Microbiota and Bile Acids Promotes the Development of Non-Alcoholic Fatty Liver Disease." Microorganisms 11, no. 8 (2023): 2059. http://dx.doi.org/10.3390/microorganisms11082059.

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Recently the roles of gut microbiota are highly regarded in the pathogenesis of nonalcoholic fatty liver disease (NAFLD). The intestinal bacteria regulate the metabolism of bile acids depending on bile salt hydrolase (BSH), 7-dehydroxylation, hydroxysteroid dehydrogenase (HSDH), or amide conjugation reaction, thus exerting effects on NAFLD development through bile acid receptors such as farnesoid X receptor (FXR), Takeda G-protein-coupled bile acid protein 5 (TGR5), and vitamin D receptor (VDR), which modulate nutrient metabolism and insulin sensitivity via interacting with downstream molecule

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Adamczyk-Popławska, Monika, Aneta Kondrzycka, Katarzyna Urbanek, and Andrzej Piekarowicz. "Tetra-amino-acid tandem repeats are involved in HsdS complementation in type IC restriction–modification systems." Microbiology 149, no. 11 (2003): 3311–19. http://dx.doi.org/10.1099/mic.0.26497-0.

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All known type I restriction and modification (R–M) systems of Escherichia coli and Salmonella enterica belong to one of four discrete families: type IA, IB, IC or ID. The classification of type I systems from a wide range of other genera is mainly based on complementation and molecular evidence derived from the comparison of the amino acid similarity of the corresponding subunits. This affiliation was seldom based on the strictest requirement for membership of a family, which depends on relatedness as demonstrated by complementation tests. This paper presents data indicating that the type I N

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Pan, Jiawen, Weifeng Li, Binzhao Chen, Linchuan Liu, Jianjun Zhang та Jianming Li. "Arabidopsis 3β-Hydroxysteroid Dehydrogenases/C4-Decarboxylases Are Essential for the Pollen and Embryonic Development". International Journal of Molecular Sciences 24, № 21 (2023): 15565. http://dx.doi.org/10.3390/ijms242115565.

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The biosynthesis of C27–29 sterols from their C30 precursor squalene involves C24-alkylation and the removal of three methyl groups, including two at the C4 position. The two C4 demethylation reactions require a bifunctional enzyme known as 3β-hydroxysteroid dehydrogenase/C4-decarboxylase (3βHSD/D), which removes an oxidized methyl (carboxylic) group at C4 while simultaneously catalyzing the 3β-hydroxyl→3-keto oxidation. Its loss-of-function mutations cause ergosterol-dependent growth in yeast and congenital hemidysplasia with ichthyosiform erythroderma and limb defect (CHILD) syndrome in huma

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Nomura, Kaoru, Hiroshi Demura, Nobuo Horiba, and Kazuo Shizume. "Long-term treatment of idiopathic hyperaldosteronim using trilostane." Acta Endocrinologica 113, no. 1 (1986): 104–10. http://dx.doi.org/10.1530/acta.0.1130104.

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Abstract. Three patients with idiopathic hyperaldosteronism were continuously treated with trilostane, a competitive inhibitor of adrenal 3β-hydroxysteroid dehydrogenase (3β-HSDH) (3 to 4⅔ years). Trilostane, in conjunction with antihypertensive drugs, effectively decreased plasma aldosterone levels and improved hyperaldosteronism symptoms without undesirable side effects. Trilostane continued to be effective even when treatment was continuous. Rapid ACTH testing (iv bolus of 0.25 mg α1–24 ACTH) was done on the day without trilostane after long-term treatment, and plasma levels of aldosterone

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Sadeghi, Zohre, Mahdi Fasihi-Ramandi та Saeid Bouzari. "Brucella antigens (BhuA, 7α-HSDH, FliC) in poly I:C adjuvant as potential vaccine candidates against brucellosis". Journal of Immunological Methods 500 (січень 2022): 113172. http://dx.doi.org/10.1016/j.jim.2021.113172.

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Kupczyk, Daria, Renata Studzińska, Rafał Bilski, Szymon Baumgart, Renata Kołodziejska та Alina Woźniak. "Synthesis of Novel 2-(Isopropylamino)thiazol-4(5H)-one Derivatives and Their Inhibitory Activity of 11β-HSD1 and 11β-HSD2 in Aspect of Carcinogenesis Prevention". Molecules 25, № 18 (2020): 4233. http://dx.doi.org/10.3390/molecules25184233.

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Glucocorticoid metabolism at the tissue level is regulated by two isoenzymes 11β-hydroxysteroid dehydrogenase (11β-HSD), which mutually convert biologically active cortisol and inactive cortisone. Recent research is focused on the role of 11β-HSD1 and 11β-HSD2 as autocrine factors of tumor cell proliferation and differentiation. Herein, we report the synthesis of novel 2-(isopropylamino)thiazol-4(5H)-one derivatives and their inhibitory activity for 11β-HSD1 and 11β-HSD2. The derivative containing the spiro system of thiazole and cyclohexane rings shows the highest degree of 11β-HSD1 inhibitio

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Bakonyi, Daniel, Astrid Wirtz та Werner Hummel. "Large-scale Enzymatic Synthesis of 12-Ketoursodeoxycholic Acid from Dehydrocholic Acid by Simultaneous Combination of 3α-Hydroxysteroid Dehydrogenase from Pseudomonas testosteroni and 7β-Hydroxysteroid Dehydrogenase from Collinsella aerofaciens". Zeitschrift für Naturforschung B 67, № 10 (2012): 1037–44. http://dx.doi.org/10.5560/znb.2012-0165.

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12-Keto-UDCA is an important optically active component for the drug ursodeoxycholic acid (UDCA). Starting from the three-keto compound dehydrocholic acid, the carbonyl groups at position 3 and 7 have to be reduced stereo- and regioselectively. In this case we applied two hydroxysteroid dehydrogenases for this purpose, the NAD-dependent 3α-HSDH from Pseudomonas testosteroni and the NADP-dependent 7β-hydroxysteroid dehydrogenase from Collinsella aerofaciens. Both enzymes can be produced in high yields by an Escherichia coli strain as recombinant proteins. In order to avoid impurities by the 7a-

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Sastry, S. "Variations in the epithelial cords of the ovaries of a microchiropteran bat, Hipposideros speoris (Schneider) during reproductive cycle: An enzymic approach." Journal of Cell and Animal Biology 7, no. 11 (2013): 132–37. https://doi.org/10.5281/zenodo.13431010.

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(Uploaded by Plazi for the Bat Literature Project) The ovaries of Hipposideros speoris were studied histologically and histochemically for the enzymes, 3β-hydroxysteriod dehydrogenase (3β-HSDH), Succinic dehydrogenase (SDH) and lipid from July 2005 to 2006. The interstitial cells or so called "epithelial cords" showed variations in their distribution, morphology, enzymic and their association with other ovarian structures. These cords appear to be formed in the ovarian cortex by the transformation of granulosa of the primordial follicles and small preantral follicles whose ova regress and disa

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Sastry, S. "Variations in the epithelial cords of the ovaries of a microchiropteran bat, Hipposideros speoris (Schneider) during reproductive cycle: An enzymic approach." Journal of Cell and Animal Biology 7, no. 11 (2013): 132–37. https://doi.org/10.5281/zenodo.13431010.

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(Uploaded by Plazi for the Bat Literature Project) The ovaries of Hipposideros speoris were studied histologically and histochemically for the enzymes, 3β-hydroxysteriod dehydrogenase (3β-HSDH), Succinic dehydrogenase (SDH) and lipid from July 2005 to 2006. The interstitial cells or so called "epithelial cords" showed variations in their distribution, morphology, enzymic and their association with other ovarian structures. These cords appear to be formed in the ovarian cortex by the transformation of granulosa of the primordial follicles and small preantral follicles whose ova regress and disa

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