Littérature scientifique sur le sujet « Instrumentation for EIS »

Abstract. Electrical impedance spectroscopy (EIS), in which a sinusoidal test voltage or current is applied to the sample under test to measure its impedance over a suitable frequency range, is a powerful technique to investigate the electrical properties of a large variety of materials. In practice, the measured impedance spectra, usually fitted with an equivalent electrical model, represent an electrical fingerprint of the sample providing an insight into its properties and behavior. EIS is used in a broad range of applications as a quick and easily automated technique to characterize solid, liquid, semiliquid, organic as well as inorganic materials. This paper presents an updated review of EIS main implementations and applications.

Point-of-Care (POC) testing for biomarker detection demands techniques that are easy to use, readily available, low-cost, and with rapid response times. This paper describes the development of a fully open-source, modular, wireless, battery-powered, smartphone-controlled, low-cost potentiostat capable of conducting electrochemical impedance spectroscopy for the electrochemical detection of the S100B protein captured in an ANTI-S100B functionalized thin-film gold interdigitated electrode platform to support traumatic brain injury diagnosis and treatment. EIS results from the developed potentiostat were validated with a commercial benchtop potentiostat by comparing impedance magnitude and phase values along the EIS frequency range. In addition, an experimental design was performed for detecting S100B in spiked human plasma samples with S100B concentrations of clinical utility, and a calibration curve was found for quantifying S100B detection. No statistically significant differences were found between EIS results from the developed potentiostat and the commercial potentiostat. Statistically significant differences in the changes in charge transfer resistance signal between each tested S100B concentration (p < 0.05) were found, with a limit of detection of 35.73 pg/mL. The modularity of the proposed potentiostat allows easier component changes according to the application demands in power, frequency excitation ranges, wireless communication protocol, signal amplification and transduction, precision, and sampling frequency of ADC, among others, when compared to state-of-the-art open-source EIS potentiostats. In addition, the use of minimal, easy acquirable open-source hardware and software, high-level filtering, accurate ADC, Fast Fourier Transform with low spectral leakage, wireless communication, and the simple user interface provides a framework for facilitating EIS analysis and developing new affordable instrumentation for POC biosensors integrated systems.

The recent advent of free-electron laser (FEL) sources is driving the scientific community to extend table-top laser research to shorter wavelengths adding elemental selectivity and chemical state specificity. Both a compact setup (mini-TIMER) and a separate instrument (EIS-TIMER) dedicated to four-wave-mixing (FWM) experiments has been designed and constructed, to be operated as a branch of the Elastic and Inelastic Scattering beamline: EIS. The FWM experiments that are planned at EIS-TIMER are based on the transient grating approach, where two crossed FEL pulses create a controlled modulation of the sample excitations while a third time-delayed pulse is used to monitor the dynamics of the excited state. This manuscript describes such experimental facilities, showing the preliminary results of the commissioning of the EIS-TIMER beamline, and discusses original experimental strategies being developed to study the dynamics of matter at the fs–nm time–length scales. In the near future such experimental tools will allow more sophisticated FEL-based FWM applications, that also include the use of multiple and multi-color FEL pulses.

Zusammenfassung Während die prinzipielle Eignung der elektrischen Impedanzspektroskopie (EIS) zur Charakterisierung und Zustandsüberwachung von Materialsystemen gesichert ist, bestehen bei der Reproduzierbarkeit und der Unsicherheit von EIS-Messungen in Feldanwendungen vielfach noch Erkenntnislücken. Dies gilt besonders bei Schüttgütern - wir interessieren uns speziell für Gießereisande -, weil man Schwankungen bei der Schüttungsdichte oder der Befüllungshöhe von Messzellen erwarten darf. Wir haben diese offene Frage der Messunsicherheit mit einer Messzelle untersucht, bei der zwischen zwei planparallelen Elektroden wiederholt Schüttungen eingefüllt und die resultierende Impedanz der Anordnung in einem Frequenzbereich von 20 Hz bis 1MHz gemessen wurde. Die Unsicherheit der EIS-Gerätschaften an sich wurde über Wiederholungsmessungen bei gleichbleibender Messzellenfüllung bestimmt. Der Einfluss des Befüllungsvorgangs und damit der Schüttungszusammensetzung auf die Gesamtunsicherheit wurde über mehrmalige Befüllungs- Entleerungs-Zyklen mit ein und demselben Schüttgut untersucht. Es konnte gezeigt werden, dass die Gesamtmessunsicherheit klein genug ist, dass eine Klassifizierung verschiedener Sandzustände, sei es mit klassischen Methoden oder mit Hilfe von KI-Methoden, machbar erscheint.

Abstract. For the self-test of semiconductor gas sensors, we combine two multi-signal processes: temperature-cycled operation (TCO) and electrical impedance spectroscopy (EIS). This combination allows one to discriminate between irreversible changes of the sensor, i.e., changes caused by poisoning, as well as changes in the gas atmosphere. To integrate EIS and TCO, impedance spectra should be acquired in a very short time period, in which the sensor can be considered time invariant, i.e., milliseconds or less. For this purpose we developed a Fourier-based high-speed, low-cost impedance spectroscope. It provides a binary excitation signal through an FPGA (field programable gate array), which also acquires the data. To determine impedance spectra, it uses the ETFE (empirical transfer function estimate) method, which calculates the impedance by evaluating the Fourier transformations of current and voltage. With this approach an impedance spectrum over the range from 61 kHz to 100 MHz is acquired in ca. 16 μs. We carried out TCO–EIS measurements with this spectroscope and a commercial impedance analyzer (Agilent 4294A), with a temperature cycle consisting of six equidistant temperature steps between 200 and 450 °C, with lengths of 30 s (200 °C) and 18 s (all others). Discrimination of carbon monoxide (CO) and methane (CH4) is possible by LDA (linear discriminant analysis) using either TCO or EIS data, thus enabling a validation of results by comparison of both methods.

The evolution of impedance measurement methods into the current state of the art is reviewed briefly, and recent efforts to develop new instruments to make electrochemical impedance spectroscopy (EIS) measurements faster and more accurate are described. The most recent approach for impedance measurement uses a multichannel detection technique, which is analogous to a spectroscopic measurement such as in Fourier transform infrared spectroscopy. This method, which is capable of making impedance measurements in real time during an electrochemical experiment, allows us to come up with a new integrated equation that makes a full description of an electrochemical system possible.

Electrochemical impedance spectroscopy (EIS) is a powerful technique used for the analysis of interfacial properties related to bio-recognition events occurring at the electrode surface, such as antibody–antigen recognition, substrate–enzyme interaction, or whole cell capturing. Thus, EIS could be exploited in several important biomedical diagnosis and environmental applications. However, the EIS is one of the most complex electrochemical methods, therefore, this review introduced the basic concepts and the theoretical background of the impedimetric technique along with the state of the art of the impedimetric biosensors and the impact of nanomaterials on the EIS performance. The use of nanomaterials such as nanoparticles, nanotubes, nanowires, and nanocomposites provided catalytic activity, enhanced sensing elements immobilization, promoted faster electron transfer, and increased reliability and accuracy of the reported EIS sensors. Thus, the EIS was used for the effective quantitative and qualitative detections of pathogens, DNA, cancer-associated biomarkers, etc. Through this review article, intensive literature review is provided to highlight the impact of nanomaterials on enhancing the analytical features of impedimetric biosensors.

This study presents a system for assessing the freshness of meat with electrical impedance spectroscopy (EIS) in the frequency range of 125 Hz to 128 kHz combined with an image classifier for non-destructive and low-cost applications. The freshness standard is established by measuring the aerobic plate count (APC), 2-thiobarbituric acid reactive substances (TBARS), and composition analysis (crude fat, crude protein, and moisture) values of the microbiological detection to represent the correlation between EIS and meat freshness. The EIS and images of meat are combined to predict the freshness with the Adaboost classification and gradient boosting regression algorithms. As a result, when the elapsed time of beef storage for 48 h is classified into three classes, the time prediction accuracy is up to 85% compared to prediction accuracy of 56.7% when only images are used without EIS information. Significantly, the relative standard deviation (RSD) of APC and TBARS value predictions with EIS and images datum achieves 0.890 and 0.678, respectively.

A ruthenium oxide (RuOx) electrode was used to monitor contractile events of human pluripotent stem cells-derived cardiomyocytes (hPSC-CMs) through electrical impedance spectroscopy (EIS). Using RuOx electrodes presents an advantage over standard thin film Pt electrodes because the RuOx electrodes can also be used as electrochemical sensor for pH, O2, and nitric oxide, providing multisensory functionality with the same electrode. First, the EIS signal was validated in an optically transparent well-plate setup using Pt wire electrodes. This way, visual data could be recorded simultaneously. Frequency analyses of both EIS and the visual data revealed almost identical frequency components. This suggests both the EIS and visual data captured the similar events of the beating of (an area of) hPSC-CMs. Similar EIS measurement was then performed using the RuOx electrode, which yielded comparable signal and periodicity. This mode of operation adds to the versatility of the RuOx electrode’s use in in vitro studies.

A health condition called “Oxidative Stress” (OS), resulting from an excessive level of Reactive Oxygen Species (ROS) is a “state harmful to the body, which arises when oxidative reactions exceed antioxidant reactions because the balance between them has been lost”[1] OS appears to be associated with and might be a cause of, many serious diseases such as cardio-vascular accidents, cancer, Parkinson’s and Alzheimer’s[2]. This is not surprising as ROS are free oxygen radicals that can attack lipids, proteins, cellular membranes, enzymes and even modify DNA. Extensive correlation studies have shown that the complex impedance spectrum of blood samples from patients diagnosed with an OS syndrome differs significantly from the spectra obtained from the blood of healthy people, which is quite normal as the presence of an excessive amount of ROS should affect the physico-chemical properties of a blood sample. Measuring the complex impedance spectrum of a blood sample can be done quickly by means of low-cost electronic devices, making possible and affordable the early detection of OS among a large population. In order to quantitatively evaluate the OS, the impedance spectra being insufficient, the concentration of oxidative stress markers such as hydrogen peroxyde, malondialdehyde or F2 isoprostanes needs to be measured. Such measurements can, for instance, be used for monitoring the severity of a disease during a treatment. These concentration measurements are traditionally based upon analytical techniques but recently biosensors acting as transducers transforming directly a specific biochemical reaction into a measurable signal have been developed. They are essentially obtained by modifying the surface of metal or carbon electrodes using biomaterials such as enzymes antibodies or DNA that allow bindings or catalytic reactions with other specific biomaterials to occur on the surface of the electrodes. The resulting modifications of the electrical properties of the medium separating the electrodes can be analyzed through ad-hoc electronic and signal processing systems to yield the desired concentration. Biosensors have the advantages of rapid analysis, low-ost and high-precision. They are widely used in various fields, such as medical care, disease diagnosis and food analysis [3]. Hydrogen peroxide (H2O2) generated by cellular processes directly via two-electron reduction of molecular oxygen or indirectly via dismutation of superoxide, is the most widely studied ROS and its overproduction results in OS. Therefore, an ability to quantify the level of hydrogen peroxide and by ricochet the assessment of oxidative stress can be useful in order to assess certain health conditions occurring inside the body and as a result, an integrated electrochemical biosensor coupled with the hydrogen peroxide quantification can become a practical solution as a point of care device at home[4] Most of the time, H2O2 biosensors are based on HRP (Horseradish peroxidase) which is the most commonly used enzyme in the design of biosensors that can supervise the activity of oxidases and determine in terms of concentration, oxidase substrate such as lactate oxidase, cholesterol oxidase, or glucose oxidase, which all induce the production of hydrogen peroxide (HRP’s substrate). In the first part of this research, we explore the development of low-cost and compact measurement systems aiming to determining the impedance of biological samples as they grant access to information from electrical cellular characteristics. It is indeed possible to measure capacitance or conductance that are dependent on the health state of cells. The development of such measurement systems allowing the portability of biological essays requires sensitive electronics. Afterward, in the second part of our work, we explore the design of an electrochemical biosensor by immobilizing an enzyme (HRP) onto the surface of golden electrodes in order to detect and assess the analyte, hydrogen peroxide (H2O2). We also discuss the design of a potentiostat readout circuit to measure and convert the biosensor’s current. The combined results of the two parts of this work can be considered as a first prototype of a low cost and robust instrument easy to use in the field, away from a biological laboratory, with the goal of reaching the so called “point of care diagnostic” [5] The present thesis is organized as follows: Chapter I, introduces the present thesis. In Chapter II, we provide an overview in the field of biosensing technology. Chapter III deals with the design of a portable EIS measurement system to investigate reactive oxygen species in blood. Chapter IV presents an improved version of the previously designed instrument. Moreover, it points out the significance of EIS-based blood analysis through relevant medical diagnosis parameters such as hematocrit and erythrocyte sedimentation rate, extracted from the measured impedance spectra. In Chapter V we discuss on one hand the design of the H2O2 biosensor, and on the other hand the realization of the front-end circuit of the amperometric sensor. Finally, in Chapter VI, a conclusion is drawn..

Les rayons cosmiques sont des particules chargées, ainsi que des coproduits comme les photons et les neutrinos, issus de sources de rayons cosmiques galactiques ou extragalactiques. Ils arrivent au sommet de l'atmosphère terrestre avec des énergies primaires allant jusqu'à quelques 10 EeV. Lorsque les rayons cosmiques entrent dans l'atmosphère, ils interagissent avec les molécules de l'air et produisent un grand nombre de particules secondaires, créant une gerbe atmosphérique (extensive air shower, EAS). Accompagné des particules secondaires, une émission de la lumière Cherenkov et de la lumière fluorescence est induite par le passage des particules dans l'atmosphère. L'Observatoire Pierre Auger et Large High Altitude Air Shower Observatory (LHAASO) sont des observatoires dédiés à la détection des gerbes atmosphériques dans le but de répondre aux questions ouvertes concernant les rayons cosmiques, mais se concentrant sur différentes gammes d'énergie, les plus hautes énergies et les énergies autour de quelques PeV. Après plus de 10 ans d'exploitation de l'Observatoire Pierre Auger, la collaboration Auger a proposé une amélioration des détecteurs de son réseau de surface, appelée "AugerPrime". Le but est d'augmenter la sensibilité à la masse des particules primaires en ajoutant un détecteur scintillateur sur le détecteur Cherenkov à eau. Les deux observatoires sont dits «hybrides» car composés de télescopes optiques observant le développement longitudinal des gerbes et des réseaux de détecteurs de surface échantillonnant leurs profils latéraux. Dans cette thèse, une série d'études contribuant aux projets AugerPrime et LHAASO sont présentées. En ce qui concerne le projet AugerPrime, la présente étude comprend le travail de recherche & développement des scintillateurs et l'analyse de données du réseau de tester. Pour le projet LHAASO, des simulations de télescopes Cherenkov et une analyse multivariée des observations hybrides pour l'identification des masses primaires sont présentées
Cosmic rays are charged particles, as well as coproducts like photons and neutrinos, originated in cosmic-ray sources inside or outside the Galaxy. They arrive at the top of the Earth's atmosphere with primary energies of up to a few 10 EeV. When the cosmic rays enter the atmosphere, they interact with the molecules in the air and produce a large number of secondary particles, creating an extensive air shower (EAS). The ground-based observation of the EAS can be used to deduce the energy, the arrival direction, and the mass composition of cosmic rays. The Pierre Auger Observatory and the Large High Altitude Air Shower Observatory (LHAASO) are both EAS observatories aiming at solving open questions of cosmic-ray studies but focusing on different energy ranges, the highest-energy and the so-called knee (around few PeV) regions. Based on the experience gained during the operation of the Pierre Auger Observatory for more than 10 years, the Auger collaboration has proposed an upgrade project, called ''AugerPrime'', with the aim of increasing the sensitivity of the surface detector array to the primary mass of cosmic rays. Both observatories employ the so-called ''hybrid detector arrays'' composed of optical telescopes overlooking the longitudinal development and ground detector arrays sampling the signal densities in the lateral direction of the EAS. The ground detector arrays of both observatories are being constructed or upgraded to have various types of particle detectors (scintillator and water-Cherenkov detectors), which allow us to decompose the electromagnetic and muonic components of the EAS. In this thesis, a series of studies contributing to the AugerPrime and LHAASO projects are presented. Concerning the AugerPrime project, the present study includes R&D work of the scintillator detector and data analysis of the engineering array. For the LHAASO project, simulations of the wide field of view Cherenkov telescope array and a multivariate analysis of LHAASO-hybrid observations for the primary mass identification are presented

Recognizing the challenges small groups have to program a major work, in 2010, Joachim Linckelmann created a chamber ensemble arrangement of Johannes Brahms's "Ein deutsches Requiem." In 1869, J.M. Reiter-Biedermann published Brahms's four-hand piano arrangement of "Ein deutsches Requiem." Brahms's arrangement serves as an excellent comparison to the chamber ensemble version by Linckelmann, since it can be assumed that Brahms chose to highlight and focus on the parts he deemed the most important. This study was a comparative analysis of the two arrangements and was completed in three stages. The first stage documented every significant change in Joachim Linckelmann's recent chamber arrangement. The second stage classified each change as either a reduction, reorganization, or elimination. The final stage of the analysis was to compare the choices made by Linckelmann to those made by Brahms. The results show that Linckelmann's choices for reduction, reorganization, and elimination closely align with those of Brahms. The only differences between the arrangements can be attributed to Linckelmann's focus on retaining the original orchestral timbre and Brahms's focus on providing the original vocal parts.

Electrical Impedance Tomography (EIT) is a non linear inverse problem in which the electrical conductivity or resistivity distribution across a closed domain of interest is reconstructed from the surface potentials measured at the domain boundary by injecting a constant sinusoidal current through an array of surface electrodes. Being a non-invasive, non-radiating, non-ionizing, portable and inexpensive methodology, EIT has been extensively studied in medical diagnosis, biomedical engineering, biotechnology, chemical engineering, industrial and process engineering, civil and material engineering, soil and rock science, electronic industry, defense field, nano-technology and many other fields of applied physics. The reconstructed image quality in EIT depends mainly on the boundary data quality and the performance of the reconstruction algorithm used. The boundary data accuracy depends on the design of the practical phantoms, current injection method and boundary data measurement process and precision. On the other hand, the reconstruction algorithm performance is highly influenced by the mathematical modeling of the system, performance of the forward solver and Jacobian computation, inverse solver and the regularization techniques. Hence, for improving the EIT system performance, it is essential to improve the design of practical phantom, instrumentation and image reconstruction algorithm. As the electrical impedance of biological materials is a function of tissue composition and the frequency of applied ac signal, the better assessment of impedance distribution of biological tissues needs multifrequency EIT imaging. In medical EIT, to obtain a better image quality for a complex organ or a body part, accurate domain modelling with a large 3D finite element mesh is preferred and hence, the computation speed becomes very expensive and time consuming. But, the high speed reconstruction with improved image quality at low cost is always preferred in medical EIT. In this direction, a complete multifrequency multifunction EIT (MfMf-EIT) system is developed and multifrequency impedance reconstruction is studied to improve the bioimpedance imaging. The MfMf-EIT system consists of an MfMf-EIT instrumentation (MfMf-EITI), high speed impedance image reconstruction algorithms (IIRA), a Personal Computer (PC) and a number of practical phantoms with EIT sensors or electrodes. MfMf-EIT system and high speed IIRA are studied tested and evaluated with the practical phantoms and the multifrequency impedance imaging is improved with better image quality as well as fast image reconstruction. The MfMf-EIT system is also applied to the human subjects and the impedance imaging is studied for human body imaging and the system is evaluated. MfMf-EIT instrumentation (MfMf-EITI) consists of a multifrequency multifunction constant current injector (MfMf-CCI), multifrequency multifunction data acquisition system (MfMf DAS), a programmable electrode switching module (P-ESM) and a modified signal conditioner blocks (M-SCB) or data processing unit (DPU). MfMf-CCI, MfMf-DAS, P-ESM and M-SCBs are interfaced with a LabVIEW based data acquisition program (LV-DAP) controlled by a LabVIEW based graphical user interface (LV-GUI). LV-GUI controls the current injection and data acquisition with a user friendly, fast, reliable, efficient measurement process. The data acquisition system performance is improved by the high resolution NIDAQ card providing high precision measurement and high signal to noise ratio (SNR). MfMf-EIT system is developed as a versatile data acquisition system with a lot of flexibilities in EIT parameter selection that allows studying the image reconstruction more effectively. MfMf-EIT instrumentation controls the multifrequency and multifunctioned EIT experimentation with a number of system variables such as signal frequency, current amplitude, current signal wave forms and current injection patterns. It also works with either grounded load CCI or floating load CCI and collects the boundary data either in grounded potential form or differential form. The MfMf-EITI is futher modified to a battery based MfMf-EIT (BbMfMf-EIT) system to obtain a better patient safety and also to improve the SNR of the boundary data. MfMf-EIT system is having a facility of injecting voltage signal to the objects under test for conducting the applied potential tomography (APT). All the electronic circuit blocks in MfMf-EIT instrumentation are tested, evaluated and calibrated. The frequency response, load response, Fast Fourier Transform (FFT) studies and DSO analysis are conducted for studying the electronic performance and the signal quality of all the circuit blocks. They are all evaluated with both the transformer based power supply (TBPS) and battery based power supply (BBPS). MfMf-DAS, P-ESM and LV-DAP are tested and evaluated with digital data testing module (DDTM) and practical phantoms. A MatLAB-based Virtual Phantom for 2D EIT (MatVP2DEIT) is developed to generate accurate 2D boundary data for assessing the 2D EIT inverse solvers and its image reconstruction accuracy. It is a MATLAB-based computer program which defines a phantom domain and its inhomogeneities to generate the boundary potential data by changing its geometric parameters. In MatVP2DEIT, the phantom diameter, domain discretization, inhomogeneity number, inhomogeneity geometry (shape, size and position), electrode geometry, applied current magnitude, current injection pattern, background medium conductivity, inhomogeneity conductivity all are set as the phantom variables and are chosen indipendently for simulating different phantom configurations. A constant current injection is simulated at the phantom boundary with different current injection protocols and boundary potential data are calculated. A number of boundary data sets are generated with different phantom configurations and the resistivity images are reconstructed using EIDORS (Electrical Impedance Tomography and Diffuse Optical Tomography Reconstruction Software). Resistivity images are evaluated with the resistivity parameters and contrast parameters estimated from the elemental resistivity profiles of the reconstructed impedance images. MfMf-EIT system is studied, tested, evaluated with a number of practical phantoms eveloped with non-biological and biological materials and the multifrequency impedance imaging is improved. A number of saline phantoms with single and multiple inhomogeneities are developed and the boundary data profiles are studied and the phantom geometry is modified. NaCl-insulator phantoms and the NaCl-vegetable phantoms with different inhomogeneity configurations are developed and the multifrequency EIT reconstruction is studied with different current patterns, different current amplitudes and different frequencies using EIDORS as well as the developed IIRAs developed in MATLAB to evaluate the phantoms and MfMf-EIT system. Real tissue phantoms are developed with different chicken tissue backgrounds and high resistive inhomogeneities and the resistivity image reconstruction is studied using MfMf-EIT system. Chicken tissue phantoms are developed with chicken muscle tissue (CMTP) paste or chicken tissue blocks (CMTB) as the background mediums and chicken fat tissue, chicken bone, air hole and nylon cylinders are used as the inhomogeneity to obtained different phantom configurations. Resistivity imaging of all the real tissue phantoms is reconstructed in EIDORS and developed IIRAs with different current patterns, different frequencies and the images are evaluated by the image parameters to assess the phantoms as well as the MfMf-EIT system. Gold electrode phantoms are developed with thin film based flexible gold electrode arrays for improved bioimpedance and biomedical imaging. The thin film based gold electrode arrays of high geometric precision are developed on flexible FR4 sheet using electro-deposition process and used as the EIT sensors. The NaCl phantoms and real tissue phantoms are developed with gold electrode arrays and studied with MfMf-EIT system and and the resiulsts are compared with identical stainless steel electrode phantoms. NaCl phantoms are developed with 0.9% NaCl solution with single and multiple insulator or vegetable tissues as inhomogeneity. Gold electrode real tissue phantoms are also developed with chicken muscle tissues and fat tissues or other high resistive objects. The EIT images are reconstructed for the gold electrode NaCl phantoms and the gold electrode real tissue phantoms with different phantom geometries, different inhomogeneity configurations and different current patterns and the results are compared with identical SS electrode phantoms. High speed IIRAs called High Speed Model Based Iterative Image Reconstruction (HSMoBIIR) algorithms are developed in MATLAB for impedance image reconstruction in Electrical Impedance Tomography (EIT) by implementing high speed Jacobian calculation techniques using “Broyden’s Method (BM)” and “Adjoint Broyden’s Method (ABM)”. Gauss Newton method based EIT inverse solvers repeatitively evaluate the Jacobian (J) which consumes a lot of computation time for reconstruction, whereas, the HSMoBIIR with Broyden’s Methods (BM)-based accelerated Jacobian Matrix Calculators (JMCs) provides the high speed schemes for Jacobian (J) computation which is integrated with conjugate gradient scheme (CGS) for fast impedance reconstruction. The Broyden’s method based HSMoBIIR (BM-HSMoBIIR) and Adjoint Broyden’s method based HSMoBIIR (ABM-HSMoBIIR) algorithm are developed for high speed improved impedance imaging using BM based JMC (BM-JMC) and ABM-based JMC (ABM-JMC) respectively. Broyden’s Method based HSMoBIIR algorithms make explicit use of secant and adjoint information that can be obtained from the forward solution of the EIT governing equation and hence both the BM-HSMoBIIR and ABM-HSMoBIIR algorithms reduce the computational time remarkably by approximating the system Jacobian (J) successively through low-rank updates. The impedance image reconstruction is studied with BM-HSMoBIIR and ABM-HSMoBIIR algorithms using the simulated and practical phantom data and results are compared with a Gauss-Newton method based MoBIIR (GNMoBIIR) algorithm. The GNMoBIIR algorithm is developed with a Finite Element Method (FEM) based flexible forward solver (FFS) and Gauss-Newton method based inverse solver (GNIS) working with a modified Newton-Raphson iterative technique (NRIT). FFS solves the forward problem (FP) to obtain the computer estimated boundary potential data (Vc) data and NRIT based GNIS solve the inverse problem (IP) and the conductivity update vector [Δσ] is calculated by conjugate gradient search by comparing Vc measured boundary potential data (Vm) and using the Jacobian (J) matrix computed by the adjoint method. The conductivity reconstruction is studied with GNMoBIIR, BM-HSMoBIIR and ABM-HSMoBIIR algorithms using simulated data a practical phantom data and the results are compared. The reconstruction time, projection error norm (EV) and the solution error norm (Eσ) produced in HSMoBIIR algorithms are calculated and compared with GNMoBIIR algorithm. Results show that both the BM-HSMoBIIR and ABM-HSMoBIIR algorithms successfully reconstructs the conductivity distribution of the domain under test with its proper inhomogeneity and background conductivities for simulation as well as experimental studies. Simulated and practical phantom studies demonstrate that both the BM-HSMoBIIR and ABM-HSMoBIIR algorithms accelerate the impedance reconstruction by more than five times. It is also observed that EV and Eσ are reduced in both the HSMoBIIR algorithms and hence the image quality is improved. Noise analysis and convergence studies show that both the BM-HSMoBIIR and ABM-HSMoBIIR algorithms works faster and better in noisy conditions compared to GNMoBIIR. In low noise conditions, BM-HSMoBIIR is faster than to ABM-HSMoBIIR algorithm. But, in higher noisy environment, the ABM-HSMoBIIR is found faster and better than BM-HSMoBIIR. Two novel regularization methods called Projection Error Propagation-based Regularization (PEPR) and Block Matrix based Multiple Regularization (BMMR) are proposed to improve the image quality in Electrical Impedance Tomography (EIT). PEPR method defines the regularization parameter as a function of the projection error contributed by the mismatch (difference) between the data obtained from the experimental measurements (Vm) and calculated data (Vc). The regularization parameter in the reconstruction algorithm gets modified automatically according to the noise level in measured data and ill-posedness of the Hessian matrix. The L-2 norm of the projection error is calculated using the voltage difference and it is used to find the regularization parameter in each iteration in the reconstruction algorithm. In BMMR method, the response matrix (JTJ) obtained from the Jacobian matrix (J) has been partitioned into several sub-block matrices and the highest eigenvalue of each sub-block matrices has been chosen as regularization parameter for the nodes contained by that sub-block. The BMMR method preserved the local physiological information through the multiple regularization process which is then integrated to the ill-posed inverse problem to make the regularization more effective and optimum for all over the domain. Impedance imaging with simulated data and the practical phantom data is studied with PEPR and BMMR techniques in GNMoBIIR and EIDORS and the reconstructed images are compared with the single step regularization (STR) and Modified Levenberg Regularization (LMR). The projection error and the solution error norms are estimated in the reconstructions processes with PEPR and the BMMR methods and the results are compared with the errors estimated in STR and modified LMR techniques. Reconstructed images obtained with PEPR and BMMR are also studied with image parameters and contrast parameters and the reconstruction performance with PEPR and BMMR are evaluated by comparing the results with STR and modified LMR. PEPR and BMMR techniques are successfully implemented in the GNMoBIIR and EIDORS algorithms to improve the impedance image reconstruction by regularizing the solution domain in EIT reconstruction process. As the multifrequency EIT is always preferred in biological object imaging for better assessments of the frequency dependent bioimpedance response, multifrequency impedance imaging is studied with MfMf-EIT system developed for biomedical applications. MfMf-EIT system is studied, tested and evaluated with practical phantoms suitably developed for multifrequency impedance imaging within a wide range of frequency. Different biological materials are studied with electrical impedance spectroscopy (EIS) and a number of practical biological phantoms suitable for multifrequency EIT imaging are developed. The MfMf-EIT system is studied, tested and evaluated at different frequency levels with different current patterns using a number of NaCl phantoms with single, multiple and hybrid vegetable tissue phantoms as well as with chicken tissue phantoms. BbMfMf-EIT system is also studied and evaluated with the multifrequency EIT imaging using the developed biological phantoms. The developed MfMf-EIT system is applied on human body for impedance imaging of human anatomy. Impedance imaging of human leg and thigh is studied to visualize the muscle and bone tissues using different current patterns and different relative electrode positions. Ag/AgCl electrodes are attached to the leg and thigh using ECG gel and the boundary data are collected with MfMf-EIT EIT system by injecting a 1 mA and 50 kHz sinusoidal constant current with neighbouring and opposite current injection patterns. Impedance images of the femur bone of the human thigh and the tibia and fibula bones of the human leg along with the muscle tissue backgrounds are reconstructed in EIDORS and GNMoBIIR algorithms. Reconstructed resistivity profiles of bone and muscles are compared with the resistivity data profiles reported in the published literature. Impedance imaging of leg and thigh is studied with MfMf-EIT system for different current patterns, relative electrode positions and the images are evaluated to assess the system reliability. Battery based MfMf-EIT system (BbMfMf-EIT) is also studied for human leg and thigh imaging and it is observed that MfMf-EIT system and BbMfMf-EIT system are suitable for impedance imaging of human body imaging though the BbMfMf-EIT system increases the patiet safety. Therefore, the developed MfMf-EIT and BbMfMf-EIT systems are found quite suitable to improve the bio-impedance imaging in medical, biomedical and clinical applications as well as to study the anatomical and physiological status of the human body to diagnose, detect and monitor the tumors, lesions and a number of diseases or anatomical abnormalities in human subjects.

The passive electrical properties of biological tissue have been studied since the 1920s, and with time, the use of Electrical Bioimpedance (EBI) in medicine has successfully spread (Schwan, 1999). Since the electrical properties of tissue are frequency-dependent (Schwan, 1957), observations of the bioimpedance spectrum have created the discipline of Electrical Impedance Spectroscopy (EIS), a discipline that has experienced a development closely related to the progress of electronic instrumentation and the dissemination of EBI technology through medicine.

This report covers results of experiments conducted to obtain data on the fire and deflagration hazards from thermal runaway and its propagation through energy storage systems (ESS). The UL 9540A test standard provides a systematic evaluation of thermal runaway and propagation in energy storage system at cell, module, unit, and installation levels. The data from this testing may be used to design fire and explosion protection systems needed for safe siting and installation of ESS. In addition to temperature, pressure, and gas measurement instruments installed inside of the container, fire service portable gas monitors were placed at locations inside and outside the storage container during the experiments to assess their ability to detect products of thermal runaway and inform fire service size-up decisions. Review section 2.2.3 Fire Service Size-up Equipment to learn more. This research demonstrates a clear need for responding firefighters to have early access to data from instrumentation installed within an ESS, particularly gas measurement instrumentation, available through a monitoring panel. Additionally, it highlights the importance of communication between responding firefighters and personnel responsible for management of the ESS, who can aid in complete evaluation of system data to develop a more clear picture of system status and potential hazards.

Background to the topic: For food safety and security reasons, hundreds of pesticides, veterinary drugs, and environmental pollutants should be monitored in the food supply, but current methods are too time-consuming, laborious, and expensive. As a result, only a tiny fraction of the food is tested for a limited number of contaminants. Original proposal objectives: Our main original goal was to develop fast, practical, and effective new approaches for the analysis of hazardous chemicals in the food supply. We proposed to extend the QuEChERS approach to more pesticides, veterinary drugs and pollutants, further develop GC-MS and LC-MS with SMB and combine QuEChERS with GC-SMB-MS and LC-SMB-EI-MS to provide the “ultimate” approach for the analysis of hazardous chemicals in food. Major conclusions, solutions and achievements: The original QuEChERS method was validated for more than 200 pesticide residues in a variety of food crops. For the few basic pesticides for which the method gave lower recoveries, an extensive solvent suitability study was conducted, and a buffering modification was made to improve results for difficult analytes. Furthermore, evaluation of the QuEChERS approach for fatty matrices, including olives and its oil, was performed. The QuEChERS concept was also extended to acrylamide analysis in foods. Other advanced techniques to improve speed, ease, and effectiveness of chemical residue analysis were also successfully developed and/or evaluated, which include: a simple and inexpensive solvent-in-silicone-tube extraction approach for highly sensitive detection of nonpolar pesticides in GC; ruggedness testing of low-pressure GC-MS for 3-fold faster separations; optimization and extensive evaluation of analyte protectants in GC-MS; and use of prototypical commercial automated direct sample introduction devices for GC-MS. GC-MS with SMB was further developed and combined with the Varian 1200 GCMS/ MS system, resulting in a new type of GC-MS with advanced capabilities. Careful attention was given to the subject of GC-MS sensitivity and its LOD for difficult to analyze samples such as thermally labile pesticides or those with weak or no molecular ions, and record low LOD were demonstrated and discussed. The new approach of electron ionization LC-MS with SMB was developed, its key components of sample vaporization nozzle and flythrough ion source were improved and was evaluated with a range of samples, including carbamate pesticides. A new method and software based on IAA were developed and tested on a range of pesticides in agricultural matrices. This IAA method and software in combination with GC-MS and SMB provide extremely high confidence in sample identification. A new type of comprehensive GCxGC (based on flow modulation) was uniquely combined with GC-MS with SMB, and we demonstrated improved pesticide separation and identification in complex agricultural matrices using this novel approach. An improved device for aroma sample collection and introduction (SnifProbe) was further developed and favorably compared with SPME for coffee aroma sampling. Implications, both scientific and agricultural: We succeeded in achieving significant improvements in the analysis of hazardous chemicals in the food supply, from easy sample preparation approaches, through sample analysis by advanced new types of GC-MS and LCMS techniques, all the way to improved data analysis by lowering LOD and providing greater confidence in chemical identification. As a result, the combination of the QuEChERS approach, new and superior instrumentation, and the novel monitoring methods that were developed will enable vastly reduced time and cost of analysis, increased analytical scope, and a higher monitoring rate. This provides better enforcement, an added impetus for farmers to use good agricultural practices, improved food safety and security, increased trade, and greater consumer confidence in the food supply.