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1

Z., Ayu Shazwani, Husnul Azan T., Wan Ezumi M.F., and Rabeta M.S. "Acute oral toxicity of squid and cuttlefish ink powder enzyme hydrolysates in Sprague-Dawley rats in accordance with the OECD Test Guideline 425." Food Research 5, no. 5 (2021): 259–64. http://dx.doi.org/10.26656/fr.2017.5(5).219.

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The objectives of this study were to determine LD50 and establish the safety of ink squid and cuttlefish hydrolysates. In the acute toxicity study, three groups of female rats were randomly assigned. One group served as the control and two groups orally received a single limiting dose (2000 mg/kg body weight) of ink hydrolysates. There were no signs of adverse toxicity observed in behavioural patterns, clinical signs, and no significant differences (p>0.05) between the control and treated rats regarding their food and water consumption and body weight for up to 14 days. The histopathologica
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Bora, Bedika, Ashim Kumar Biswas, Devendra Kumar, Faslu Rahman CK, Anand TS, and Adis Mirel Ahmed. "Assessment of Storage-Induced Quality Changes in Freeze-Dried Chicken Liver and Heart Protein Hydrolysates Produced by Lactobacilli Fermentation." Journal of Advances in Microbiology 25, no. 5 (2025): 12–20. https://doi.org/10.9734/jamb/2025/v25i5929.

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The present study evaluated the storage stability of Lactobacillus helveticus (NCDC-292) fermented, freeze-dried chicken liver and heart protein hydrolysates. The resulting chicken liver hydrolysate powder (CLHP) and chicken heart hydrolysate powder (CHHP) were stored aerobically in PET jars at room temperature (27 ± 2°C) and analyzed over a 90-day period at 30-day intervals. Parameters assessed included pH, water activity, lipid oxidation, antioxidant activity, and microbial load. No significant changes in pH were observed throughout the storage period. However, water activity and TBARS value
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Ko, Go-Eun, Na-Yeong Kwak, Ha-Eun Nam, Su-Jin Seo, and Syng-Ook Lee. "Enzymatic preparation and antioxidant activities of protein hydrolysates from defatted egg yolk." Food Science and Preservation 31, no. 3 (2024): 444–51. http://dx.doi.org/10.11002/fsp.2024.31.3.444.

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This study aimed to investigate the characteristics of protein hydrolysates derived from defatted egg yolk using various proteolytic enzymes and compare the antioxidant activity of the resulting hydrolysates. The defatted egg yolk powder was subjected to enzymatic hydrolysis using four different proteases (alcalase, bromelain, flavourzyme and neutrase), and the resulting hydrolysates were evaluated for their antioxidant properties. Through analysis of available amino group contents and sodium dodecyl sulfate-polyacrylamide gel electrophoresis, it was observed that the defatted egg yolk powder
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Embiriekah, Salem, Maja Bulatovic, Marija Gnjatovic, et al. "Comparative analysis of functionality of spray dried whey protein hydrolysates obtained by enzymatic and microbial hydrolysis." Chemical Industry 72, no. 5 (2018): 265–74. http://dx.doi.org/10.2298/hemind171121019e.

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The aim of this study was to examine the bioactive potential of hydrolysate powders produced by enzymatic and microbial hydrolysis of whey proteins followed by spray drying, in order to reveal which one of these processes result in a product with significantly improved functional properties. Hydrolysate powders produced by the two different biotechnological processes were compared based on their antioxidant (DPPH and FTC), antibacterial as well as erythrocyte membrane stabilizing activities. The performed tests revealed that the concentration of at least 178.4 mg mL-1 of the whey protein hydro
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Fawzya, Yusro Nuri, Safira M. Nursatya, Rini Susilowati, and Ekowati Chasanah. "Characteristics of Fish Protein Hydrolysate from Yellowstripe Scad (Selaroides leptolepis) Produced by a Local Microbial Protease." E3S Web of Conferences 147 (2020): 03017. http://dx.doi.org/10.1051/e3sconf/202014703017.

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Fish protein hydrolysate (FPH) containing small protein or peptides and amino acids has a great attention related to the provision of high protein foods to overcome the problem of malnutrition. This research was purposed to prepare FPH from yellowstripe scad (Selaroides leptolepis) by using a local microbial protease from Bacillus subtilis BII-1. Hydrolysis process was done in a laboratory scale (500 g minced fish) at 55oC for 6 h. The liquid hydrolysate was then spray dried using whey protein and maltodextrin at a concentration of 20 and 30% for each filler. The treatment of whey protein powd
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Mohammadi, Maryam, Hamed Hamishehkar, Marjan Ghorbani, Rahim Shahvalizadeh, Mirian Pateiro, and José M. Lorenzo. "Engineering of Liposome Structure to Enhance Physicochemical Properties of Spirulina plantensis Protein Hydrolysate: Stability during Spray-Drying." Antioxidants 10, no. 12 (2021): 1953. http://dx.doi.org/10.3390/antiox10121953.

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Encapsulating hydrolysates in liposomes can be an effective way to improve their stability and bioactivity. In this study, Spirulina hydrolysate was successfully encapsulated into nanoliposomes composed of different stabilizers (cholesterol or γ-oryzanol), and the synthesized liposomes were finally coated with chitosan biopolymer. The synthesized formulations were fully characterized and their antioxidant activity evaluated using different methods. Then, stabilization of coated nanoliposomes (chitosomes) by spray-drying within the maltodextrin matrix was investigated. A small mean diameter and
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Zarubin, Nikita Yu, Elena N. Kharenko, Olga V. Bredikhina, et al. "An Isotonic Drink Containing Pacific Cod (Gadus macrocephalus) Processing Waste Collagen Hydrolysate for Bone and Cartilage Health." Marine Drugs 22, no. 5 (2024): 202. http://dx.doi.org/10.3390/md22050202.

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Malnutrition is one of the major factors of bone and cartilage disorders. Pacific cod (Gadus macrocephalus) processing waste is a cheap and highly promising source of bioactive substances, including collagen-derived peptides and amino acids, for bone and cartilage structure stabilization. The addition of these substances to a functional drink is one of the ways to achieve their fast intestinal absorption. Collagen hydrolysate was obtained via enzymatic hydrolysis, ultrafiltration, freeze-drying, and grinding to powder. The lyophilized hydrolysate was a light gray powder with high protein conte
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Messina, Gaglio, Morghese, et al. "Microbiological Profile and Bioactive Properties of Insect Powders Used in Food and Feed Formulations." Foods 8, no. 9 (2019): 400. http://dx.doi.org/10.3390/foods8090400.

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Microbiological, nutritional and bioactive properties of edible powders obtained from Acheta domesticus (house cricket) and Tenebrio molitor (mealworm) were investigated. Except for the enterobacteria, viable bacteria were at a higher concentration in mealworm flour. The diversity evaluation carried out using MiSeq Illumina that mainly identified Citrobacter and Enterobacteriaceae in mealworm powder and members of the Porphyromonadaceae family in house cricket powder. Enterococci were identified and characterized for their safety characteristics in terms of the absence of antibiotic resistance
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Gómez-Guillén, María Carmen, Selene Pérez-García, Ailén Alemán, María Elvira López-Caballero, Carmen G. Sotelo та María Pilar Montero. "Development of a Ready-to-Eat Fish Product Enriched with Fish Oil Entrapped in a κ-Carrageenan Egg White Fish Protein Hydrolysate Dry Powder". Foods 12, № 11 (2023): 2272. http://dx.doi.org/10.3390/foods12112272.

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This work describes the development of a ready-to-eat (RTE) product based on an equal mixture of fish mince from three undervalued fish species with different fat contents and protein gelling capacity, which was enriched with fish oil entrapped in a κ-carrageenan egg white fish protein hydrolysate powder, obtained by either spray drying (SD) or heat drying (HD) at 80 °C (HD80). Previously, the spray-dried (SD) powder and heat-dried powders obtained at 45 °C, 60 °C and 80 °C (HD45, HD60 and HD80) were characterised in terms of water solubility, lipid oxidation (TBARS), hygroscopicity and ζ pote
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Мамбетова, С. Р., та А. В. Бахтиярова. "Сорбционная очистка ксилозного гидролизата активированным углем из целлолигнина". Известия СПбЛТА, № 250 (29 листопада 2024): 353–65. https://doi.org/10.21266/2079-4304.2024.250.353-365.

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Высокие требования к качеству ксилита обусловлены его применением в пищевой и фармацевтической промышленностях. Важной задачей при подготовке ксилозных растворов к гидрированию является осветление раствора, так как ксилит – это белый кристаллический порошок. Для этих целей применяются сорбционная и флокуляционная очистка получаемых растворов, а также мембранные методы. Наиболее часто используется сорбционная очистка активированным углем, обработка которым эффективна для снижения цветности получаемых растворов. В статье рассмотрена необходимость сорбционной очистки ксилозного гидролизата и пред
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11

Yoon, Hyeon-Ji, Gyu-Hyeon Park, Yu-Rim Lee, Jeong-Min Lee, Hyun-Lim Ahn, and Syng-Ook Lee. "Enzymatic preparation and antioxidant activities of protein hydrolysates from hemp (Cannabis sativa L.) seeds." Korean Journal of Food Preservation 30, no. 3 (2023): 434–45. http://dx.doi.org/10.11002/kjfp.2023.30.3.434.

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Hemp (Cannabis sativa L.) seeds have recently been attracting attention as a new high-value-added food material owing to their excellent nutritional properties, and research on the development of functional food materials using hemp seeds is actively progressing. This study aimed to evaluate the antioxidant properties of hemp seed protein hydrolysates. Protein hydrolysates were prepared from defatted hemp seed powder (HS) by enzymatic hydrolysis using five different proteases (alcalase, bromelain, flavourzyme, neutrase, and papain). 2,4,6-trinitrobenzene sulfonic acid (TNBS) assay and SDS-PAGE
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12

Hong, P. V. C., H. D. Tan, P. T. T. Thanh, M. H. Cang, D. L. Don, and L. T. Thien. "Spray drying conditions for protein hydrolysate of crocodile meat." Food Research 5, no. 1 (2020): 140–48. http://dx.doi.org/10.26656/fr.2017.5(1).367.

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The objective of this study is to screen the effects of several parameters (i.e., drying aid concentration, inlet air temperatures, and feed flow rates) on the spray drying process of crocodile meat protein hydrolysate. The results showed that the experimental parameters exhibited significant effects on recovery of dry matter, recovery of proteins and the obtained powder properties (total protein content, moisture content and antioxidant capacity). Besides, inlet air temperature and drying aid concentration also significantly impacted response variables. The conditions for maximum recovery of
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13

Kumar, Deepak, Aishwarya Mishra, Ayon Tarafdar, et al. "Protease Catalyzed Production of Spent Hen Meat Hydrolysate Powder for Health Food Applications." Journal of Food Quality 2021 (October 12, 2021): 1–9. http://dx.doi.org/10.1155/2021/9247998.

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Whole spent hen meat of Indian commercial layer bird (BV-300 breed) was enzymatically hydrolyzed using Flavourzyme® derived from Aspergillus oryzae. Different time, temperature, and pH combinations generated through response surface methodology (RSM) were tested to find the optimal hydrolysis condition at which maximum antioxidant potential and degree of hydrolysis can be achieved. Hydrolysis for 30 min at a temperature of 53.9°C and pH of 6.56 was found suitable for achieving high degree of hydrolysis and antioxidant activity. Antioxidant potential at optimized conditions was estimated at 93.
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14

Fernández-Trapiella, Angel Cubedo. "Quantitative Analysis of Methionine, Cysteine, and Lysine in Feeds by Reversephase Liquid Chromatography Using Precolumn Derivatization with 9- Fluorenylmethyl Chloroformate: Preliminary Study." Journal of AOAC INTERNATIONAL 73, no. 6 (1990): 935–39. http://dx.doi.org/10.1093/jaoac/73.6.935.

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Abstract An Improved analytical method based on precolumn derivatization with 9-fluorenylmethyl chloroformate (9-FMC) and reverse- phase liquid chromatography was developed for quantitative analysis of methionine, cysteine, and lysine In feeds. Samples of corn, whey powder, soybean meal, meat meal, and fish meal were selected for an accurate determination of these 3 amino acids. A portion of each finely ground sample was weighed and subjected to oxidation with performic acid for 16 h before hydrolysis with 6N HCI for 24 h. An aliquot of each hydrolysate was evaporated, dissolved, and diluted w
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Osiriphun, S., S. Wangtueai, P. Rachtanapun, and W. Jirarattanarangsri. "Preparation of a protein drink from fish protein hydrolysate obtained from tilapia skin waste." Food Research 6, no. 3 (2022): 21–26. http://dx.doi.org/10.26656/fr.2017.6(3).342.

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This study investigated the extraction of protein hydrolysate from tilapia skin waste and its utilisation for the preparation of a protein drink. Tilapia skin was first prepared by removing lipids using 95% ethanol. Protein hydrolysate was then extracted using 0.375% papain at 40°C for 120 mins. The physicochemical properties of the protein hydrolysate were analysed and were then used to prepare a protein drink. The analysis of shelf-life and the prediction of shelf-life of products using the accelerated shelf-life testing method (Q10) were carried out. The content of protein hydrolysate powde
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Bernier, Marie-Ève, Jacinthe Thibodeau, and Laurent Bazinet. "Enzymatic Hydrolysis of Water Lentil (Duckweed): An Emerging Source of Proteins for the Production of Antihypertensive Fractions." Foods 13, no. 2 (2024): 323. http://dx.doi.org/10.3390/foods13020323.

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Water lentil (Duckweed), an emerging protein source, is a small floating aquatic plant with agronomic and compositional characteristics rendering it a potential source of bioactive peptides. However, enzymatic hydrolysis of duckweeds has only been carried out to assess the antioxidant and antimicrobial activities of the hydrolysates. The main objectives of this study were to perform enzymatic hydrolysis of duckweed powder utilizing several enzymes and to evaluate the final antihypertensive activity of the fractions. Duckweed powder was efficiently hydrolyzed by pepsin, chymotrypsin, papain and
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A. S. Mohammed. "IMPACT OF FISH WASTE PROTEIN ENZYMETIC HYDROLYSATE ON GROWTH OF SOME OF Lactobacillus sp. AND Bifidobacterium sp." IRAQI JOURNAL OF AGRICULTURAL SCIENCES 56, Special (2025): 275–85. https://doi.org/10.36103/fe8qmt76.

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This study was aimed to investigate the impact of protein enzymatic hydrolysate extracted from waste of common carp (Cyprinus carpio) on some bacterial growth. Two type protein concentrates were prepared from defatted carp fish waste powder, the first at pH 2/4.5 and the second at pH 12/4.5. They were hydrolysed by pepsin enzyme lyophilized powder ≥ 2500 units/mg conc.1.5gm/100gm protein concentrates for (30, 60, 90, and 120) minutes. The obtained hydrolysates characterized in term of color, amino acids, minerals elements, degree of hydrolysis, solubility in addition to bacterial growth promot
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Hu, Jin, Xiao Qin Zhu, Kai Jun Wang, Jian Liang Liu, and Jia Lin Sun. "Preparation and Heat Treatment of High Purity Al(OH)3 and Sr(OH)2 Composite Powder." Key Engineering Materials 368-372 (February 2008): 1709–11. http://dx.doi.org/10.4028/www.scientific.net/kem.368-372.1709.

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The activated Al-Sr alloy powder hydrolyzed in pure water to prepare Al(OH)3 and Sr(OH)2 composite powder and had been treated at the temperature of 700°C. The structure, properties and heat treatment of composite powder were investigated by XRD, SEM, BET, TG-DTG. The experimental results showed that the hydrolysate of Al-Sr alloy powder is the composite powder of Al(OH)3 and Sr(OH)2 by means of hydrolyzation reaction. The micrograph of the composite powder is piled up by many sheet powders of 1-3μm. The specific surface area is great, up to 45.2 m2/g. The experimental results also showed that
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Birahy, Deford Cristy, Titi Candra Sunarti, and Anja Meryandini. "Selection of Proteolytic Lactic Acid Bacteria with Probiotic Properties for Fish Protein Hydrolyzate Production." Jurnal Ilmu Pertanian Indonesia 30, no. 1 (2024): 31–39. https://doi.org/10.18343/jipi.30.1.31.

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This study aimed to select a proteolytic LAB with probiotic properties that can be applied to manufacture protein hydrolysate from fish heads. Tests on 20 isolates of LAB showed that nine isolates were proteolytic and non-pathogenic. A total of 5 isolates could grow well at 0.5% bile salt stress, and 3 of them could grow at pH 3. These three isolates had antagonistic ability against Salmonella bacteria, and one isolate was sensitive to the antibiotic tested. Molecular identification of the selected LAB isolates showed a 100% sequence similarity with Pediococcus pentosaceus with accession numbe
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Bu, Ying, Wenting Xu, Wenhui Zhu, Xuepeng Li, and Laijin Su. "Effects of different of salt-reducing formulas on the quality characteristics of clam sausage." E3S Web of Conferences 213 (2020): 01016. http://dx.doi.org/10.1051/e3sconf/202021301016.

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In this paper, Meretrix meretrix Linnaeus and Nemipterus virgatus surimi were used as raw materials, and the effects of different salt-reducing formulas on the characteristics of low-sodium clam meat sausage was studied. The results showed that there was not significantly different in texture properties (except T1) and gel properties among each groups, and the groups treated with 50% KCl instead of 50% NaCl significantly decreased the sodium content. The addition of lysine, F1 (atmospheric clam enzymatic hydrolysate lyophilized powder), F2 (ultrahigh pressure clam enzymatic hydrolysate lyophil
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Zhang, Guang Xia, Qiao Yun Zhang, and Ze Min Chen. "Methyl Trichlorosilane Prepared Methyl Sodium Silicate." Applied Mechanics and Materials 184-185 (June 2012): 1064–67. http://dx.doi.org/10.4028/www.scientific.net/amm.184-185.1064.

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This paper studied how to prepare Methyl Sodium Silicate from Methyl Trichlorosilane. Methyl trichlorosilane hydrolyzed on the interface of cyclohexane and water, then hydrolysate and sodium hydroxide prepared methyl sodium silicate at molten state. Manufactrue was characterised by XRD, IR, and differential thermal analysis. The experimental results indicate that the best hydrolyze condition was the proportion of methyl trichlorosilane and water was 10~12:100 , at 5°C, lasting for 45min; the best condition of prepared methyl sodium silicate was the proportion of sodium hydroxide and hydrolysat
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Kheng, Yuen Sim, Young Liew Jeng, Maryana Mohamad Nor, and Geng Boon Jia. "The analysis of Angiotensin-I Converting Enzyme (ACE) by maitake (Grifrola frondosa) mycelia." Journal of Tropical Resources and Sustainable Science (JTRSS) 8, no. 2 (2021): 69–72. http://dx.doi.org/10.47253/jtrss.v8i2.560.

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The Maitake (Grifola frondosa) is useful in treating diseases, specifically hypertension. Research on the maitake mycelia’s biological properties, nevertheless, are limited in the literature. This study aimed to (i) produce mushroom biomass adopting submerged fermentation, and (ii) investigate the Angiotensin-I Converting Enzyme inhibitory activity. Maitake mycelia’s yield after 14 days of fermentation under controlled conditions (approx. 1.32 g/L) were freeze-dried into powder and later were hydrolysed for analyses of Angiotensin-I Converting Enzyme inhibitory activity. Current results showed
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23

Hamad, Alwani, Zahwa Kinanti, Pachara Pomsang, Mubshair Naveed, and Dwi Hartanti. "Optimizing Konjac Glucomannan Hydrolysate (KGMH) as a Natural Binder in Foam Mat Drying for Functional Herbal Powder Drink." Research in Chemical Engineering (RiCE) 4, no. 1 (2025): 18–29. https://doi.org/10.30595/rice.v4i1.266.

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Phytochemical-rich herbal beverages are typically perishable due to their liquid form. Powder conversion via foam mat drying offers improved shelf stability but requires effective natural binders. Konjac glucomannan (KGM) is a promising candidate, though its high viscosity necessitates enzymatic hydrolysis to produce functional konjac glucomannan hydrolysate (KGMH). This study investigated konjac glucomannan hydrolysate (KGMH) as a natural binder for foam mat drying of a herbal blend (Java tea, turmeric, and seed-under-leaf). The research optimized KGMH functionality by evaluating KGM source (
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Purcell, Diane, Michael A. Packer, and Maria Hayes. "Angiotensin-I-Converting Enzyme Inhibitory Activity of Protein Hydrolysates Generated from the Macroalga Laminaria digitata (Hudson) JV Lamouroux 1813." Foods 11, no. 12 (2022): 1792. http://dx.doi.org/10.3390/foods11121792.

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Seaweeds have a long history of use as both food and medicine, especially in Asian cultures. Moreover, there is growing interest in the use of seaweed ingredients and bioactive compounds in pharmaceutical and nutraceutical products. One ailment that seaweed bioactive compounds may impact is hypertension caused by the enzyme Angiotensin Converting Enzyme 1 (ACE-1; EC 3.4.15.1), found within the Renin-Angiotensin Aldosterone System (RAAS), which causes vasoconstriction of blood vessels, including veins and arteries. The aim of this paper is to generate bioactive peptide containing protein hydrol
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Khongla, Chompoonuch, Watcharaphong Thongrongklang, Pichet Sirisakwatthana, et al. "Antioxidant Activity of Cocoa Flavored Sterilized Milk Fortified with Whey Protein Hydrolysate Derived from Gastrointestinal Proteinases Digestion." Trends in Sciences 19, no. 17 (2022): 5762. http://dx.doi.org/10.48048/tis.2022.5762.

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The objectives of this work were to study the change in α-amino content and antioxidant activity of whey protein hydrolysate (WPH) using gastrointestinal proteinases (pepsin and pancreatin). In addition, whey protein hydrolysate powder (WPHP) with high antioxidant activity were produced by simulated GI digestion using an in vitro pepsin - pancreatin hydrolysis for supplementation in flavored milk. Whey protein was hydrolyzed with pepsin for 2 h followed by pancreatin for 3 h. WPH was collected to determine the α-amino content and antioxidant activity. The results showed that digested WPH conta
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Berson, R. Eric, John S. Young, Sarah N. Kamer, and Thomas R. Hanley. "Detoxification of Actual Pretreated Corn Stover Hydrolysate Using Activated Carbon Powder." Applied Biochemistry and Biotechnology 124, no. 1-3 (2005): 0923–34. http://dx.doi.org/10.1385/abab:124:1-3:0923.

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Zainol, M. K., F. W. Abdul Sukor, A. Fisal, T. C. Tuan Zainazor, M. R. Abdul Wahab, and A. I. Zamri. "Optimization of enzymatic protein hydrolysis conditions of Asiatic hard clam (Meretrix meretrix)." Food Research 5, no. 4 (2021): 153–62. http://dx.doi.org/10.26656/fr.2017.5(4).701.

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This study was aimed to optimise the Alcalase® enzymatic hydrolysis extraction of Asiatic hard clam (AHC) (Meretrix meretrix) protein hydrolysate in terms of hydrolysis time, hydrolysis temperature, hydrolysis pH, and concentration of enzyme. Protein hydrolysate produced from AHC (M. meretrix) meat was used to determine the optimum hydrolysis conditions. Hydrolysis of AHC meat was optimised using the Central Composite Design Response Surface Methodology (RSM) (CCD). The relationship between four parameters such as temperature (45 – 65°C), enzyme to substrate concentration (1 – 2%), hydrolysis
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Dąbrowska, Anna, Konrad Babij, Marek Szołtysik, and Józefa Chrzanowska. "Viability and growth promotion of starter and probiotic bacteria in yogurt supplemented with whey protein hydrolysate during refrigerated storage." Postępy Higieny i Medycyny Doświadczalnej 71 (November 22, 2017): 0. http://dx.doi.org/10.5604/01.3001.0010.5866.

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The effect of whey protein hydrolysate (WPH) addition on growth of standard yoghurt cultures and Bifidobacterium adolescentis during co-fermentation and its viability during storage at 4ºC in yoghurts has been evaluated. WPH was obtained with the use of serine protease from Y. lipolytica yeast. Stirred probiotic yoghurts were prepared by using whole milk standardized to 16% of dry matter with the addition of either whey protein concentrate, skim milk powder (SMP), WPH-SMP (ratio 1:1), WPH. The hydrolysate increased the yoghurt culture counts at the initial stage of fermentation and significant
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Teeragaroonwong, Patsupa, Chatrapa Hudthagosol, Natta Laohakunjit, Narudee Onsri, and Promluck Sanporkha. "Spray Drying of Inca Peanut Meal Protein Hydrolysate to Produce Protein Powder Drink Mix." Trends in Sciences 21, no. 11 (2024): 8371. http://dx.doi.org/10.48048/tis.2024.8371.

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The optimal conditions (maltodextrin concentration and inlet air temperature) for spray drying Inca peanut meal protein hydrolysate (IMPH) were determined. The maltodextrin concentration was varied at 5, 10 and 15 % by weight, and inlet air temperature was varied at 150, 160 and 170 °C. The IMPH was subjected to spray drying, where the feed solution was atomized into fine droplets and exposed to a hot air stream, leading to rapid drying and the formation of powder. The moisture, protein, yield, water activity and water solubility index (WSI) of IMPH powder were analyzed. The optimal condition
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Kleekayai, Thanyaporn, Aileen O’Neill, Stephanie Clarke, Niamh Holmes, Brendan O’Sullivan, and Richard J. FitzGerald. "Contribution of Hydrolysis and Drying Conditions to Whey Protein Hydrolysate Characteristics and In Vitro Antioxidative Properties." Antioxidants 11, no. 2 (2022): 399. http://dx.doi.org/10.3390/antiox11020399.

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During the generation of functional food ingredients by enzymatic hydrolysis, parameters such as choice of enzyme, reaction pH and the drying process employed may contribute to the physicochemical and bio-functional properties of the resultant protein hydrolysate ingredients. This study characterised the properties of spray- (SD) and freeze-dried (FD) whey protein hydrolysates (WPHs) generated using Alcalase® and Prolyve® under pH-stat and free-fall pH conditions. The enzyme preparation used affected the physicochemical and antioxidative properties but had no impact on powder composition, morp
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Kolo, Sefri Maria Dolfi. "Optimasi Proses Hidrolisis Rumput Laut Ulva Reticulata dengan Pelarut HNO3 untuk Produksi Bioetanol." Jurnal Riset Kimia 14, no. 1 (2023): 12–23. http://dx.doi.org/10.25077/jrk.v14i1.574.

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One alternative to make biofuels and replace petroleum-based fuels is to convert non-food ingredients from Ulva reticulata seaweed into bioethanol. Seventy percent of the earth's surface is covered by microalgae and seaweeds that can be converted into bioethanol. Ulva seaweed contains 50.3% carbohydrates in the form of heteropolysaccharides such as glucose, arabinose, rhamnose and xylose. Optimization of the seaweed hydrolysis catalyzed by HNO3 using Microwave irradiation was done by varying acid concentration (1, 3, 5, 7%), hydrolysis time (30, 40, 50, and 60 minutes), and hydrolysis temperat
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Lin, Yu-Hsin, Guan-Wen Chen, Chin Yeh, Helena Song, and Jenn-Shou Tsai. "Purification and Identification of Angiotensin I-Converting Enzyme Inhibitory Peptides and the Antihypertensive Effect of Chlorella sorokiniana Protein Hydrolysates." Nutrients 10, no. 10 (2018): 1397. http://dx.doi.org/10.3390/nu10101397.

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Hot water was used to obtain Chlorella sorokiniana hot water extract (HWE). Subsequently, this byproduct was freeze-dried, hydrolysed at 50 °C using Protease N to obtain C. sorokiniana protein hydrolysates (PN-1), and then digested with a gastrointestinal enzyme (PN-1G). The inhibitory effects of the HWE and hydrolysates against angiotensin I-converting enzyme (ACE) were investigated. The soluble protein and peptide contents were 379.9 and 179.7 mg/g, respectively, for HWE and 574.8 and 332.8 mg/g, respectively, for PN-1. The IC50 values of the HWE, PN-1, and PN-1G on ACE were 1.070, 0.035, an
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Thongrattanatrai, Kanchana, Rarisara Impaprasert, Worapot Suntornsuk, and George Srzednicki. "Effect of Ultrasonic-Assisted Enzymatic Hydrolysis on Functional Properties and Antioxidant Activity of Eri Silkworm Pupa Protein Isolate." International Journal of Food Science 2023 (December 2, 2023): 1–10. http://dx.doi.org/10.1155/2023/9409710.

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Philosamia ricini (Eri silkworm) pupa protein isolate (EPI) was utilized to prepare pupa protein hydrolysate (EPIH) through enzymatic hydrolysis. Additionally, the isolate underwent ultrasonic treatment at 20 kHz to become ultrasound pretreated EPI (EPIU), which was then enzymatically hydrolyzed to obtain ultrasound pretreated protein hydrolysate (EPIUH). The physicochemical properties of these samples were investigated, including molecular weight, solubility, foaming and emulsion properties, water- and oil-holding capacity, antioxidant activity, and color. When compared to EPI (used as the co
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Li, Penglin, Xiaoling Miao, Rongxiu Li, and Jianjiang Zhong. "In SituBiodiesel Production from Fast-Growing and High Oil ContentChlorella pyrenoidosain Rice Straw Hydrolysate." Journal of Biomedicine and Biotechnology 2011 (2011): 1–8. http://dx.doi.org/10.1155/2011/141207.

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Rice straw hydrolysate was used as lignocellulose-based carbon source forChlorella pyrenoidosacultivation and the feasibility ofin situbiodiesel production was investigated. 13.7 g/L sugar was obtained by enzymatic hydrolyzation of rice straw.Chlorella pyrenoidosashowed a rapid growth in the rice straw hydrolysate medium, the maximum biomass concentration of 2.83 g/L was obtained in only 48 hours. The lipid content of the cells reached as high as 56.3%.In situtransesterification was performed for biodiesel production. The optimized condition was 1 g algal powder, 6 mLn-hexane, and 4 mL methano
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In, Manatchaya Sungsri, Anuchita Moongngarm, Sirirat Deeseenthum, et al. "Effect of Alcalase Hydrolysis Time on Physicochemical and Functional Properties of Protein Hydrolysate Extracted from Gryllus bimaculatus Cricket Powder." Current Research in Nutrition and Food Science Journal 12, no. 3 (2024): 1466–77. https://doi.org/10.12944/crnfsj.12.3.38.

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Humans are increasingly consuming insects as an alternative source of protein, with various products being developed to improve their acceptability. Insect protein extracts are becoming notable food ingredients, with a few studies investigating the functional and biological properties of proteins from different insects. This study determined the effect of hydrolysis time on the functional, chemical, and biological activities of cricket protein hydrolysate (CPH) extracted from Gryllus bimaculatus. The cricket powder was hydrolyzed using the enzyme alcalase at 4% w/w. The hydrolysate samples wer
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Zhang, Lixia, Xiaojing Sun, Xin Lu, et al. "Characterization of Peanut Protein Hydrolysate and Structural Identification of Umami-Enhancing Peptides." Molecules 27, no. 9 (2022): 2853. http://dx.doi.org/10.3390/molecules27092853.

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Umami peptides are naturally found in various foods and have been proven to be essential components contributing to food taste. Defatted peanut powder hydrolysate produced by a multiprotease (Flavorzyme, Alcalase, and Protamex) was found to elicit an umami taste and umami-enhancing effect. The taste profiles, hydrolysis efficiency, amino acids, molecular weight distribution, Fourier transform infrared spectroscopy (FT-IR), and separation fractions obtained by ultrafiltration were evaluated. The results showed that peanut protein was extensively hydrolyzed to give mainly (up to 96.84%) free ami
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Tultabayeva, Tamara, Gulzhan Tokysheva, Gulmira Zhakupova, et al. "Enhancing Nutrition and Palatability: The Development of Cooked Sausages with Protein Hydrolysate from Secondary Raw Materials for the Elderly." Applied Sciences 13, no. 18 (2023): 10462. http://dx.doi.org/10.3390/app131810462.

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Currently, there is an increase in the use of preparations from collagen-containing raw materials in the production of meat products. The high functional and technological properties of such preparations make it possible to significantly improve the rheological properties of food products, as well as organoleptic indicators, and enrich meat products with dietary fibers. The aim of this study was to study the effect of an emulsion of 5% protein hydrolysate and 1% purslane powder on herodietic boiled sausages for the elderly. The results showed that the experimental samples of boiled sausages co
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Wongsorn, Asst Prof Dr Duanpen, and Asst Prof Dr surachai Rattanasuk. "EFFECT OF CORDYCEPS MILITARIS HYDROLYSATE ON PROBIOTIC GROWTH." Suranaree Journal of Science and Technology 31, no. 2 (2024): 030174(1–6). http://dx.doi.org/10.55766/sujst-2024-02-e02936.

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The glucogalactomannan is the main type of polysaccharide found in Cordyceps militaris that exhibits prebiotic properties. A prebiotic is a nondigestible dietary fiber that selectively stimulates probiotics’ growth and activity in the colon. Mannanase is an enzyme that hydrolyzes mannans and heteromannans to produce mannooligosaccharides which are prebiotics. This study investigated the impact of Cordyceps militaris hydrolysate, derived from mannanase hydrolysis, on the growth of probiotic bacteria. Four strains of probiotic bacteria, namely Lactobacillus plantarum TISTR 543, Lactobacillus cas
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Minj, Shayanti, and Sanjeev Anand. "Development of a spray-dried conjugated whey protein hydrolysate powder with entrapped probiotics." Journal of Dairy Science 105, no. 3 (2022): 2038–48. http://dx.doi.org/10.3168/jds.2021-20978.

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Sary, Cesar, Leandro Daniel de Paris, Daniela Miotto Bernardi, Vanessa Lewandowiski, Altevir Signor, and Wilson Rogério Boscolo. "Tilapia by-product hydrolysate powder in diets for Nile tilapia larvae." Acta Scientiarum. Animal Sciences 39, no. 1 (2017): 1. http://dx.doi.org/10.4025/actascianimsci.v39i1.32805.

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This study aimed to evaluate the digestibility of tilapia by-product protein hydrolysate powder (TBHP) from tilapia filleting by-product, and its inclusion in diets for Nile tilapia larvae. In order to determine the apparent digestibility coefficients, two diets were formulated, the reference diet and the test diet (20% of TBHP) and, to evaluate the inclusion, six diets were formulated, which were with 0.0, 2.0; 4.0; 6.0, and 8.0% of TBHP. The apparent digestibility coefficients of crude protein and gross energy were 89.5 and 98.3%, respectively. At levels above 4.0% of inclusion of TBHP, the
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Silva, V. M., L. E. Kurozawa, K. J. Park, and M. D. Hubinger. "Influence of Carrier Agents on the Physicochemical Properties of Mussel Protein Hydrolysate Powder." Drying Technology 30, no. 6 (2012): 653–63. http://dx.doi.org/10.1080/07373937.2012.657727.

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Mandecka, Anna, Anna Dąbrowska, Łukasz Bobak, and Marek Szołtysik. "Casein Hydrolysate and Casein–Iron Chelate as Natural Bioactive Compounds for Yoghurt Fortification." Applied Sciences 12, no. 24 (2022): 12903. http://dx.doi.org/10.3390/app122412903.

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The aim of the study was to apply the bioactive components casein hydrolysate and casein–iron chelate to yoghurt and to analyze their influence on product parameters. Casein was hydrolyzed with the non-commercial protease obtained from Yarrowia lipolytica yeast and subjected to chelating process with Fe2+ ions. Both preparations were introduced to yoghurt production as additives for elevation of dry matter by 2%. The control yoghurt was produced with the addition of skim milk powder. In the prepared experimental yoghurts, the content of dry matter [DM], protein, fat, as well as iron contents w
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Semenova, E. S., E. S. Simonenko, S. V. Simonenko, S. N. Zorin, and V. K. Mazo. "Hydrolysates of mare's milk proteins. Immunochemical and physico-chemical characteristics." Food systems 7, no. 3 (2024): 466–72. http://dx.doi.org/10.21323/2618-9771-2024-7-3-466-472.

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In recent years, the attention of specialists has been attracted by a significant increase in allergic diseases, especially among children. Enzymatic hydrolysis with the participation of peptidases is considered the most effective measure to reduce the allergenicity of milk proteins. The study of the features of protein proteolysis by various enzymes, aimed at establishing optimal process parameters, is the subject of research in the production of hydrolysates with specified properties: a certain peptide and amino acid profile, reduced antigenic properties. At the same time, for a correct prec
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Marinou, Dimitra, Charlotte Jacobsen, Davide Odelli, Krystalia Sarigiannidou, and Ann-Dorit Moltke Sørensen. "Production of Protein Hydrolysates from Cod Backbone Using Selected Enzymes: Evaluation of Antioxidative and Antimicrobial Activities of Hydrolysates." Marine Drugs 23, no. 3 (2025): 125. https://doi.org/10.3390/md23030125.

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In the fish industry, up to 70% of all fish end up as side-streams such as backbones, heads, and viscera. To reduce the quantities of side-streams, a higher utilization degree of fish is needed. The aim of this study was to use cod backbone for an enzymatic production of bioactive hydrolysates with antioxidative and/or antimicrobial properties. Three different enzymes were applied (Alcalase, Neutrase, and Protamex), and hydrolyses were carried out within the enzyme’s optima for pH and temperature for 0.5–6 h. The efficiency of the enzyme treatment was evaluated based on the protein extraction
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Benito-Martínez, Selma, Bárbara Pérez-Köhler, Marta Rodríguez, Jesús María Izco, José Ignacio Recalde, and Gemma Pascual. "Wound Healing Modulation through the Local Application of Powder Collagen-Derived Treatments in an Excisional Cutaneous Murine Model." Biomedicines 10, no. 5 (2022): 960. http://dx.doi.org/10.3390/biomedicines10050960.

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Wound healing includes dynamic processes grouped into three overlapping phases: inflammatory, proliferative, and maturation/remodeling. Collagen is a critical component of a healing wound and, due to its properties, is of great interest in regenerative medicine. This preclinical study was designed to compare the effects of a new collagen-based hydrolysate powder on wound repair to a commercial non-hydrolysate product, in a murine model of cutaneous healing. Circular excisional defects were created on the dorsal skin of Wistar rats (n = 36). Three study groups were established according to the
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Cha, Yong-Jun, Jin Hyeon Kim, and Daeung Yu. "Temperature Conditions for Making Savory Microencapsulation Powder from the Hydrolysate of Soy Sauce Residue." Journal of the Korean Society of Food Science and Nutrition 50, no. 6 (2021): 562–69. http://dx.doi.org/10.3746/jkfn.2021.50.6.562.

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Li, Li, Shasha Zhao, Xiaodun Liu, Zhe Xu, Dong Li, and Xiaoyu Dai. "Lyophilized powder of calf bone marrow hydrolysate liposomes improved renal anemia: In vitro and in vivo evaluation." PLOS ONE 19, no. 12 (2024): e0314811. https://doi.org/10.1371/journal.pone.0314811.

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This study aimed to find whether oral administration of calf bone marrow hydrolysate liposomes (CBMHL) can improve renal anemia. Calf bone marrow was defatted, papain hydrolyzed, liposomalized and lyophilized. Its hematopoietic ability was proved by the colony formation experiment of umbilical cord blood hematopoietic stem cells in vitro. The rat model of renal anemia was established by adenine intragastric administration, and different concentrations of CBMHL were intragastricly administrated. Blood routine and serological indexes, transcription levels of hematopoietic factors and renal patho
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Ma'mun, Sholeh, Nur Ariffa Rochmaningsih, Riqqah Nabila, and Aisyah Sumalyani. "Determination of Reducing Sugar Groups from Hydrolysis of Arthrospira platensis Microalgae using Microwaves." BERKALA SAINSTEK 12, no. 2 (2024): 57. http://dx.doi.org/10.19184/bst.v12i2.47304.

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The depletion of fossil fuel sources and increasing carbon dioxide (CO2) emissions have encouraged research into renewable energy sources. Bioethanol is an environmentally friendly energy source with considerable potential for reducing dependence on gasoline. Bioethanol is produced from the fermentation process of monosaccharides. The first and second generations of bioethanol are derived from food crops, agricultural waste, and plantation waste, whereas the third generation is produced from microalgae. Arthrospira platensis is a carbohydrate-rich microalgae. This study attempts to determine t
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Chebon, Sammy Kiplagat, Wycliffe Chisutia Wanyonyi, John Mmari Onyari, Shital Mahindra Maru, and Francis Jackim Mulaa. "Enzymatic dehairing of sheep skin: Recovery and characterization of commercially important wool hydrolysate and fats." European Journal of Sustainable Development Research 7, no. 4 (2023): em0233. http://dx.doi.org/10.29333/ejosdr/13501.

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Conventional dehairing methods in the traditional leather-making processes, consume large amounts of toxic chemicals and produce a toxic sludge/effluent, posing disposal challenges and consequently environmental pollution. The by-products of leather processing such as hair and fat, contain toxic chemicals. In this study, crude alkaline protease from <i>bacillus cereus strain 1-p, </i>was used to dehair sheepskin with up to 99.00% recovery of valuable wool and fat. The optimum temperature and pH for wool removal were found to be 30 <sup>°</sup>C and 11, respectively. The
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Chee Huei Phing and Ong Yong Chee. "EFFECTS OF ALPHA-S1-CASEIN TRYPTIC HYDROLYSATE AND L-THEANINE ON SLEEP DISORDER AND PSYCHOLOGICAL COMPONENTS: A RANDOMIZED, DOUBLE-BLIND, PLACEBO-CONTROLLED STUDY." Malaysian Journal of Public Health Medicine 19, no. 1 (2019): 47–55. http://dx.doi.org/10.37268/mjphm/vol.19/no.1/art.36.

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Sleep disorder is common among the general population. A nutritional supplement containing alpha-s1-casein tryptic hydrolysate and L-theanine was evaluated for its effects in improving sleep quality. The study was conducted using a double-blind randomized trial. Intervention group received once-daily capsules of 150 mg alpha s1 casein tryptic hydrolysate and 50 mg L-theanine, and the control group were given placebo (150 mg skimmed milk powder) for four weeks. The outcome measurements were assessed on weekly basis using PSQI, DASS-21, clinical and biochemical parameters. ANOVA test were used t
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