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Thèses sur le sujet « Molecular cloning »

Auteur : Grafiati

Publié le 4 juin 2021

Mis à jour le 25 juillet 2025

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1

Bates, Nancy Carol. "Characterization of cbg : a cloned gene encoding an extracellular [beta]-glucosidase from Cellulomonas fimi." Thesis, University of British Columbia, 1987. http://hdl.handle.net/2429/26163.

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A group of Escherichia coli clones harbouring recombinant pBR322 plasmid, containing Cellulomonas fimi DNA inserts, that reacted with antiserum to C.fimi culture supernatant, was screened for their ability to hydrolyze carboxymethyl cellulose (CMC) and 4-methylumbeliferyll-β-D-cellobioside (MUC). A clone, pEC62, hydrolyzed MUC but did not hydrolyze CMC. The recombinant enzyme encoded by pEC62 was shown to be a β-glucosidase (cellobiase). C.fimii itself was shown to encode an extracellular β-glucosidase in C.fimi. This is the first report of an extracellular β-glucosidase from a bacterium. Del

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2

Seto, Nina Oi Ling. "The copper-zinc superoxide dismutase gene from Drosophila melanogaster : attempts to clone the gene using two mixed sequence oligonucleotide probes." Thesis, University of British Columbia, 1987. http://hdl.handle.net/2429/26534.

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Superoxide dismutase is an enzyme which scavenges superoxide radicals and is thought to be a longevity determinant, as there exists a positive correlation between superoxide dismutase concentration and maximum life span potential. The cytosolic CuZn superoxide dismutase in D. melanogaster has been purified and sequenced, but the gene has not been cloned. However, when it is available the CuZn SOD gene may be reintroduced into the Drosophila genome via the P-element transformation system so its effects on the life span potential of Drosophila may be studied. This study describes attempts to clo

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3

Woodruff, Wendy Anne. "Cloning and characterization of the oprF gene for protein F from Pseudomonas aeruginosa." Thesis, University of British Columbia, 1988. http://hdl.handle.net/2429/29218.

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The oprF gene encoding porin protein F from Pseudomonas aeruginosa was cloned onto a cosmid vector into Escherichia coli. Protein F was expressed in large amounts in E. coli and retained its heat- and reduction-modifiable and immunological characteristics. The cloned oprF gene product was purified from E. coli and characterized with respect to pore-forming ability in black lipid bilayers. Small channels, with an average single channel conductance of approximately 0.4 nS, were observed. A similar small channel size was observed for native protein F. The oprF sequences were used as a DNA-DNA hyb

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4

Boyes, Barry Edward. "Molecular cloning of the human Substantia innominata : characterization of a brain large mRNA." Thesis, University of British Columbia, 1990. http://hdl.handle.net/2429/30960.

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Brain tissue samples were collected from individuals with histologically and biochemically confirmed Alzheimer's Disease (AD), as well as from a group of individuals without any signs of neurological disease (NNC). Ribonucleic acid (RNA) was extracted from these tissues, characterized by several chemical methods, and the yields were compared between AD and NNC groups. High molecular weight RNA could be effectively extracted from frozen postmortem human brain. In comparison to the NNC group, tissue RNA levels were reduced in the AD hippocampus, but not in the temporal cortex or substantia innom

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5

Lush, Michael John. "Molecular cloning of neuropathy target esterase." Thesis, University of Leicester, 1998. http://hdl.handle.net/2381/29627.

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A single ingestion of certain organophosphorus esters (OPs) can cause a syndrome known as Organophosphate Induced Delayed Polyneuropathy (OPIDP), a paralysing neuropathy with degeneration of long axons, developing after a latent period of approximately one to three weeks. The primary target of these OPs has been shown to be a 155kDa neural protein designated Neuropathy Target Esterase (NTE), and the toxic effects apparently due to the covalent inhibition and subsequent secondary modification of this protein. Recently NTE has been purified to apparent homogeneity using a novel biotinylated OP a

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6

Long, Graham Stanley. "Molecular cloning of bacteriophage K1E endosialidase." Thesis, University of Cambridge, 1994. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.339539.

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7

McNair, Alan Thomas. "Molecular cloning of Fasciola hepatica antigens." Thesis, Queen's University Belfast, 1990. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.335604.

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8

Choi, Wai To. "Molecular cloning of ribosome-inactivating proteins." HKBU Institutional Repository, 1996. http://repository.hkbu.edu.hk/etd_ra/63.

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9

Fisher, Adam B. "ex vivo DNA cloning." VCU Scholars Compass, 2015. http://scholarscompass.vcu.edu/etd/3962.

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Genetic engineering of microbes has developed rapidly along with our ability to synthesize DNA de novo. Yet, even with decreasing DNA synthesis costs there remains a need for inexpensive, rapid and reliable methods for assembling synthetic DNA into larger constructs or combinatorial libraries. While technological advances have resulted in powerful techniques for in vitro and in vivo assembly of DNA, each suffers inherent disadvantages. Here, an ex vivo DNA cloning suite using crude cellular lysates derived from E. coli is demonstrated to amplify and assemble DNA containing small sequence homol

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10

Wakarchuk, Warren William. "The molecular cloning and characterization of a Beta-glucosidase gene from an Agrobacterium." Thesis, University of British Columbia, 1987. http://hdl.handle.net/2429/27559.

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The β-glucosidase (Abg) from ATCC 21400, an Agrobacterium species, was purified to homogeneity. The protein was cleaved with cyanogen bromide and the peptides were purified by reversed phase high pressure liquid chromatography. The partial amino-acid sequences for three CNBr peptides, CNBr1, CNBr2 and CNBr3, were determined by automated Edman degradation. A sequence from CNBr2 was used to synthesize a mixture of oligonucleotides which was used as a hybridization probe to identify a recombinant DNA clone carrying the gene for β-glucosidase. A single clone was isolated which expressed an enzymat

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11

Mulcahy, Anthony Francis. "The molecular cloning and characterisation of autoantigens." Thesis, University of Newcastle Upon Tyne, 1998. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.242453.

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12

Paine, Mark John Ingraham. "Molecular cloning of Echis carinatus venom genes." Thesis, University of Liverpool, 1990. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.291952.

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13

Caird, Mandy Ruth. "Molecular cloning studies on DNA polymerase alpha." Thesis, University of Aberdeen, 1989. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.292388.

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The DNA polymerase α assay systems and partial purification techniques already in use in the laboratory were improved by isolating better-activated DNA and freezing cells prior to sonication. The suitability of monoclonal antibodies against <i>Xenopus laevis</i> and human KB cell (Tanaka <i>et al</i>., 1982) DNA polymerase α was established as a prelude to screening expression libraries. All were found to inhibit polymerase activity by approximately 50%. Two out of three anti-<i>Xenopus laevis</i> DNA polymerase α antibodies and three out of four anti-human DNA polymerase α antibodies bound an

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14

Mackinnon, Charlotte M. "Molecular cloning of human complement component Cls." Thesis, University of Aberdeen, 1988. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.327928.

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Cls cDNA clones, which together contained the entire coding region of the protein, were isolated from two human-liver cDNA libraries. The initial Cls clones were identified using a synthetic oligonucleotide probe which corresponded to a region of low degeneracy near the C-terminus of the Cls catalytic chain. Fragments of the Cls cDNA were used to screen a cosmid library in an attempt to isolate the Cls gene, but this proved unsuccessful and no positive clones were isolated. The complete primary sequence of Cls revealed that the homology between the Cls and Clr catalytic chains also extends thr

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15

Moser, Bernhard. "Molecular cloning, characterization and expression of the endoglucanase C gene of Cellulomonas fimi and properties of the native and recombinant gene products." Thesis, University of British Columbia, 1988. http://hdl.handle.net/2429/29036.

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In addition to substrate-associated cellulases, Cellulomonas fimi secretes endoglucanases ( endo-1, 4-β-D-glucan glucanohydrolases, EC 3.2.1.4. ) which are recovered from the cellulose-free culture supernatant of cells grown on microcrystalline cellulose. Two such enzymes, C3.1 and C3.2 with Mrs of 130'000 and 120'000, respectively, were purified to homogeneity. The two endoglucanases were shown to share the same N-terminal amino acid sequence and to hydrolyze carboxymethylcellulose ( CMC ) with similar efficiencies ( 236u/mg protein for C3.1 and 367u/mg protein for C3.2 ). The recombinant la

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16

Doucette, Stephanie A. "Cloning of Bovine Placental Lactogen and Production in Vitro." Fogler Library, University of Maine, 2003. http://www.library.umaine.edu/theses/pdf/DoucetteSA2003.pdf.

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17

Lee, Rivera Irene. "Cloning and characterization of mTom40." Thesis, McGill University, 1998. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=21588.

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The majority of mitochondrial proteins are synthesized on cytoplasmic polysomes and subsequently imported to a specific subcompartment within the organelle. In order to translocate cytosolic proteins into mitochondria a protein import system is located in the outer and inner mitochondrial membranes. The genetic composition of this complex has been well characterized in mitochondria of S. cerevisiae and N. crassa. However, little is known about the components of the import machinery in higher eukaryotes. Until today only homologues of Tom20 and Tom17 as well as two new proteins Metaxin and hTom

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18

Suen, Ki-Ling. "Cloning of protein kinase genes from a carrot cDNA library." Diss., Georgia Institute of Technology, 1991. http://hdl.handle.net/1853/25431.

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19

Law, Katherine Mary. "Molecular studies of Theiler's murine encephalomyletis virus." Thesis, University of Cambridge, 1990. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.357801.

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20

Yang, Xiaolong. "Molecular cloning and characterization of human BAG-1." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1999. http://www.collectionscanada.ca/obj/s4/f2/dsk1/tape7/PQDD_0016/NQ47506.pdf.

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21

Tse, Chi-hang. "Molecular cloning of the goldfish dopamine D2 receptor." Click to view the E-thesis via HKUTO, 1998. http://sunzi.lib.hku.hk/hkuto/record/B42128511.

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22

Townsend, Paul Andrew. "The molecular basis of osteoblast adhesion." Thesis, University College London (University of London), 1997. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.263651.

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23

Zhang, Ling 1962. "Molecular cloning and characterization of the chicken ornithine decarboxylase gene." Thesis, McGill University, 1994. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=22831.

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Ornithine decarboxylase (ODC) is the rate determining enzyme in the biosynthesis of polyamines which are essential for cell growth. In chickens, significantly higher bioactivity is reported in broiler than in egg layer strains of chickens (Bulfield et al., 1988). To characterize the genetic differences in growth rates and ODC levels in chickens, an ODC cDNA and genomic gene were cloned and sequenced. Sequencing of ODC cDNA revealed that this clone (pODZ3: 2,052 bp) was not a full length of ODC cDNA and contained 2 putative introns. The open reading frame (introns deleted) coded for a protein o

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24

Freeman, John Douglas. "The cloning of polyhomeotic, a complex Drosophila locus required for segment determination and cuticular differentiation." Thesis, University of British Columbia, 1987. http://hdl.handle.net/2429/26256.

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The polyhomeotic (ph) locus of Drosophila melanogaster has been characterized genetically. Early studies showed that ph is a member of the Polycomb (Pc) group. These genes have similar phenotypes and are required for normal segment determination. Recent analyses of amorphic ph mutations show that the ph locus is complex, has a strong maternal effect and plays a role in cuticular development. To test the function of ph at the molecular level, the cloning of the ph locus was undertaken. One strain had been shown to contain a P element insertion near ph. A genomic library was prepared from this s

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25

Zhu, Tong. "Molecular cloning and characterization of a novel mammalian myosin I." Case Western Reserve University School of Graduate Studies / OhioLINK, 1996. http://rave.ohiolink.edu/etdc/view?acc_num=case1062006565.

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26

Tu, Liwen. "Cloning and sequence analysis of multiple genes from Bifidobacterium infantis /." free to MU campus, to others for purchase, 2004. http://wwwlib.umi.com/cr/mo/fullcit?p3137758.

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27

Huo, Longfei, and 霍龍飛. "Molecular cloning and functional studies of cyprinid calmodulin." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2004. http://hub.hku.hk/bib/B3016316X.

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28

Choi, Bo Yon Linda. "Molecular cloning and characterization of zebrafish connexin 43." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1998. http://www.collectionscanada.ca/obj/s4/f2/dsk2/ftp01/MQ36014.pdf.

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29

Greer, Peter Alan. "Molecular cloning of measles virus nucleic acid sequences." Thesis, McGill University, 1985. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=71952.

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A library of cDNA clones was prepared from measles virus infected cells. Six classes of viral specific cDNA sequences were identified by a combination of Northern and Southern blot hybridization analysis.<br>Clones corresponding to measles virus NP, P/C and M genes were initially identified by hybridization selection translation experiments. Restriction mapping analysis and comparison with the recently published nucleic acid sequences have confirmed the identities of these clones.<br>The cloned sequences corresponding to the NP and M genes were subcloned into a plasmid vector which contains th

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30

謝志恒 and Chi-hang Tse. "Molecular cloning of the goldfish dopamine D2 receptor." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 1998. http://hub.hku.hk/bib/B42128511.

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31

Kwok, Ho-yan Amy, and 郭可茵. "Molecular cloning and characterization of chicken prostaglandin receptors." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2008. http://hub.hku.hk/bib/B41508336.

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32

Martin, Samuel A. M. "Molecular cloning of Atlantic salmon pituitary hormone genes." Thesis, Queen's University Belfast, 1993. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.359115.

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33

Cowin, John. "Molecular cloning and transformation studies in Aspergillus nidulans." Thesis, University of Essex, 1991. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.292601.

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34

Schofield, Miles Alexander. "Molecular cloning of renal cysteine conjugate #beta#-lyase." Thesis, University of Surrey, 1993. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.385104.

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35

Kwok, Ho-yan Amy. "Molecular cloning and characterization of chicken prostaglandin receptors." Click to view the E-thesis via HKUTO, 2008. http://sunzi.lib.hku.hk/hkuto/record/B41508336.

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36

胡可進 and Kejin Hu. "Molecular cloning and characterization of the cathepsin L gene from the marine shrimp metapenaeus ensis." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2003. http://hub.hku.hk/bib/B3124421X.

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37

Churchman, Sarah M. "Cloning and characterization of a novel nitrilase from Rhodococcus ruber NCIMB 40757." Thesis, University of Huddersfield, 2003. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.289409.

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38

Dean, Deyrick Osmond. "Isolation and phenotypic characterisation of deletion mutants of dnaK." Thesis, University of East Anglia, 1991. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.292657.

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39

Zhang, Gaiping. "Bovine IgG Fc receptors." Thesis, University of Hertfordshire, 1994. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.387187.

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40

Millar, N. S. "Molecular cloning and sequence analysis of Newcastle disease virus." Thesis, University of Newcastle Upon Tyne, 1987. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.380750.

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41

Antic, Dragana. "Molecular genetics of hantaviruses: Cloning, sequencing, expression, and morphogenesis." Thesis, University of Ottawa (Canada), 1993. http://hdl.handle.net/10393/7653.

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Molecular characterization of the Seoul 80-39 virus, a prototype of the Seoul serotype, was carried out by cloning and sequencing. The large (L) genomic RNA segment is 6530 nucleotides long. The viral complementary-sense RNA contains a single open reading frame (ORF) from the AUG codon at nucleotide positions 37-39 to the UAA stop codon at nucleotide positions 6490-6492. This ORF could encode a polypeptide of 2151 amino acids (246,662 D) which likely corresponds to the L protein detected in purified viral particles (Elliot et al., 1984) and is assumed to be an RNA-dependent RNA polymerase mole

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42

Fisher, Simon E. "Positional cloning of the gene responsible for Dent's disease." Thesis, University of Oxford, 1995. http://ora.ox.ac.uk/objects/uuid:22f6e7a5-4f00-41c9-a1d3-1b05899f22c0.

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The hypervariable locus DXS255 in human Xp11.22 has a heterozygosity exceeding 90% and has therefore facilitated the localization of several disease genes which map to the proximal short arm of the X chromosome, including the immune deficiency Wiskott-Aldrich syndrome and the eye disorders retinitis pigmentosa, congenital stationary night blindness and Aland Island eye disease. In addition, a microdeletion involving DXS255 has been identified in patients suffering from Dent's disease, a familial X-linked renal tubular disorder which is characterized by low molecular weight proteinuria, hyperca

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43

Pasternak, Stephen H. "Subtractive cloning of cDNAs from motor neuron-like hybrid cells : a strategy for cloning cDNAs from rare cell types." Thesis, McGill University, 1994. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=41742.

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Although motor neurons represent an important and well defined neuronal population, little is known about their unique gene expression. Because of the difficulties of purifying and culturing large numbers of primary motor neurons, we adopted a cloning strategy based on the NSC34 motor neuron-like hybrid cell line. This cell line was developed by fusing dissociated E14 mouse spinal cord cells with N18TG2 neuroblastoma cells and expresses a host of motor neuron markers and traits. We examined a cDNA library of the NSC34 cell line using a subtractive screening strategy in order to isolate cDNA co

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44

Douvile, Elizabeth. "Cloning and characterization of novel kinases from embryonic cells." Thesis, University of Ottawa (Canada), 1994. http://hdl.handle.net/10393/6903.

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Protein tyrosine kinases (PYKs) play key regulatory roles in the control of cell growth and differentiation. Attempts to identify novel PYKs through expression cloning strategies have led to the identification of a novel family of protein kinases, referred to as dual specificity kinases (DSKs). In addition to their immunoreactivity with antiphosphotyrosine antibodies, DSK family members have the ability to phosphorylate serine, threonine as well as tyrosine residues. A novel protein kinase, Esk (Embryonal carcinoma Ser/thr/tyr Kinase), has been isolated from an embryonal carcinoma (EC) cell li

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45

Szatmari, George B. "Cloning and characterization of Mu-like transposable bacteriophage D108." Thesis, McGill University, 1987. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=75453.

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Bacteriophage D108 is a temperate, transposable, mutator phage similar to phage Mu. These heteroimmune phages have 37 kb linear, double-stranded DNA genomes which are over 90% homologous.<br>We have isolated four independent insertions of an entire thermoinducible D108 c ts10 phage genome in the low-copy plasmid pSC101. We also characterized a previously isolated Mu c ts62 insertion in the same plasmid replicon. Fine-structure analysis of these insertions showed that lytically transposing Mu and D108 genomes caused 5 bp duplications of the target site. In addition, we cloned and sequenced the

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46

Lun, Hoi-man. "Characterization and molecular cloning of proteinases of Trichinella spiralis (Nematoda) /." Hong Kong : University of Hong Kong, 2001. http://sunzi.lib.hku.hk/hkuto/record.jsp?B23621989.

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47

Fischer, Roland. "Molecular cloning of a human plasma membrane Ca²⁺-ATPase /." Zürich, 1989. http://e-collection.ethbib.ethz.ch/show?type=diss&nr=8811.

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48

Truesdell, Peter. "Molecular cloning and expression analysis of Pseudaletia unipuncta allatotropin." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1997. http://www.collectionscanada.ca/obj/s4/f2/dsk2/ftp04/mq20707.pdf.

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49

Boyd, Jason. "Molecular cloning and characterization of two Arabidopsis thaliana sulfotransferases." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 2001. http://www.collectionscanada.ca/obj/s4/f2/dsk3/ftp04/MQ64039.pdf.

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50

Nilsson, Isabelle. "The cholecystokinin receptor family : molecular cloning and pharmacological characterization /." Linköping : Univ, 2003. http://www.bibl.liu.se/liupubl/disp/disp2003/med791s.pdf.

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