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Littérature scientifique sur le sujet « MiRNA-screening »

Auteur : Grafiati

Publié le 1 avril 2023

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Articles de revues sur le sujet "MiRNA-screening"

Ying, Huanchun, Jing Lyu, Tianshu Ying, Jun Li, Shanshan Jin, Jingru Shao, Lili Wang et Hongying Xu. « Risk miRNA screening of ovarian cancer based on miRNA functional synergistic network ». Journal of Ovarian Research 7, n^o 1 (2014) : 9. http://dx.doi.org/10.1186/1757-2215-7-9.

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Nielsen, Søren Jensby, Hanni Willenbrock, Jacob Fog, Jan Stenvang, Thorarinn Blondal, Torben Orntoft, Nils Brünner, Claus Lindbjerg Andersen, Hans J. Nielsen et Adam Baker. « Validation of a plasma-based miRNA PCR test for early detection of colorectal cancer. » Journal of Clinical Oncology 30, n^o 4_suppl (1 février 2012) : 424. http://dx.doi.org/10.1200/jco.2012.30.4_suppl.424.

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424 Background: Colorectal cancer (CRC) is a major cause of mortality in the western world. Early detection of CRC improves survival and screening for CRC has been clinically proven to lower CRC-related mortality in the screening population. However, although population screening programs have been implemented in a number of countries, screening rates among the 50-75 year olds are unsatisfactory. There is therefore a clear unmet need for a quick, sensitive, specific, and minimally invasive screening assay to select at risk individuals for definitive diagnosis by colonoscopy. Methods: In order to detect microRNA (miRNA) biomarkers for CRC in blood plasma, we developed an LNA-enhanced miRNA RT-qPCR platform with high sensitivity and linearity for optimal quantitation of miRNAs from limited plasma samples. A clinical reference- lab compatible workflow that allows for the entire procedure from sample preparation through data acquisition and QC to test result to be completed within one working day was established. A reference melting curve database has been implemented to ensure the integrity of each data point, and appropriate controls monitor plate-to-plate and day-to-day variation. State-of-the-art normalization protocols have been evaluated to ensure optimal normalization of datasets prior to data analysis. Results: We previously determined a miRNA signature in a multi hospital discovery cohort that is differentially expressed between healthy individuals and stage II CRC patients. Here we report on the validation of this miRNA signature in an independent set of plasma samples from CRC patients and healthy volunteers. We have counter-screened the miRNA signature in a set of patients with other prevalent diseases, including hypertension, diabetes, diverticulitis, and others. Conclusions: A plasma miRNA signature for early detection of CRC from patient plasma was developed and validated in an independent clinical sample set. The signature was specific with respect to other diseases prevalent in the screening population. A second large-scale validation project is on-going. We conclude that plasma miRNA biomarkers can constitute an effective minimally invasive approach to population-wide CRC screening.

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Eulalio, Ana, et Miguel Mano. « MicroRNA Screening and the Quest for Biologically Relevant Targets ». Journal of Biomolecular Screening 20, n^o 8 (30 mars 2015) : 1003–17. http://dx.doi.org/10.1177/1087057115578837.

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MicroRNAs (miRNAs) are a class of genome-encoded small RNAs that post-transcriptionally regulate gene expression by repressing target transcripts containing partially or fully complementary binding sites. Despite their relatively low number, miRNAs have been shown to directly regulate a large fraction of the transcriptome. In agreement with their pervasive role in the regulation of eukaryotic gene expression, miRNAs have been implicated in virtually all biological processes, including different pathologies. The use of screening technologies to systematically analyze miRNA function in cell-based assays offers a unique opportunity to gain new insights into complex biological and disease-relevant processes. Given the low complexity of the miRNome and the similarities to small interfering RNA (siRNA) screening experimental approaches, phenotypic screening using genome-wide libraries of miRNA mimics or inhibitors is not, per se, technically challenging. The identification of miRNA targets and, more importantly, the characterization of their mechanisms of action through the identification of the key targets underlying observed phenotypes remain the major challenges of this approach. This article provides an overview of cell-based screenings for miRNA function that were performed in different biological contexts. The advantages and limitations of computational and experimental approaches commonly used to identify miRNA targets are also discussed.

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Roa, W., L. J. Xing, J. Amanie, A. Fairchild, Z. Gabos, T. Nijjar, R. Scrimger et D. Yee. « 14 SCREENING LUNG CANCER WITH MIRNA EXPRESSION PROFILES ». Radiotherapy and Oncology 92 (septembre 2009) : S5. http://dx.doi.org/10.1016/s0167-8140(12)72401-5.

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Oka, Hiroyuki, Koichi Masuno, Takeki Uehara, Toru Okamoto, Yoshiharu Matsuura, Toru Nakano et Shinpei Yamaguchi. « Novel miRNA biomarkers for genotoxicity screening in mouse ». Toxicology 404-405 (juillet 2018) : 68–75. http://dx.doi.org/10.1016/j.tox.2018.05.009.

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Siebert, Marina, Wendy Westbroek, Yu-Chi Chen, Nima Moaven, Yan Li, Maria Luiza Saraiva-Pereira, Scott Martin et Ellen Sidransky. « MiRNAs and glucocerebrosidase : lessons from miRNA mimic screening ». Molecular Genetics and Metabolism 111, n^o 2 (février 2014) : S98. http://dx.doi.org/10.1016/j.ymgme.2013.12.239.

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Zanutto, Susanna, Chiara Maura Ciniselli, Antonino Belfiore, Mara Lecchi, Enzo Masci, Gabriele Delconte, Massimo Primignani et al. « Plasma miRNA‐based signatures in CRC screening programs ». International Journal of Cancer 146, n^o 4 (5 août 2019) : 1164–73. http://dx.doi.org/10.1002/ijc.32573.

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Lorenz, Daniel A., Steve Vander Roest, Martha J. Larsen et Amanda L. Garner. « Development and Implementation of an HTS-Compatible Assay for the Discovery of Selective Small-Molecule Ligands for Pre-microRNAs ». SLAS DISCOVERY : Advancing the Science of Drug Discovery 23, n^o 1 (7 juillet 2017) : 47–54. http://dx.doi.org/10.1177/2472555217717944.

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microRNAs (miRNAs) are small gene regulatory RNAs, and their expression has been found to be dysregulated in a number of human diseases. To facilitate the discovery of small molecules capable of selectively modulating the activity of a specific miRNA, we have utilized new high-throughput screening technology targeting Dicer-mediated pre-miRNA maturation. Pilot screening of ~50,000 small molecules and ~33,000 natural product extract libraries against pre-miR-21 processing indicated the potential of our assay for this goal, yielding a campaign Z′ factor of 0.52 and an average plate signal-to-background (S/B) ratio of 13. Using two-dimensional screening against a second pre-miRNA, pre-let-7d, we evaluated the selectivity of confirmed hits. The results presented demonstrate how high-throughput screening can be used to identify selective small molecules for a target RNA.

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Mellis, David, et Andrea Caporali. « MicroRNA-based therapeutics in cardiovascular disease : screening and delivery to the target ». Biochemical Society Transactions 46, n^o 1 (1 décembre 2017) : 11–21. http://dx.doi.org/10.1042/bst20170037.

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MicroRNAs (miRNAs) are small non-coding RNAs of ∼22 nucleotides, which have increasingly been recognized as potent post-transcriptional regulators of gene expression. MiRNA targeting is defined by the complementarities between positions 2–8 of miRNA 5′-end with generally the 3′-untranslated region of target mRNAs (messenger RNAs). The capacity of miRNAs to simultaneously inhibit many different mRNAs allows for an amplification of biological responses. Hence, miRNAs are extremely attractive targets for therapeutic regulation in several diseases, including cardiovascular. Novel approaches are emerging to identify the miRNA functions in cardiovascular biology processes and to improve miRNA delivery in the heart and vasculature. In the present study, we provide an overview of current studies of miRNA functions in cardiovascular cells by the use of high-content screening. We also discuss the challenge to achieve a safe and targeted delivery of miRNA therapeutics in cardiovascular cells.

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Pfaff, Nils, Jan Fiedler, Angelika Holzmann, Axel Schambach, Thomas Moritz, Tobias Cantz et Thomas Thum. « miRNA screening reveals a new miRNA family stimulating iPS cell generation via regulation of Meox2 ». EMBO reports 12, n^o 11 (23 septembre 2011) : 1153–59. http://dx.doi.org/10.1038/embor.2011.176.

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Thèses sur le sujet "MiRNA-screening"

Bhajun, Ricky. « Une approche réseau pour l’inférence du rôle des microARN dans la corégulation des processus biologiques ». Thesis, Université Grenoble Alpes (ComUE), 2015. http://www.theses.fr/2015GREAS045/document.

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L'interférence par l'ARN est un processus selon lequel un petit ARN non codant se lie à un ARN messager cible dans la cellule pour moduler son expression. Ce mécanisme a été conservé au cours de l'évolution : il est retrouvé aussi bien chez les animaux que chez les végétaux. Nous savons aujourd'hui que le rôle de l'interférence par l'ARN est fondamental, dans le développement embryonnaire comme dans la progression tumorale. Les microARN (miARN) sont des ARN non codant endogènes dont l'une des particularités est leur capacité à réguler tout un ensemble de gènes par interférence avec les ARN messagers. Il est ainsi prédit qu'un seul miARN serait capable de réguler plusieurs centaines de gènes différents. La thèse a consisté en l'analyse de la corégulation médiée par les miARN grâce à l'inférence de réseau basée sur le partage de gènes cibles. La corégulation est un phénomène où plusieurs miARN différents interviennent sur les mêmes familles de gènes et donc sur les mêmes processus biologiques. Le travail a plus spécifiquement consisté en la mise en place d'un réseau de miARN, en son analyse topologique mais également en son interprétation biologique. Le but final était de proposer de nouvelles hypothèses biologiques à tester afin de mieux comprendre la corégulation des processus biologiques par les miARN. Au travers de ces travaux, deux groupes de miARN ont pu être mis en évidence, dont l'un impliqué dans la régulation de la signalisation par les petites GTPases – hypothèse par la suite validée par plusieurs expériences in vitro. Dans un second temps, une communauté de miARN impliquée dans le maintien de la pluripotence des cellules souches a pu également être mise en évidence. Pour compléter ces analyses, une étude systémique de la topologie des réseaux de miARN a été menée afin de mieux comprendre leur intégration dans les réseaux biologiques et leur rôle dans le devenir cellulaire
RNA interference is a process in which a small non-coding RNA will bind to a specific messenger RNA and regulate its expression. This evolutionary conserved mechanism is found in all superior eukaryotes from plants to mammals. Nowadays, we know that RNA interference is a major regulatory process involved in developmental biology and tumor progression. MicroRNAs (miRNAs) are endogenous (coded in and produced by the cell) non-coding RNAs which are able to regulate a whole set of genes, typically hundreds of genes. This doctoral thesis consisted in the analysis of the miRNA mediated coregulation through a network approach based on target sharing. Coregulation is the process where many different miRNAs will regulate the same set of genes and thus the same biological process. In particular, the work consisted in the inference of a miRNA network, in its topological analysis and also its biological interpretation. Indeed, the final aim of the work was to generate new biological hypothesis. As such, two different groups of miRNAs were first retrieved. One of them was predicted to be involved in the small GTPase signaling and was further validated in vitro. Moreover, a miRNA community involved in the maintenance of stem cells pluripotency was also discovered. Finally, a systemic analysis of the target-based miRNAs network was conducted to better understand their integration with biologic networks and their role in cell fate

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Naidoo, Jerolen. « Functional miRNA-based Phenotypic Screening as a tool to delineate HIV-host interactions and facilitate Novel Drug Discovery ». Thesis, Faculty of Health Sciences, 2021. http://hdl.handle.net/11427/33227.

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Human Immunodeficiency Virus (HIV) is the causative agent of AIDS, a disease which affects over 24 million people globally and for which there is neither curative treatment nor vaccine available. As an intracellular pathogen that encodes only 15 proteins HIV-1 is highly dependent upon its host's cellular machinery in order to complete its life cycle. Host-directed therapy thus represents a potentially lucrative strategy for the development of novel anti-HIV therapies. microRNAs (miRNAs) are short noncoding RNA molecules that function as part of the endogenous RNA interference system which governs post transcriptional gene regulation. Current knowledge has placed miRNAs at the crux of HIV-host interactions, yet the functional relevance of the majority of the human miRNAome with regards to HIV replication has remained unknown. A microscopy-based high content screening (HCS) approach was thus developed to systematically evaluate the significance of augmenting or inhibiting the function of individual host miRNAs on the replication dynamics of HIV. A bespoke image analysis and data mining pipeline recovered 56 host miRNAs associated with suppressed HIV replication and 28 host miRNAs associated with enhanced HIV replication. Notably, the HIV-modulating potential of 80 of these miRNAs was previously unknown. Furthermore, HCS also uncovered a novel role for the miR-200 family in the modulation of HIV replication. In silico miRNA target identification and pathway enrichment analysis identified 24 pathways associated exclusively with suppressed HIV replication, 10 pathways associated exclusively with enhanced HIV replication and 38 functional pathways enriched for both enhanced and suppressed viral replication. These included a number of pathways previously implicated in HIV replication such as the PI3K, MAPK, TNF and WNT signalling pathways but also revealed novel functional associations including that of the Hippo signalling pathway. Intriguingly pathway analysis revealed an enrichment for host factors associated with viral carcinogenesis and a convergence on host processes and functional targets classically associated with chemotherapy including host DNA damage repair, cell cycle and tyrosine kinase receptor-mediated signalling. Experimental validation confirmed that HIV replication induced an aberrant cell survival phenotype in response to chemically induced DNA damage but this effect was reversed when DNA damage was induced prior to HIV exposure. A series of compound-based validation screens were thus undertaken in order to verify the functional associations recovered by miRNA screening. A targeted collection of 293 small molecule inhibitors, including a number of FDA-approved chemotherapeutics, were screened for HIV modulating activity. Novel anti-HIV activity was recovered for over 40 compounds including a number of FDA-approved therapies. Compound-target enrichment analysis revealed a strong concordance with functional associations initially described by miRNA-based HCS including EGFR-mediated signalling and DNA damage repair. Concordant HIV-suppressive activity was also recovered for miRNAs and compounds with common functional targets. The outcomes of this study thus represent a significant and novel contribution to current knowledge on HIV-host interactions. Furthermore, these findings have characterised novel miRNA and small molecule candidates for the treatment of HIV and have successfully demonstrated the utility of miRNA-based HCS for novel-drug discovery and drug repositioning.

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Villegas, Mirón Pablo 1991. « A Comprehensive screening of X-linked and miRNA-driven signatures of positive selection and the role of cis-regulatory elements in the human genome ». Doctoral thesis, TDX (Tesis Doctorals en Xarxa), 2021. http://hdl.handle.net/10803/672657.

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The evolutionary history of the human genome has been shaped by the selection of multiple elements in response to different environmental pressures. The analysis of regulatory adaptations and the particularities of sexual chromosomes are key to understanding the different evolutionary outcomes of some of these processes. In this thesis, we describe how recent selection has shaped the X chromosome in human populations, focusing on several selection candidates, the special X-linked inheritance properties and the role of regulatory elements. We also propose the relevant implication of human enhancers in tissue-specific regulatory programs. The expression of genes implicated in tissue-specific functions is seen to be regulated mainly by enhancers located in introns, while ubiquitously expressed housekeeping genes are predominantly controlled by intergenic enhancers. The evolutionary role of human miRNAs are also analysed with special emphasis on their global patterns of diversity and their implication in population-specific prevalence in some of the most common human disorders
La historia evolutiva del genoma humano ha sido configurada por la selección de numerosos elementos en respuesta a distintas presiones evolutivas. El análisis de adaptaciones regulatorias y de las particularidades de los cromosomas sexuales son clave para entender las distintas consecuencias de algunos de estos procesos. En esta tesis describimos cómo procesos de selección reciente han configurado el cromosoma X en distintas poblaciones humanas, centrándonos en varios candidatos bajo selección, sus propiedades hereditarias y el papel de elementos regulatorios. También describimos la relevancia de enhancers humanos en los programas regulatorios de tejidos específicos. La expresión de genes implicados en las funciones específicas de tejido aparece principalmente regulada por enhancers ubicados en intrones, por otro lado, genes implicados en el mantenimiento básico de la célula están predominantemente controlados por enhancers intergénicos. También analizamos el papel evolutivo de microRNAs humanos, con especial énfasis en patrones de diversidad globales y su implicación en prevalencias poblaciones de algunas de las enfermedades humanas más comunes.

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Mirihana, Arachchilage Gayan S. « REGULATORY ROLES OF G-QUADRUPLEX IN microRNA PROCESSING AND mRNA TRANSLATION ». Kent State University / OhioLINK, 2016. http://rave.ohiolink.edu/etdc/view?acc_num=kent1469576783.

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Dojahn, Claudine. « Synthese und Screening von Inhibitoren der mikroRNA-Reifung ». Doctoral thesis, Humboldt-Universität zu Berlin, Mathematisch-Naturwissenschaftliche Fakultät I, 2013. http://dx.doi.org/10.18452/16748.

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Das Ziel dieser Arbeit war die Synthese niedermolekularer Verbindungen, die an die prä-miRNA binden und dadurch die Reifung zur miRNA inhibieren. Daher sollten die RNA-Binder 2-Desoxystreptamin sowie Neamin mit Alkinen funktionalisiert und durch Kupfer-katalysierte Azid-Alkin 1,3-dipolare Cycloaddition (CuAAC) mit verschiedenen bivalenten Aziden verknüpft werden. Im Rahmen dieses Projekts wurde der synthetische Zugang zu den benötigten Alkin- sowie Azid-funktionalisierten Grundbausteinen optimiert. Ferner wurde ein effektives und zuverlässiges Protokoll für die CuAAC erarbeitet, welches es ermöglichte, 88 Testsubstanzen in guter Ausbeute und hoher Reinheit zu isolieren. Anschließend wurde die Substanzbibliothek in einem BRCA-Reifungsassay unter kompetitiven Bedingungen auf die Inhibition der miRNA-Reifung getestet. Dabei wurden mehrere potente Inhibitoren der miRNA-Reifung mit IC50-Werten von bis zu 0.5 µM identifiziert. Der zweite Schwerpunkt dieser Arbeit lag auf der Etablierung einer chemo-enzymatische RNA-Funktionalisierungsstrategie um prä-miRNA-Sonden herzustellen: In-vitro-Transkriptionen mit der T7-RNA-Polymerase sowie Ligationen mit der T4 RNA Ligase 1 waren das Fundament der enzymatischen RNA-Synthese, während die Funktionalisierung der RNA durch CuAAC erzielt wurde. Dafür wurden die neuen Verbindungen O-(5‘-Guanosin)-O-propargylmonophosphat sowie 3‘,5‘-O,O-Bisphosphat-5-ethinyluridin synthetisiert, durch in-vitro-Tran¬skription an das 5‘-Ende von prä-miRNAs eingeführt und anschließend durch CuAAC mit einem Fluoreszenzlöscher modifiziert. Das Uridinbisphosphat wurde durch CuAAC mit einem Fluorophor markiert und anschließend effizient mit der T4 RNA Ligase 1 an das 3‘-Ende verschiedener prä-miRNAs ligiert. Darüber hinaus war es auch möglich, das Alkin-modifizierte Uridinbisphosphat an das 3‘-Ende von prä-miRNAs zu ligieren, dieses durch eine zweite Ligation an eine definierte interne Position zu verschieben und abschließend durch CuAAC zu funktionalisieren.
The objective of this work was the synthesis of small molecules, which bind to pre-miRNAs to prevent their maturation to fully active miRNAs. To create a substance library of bivalent inhibitors, the RNA binding motifs 2-deoxystreptamine as well as neamine were alkyne modified and linked with several bisazides via a copper catalyzed alkyne-azide cycloaddition (CuAAC). Hence, optimized syntheses of the basic building blocks along with an effective and reliable CuAAC-protocol were established. 88 test substances were isolated in good yield and high purity. Finally they were analyzed with regard to their potential to selectively inhibit the miRNA maturation. For this purpose, the assay was performed under competitive conditions with a set of two pre-miRNA-pairs. The initial screening revealed several inhibitors with IC50-values in the lower µM range. The second focus of this work was on the development of a synthetic access to alkyne modified ribonucleotides to establish a chemo-enzymatic functionalization strategy for RNAs using in-vitro-transcription, ligation and CuAAC. In this context, the syntheses of 3‘,5‘-O,O-bisphosphate-5-ethinyl uridine and O-(5‘-guanosine)-O-propargyl monophos-phate are described for the first time. The guanosine monophosphate was used as transcription starter to address the 5’-end of RNA and was consecutively labeled with an azido-tagged quencher. The uridine bisphosphate was conjugated with a fluorophor and introduced to the 3’-end of RNAs by T4 RNA ligase 1. Moreover, the uridine bisphosphate can be ligated to the 3’-end without a fluorophor attached, to serve as a connecting point for a further ligation with an oligonucleotide of any length. Thereby, the former terminal alkynylated uridine was shifted to a defined internal position by successive enzymatic reactions and was successfully derivatized with a fluorophor by CuAAC.

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Chapitres de livres sur le sujet "MiRNA-screening"

Han, Bo. « Screening miRNA for Functional Significance by 3D Cell Culture System ». Dans MicroRNA Protocols, 193–201. New York, NY : Springer New York, 2018. http://dx.doi.org/10.1007/978-1-4939-7601-0_16.

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Armstrong, Richard N., Hilary A. A. Colyer et Ken I. Mills. « Screening for miRNA Expression Changes Using Quantitative PCR (Q-PCR) ». Dans Methods in Molecular Biology, 293–302. Totowa, NJ : Humana Press, 2012. http://dx.doi.org/10.1007/978-1-61779-612-8_18.

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Armstrong, Richard N., Hilary A. A. Colyer et Ken I. Mills. « Screening for miRNA Expression Changes Using Quantitative PCR (Q-PCR) ». Dans Methods in Molecular Biology, E1. Totowa, NJ : Humana Press, 2012. http://dx.doi.org/10.1007/978-1-61779-612-8_29.

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Guijarro, Maria V., et Amancio Carnero. « Genome-Wide miRNA Screening for Genes Bypassing Oncogene-Induced Senescence ». Dans Methods in Molecular Biology, 53–68. New York, NY : Springer New York, 2016. http://dx.doi.org/10.1007/978-1-4939-6670-7_5.

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Li, Yan, Bing Chen et Shenglin Huang. « Identification of circRNAs for miRNA Targets by Argonaute2 RNA Immunoprecipitation and Luciferase Screening Assays ». Dans Methods in Molecular Biology, 209–18. New York, NY : Springer New York, 2018. http://dx.doi.org/10.1007/978-1-4939-7562-4_17.

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Haga, Christopher L., Sai Pradeep Velagapudi, Jessica L. Childs-Disney, Jacqueline Strivelli, Matthew D. Disney et Donald G. Phinney. « Rapid Generation of miRNA Inhibitor Leads by Bioinformatics and Efficient High-Throughput Screening Methods ». Dans Methods in Molecular Biology, 179–98. New York, NY : Springer New York, 2016. http://dx.doi.org/10.1007/978-1-4939-6563-2_13.

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Periwal, Vinita, et Vinod Scaria. « Machine Learning Approaches Toward Building Predictive Models for Small Molecule Modulators of miRNA and Its Utility in Virtual Screening of Molecular Databases ». Dans Methods in Molecular Biology, 155–68. New York, NY : Springer New York, 2016. http://dx.doi.org/10.1007/978-1-4939-6563-2_11.

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« In Silico Screening of miRNA Target Sites ». Dans Encyclopedia of Systems Biology, 1015. New York, NY : Springer New York, 2013. http://dx.doi.org/10.1007/978-1-4419-9863-7_100674.

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Power, Michael L., Caroline W. Quaglieri, Eda G. Reed et Jay Schulkin. « The Functions of MicroRNA in Female Reproduction ». Dans Integrating Evolutionary Biology into Medical Education, 132–47. Oxford University Press, 2019. http://dx.doi.org/10.1093/oso/9780198814153.003.0008.

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MicroRNA (miRNA) are small RNA molecules of about 20–25 nucleotides in length which act to regulate gene expression post-transcription primarily by blocking the translation of messenger ribonucleic acid (mRNA). miRNA play important roles in the pathology of cancer, and also during normal pregnancy and lactation. The study of miRNAs related to cancer screening and treatment is perhaps the most developed of the clinical applications regarding miRNA, particularly for breast cancer. Evidence miRNAs affect pregnancy is strong, but the specifics remain poorly understood as the relationship between mother and neonate is complex. Within this relationship, miRNA in milk remain the most unknown, but it is hypothesized that milk miRNA play a role in regulation of both the mammary gland and in the neonate. This chapter describes ways biomedical researchers would gain from viewing miRNAs from an evolutionary perspective, specifically for research involving potential therapeutic interventions for women.

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Jayasinghe, Ravindri, Umesh Jayarajah et Sanjeewa Seneviratne. « Circulating Biomarkers in Predicting Pathological Response to Neoadjuvant Therapy for Colorectal Cancer ». Dans Biomarkers in Medicine, 113–32. BENTHAM SCIENCE PUBLISHERS, 2022. http://dx.doi.org/10.2174/9789815040463122010008.

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Circulating biomarkers show promise in the management of many cancers.They have become the novel non-invasive approach to complement the currentstrategies in colorectal cancer (CRC) management. Their ability in guiding diagnosis,evaluating response to treatment, screening and prognosis is phenomenal, especiallywhen it comes to their minimally invasive nature. These “liquid biopsies,” which showpotential for replacing invasive surgical biopsies, provide useful information on theprimary and metastatic disease by providing an insight into cancer biology. Analysis ofblood and body fluids for circulating tumour DNA (ctDNA), carcinoembryonic antigen(CEA), circulating tumour cells (CTC), or circulating micro RNA (miRNA) showspotential for improving CRC management. Recognizing a predictive model to assessresponse to neoadjuvant chemotherapy would help in better patient selection. Thisreview was conducted with the aim of outlining the use of circulatory biomarkers incurrent practice and their effectiveness in the management of patients having CRC witha focus on response to neoadjuvant therapy.

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Actes de conférences sur le sujet "MiRNA-screening"

Koga, Yoshikatsu, Nobuyoshi Yamazaki, Yasuo Kakugawa, Takahisa Matsuda, Masaaki Ito, Yutaka Saito, Hiroshi Saito et Yasuhiro Matsumura. « Abstract 5292 : Multitarget fecal miRNA test combined with fecal occult blood test and fecal miRNA test for colorectal cancer screening ». Dans Proceedings : AACR Annual Meeting 2017 ; April 1-5, 2017 ; Washington, DC. American Association for Cancer Research, 2017. http://dx.doi.org/10.1158/1538-7445.am2017-5292.

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Koga, Yoshikatsu, Masahiro Yasunaga et Yasuhiro Matsumura. « Abstract 4448 : Fecal miRNA test using microRNA expressions of exfoliated colonocytes for colorectalcancer screening ». Dans Proceedings : AACR 103rd Annual Meeting 2012‐‐ Mar 31‐Apr 4, 2012 ; Chicago, IL. American Association for Cancer Research, 2012. http://dx.doi.org/10.1158/1538-7445.am2012-4448.

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Heneghan, HM, N. Miller, N. Healy, J. Newell et MJ Kerin. « Abstract P3-10-02 : Circulating miRNA Signature : Potential Screening and Prognostic Tool for Breast Cancer ». Dans Abstracts : Thirty-Third Annual CTRC‐AACR San Antonio Breast Cancer Symposium‐‐ Dec 8‐12, 2010 ; San Antonio, TX. American Association for Cancer Research, 2010. http://dx.doi.org/10.1158/0008-5472.sabcs10-p3-10-02.

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Michailidi, Christina, Tal Hadar, Kaitlyn Zenner, Mark Schoenberg, George Netto, David Sidransky et Mohammad O. Hoque. « Abstract 4193 : Exposure to arsenic and miRNA deregulation : a potential non-invasive screening tool for urothelial cell carcinoma. » Dans Proceedings : AACR 104th Annual Meeting 2013 ; Apr 6-10, 2013 ; Washington, DC. American Association for Cancer Research, 2013. http://dx.doi.org/10.1158/1538-7445.am2013-4193.

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Naccarati, Alessio, Daniela De Maria, Francesca Cordero, Barbara Pardini, Stavrula Gouzounis, Maddalena Arigoni, Raffaella Rizzolo et al. « Abstract LB-366 : miRNA as markers of CIN risk and persistence for optimization of HPV-based cervical cancer screening ». Dans Proceedings : AACR 107th Annual Meeting 2016 ; April 16-20, 2016 ; New Orleans, LA. American Association for Cancer Research, 2016. http://dx.doi.org/10.1158/1538-7445.am2016-lb-366.

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Deng, Jianzhi, Yuehan Zhou, Xiaohui Cheng et Mingjun Xu. « Screening and Analysis of lncRNA Biomarkers and the Relationship with the Corresponding Regulatory miRNA for Progressive Acute Lymphoblastic Leukemia ». Dans Proceedings of the 2019 International Conference on Modeling, Simulation and Big Data Analysis (MSBDA 2019). Paris, France : Atlantis Press, 2019. http://dx.doi.org/10.2991/msbda-19.2019.51.

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Boeri, Mattia, Carla Verri, Cristina Borzi, Todd Holscher, Matteo Dugo, Andrea Devecchi, Elisa Romeo et al. « Abstract 2753 : Mutational profiles from targeted NGS combine with miRNA-based liquid biopsy to predict survival in LDCT screening-detected lung cancers ». Dans Proceedings : AACR Annual Meeting 2017 ; April 1-5, 2017 ; Washington, DC. American Association for Cancer Research, 2017. http://dx.doi.org/10.1158/1538-7445.am2017-2753.

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Driscoll, James J., et Sajjeev Jagannathan. « Abstract 1462 : Tandem genome-wide and functional screening reveals that MiRNA-29 regulates the proteasome activator PSME4 to promote therapeutic resistance in myeloma ». Dans Proceedings : AACR Annual Meeting 2014 ; April 5-9, 2014 ; San Diego, CA. American Association for Cancer Research, 2014. http://dx.doi.org/10.1158/1538-7445.am2014-1462.

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Östling, Päivi, Suvi-Katri Leivonen, Pekka Kohonen, Anna Aakula, Petteri Hintsanen, Tero Aittokallio, Merja Perälä et Olli Kallioniemi. « Abstract 5273 : Systematic functional screening, miRNA and target expression analyses reveal miR-19, -124, -583, -129 and -876 as key nodes in prostate cancer proliferation networks. » Dans Proceedings : AACR 104th Annual Meeting 2013 ; Apr 6-10, 2013 ; Washington, DC. American Association for Cancer Research, 2013. http://dx.doi.org/10.1158/1538-7445.am2013-5273.

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Rapports d'organisations sur le sujet "MiRNA-screening"

Whitham, Steven A., Amit Gal-On et Victor Gaba. Post-transcriptional Regulation of Host Genes Involved with Symptom Expression in Potyviral Infections. United States Department of Agriculture, juin 2012. http://dx.doi.org/10.32747/2012.7593391.bard.

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Understanding how RNA viruses cause disease symptoms in their hosts is expected to provide information that can be exploited to enhance modern agriculture. The helper component-proteinase (HC-Pro) protein of potyviruses has been implicated in symptom development. Previously, we demonstrated that symptom expression is associated with binding of duplex small-interfering-RNA (duplex-siRNA) to a highly conserved FRNK amino acid motif in the HC-Pro of Zucchini yellow mosaic virus (ZYMV). This binding activity also alters host microRNA (miRNA) profiles. In Turnip mosaic virus (TuMV), which infects the model plant Arabidopsis, mutation of the FRNK motif to FINK was lethal providing further indication of the importance of this motif to HC-Pro function. In this continuation project, our goal was to further investigate how ZYMV and TuMV cause the mis-expression of genes in cucurbits and Arabidopsis, respectively, and to correlate altered gene expression with disease symptoms. Objective 1 was to examine the roles of aromatic and positively charged residues F164RNH and K215RLF adjacent to FR180NK in small RNA binding. Objective 2 was to determine the target genes of the miRNAs which change during HC-Pro expression in infected tissues and transgenic cucumber. Objective 3 was to characterize RNA silencing mechanisms underlying differential expression of host genes. Objective 4 was to analyze the function of miRNA target genes and differentially expressed genes in potyvirus-infected tissues. We found that the charged K/R amino acid residues in the FKNH and KRLF motifs are essential for virus viability. Replacement of K to I in FKNH disrupted duplex-siRNA binding and virus infectivity, while in KRLF mutants duplex-siRNA binding was maintained and virus infectivity was limited: symptomless following a recovery phenomenon. These findings expanded the duplex-siRNA binding activity of HC-Pro to include the adjacent FRNK and FRNH sites. ZYMV causes many squash miRNAs to hyper-accumulate such as miR166, miR390, mir168, and many others. Screening of mir target genes showed that only INCURVATA-4 and PHAVOLUTA were significantly upregulated following ZYMVFRNK infection. Supporting this finding, we found similar developmental symptoms in transgenic Arabidopsis overexpressing P1-HC-Pro of a range of potyviruses to those observed in miR166 mutants. We characterized increased transcription of AGO1 in response to infection with both ZYMV strains. Differences in viral siRNA profiles and accumulation between mild and severe virus infections were characterized by Illumina sequencing, probably due to the differences in HC-Pro binding activity. We determined that the TuMV FINK mutant could accumulate and cause symptoms in dcl2 dcl4 or dcl2 dcl3 dcl4 mutants similar to TuMV FRNK in wild type Arabidopsis plants. These dcl mutant plants are defective in antiviral defenses, and the results show that factors other than HC-ProFRNK motif can induce symptoms in virus-infected plants. As a result of this work, we have a better understanding of the FRNK and FKNH amino acid motifs of HC-Pro and their contributions to the duplex-siRNA binding functions. We have identified plant genes that potentially contribute to infectivity and symptoms of virus infected plants when they are mis-expressed during potyviral infections. The results establish that there are multiple underlying molecular mechanisms that lead viral pathogenicity, some dependent on HC-Pro. The potential benefits include the development of novel strategies for controlling diseases caused by viruses, methods to ensure stable expression of transgenes in genetically improved crops, and improved potyvirus vectors for expression of proteins or peptides in plants.

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