Se connecter

Bibliographies thématiques / Limonoid Biosynthesis / Articles de revues

Pour voir les autres types de publications sur ce sujet consultez le lien suivant : Limonoid Biosynthesis.

Articles de revues sur le sujet « Limonoid Biosynthesis »

Auteur : Grafiati

Publié le 6 septembre 2023

Mis à jour le 19 juillet 2025

Créez une référence correcte selon les styles APA, MLA, Chicago, Harvard et plusieurs autres

Choisissez une source :

Consultez les 43 meilleurs articles de revues pour votre recherche sur le sujet « Limonoid Biosynthesis ».

À côté de chaque source dans la liste de références il y a un bouton « Ajouter à la bibliographie ». Cliquez sur ce bouton, et nous générerons automatiquement la référence bibliographique pour la source choisie selon votre style de citation préféré : APA, MLA, Harvard, Vancouver, Chicago, etc.

Vous pouvez aussi télécharger le texte intégral de la publication scolaire au format pdf et consulter son résumé en ligne lorsque ces informations sont inclues dans les métadonnées.

Parcourez les articles de revues sur diverses disciplines et organisez correctement votre bibliographie.

1

De La Peña, Ricardo, Hannah Hodgson, Jack Chun-Ting Liu, et al. "Complex scaffold remodeling in plant triterpene biosynthesis." Science 379, no. 6630 (2023): 361–68. http://dx.doi.org/10.1126/science.adf1017.

Texte intégral

Résumé :

Triterpenes with complex scaffold modifications are widespread in the plant kingdom. Limonoids are an exemplary family that are responsible for the bitter taste in citrus (e.g., limonin) and the active constituents of neem oil, a widely used bioinsecticide (e.g., azadirachtin). Despite the commercial value of limonoids, a complete biosynthetic route has not been described. We report the discovery of 22 enzymes, including a pair of neofunctionalized sterol isomerases, that catalyze 12 distinct reactions in the total biosynthesis of kihadalactone A and azadirone, products that bear the signature limonoid furan. These results enable access to valuable limonoids and provide a template for discovery and reconstitution of triterpene biosynthetic pathways in plants that require multiple skeletal rearrangements and oxidations.

Styles APA, Harvard, Vancouver, ISO, etc.

2

Hodgson, Hannah, Ricardo De La Peña, Michael J. Stephenson, et al. "Identification of key enzymes responsible for protolimonoid biosynthesis in plants: Opening the door to azadirachtin production." Proceedings of the National Academy of Sciences 116, no. 34 (2019): 17096–104. http://dx.doi.org/10.1073/pnas.1906083116.

Texte intégral

Résumé :

Limonoids are natural products made by plants belonging to the Meliaceae (Mahogany) and Rutaceae (Citrus) families. They are well known for their insecticidal activity, contribution to bitterness in citrus fruits, and potential pharmaceutical properties. The best known limonoid insecticide is azadirachtin, produced by the neem tree (Azadirachta indica). Despite intensive investigation of limonoids over the last half century, the route of limonoid biosynthesis remains unknown. Limonoids are classified as tetranortriterpenes because the prototypical 26-carbon limonoid scaffold is postulated to be formed from a 30-carbon triterpene scaffold by loss of 4 carbons with associated furan ring formation, by an as yet unknown mechanism. Here we have mined genome and transcriptome sequence resources for 3 diverse limonoid-producing species (A. indica, Melia azedarach, and Citrus sinensis) to elucidate the early steps in limonoid biosynthesis. We identify an oxidosqualene cyclase able to produce the potential 30-carbon triterpene scaffold precursor tirucalla-7,24-dien-3β-ol from each of the 3 species. We further identify coexpressed cytochrome P450 enzymes from M. azedarach (MaCYP71CD2 and MaCYP71BQ5) and C. sinensis (CsCYP71CD1 and CsCYP71BQ4) that are capable of 3 oxidations of tirucalla-7,24-dien-3β-ol, resulting in spontaneous hemiacetal ring formation and the production of the protolimonoid melianol. Our work reports the characterization of protolimonoid biosynthetic enzymes from different plant species and supports the notion of pathway conservation between both plant families. It further paves the way for engineering crop plants with enhanced insect resistance and producing high-value limonoids for pharmaceutical and other applications by expression in heterologous hosts.

Styles APA, Harvard, Vancouver, ISO, etc.

3

Pandreka, Avinash, Patil S. Chaya, Ashish Kumar, et al. "Limonoid biosynthesis 3: Functional characterization of crucial genes involved in neem limonoid biosynthesis." Phytochemistry 184 (April 2021): 112669. http://dx.doi.org/10.1016/j.phytochem.2021.112669.

Texte intégral

Styles APA, Harvard, Vancouver, ISO, etc.

4

Pandreka, Avinash, Patil S. Chaya, Ashish Kumar, et al. "Corrigendum to "Limonoid biosynthesis 3: Functional characterization of crucial genes involved in neem limonoid biosynthesis" [Phytochemistry 184 (2021) 112669]." Phytochemistry 187 (April 30, 2021): 112669. https://doi.org/10.1016/j.phytochem.2021.112669.

Texte intégral

Résumé :

Pandreka, Avinash, Chaya, Patil S., Kumar, Ashish, Aarthy, Thiagarayaselvam, Mulani, Fayaj A., Bhagyashree, Date D., Shilpashree, H.B., Jennifer, Cheruvathur, Ponnusamy, Sudha, Nagegowda, Dinesh, Thulasiram, Hirekodathakallu V. (2021): Corrigendum to "Limonoid biosynthesis 3: Functional characterization of crucial genes involved in neem limonoid biosynthesis" [Phytochemistry 184 (2021) 112669]. Phytochemistry (112751) 187: 112669, DOI: 10.1016/j.phytochem.2021.112669, URL: http://dx.doi.org/10.1016/j.phytochem.2021.112669

Styles APA, Harvard, Vancouver, ISO, etc.

5

Herman, Zareb, Chi H. Fong, and Shin Hasegawa. "Biosynthesis of limonoid glucosides in navel orange." Phytochemistry 30, no. 5 (1991): 1487–88. http://dx.doi.org/10.1016/0031-9422(91)84193-v.

Texte intégral

Styles APA, Harvard, Vancouver, ISO, etc.

6

Pandreka, Avinash, Patil S. Chaya, Ashish Kumar, et al. "Corrigendum to “Limonoid biosynthesis 3: Functional characterization of crucial genes involved in neem limonoid biosynthesis” [Phytochemistry 184 (2021) 112669]." Phytochemistry 187 (July 2021): 112751. http://dx.doi.org/10.1016/j.phytochem.2021.112751.

Texte intégral

Styles APA, Harvard, Vancouver, ISO, etc.

7

Fong, Chi H., Shin Hasegawa, Zareb Herman, and Peter Ou. "Biosynthesis of limonoid glucosides in lemon (Citrus limon)." Journal of the Science of Food and Agriculture 54, no. 3 (1991): 393–98. http://dx.doi.org/10.1002/jsfa.2740540310.

Texte intégral

Styles APA, Harvard, Vancouver, ISO, etc.

8

Ou, Peter, Shin Hasegawa, Zareb Herman, and Chi H. Fong. "Limonoid biosynthesis in the stem of Citrus limon." Phytochemistry 27, no. 1 (1988): 115–18. http://dx.doi.org/10.1016/0031-9422(88)80600-9.

Texte intégral

Styles APA, Harvard, Vancouver, ISO, etc.

9

Liu, Cuihua, Min He, Zhuang Wang, and Juan Xu. "Integrative Analysis of Terpenoid Profiles and Hormones from Fruits of Red-Flesh Citrus Mutants and Their Wild Types." Molecules 24, no. 19 (2019): 3456. http://dx.doi.org/10.3390/molecules24193456.

Texte intégral

Résumé :

In citrus color mutants, the levels of carotenoid constituents and other secondary metabolites are different in their corresponding wild types. Terpenoids are closely related to coloration, bitterness, and flavor. In this study, terpenoid profiles and hormones in citrus fruits of two red-flesh mutants—Red Anliu orange and Red-flesh Guanxi pummelo—and their corresponding wild types were investigated using GC/MS, HPLC, and LC-MS/MS. Results showed that Red Anliu orange (high in carotenoids) and Anliu orange (low in carotenoids) accumulated low levels of limonoid aglycones but high levels of monoterpenoids; conversely, Red-flesh Guanxi pummelo (high in carotenoids) and Guanxi pummelo (deficient in carotenoids) accumulated high levels of limonoid aglycones but low levels of monoterpenoids. However, isopentenyl diphosphate was present at similar levels. A correlation analysis indicated that jasmonic and salicylic acids might play important roles in regulating terpenoid biosynthesis. Additionally, the similarities of carotenoid and volatile profiles between each mutant and its corresponding wild type were greater than those between the two mutants or the two wild types. The flux balance of terpenoid metabolism in citrus fruit tends toward stability among various citrus genera that have different terpenoid profiles. Bud mutations could influence metabolite profiles of citrus fruit to a limited extent.

Styles APA, Harvard, Vancouver, ISO, etc.

10

Hullin-Matsuda, Françoise, Nario Tomishige, Shota Sakai, et al. "Limonoid Compounds Inhibit Sphingomyelin Biosynthesis by Preventing CERT Protein-dependent Extraction of Ceramides from the Endoplasmic Reticulum." Journal of Biological Chemistry 287, no. 29 (2012): 24397–411. http://dx.doi.org/10.1074/jbc.m112.344432.

Texte intégral

Styles APA, Harvard, Vancouver, ISO, etc.

11

Li, Wanshan, Li Shen, Torsten Bruhn, Patchara Pedpradab, Jun Wu, and Gerhard Bringmann. "Trangmolins A-F with an Unprecedented Structural Plasticity of the Rings A and B: New Insight into Limonoid Biosynthesis." Chemistry - A European Journal 22, no. 33 (2016): 11719–27. http://dx.doi.org/10.1002/chem.201602230.

Texte intégral

Styles APA, Harvard, Vancouver, ISO, etc.

12

Zhang, Xiaoyue, Qinyang Song, Hanghang Zheng, Rui Wang, and Qiang Zhang. "Toxicity and Metabolomic Dysfunction Invoked by Febrifugin, a Harmful Component of Edible Nut of Swietenia macrophylla." International Journal of Molecular Sciences 25, no. 17 (2024): 9753. http://dx.doi.org/10.3390/ijms25179753.

Texte intégral

Résumé :

Swietenia macrophylla fruit is a valuable and historically significant medicinal plant with anti-hypertension and anti-diabetes. We identified a toxic component, Febrifugin, from the edible part of the nut following zebrafish toxicity-guided isolation. Febrifugin is a mexicanolide-type limonoid compound. The toxic factor induced acute toxicity in zebrafish, including yolk sac edema and pericardial edema, reduced body length, decreased melanin deposition, and presented acute skeletal developmental issues. Further exploration of the acute toxicity mechanism through metabolomics revealed that Febrifugin caused significant changes in 13 metabolites in zebrafish larvae, which are involved in the pentose phosphate, tricarboxylic acid (TCA) cycle, and amino acid biosynthesis. The bioassay of oxidative stress capacity and qRT-PCR measurement showed that the compound significantly affected the h6pd gene in the pentose phosphate pathway and the mRNA expression of cs, idh3a, fh, and shda genes in the TCA cycle, leading to reactive oxygen species (ROS) accumulation and a notable decrease in glutathione (GSH) activity in zebrafish. These findings provide a basis for the rational use of S. macrophylla as a medicinal plant and raise awareness of the safety of medicinal plants.

Styles APA, Harvard, Vancouver, ISO, etc.

13

Li, Wan-Shan, Attila Mándi, Jun-Jun Liu, Li Shen, Tibor Kurtán, and Jun Wu. "Xylomolones A–D from the Thai Mangrove Xylocarpus moluccensis: Assignment of Absolute Stereostructures and Unveiling a Convergent Strategy for Limonoid Biosynthesis." Journal of Organic Chemistry 84, no. 5 (2019): 2596–606. http://dx.doi.org/10.1021/acs.joc.8b03037.

Texte intégral

Styles APA, Harvard, Vancouver, ISO, etc.

14

Tsamo, Armelle Tontsa, Julio Issah Mawouma Pagna, Pamela Kemda Nangmo, Pierre Mkounga, Hartmut Laatsch, and Augustin Ephrem Nkengfack. "Rubescins F–H, new vilasinin-type limonoids from the leaves of Trichilia rubescens (Meliaceae)." Zeitschrift für Naturforschung C 74, no. 7-8 (2019): 175–82. http://dx.doi.org/10.1515/znc-2018-0187.

Texte intégral

Résumé :

Abstract Three new limonoids, designated as rubescins F (1), G (2), and H (3), together with two known compounds of this type, TS1 (4) and trichirubine A (5), were isolated from methylene chloride/methanol extracts of Trichilia rubescens leaves. The structures of these compounds were elucidated based on 1D and 2D nuclear magnetic resonance (NMR) analysis and complemented by electrospray ionization high-resolution mass spectrometry results and by comparison to data of related compounds described in the literature and ab initio calculations. Rubescin F (1) is the first limonoid from Trichilia spp. with an oxetane ring between C-7 and C-14, which seems to be formed by the isomerization of TS1 (4). The γ-hydroxybutenolide rubescin G (2) is a potential precursor of trichirubine A (5), whereas rubescin H (3) is the first example of a triterpenoid with a single bond between C-7/C-14, forming a cyclopropane ring. The absolute configuration of these limonoids was derived from biosynthetic considerations and ab initio calculations of NMR and optical rotation dispersion data.

Styles APA, Harvard, Vancouver, ISO, etc.

15

Narender, Tadigoppula, Tanvir Khaliq, Shweta, Kancharla P. Reddy, and Ravi K. Sharma. "Occurrence, Biosynthesis, Biological activity and NMR Spectroscopy of D and B, D Ring Seco-limonoids of Meliaceae Family." Natural Product Communications 2, no. 2 (2007): 1934578X0700200. http://dx.doi.org/10.1177/1934578x0700200219.

Texte intégral

Résumé :

Limonoids are modified tetranortriterpenoids, classified on the basis of which of the four rings (A, B, C and D) in the intact triterpene nucleus have been oxidized. The order Rutales produces a variety of seco-limonoids, such as A, B, C, D, AB, AD, and BD-ring seco-limonoids. The Meliaceae family, belonging to the order Rutales, has yielded several D-ring and B, D-ring seco-limonoids This review describes the occurrence, biosynthesis, biological activity and NMR spectroscopy of D ring seco-limonoids, such as gedunin derivatives and B, D-ring seco-limonoids, such as methyl angolensates, xyloccensins, methyl meliacates, phragmalins and modified phragmalins. The literature from 1990 to 2005 is reviewed.

Styles APA, Harvard, Vancouver, ISO, etc.

16

Hashinaga, Fumio, Chi H. Fong, and Shin Hasegawa. "Biosynthesis of Limonoids inCitrus sudachi." Agricultural and Biological Chemistry 54, no. 11 (1990): 3019–20. http://dx.doi.org/10.1080/00021369.1990.10870416.

Texte intégral

Styles APA, Harvard, Vancouver, ISO, etc.

17

HASHINAGA, Fumio, Chi H. FONG, and Shin HASEGAWA. "Biosynthesis of limonoids in Citrus sudachi." Agricultural and Biological Chemistry 54, no. 11 (1990): 3019–20. http://dx.doi.org/10.1271/bbb1961.54.3019.

Texte intégral

Styles APA, Harvard, Vancouver, ISO, etc.

18

Zhou, Yu, Yuxiang Zhang, Detian Mu, et al. "Selection of Reference Genes in Evodia rutaecarpa var. officinalis and Expression Patterns of Genes Involved in Its Limonin Biosynthesis." Plants 12, no. 18 (2023): 3197. http://dx.doi.org/10.3390/plants12183197.

Texte intégral

Résumé :

E. rutaecarpa var. officinalis is a traditional Chinese medicinal plant known for its therapeutic effects, which encompass the promotion of digestion, the dispelling of cold, the alleviation of pain, and the exhibition of anti-inflammatory and antibacterial properties. The principal active component of this plant, limonin, is a potent triterpene compound with notable pharmacological activities. Despite its significance, the complete biosynthesis pathway of limonin in E. rutaecarpa var. officinalis remains incompletely understood, and the underlying molecular mechanisms remain unexplored. The main purpose of this study was to screen the reference genes suitable for expression analysis in E. rutaecarpa var. officinalis, calculate the expression patterns of the genes in the limonin biosynthesis pathway, and identify the relevant enzyme genes related to limonin biosynthesis. The reference genes play a pivotal role in establishing reliable reference standards for normalizing the gene expression data, thereby ensuring precision and credibility in the biological research outcomes. In order to identify the optimal reference genes and gene expression patterns across the diverse tissues (e.g., roots, stems, leaves, and flower buds) and developmental stages (i.e., 17 July, 24 August, 1 September, and 24 October) of E. rutaecarpa var. officinalis, LC-MS was used to analyze the limonin contents in distinct tissue samples and developmental stages, and qRT-PCR technology was employed to investigate the expression patterns of the ten reference genes and eighteen genes involved in limonin biosynthesis. Utilizing a comprehensive analysis that integrated three software tools (GeNorm ver. 3.5, NormFinder ver. 0.953 and BestKeeper ver. 1.0) and Delta Ct method alongside the RefFinder website, the best reference genes were selected. Through the research, we determined that Act1 and UBQ served as the preferred reference genes for normalizing gene expression during various fruit developmental stages, while Act1 and His3 were optimal for different tissues. Using Act1 and UBQ as the reference genes, and based on the different fruit developmental stages, qRT-PCR analysis was performed on the pathway genes selected from the “full-length transcriptome + expression profile + metabolome” data in the limonin biosynthesis pathway of E. rutaecarpa var. officinalis. The findings indicated that there were consistent expression patterns of HMGCR, SQE, and CYP450 with fluctuations in the limonin contents, suggesting their potential involvement in the limonin biosynthesis of E. rutaecarpa var. officinalis. This study lays the foundation for further research on the metabolic pathway of limonin in E. rutaecarpa var. officinalis and provides reliable reference genes for other researchers to use for conducting expression analyses.

Styles APA, Harvard, Vancouver, ISO, etc.

19

Hasegawa, Shin, Zareb Herman, Ed Orme, and Peter Ou. "Biosynthesis of limonoids in Citrus: Sites and translocation." Phytochemistry 25, no. 12 (1986): 2783–85. http://dx.doi.org/10.1016/s0031-9422(00)83741-3.

Texte intégral

Styles APA, Harvard, Vancouver, ISO, etc.

20

Jin, Jie, Xinhuang Lv, Ben Wang та ін. "Limonin Inhibits IL-1β-Induced Inflammation and Catabolism in Chondrocytes and Ameliorates Osteoarthritis by Activating Nrf2". Oxidative Medicine and Cellular Longevity 2021 (9 листопада 2021): 1–15. http://dx.doi.org/10.1155/2021/7292512.

Texte intégral

Résumé :

Osteoarthritis (OA), a degenerative disorder, is considered to be one of the most common forms of arthritis. Limonin (Lim) is extracted from lemons and other citrus fruits. Limonin has been reported to have anti-inflammatory effects, while inflammation is a major cause of OA; thus, we propose that limonin may have a therapeutic effect on OA. In this study, the therapeutic effect of limonin on OA was assessed in chondrocytes in vitro in IL-1β induced OA and in the destabilization of the medial meniscus (DMM) mice in vivo. The Nrf2/HO-1/NF-κB signaling pathway was evaluated to illustrate the working mechanism of limonin on OA in chondrocytes. In this study, it was found that limonin can reduce the level of IL-1β induced proinflammatory cytokines such as INOS, COX-2, PGE2, NO, TNF-α, and IL-6. Limonin can also diminish the biosynthesis of IL-1β-stimulated chondrogenic catabolic enzymes such as MMP13 and ADAMTS5 in chondrocytes. The research on the mechanism study demonstrated that limonin exerts its protective effect on OA through the Nrf2/HO-1/NF-κB signaling pathway. Taken together, the present study shows that limonin may activate the Nrf2/HO-1/NF-κB pathway to alleviate OA, making it a candidate therapeutic agent for OA.

Styles APA, Harvard, Vancouver, ISO, etc.

21

Hasegawa, Shin, and Zareb Herman. "Biosynthesis of limonoids: Conversion of deacetylnomilinate to nomilin in Citrus limon." Phytochemistry 25, no. 11 (1986): 2523–24. http://dx.doi.org/10.1016/s0031-9422(00)84500-8.

Texte intégral

Styles APA, Harvard, Vancouver, ISO, etc.

22

Rodríguez Ceraolo, Cecilia, Valeria Vázquez, Ignacio Migues, María Verónica Cesio, Fernando Rivas, and Horacio Heinzen. "Flavonoids and Limonoids Profiles Variation in Leaves from Mandarin Cultivars and Its Relationship with Alternate Bearing." Agronomy 12, no. 1 (2022): 121. http://dx.doi.org/10.3390/agronomy12010121.

Texte intégral

Résumé :

Alternate bearing in citrus trees has been extensively studied as a key feature for citrus growers. Although the genetic and the biochemical process occurring during alternate bearing has been studied extensively, there is a lack of information identifying the presence of metabolic indicators during “on” and “off” years. In citrus plants, leaves play a central role in the metabolic pathway triggering the flowering induction process. To investigate the changes during this transition, a liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) analysis of the leaf profiles of 20 compounds (17 polyphenols, two limonoids, and one furanocoumarin), in bearing and non-bearing branches arising from four different mandarin genotypes, was performed. The same metabolites were found in all the genotypes at both stages: both limonoids and 11 polyphenols. Using these compounds, the chemotaxonomic differentiation between cultivars was assessed. The levels of flavanones and limonoids showed differences in both bearing stages and the transition from vegetative to flowering could be shown by the activation of the polyphenol biosynthetic pathway, from precursors like naringenin to metabolic end-points such as narirutin and polymethoxyflavones. Narirutin levels showed significant differences between both stages, suggesting it as a possible marker of the physiological status of the branch.

Styles APA, Harvard, Vancouver, ISO, etc.

23

Herman, Z. "Limonin biosynthesis from obacunone via obacunoate in Citrus limon." Phytochemistry 23, no. 12 (1985): 2911–13. http://dx.doi.org/10.1016/s0031-9422(00)80603-2.

Texte intégral

Styles APA, Harvard, Vancouver, ISO, etc.

24

Herman, Zareb, and Shin Hasegawa. "Limonin biosynthesis from obacunone via obacunoate in Citrus limon." Phytochemistry 24, no. 12 (1985): 2911–13. http://dx.doi.org/10.1016/0031-9422(85)80025-x.

Texte intégral

Styles APA, Harvard, Vancouver, ISO, etc.

25

Su, Xinyao, Zhipeng Liang, Qiang Xue, Jia Liu, Xuemi Hao, and Caixia Wang. "A comprehensive review of azadirachtin: physicochemical properties, bioactivities, production, and biosynthesis." Acupuncture and Herbal Medicine 3, no. 4 (2023): 256–70. http://dx.doi.org/10.1097/hm9.0000000000000086.

Texte intégral

Résumé :

Azadirachtin, a complex tetratriterpenoid limonin with potent insecticidal properties, is the most widely used biological pesticide worldwide. Its versatile pharmacological applications include the inhibition of tumor growth and anti-malarial, anti-bacterial, and anti-inflammatory properties. Azadirachtin plays a pivotal role in pest control and novel drug development. The primary source of azadirachtin is the neem tree (Azadirachta indica A. Juss), with an azadirachtin content ranging from 0.3% to 0.5%. Despite the market demand for botanical pesticides reaching approximately 100,000 tons per year, the annual neem production in China is only 1.14 tons. Although azadirachtin can be obtained through plant extraction or chemical synthesis, the quantity obtained does not meet the market demand in China. The sluggish pace of azadirachtin biosynthesis results from the limited availability of genetic information and the complexity of the synthetic pathway. Recent advancements in azadirachtin biosynthesis hold promise as an efficient collection method. In this study, we explored the physicochemical properties, biological activities, mechanisms of action, and acquisition methods of azadirachtin. We also delved into recent progress in azadirachtin biosynthesis and assessed potential future usage challenges. This study aims to establish a theoretical foundation for the scientific application and efficient synthesis of azadirachtin, offering valuable reference information to the industry.

Styles APA, Harvard, Vancouver, ISO, etc.

26

Hu, Wei-Min, and Jun Wu. "Protoxylogranatin B, a Key Biosynthetic Intermediate from Xylocarpus granatum: Suggesting an Oxidative Cleavage Biogenetic Pathway to Limonoid." Open Natural Products Journal 3, no. 1 (2010): 1–5. http://dx.doi.org/10.2174/1874848101003010001.

Texte intégral

Styles APA, Harvard, Vancouver, ISO, etc.

27

Izumi, Yuriko, Eri Kamei, Yoko Miyamoto, et al. "Role of the Pathotype-Specific ACRTS1 Gene Encoding a Hydroxylase Involved in the Biosynthesis of Host-Selective ACR-Toxin in the Rough Lemon Pathotype of Alternaria alternata." Phytopathology® 102, no. 8 (2012): 741–48. http://dx.doi.org/10.1094/phyto-02-12-0021-r.

Texte intégral

Résumé :

The rough lemon pathotype of Alternaria alternata produces host-selective ACR-toxin and causes Alternaria leaf spot disease of the rootstock species rough lemon (Citrus jambhiri) and Rangpur lime (C. limonia). Genes controlling toxin production were localized to a 1.5-Mb chromosome carrying the ACR-toxin biosynthesis gene cluster (ACRT) in the genome of the rough lemon pathotype. A genomic BAC clone containing a portion of the ACRT cluster was sequenced which allowed identification of three open reading frames present only in the genomes of ACR-toxin producing isolates. We studied the functional role of one of these open reading frames, ACRTS1 encoding a putative hydroxylase, in ACR-toxin production by homologous recombination-mediated gene disruption. There are at least three copies of ACRTS1 gene in the genome and disruption of two copies of this gene significantly reduced ACR-toxin production as well as pathogenicity; however, transcription of ACRTS1 and production of ACR-toxin were not completely eliminated due to remaining functional copies of the gene. RNA-silencing was used to knock down the remaining ACRTS1 transcripts to levels undetectable by reverse transcription-polymerase chain reaction. The silenced transformants did not produce detectable ACR-toxin and were not pathogenic. These results indicate that ACRTS1 is an essential gene in ACR-toxin biosynthesis in the rough lemon pathotype of A. alternata and is required for full virulence of this fungus.

Styles APA, Harvard, Vancouver, ISO, etc.

28

Vasquez‐Ruiz, Vianey, M. Ángeles Ramírez‐Cisneros, and Maria Yolanda Rios. "Triterpenes and limonoids of Cedrela : Distribution, biosynthesis, and ¹ H and ¹³ C NMR data." Magnetic Resonance in Chemistry 60, no. 3 (2021): 275–358. http://dx.doi.org/10.1002/mrc.5229.

Texte intégral

Styles APA, Harvard, Vancouver, ISO, etc.

29

Villa-Ruano, Nemesio, Luis Ángel Morales-Mora, Jenaro Leocadio Varela-Caselis, Antonio Rivera, María de los Ángeles Valencia de Ita, and Omar Romero-Arenas. "Arcopilus aureus MaC7A as a New Source of Resveratrol: Assessment of Amino Acid Precursors, Volatiles, and Fungal Enzymes for Boosting Resveratrol Production in Batch Cultures." Applied Sciences 11, no. 10 (2021): 4583. http://dx.doi.org/10.3390/app11104583.

Texte intégral

Résumé :

The chemical factors that regulate the synthesis of resveratrol (RV) in filamentous fungi are still unknown. This work reports on the RV production by Arcopilus aureus MaC7A under controlled conditions and the effect of amino acid precursors (PHE and TYR), monoterpenes (limonone, camphor, citral, thymol, menthol), and mixtures of hydrolytic enzymes (Glucanex) as elicitors for boosting fungal RV. Batch cultures with variable concentrations of PHE and TYR (50–500 mg L−1) stimulated RV production from 127.9 ± 4.6 to 221.8 ± 5.2 mg L−1 in basic cultures developed in PDB (pH 7) added with 10 g L−1 peptone at 30 °C. Maximum levels of RV and biomass were maintained during days 6–8 under these conditions, whereas a dramatic RV decrease was observed from days 10–12 without any loss of biomass. Among the tested volatiles, citral (50 mg L−1) enhanced RV production until 187.8 ± 2.2 mg L−1 in basic cultures, but better results were obtained with Glucanex (100 mg L−1; 198.3 ± 7.6 mg L−1 RV). Optimized batch cultures containing TYR (200 mg L−1), citral (50 mg L−1), thymol (50 mg L−1), and Glucanex (100 mg L−1) produced up to 237.6 ± 4.7 mg L−1 of RV. Our results suggest that low concentrations of volatiles and mixtures of isoenzymes with β-1, 3 glucanase activity increase the biosynthesis of fungal RV produced by A. aureus MaC7A in batch cultures.

Styles APA, Harvard, Vancouver, ISO, etc.

30

Washington, Taylor L., Fabiana V. Briceno, Charles A. Sims, Katlyn Nau, Yavuz Yagiz, and Liwei Gu. "Macroporous adsorbent resin debittering of Huang long bing (HLB)‐affected orange juice and its impacts on consumer sensory acceptance." Journal of Food Science 90, no. 2 (2025). https://doi.org/10.1111/1750-3841.70048.

Texte intégral

Résumé :

AbstractHuang long bing (HLB) infection of oranges induces the biosynthesis and accumulation of bitter limonoids. The objective of this study was to debitter HLB‐affected orange juice while preserving the tasteless and health‐promoting limonoid glucoside and flavanones using resin adsorption. Three resins (FPX66, PAD900, and XAD16N) were found to have higher adsorption and desorption capacity for limonin among seven selected resins. Adsorption of limonoids rapidly increased in the first 2 h of kinetic tests, while slower adsorption kinetics were observed for flavanones. Limonin isothermal adsorption fit best with the Langmuir model, suggesting a physical, monolayer process. Dynamic adsorption showed that a fixed‐bed column packed with PAD900 was able to debitter 200 bed volumes of orange juice before limonin reached its taste threshold of 4.7 µg/mL in the eluent. The resin was regenerated by eluting the column with four bed volumes of alcohol or 4% NaOH to remove adsorbed compounds from the resin. Resin adsorption reduced limonin content in orange juice from 5.85 to 1.34 µg/mL but also decreased tasteless compounds by at least 40%. A consumer sensory test gave the debittered juice a higher overall liking and preference over untreated juice. This study showed that resin adsorption is an effective approach to reduce the bitterness of orange juice affected by HLB.

Styles APA, Harvard, Vancouver, ISO, etc.

31

"Citrus Limonoid Glucosyltransferase: AKey Player For Natural Debittering And Anticancerous Potential." Archives of Life Science and Nurtitional Research, November 28, 2017, 1–16. http://dx.doi.org/10.31829/2765-8368/alsnr2017-1(1)-101.

Texte intégral

Résumé :

Citrus fruits and juices are rich source of health benefitting phytochemicals which play a vital role in balanced diet and disease prevention. Citrus limonoids and flavonoids are the major phytochemicals which are of great interest in pharmaceutical industries because of their demonstrated anticancerous, antioxidant, anti-inflammatory, hormonal stimulation, antibacterial and antiviral actions. Citrus limonoid biosynthetic pathway contains an important regulatory limonoid glucosyltransferase enzyme (LGT). LGT is the natural debittering enzyme encoded by a single copy gene which has been isolated from different Citrus spp. This enzyme is mainly responsible for conversion of all limonoid aglycones (mostly bitter) to their corresponding glucosides (mostly nonbitter) but only during late fruit developmental stage of citrus. Citrus LGT belongs to glycosyltransferase super family whose members are the wide managers to catalyze the transfer of sugar molecules to their acceptor molecules to play several key modifications in plant secondary metabolites. These reveal great significance value in plant cell metabolism especially in detoxification of xenobiotics, production and storage of natural products. Despite to the fact that over expression of LGT in citrus will lead to reduce the delayed bitterness caused by limonin (an aglycone) but in addition will enhance the accumulation of limonoid glucosides in fruits. Further, recent studies suggest that citrus limonoids especially glucosides have shown importance against brain, pancreas, colon, and breast cancers. Thus, future studies should be focused on utilizing the potential of LGT present in citrus plants in terms of anticancerous properties as well as reducing the delayed bitterness problem important for citrus juice industry

Styles APA, Harvard, Vancouver, ISO, etc.

32

Yu, Fang, Babu Gajendran, Ning Wang, et al. "ERK activation via A1542/3 limonoids attenuates erythroleukemia through transcriptional stimulation of cholesterol biosynthesis genes." BMC Cancer 21, no. 1 (2021). http://dx.doi.org/10.1186/s12885-021-08402-6.

Texte intégral

Résumé :

Abstract Background Cholesterol plays vital roles in human physiology; abnormal levels have deleterious pathological consequences. In cancer, elevated or reduced expression of cholesterol biosynthesis is associated with good or poor prognosis, but the underlying mechanisms are largely unknown. The limonoid compounds A1542 and A1543 stimulate ERK/MAPK by direct binding, leading to leukemic cell death and suppression of leukemia in mouse models. In this study, we investigated the downstream consequences of these ERK/MAPK agonists in leukemic cells. Methods We employed RNAseq analysis combined with Q-RT-PCR, western blot and bioinformatics to identify and confirm genes whose expression was altered by A1542 and A1543 in leukemic cells. ShRNA lentiviruses were used to silence gene expression. Cell culture and an animal model (BALB/c) of erythroleukemia induced by Friend virus were utilized to validate effects of cholesterol on leukemia progression. Results RNAseq analysis of A1542-treated cells revealed the induction of all 18 genes implicated in cholesterol biosynthesis. Expression of these cholesterol genes was blocked by cedrelone, an ERK inhibitor. The cholesterol inhibitor lovastatin diminished ERK/MAPK activation by A1542, thereby reducing leukemic cell death induced by this ERK1/2 agonist. Growth inhibition by cholesterol was observed both at the intracellular level, and when orally administrated into a leukemic mouse model. Both HDL and LDL also suppressed leukemogenesis, implicating these lipids as important prognostic markers for leukemia progression. Mechanistically, knockdown experiments revealed that the activation of SREBP1/2 by A1542-A1543 was responsible for induction of only a sub-set of cholesterol biosynthesis genes. Induction of other regulatory factors by A1542-A1543 including EGR1, AP1 (FOS + JUN) LDLR, IER2 and others may cooperate with SREBP1/2 to induce cholesterol genes. Indeed, pharmacological inhibition of AP1 significantly inhibited cholesterol gene expression induced by A1542. In addition to leukemia, high expression of cholesterol biosynthesis genes was found to correlate with better prognosis in renal cancer. Conclusions This study demonstrates that ERK1/2 agonists suppress leukemia and possibly other types of cancer through transcriptional stimulation of cholesterol biosynthesis genes.

Styles APA, Harvard, Vancouver, ISO, etc.

33

Hodgson, Hannah, Michael J. Stephenson, Shingo Kikuchi, et al. "Plants Utilize a Protection/Deprotection Strategy in Limonoid Biosynthesis: A “Missing Link” Carboxylesterase Boosts Yields and Provides Insights into Furan Formation." Journal of the American Chemical Society, October 17, 2024. http://dx.doi.org/10.1021/jacs.4c11213.

Texte intégral

Styles APA, Harvard, Vancouver, ISO, etc.

34

Chuang, Ling, Shenyu Liu, Dave Biedermann, and Jakob Franke. "Identification of early quassinoid biosynthesis in the invasive tree of heaven (Ailanthus altissima) confirms evolutionary origin from protolimonoids." Frontiers in Plant Science 13 (August 23, 2022). http://dx.doi.org/10.3389/fpls.2022.958138.

Texte intégral

Résumé :

The tree of heaven, Ailanthus altissima (MILL.) SWINGLE, is a globally invasive plant known to secrete allelopathic metabolites called quassinoids. Quassinoids are highly modified triterpenoids. So far, nothing has been known about the biochemical basis of quassinoid biosynthesis. Here, based on transcriptome and metabolome data of Ailanthus altissima, we present the first three steps of quassinoid biosynthesis, which are catalysed by an oxidosqualene cyclase and two cytochrome P450 monooxygenases, resulting in the formation of the protolimonoid melianol. Strikingly, these steps are identical to the first steps of the biosynthesis of limonoids, structurally different triterpenoids from sister plant families within the same order Sapindales. Our results are therefore not only important to fully understand the biosynthesis of complex triterpenoids in plants, but also confirm the long-standing hypothesis that quassinoids and limonoids share an evolutionary origin. In addition, our transcriptome data for Ailanthus altissima will be beneficial to other researchers investigating the physiology and ecology of this invasive tree.

Styles APA, Harvard, Vancouver, ISO, etc.

35

Zhang, Pan, Xiaofeng Liu, Xin Yu, et al. "The MYB transcription factor CiMYB42 regulates limonoids biosynthesis in citrus." BMC Plant Biology 20, no. 1 (2020). http://dx.doi.org/10.1186/s12870-020-02475-4.

Texte intégral

Styles APA, Harvard, Vancouver, ISO, etc.

36

Cui, Gaofeng, Yun Li, Xin Yi, et al. "Meliaceae genomes provide insights into wood development and limonoids biosynthesis." Plant Biotechnology Journal, December 2022. http://dx.doi.org/10.1111/pbi.13973.

Texte intégral

Styles APA, Harvard, Vancouver, ISO, etc.

37

Su, Jianmu, Mingmin Jiang, Huimin Pan, et al. "Multi-omics analyses reveal the effects of layerage and grafting on flavonoid synthesis and accumulation in Citrus reticulata ‘Chachi’." Horticulture Research, July 7, 2025. https://doi.org/10.1093/hr/uhaf177.

Texte intégral

Résumé :

Abstract Guangdong Citri Reticulatae Pericarpium from the dry and mature peel of Citrus reticulata ‘Chachi’ (CRC) is a well-known medicinal and food materials in Asia. The main propagation methods of CRC are layerage and grafting. It is generally considered that the quality of CRC from layerage is superior to that obtained from plants propagated by grafting. Nevertheless, the effects of layerage and grafting on main bioactive ingredients flavonoid biosynthesis in peel of CRC keep unknown. Here, metabolomic analyses revealed the effects of layerage, self-grafting and heterografting (C. limonia as rootstock) on flavonoid biosynthesis in CRC from two main harvesting periods, CRCV (Citri Reticulatae Chachiensis Viride) and CRCR (Citri Reticulatae Chachiensis Reddish). Compared with CRCR, CRCV exhibited a higher content of flavonoids. Grafting CRC onto C. limonia exhibited a higher content of hesperidin, nobiletin, tangeretin, narirutin, demethylnobiletin and sinensetin, than layerage and self-grafting. This increase can be attributed to the upregulation of genes involved in flavonoid synthesis. Further, the transcription factor CrcMYBF1 was identified within the gene co-expression network and is confirmed to be significantly induced by methyl jasmonate (MeJA) and up-regulate the expression of Crc1,6RhaT through interacting with its promoter region, thereby boosting the biosynthesis and accumulation of hesperidin. In summary, our findings provide mechanistic insights into the coordinated regulation of hesperidin biosynthesis via MeJA inducing CrcMYBF1 in CRC. Our study is expected to provide a theoretical basis for CRC propagation and cultivation.

Styles APA, Harvard, Vancouver, ISO, etc.

38

Zhang, Pan, Xiaofeng Liu, Xin Yu, et al. "Correction to: The MYB transcription factor CiMYB42 regulates limonoids biosynthesis in citrus." BMC Plant Biology 20, no. 1 (2020). http://dx.doi.org/10.1186/s12870-020-02491-4.

Texte intégral

Styles APA, Harvard, Vancouver, ISO, etc.

39

Wang, Fusheng, Mei Wang, Xiaona Liu, et al. "Identification of Putative Genes Involved in Limonoids Biosynthesis in Citrus by Comparative Transcriptomic Analysis." Frontiers in Plant Science 8 (May 12, 2017). http://dx.doi.org/10.3389/fpls.2017.00782.

Texte intégral

Styles APA, Harvard, Vancouver, ISO, etc.

40

Mahur, Pragati, Abhishek Sharma, Amit Kumar Singh, Jayaraman Muthukumaran, and Monika Jain. "Computational Exploration of Limonin as a Potential Inhibitor of DapB in Klebsiella pneumoniae." Chemistry & Biodiversity, October 2024. http://dx.doi.org/10.1002/cbdv.202402053.

Texte intégral

Résumé :

Klebsiella pneumoniae has emerged as a significant multidrug‐resistant pathogen, classified as a critical priority by the World Health Organization. The rising rates of antibiotic resistance have led to increased therapeutic failures, diminishing the effectiveness of existing antibiotics. Consequently, there is an urgent need for alternative treatments to effectively inhibit the growth of K. pneumoniae and mitigate associated diseases. Phytochemicals have demonstrated potential advantages over traditional antibiotics, prompting their exploration as innovative therapeutic agents. This study aimed to identify phytochemicals that can inhibit dapB, a vital enzyme in the lysine biosynthesis pathway of K. pneumoniae, which is essential for protein synthesis and the cross‐linking of the bacterial peptidoglycan cell wall. We screened 17,934 phytochemicals based on Lipinski’s Rule of Five, along with their Absorption, Distribution, Metabolism, Excretion properties and toxicological parameters. Next, we conducted triplicate docking studies against dapB to evaluate the library further. The most promising molecules then underwent 100 ns Molecular Dynamics simulations in triplicate, followed by binding free energy calculations to identify potential dapB inhibitors. This in silico analysis highlighted limonin as a promising inhibitor of dapB in K. pneumoniae. Further experimental validation is crucial to enhance limonin’s potential as a novel therapeutic agent against K. pneumoniae‐associated diseases.

Styles APA, Harvard, Vancouver, ISO, etc.

41

Lu, Yingying, Huimin Liang, Jialin Liao, et al. "Chromosome-scale assembly and analysis of yellow Camellia (Camellia limonia) genome reveal plant adaptation mechanism and flavonoid biosynthesis in karst region." Global Ecology and Conservation, November 2024, e03296. http://dx.doi.org/10.1016/j.gecco.2024.e03296.

Texte intégral

Styles APA, Harvard, Vancouver, ISO, etc.

42

Aarthy, Thiagarayaselvam, Fayaj A. Mulani, Avinash Pandreka, et al. "Tracing the biosynthetic origin of limonoids and their functional groups through stable isotope labeling and inhibition in neem tree (Azadirachta indica) cell suspension." BMC Plant Biology 18, no. 1 (2018). http://dx.doi.org/10.1186/s12870-018-1447-6.

Texte intégral

Styles APA, Harvard, Vancouver, ISO, etc.

43

"Book reviews: Citrus Limonoids: Functional Chemicals in Agriculture and Food, ed. Mark A. Berhow, Shin Hasegawa and Gary D. Manners (reviewed by Robert A. Hill); Biosynthesis: Polyketides and Vitamins, ed. F. J. Leeper and J. C. Vederas (reviewed by Dr Alison Hill); Biosynthesis: Aromatic Polyketides, Isoprenoids and Alkaloids, F. J. Leeper and J. C. Vederas (reviewed by T. J. Simpson); Pharmaceuticals: Classes, Therapeutic Agents, Areas of Application, ed. J. L. McGuire (reviewed by Barrie Wilkinson); Medicinal Plants of the World: Chemical Constituents, Traditional and Modern Medicinal Uses. Vol. 2, Ivan A. Ross (reviewed by Thomas Hemscheidt); Amino Acids, Peptides and Proteins, J. S. Davies (reviewed by Douglas Young); Virtual Screening for Bioactive Molecules, H.-J. Böhm and G. Schneider (reviewed by Dr John B. O. Mitchell); Biologically Active Natural Products: Pharmaceuticals, S. J. Cutler and H. G. Cutler (reviewed by John Mann)." Natural Product Reports 18, no. 3 (2001): 356–60. http://dx.doi.org/10.1039/b103593m.

Texte intégral

Styles APA, Harvard, Vancouver, ISO, etc.

Nous offrons des réductions sur tous les plans premium pour les auteurs dont les œuvres sont incluses dans des sélections littéraires thématiques. Contactez-nous pour obtenir un code promo unique!