Thèses : « Medical laboratory science »

1

Kovach, Alison A. « Challenges of Medical Laboratory Science and Medical Laboratory Technology Program Directors ». Youngstown State University / OhioLINK, 2015. http://rave.ohiolink.edu/etdc/view?acc_num=ysu1433424508.

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Price, Travis Miles. « Emotional Intelligence in Medical Laboratory Science ». DigitalCommons@USU, 2013. https://digitalcommons.usu.edu/etd/1711.

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The purpose of this study was to explore the role of emotional intelligence (EI) in medical laboratory science, as perceived by laboratory administrators. To collect and evaluate these perceptions, a survey was developed and distributed to over 1,400 medical laboratory administrators throughout the U.S. during January and February of 2013. In addition to demographic-based questions, the survey contained a list of 16 items, three skills traditionally considered important for successful work in the medical laboratory as well as 13 EI-related items. Laboratory administrators were asked to rate each item for its importance for job performance, their satisfaction with the item's demonstration among currently working medical laboratory scientists (MLS) and the amount of responsibility college-based medical laboratory science programs should assume for the development of each skill or attribute. Participants were also asked about EI training in their laboratories and were given the opportunity to express any thoughts or opinions about EI as it related to medical laboratory science. This study revealed that each EI item, as well as each of the three other items, was considered to be very or extremely important for successful job performance. Administrators conveyed that they were satisfied overall, but indicated room for improvement in all areas, especially those related to EI. Those surveyed emphasized that medical laboratory science programs should continue to carry the bulk of the responsibility for the development of technical skills and theoretical knowledge and expressed support for increased attention to EI concepts at the individual, laboratory, and program levels.

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Cheng, Leung Wai-lin Winnie. « An evaluation of a laboratory report writing unit for medical laboratory science students ». Click to view the E-thesis via HKUTO, 1989. http://sunzi.lib.hku.hk/HKUTO/record/B38626500.

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Cheng, Winnie, et 鄭梁慧蓮. « An evaluation of a laboratory report writing unit for medical laboratory science students ». Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 1989. http://hub.hku.hk/bib/B38626500.

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Kirby, Beverly A. « The future of clinical laboratory science a Delphi study / ». Morgantown, W. Va. : [West Virginia University Libraries], 2007. https://eidr.wvu.edu/etd/documentdata.eTD?documentid=5424.

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Thesis (Ed. D.)--West Virginia University, 2007.
Title from document title page. Document formatted into pages; contains xi, 418 p. Includes abstract. Includes bibliographical references (p. 146-158).

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Madan, Nilia M. « Predictors of Success : Medical Laboratory Associate in Science Degree Program ». FIU Digital Commons, 2012. http://digitalcommons.fiu.edu/etd/672.

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Hospitals and healthcare facilities in the United States are facing serious shortages of medical laboratory personnel, which, if not addressed, stand to negatively impact patient care. The problem is compounded by a reduction in the numbers of academic programs and resulting decrease in the number of graduates to keep up with the increase in industry demands. Given these challenges, the purpose of this study was to identify predictors of success for students in a selected 2-year Medical Laboratory Technology Associate in Science Degree Program. This study examined five academic factors (College Placement Test Math and Reading scores, Cumulative GPA, Science GPA, and Professional [first semester laboratory courses] GPA) and, demographic data to see if any of these factors could predict program completion. The researcher examined academic records for a 10-year period (N =158). Using a retrospective model, the correlational analysis between the variables and completion revealed a significant relationship (p < .05) for CGPA, SGPA, CPT Math, and PGPA indicating that students with higher CGPA, SGPA, CPT Math, and PGPA were more likely to complete their degree in 2 years. Binary logistic regression analysis with the same academic variables revealed PGPA was the best predictor of program completion (p < .001). Additionally, the findings in this study are consistent with the academic part of the Bean and Metzner Conceptual Model of Nontraditional Student Attrition which points to academic outcome variables such as GPA as affecting attrition. Thus, the findings in this study are important to students and educators in the field of Medical Laboratory Technology since PGPA is a predictor that can be used to provide early in-program intervention to the at-risk student, thus increasing the chances of successful timely completion.

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Lindor, Anna, et Evelina Pettersson. « The prevalence of hypertension in young medical students in Vietnam ». Thesis, Hälsohögskolan, Högskolan i Jönköping, HHJ. Biomedicinsk plattform, 2018. http://urn.kb.se/resolve?urn=urn:nbn:se:hj:diva-40485.

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Juma, Mahmoud. « Analys av leukocytinnehållet i framställd plasmakomponent för transfusion ». Thesis, Örebro universitet, Hälsoakademin, 2011. http://urn.kb.se/resolve?urn=urn:nbn:se:oru:diva-15576.

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Katagiri, Fumihiko. « Studies on clinical pharmaceutical science and laboratory medical application using plasma physiological active peptides levels as biomarkers ». 京都大学 (Kyoto University), 2008. http://hdl.handle.net/2433/137183.

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Kraj, Barbara. « Incorporation of Molecular Diagnostics into Medical Laboratory Science Curriculum : Clinical Facilities Expectations. An Asynchronous, Iterative, Online Delphi Study ». VCU Scholars Compass, 2015. http://scholarscompass.vcu.edu/etd/3721.

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The medical laboratory science (MLS) profession is in need for published molecular diagnostics competency-based standards and curriculum. To assess their expectations of new MLS graduates, professionals performing and supervising performance of clinical molecular assays were surveyed to rate the importance of relevant cognitive and psychomotor learning objectives. A modified, asynchronous, iterative online Delphi process was utilized for assessment of consensus on the importance of the objectives. The survey was delivered through online REDCap application. Program directors of 221 MLS programs accredited by the National Accrediting Agency for Clinical Laboratory Science (NAACLS) were asked to forward the first Delphi survey to target participants at their affiliated clinical sites. Ninety-four experts submitted complete surveys, including 88 who provided email addresses, indicating agreement to participate in future Delphi rounds. Most of the participants were certified by ASCP or NCA (81.9%), had over 10 years of laboratory experience (76.6%), and worked in a hospital setting (43.6%). The reliability of the surveys, assessed using Cronbach’s alpha, was 0.96 and 0.97. In the second survey, the objectives assigned low importance by the majority were removed; and others, assigned high importance were expanded. Respondents were given the opportunity to confirm or change their opinion on the objectives after reviewing quantitative results and narrative comments collected in the preceding survey. Upon completion of the Delphi process, 25 essential items were identified as necessary for inclusion in the entry-level MLS curriculum. These concepts and objectives focused on basic molecular biology principles and general molecular laboratory operations, including practical knowledge of techniques designed to maintain specimen integrity and intense theoretical background of the polymerase chain reaction, as well as comprehension of the principles of laboratory assays designed for pathogens most commonly tested for using molecular methods. In this study, the investigator also provided information on the preferred number of contact hours devoted to each group of the identified essential items. The goal of creating the list of essential concepts and objectives was to share it with MLS educators, the NAACLS and the provider of MLS certification exam, the American Society for Clinical Pathology Board of Certification (ASCP-BOC), to contribute to the existing exam content guidelines.

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Latorre, Malcolm. « The Physical Axon : Modeling, Simulation and Electrode Evaluation ». Doctoral thesis, Linköpings universitet, Avdelningen för medicinsk teknik, 2017. http://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-138587.

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Electrodes are used in medicine for detection of biological signals and for stimulating tissue, e.g. in deep brain stimulation (DBS). For both applications, an understanding of the functioning of the electrode, and its interface and interaction with the target tissue involved is necessary. To date, there is no standardized method for medical electrode evaluation that allows transferability of acquired data. In this thesis, a physical axon (Paxon) potential generator was developed as a device to facilitate standardized comparisons of different electrodes. The Paxon generates repeatable, tuneable and physiological-like action potentials from a peripheral nerve. It consists of a testbed comprising 40 software controlled 20 μm gold wires embedded in resin, each wire mimicking a node of Ranvier. ECG surface Ag-AgCl electrodes were systematically tested with the Paxon. The results showed small variations in orientation (rotation) and position (relative to axon position) which directly impact the acquired signal. Other electrode types including DBS electrodes can also be evaluated with the Paxon. A theoretical comparison of a single cable neuronal model with an alternative established double cable neuron model was completed. The output with regards to DBS was implemented to comparing the models. These models were configured to investigate electrode stimulation activity, and in turn to assess the activation distance by DBS for changes in axon diameter (1.5-10 μm), pulse shape (rectangular biphasic and rectangular, triangular and sinus monophasic) and drive strength (1-5 V or mA). As both models present similar activation distances, sensitivity to input shape and computational time, the neuron model selection for DBS could be based on model complexity and axon diameter flexibility. An application of the in-house neuron model for multiple DBS lead designs, in a patient-specific simulation study, was completed. Assessments based on the electric field along multiple sample planes of axons support previous findings that a fixed electric field isolevel is sufficient for assessments of tissue activation distances for a predefined axon diameter and pulse width in DBS.
Elektroder används inom sjukvården, både för att mäta biologiska signaler, t.ex. hjärtats aktivitet med EKG, eller för att stimulera vävnad, t.ex. vid djup hjärnstimulering (DBS). För båda användningsområdena är det viktigt med en grundläggande förståelse av elektrodens interaktion med vävnaden. Det finns ingen standardiserad metod för att utvärdera medicinsk elektroders dataöverföringsfunktion. I den här avhandlingen presenteras en metod för att underlätta elektrodtestning. En hårdvarumodell av ett axon (Paxon) har utvecklats. Paxon kan programmeras för att efterlikna repeterbara aktionspotentialer från en perifer nerv. Längs axonet finns 40 noder, vilka var och en består av en tunn (20 μm) guldtråd inbäddad i harts och därefter kopplad till elektronik. Denna testbädd har använts för att undersöka EKG elektroders egenskaper. EKG elektroderna visade på variationer i orientering och position i relation till Paxon. Detta har en direkt inverkan på den registrerade signalen. Även andra elektrotyper kan testas i Paxon, t.ex. DBS elektroder. En teoretisk jämförelse mellan två neuronmodeller med olika komplexitet, anpassade för användning vid DBS studier, har utförts. Modellerna konfigurerades för att studera inverkan på aktiveringsavstånd från olika axondiametrar, stimulationspuls och stimulationsstyrka. Då båda modellerna visade likvärdiga aktiveringsavstånd och beräkningstid så förordas den enklare neuronmodellen för DBS simuleringar. En enklare modell kan lättare introduceras i klinisk verksamhet. Simuleringarna stöder tidigare resultat som visat att det elektriska fältet är en bra parameter för presentation av resultat vid simulering av DBS. Metoden exemplifieras vid simulering av aktiveringsavstånd och elektriska fältets utbredning för olika typer av DBS elektroder i en patient-specifik studie.

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Levin, Nadine S. « Enacting molecular complexity : data and health in the metabonomics laboratory ». Thesis, University of Oxford, 2013. http://ora.ox.ac.uk/objects/uuid:3a439abb-e010-404a-af90-ffa547acc59f.

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In this dissertation, I examine how biological data practices enable researchers to interact with and enact biological life in statistical ways, and how this poses challenges to the use and integration of biological knowledge with clinical practices. Instead of considering data as a pre-existing cognitive representation of the world, I combine scholarship on the anthropology of science with scholarship from science and technology studies to consider data as a form of material practice. I consider, in other words, how data is intertwined with technologies, people, and values, such that data is used to make normative and naturalized claims about biology and disease. To explore the generation, interpretation, and use of biological data, I focus on the field of “metabonomics”—the post-genomic study of metabolism—as it is carried out within the Biomolecular Medicine Laboratory (BMM) at Imperial College London. In doing so, I examine how metabonomics researchers use biochemical techniques and multivariate statistics to investigate metabolism and disease. After providing an overview of the literature, central questions, and methodology that frame this dissertation, I examine how multivariate statistical practices are central to the historical identity and epistemic culture of metabonomics research at the BMM. From there, I demonstrate how multivariate statistics require and enable metabonomics to enact metabolism as an inherently complex entity. Consequently, I examine how researchers struggle to assign the categories of “normal” and “abnormal” to dynamic notions of metabolism and health. I then explore how the translation of metabonomics knowledge into clinical practices places value on multivariate forms and large volumes of information, eclipsing the importance of human interpretation and judgment. Finally, I examine how metabonomics research is used to develop personalized medicine, but in ways that make it difficult to address the health of individual patients.

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Yang, Jing. « Determination of peramivir and its toxicokinetics in beagle dogs ». Thesis, University of Macau, 2011. http://umaclib3.umac.mo/record=b2524966.

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Zurgani, Emad K. A. « Documentation of the body transformations during the decomposition process : from the crime scene to the laboratory ». Thesis, University of Huddersfield, 2018. http://eprints.hud.ac.uk/id/eprint/34690/.

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Forensic science is defined as the application of scientific or technical practices to the recognition, collection, analysis, and interpretation of evidence for criminal and civil law or regulatory issues. A combination of computer science in the field of 3D reconstruction and molecular biology science and techniques were employed in this research aims to document and record a complete picture of the body decomposition process including the changes of the microbiome over the decomposition process. In this thesis, the possibility to reconstruct the crime scene and the decomposition process was investigated. In addition, a 3D model aiming to integrate the biological and thanatological information was generated. The possibility of utilising Autodesk 123D Catch software as a new tool for 3D reconstruction of a crime scene was thoroughly evaluated. First experiments demonstrated that the number of photos required to obtain the best result was specified to be from 20 to 30 photos as a minimum. In addition, significant experiments were performed in different conditions of sizes, locations, and different involved materials. The measurements were obtained from the models using the same software were compared with the real measurements of the tested objects. The result of the correlation between real and estimated measurements showed a very strong agreement ranging from 0.994 to 1.000. With reference to the documentation of the decomposition process, there are different factors, intrinsic and extrinsic, have been reported affecting the decomposition of a carrion/body. These factors mainly interact with the rates of the biological and chemical reaction happening after death. The biological reactions are mainly due to the activity of microorganism and insects. Pigs (Sus scrofa domesticus) were used as a model for human studies and the results obtained have been applied to other mammals without considering the effect of fur on the decomposition process and on the insect and microbial colonisation. In order to investigate this point, rabbits (Oryctolagus cuniculus) with and without fur were used in two sets of experiments at Huddersfield in summer 2014 and in spring 2015. The results obtained in this study showed a similarity of the decomposition stages between animals with and without fur. However, the decomposition process was faster during the summer due to the fast of insect colonisation and activity. In addition, the entomological data collected during the summer and spring experiments were demonstrated that the same taxa nearly were present in both seasons, except Hydrotaea (Diptera, Muscidae), which was presented only in the summer experiment, moreover, only one sample of Lucilia sericata (Calliphoridae) was detected in the spring season. Differences in colonisation time were observed only in spring experiment; animals without fur were colonised two days before animals with fur. The season could have affected the insect’s activity and the spread of the decomposition volatiles. The microbial communities during the decomposition process were investigated using BIOLOG EcoPlateTM and the hypervariable V1-3 region of 16S rRNA gene was used for their molecular identification based on pyrosequencing. Eurofins Genomic Operon using 454-GS Junior pyrosequencing platform (Roche) carried out these analyses. The functional diversity of the bacterial communities on all carcasses samples showed a considerable variability depending on the stage of the decomposition and the sampling region (Oral cavity, skin and interface-sand-carrion) in both seasons. Furthermore, over the molecular analyses of bacterial communities at the phylum level, four main phyla of bacteria were detected among analysed carrion during the decomposition process. These phyla were changed significantly during the stages of the decomposition and between sampling regions. While no difference was observed due to presence or absence of fur. On the other hand, the analysis at the family level was able to highlight differences at the temporal scale but as well as carrion with and without fur. The statistical analysis results showed a significant difference in the bacterial community family distribution among the presence of fur and among the decomposition stages, with significant differences among sampling regions and seasons.

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D'Souza, Aswin Cletus. « Automated counting of cell bodies using Nissl stained cross-sectional images ». [College Station, Tex. : Texas A&M University, 2007. http://hdl.handle.net/1969.1/ETD-TAMU-2035.

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Rahimi, Bahol. « Implementation of Health Information Systems ». Licentiate thesis, Linköping University, Linköping University, MDA - Human Computer Interfaces, 2008. http://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-15677.

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Healthcare organizations now consider increased efficiency, reduced costs, improved patient care and quality of services, and safety when they are planning to implement new information and communication technology (ICT) based applications. However, in spite of enormous investment in health information systems (HIS), no convincing evidence of the overall benefits of HISs yet exists. The publishing of studies that capture the effects of the implementation and use of ICT-based applications in healthcare may contribute to the emergence of an evidence-based health informatics which can be used as a platform for decisions made by policy makers, executives, and clinicians. Health informatics needs further studies identifying the factors affecting successful HIS implementation and capturing the effects of HIS implementation. The purpose of the work presented in this thesis is to increase the available knowledge about the impact of the implementation and use of HISs in healthcare organizations. All the studies included in this thesis used qualitative research methods. A case study design and literature review were performed to collect data.

This thesis’s results highlight an increasing need to share knowledge, find methods to evaluate the impact of investments, and formulate indicators for success. It makes suggestions for developing or extending evaluation methods that can be applied to this area with a multi-actor perspective in order to understand the effects, consequences, and prerequisites that have to be achieved for the successful implementation and use of IT in healthcare. The results also propose that HIS, particularly integrated computer-based patient records (ICPR), be introduced to fulfill a high number of organizational, individualbased, and socio-technical goals at different levels. It is therefore necessary to link the goals that HIS systems are to fulfill in relation to short-term, middle-term, and long-term strategic goals. Another suggestion is that implementers and vendors should direct more attention to what has been published in the area to avoid future failures.

This thesis’s findings outline an updated structure for implementation planning. When implementing HISs in hospital and primary-care environments, this thesis suggests that such strategic actions as management involvement and resource allocation, such tactical action as integrating HIS with healthcare workflow, and such operational actions as user involvement, establishing compatibility between software and hardware, and education and training should be taken into consideration.

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Bjällmark, Anna. « New ultrasonographic approaches to monitoring cardiac and vascular function ». Doctoral thesis, KTH, Medicinsk teknik, 2009. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-11760.

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Atherosclerotic cardiovascular disease is the leading cause of death worldwide. To decrease mortality and morbidity in cardiovascular disease, the development of accurate, non-invasive methods for early diagnosis of atherosclerotic cardiac and vascular engagement is of considerable clinical interest. Cardiovascular ultrasound imaging is today the cornerstone in the routine evaluation of cardiovascular function and recent development has resulted in two new techniques, tissue velocity imaging (TVI) and speckle tracking, which allow objective quantification of cardiovascular function. TVI and speckle tracking are the basis for three new approaches to cardiac and vascular monitoring presented in this thesis: wave intensity wall analysis (WIWA), two-dimensional strain imaging in the common carotid artery, and the state diagram of the heart. WIWA uses longitudinal and radial strain rate as input for calculations of wave intensity in the arterial wall. In this thesis, WIWA was validated against a commercially available wave intensity system, showing that speckle tracking-derived strain variables can be useful in wave intensity analysis. WIWA was further tested in patients with end stage renal disease and documented high mortality in cardiovascular disease. The latter study evaluated the effects of a single session of hemodialysis using WIWA and TVI variables and showed improved systolic function after hemodialysis. The results also indicated that preload-adjusted early systolic wave intensity obtained by the WIWA system may contribute in the assessment of left ventricular contractility in this patient category. Two-dimensional strain imaging in the common carotid artery is a new approach showing great potential to detect age-dependent differences in mechanical properties of the common carotid artery. Among the measured strain variables, global circumferential strain had the best discriminating performance and appeared to be superior to conventional measures of arterial stiffness such as elastic modulus and β stiffness index. The state diagram is a visualisation tool that provides a quantitative overview of the temporal interrelationship of mechanical events in the left and right ventricles. Case examples and a small clinical study showed that state diagrams clearly visualize cardiac function and can be useful in the detection of non ST-elevation myocardial infarction (NSTEMI). Even though WIWA, two-dimensional strain imaging in the common carotid artery and the state diagram show potential to be useful in the evaluation of cardiovascular function, there still remains a considerable amount of work to be done before they can be used in the daily clinical practice.
QC 20100705

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Boltshauser, Rasmus. « Development of a Novel Device for Optimal Sample Blood Volume Collection from Patients with Sepsis ». Thesis, KTH, Medicinteknik och hälsosystem, 2020. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-279133.

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When performing sepsis diagnosis, the most important preanalytical variable is blood volume. Too little blood increases the risk for false negatives whereas overfilling causes increased risk for false positives. Even though this fact is known, there are case studies showing that in a majority of tests, the taken blood sample volume is not the recommended amount. As previously tried methods have been limited in their ability to tackle the problem this study aimed at creating a technical device to aid healthcare providers with blood volume sample collection. As a base, the double diamond approach by the Design Council was used. This design approach splits up the design process in four distinctly different phases (discover, define, develop, and deliver) all using their own methods to aid the creative process. After completing the discover and define phase it was determined that a non-contact capacitance liquid level sensor could operate as an ideal blood volume sample device. During the development and delivery phase prototypes were created and evaluated. The final results of this work could not give conclusive evidence concerning if a non-contact liquid level sensor could operate as an ideal blood volume collection device. The methodological approach used in this thesis can be used as inspiration for a designer to create a device for a similar or different purpose. Moreover, information from this thesis can also work as reference material to develop a device to perform ideal blood volume sample collection. Such a device would have the potential to be an essential part of the everyday workflow in sample collection from patients with sepsis worldwide and would aid in ensures effective and fast diagnostics.

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Forsgren, Mikael. « The Non-Invasive Liver Biopsy : Determining Hepatic Function in Diffuse and Focal LiverDisease ». Doctoral thesis, Linköpings universitet, Avdelningen för radiologiska vetenskaper, 2017. http://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-136545.

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The liver is one of the largest organs within the human body and it handles many vital tasks such as nutrient processing, toxin removal, and synthesis of important proteins. The number of people suffering from chronic liver disease is on the rise, likely due to the present ‘western’ lifestyle. As disease develops in the liver there are pathophysiological manifestations within the liver parenchyma that are both common and important to monitor. These manifestations include inflammation, fatty infiltration (steatosis), excessive scar tissue formation (fibrosis and cirrhosis), and iron loading. Importantly, as the disease progresses there is concurrent loss of liver function. Furthermore, postoperative liver function insufficiency is an important concern when planning surgical treatment of the liver, because it is associated with both morbidity and mortality. Liver function can also be hampered due to drug-induced injuries, an important aspect to consider in drug-development. Currently, an invasive liver needle biopsy is required to determine the aetiology and to stage or grade the pathophysiological manifestations. There are important limitations with the biopsy, which include, risk of serious complications, mortality, morbidity, inter- and intra-observer variability, sampling error, and sampling variability. Cleary, it would be beneficial to be able investigate the pathophysiological manifestations accurately, non-invasively, and on regional level. Current available laboratory liver function blood panels are typically insufficient and often only indicate damage at a late stage. Thus, it would be beneficial to have access to biomarkers that are both sensitive and responds to early changes in liver function in both clinical settings and for the pharmaceutical industry and regulatory agencies. The main aim of this thesis was to develop and evaluate methods that can be used for a ‘non-invasive liver biopsy’ using magnetic resonance (MR). We also aimed to develop sensitive methods for measure liver function based on gadoxetate-enhanced MR imaging (MRI). The presented work is primarily based on a prospective study on c. 100 patients suffering from chronic liver disease of varying aetiologies recruited due to elevated liver enzyme levels, without clear signs of decompensated cirrhosis. Our results show that the commonly used liver fat cut-off for diagnosing steatosis should be lowered from 5% to 3% when using MR proton-density fat fraction (PDFF). We also show that MR elastography (MRE) is superior in staging fibrosis. Finally we presented a framework for quantifying liver function based on gadoxetate-enhanced MRI. The method is based on clinical images and a clinical approved contrast agent (gadoxetate). The framework consists of; state-of the-art image reconstruction and correction methods, a mathematical model, and a precise model parametrization method. The model was developed and validated on healthy subjects. Thereafter the model was found applicable on the chronic liver disease cohort as well as validated using gadoxetate levels in biopsy samples and blood samples. The liver function parameters correlated with clinical markers for liver function and liver fibrosis (used as a surrogate marker for liver function). In summary, it should be possible to perform a non-invasive liver biopsy using: MRI-PDFF for liver fat and iron loading, MRE for liver fibrosis and possibly also inflammation, and measure liver function using the presented framework for analysing gadoxetate-enhanced MRI. With the exception of an MREtransducer no additional hardware is required on the MR scanner. The liver function method is likely to be useful both in a clinical setting and in pharmaceutical trials.

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Andersson, Frida. « Dementia ; common cause of suicide among elderly ? » Thesis, Uppsala University, Department of Medical Biochemistry and Microbiology, 2006. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-7164.

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Elderly committing suicide can be in a “preclinical phase” of dementia. Depressive symptoms may indicate a risk to develop a disease of dementia, for example Alzheimer’s Disease. Today almost 10% of the Swedish population older than 65 years suffer from a cognitive impairment diagnosed as dementia. Symptoms of dementia are associated with degenerative changes in the brain caused by a deposition of amyloid, leading among others things to a nerve cell death. A clinical diagnosis can be hard to set, and a definitive diagnose can only be set after a pathological examination, which only is possible after death. For this study we used Congo red staining of brains sections to find amyloid in autopsies from elderly people committing suicide. 35 cases (>60 year) were studied. Of the 35 cases 1/3 showed to be positive for amyloid deposition. This result in addition to other studies suggest that depressive symptoms is a “preclinical phase” of dementia, and therefore the suicide risk for this group must be consider to be elevated. However, more reliable prospective studies most be done to confirm this retrospective study.

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Lindblad, Erik. « Designing a framework for simulating radiology information systems ». Thesis, Linköping University, Department of Computer and Information Science, 2008. http://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-15211.

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In this thesis, a very flexible framework for simulating RIS is designed to beused for Infobroker testing. Infobroker is an application developed by MawellSvenska AB that connects RIS and PACS to achieve interoperability by enablingimage and journal data transmission between radiology sites. To put the project in context, the field of medical informatics, RIS and PACS systems and common protocols and standards are explored. A proof-of-concept implementation of the proposed design shows its potential and verifies that it works. The thesis concludes that a more specialized approach is preferred.

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Lundström, Jonathan, et Joel Skagersten. « Optimering samt implementering av Harts automatiserade färgningsmetod : Ersättning av Verhoeffs manuella elastinfärgning ». Thesis, Jönköping University, HHJ, Avd. för naturvetenskap och biomedicin, 2021. http://urn.kb.se/resolve?urn=urn:nbn:se:hj:diva-52932.

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Elastinfibrer ger blodkärl och andra vävnader deras flexibilitet. Elastinfärgning är relevant när man misstänker melanom, temporalis artrit, venös invasion och efter blodkärlsoperationer. Syftet var att med hjälp av olika vävnadstyper optimera och implementera den automatiserad elastinfärgningen enligt Hart, för att ersätta den nuvarande manuella elastinfärgning enligt Verhoeff vid patologilaboratoriet på länssjukhuset Ryhov, Jönköping. Colon, njure, hud samt navelsträng färgades med den automatiska metoden enligt Hart för att hitta optimala inställningar. Vävnader från samma områden färgades med den manuella metoden enligt Verhoeff samt den automatiska metoden enligt Hart för att jämföra dem. Snitten bedömdes av en läkare så allt färgades som det skulle. Optimeringen av Harts metod resulterade i en inkubationstid på tolv minuter samt en optimal färgningsbehandling utan xylen. Resultaten av jämförelsen mellan den automatiska metoden enligt Hart och den manuella metoden enligt Verhoeff visar att den automatiska metoden enligt Hart ger bättre kontrast samt bakgrundsinfärgning. Slutsatsen blev att den automatiska metoden enligt Harts var bättre än den manuella metoden enligt Verhoeff, att i framtida studier studera möjligheten att byta ut läskningen mot till exempel ytterligare ett etanoldop samt att byta ut snitten av navelsträngen mot snitt av lever.
Elastic fibres ensure blood vessels and other tissues flexibility. Elastic staining of tissue is relevant when there is suspicion of melanoma, temporalis arteritis, venous invasion and after operations on blood vessels. The aim of the study was with the help of different tissue samples optimize and implement Hart´s elastic staining method as a substitute for Verhoeff’s at pathology lab at county hospital Ryhov, Jönköping. Colon, kidney, skin, and umbilical cord cross section got stained with Hart´s automated elastic staining method to evaluate the optimal staining procedure. Same region of the tissues was stained with Verhoeff´s manual elastic staining method and Hart´s method. All cross section were assessed and compared with the help of a pathologist doctor. Optimization of Hart´s method resulted in an incubation period of twelve minutes and optimal staining procedure without xylene. Result of comparison between Hart´s staining method and Verhoeff´s staining method showed that Hart´s staining method had a better contrast and background. Conclusions of the study was that Hart´s staining method was better than Verhoeff´s staining method, further studies could include research about a substitution of the blotting step with an extra ethanol bath as an example and liver tissue instead of the umbilical cord.

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Larsson, Patrik. « Inhibiting the IGF-1 receptor with the cyclolignan Picropodophyllin : an in vitro study of ovulation, implantation and receptivity in a mouse model ». Thesis, Uppsala University, Department of Medical Biochemistry and Microbiology, 2008. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-89109.

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Picropodophyllin (PPP) is an analogue of the anti tumour lignan podophyllotoxin with the unique ability to selectively inhibit the receptor of Insulin like growth factor 1(IGF-1). IGF-1 is believed to play an important part in development of the endometrium facing implantation. With PPP treated mice, studies can be made to measure gene expression from tissue of both treated and untreated mice to compare the role of IGF-1 regarding ovulation, implantation and receptivity. The aim of this study was to analyze gene expression of some steroid hormone receptors and cytokines in ovaries from mice treated with PPP. In this study, seven mice were treated with PPP at different times and tissue was collected. PCR-primers for cDNA sequences of estrogene receptor α, estrogene receptor β, progesterone receptor A, progesterone receptor B, growth hormone receptor, interleukin 1 α, interleukin 1 β, tumour necrosis factor α and androgen receptor were used. Real Time PCR was run with the samples and gene expression was measured. The results of this study showed that the inhibition of IGF-1 receptor interacted with IGF-1 which lead to altered levels of estrogene receptor alpha, progesterone receptor, growth hormone receptor and androgen receptor that can decrease ovulation. The results also showed the differences in gene products between treated and untreated samples, suggesting that IGF-1 plays an important role regarding ovulation.

Studier med hjälp av den selektiva insulinlika tillväxtfaktor 1 receptorn (IGF-1R) antagonisten; picropodof?phyllin (PPP), hur samspelet mellan livmoderslemhinnan och implantationsprocessen, samt hur ovulationen påverkas av insulinlika tillväxtfaktorn 1 (IGF-1) kan nu utföras. IGF-1 tros ha en viktig roll för den reproduktiva processen, där den påverkar ovulation, implantation och embryoutveckling. IGF-familjen består av tre ligander; insulin, IGF-1 och IGF-2. IGF transporteras bundet till bindarprotein (IGFBP). Medlemmarna i IGF receptorfamiljen kan binda IGF-1, IGF-2 och insulin fast med olika affinitet. PPP som är en cykloligan, är en analog från podofyllotoxin och fungerar som en syntetisk IGF-1 receptorantagonist, som selektivt inhiberar receptorns aktivitet. PPP tros även kunna nedreglera genexpression av receptorn. Tre tidigare projektarbeten har utförts på vävnader från möss injicerade med PPP. Tyngdpunkterna i dessa arbeten har legat på immunhistokemiska studier av IGF-1 i reproduktionsorgan från möss, uttryck av IGF-1, dess receptor och bindarprotein 1 i ovarier och uterus efter behandling med PPP. I denna studie användes vävnad samt cDNA från sju möss behandlade med PPP, i olika stadier av reproduktionen samt även icke behandlade möss. Studiens syfte var att med sanntids-PCR jämföra genuttryck från östrogenreceptor α och β, progesteronreceptor A och B, tillväxthormonreceptor, Interleukin 1 α och β, ’tumor necrosis’ faktor α samt androgenreceptor i vävnad från PPP-behandlade och obehandlade möss och genom de erhållna resultaten från ovarievävnaden utläsa effekten på ovulationen och från uterusvävnaden effekten på implantation och receptivitet. Studieresultaten visade att IGF-1s frånvaro gav förändrade nivåer av genprodukter, som medförde minskad ovulationen. Studien visade att IGF-1s roll vid ovulationen var väsentlig.

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Meerits, Jonna. « Blodtrycksmanschett eller manuell handkompression vid bedömning av venös insufficiens i Vena saphena magna ». Thesis, Örebro universitet, Hälsoakademin, 2011. http://urn.kb.se/resolve?urn=urn:nbn:se:oru:diva-15784.

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Bakgrund: Venduplex har på senare år vuxit fram som den mest använda metoden vid diagnostik av venös insufficiens eftersom det är en ofarlig, kostnadseffektiv, non-invasiv metod med hög sensitivitet och specificitet där både den anatomiska placeringen av insufficiensen samt den funktionella förändringen av venen kan påvisas direkt vid undersökningen. Olika manövrar kan genomföras för att påfresta venklaffarna och därmed framkalla en eventuell reflux, men det är oklart om de olika manövrerna kan påverka den reflux som provoceras fram och därmed också påverkar bedömningen av insufficiensgraden. Syfte: Syftet med studien var att undersöka om det förelåg skillnad i graderingen av insufficiensen samt om den maximala refluxhastigheten påverkades beroende på vilken manöver som användes för att framkalla en reflux. Metod: 20 v. saphena magna mitt på låret med påvisad reflux deltog i studien. Två upprepade mätningar genomfördes där venklaffarna provocerades med hjälp av en blodtrycksmanschett över vaden, med en manuell kompression över låret samt med en manuell kompression över vaden. Resultat: Den maximala refluxhastigheten blev 0,33±0,20m/s när en blodtrycksmanschett över vaden användes, 0,31±0,22m/s när en manuell handkompression över vaden användes samt 0,17±0,16m/s när en manuell lårkompression genomfördes. Slutsats: Ingen signifikant skillnad på maximal refluxhastighet kunde konstateras när en automatisk uppblåsbar blodtrycksmanschett över vaden jämfördes med en manuell handkompression över vaden. En signifikant högre maximal refluxhastighet konstaterades när en manuell vadkompression jämfördes med en manuell lårkompression.

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Sjölund, Fanny. « Förhållandet mellan hudblodflöde och fysisk aktivitet ». Thesis, Örebro universitet, Hälsoakademin, 2011. http://urn.kb.se/resolve?urn=urn:nbn:se:oru:diva-15834.

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SAMMAMFATTNING Bakgrund: Reaktiv hyperemi definieras som ett övergående ökat blodflöde över det normala efter en tids ischemi. Det kan registreras med laserdopplerteknik. Att registrera reaktiv hyperemi är ett sätt att värdera mikrocirkulationen. Det finns många flödesvariabler att studera varav tid till maxflöde efter ocklusion är en. Det har gjorts studier som undersöker om det finns ett samband mellan reaktiv hyperemi och fysisk aktivitet/syreupptagningsförmåga. Det har inte gjorts någon studie som undersöker tid till maxflöde och fysisk aktivitet. Syftet var att undersöka om det finns ett samband mellan fysisk aktivitet och reaktiv hyperemi med avseende på tid till maxflöde. Material och metod: Testpersoner fick bära en accelerometer en vecka under dygnets alla vakna timmar samt göra en registrering av reaktiv hyperemi med laserdoppler. För statistiska beräkningar användes oparat T-test för att undersöka skillnad mellan olika grad av fysisk aktivitet och tid till maxflöde. Resultat: Ingen statistiskt signifikant skillnad mellan olika aktivitetsgrad och tid till maxflöde kunde observeras. Slutsats: Den här studien visade inte på statistiskt signifikant samband mellan blodflöde och fysisk aktivitet.

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Zajac, Jakub. « Assessment of Ventricular Function in Normal and Failing Hearts Using 4D Flow CMR ». Doctoral thesis, Linköpings universitet, Avdelningen för kardiovaskulär medicin, 2017. http://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-141006.

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Heart failure is a common disorder and a major cause of illness and death in the population, creating an enormous health-care burden. It is a complex condition, representing the end-point of many cardiovascular diseases. In general heart failure progresses slowly over time and once it is diagnosed it has a poor prognosis which is comparable with that of many types of cancer. The heart has an ability to adapt in response to long lasting increases in hemodynamic demand; the heart conforms its shape and size in order to maintain adequate cardiac output. This process is called remodeling and can be triggered by pathologies such as hypertension or valvular disease. When the myocardial remodeling is maintained chronically it becomes maladaptive and is associated with an increased risk of heart failure. In many cases, heart failure is associated with left bundle branch block (LBBB). This electrical disturbance leads to dyssynchronous left ventricular (LV) contraction and relaxation which may contribute to cardiac dysfunction and ultimately heart failure. Mechanical dyssynchrony can be treated with cardiac resynchronization therapy (CRT). However, many heart failure patients do not demonstrate clinical improvement despite CRT. Blood flow plays an important role in the normal development of the fetal heart. However, flow-induced forces may also induce changes in the heart cells that could lead to pathological remodeling in the adult heart. Until recently, measurement tools have been inadequate in describing the complex three-dimensional and time-varying characteristics of blood flow within the beating heart. 4D (3D + time) flow cardiovascular magnetic resonance (CMR) enables acquisition of three-dimensional, three-directional, time-resolved velocity data from which visualization and quantification of the blood flow patterns over a complete cardiac cycle can be performed. In this thesis, novel 4D Flow CMR based methods are used to study the intraventricular blood flow in healthy subjects and heart failure patients with and without ventricular dyssynchrony in order to gain new knowledge of the ventricular function. Different flow components were assessed in normal heart ventricles. It was found that inflowing blood that passes directly to outflow during the same heartbeat (the Direct Flow component) was larger and possessed more kinetic energy (KE) than other flow components. Diastolic flow through the normal heart appears to create favorable conditions for effective systolic ejection. This organized blood flow pattern within the normal LV is altered in heart failure patients and is associated with decreased preservation of KE which might be unfavorable for efficient LV ejection. Inefficient flow of blood through the heart may influence diastolic wall stress, and thus contribute to pathological myocardial remodeling. In dyssynchronous LVs of heart failure patients with LBBB, Direct Flow showed even more reduced preservation of KE compared to similarly remodeled LVs without LBBB. Furthermore, in LBBB patients, LV filling hemodynamic forces, acting on the myocardium, were more orthogonal to the main flow direction compared to patients without LBBB. Deviation of LV flow forces and reduction of KE preservation and may reflect impairment of LV diastolic function and less efficient ensuing ejection related to dyssynchrony in these failing ventricles. Blood flow patterns were also studied with respect to fluctuations of the velocity of the flow (turbulent flow) in normal and failing LVs. In failing hearts, turbulent kinetic energy (TKE) was higher during diastole than in healthy subjects. TKE is a cause of energy loss and can thus be seen as a measure of flow inefficiency. Elucidating the transit of multidimensional blood flow through the heart chambers is fundamental in understanding the physiology of the heart and to detect abnormalities in cardiac function. The 4D Flow CMR parameters presented in this thesis can be utilized to detect altered intracardiac blood flow and may be used as markers of deteriorating cardiac function, pathological remodeling and mechanical dyssynchrony in heart failure.

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Andersson, Jim. « LEFT VENTRICULAR EJECTION FRACTION : A RETROSPECTIVE STUDY COMPARING 2D ECHOCARDIOGRAPHY AND GATED SINGLE PHOTON EMISSION COMPUTED TOMOGRAPHY (SPECT) IN CLINICAL USE ». Thesis, Uppsala University, Department of Medical Biochemistry and Microbiology, 2009. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-114139.

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Objectives

The aim of this study was to compare left ventricular ejection fraction (LVEF) results derived from gated single photon emission computed tomography (SPECT) using Cedars-Sinai quantitative gated SPECT (QGS) processing software with results from 2D echocardiography, both obtained in routine clinical diagnostic use.

Methods

Data from previously performed tests were obtained from 73 patients who had undergone both 2D echocardiography and gated SPECT within a time span of 6 months and had not had significant events that could influence LVEF. LVEF from 2D echocardiography was reassessed to obtain discrete values and then the data was compared using Bland-Altman analysis.

Results

The correlation between the tests was shown to be good, but precision lacked. Bland-Altman analysis showed a bias of -0.8 percentage points when gated SPECT compared to mean values and 2 standard deviations (SD) ranged from -20.2 to 18.6.

Conclusions

LVEF values from the two methods can differ quite a bit and comparisons between them should be done with great caution.

Syfte

Syftet med studien var att jämföra hur bra ultraljud av hjärta och isotopundersökning av hjärta stämmer överens när det gäller att visa hjärtats pumpförmåga.

Metod

Data från tidigare utförda undersökningar av 73 patienter jämfördes. Patienter som hade gjort båda undersökningarna inom 6 månader och under perioden mellan undersökningarna inte hade haft hjärtinfarkt eller någon annan händelse som kan påverka hjärtats pumpförmåga valdes till studien.

Resultat

Utslaget över hela studiepopulationen stämde resultaten från de båda undersökningarna bra överens. Jämförde man däremot resultaten från de båda undersökningarna med varandra patient för patient förekom mycket stora variationer.

Slutsats

Resultaten angående hjärtats pumpförmåga kan skilja sig mycket från varandra. Jämförelser av värden från dessa två metoder bör därför göras med väldigt stor försiktighet.

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Feldt, Olivia. « Evaluation of underfill-function in HemoCue Monitor, a POCT-instrument ». Thesis, Uppsala University, Department of Medical Biochemistry and Microbiology, 2006. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-6991.

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Objective: The aim of this study was to evaluate a new underfill-function in a POCT-instrument from HemoCue AB (Ängelholm, Sweden). The instrument is in use today among diabetes patients for self-monitoring blood glucose (SMBG). The new function is supposed to guarantee that measuring only will be performed on a sufficient sample volume to assure that the correct glucose value is received.

Methods and results: Blood samples (whole blood) from 12 patients were analysed with the instrument. Measuring were performed using different volumes in the cuvette. Full cuvette, 3µL, 2µL, 1µL and a measuring on an empty cuvette. The instrument performed measurements on all volumes added to the cuvette except for the empty cuvette. The less sample volume that was used the lower glucose values were reported by the instrument.

Conclusions: The new under fill-function did not work satisfactory. If such function would be more reliable it would be beneficial for the patient controlling hers/his bloodglucose provided that the testing procedure is being correctly done. This is very important because the results are often used to treat the patient.

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Björklund, Kristofer. « Evaluation and optimization of four real-time PCRs, using TaqMan-probes, for detection of and discrimination between barley, oat, rye and wheat ». Thesis, Uppsala University, Department of Medical Biochemistry and Microbiology, 2008. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-9171.

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Coeliac disease is a chronic inflammatory disease treated with a gluten-free diet, excluding barley, rye and wheat. Hence, there is a demand for methods able to detect gluten in foods in order to ensure correct labeling of products. According to the Codex Alimentarius Commission, 20ppm gluten is the maximum amount allowed in food labeled gluten-free.

PCR can detect DNA from cereals in food. Four real-time PCR-systems,

using TaqMan®-probes for detection of barley, oat, rye and wheat were optimized and evaluated. Evaluations were carried out using seeds. Primers were targeted to genes coding for prolamines, seed storage proteins. PCR-systems targeted to barley, oat and wheat were shown to be specific for the cereals corresponding to each system. The system targeted to rye showed cross-reactions with durum wheat and spelt wheat. Detection limits were 50pg, corresponding to <10 haploid genome copies for each cereal. All systems were able to detect 250ppm amounts of DNA, most likely even smaller amounts are detectable. All systems showed an amplification efficiency of ≥95%.

Systems for detection of barley, oat and wheat are ready for further evaluation, using food products as samples. The rye system however, needs to be re-designed before further evaluation can take place.

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Larsson, Madeleine. « When The Sentinels Fall : Macrophage Cell Death Response to GAS Infection ». Thesis, Uppsala universitet, Institutionen för medicinsk biokemi och mikrobiologi, 2017. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-348681.

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Group A Streptococcus (GAS) is a globally disseminated pathogen that causes >500,000 deaths yearly and is ranked as ninth leading infectious cause of human mortality by the World Health Organisation. The spectrum of disease ranges from superficial infections of the skin and epithelium to invasive and systemic infections. Although the interaction of GAS with neutrophils has been extensively studied much remains to be discovered about the role of macrophages, which are the first line of defence encountered by invading pathogens. In this study, the aim was to establish a means of deriving macrophages from primary monocytes and to study both the efficiency of macrophage killing of GAS and the macrophage cell death response to GAS infection. Here, we report that monocyte-derived macrophages are able to take up and kill GAS during in vitro infection. Production of reactive oxygen species by macrophages was elicited during infection, but not nearly in as high amounts as produced by neutrophils. Investigating the type of cell death induced by GAS, markers for both apoptosis and necroptosis can be found after 8 hours of infection. These results highlight that macrophages indeed are participating in the clearance of GAS and more studies are needed to understand the roles of macrophages in early control of GAS infection.

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Jäder, Klara. « Optimization of a multiplex ARMS-PCR for detection of the primary mutations causing Leber’s hereditary optic neuropath ». Thesis, Uppsala universitet, Institutionen för kvinnors och barns hälsa, 2020. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-413665.

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Leber’s hereditary optic neuropathy (LHON) is a genetic disease that causes the patients to become blind, first in one eye and then the other, around the ages of 10-75 years. The disease is caused by mutations in the mitochondrial DNA, which disturbs the respiratory chain leading to the deterioration of the retinal ganglion cells. This study’s aim is to optimize a multiplex amplification-refractory mutation system PCR for detection of three primary mutations causing LHON. This was done through a series of PCRs, including PCR aimed at the ß-globin gene, conventional simplex PCR and a simplex ARMS-PCR aimed at the three primary mutations causing LHON. This study was, however, terminated prematurely due the Covid-19 outbreak and the optimization of the ARMS-PCR could therefore not be done. This study’s aim was adapted to the new circumstances to instead provide guidance on how to perform the optimization using the results from the PCRs that were done before the termination. The results found that for the ARMS-PCR 2 mM of magnesium would suffice as a start point overall and the need to solve the problems with the two 14484 plasmids was evident. The ARMS-PCR is one of many methods that can be used to the detect single nucleotide polymorphism, but its availability and robustness makes this a method worth optimizing. To continue with the optimization of the ARMS-PCR several factors would have to be tested, including annealing temperature, primer concentrations and magnesium concentration.

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Cedergren, Linda. « Expression of recombinant protein including an His-tag to facilitate purification for diagnosis of CCHF and Lassa Viruses ». Thesis, Uppsala University, Department of Medical Biochemistry and Microbiology, 2006. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-7064.

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Abstract

Crimean-Congo Hemorrhagic Fever virus (CCHF) and Lassa virus are giving sources illness to humans. In addition to zoonotic transmission, CCHF and Lassa virus can spread from person to person. After a short incubation period, CCHF and Lassa virus infections are characterized by a sudden onset of high fever, chills, headache and cough just like flu. Even some people are vomiting and have diarrhoea. After a few days of illness hemorrhagic manifestations occur. Treatment options for CCHF and Lassa viruses are limited, and there is no vaccine available for use in humans. The purpose of the present study was to produce recombinant nucleocapsid protein of Lassavirus and CCHF virus including an aminoterminal His-tag by a Semliki Forest Virus Replicon (pSFV 4.2). The recombinant proteins are planned to be used in future development of diagnostic methods.

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Ekelund, Emil. « LactateStat : Wearable Electronics and Software for Real-Time Lactate Monitoring in Sweat ». Thesis, KTH, Medicinteknik och hälsosystem, 2021. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-297538.

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Lactate is an important biomarker in sports and the lactate threshold concept is one of the best indicators of endurance performance in an athlete. However, to quantify the lactate threshold, an invasive method to take a blood sample must be used. Limitations for this method include stopping the active exercise for blood sampling and no real-time feedback. Instead, a novel non-invasive wearable biosensor can be used to measure the lactate concentration in sweat. The sensor generates an electrical current depending on the lactate concentration in the sweat, and therefore must an electronic device called a potentiostat be used to condition the current. However, available potentiostats are not suitable for use in sports where form factor, battery life and wireless connectivity is important. This thesis aims to solve this by developing a wearable device and software which can be used to measure the lactate concentration in sweat in real-time during exercise. The development process consisted of the determination of specifications, prototype development and thorough laboratory and on-body testing. Finally, a novel wearable device and software capable of real-time lactate measurements in sweat during exercise were presented. The device, called LactateStat, was 58mm ∗ 55mm ∗ 13mm, the current consumption was only 7.8mA, the current measurement resolution was 0.5 nA, the limit of detection was 0.45 nA and the current measurement range was around 750 μA. LactateStat is one of the first of its kind and provides a base for future development as the hardware, firmware and software resources are provided as open-source.
Laktat är en viktig biomarkör inom idrott och konceptet laktattröskel är en av de bästa indikatorerna för uthållighet hos en idrottsutövare. Men för att mäta laktattröskeln krävs en invasiv metod som går ut på att man tar ett blodprov. Begränsningarna med denna metod är bland annat att idrottsutövningen måste stoppas för att ta ett blodprov och att man inte får realtidsåterkoppling. I stället kan en liten och icke-invasiv bärbar biosensor användas för att mäta laktatkoncentrationen i svett. Sensorn genererar en elektrisk ström beroende på laktatkoncentrationen i svetten och en enhet som kallas potentiostat måste därför användas för att mäta denna ström. Tillgängliga potentiostater är dock inte lämpliga för användning inom sport där formfaktor, batteritid och trådlös kommunikation är viktigt. Denna avhandling har som syfte att lösa detta genom att utveckla en bärbar enhet och mjukvara som kan användas för att mäta laktatkoncentrationen i svett i realtid under träning. Utvecklingsprocessen bestod av bestämning av specifikationer, framtagning av en prototyp och noggrann laboratorie- och kroppstestning. Som resultat presenterades en banbrytande bärbar enhet och mjukvara som kan mäta laktat i realtid i svett under träning. Enheten, som kallades LactateStat, var 58mm * 55mm * 13mm, den aktiva strömförbrukningen var 7.8mA, den bästa mätupplösningen för ström var 0.5 nA, detektionsgränsen var 0.45 nA och det maximala mätområdet för ström var ca. 750 μA. LactateStat är en av de första i sitt slag och ger en bas för framtida utvecklingsarbete eftersom hårdvaru-, programvaru- och mjukvaruresurserna tillhandahålls som öppen källkod.

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Immonen, K. (Kati). « Continuing professional development of medical laboratory professionals ». Bachelor's thesis, University of Oulu, 2018. http://urn.fi/URN:NBN:fi:oulu-201805221864.

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The aim of the bachelor study was to find out what kind of experiences continuing professional development (CPD) has received in medical laboratories and how it has been implemented through a literature review. The purpose of the study was to find and compile the literature review as up-to-date research data on the importance of continuing education in medical laboratories. The literature review has been carried out by adapting a systematic literature review. The literature review material was obtained utilizing nursing reference databases from CINAHL, PubMed, Ovid Medline, Scopus, Medic and a few international journals. Research material (n = 8) was selected for the material, whose main subject was continuing professional development and medical laboratory. CPD is used to improve knowledge and skills with various categories and tools but there is similarities and differences in CPD implementation. CPD must be continuous throughout the professional career and it should be based on time- cycles of reasonable duration. Medical laboratory professionals with dozens of years of experience feels that they need less CPD activities than recently qualified laboratory professionals. The laboratory professional keeps important to increase their learning and development of their skills with continual encouragement. Research has shown that CPD has been recognized as an important part of professional development and the use of various tools, such as internet-based, makes it effective. The use of a systematic CPD program should be constantly evaluated to identify the most effective ways of developing professional skills. CPD should be time bounded but there is variation in duration and volume. The use of time and experience years will affect the successful implementation of the CPD.

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Lourenço, Patrick Menezes. « Erros pré-analíticos em medicina laboratorial : uma revisão sistemática ». Universidade do Estado do Rio de Janeiro, 2013. http://www.bdtd.uerj.br/tde_busca/arquivo.php?codArquivo=8244.

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MENEZES, Patrick Lourenço. Erros pré-analíticos em medicina laboratorial: uma revisão sistemática. 2013. 98 f. Dissertação (Mestrado em Saúde, Medicina Laboratorial e Tecnologia Forense) - Instituto de Biologia Roberto Alcântara Gomes, Universidade do Estado do Rio de Janeiro, Rio de Janeiro, 2013. A relevância evidente dos erros pré-analíticos como problema de saúde pública fica patente tanto no dano potencial aos pacientes quanto nos custos ao sistema de saúde, ambos desnecessários e evitáveis. Alguns estudos apontam que a fase pré-analítica é a mais vulnerável a erros, sendo responsável por, aproximadamente, 60 a 90% dos erros laboratoriais em consequência da falta orientação aos pacientes sobre os procedimentos que serão realizados no laboratório clínico. Objetivos: Sistematizar as evidências científicas relacionadas aos erros pré-analíticos dos exames laboratoriais de análises clínicas. Método: Uma revisão sistemática foi realizada, buscando as bases de dados do Medical Literature Analysis and Retrieval System Online (MEDLINE), Scopus(que inclui MEDLINE e Embase), ISI Web of Knowledge, SciFinder, Literatura Latino-Americana e do Caribe em Ciências da Saúde (Lilacs) (que inclui a Scientific Electronic Library Online SciELO) e o Índice Bibliográfico Espanhol de Ciências de Saúde (IBECS), para artigos publicados entre janeiro de 1990 e junho de 2012 sobre erros de exames laboratoriais que possam ocorrer na fase pré-analítica. Os estudos foram incluídos de acordo com os seguintes exames laboratoriais: hemograma, análise bioquímica do sangue total ou do soro, exames de coagulação sanguínea,uroanálise e exames hematológicos ou bioquímicos em outros materiais e categorizados pelo tipo de erro pré-analítico e pela frequência dos incidentes. Resultados: A busca nas bases de dados bibliográficas resultou no seguinte número de artigos recuperados: 547 na MEDLINE, 229 na Scopus, 110 na ISI, 163 na SciFinder, 228 na Lilacs e 64 na IBECS, perfazendo um total de 1.341 títulos. Ao fim da revisão sistemática, obteve-se um conjunto de 83 artigos para leitura de texto completo, dos quais 14 foram incluídos na revisão. Os estudos abrangeram diferentes tipos de laboratórios, setores técnicos e origem de erros, segundo a fase do processo laboratorial. Discussão: Sete artigos demonstraram erros de pedidos médicos, com uma alta variabilidade nos valores de incidência. Os seis artigos que estudaram erros de coleta de amostra observaram redução deste desfecho. As proporções de eventos adversos relatados e os impactos clínicos variaram, levando a consequências descritas como: erros decorrentes da flebotomia, recoleta de amostras, repetições de exames, atrasos na liberação de resultados de exames e possíveis danos ao paciente. Conclusões: O laboratório deve ter instruções por escrito para cada teste, que descreva o tipo de amostra e procedimento de coleta de amostra. Meios de identificação por código de barras, sistemas robóticos e analíticos reduzem os erros pré-analíticos. A melhoria da fase pré-analítica de testes laboratoriais permanece um desafio para muitos laboratórios clínicos.
The obvious relevance of preanalytical errors as a public health problem is clear in both the potential harm to patients and cost to the health system, both unnecessary and avoidable. Some studies indicate that the pre-analytical phase is the most vulnerable to errors, accounting for approximately 60-90% of laboratory errors as a result of lack guidance to patients about the procedures to be performed in the clinical laboratory. Objectives: To systematize the scientific evidence related to preanalytical errors of clinical analysis laboratory. Method: A systematic review was conducted, searching the databases of the Medical Literature Analysis and Retrieval System Online (MEDLINE) , Scopus (which includes MEDLINE and Embase ), ISI Web of Knowledge , SciFinder , Latin American and Caribbean Health Sciences (LILACS) ( which includes the Scientific Electronic Library Online - SciELO) and the Spanish Bibliographic Index of Health Sciences (IBECS) for articles published between January 1990 and June 2012 on laboratory errors that may occur in the preanalytical phase. Studies were included according to the following laboratory tests: complete blood count, biochemical analysis of whole blood or serum, blood coagulation tests, urinalysis and hematological or biochemical analysis of other materials categorized by the type of pre - analytical error and the frequency of incidents. Results: The search in bibliographic databases resulted in the following number of items retrieved: 547 in MEDLINE, Scopus at 229, 110 in the ISI in SciFinder 163, 228 and 64 in the Lilacs IBECS, a total of 1.341 titles. At the end of the systematic review, we obtained a set of 83 articles for reading the full text, of which 14 were included in the review. The studies covered different types of laboratories, technical sectors and source of errors, according to the phase of the laboratory process. Discussion: Seven articles showed errors in the medical requisition, with a high variability in the incidence values. The six articles that studied sample collection errors observed reduction in this outcome. The proportions of reported adverse events and clinical impacts varied, leading to consequences described as: errors resulting from phlebotomy, recollection of samples, repetition of exams, delays in the release of the test results and possible harm to the patient. Conclusions: The laboratory must have written instructions for each test, which describes the type of sample and collection procedure. Identification methods by barcode, robotic and analytical systems, reduce preanalytical errors. The improvement of pre-analytical phase of laboratory tests remains a challenge for many clinical laboratories.

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Dussauge, Isabelle. « Technomedical Visions : Magnetic Resonance Imaging in 1980s Sweden ». Doctoral thesis, Stockholm : Filosofi och teknikhistoria, Philosophy and the History of Technology, Kungliga Teknsika högskolan, 2008. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-4671.

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Bin, Kaderi Mohamed Arifin. « Assessment of Novel Molecular Prognostic Markers in Chronic Lymphocytic Leukemia ». Doctoral thesis, Uppsala universitet, Hematologi och immunologi, 2010. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-110371.

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The clinical course of chronic lymphocytic leukemia (CLL) is highly heterogeneous, which has prompted the search for biomarkers that can predict prognosis in this disease. The IGHV gene mutation status and certain genomic aberrations have been identified as reliable prognostic markers of clinical outcome for this disorder. However, the search for more feasible prognostic markers in CLL is still being pursued. Recently, certain single nucleotide polymorphisms (SNPs) in the GNAS1, BCL2 and MDM2 genes and the RNA expression levels of the LPL, ZAP70, TCL1, CLLU1 and MCL1 genes were suggested as novel prognostic markers in CLL. In papers I-III, we performed genotyping analyses of the GNAS1 T393C, BCL2 -938C>A and MDM2 SNP309 polymorphisms in 268-418 CLL patients and related the genotypes with clinical data. Association studies between the polymorphisms and established prognostic markers (i.e. IGHV mutation status, genomic aberrations, CD38 expression) were also performed. Our studies did not find any significant relationship between these SNPs with either clinical outcome or other known prognostic markers in CLL. In paper IV, we measured the RNA expression levels of LPL, ZAP70, TCL1, CLLU1 and MCL1 in 252 CLL cases and correlated these levels with clinical outcome. Here, we verified that high expression of all these RNA-based markers, except MCL1, were associated with an unfavourable prognosis. We also confirmed a close relationship between IGHV mutation status and the RNA-based markers, especially for LPL and CLLU1 expression. Among the RNA-based markers, multivariate analysis revealed LPL expression as the strongest independent prognostic marker for overall survival and time to treatment. Furthermore, the RNA-based markers could add further prognostic information to established markers in subgroups of patients, with LPL expression status giving the most significant results. In summary, data from papers I-III could not verify the GNAS1 T393C, BCL2 -938C>A and MDM2 SNP309 polymorphisms as prognostic markers in CLL. Future SNP markers must hence be confirmed in large, independent cohorts before being proposed as prognostic marker in CLL. In paper IV, we conclude that LPL expression appears to be the strongest among the RNA-based markers for CLL prognostication. Further efforts to standardize LPL quantification are required before it can be applied in the clinical laboratory to predict clinical outcome in this disease.

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Kurt, Nour, et Jonna Sohlé. « Jämförelse mellan Grocotts manuella och automatiserade färgningsmetod ». Thesis, Hälsohögskolan, Högskolan i Jönköping, HHJ, Avd. för naturvetenskap och biomedicin, 2017. http://urn.kb.se/resolve?urn=urn:nbn:se:hj:diva-36077.

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Grocott metoden är den känsligaste metoden för infärgning av svampstrukturer i histologisk vävnad i jämförelse med till exempel Periodic Acid-Schiff (PAS). Handhavandet av det cancerogena oxidationsmedlet kromsyra samt tidsaspekten för Grocott är signifikanta problem. Syftet med studien var att undersöka om den automatiserade färgningsmetoden för Grocott ger likvärdiga resultat som vid den manuella färgningsmetoden. Tolv preparat färgades med både den manuella och den automatiserade metoden för Grocott. Den automatiserade färgningsmetoden använder ett färdigt kit med alla ingående lösningar. Visuell bedömning av preparaten utfördes med hjälp av legitimerade biomedicinska analytiker samt en patolog. Svampstrukturer i åtta av ela preparat samt kontroll bedömdes vara tydligare infärgade med den automatiserade metoden i färhållande till den manuella. Svampstrukturer i endast ett preparat bedömdes vara tydligare infärgade med den manuella metoden. Resterande två preparat visade inga svampstrukturer. Resultaten tyder på att den automatiserade färgningsmetoden för Grocott ger likvärdiga, alternativt tydligare infrägning av svampstrukturer i jämförelse med den manuella metoden.
The Grocott method is the most sensitive staining method for fungal structures in histological tissues in comparison to for example Periodic Acid-Schiff (PAS). Usage of the cancerogenic chromic acid and the time aspect for Grocott are significant problems. The aim of this study was to examine if the automated staining method for Grocott provides equivalent results in comparison to the manual staining method. Twelve tissue sections were stained with both the automated and the manual method. The automated method contains a kit with all solutions. Visual evaluation was conducted with authorized biomedical scientists and a pathologist. Fungi structures in eight of eleven tissue sections and a control were evaluated as more distinct in the automated method in comparison to the manual method. Fungal structures in one of the tissue sections were evaluated more distinct in the manual method. In the remaining two tissue sections, no fungal structures were identified. Results from this study indicates that the automated staining method for Grocott provides equally, alternatively more distinct fungal staining in comparison to the manual method.

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Korkis, Layal. « Nanoparticles’ effect in an in vitro whole blood model ». Thesis, Uppsala universitet, Institutionen för immunologi, genetik och patologi, 2019. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-392201.

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Nanoparticles have been used in industry and in medicine due to their properties which give them beneficial uses. This usage of the nanoparticles has risen the question about how harmful they are to the human body, the connection between the exposure to nanoparticles, and many diseases that occur in the body. Methods This study focused on the effect of nanoparticles in a whole human blood loop model. The blood was incubated with Silica, Titanium dioxide and Palladium particles in heparinized loops without any anticoagulants added. The blood’s cell count was analyzed with a cell counter and then complement, and contact system’s markers were analyzed with ELISA to detect a presence of activations in the systems. Experiments one to five were an optimization of test settings. Results An activation of the contact system was initiated in the loops containing the aggregated titanium dioxide nanoparticles. A high platelets consumption up to 73.8 % was observed as well as two visible clots. On top of that, blood smears showed micro-clots in the blood incubated with the aggregated nanoparticles. Conclusion Nanoparticles initiated an activation in the contact system in the aggregated form in comparison with the dispersed form. Further and deeper studies should be executed to observe the importance of the single or the aggregated form in the actual effect on the immune system.

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West, Julie Ann. « Factors Associated With Tuberculin Skin Test Positivity Prevalence in U.S. Medical Laboratory Microbiologists ». Thesis, Walden University, 2014. http://pqdtopen.proquest.com/#viewpdf?dispub=3607454.

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Prior research has indicated that healthcare personnel (HCP) who work in areas where Mycobacterium tuberculosis poses an occupational hazard are at high risk of tuberculin skin test (TST) positivity and subsequent conversion to active tuberculosis (TB). U.S. medical laboratory microbiologists confront similar hazards but have not been studied outside of the HCP aggregate. The purpose of this study was to fill this gap by examining the relationships between the predictor variables of self-reported history of bacille Calmette-Guérin (BCG) immunization, place of birth, and years of laboratory experience and the outcomes of self-reported lifetime TST positivity, preventive treatment noninitiation, and barriers to treatment adherence for this subgroup. This quantitative, cross-sectional study was guided by the epidemiologic triad model. A researcher-designed self-administered questionnaire including Part A of the Brief Medication Questionnaire was mailed to 4,335 U.S. microbiologist members of the American Society for Clinical Pathology. From the 1,628 eligible respondents, results showed that prevalence of positive TSTs (17.0%) and treatment noninitiation (9.8%) was low. Multivariate analysis identified BCG and foreign birth, as well as age, nonoccupational exposure, history of TB, work in mycobacteriology, and work outside of microbiology as predictors of a positive TST; foreign birth was a predictor of treatment noninitiation. Additional research is needed to identify other laboratorian groups at increased risk for developing TB. These results enhance positive social change by helping to inform recommendations in the global fight to stop the spread of TB, as well as improve allocation of resources among this specific group of HCP.

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Manivasagam, Karnica. « COMPARISON OF WRIST VELOCITY MEASUREMENT METHODS : IMU, GONIOMETER AND OPTICAL MOTION CAPTURE SYSTEM ». Thesis, KTH, Medicinteknik och hälsosystem, 2020. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-287178.

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Repetitive tasks, awkward hand/wrist postures and forceful exertions are known risk factors for work-related musculoskeletal disorders (WMSDs) of the hand and wrist. WMSD is a major cause of long work absence, productivity loss, loss in wages and individual suffering. Currently available assessment methods of the hand/wrist motion have the limitations of being inaccurate, e.g. when using self-reports or observations, or expensive and resource-demanding for following analyses, e.g. when using the electrogoniometers. Therefore, there is a need for a risk assessment method that is easy-to-use and can be applied by both researchers and practitioners for measuring wrist angular velocity during an 8-hour working day. Wearable Inertial Measurement Units (IMU) in combination with mobile phone applications provide the possibility for such a method. In order to apply the IMU in the field for assessing the wrist velocity of different work tasks, the accuracy of the method need to be examined. Therefore, this laboratory experiment was conducted to compare a new IMU-based method with the traditional goniometer and standard optical motion capture system. The laboratory experiment was performed on twelve participants. Three standard hand movements, including hand/wrist motion of Flexion-extension (FE), Deviation, and Pronationsupination (PS) at 30, 60, 90 beat-per-minute (bpm), and three simulated work tasks were performed. The angular velocity of the three methods at 50th and 90th percentile were calculated and compared. The mean absolute error and correlation coefficient were analysed for comparing the methods. Increase in error was observed with increase in speed/bpm during the standard hand movements. For standard hand movements, comparison between IMUbyaxis and Goniometer had the smallest difference and highest correlation coefficient. For simulated work tasks, the difference between goniometer and optical system was the smallest. However, for simulated work tasks, the differences between the compared methods were in general much larger than the standard hand movements. The IMU-based method is seen to have potential when compared with the traditional measurement methods. Still, it needs further improvement to be used for risk assessment in the field.
Upprepade uppgifter, besvärliga hand- / handledsställningar och kraftfulla ansträngningar är kända riskfaktorer för arbetsrelaterade muskuloskeletala störningar (WMSD) i hand och handled. WMSD är en viktig orsak till lång frånvaro, produktivitetsförlust, löneförlust och individuellt lidande. För närvarande tillgängliga bedömningsmetoder för hand / handledsrörelser har begränsningarna att vara felaktiga, t.ex. när du använder självrapporter eller observationer, eller dyra och resurskrävande för följande analyser, t.ex. när du använder elektrogoniometrarna. Därför finns det ett behov av en riskbedömningsmetod som är enkel att använda och som kan användas av både forskare och utövare för att mäta handledens vinkelhastighet under en 8-timmars arbetsdag. Wearable Inertial Measuring Units (IMU) i kombination med mobiltelefonapplikationer ger möjlighet till en sådan metod. För att kunna använda IMU i fältet för att bedöma handledens hastighet för olika arbetsuppgifter måste metodens noggrannhet undersökas. Därför genomfördes detta laboratorieexperiment för att jämföra en ny IMU-baserad metod med den traditionella goniometern och det vanliga optiska rörelsefångningssystemet. Laboratorieexperimentet utfördes på tolv deltagare. Tre standardhandrörelser, inklusive hand / handledsrörelse av Flexion-extension (FE), Deviation och Pronation-supination (PS) vid 30, 60, 90 beat-per-minut (bpm) och tre simulerade arbetsuppgifter utfördes. Vinkelhastigheten för de tre metoderna vid 50: e och 90: e percentilen beräknades och jämfördes. Det genomsnittliga absoluta felet och korrelationskoefficienten analyserades för att jämföra metoderna. Ökning av fel observerades med ökning av hastighet/bpm under standardhandrörelserna. För standardhandrörelser hade jämförelsen mellan IMUbyaxis och Goniometer den minsta skillnaden och högsta korrelationskoefficienten. För simulerade arbetsuppgifter var skillnaden mellan goniometer och optiskt system den minsta. För simulerade arbetsuppgifter var dock skillnaderna mellan de jämförda metoderna i allmänhet mycket större än de vanliga handrörelserna. Den IMUbaserade metoden anses ha potential jämfört med traditionella mätmetoder. Ändå behöver det förbättras för att kunna användas för riskbedömning på fältet.

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Small, Kathy S. « Retention Strategies for Medical Technologists : Addressing the Shortages and Vacancies in the Clinical Laboratory ». Digital Commons @ East Tennessee State University, 2013. https://dc.etsu.edu/etd/2299.

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It is important to have well-trained and qualified laboratory professionals. Seventy percent of patient care is based on decisions made from laboratory results, yet there is a growing shortage of medical technologists. Although some baby boomers are delaying retirement, worsening of the shortage crisis is inevitable. Retention of medical technologists has become more important than recruitment. The purpose of this study was to identify and evaluate innovative retention strategies used by clinical laboratory managers throughout the United States. A significant finding of this study was the lack of qualified medical technologists entering the ranks of laboratory managers. This study identified a need for a more defined career path and more recognition of the importance of laboratory scientists. It is recommended that studies be undertaken to examine the opinions of hospital and medical group practice administrators as well as the view of medical technologists regarding retention strategies that are proven to be effective.

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Åstrand, Bengt. « ePrescribing : Studies in Pharmacoinformatics ». Doctoral thesis, Högskolan i Kalmar, Naturvetenskapliga institutionen, 2007. http://urn.kb.se/resolve?urn=urn:nbn:se:hik:diva-32.

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Det övergripande syftet med den här avhandlingen har varit att, inom området läkemedelsinformatik, studera utvecklingen av elektroniska stöd inom läkemedelsförskrivning; för klinisk praxis, uppföljning och forskning. Under århundraden har det handskrivna receptet varit det sätt, med vilket läkare förmedlat sina läkemedelsordinationer till apotekare, vilket också för patienten blivit en informationskälla för hur läkemedel ska användas för att göra bästa nytta. Nu genomgår receptet en förändring från pappersbaserat till elektroniskt meddelande och att anpassa en traditionell process till en ny elektronisk era innebär både möjligheter och utmaningar. Studierna som ingår i avhandlingen har visat att exponeringen av förskrivna läkemedel i en allmän befolkning har ökat under de senaste tre decennierna. Risken för potentiella interaktioner mellan läkemedel, varmed avses den risk som finns att olika läkemedel kan påverka varandras effekter och biverkningar, har också visat sig öka starkt desto fler läkemedel som används av en individ. Denna ökade samtidiga användning av flera olika läkemedel, så kallad polyfarmaci, medför att det finns en större anledning för förskrivare och farmacevter att uppmärksamma risken för potentiella interaktioner mellan läkemedel. De nyinrättade nationella receptregistren över uthämtad receptförskriven medicin bör användas bland annat för att upptäcka potentiella läkemedelsinteraktioner, såväl i vårdens utövning som inom läkemedelsepidemiologisk forskning. Den svenska läkemedelsförteckningen, som omfattar information om uthämtade receptförskrivna läkemedel för huvuddelen av den svenska befolkningen, bedöms ha en stor klinisk potential. Den enskilde individens historiska information om uthämtade läkemedel är tillgänglig för individen på Internet med hjälp av e-legitimation; även förskrivare och farmacevter på apotek kan ta del av informationen med den enskildes samtycke. Brist på tillgång till enhetliga och säkra autenticeringsmetoder inom hälso- och sjukvården kan dock fördröja tillgången på individuell läkemedelsinformation för förskrivare. I och med att de flesta recepten i Sverige nu skrivs och överförs elektroniskt är det viktigt att kvalitetsmässiga aspekter tas tillvara så att en iakttagen ökad risk för receptförskrivningsfel inte överförs i informationskedjan. Avhandlingens slutsats är att e-förskrivning, med kommunikation och användning av lagrad information om receptexpeditioner, möjliggör att läkemedelsbehandling som process kan följas och studeras på ett helt nytt
The thesis aimed to study the developments, in the area of pharmacoinformatics, of the electronic prescribing and dispensing processes of drugs - in medical praxis, follow-up, and research. For hundreds of years, the written prescription has been the method of choice for physicians to communicate decisions on drug therapy and for pharmacists to dispense medication. Successively the prescription has also become a source of information for the patient about how to use the medication to maximize its benefit. Currently, the medical prescription is at a transitional stage between paper and web, and to adapt a traditional process to the new electronic era offers both opportunities and challenges The studies in the thesis have shown that the exposure of prescribed drugs in the general population has increased considerably over three decades. The risk of receiving potentially interacting drugs was also strongly correlated to the concomitant use of multiple drugs, polypharmacy. The pronounced increase in polypharmacy over time constitutes a growing reason for prescribers and pharmacists to be aware of drug interactions. Still, there were relatively few severe potential drug interactions. Recently established national prescription registers should be evaluated for drug interaction vigilance, both clinically and epidemiologically. The Swedish National Pharmacy Register provides prescription dispensing information for the majority of the population. The medication history in the register may be accessed online to improve drug utilization, by registered individuals, prescribers, and pharmacists in a safe and secure way. Lack of widespread secure digital signatures in healthcare may delay general availability. With a relatively high prevalence of dispensed drugs in the population, the National Pharmacy Register seems justified in evaluating individual medication history. With a majority of prescriptions transferred as ePrescriptions, the detected increased risk for prescription errors warrants quality improvement, if the full potential of ePrescriptions is to be fulfilled. The main conclusion of the studies was that ePrescribing with communication of prescribed drug information, storing and retrieving dispensed drug information, offers new opportunities for clinical and scientific

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Linde, Ida, et Caroline Svedberg. « Misfit – Impact on Porcelain Fracture and Screw Loosening of Implant-supported FDPs - A Laboratory Pilot Study ». Thesis, Malmö högskola, Odontologiska fakulteten (OD), 2017. http://urn.kb.se/resolve?urn=urn:nbn:se:mau:diva-19948.

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Syfte: Syftet med denna laborativa pilotstudie var att undersöka huruvida en misfit mellan ettimplantat och en skruvretinerad bro ökade risken för sprickor och chip-off frakturer i porslinet, samt risken för uppskruvning av implantatskruvarna.Material och metod: Tjugo stycken implantatstödda skruvretinerade femledsbroar tillverkades i titan med ytporslin. Provkropparna fördelades lika mellan en testgrupp och en kontrollgrupp. I testgruppen skapades en misfit på 150 μm mellan bron och implantatet vid position 5.Provkropparna genomgick artificiellt åldrande i en cyklisk belastningsmaskin i 100 000 cykler.Synliga sprickor och chip-off frakturer noterades under och efter testet och skruvarna kontrollerades gällande uppskruvning.Resultat: Synliga sprickor i porslinet uppstod signifikant mer frekvent i testgruppen i jämförelse med kontrollgruppen. Nio broar i testgruppen uppvisade sprickor jämfört med en bro i kontrollgruppen. Tre chip-off frakturer uppstod i testgruppen jämfört med noll i kontrollgruppen, skillnaden var inte statistiskt signifikant. Vid kontroll av implantatskruvarna sågs inga signifikanta skillnader mellan varken implantatpositioner eller grupper gällande retorque-värde. Inga av skruvarna var lösa.Slutsats: Inom denna studies begränsningar tyder resultaten på att närvaron av en misfit mellan implantat och överkonstruktion kan öka risken för sprickbildning och/eller chip-off frakturer. Vidare studier krävs för att bekräfta dessa resultat.
Aim: The aim of this laboratory pilot study was to investigate whether a misfit between an implant and a FDP increase the risk of cracks, chip-off fractures and screw loosening of screw-retained implant-supported FDPs.Material and Method: Twenty screw-retained five-unit implant-supported FDPs were made intitanium with veneering porcelain. The specimens were evenly distributed into a test and control group. In the test group a misfit of 150 μm was created between the implant and FDP at position 5. All specimens underwent artificial aging in a cyclic loading machine for 100 000 cycles. Visible cracks and chip-off fractures were recorded during and after the test and the specimens were controlled for screw loosening.Results: Visible cracks within the porcelain veneer occurred significantly more often in the test group compared to the control group. Nine FDPs in the test group presented visible cracks, compared to one in the control group. Three chip-off fractures were recorded in the test group, none in the control group. This difference was not statistically significant. The retorque values of the screws presented no statistical significant differences neither between the implant positions nor the groups. None of the screws in the groups were loose.Conclusion: Within the limitations of this pilot study the results indicate that the presence of amisfit may increase the risk of cracking and/or chip-off fractures. Further studies are needed to confirm these findings.

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Qasemi, Adel, et Radhi Zayny. « The effect of preoperative brushing with chlorhexidine gel on bacterial contamination of bone transplant A clinical laboratory study ». Thesis, Malmö högskola, Odontologiska fakulteten (OD), 2016. http://urn.kb.se/resolve?urn=urn:nbn:se:mau:diva-19855.

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Introduktion: Förlust av tänder är idag vanligt förekommande och kan behandlas med hjälp av dentala implantat. För att installation av implantat ska vara möjlig, krävs tillräcklig benvolym där implantat ska installeras. När det föreligger bendefekter i anslutning till det lokala området för implantatinstallationen utgör simultaneous augmentation technique eller enstegsteknik ett behandlingsalternativ för bentransplantat. För att minimera kontamination med orala bakterier av det uppsamlade benet under beninsamlingsmetoder, används idag preoperativ sköljning med klorhexidin som ett steg i behandlingen.Syftet med denna studie är att undersöka möjligheten till ytterligare minimering av bakteriell kontamination vid implantatinstallation, med hjälp borstning av munhålan med klorhexidingel.Metod: 30 patienter deltog i en dubbelblindad, randomiserad studie där 15 patienter utgjorde en kontrollgrupp och 15 andra patienter fick genomgå den införda preoperativa tilläggsåtgärden. Ben- och paperpoint prov togs från samtliga patienter för bakteriell odling. Antalet kontaminerande bakterier uttrycktes som kolonibildande enheter (CFU/ml). En statistisk analys gjordes för att beräkna bakterieantal och kontaminationsgraden i respektive prov. Resultat: Medelvärdet för antal bakterier i benproven från samtliga patienter i kontrollgruppen beräknades till 8 126 CFU/ml respektive 2 946 CFU/ml i testgruppen dock var skillnaden inte signifikant (P = 0,357).Konklusion: Resultatet i denna studie visar ingen statistisk signifikant skillnad mellan patienter som erhållit den preoperativa profylaktiska tilläggsåtgärden, borstning med klorhexidingel, och patienter som inte erhållit detta hygiensupplement. Dessutom kunde ingen slutsats dras gällande effekten av denna tilläggsmetod på virulenta bakteriearterna P. gingivalis, P. Intermedia och A. actinomycetemcomitans. Ett större patientunderlag krävs i framtida randomiserade kontrollerade studier för att utvärdera denna metods effekt.
Introduction: Missing teeth can be replaced by dental implants. To achieve good results using dental implants, it is important that there is sufficient bone volume. In cases with bone defects, simultaneous augmentation technique becomes an alternative for bone augmentation. To minimize the contamination with oral bacteria during the bone collection, preoperative rinsing with chlorhexidine is today commonly used as a step in the treatment.The aim of this study is to examine the additional effect of preoperative brushing with chlorhexidine gel on avoiding bacterial contamination during the installation of dental implants.Method: 30 patients participated in a randomized, double blinded study. 15 patients were included in the control group and 15 patients underwent an additional step in the treatment with a preoperative brushing with chlorhexidine gel. Bone samples and paperpoint samples were collected and analyzed in a laboratory and a statistic analysis was performed to compare the number of bacterial colonies in samples from the two groups (CFU/ml) present and the degree of contamination.Results: The mean bacterial count in bone samples from all patients in the control group was calculated at 8 126 CFU/ml and 2 946 CFU/ml in the test group. CFU/ml was different between the groups (P = 0.357) but the difference was not significant (P <0.05).Conclusion: Brushing with chlorhexidine gel preoperatively showed no significant effects in the bacterial contamination of bone chips. No recommendation for adding this additional step in the standard treatment with dental implants can therefore be made today.

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Hosseini, Mona. « Genome-wide DNaseI hypersensitive sites profiles in laboratory mouse strains by DNase-seq ». Thesis, University of Oxford, 2013. http://ora.ox.ac.uk/objects/uuid:c76109fc-93b5-4e0b-b7df-0277cbf527a9.

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Variation at regulatory elements, identified through hypersensitivity to digestion by Deoxyribonuclease I (DNase I), is believed to contribute to variation in complex traits, but the extent and consequences of this variation are poorly characterized. To investigate the relationship between sequence variation, and the functional consequences of variation in chromatin accessibility, genome-wide DNase I hypersensitive sites (DHS) of terminally differentiated erythroblasts were studied in eight inbred strains of mice studied (A/J, AKR/J, BALBc/J, C3H/HeJ, C57BL/6J, CBA/J, DBA/2J, and LP/J). These strains were selected because of the availability of their genome sequence and quantitative trait loci (QTL) data. After confirming that next generation sequencing could identify DNase I hypersensitive sites with high sensitivity and specificity, and that differential peaks could be found, an automated peak calling pipeline was developed and optimized. 36,693 DHS peaks were identified covering 9.1 Mb (0.29%) of mouse genome. There was no indication of within strain variation. Between strains reproducible variation was observed at approximately 5% of DNase hypersensitive sites (1,397 DHSs). Variable DHSs were more likely to be enhancers than promoters and less likely to occur at conserved regions of the genome. Only 36% of such variable DHSs contain a sequence variant predictive of site variation and 12% contain at least one variant that disrupts transcription factor binding sites. The majority (86%) of variable DHSs differ in size/shape and the remaining 14% demonstrate discrete variation in single peak or cluster of peaks. Sequence variants within variable DHS are more likely to be associated with complex traits than those in non-variant DHS, and variants associated with complex traits preferentially occur in enhancer-like elements. Changes at a small proportion (7%) of discretely variable DHS are associated with changes in nearby transcriptional activity. Our results show that whilst DNA sequence variation is not the major determinant of variation in open chromatin, where such variants exist they are likely to be causal for complex traits.

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Rashid, Soma, et Susann Khalil. « En studie om fem tandtekniska laboratorier i Malmö, utifrån ett arbetsmiljöperspektiv ». Thesis, Malmö högskola, Odontologiska fakulteten (OD), 2017. http://urn.kb.se/resolve?urn=urn:nbn:se:mau:diva-19773.

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Inledning: Arbetsmiljön är en viktig aspekt att ta hänsyn till för att undvika skador. Det är allt från ergonomiska skador till sjukdomar som kan förhindras genom att använda sig av rätt teknik och skyddsutrustning. En annan viktig del inom tandteknikeryrket är att laboratorierna måste ta ansvar för sina handlingar, till exempel val av material och materialhantering samt fokus på arbetstagarnas säkerhet. Slutligen för att kunna uppnå ett optimalt resultat krävs det att laboratorierna är kvalitetssäkrade. Syfte: Syftet med arbetet är att undersöka hur tandtekniska arbetsplatser ser ut, utifrån ett arbetsmiljöperspektiv.Material & metod: Intervjuerna genomfördes på tandtekniska laboratorier i Malmö genom att ha semistrukturerade intervjuer. Fem dental laboratorier medverkade i undersökningen, var vid respektive laboratoriechefer och skyddsombud intervjuades.Resultat:Studien påvisade medvetna laboratoriechefer/skyddsombud som hade planer på hur de skulle gå tillväga för att uppnå ett bra resultat med gynnsamma arbetsmiljöförhållande. Fyra av fem respondenter konstaterade att arbetstagarna är medvetna om riskerna som kan uppstå på arbetsplatsen.Slutsats:Slutsatserna som kan dras med beaktande av studiens begränsningar är att:•Intervjuerna visade att respondenterna är medvetna om hur en optimal verksamhet kan uppnås utifrån ett arbetsmiljöperspektiv. Dock tas inte alltid ställning till det på grund av bland annat brist på resurser.•Studien visade att mätningar på ventilation och utsug utförs regelbundet för att säkerställa att allt är under kontroll.
Introduction: The work environment is an important aspect to consider in order to avoid damage. One way to prevent injuries, diseases and poor ergonomics is by using protective equipment that are provided for such things. Another important part of the dental technician profession is that laboratories must take responsibilities for their actions. They need to consider the right use of materials and how to handle them correctly. The laboratory must also focus on the employee’s safety. At last, in order to achieve an optimal result, it is required that laboratories are quality assured.Aim: The aim of this study is to investigate how a dental laboratory looks from a work environment perspectiveMaterial and method: The semi-structed interviews were held in dental laboratories in Malmö. Five dental laboratories participated in the survey in which laboratory manager/safety representative were interviewed.Results: The study showed positive results and awareness among laboratory managers/safety representatives that have knowledge of how to achieve favorable working conditions. Four out of five respondents established that the employees are aware of the risks that may occur in the workplace.Conclusion: Within the scope of the study, the following conclusions can be drawn:• The interviews showed that the respondents are aware of how to achieve an optimal work environment, but they do not always have the resources to do so. • The study showed that measurements of ventilation and suction are performed regularly to ensure that everything is under control.

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Iracheta-Vellve, Arvin. « Innate Immunity As Mediator of Cell Death and Inflammation in Alcoholic Liver Disease ». eScholarship@UMMS, 2017. https://escholarship.umassmed.edu/gsbs_diss/960.

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Central driving forces in the pathogenesis of liver disease are hepatocyte death and immune cell-driven inflammation. The interplay between outcomes, stemming from these two major cell types, is present from the earliest ethanol exposure, and are both determinants in advanced stages of liver disease particularly in alcoholic liver disease (ALD). The complexities associated with advanced ALD are many and therapies are limited. Due to the liver’s role in ethanol metabolism and filtering gut-derived products, it is becoming increasingly clear that innate immunity plays a central role in triggering activation of cell death and inflammatory pathways in ALD. We identified interferon regulatory factor 3 (IRF3) activation as a mediator of hepatocyte death as the first event after ethanol exposure, and the inflammasome as a protein complex responsible for the subsequent inflammatory cascade, driven by the NLRP3 inflammasome. Our novel findings in murine samples and human patients with alcoholic hepatitis demonstrate that ethanol-induced inflammasome activity results in Caspase-1-mediated pyroptosis and extracellular ASC aggregates in the liver and circulation. Pyroptosis can be abrogated by therapeutic inhibition of inflammasome components, NLRP3 or Caspase-1. Taken together, the event leading to mtDNA release into the cytoplasm is the inception of the pathogenesis of ALD, triggering hepatocyte death, culminating in a pro-inflammatory cascade driven by the NLRP3 inflammasome and pyroptotic release of ASC.

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Vachtsevanos, Athanasios. « Probing the molecular basis of melanopsin induced light sensitivity ». Thesis, University of Oxford, 2012. http://ora.ox.ac.uk/objects/uuid:631deeeb-90c0-4e90-b24e-f03e1b318d8b.

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It has been demonstrated that retinal photoreception among mammals extends beyond rods and cones to include a small number of intrinsically photosensitive retinal ganglion cells (pRGCs), which are capable of responding to light due to expression of the melanopsin (OPN4) photopigment. OPN4 may have therapeutic potential if ectopically expressed in the degenerate retina in cases where photoreceptors are lost, but the other molecules involved in this light induced transduction cascade are less well characterized. Therefore I sought to probe further the mechanism of OPN4 mediated light sensitivity by siRNA mediated knock down of specific molecules in two mice models in which complete loss of rods and cones renders them almost exclusively dependent on the OPN4 pathway for light sensitivity. I generated siRNA probes against the long transcript variant of murine Opn4 mRNA and first tested these probes on the murine Neuro2A (N2a) cell line, before assessing effects in C3H/HeN rd and rodless/coneless rd/rd cl mice. siRNA was injected intravitreally into one eye and pupillometry was assessed, combined with molecular analyses at various timepoints. Reverse transcription polymerase chain reaction (RT-PCR) analysis in N2a cells confirmed Opn4 knockdown and immunolabelling techniques identified >85% silencing with siRNA. Pupil responses in the rd and rd/rd cl mice were inhibited by the siRNA injections in vivo which confirmed the functional effect of Opn4 silencing detected by molecular analysis. I therefore present a novel reproducible in vivo model in which siRNA induced silencing of the melanopsin pathway can be assessed by pupillometry and compared to levels of mRNA and protein at specific timepoints. Probes against other putative candidate genes, such as TRPC3, may unravel the molecular interactions of this pathway. This may help in future to induce light sensitivity in other retinal neurons in patients who are completely blind from photoreceptor loss.

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Schilling, Thomas. « The Immune Response to One-Lung Ventilation Clinical and Experimental Studies / ». Doctoral thesis, Uppsala : Acta Universitatis Upsaliensis : Univ.-bibl. [distributör], 2009. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-108851.

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Thèses sur le sujet « Medical laboratory science »

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