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Bibliographies thématiques / Image Correlation Spectroscopy / Thèses

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Thèses sur le sujet « Image Correlation Spectroscopy »

Auteur : Grafiati

Publié le 9 mars 2023

Mis à jour le 31 juillet 2025

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1

Sergeev, Mikhail. "High order autocorrelation analysis in image correlation spectroscopy." Thesis, McGill University, 2004. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=81437.

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This thesis studies optical microscopy based high order autocorrelation approaches for measuring molecular aggregation of fluorescently labeled particles in fluid systems. As the particles randomly diffuse into and out of the volume defined by the focus of a confocal laser beam illumination, the collected fluorescence intensity fluctuates. Fluorescence Correlation Spectroscopy (FCS) and Image Correlation Spectroscopy (ICS) have been used as methods which analyse temporal and spatial intensity fluctuations, and provide quantitative information of the molecular transport processes. Theore

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Nicovich, Philip R. "Widefield fluorescence correlation spectroscopy." Diss., Georgia Institute of Technology, 2010. http://hdl.handle.net/1853/33849.

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Fluorescence correlation spectroscopy has become a standard technique for modern biophysics and single molecule spectroscopy research. Here is presented a novel widefield extension of the established single-point technique. Flow in microfluidic devices was used as a model system for microscopic motion and through widefield fluorescence correlation spectroscopy flow profiles were mapped in three dimensions. The technique presented is shown to be more tolerant to low signal strength, allowing image data with signal-to-noise values as low as 1.4 to produce accurate flow maps as well as utilizi

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Kolin, David. "k-Space image correlation spectroscopy: theory, verification, and applications." Thesis, McGill University, 2008. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=21933.

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This thesis is about the use and development of new fluorescence correlation techniques to measure the dynamics, number density, and aggregation state of fluorescently labelled proteins in living cells. An extensive investigation of the accuracy and precision of temporal image correlation spectroscopy (TICS) is presented first. Using computer simulations of laser scanning microscopy image time series, the effect of spatiotemporal sampling, particle density, noise, and photobleaching of fluorophores on the recovery of transport coefficients and number densities by TICS is investigated. It is s

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4

Hébert, Benedict. "Spatio-temporal image correlation spectroscopy : development and implementation in living cells." Thesis, McGill University, 2006. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=102507.

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The object of this thesis is to develop a new extension of Image Correlation Spectroscopy (ICS) that can measure velocity vectors for flowing protein populations in living cells. This new technique, called Spatio-Temporal Image Correlation Spectroscopy (STICS), allows measurement of both diffusion coefficients and velocity vectors (magnitude and direction) from fluorescence microscopy image time series of fluorescently labeled cellular proteins via monitoring of the time evolution of the full space-time correlation function of the intensity fluctuations. By using filtering in Fourier space to

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Coppola, Stefano, Daniela Pozzi, Giulio Caracciolo, and Thomas Schmidt. "Intracellular trafficking of lipoplexes: a particle image correlation spectroscopy (PICS) study." Diffusion fundamentals 20 (2013) 27, S. 1, 2013. https://ul.qucosa.de/id/qucosa%3A13592.

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Srivastava, Mamta. "Image cross-correlation spectroscopy, development and applications on living and fixed cells." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1999. http://www.collectionscanada.ca/obj/s4/f2/dsk1/tape7/PQDD_0014/NQ40290.pdf.

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Schwartzentruber, Jeremy. "k-space image correlation spectroscopy (kICS): accuracy and precision, capabilities and limitations." Thesis, McGill University, 2011. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=97079.

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k-space image correlation spectroscopy (kICS) is a recently developed technique that can be used to measure the transport dynamics and number density of fluorescently labeled molecules in living cells, while being completely unbiased for transport measurements by fluorophore photobleaching or blinking. Whereas the precision of fluorescence correlation spectroscopy (FCS) and temporal image correlation spectroscopy (TICS) have been investigated in detail, no such study exists for kICS. In this thesis, we present a thorough characterization of the accuracy and precision of kICS for measurements o

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Guillet, Dominique. "Spatio-temporal image correlation spectroscopy: Extension to three dimensions and application to biological systems." Thesis, McGill University, 2012. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=110545.

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The object of this thesis is to present work done using spatio-temporal image correlation spectroscopy (STICS), a technique that uses fluorescence intensity fluctuations in a microscopy image time series to calculate a complete space-time correlation function in order to measure transport dynamics in cells. The time evolution of this correlation function gives information on the magnitude and direction of a flow of fluorescent particles sampled in the image series. First, a new application of STICS to plant cell biology is shown. In dividing plant cells, delivery of new cell wall material to t

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Pandžić, Elvis. "Measurement of protein transport and confinement in heterogeneous membranes by k-space image correlation spectroscopy." Thesis, McGill University, 2013. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=116842.

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This thesis presents the application of k-space Image Correlation Spec- troscopy (kICS) to the analysis of fluorescence microscopy image time series for the measurement of particle diffusion in heterogeneous membranes, composed of micro- domains. The extension, testing and application of kICS for such measurements is developed both in silico with simulation and with in vivo cellular experiments.Connections between kICS analysis and other existing fluorescent microscopy techniques used in the study of heterogeneous membranes, such as single particle tracking (SPT) and spot vary Fluorescence Cor

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Le, Andy Vinh. "Blood Microflow Characterization Using Micro-Particle Image Velocimetry and 2-Beam Fluorescence Cross-Correlation Spectroscopy." Thesis, Université d'Ottawa / University of Ottawa, 2020. http://hdl.handle.net/10393/41535.

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Blood flow through microcirculation in both simple and complex geometry has been difficult to predict due to the composition and complex behavior of blood at the microscale. Blood is a dense suspension of deformable red blood cells that is comparable in dimensions to the microchannels that it flows through. As a result, rheological properties at the microscale can vastly differ from bulk rheological properties due to non-continuum effects. To further develop our understanding of blood microflow; experimental techniques should be explored. In this work, we explore micro-particle image velocime

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Staley, Ben Paul. "Quantification of cell penetrating peptide uptake by fluorescent techniques." Thesis, University of Manchester, 2012. https://www.research.manchester.ac.uk/portal/en/theses/quantification-of-cell-penetrating-peptide-uptake-by-fluorescent-techniques(529b93cb-3550-437a-959a-4eb2eba60da3).html.

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Cell penetrating peptides have been the focus of drug delivery research for 15 years due to their apparent ability to deliver cargoes inside cells more readily than many other carriers. Using two of the most commonly studied peptides (tat47-57 and R9), the present study differs from previous work by deliberately choosing to observe uptake with lower peptide concentrations closer to potential therapeutic doses, and by implementing raster image correlation spectroscopy (RICS) on a commercial microscope to quantify uptake in parallel to other techniques such as fluorescence correlation spectrosco

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BOUZIN, MARGAUX. "Correlazione di Immagini per lo Studio di Processi Dinamici in Sistemi Biologici." Doctoral thesis, Università degli Studi di Milano-Bicocca, 2015. http://hdl.handle.net/10281/94231.

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Dynamic processes are ubiquitous in biological systems: the transport of organelles, proteins and cargoes in the micron-sized heterogeneous cellular environment mainly occurs by Brownian diffusion, while directional flow or drift phenomena contribute to enhance the diffusion-mediated intracellular trafficking and are responsible for the delivery of blood, nutrients and signaling molecules on the larger scale of whole tissues and organs. Motivated by the relevance of transport phenomena in several fields ranging from cell biology to immunology, I adopt and extend the approach of Fluorescence Im

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Sengezer, Engin Cem. "Multifunctional Nanocomposites and Particulate Composites with Nanocomposite Binders for Deformation and Damage Sensing." Diss., Virginia Tech, 2017. http://hdl.handle.net/10919/78782.

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At present, structural health monitoring efforts focus primarily on the sensors and sensing systems for detecting instances and locations of damage through techniques such as X-ray, micro CT, acoustic emission, infrared thermography, lamb wave etc., which only detect cracks at relatively large length scales and rely heavily on sensors and sensing systems which are external to the material system. As an alternative to conventional commercially available SHM techniques, the current work explores processing-structure-property relationships starting from carbon nanotube (CNT) based nanocomposites

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CAINERO, ISOTTA. "Nanoscale investigation of chromatin organization by structured illumination microscopy." Doctoral thesis, Università degli studi di Genova, 2021. http://hdl.handle.net/11567/1042958.

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This thesis work aims to present a novel approach to reconstruct Structured Illumination Microscopy, SIM, raw data and to analyze SIM reconstructed images. These new approaches will be demonstrated in the study of chromatin organization. The dissertation will be articulated as follows: Chapter 1 provides an introduction to chromatin nanoscale organization and optical fluorescence microscopy, which is one of the main tools involved in life sciences studies. Indeed, optical microscopy allowed investigating, with high specificity and sensitivity, living samples such as cells, and even tissues.

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Desrier, Antoine. "Dynamique ultrarapide corrélée : théorie, simulations et interprétations d'expériences de spectroscopie "attoseconde"." Thesis, Sorbonne université, 2018. http://www.theses.fr/2018SORUS494.

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L'objet de cette thèse est l'étude, à des échelles de temps de l'ordre ou inférieure à la femtoseconde, des corrélations dans la dynamique de photoionisation d'atomes et de molécules. Dans un premier temps, nous mettons en évidence l’influence des corrélations vibroniques donnant une asymétrie dans la dynamique d’ionisation de molécules diatomiques. Au travers de simulations numériques impliquant la résolution de l’équation de Schrödinger dépendant du temps, nous pouvons identifier l'influence de l’échange d’énergie se produisant entre le photoélectron et les noyaux sur cette dynamique. Plus e

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Lopes, Tátila Martins 1985. "Avaliação do perfil neuropsicológico de pacientes com ataxia espinocerebelar Tipo 3 = correlações com neuroimagem estrutural, espectroscopia, e expansão de CAG = Neuropsychological profile assessment of patients with spinocerebelar ataxia type 3 : correlations with structural and spectroscopy neuroimaging and CAG lenghts." [s.n.], 2012. http://repositorio.unicamp.br/jspui/handle/REPOSIP/309310.

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Orientadores: Fernando Cendes, Marcio Luiz Figueredo Balthazar<br>Dissertação (mestrado) - Universidade Estadual de Campinas, Faculdade de Cências Médicas<br>Made available in DSpace on 2018-08-20T20:16:05Z (GMT). No. of bitstreams: 1 Lopes_TatilaMartins_M.pdf: 971844 bytes, checksum: c5993413c16c861553c1a2994f3aba69 (MD5) Previous issue date: 2012<br>Resumo: A doença de Machado Joseph ou ataxia espinocerebelar tipo 3 (DMJ/SCA3) se caracteriza pela marcha atáxica e diversos sinais extracerebelares. Manifestações neuropsiquiátricas e cognitivas já foram descritas, porém não há um consenso qua

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Sreenivasappa, Harini Bytaraya. "Raster Image Correlation Spectroscopy [RICS] Analysis of HeLa cells." 2009. http://hdl.handle.net/1969.1/ETD-TAMU-2009-12-7590.

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The objective of the project is to use the RICS analysis technique in complement with confocal microscopy to determine the diffusion coefficient of the selectively labeled green fluorescent protein (GFP), GFP-EGFR and GFP-p53 in cervical cancer cells. This is a collaboration work with MD Anderson Cancer Center. The application of the study is to lay the foundation for further study in understanding the cell metabolism, subcellular morphologic and dynamic biochemical processes to aid in the diagnosis and to potentially screen cancers. Fluorescence microscopy techniques have been developed for t

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Hong, Sung Min. "Microfluidics for Single Molecule Detection and Material Processing." Thesis, 2012. http://hdl.handle.net/1969.1/ETD-TAMU-2012-08-11726.

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In the cancer research, it is important to understand protein dynamics which are involved in cell signaling. Therefore, particular protein detection and analysis of target protein behavior are indispensable for current basic cancer research. However, it usually performed by conventional biochemical approaches, which require long process time and a large amount of samples. We have been developed the new applications based on microfluidics and Raster image Correlation spectroscopy (RICS) techniques. A simple microfluidic 3D hydrodynamic flow focusing device has been developed for quantitative

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